Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| detection and molecular characterization of tomato yellow leaf curl virus naturally infecting lycopersicon esculentum in egypt. | tomato yellow leaf curl virus (tylcv) infections of tomato crops in egypt were widely spread in 2014. infected symptomatic tomato plants from different governorates were sampled. tylcv strains israel and mild (tylcv-il, tylcv-mild) were identified by multiplex and real-time pcr. in addition, nucleotide sequence analysis of the v1 and v2 protein genes, revealed ten tylcv egyptian isolates (tylcv from ty1 to 10). phylogenetic analysis showed their high degree of relatedness with tylcv-il jordan is ... | 2017 | 28854789 |
| seed transmission of tomato yellow leaf curl virus in white soybean (glycine max). | tomato yellow leaf curl virus (tylcv) infection of the common bean (phaseolus vulgaris) has been reported, but soybean (glycine max) has not previously been identified as a tylcv host. five cultivars of white soybean were agro-inoculated using an infectious tylcv clone. at 30 days post-inoculation, they showed infection rates of 25% to 100%. typical tylcv symptoms were not observed in any inoculated plants. to examine whether tylcv was transmitted in soybean seeds, dna was isolated from bundles ... | 2017 | 28811759 |
| expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen. | dna encoding the coat protein (cp) of an egyptian isolate of tomato yellow leaf curl virus (tylcv) was inserted into the genome of autographa californica nucleopolyhedrovirus (acnpv) under the control of polyhedrin promoter. the generated recombinant baculovirus construct harboring the coat protein gene was characterized using pcr analysis. the recombinant coat protein expressed in infected insect cells was used as a coating antigen in an indirect enzyme-linked immunosorbent assay (elisa) and do ... | 2017 | 28794924 |
| towards area wide management of insect vectored viruses of tomatoes in the bowen district. | the bowen region of northern queensland is an important winter production area for tomatoes in australia. there are three economically important viruses in the region that affect tomato, tomato yellow leaf curl virus (tylcv), tomato spotted wilt virus (tswv) and potato leafroll virus (plrv), which are vectored by whiteflies, thrips and aphids, respectively. an area wide management approach is required to lower the primary inoculum throughout the district. to this end, we undertook investigations ... | 2017 | 28625668 |
| vector development and vitellogenin determine the transovarial transmission of begomoviruses. | the majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. begomoviruses are transmitted by the whitefly bemisia tabaci in a circulative manner and are maintained through a plant-insect-plant cycle. other routes of begomovirus transmission are not clearly known. here, we report that transovarial transmission from female whiteflies to offspring often happens ... | 2017 | 28607073 |
| no evidence of seed transmissibility of tomato yellow leaf curl virus in nicotiana benthamiana. | recent work reported that tomato yellow leaf curl virus (tylcv) is seed-transmissible in tomato, contrary to previous belief. in this work, we explore whether tylcv is also a seed-borne virus in another member of the solanaceae family, the experimental host nicotiana benthamiana. | 2017 | 28471116 |
| the involvement of heat shock proteins in the establishment of tomato yellow leaf curl virus infection. | tomato yellow leaf curl virus (tylcv), a begomovirus, induces protein aggregation in infected tomatoes and in its whitefly vector bemisia tabaci. the interactions between tylcv and hsp70 and hsp90 in plants and vectors are necessity for virus infection to proceed. in infected host cells, hsp70 and hsp90 are redistributed from a soluble to an aggregated state. these aggregates contain, together with viral dna/proteins and virions, hsps and components of the protein quality control system such as ... | 2017 | 28360921 |
| odor, not performance, dictates bemisia tabaci's selection between healthy and virus infected plants. | although, insect herbivores are generally thought to select hosts that favor the fitness of their progeny, this "mother-knows-best" hypothesis may be challenged by the presence of a plant virus. our previous study showed that the whitefly, bemisia tabaci, the obligate vector for transmitting tomato yellow leaf curl virus (tylcv), preferred to settle and oviposit on tylcv-infected rather than healthy host plant, datura stramonium. the performances of b. tabaci larvae and adults were indeed improv ... | 2017 | 28360861 |
| flupyradifurone effectively manages whitefly bemisia tabaci med (hemiptera: aleyrodidae) and tomato yellow leaf curl virus (tylcv) in tomato. | the cotton whitefly bemisia tabaci (gennadius) is among the most important pests of numerous crops and a vector of >100 plant viruses, causing significant crop losses worldwide. managing the pest as well as inhibiting the transmission of major viruses such as tomato yellow leaf curl virus (tylcv) is of utmost importance for sustainable yields. the efficacy on both whiteflies and virus transmission of the novel systemic butenolide insecticide flupyradifurone, was investigated in this study. | 2017 | 28345196 |
| insecticidal effects on the spatial progression of tomato yellow leaf curl virus and movement of its whitefly vector in tomato. | commercial management of whitefly-transmitted tomato yellow leaf curl virus (tylcv) typically relies on insecticide control of whitefly vectors as a first line of defense. we quantified this effect in crop tunnel studies, with validation in a tomato field setting. tomato yellow leaf curl virus-infected and bemisia tabaci (gennadius)-infested source plants were planted at the beginning of tunneled rows to serve as inoculum source, so that movement of whiteflies and tylcv symptoms could be tracked ... | 2017 | 28334339 |
| diversity, distribution, and evolution of tomato viruses in china uncovered by small rna sequencing. | tomato is a major vegetable crop that has tremendous popularity. however, viral disease is still a major factor limiting tomato production. here we report the tomato virome identified through sequencing small rnas of 170 field-grown samples collected in china. a total of 22 viruses were identified including both well-documented and newly detected viruses. the tomato viral community is dominated by a few species, and they exhibit polymorphisms and recombination in the genomes with coldspots and h ... | 2017 | 28331089 |
| impact of cis-acting elements' frequency in transcription activity in dicot and monocot plants. | the production of new cultivars via recombinant dna technology is important in applied agriculture. promoters play fundamental roles in successful transformation and gene expression. fragments of the upstream regulatory region of the movement protein gene of the tomato yellow leaf curl virus (tylcv; two fragments) and watermelon chlorotic stunt virus (wmcsv, two fragments) and one fragment of the coat protein putative promoter of tylcv (cpty-pro) were isolated to assess their abilities to drive ... | 2015 | 28324408 |
| the six tomato yellow leaf curl virus genes expressed individually in tomato induce different levels of plant stress response attenuation. | tomato yellow leaf curl virus (tylcv) is a begomovirus infecting tomato plants worldwide. tylcv needs a healthy host environment to ensure a successful infection cycle for long periods. hence, tylcv restrains its destructive effect and induces neither a hypersensitive response nor cell death in infected tomatoes. on the contrary, tylcv counteracts cell death induced by other factors, such as inactivation of hsp90 functionality. suppression of plant death is associated with the inhibition of the ... | 2017 | 28324352 |
| autophagy functions as an antiviral mechanism against geminiviruses in plants. | autophagy is an evolutionarily conserved process that recycles damaged or unwanted cellular components, and has been linked to plant immunity. however, how autophagy contributes to plant immunity is unknown. here we reported that the plant autophagic machinery targets the virulence factor βc1 of cotton leaf curl multan virus (clcumuv) for degradation through its interaction with the key autophagy protein atg8. a v32a mutation in βc1 abolished its interaction with nbatg8f, and virus carrying βc1( ... | 2017 | 28244873 |
| slmapk3 enhances tolerance to tomato yellow leaf curl virus (tylcv) by regulating salicylic acid and jasmonic acid signaling in tomato (solanum lycopersicum). | several recent studies have reported on the role of mitogen-activated protein kinase (mapk3) in plant immune responses. however, little is known about how mapk3 functions in tomato (solanum lycopersicum l.) infected with tomato yellow leaf curl virus (tylcv). there is also uncertainty about the connection between plant mapk3 and the salicylic acid (sa) and jasmonic acid (ja) defense-signaling pathways. the results of this study indicated that slmapk3 participates in the antiviral response agains ... | 2017 | 28222174 |
| virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species. | surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ... | 2017 | 28220324 |
| clathrin-mediated endocytosis is involved in tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci. the circulative translocation of the virus in the insect is known in its broad line. however, transit of tylcv from the digestive tract into the haemolymph is poorly understood. we studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsrna silencing. we monitored the quantities of ... | 2017 | 28056414 |
| a jasmonate-inducible defense trait transferred from wild into cultivated tomato establishes increased whitefly resistance and reduced viral disease incidence. | whiteflies damage tomatoes mostly via the viruses they transmit. cultivated tomatoes lack many of the resistances of their wild relatives. in order to increase protection to its major pest, the whitefly bemisia tabaci and its transmitted tomato yellow leaf curl virus (tylcv), we introgressed a trichome-based resistance trait from the wild tomato solanum pimpinellifolium into cultivated tomato, solanum lycopersicum. the tomato backcross line bc5s2 contains acylsucrose-producing type-iv trichomes, ... | 2016 | 27920785 |
| elevated o₃ and tylcv infection reduce the suitability of tomato as a host for the whitefly bemisia tabaci. | the effects of elevated atmospheric ozone (o₃) levels on herbivorous insects have been well studied, but little is known about the combined effects of elevated o₃ and virus infection on herbivorous insect performance. using open-top chambers in the field, we determined the effects of elevated o₃ and tomato yellow leaf curl virus (tylcv) infection on wild-type (wt) tomato and 35s tomato (jasmonic acid (ja) defense-enhanced genotype) in association with whitefly, bemisia tabaci gennadius biotype b ... | 2016 | 27916792 |
| the non-canonical tomato yellow leaf curl virus recombinant that displaced its parental viruses in southern morocco exhibits a high selective advantage in experimental conditions. | recombination events are frequently inferred from the increasing number of sequenced viral genomes, but their impact on natural viral populations has rarely been evidenced. tylcv-is76 is a recombinant (begomovirus,geminiviridae) between the israel strain of tomato yellow leaf curl virus (tylcv-il) and the spanish strain of tomato yellow leaf curl sardinia virus (tylcsv-es) that was generated most probably in the late 1990s in southern morocco (souss). its emergence in the 2000s coincided with th ... | 2016 | 27902403 |
| ap2/erf transcription factors involved in response to tomato yellow leaf curly virus in tomato. | tomato yellow leaf curly virus (tylcv), transmitted by the whitefly (), causes leaf curling and yellowing, plant dwarfism, and growth inhibition in tomato ( l.). the apetala2 (ap2) and ethylene response factor (erf) transcription factor (tf) family, the largest plant-specific tf family, was identified to function in plant development and pathogen defense. our study aimed to analyze the mechanism underlying the function of erf (slerf) tfs in response to tylcv infection and improve useful informat ... | 2016 | 27898839 |
| implication of the whitefly bemisia tabaci cyclophilin b protein in the transmission of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) is a single-stranded (ssdna) begomoviruses that causes severe damage to tomato and several other crops worldwide. tylcv is exclusively transmitted by the sweetpotato whitefly, bemisia tabaci in a persistent circulative and propagative manner. previous studies have shown that the transmission, retention, and circulation of tylcv in its vector involves interaction with insect and endosymbiont proteins, which aid in the transmission of the virus, or have a prot ... | 2016 | 27895657 |
| a peptidoglycan recognition protein acts in whitefly (bemisia tabaci) immunity and involves in begomovirus acquisition. | peptidoglycan recognition proteins (pgrps) are multifunctional pattern recognition proteins. here, we report that a pgrp gene, btpgrp, encodes a pgrp from the whitefly bemisia tabaci (meam1) that binds and kills bacteria in vitro. we analyzed btpgrp transcriptional profiling, and the distribution of the cognate protein within the midgut. fungal infection and wasp parasitization induced expression of btpgrp. silencing btpgrp with artificial media amended with dsrna led to reduced expression of a ... | 2016 | 27892529 |
| the contrasting effects of elevated co2 on tylcv infection of tomato genotypes with and without the resistance gene, mi-1.2. | elevated atmospheric co2 typically enhances photosynthesis of c3 plants and alters primary and secondary metabolites in plant tissue. by modifying the defensive signaling pathways in host plants, elevated co2 could potentially affect the interactions between plants, viruses, and insects that vector viruses. r gene-mediated resistance in plants represents an efficient and highly specific defense against pathogens and herbivorous insects. the current study determined the effect of elevated co2 on ... | 2016 | 27881989 |
| comparative analyses of tomato yellow leaf curl virus c4 protein-interacting host proteins in healthy and infected tomato tissues. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. tylcv causes severe problems in sub-tropical and tropical countries, as well as in korea. however, the mechanism of tylcv infection remains unclear, although the function of each viral component has been identified. tylcv c4 codes for a small protein involved in various cellular fun ... | 2016 | 27721687 |
| members of wrky group iii transcription factors are important in tylcv defense signaling pathway in tomato (solanum lycopersicum). | transmitted by the whitefly bemisia tabaci, tomato yellow leaf curly virus (tylcv) has posed serious threats to plant growth and development. plant innate immune systems against various threats involve wrky group iii transcription factors (tfs). this group participates as a major component of biological processes in plants. | 2016 | 27717312 |
| effects of isaria fumosorosea on tylcv (tomato yellow leaf curl virus) accumulation and transmitting capacity of bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is transmitted by the bemisia tabaci pest middle east-asia minor 1 (meam1) in china. isaria fumosorosea is a fungal pathogen of b. tabaci. however, the effects of fungal infection on tylcv expression and transmission by meam1 are unclear. in this study, potted tomatoes containing second instar nymphs of meam1 were treated with i. fumosorosea ifb01 strain and the relationship between fungal infection in meam1 and its tylcv transmission capacity was investigat ... | 2016 | 27716852 |
| ongoing geographical spread of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) seriously impacts tomato production throughout tropical and sub-tropical regions of the world. it has a broad geographical distribution and continues to spread to new regions in the indian and pacific oceans including australia, new caledonia and mauritius. we undertook a temporally-scaled, phylogeographic analysis of all publicly available, full genome sequences of tylcv, together with 70 new genome sequences from australia, iran and mauritius. this reveale ... | 2016 | 27619929 |
| next-generation sequencing and genome editing in plant virology. | next-generation sequencing (ngs) has been applied to plant virology since 2009. ngs provides highly efficient, rapid, low cost dna, or rna high-throughput sequencing of the genomes of plant viruses and viroids and of the specific small rnas generated during the infection process. these small rnas, which cover frequently the whole genome of the infectious agent, are 21-24 nt long and are known as vsrnas for viruses and vd-srnas for viroids. ngs has been used in a number of studies in plant virolo ... | 2016 | 27617007 |
| tomato yellow leaf curl virus: no evidence for replication in the insect vector bemisia tabaci. | begomovirus ssdna plant virus (family geminiviridae) replication within the bemisia tabaci vector is controversial. transovarial transmission, alteration to whitefly biology, or detection of viral transcripts in the vector are proposed as indirect evidence of replication of tomato yellow leaf curl virus (tylcv). recently, contrasting direct evidence has been reported regarding the capacity of tylcv to replicate within individuals of b. tabaci based on quantitave pcr approaches. time-course exper ... | 2016 | 27476582 |
| the whitefly bemisia tabaci knottin-1 gene is implicated in regulating the quantity of tomato yellow leaf curl virus ingested and transmitted by the insect. | the whitefly bemisia tabaci is a major pest to agricultural crops. it transmits begomoviruses, such as tomato yellow leaf curl virus (tylcv), in a circular, persistent fashion. transcriptome analyses revealed that b. tabaci knottin genes were responsive to various stresses. upon ingestion of tomato begomoviruses, two of the four knottin genes were upregulated, knot-1 (with the highest expression) and knot-3. in this study, we examined the involvement of b. tabaci knottin genes in relation to tyl ... | 2016 | 27455309 |
| infectivity, effects on helper viruses and whitefly transmission of the deltasatellites associated with sweepoviruses (genus begomovirus, family geminiviridae). | begomoviruses (family geminiviridae) are whitefly-transmitted viruses with single-stranded dna genomes that are frequently associated with dna satellites. these satellites include non-coding satellites, for which the name deltasatellites has been proposed. although the first deltasatellite was identified in the late 1990s, little is known about the effects they have on infections of their helper begomoviruses. recently a group of deltasatellites were identified associated with sweepoviruses, a g ... | 2016 | 27453359 |
| comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and tylcv infection. | tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. the virus is a widespread plant virus that causes important economic losses in tomatoes. genetic engineering strategies have increasingly been adopted to improve the resistance of tomatoes to tylcv. | 2016 | 27436092 |
| deciphering the biology of deltasatellites from the new world: maintenance by new world begomoviruses and whitefly transmission. | deltasatellites are small noncoding dna satellites associated with begomoviruses. the study presented here has investigated the biology of two deltasatellites found in wild malvaceous plants in the new world (nw). infectious clones of two nw deltasatellites (from malvastrum coromandelianum and sidastrum micranthum) and associated begomoviruses were constructed. infectivity in nicotiana benthamiana and their natural malvaceous hosts was assessed. the nw deltasatellites were not able to spread aut ... | 2016 | 27400152 |
| the salicylic acid-mediated release of plant volatiles affects the host choice of bemisia tabaci. | the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) causes serious crop losses worldwide by transmitting viruses. we have previously shown that salicylic acid (sa)-related plant defenses directly affect whiteflies. in this study, we applied exogenous sa to tomato plants in order to investigate the interaction between sa-induced plant volatiles and nonviruliferous b. tabaci b and q or b- and q-carrying tomato yellow leaf curl virus (tylcv). the results showed that exogenous sa caused ... | 2016 | 27376280 |
| association of tomato leaf curl sudan virus with leaf curl disease of tomato in jeddah, saudi arabia. | tomato is an important vegetable crop and its production is adversely affected by leaf curl disease caused by begomovirus. leaf curl disease is a serious concern for tomato crops caused by begomovirus in jeddah, kingdom of saudi arabia. tomato leaf curl disease has been shown to be mainly caused either by tomato leaf curl sudan virus or tomato yellow leaf curl virus as well as tomato leaf curl oman virus. many tomato plants infected with monopartite begomoviruses were also found to harbor a symp ... | 2016 | 27366765 |
| the autophagy pathway participates in resistance to tomato yellow leaf curl virus infection in whiteflies. | macroautophagy/autophagy plays an important role against pathogen infection in mammals and plants. however, little has been known about the role of autophagy in the interactions of insect vectors with the plant viruses, which they transmit. begomoviruses are a group of single-stranded dna viruses and are exclusively transmitted by the whitefly bemisia tabaci in a circulative manner. in this study, we found that the infection of a begomovirus, tomato yellow leaf curl virus (tylcv) could activate ... | 2016 | 27310765 |
| natural occurrence of tomato leaf curl new delhi virus in iranian cucurbit crops. | the main areas for field-grown vegetable production in iran were surveyed during the years of 2012-2014 to determine the occurrence of begomoviruses infecting these crops. a total of 787 leaf samples were collected from vegetables and some other host plants showing virus-like symptoms and tested by an enzyme-linked immunosorbent assay (elisa) using polyclonal antibodies produced against tomato yellow leaf curl virus (tylcv). according to the elisa results, 81 samples (10.3%) positively reacted w ... | 2016 | 27298595 |
| analysis of the mild strain of tomato yellow leaf curl virus, which overcomes ty-2 gene-mediated resistance in tomato line h24. | in tomato line h24, an isolate of the mild (mld) strain of tomato yellow leaf curl virus (tylcv-mld [jr:kis]) overcomes ty-2 gene-mediated resistance and causes typical symptoms of tomato yellow leaf curl disease (tylcd). no systemic infection with visible symptoms or accumulation of viral dna in the upper leaves was observed in h24 challenged with another isolate, tylcv-il (tylcv-il [jr:osaka]), confirming that h24 is resistant to the il strain. to elucidate the genomic regions that cause the b ... | 2016 | 27231006 |
| tomato yellow leaf curl virus benefits population growth of the q biotype of bemisia tabaci (gennadius) (hemiptera: aleyrodidae). | plant viruses can directly influence their insect vectors, and indirectly through their shared host plant, altering their behavior and performance in a mutualistic or rather antagonistic manner. one of the most studied begomovirus, tomato yellow leaf curl virus (tylcv), may also facilitate the expansion of its vector, the whitefly bemisia tabaci (gennadius). considering the likely expansion of the disease and its major vector, we studied the direct and the indirect effects of a mediterranean iso ... | 2014 | 27193818 |
| corrigendum: tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperatures. | 2016 | 27166264 | |
| cotton leaf curl multan betasatellite dna as a tool to deliver and express the human b-cell lymphoma 2 (bcl-2) gene in plants. | the betasatellite dna associated with cotton leaf curl multan virus (clcumb) contains a single complementary-sense orf, βc1, which is a pathogenicity determinant. clcumb was able to replicate in plants in the presence of diverse helper geminiviruses, including tomato leaf curl virus-australia (tlcv-au), iranian isolate of tomato yellow leaf curl virus (tylcv-[ab]), and beet curly top virus (bctv-svr), and can be used as a plant gene delivery vector. to test the hypothesis that clcumb has the pot ... | 2016 | 27041273 |
| effects of host plant factors on the bacterial communities associated with two whitefly sibling species. | although discrepancy in the specific traits and ecological characteristics of bemisia tabaci between species are partially attributed to the b. tabaci-associated bacteria, the factors that affect the diversity of b. tabaci-associated bacteria are not well-understood. we used the metagenomic approach to characterize the b. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria. | 2016 | 27008327 |
| evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics. | plant viruses in the genus begomovirus, family geminiviridae often cause substantial crop losses. these viruses have been emerging in many locations throughout the tropics and subtropics. like many plant viruses, they are often not recognized by plant diagnostic clinics due in large part to the lack of rapid and cost effective assays. an isothermal amplification assay, recombinase polymerase amplification (rpa), was evaluated for its ability to detect three begomoviruses and for its suitability ... | 2016 | 27000806 |
| the role of corchorus in spreading of tomato yellow leaf curl virus on tomato in jeddah, saudi arabia. | corchorus (corchorus capsularis l. and corchorus olitorius l.) is one of the most important fiber crops grown in tropical and subtropical regions throughout the world. field survey was conducted and naturally infected leaf samples were collected from corchorus and tomato plants in jeddah, saudi arabia. the causal virus was transmitted by whiteflies to tomato plants and begomovirus infection was confirmed by polymerase chain reaction. the complete viral genome and associated betasatellites were a ... | 2016 | 26925440 |
| the c2 protein of tomato yellow leaf curl sardinia virus acts as a pathogenicity determinant and a 16-amino acid domain is responsible for inducing a hypersensitive response in plants. | the role of the c2 protein in the pathogenicity of tomato yellow leaf curl sardinia virus (tylcsv) was investigated. here we report that agrobacterium-mediated transient expression of tylcsv c2 resulted in a strong hypersensitive response (hr) in nicotiana benthamiana, n. tabacum, and arabidopsis thaliana, with induction of plant cell death and production of h2o2. since hr is not evident in plants infected by tylcsv, it is expected that tylcsv encodes a gene (or genes) that counters this respons ... | 2016 | 26826600 |
| tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperatures. | cultured tomatoes are often exposed to a combination of extreme heat and infection with tomato yellow leaf curl virus (tylcv). this stress combination leads to intense disease symptoms and yield losses. the response of tylcv-susceptible and resistant tomatoes to heat stress together with viral infection was compared. the plant heat-stress response was undermined in tylcv infected plants. the decline correlated with the down-regulation of heat shock transcription factors (hsfs) hsfa2 and hsfb1, a ... | 2016 | 26792235 |
| eugenol confers resistance to tomato yellow leaf curl virus (tylcv) by regulating the expression of slper1 in tomato plants. | tomato yellow leaf curl virus (tylcv) is one of the most devastating plant diseases, and poses a significant agricultural concern because of the lack of an efficient control method. eugenol is a plant-derived natural compound that has been widely used as a food additive and in medicine. in the present study, we demonstrated the potential of eugenol to enhance the resistance of tomato plants to tylcv. the anti-tylcv efficiency of eugenol was significantly higher than that of moroxydine hydrochlor ... | 2016 | 26776605 |
| tomato yellow leaf curl virus (tylcv-il): a seed-transmissible geminivirus in tomatoes. | tomato yellow leaf curl virus (tylcv) is one of the most well-known tomato-infecting begomoviruses and transmitted by bemisia tabaci. seed transmission has previously been reported for some rna viruses, but tylcv has not previously been described as a seed-borne virus. in 2013 and 2014, without whitefly-mediated transmission, tylcv was detected in young tomato plants germinated from fallen fruits produced from tylcv-infected tomato plants in the previous cultivation season. in addition, tylcv-is ... | 2016 | 26743765 |
| field trial and molecular characterization of rnai-transgenic tomato plants that exhibit resistance to tomato yellow leaf curl geminivirus. | rna interference (rnai) is a widely used approach to generate virus-resistant transgenic crops. however, issues of agricultural importance like the long-term durability of rnai-mediated resistance under field conditions and the potential side effects provoked in the plant by the stable rnai expression remain poorly investigated. here, we performed field trials and molecular characterization studies of two homozygous transgenic tomato lines, with different selection markers, expressing an intron- ... | 2016 | 26713353 |
| genome-wide analysis of tomato long non-coding rnas and identification as endogenous target mimic for microrna in response to tylcv infection. | recently, a large number of long noncoding rnas (lncrnas) have emerged as important regulators of many biological processes in animals and plants. however, how lncrnas function during plant dna virus infection is largely unknown. we performed strand-specific paired-end rna sequencing of tomato samples infected with tomato yellow leaf curl virus (tylcv) with three biological replicates. overall, we predicted 1565 lncrnas including long intergenic ncrnas (lincrnas) and natural antisense transcript ... | 2015 | 26679690 |
| tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci to tomato and other crops. tylcv proteins are endangered by the host defenses. we have analyzed the capacity of the tomato plant and of the whitefly insect vector to degrade the six proteins encoded by the tylcv genome. tomato and whitefly demonstrated the highest proteolytic activity in the fractions containing soluble proteins, less-in large protein aggregates; a significant decrease of tylcv prot ... | 2016 | 26654789 |
| rapid accumulation and low degradation: key parameters of tomato yellow leaf curl virus persistence in its insect vector bemisia tabaci. | of worldwide economic importance, tomato yellow leaf curl virus (tylcv, begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. the dna begomoviruses (geminiviridae) are transmitted by the whitefly species complex bemisia tabaci. although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of tylcv in b. tabaci raised the question of their possible replication in the vector. we moni ... | 2015 | 26625871 |
| crispr/cas9-mediated viral interference in plants. | the crispr/cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. recently, crispr/cas9 has been used for targeted genome editing in diverse eukaryotic species. | 2015 | 26556628 |
| insecticides promote viral outbreaks by altering herbivore competition. | while the management of biological invasions is often characterized by a series of single-specieg decisions, invasive species exist within larger food webs. these biotic interactions can alter the impact of control/eradication programs and may cause suppression efforts to inadvertently facilitate invasion spread and impact. we document the rapid replacement of the invasive bemisia middle east-asia minor i (meam1) cryptic biotype by the cryptic mediterranean (med) biotype throughout china and dem ... | 2015 | 26552266 |
| temporal effects of a begomovirus infection and host plant resistance on the preference and development of an insect vector, bemisia tabaci, and implications for epidemics. | persistent plant viruses, by altering phenotypic and physiological traits of their hosts, could modulate the host preference and fitness of hemipteran vectors. a majority of such modulations increase vector preference for virus-infected plants and improve vector fitness, ultimately favouring virus spread. nevertheless, it remains unclear how these virus-induced modulations on vectors vary temporally, and whether host resistance to the pathogen influences such effects. this study addressed the tw ... | 2015 | 26529402 |
| monitoring the dynamics of emergence of a non-canonical recombinant of tomato yellow leaf curl virus and displacement of its parental viruses in tomato. | recombinant viruses are increasingly being reported but the dynamics of their emergence is rarely documented. a new recombinant tomato yellow leaf curl virus (tylcv-is76) was detected for the first time in 2010 in southern morocco (souss). an original diagnostic tool was needed to fit its unusual recombination profile. although is76 was detected following the appearance of tylc symptoms on tolerant tomato plants, symptoms could not be associated to is76 or to a synergy with criniviruses. accordi ... | 2015 | 26519598 |
| effectiveness of cyantraniliprole for managing bemisia tabaci (hemiptera: aleyrodidae) and interfering with transmission of tomato yellow leaf curl virus on tomato. | cyantraniliprole is the second xylem-systemic active ingredient in the new anthranilic diamide class. greenhouse (2006), growth chamber (2007), and field studies (2009-2010) were conducted to determine the efficacy of cyantraniliprole for managing bemisia tabaci (gennadius) biotype b and in interfering with transmission of tomato yellow leaf curl virus (tylcv) by this whitefly. cyantraniliprole applied as soil treatments (200 sc) or foliar sprays (100 od) provided excellent adult whitefly contro ... | 2015 | 26470209 |
| manipulation of host quality and defense by a plant virus improves performance of whitefly vectors. | pathogen-mediated interactions between insect vectors and their host plants can affect herbivore fitness and the epidemiology of plant diseases. while the role of plant quality and defense in mediating these tripartite interactions has been recognized, there are many ecologically and economically important cases where the nature of the interaction has yet to be characterized. the bemisia tabaci (gennadius) cryptic species mediterranean (med) is an important vector of tomato yellow leaf curl viru ... | 2015 | 26470098 |
| a novel route controlling begomovirus resistance by the messenger rna surveillance factor pelota. | tomato yellow leaf curl virus (tylcv) is a devastating disease of tomato (solanum lycopersicum) that can be effectively controlled by the deployment of resistant cultivars. the tylcv-resistant line ty172 carries a major recessive locus for tylcv resistance, designated ty-5, on chromosome 4. in this study, the association between 27 polymorphic dna markers, spanning the ty-5 locus, and the resistance characteristics of individual plants inoculated with tylcv in 51 segregating recombinant populati ... | 2015 | 26448569 |
| tomato yellow leaf curl virus differentially influences plant defence responses to a vector and a non-vector herbivore. | plants frequently engage in simultaneous interactions with diverse classes of biotic antagonists. differential induction of plant defence pathways by these antagonists, and interactions between pathways, can have important ecological implications; however, these effects are currently not well understood. we explored how tomato yellow leaf curl virus (tylcv) influenced the performance of its vector (bemisia tabaci) and a non-vector herbivore (tetranychus urticae) occurring separately or together ... | 2016 | 26436779 |
| evaluating weeds as hosts of tomato yellow leaf curl virus. | bemisia tabaci (gennadius) biotype b transmits tomato yellow leaf curl virus (tylcv), which affects tomato production globally. prompt destruction of virus reservoirs is a key component of virus management. identification of weed hosts of tylcv will be useful for reducing such reservoirs. the status of weeds as alternate hosts of tylcv in florida remains unclear. in greenhouse studies, b. tabaci adults from a colony reared on tylcv-infected tomato were established in cages containing one of four ... | 2015 | 26314055 |
| immunity to tomato yellow leaf curl virus in transgenic tomato is associated with accumulation of transgene small rna. | gene silencing is a natural defense response of plants against invading rna and dna viruses. the rna post-transcriptional silencing system has been commonly utilized to generate transgenic crop plants that are "immune" to plant virus infection. here, we applied this approach against the devastating dna virus tomato yellow leaf curl virus (tylcv) in its host tomato (solanum lycopersicum l.). to generate broad resistance to a number of different tylcv viruses, three conserved sequences (the interg ... | 2015 | 26255053 |
| in silico analysis of gene content in tomato genomic regions mapped to the ty-2 resistance gene. | tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. previous studies have shown that ty-2 is an important resistance gene located between molecular markers c2_at2g28250 (82.3 cm) and t0302 (89.0 cm), and exhibits strong resistance to tomato yellow leaf curl virus in asia. in this study, ty-2 candidate genes were subjected to bioinformatic analysis for the sequenced tomato genome. we identified 69 genes between molecular markers c2_at2g28250 and t0302, ... | 2015 | 26214476 |
| replication of tomato yellow leaf curl virus in its whitefly vector, bemisia tabaci. | tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted exclusively by the whitefly bemisia tabaci in a persistent, circulative manner. replication of tylcv in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. following 8 h of acquisition on tylcv-infected tomato plants or purified virions and then transfer to non-tylcv-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first fe ... | 2015 | 26178995 |
| synthesis and biological activity evaluation of novel amino acid derivatives as potential elicitors against tomato yellow leaf curl virus. | disease caused by tomato yellow leaf curl virus (tylcv) brings serious production losses of cultivated tomato worldwide. in our previous study, two novel amino acid derivatives exerted satisfactory antiviral activities against tylcv. in this study, the variation of tylcv, the transcriptional expression level of ty-1 and the enzyme activities of pod and ppo in tomato were monitored after treatment with two amino acid derivatives to illustrate the antiviral mechanism. the results showed the sympto ... | 2015 | 26162434 |
| assessing the genetic variation of ty-1 and ty-3 alleles conferring resistance to tomato yellow leaf curl virus in a broad tomato germplasm. | tomato yellow leaf curl virus (tylcv) hampers tomato production worldwide. our previous studies have focussed on mapping and ultimately cloning of the tylcv resistance genes ty-1 and ty-3. both genes are derived from solanum chilense and were shown to be allelic. they code for an rna-dependent rna polymerase (rdr) belonging to the rdrγ type defined by a dfdgd catalytic domain. in this study, we first fine-mapped the tylcv resistance in s. chilense la1932, la1960 and la1971. results showed that c ... | 2017 | 26028987 |
| transmission of tomato yellow leaf curl virus by bemisia tabaci as affected by whitefly sex and biotype. | bemisia tabaci is a serious pest of vegetables and other crops worldwide. the most damaging and predominant b. tabaci biotypes are b and q, and both are vectors of tomato yellow leaf curl virus (tylcv). previous research has shown that q outperforms b in many respects but comparative research is lacking on the ability of b and q to transmit viruses. in the present study, we tested the hypothesis that b and q differ in their ability to transmit tylcv and that this difference helps explain tylcv o ... | 2015 | 26021483 |
| recessive resistance derived from tomato cv. tyking-limits drastically the spread of tomato yellow leaf curl virus. | the tomato yellow leaf curl disease (tylcd) causes severe damage to tomato (solanum lycopersicum l.) crops throughout tropical and subtropical regions of the world. tylcd is associated with a complex of single-stranded circular dna plant viruses of the genus begomovirus (family geminiviridae) transmitted by the whitefy bemisia tabaci gennadius (hemiptera: aleyrodidae). the tomato inbred line tx 468-rg is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. tyki ... | 2015 | 26008699 |
| tomato plant cell death induced by inhibition of hsp90 is alleviated by tomato yellow leaf curl virus infection. | to ensure a successful long-term infection cycle, begomoviruses must restrain their destructive effect on host cells and prevent drastic plant responses, at least in the early stages of infection. the monopartite begomovirus tomato yellow leaf curl virus (tylcv) does not induce a hypersensitive response and cell death on whitefly-mediated infection of virus-susceptible tomato plants until diseased tomatoes become senescent. the way in which begomoviruses evade plant defences and interfere with c ... | 2016 | 25962748 |
| first complete genomic characterization and phylogeny of a new recombinant of tomato yellow leaf curl virus (genus begomovirus, family geminiviridae) from kuwait. | while whitefly-transmitted begomoviruses are economically important constraints to tomato production in kuwait, little is known about genomic features of these viruses from kuwait. a begomovirus isolated from severely diseased tomatoes, collected over a two-year period in the main tomato-growing areas of kuwait, was characterized at the molecular level. the complete genomic sequence of the begomovirus was determined, and phlylogeographic studies were conducted to better understand genetic divers ... | 2015 | 25951968 |
| the tomato yellow leaf curl virus v2 protein forms aggregates depending on the cytoskeleton integrity and binds viral genomic dna. | the spread of tomato yellow leaf curl virus (tylcv) was accompanied by the formation of coat protein (cp) aggregates of increasing size in the cytoplasm and nucleus of infected tomato (solanum lycopersicum) cells. in order to better understand the tylcv-host interaction, we investigated the properties and the subcellular accumulation pattern of the non-structural viral protein v2. cp and v2 are the only sense-oriented genes on the virus circular single-stranded dna genome. similar to cp, v2 loca ... | 2015 | 25940862 |
| rna interference-based resistance in transgenic tomato plants against tomato yellow leaf curl virus-oman (tylcv-om) and its associated betasatellite. | tomato yellow leaf curl virus (tylcv), a monopartite begomovirus (family geminiviridae) is responsible for heavy yield losses for tomato production around the globe. in oman at least five distinct begomoviruses cause disease in tomato, including tylcv. unusually, tylcv infections in oman are sometimes associated with a betasatellite (tomato leaf curl betasatellite [tolcb]; a symptom modulating satellite). rna interference (rnai) can be used to develop resistance against begomoviruses at either t ... | 2015 | 25890080 |
| the tomato yellow leaf curl virus (tylcv) v2 protein inhibits enzymatic activity of the host papain-like cysteine protease cyp1. | the viral v2 protein is one of the key factors that tomato yellow leaf curl geminivirus (tylcv), a major tomato pathogen worldwide, utilizes to combat the host defense. besides suppressing the plant rna silencing defense by targeting the host sgs3 component of the silencing machinery, v2 also interacts with the host cyp1 protein, a papain-like cysteine protease likely involved in hypersensitive response reactions. the biological effects of the v2-cyp1 interaction, however, remain unknown. we add ... | 2015 | 25797621 |
| assessment of the genetic diversity of tomato yellow leaf curl virus. | the objective of the present study was to analyze the genetic diversity of tomato yellow leaf curl virus (tylcv). representative tylcv sequences were searched in the national center for biotechnology information database. comprehensive analysis of tylcv was performed using bioinformatics by examining gene structure, sequence alignments, phylogeny, gc content, and homology. forty-eight representative tylcv sequences were selected from 48 regions in 29 countries. the results showed that all tylcv ... | 2015 | 25729988 |
| genome-wide analysis of bhlh transcription factor and involvement in the infection by yellow leaf curl virus in tomato (solanum lycopersicum). | the basic helix-loop-helix (bhlh) proteins are a superfamily of transcription factors that can bind to specific dna target sites. they have been well characterized in model plants such as arabidopsis and rice and have been shown to be important regulatory components in many different biological processes. however, no systemic analysis of the bhlh transcription factor family has yet been reported in tomatoes. tomato yellow leaf curl virus (tylcv) threatens tomato production worldwide by causing l ... | 2015 | 25652024 |
| tylcv-is movement in planta does not require v2 protein. | tomato yellow leaf curl virus (tylcv), a major tomato pathogen causing extensive crop losses, is a whitefly-transmitted geminivirus. v2 mutants of tylcv-is and related viruses tend to induce symptomless infection with attenuated viral dna levels, while accumulating close to wild-type dna levels in protoplasts, suggesting v2 as a movement protein. the discovery of plant-silencing mechanisms and viral silencing suppressors, v2 included, led us to reconsider v2׳s involvement in viral movement. we s ... | 2015 | 25644513 |
| management of whitefly-transmitted viruses in open-field production systems. | whiteflies are a key pest of crops in open-field production throughout the tropics and subtropics. this is due in large part to the long and diverse list of devastating plant viruses transmitted by these vectors. open-field production provides many challenges to manage these viruses and in many cases adequate management has not been possible. diseases caused by whitefly-transmitted viruses have become limiting factors in open-field production of a wide range of crops, i.e., bean golden mosaic di ... | 2014 | 25410102 |
| new insecticides for management of tomato yellow leaf curl, a virus vectored by the silverleaf whitefly, bemisia tabaci. | greenhouse studies using a randomized complete block design were carried out to evaluate the effect of six insecticides on transmission of tomato yellow leaf curl virus (tylcv) by the silverleaf whitefly, bemisia tabaci biotype b gennadius (hemiptera: aleyrodidae) to tomato, lycopersicon esculentum (miller) (solanales: solanaceae), seedlings that were inoculated with whiteflies from a tylcv colony in cages 3, 7, or 14 d after treatment with insecticide. the purpose was to reveal differences in r ... | 2014 | 25368089 |
| quantification and localization of watermelon chlorotic stunt virus and tomato yellow leaf curl virus (geminiviridae) in populations of bemisia tabaci (hemiptera, aleyrodidae) with differential virus transmission characteristics. | bemisia tabaci (gennadius) is one of the economically most damaging insects to crops in tropical and subtropical regions. severe damage is caused by feeding and more seriously by transmitting viruses. those of the genus begomovirus (geminiviridae) cause the most significant crop diseases and are transmitted by b. tabaci in a persistent circulative mode, a process which is largely unknown. to analyze the translocation and to identify critical determinants for transmission, two populations of b. t ... | 2014 | 25365330 |
| a rapid and efficient method for construction of an infectious clone of tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus, is responsible for one of the most devastating viral diseases in tomato-growing countries and is becoming a serious problem in many subtropical and tropical countries. the climate in korea is getting warmer and developing subtropical features in response to global warming. these changes are being accompanied by tylcv, which is now becoming a large problem in the korean tomato industry. the most effective way to reduce dama ... | 2014 | 25289018 |
| a sensitive method for the quantification of virion-sense and complementary-sense dna strands of circular single-stranded dna viruses. | circular single-stranded dna (ssdna) viruses are the smallest viruses known to infect eukaryotes. high recombination and mutation rates have conferred these viruses with an evolutionary potential that has facilitated their emergence. their damaging effects on livestock (circoviruses) and crops (geminiviruses and nanoviruses), and the ubiquity of anelloviruses in human populations and other mammalian species, have resulted in increased interest in better understanding their epidemiology and infec ... | 2014 | 25241765 |
| specific cells in the primary salivary glands of the whitefly bemisia tabaci control retention and transmission of begomoviruses. | the majority of plant viruses are vectored by arthropods via persistent-circulative or noncirculative transmission. previous studies have shown that specific binding sites for noncirculative viruses reside within the stylet or foregut of insect vectors, whereas the transmission mechanisms of circulative viruses remain ambiguous. here we report the critical roles of whitefly primary salivary glands (psgs) in the circulative transmission of two begomoviruses. the middle east asia minor 1 (meam1) s ... | 2014 | 25210181 |
| tomato yellow leaf curl virus resistance by ty-1 involves increased cytosine methylation of viral genomes and is compromised by cucumber mosaic virus infection. | tomato yellow leaf curl virus (tylcv) and related begomoviruses are a major threat to tomato production worldwide and, to protect against these viruses, resistance genes from different wild tomato species are introgressed. recently, the ty-1 resistance gene was identified, shown to code for an rna-dependent rna polymerase and to be allelic with ty-3. here we show that upon tylcv challenging of resistant lines carrying ty-1 or ty-3, low virus titers were detected concomitant with the production o ... | 2014 | 25136118 |
| effects of plant virus and its insect vector on encarsia formosa, a biocontrol agent of whiteflies. | in this study, we investigated the tritrophic interactions among a persistently transmitted plant virus, tomato yellow leaf curl virus (tylcv), its insect vector, the sweetpotato whitefly bemisia tabaci, and a parasitoid, encarsia formosa gahan, one of the most extensively used biological control agents. as an emerging invasive pest worldwide, the two most damaging whiteflies are b. tabaci b and q cryptic species. on healthy tomato plants, parasitoid-induced mortality was significantly higher in ... | 2014 | 25096549 |
| fine mapping of the tomato yellow leaf curl virus resistance gene ty-2 on chromosome 11 of tomato. | resistances to begomoviruses, including bipartite tomato mottle virus and monopartite tomato yellow leaf curl virus (tylcv), have been introgressed to cultivated tomato (solanum lycopersicum) from wild tomato accessions. a major gene, ty-2 from s. habrochaites f. glabratum accession "b6013," that confers resistance to tylcv was previously mapped to a 19-cm region on the long arm of chromosome 11. in the present study, approximately 11,000 plants were screened and nearly 157 recombination events ... | 2014 | 25076841 |
| three-way interactions between the tomato plant, tomato yellow leaf curl virus, and bemisia tabaci (hemiptera: aleyrodidae) facilitate virus spread. | plant defense responses can greatly affect plant viruses and their herbivore vectors. the current article reports on plant defense responses involving jasmonic acid (ja), salicylic acid (sa), and proteinase inhibitor (pi) in the three-way interaction between tomato plants, tomato yellow leaf curl virus, and the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae). the results showed that feeding by viruliferous b. tabaci increases the longevity and fecundity of nonviruliferous b. tabaci ... | 2014 | 25026648 |
| water balance, hormone homeostasis, and sugar signaling are all involved in tomato resistance to tomato yellow leaf curl virus. | vacuolar water movement is largely controlled by membrane channels called tonoplast-intrinsic aquaporins (tip-aqps). some tip-aqp genes, such as tip2;2 and tip1;1, are up-regulated upon exposure to biotic stress. moreover, tip1;1 transcript levels are higher in leaves of a tomato (solanum lycopersicum) line resistant to tomato yellow leaf curl virus (tylcv) than in those of a susceptible line with a similar genetic background. virus-induced silencing of tip1;1 in the tomato resistant line and th ... | 2014 | 24989233 |
| differential identification of three species of curtovirus using loop-mediated isothermal amplification. | rapid and sensitive detection methods for three species of curtovirus were developed using a loop-mediated isothermal amplification (lamp) technique. a universal primer set for detecting the three main species of curtovirus at the same time, and three kinds of species-specific primer sets were designed and used for lamp reactions. results from the lamp reactions were visualized both by color changes after adding sybr green i staining dye and by dna laddering on agarose gel electrophoresis. the o ... | 2014 | 24957721 |
| genetic diversity, host range, and distribution of tomato yellow leaf curl virus in iran. | tomato yellow leaf curl virus (tylcv) is considered one of the most important tomato pathogens in tropical and subtropical regions including iran. during the years 2007 to 2009, a total number of 510 symptomatic and asymptomatic vegetable, ornamental and weed samples were collected from fields and greenhouses in ten provinces of iran. symptoms included stunting, yellowing, leaf curl and flower senescence. pcr with specific primers showed tylcv infection in 184 samples (36%) such as cucumber, pep ... | 2014 | 24957717 |
| bemisia tabaci q carrying tomato yellow leaf curl virus strongly suppresses host plant defenses. | the concurrence of tomato yellow leaf curl virus (tylcv) with the spread of its vector bemisia tabaci q rather than b in china suggests a more mutualistic relationship between tylcv and q. here, we investigated the hypothesis that viruliferous b and q have different effects on plant defenses. we found the fecundity of nonviruliferous b, nonviruliferous q, viruliferous q and viruliferous b was 11.080, 12.060, 10.760, and 11.220 respectively on plants previously attacked by the other biotype, howe ... | 2014 | 24912756 |
| molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern arabia. | tomato leaf curl sudan virus (tolcsdv) is a single-stranded dna begomovirus of tomato that causes downward leaf curl, yellowing, and stunting. leaf curl disease results in significant yield reduction in tomato crops in the nile basin. tolcsdv symptoms resemble those caused by tomato yellow leaf curl virus, a distinct and widespread begomovirus originating in the middle east. in this study, tomato samples exhibiting leaf curl symptoms were collected from gezira, sudan. the associated viral genome ... | 2014 | 24890736 |
| upregulation of probing- and feeding-related behavioural frequencies in bemisia tabaci upon acquisition of tomato yellow leaf curl virus. | the behaviour of insect vectors can be altered by the acquisition of plant viruses. bemisia tabaci, which is the vector of tomato yellow leaf curl virus (tylcv), causes damage to susceptible tomato cultivars. here, the frequencies of several behavioural characteristics related to probing and feeding that are exhibited by non-viruliferous (nv) and tylcv-viruliferous (v) adult b. tabaci were compared using a sandwich-type parafilm cage. | 2014 | 24831051 |
| sweet pepper confirmed as a reservoir host for tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation. | tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus, has a single-stranded dna genome. tylcv can induce severe disease symptoms on tomato plants, but other hosts plants such as cucurbits and peppers are asymptomatic. a full-length dna clone of a korean tylcv isolate was constructed by rolling-circle amplification from tylcv-infected tomatoes in korea. to assess relative susceptibility of sweet pepper varieties to tylcv, 19 cultivars were inoculated with cloned tylcv by agro- ... | 2014 | 24777825 |
| only minimal regions of tomato yellow leaf curl virus (tylcv) are required for replication, expression and movement. | the il-60 platform, consisting of a disarmed form of tomato yellow leaf curl virus (tylcv) and auxiliary components, was previously developed as a nontransgenic universal vector system for gene expression and silencing that can express an entire operon in plants. il-60 does not allow rolling-circle replication; hence, production of viral single-stranded (ss) dna progeny is prevented. we used this double-stranded (ds) dna-restricted platform (uncoupled from the dsdna→ssdna replication phase of pr ... | 2014 | 24719195 |
| rapid spread of a recently introduced virus (tomato yellow leaf curl virus) and its vector bemisia tabaci (hemiptera: aleyrodidae) in liaoning province, china. | in liaoning province, china, tomato yellow leaf curl virus (tylcv) was first detected in 2009 and in only four counties. to quantify the spread of tylcv and to identify potential factors influencing its spread in liaoning province, we assayed for tylcv within 1,055 whiteflies (bemisia tabaci (gennadius) complex) from 74 populations and 29 counties in 2011. the b. tabaci species of these individuals was determined based on molecular markers. tylcv was found in 13 counties (donggang, liaoyang, kaz ... | 2014 | 24665690 |
| histochemical visualization of ros and antioxidant response to viral infections of vegetable crops grown in azerbaijan. | extremes of environmental conditions, such as biotic stresses, strongly affect plant growth and development and may adversely affect photosynthetic process. virus infection is especially problematic in crops, because unlike other diseases, its impact cannot be reduced by phytosanitary treatments. the vegetable crops (solanum lycopеrsicum l, cucurbita melo l., cucumis sativus l., piper longum l., solánum melongéna l., vicia faba l.) showing virus-like symptoms were collected from fields located i ... | 2014 | 24661407 |
| location of symbionts in the whitefly bemisia tabaci affects their densities during host development and environmental stress. | bacterial symbionts often enhance the physiological capabilities of their arthropod hosts and enable their hosts to expand into formerly unavailable niches, thus leading to biological diversification. many arthropods, including the worldwide invasive whitefly bemisia tabaci, have individuals simultaneously infected with symbionts of multiple genera that occur in different locations in the host. this study examined the population dynamics of symbionts that are located in different areas within b. ... | 2014 | 24632746 |
| implication of the bacterial endosymbiont rickettsia spp. in interactions of the whitefly bemisia tabaci with tomato yellow leaf curl virus. | numerous animal and plant viruses are transmitted by arthropod vectors in a persistent, circulative manner. tomato yellow leaf curl virus (tylcv) is transmitted by the sweet potato whitefly bemisia tabaci. we report here that infection with rickettsia spp., a facultative endosymbiont of whiteflies, altered tylcv-b. tabaci interactions. a b. tabaci strain infected with rickettsia acquired more tylcv from infected plants, retained the virus longer, and exhibited nearly double the transmission effi ... | 2014 | 24600010 |
| frequency-dependent assistance as a way out of competitive exclusion between two strains of an emerging virus. | biological invasions are the main causes of emerging viral diseases and they favour the co-occurrence of multiple species or strains in the same environment. depending on the nature of the interaction, co-occurrence can lead to competitive exclusion or coexistence. the successive fortuitous introductions of two strains of tomato yellow leaf curl virus (tylcv-mld and tylcv-il) in réunion island provided an ideal opportunity to study the invasion of, and competition between, these worldwide emergi ... | 2014 | 24598426 |