Publications

relationship between tomato yellow leaf curl viruses and the whitefly vector.	tomato yellow leaf curl virus (tylcv) and tomato leaf curl virus (tolcv) are the currently known begomoviruses in uganda. the relationship with their whiteflies (bemisia tabaci) vector and its management were not known in uganda. a direct relationship was expected between these begomoviruses and whiteflies at buwama in mpigi district of uganda. farmer practices were expected to have limited efficacy. to investigate this, a completely randomised block design was used for all trials with six treat ...	2007	18396846
functional analysis of bipartite begomovirus coat protein promoter sequences.	we demonstrate that the al2 gene of cabbage leaf curl virus (calcuv) activates the cp promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for tomato golden mosaic virus (tgmv). binding studies indicate that sequences mediating repression and activation of the tgmv and calcuv cp promoter specifically bind different nuclear factors common to nicotiana benthamiana, spinach and tomato. however, chromatin immunoprecipitation demonstrates that tgmv al2 ...	2008	18430450
chinese tomato yeliow ieaf curl virus- a new species of geminivirus.	chine tomato yellow leaf curl virus (tylcv-chi) and other geminiviruses were analysed with 20 monoclonai antibodies. it was shown that tylcv-chi is serclogicaily close to chinese tabacco ieaf curl virus (tblcv-chi). the fragment of tylcv-chi dna including the common region (cr), n-terminal of coat protein gene and av1 gene was amplified by pcr and cloned, and its dna sequence was determined. these raults showed that tylcv-chi is different from other known geminiviruses in the world, and is a new ...	1998	18726248
generation of plants resistant to tomato yellow leaf curl virus by using artificial zinc-finger proteins.	previously, we designed an artificial zinc-finger protein (azp) for blocking a replication protein (rep) of beet severe curly top virus (bsctv) from binding to its replication origin and demonstrated that transgenic arabidopsis plants expressing the azp are completely resistant to the virus infection. here we applied the azp technology to tomato yellow leaf curl virus (tylcv) infective to an important agricultural crop, tomato. we designed and constructed an azp binding to the direct repeat to b ...	2008	18776317
resistance to monopartite begomoviruses associated with the bean leaf crumple disease in phaseolus vulgaris controlled by a single dominant gene.	abstract tomato yellow leaf curl virus (tylcv) and tomato yellow leaf curl málaga virus are monopartite begomoviruses (genus begomovirus, family geminiviridae) that infect common bean (phaseolus vulgaris), causing bean leaf crumple disease (blcd). this disease was found to be widespread in southern spain and causes stunted growth, flower abortion, and leaf and pod deformation in common bean plants. commercial yield losses of up to 100% occur. in the present study, we have identified and characte ...	2005	18943015
tomato yellow leaf curl virus in the dominican republic: characterization of an infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts.	abstract epidemics of tomato yellow leaf curl disease (tylcd) in the dominican republic in the early to mid-1990s resulted in catastrophic losses to processing tomato production. as part of an integrated management approach to tylcd, the complete nucleotide sequence of a full-length infectious clone of an isolate of tomato yellow leaf curl virus (tylcv) from the dominican republic (tylcv-[do]) was determined. the tylcv-[do] genome was nearly identical in sequence (>97%) and genome organization t ...	2002	18943022
phenotypic expression, stability, and inheritance of a recessive resistance to monopartite begomoviruses associated with tomato yellow leaf curl disease in tomato.	tomato-infecting begomoviruses comprise a complex of monopartite and bipartite virus species that cause severe yield and quality losses worldwide. therefore, the availability of wide spectrum resistance for begomovirus control is desirable. however, limited sources of resistance are available. in this study, three tomato inbred lines with resistance to bipartite begomoviruses of brazil were tested for resistance to monopartite begomoviruses associated with the tomato yellow leaf curl disease (ty ...	2008	18943231
pepper (capsicum annuum) is a dead-end host for tomato yellow leaf curl virus.	abstract tomato yellow leaf curl (tylc) is one of the most devastating pathogens affecting tomato (lycopersicon esculentum) worldwide. the disease is caused by a complex of begomovirus species, two of which, tomato yellow leaf curl sardinia virus (tylcsv) and tomato yellow leaf curl virus (tylcv), are responsible for epidemics in southern spain. tylcv also has been reported to cause severe damage to common bean (phaseolus vulgaris) crops. pepper (capsicum annuum) plants collected from commercial ...	2005	18943307
sequence analysis and classification of apparent recombinant begomoviruses infecting tomato in the nile and mediterranean basins.	abstract numerous whitefly-transmitted viral diseases of tomato have emerged in countries around the nile and mediterranean basins the last 20 years. these diseases are caused by monopartite geminiviruses (family gemini viridae) belonging to the genus begomovirus that probably resulted from numerous recombination events. the molecular biodiversity of these viruses was investigated to better appreciate the role and importance of recombination and to better clarify the phylogenetic relationships a ...	2005	18943321
effect of host plant resistance to tomato yellow leaf curl virus (tylcv) on virus acquisition and transmission by its whitefly vector.	abstract the effect that tomato yellow leaf curl virus (tylcv)-infected resistant tomato plants may have on virus epidemiology was studied. four tomato genotypes that exhibit different levels of viral resistance, ranging from fully susceptible to highly resistant, served as tylcv-infected source plants. viral acquisition and transmission rates by white-flies following feeding on the different source plants were evaluated. tylcv transmission rate by whiteflies that had fed on infected source plan ...	2001	18943336
development of a scale for evaluation of tomato yellow leaf curl virus resistance level in tomato plants.	abstract we have developed a scale of differential hosts that enables the determination and comparison of level of resistance to tomato yellow leaf curl virus (tylcv) expressed by resistant tomato lines or by individual plants in a segregating population. the scale is composed of seven different homozygous tomato genotypes that exhibit different levels of tylcv resistance, ranging from fully susceptible to highly resistant. the differential hosts composing the scale were inoculated with tylcv un ...	2006	18943674
use of tomato yellow leaf curl virus (tylcv) rep gene sequences to engineer tylcv resistance in tomato.	abstract tomato yellow leaf curl virus (tylcv), a member of the genus begomovirus (family geminiviridae), causes severe losses in tomato production in the tropics and subtropics. in order to generate engineered resistance, eight different constructs of the tylcv replication-associated protein (rep) and c4 gene sequences were tested in transformed tomato inbred lines. transgenic plants were screened for resistance to tylcv using viruliferous whiteflies. no symptoms were observed and no tylcv geno ...	2004	18943768
capsicum species: symptomless hosts and reservoirs of tomato yellow leaf curl virus.	abstract five capsicum species were tested for susceptibility to tomato yellow leaf curl virus (tylcv) and the mild strain of tylcv (tylcv-mld). tylcv was able to infect 30 of 55 genotypes of c. annuum, one of six genotypes of c. chinense, one of two genotypes of c. baccatum, and the only genotype of c. frutescens tested but was unable to infect the one genotype of c. pubescens tested. this is the first evidence for the susceptibility of c. baccatum, c. chinense, and c. frutescens to tylcv. unli ...	2006	18944303
rate of tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly bemisia tabaci.	abstract whiteflies (bemisia tabaci, biotype b) were able to transmit tomato yellow leaf curl virus (tylcv) 8 h after they were caged with infected tomato plants. the spread of tylcv during this latent period was followed in organs thought to be involved in the translocation of the virus in b. tabaci. after increasing acquisition access periods (aaps) on infected tomato plants, the stylets, the head, the midgut, a hemolymph sample, and the salivary glands dissected from individual insects were s ...	2001	18944393
watermelon chlorotic stunt virus from the sudan and iran: sequence comparisons and identification of a whitefly-transmission determinant.	abstract the genomes of two watermelon chlorotic stunt virus (wmcsv) isolates, one from the sudan and one from iran, were cloned and sequenced. sequence relationship with other geminiviruses characterizes wmcsv as a typical eastern hemisphere geminivirus with a bipartite genome. the two geographically distant wmcsv isolates from africa and the middle east share a very high overall sequence similarity: 98% between their dna-a and 96% between their dna-b components, and their respective capsid pro ...	2000	18944543
displacement of tomato yellow leaf curl virus (tylcv)-sr by tylcv-is in tomato epidemics in spain.	abstract a progressive displacement of tomato yellow leaf curl virus (tylcv)-sr by tylcv-is was observed in tomato epidemics in southern spain based on incidence data of both virus species obtained during surveys conducted between 1996 and 1998. ecological factors that might be involved in such a displacement, such as competition of tylcv-sr and tylcv-is in tomato, transmission by local biotypes (b and q) of bemisia tabaci, and presence in weeds and alternate crops, have been analyzed. no select ...	1999	18944659
tomato breeding lines resistant and tolerant to tomato yellow leaf curl virus issued from lycopersicon hirsutum.	abstract two tomato yellow leaf curl virus (tylcv)-resistant plants from accessions la1777 and la386 of the wild tomato species lycopersicon hirsutum have been crossed. the resulting resistant f1 plants were crossed with the domesticated tomato l. esculentum, and a series of selfing was performed. at each generation, individuals were selected for resistance (no symptoms and undetectable viral dna) and tolerance (no symptoms but with detectable viral dna) following controlled massive and repeated ...	1998	18944868
quantitation over time of tomato yellow leaf curl geminivirus dna in its whitefly vector.	abstract the amount of tomato yellow leaf curl geminivirus (tylcv) dna that accumulated in the vector bemisia tabaci was studied by quantitative chemiluminescent dot-blot assay, using digoxigenin-labeled specific dna probes. large groups of female whiteflies were allowed to feed for 4, 12, 24, or 48 h on tylcv-infected tomato plants and then were transferred to tylcv-immune cucumber plants. insects were sampled at different times during and after acquisition access and tested for tylcv-dna conte ...	1997	18945078
a novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses.	viruses of the genus begomovirus (geminiviridae) are emerging economically important plant viruses with a circular, single-stranded dna genome. previous studies have shown that geminiviruses and rna viruses exhibit similar mutation frequencies, although geminiviruses are replicated by host dna polymerases and rna viruses by their own virus-encoded error-prone rna-dependent rna-polymerase. however, the phenotypic effects of naturally occurring mutations have never been extensively investigated in ...	2008	18976479
evaluation of two gene-silencing constructs for resistance to tomato yellow leaf curl viruses in nicotiana benthamiana plants.	infiltration of agrobacterium tumefaciens into intact plant leaves of n. benthamiana was used to test the efficiency of two virus-based silencing constructs conferring resistance to the closely related begomoviruses. the constructs contained the most conserved sequences of the coat protein (cp) gene and replication-associated protein (rep) gene of tomato yellow leaf curl sardinia virus (sicily strain) (tylcsv-[sic]). both constructs formed a hairpin structure that enhanced the post-transcription ...	2008	18999888
genetic diversity and distribution of tomato-infecting begomoviruses in iran.	the incidence and severity of tomato leaf curl disease (tlcd) is increasing worldwide. here we assess the diversity and distribution within tomato producing areas of iran of begomoviruses that cause this disease. tomato with typical tlcd symptoms and asymptomatic weeds were collected in 2005 and 2006 and tested for the presence of begomovirus dna using polymerase chain reaction (pcr). analysis of cloned and sequenced pcr products revealed that both mono- and bipartite begomoviruses are associate ...	2009	19112612
dsrna with 5' overhangs contributes to endogenous and antiviral rna silencing pathways in plants.	in plants, sgs3 and rna-dependent rna polymerase 6 (rdr6) are required to convert single- to double-stranded rna (dsrna) in the innate rnai-based antiviral response and to produce both exogenous and endogenous short-interfering rnas. although a role for rdr6-catalysed rna-dependent rna polymerisation in these processes seems clear, the function of sgs3 is unknown. here, we show that sgs3 is a dsrna-binding protein with unexpected substrate selectivity favouring 5'-overhang-containing dsrna. the ...	2009	19165150
expressing a whitefly groel protein in nicotiana benthamiana plants confers tolerance to tomato yellow leaf curl virus and cucumber mosaic virus, but not to grapevine virus a or tobacco mosaic virus.	transgenesis offers many ways to obtain virus-resistant plants. however, in most cases resistance is against a single virus or viral strain. we have taken a novel approach based on the ability of a whitefly endosymbiotic groel to bind viruses belonging to several genera, in vivo and in vitro. we have expressed the groel gene in nicotiana benthamiana plants, postulating that upon virus inoculation, groel will bind to virions, thereby interfering with pathogenesis. the transgenic plants were inocu ...	2009	19184338
first record of tomato yellow leaf curl sardinia virus (tylcsv) on pepper in italy.	during a survey in summer 2007, a disease of pepper (capsicum annuum) under plastic tunnels was observed in policoro (matera), on the ionic coast of basilicata region, with a disease incidence in some cases of more than 50%. affected cultivars were eppo and almund (s et g). the diseased plants exhibited light mosaic or mottling, leaf distortion, interveinal and marginal leaf chlorosis, upward curling of leaf margins of older leaves. the causal pathogen was suspected to be a begomovirus due to th ...	2008	19226766
generation and characterization of functional recombinant antibody fragments against tomato yellow leaf curl virus replication-associated protein.	tomato yellow leaf curl virus (tylcv) is a complex of geminivirus species prevalent in the tropics and sub-tropics, which causes severe diseases in economically important crops such as tomato. conventional strategies for disease management have shown little success and new approaches based on genetic engineering need to be considered. we generated two single-chain variable fragment antibodies (scfv-screp1 and scfv-screp2) that bound strongly to continuous epitopes within the tylcv replication-as ...	2008	19226769
recombinant-antibody-mediated resistance against tomato yellow leaf curl virus in nicotiana benthamiana.	tomato yellow leaf curl virus (tylcv) is a geminivirus species whose members cause severe crop losses in the tropics and subtropics. we report the expression of a single-chain variable fragment (scfv) antibody that protected nicotiana benthamiana plants from a prevalent iranian isolate of the virus (tylcv-ir). two recombinant antibodies (scfv-screp1 and scfv-screp2) interacting with the multifunctional replication initiator protein (rep) were obtained from phage display libraries and expressed i ...	2009	19234665
validation of high rates of nucleotide substitution in geminiviruses: phylogenetic evidence from east african cassava mosaic viruses.	whitefly-transmitted geminiviruses are major pathogens of the important crop cassava in africa. the intensive sampling and sequencing of cassava mosaic disease-causing viruses that occurred in the wake of a severe outbreak in central africa (1997-2002) allowed us to estimate the rate of evolution of this virus. east african cassava mosaic virus and related species are obligately bipartite (dna-a and dna-b segments), and these two genome segments have different evolutionary histories. despite the ...	2009	19264617
a severe symptom phenotype in tomato in mali is caused by a reassortant between a novel recombinant begomovirus (tomato yellow leaf curl mali virus) and a betasatellite.	tomato production in west africa has been severely affected by begomovirus diseases, including yellow leaf curl and a severe symptom phenotype, characterized by extremely stunted and distorted growth and small deformed leaves. here, a novel recombinant begomovirus from mali, tomato yellow leaf curl mali virus (tylcmlv), is described that, alone, causes tomato yellow leaf curl disease or, in combination with a betasatellite, causes the severe symptom phenotype. tylcmlv is an old world monopartite ...	2009	19400843
a simple, rapid and inexpensive method for localization of tomato yellow leaf curl virus and potato leafroll virus in plant and insect vectors.	a simple, rapid, inexpensive method for the localization of virus transcripts in plant and insect vector tissues is reported here. the method based on fluorescent in situ hybridization using short dna oligonucleotides complementary to an rna segment representing a virus transcript in the infected plant or insect vector. the dna probe harbors a fluorescent molecule at its 5' or 3' ends. the protocol: simple fixation, hybridization, minimal washing and confocal microscopy, provides a highly specif ...	2009	19406154
molecular dissection of tomato leaf curl virus resistance in tomato line ty172 derived from solanum peruvianum.	tomato yellow leaf curl virus (tylcv) is devastating to tomato (solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. the breeding line ty172, originating from solanum peruvianum, is highly resistant to tylcv. to map quantitative trait loci (qtls) controlling tylcv resistance in ty172, appropriate segregating populations were analyzed using 69 polymorphic dna markers spanning the entire tomato genome. results show that tylcv resistance in ty172 is c ...	2009	19455299
two new natural begomovirus recombinants associated with the tomato yellow leaf curl disease co-exist with parental viruses in tomato epidemics in italy.	two tomato geminivirus species co-exist in protected crops in sicily, tomato yellow leaf curl sardinia virus (tylcsv, found in 1989) and tomato yellow leaf curl virus (tylcv, found in 2002), and mixed infections have been detected. in a field survey conducted in 2004, the viral intergenic region (ir) was amplified from infected plants, and molecules apparently hybrid between the two species were found, but only in plants where one or both parental species were also present. two of these hybrids, ...	2009	19463717
silencing of a single gene in tomato plants resistant to tomato yellow leaf curl virus renders them susceptible to the virus.	a reverse-genetics approach was applied to identify genes involved in tomato yellow leaf curl virus (tylcv) resistance, taking advantage of two tomato inbred lines from the same breeding program-one susceptible (s), one resistant (r-that used solanum habrochaites as the source of resistance. cdna libraries from inoculated and non-inoculated r and s plants were compared, postulating that genes preferentially expressed in the r line may be part of the network sustaining resistance to tylcv. furthe ...	2009	19533378
use of tomato leaf curl virus (tylcv) truncated rep gene sequence to engineer tylcv resistance in tomato plants.	tomato yellow leaf curl disease causes severe losses in tomato production throughout mediterranean countries including tunisia. in order to generate engineered resistance to this disease, an intron-hairpin rna construct harboring a tomato yellow leaf curl sardinia virus (tylcsv) truncated replication-associated protein (rep) gene was used to transform genotype of tomato plants. prepared transgenic plants were agro-inoculated with tunisian infectious strain of tylcsv and screened for the resistan ...	2009	19537910
interaction of tomato yellow leaf curl virus with diverse betasatellites enhances symptom severity.	the complete nucleotide sequence was determined for a begomovirus isolated from tomato exhibiting leaf curling and yellowing symptoms in tochigi prefecture in japan. the genome organization of this virus was similar to those of other old world monopartite begomoviruses. neither a dna betasatellite nor a dna-b component was detected. it had the highest total nucleotide sequence identity (99%) with tomato yellow leaf curl virus-israel[japan:tosa:2005] (tylcv-il[jr:tos:05]) and tylcv-israel[japan:h ...	2009	19575277
molecular characterization and pathogenicity of tomato yellow leaf curl virus in china.	several tomato production regions in china were surveyed for tomato yellow leaf curl disease (tylcd), and 31 tomato leaf samples showing tylcd-like symptoms were collected. the partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (tylcv) was detected in shanghai, zhejiang, jiangsu shandong and hebei provinces of china. the tylcv isolates found in china share high sequence identity ([98%) and have more than 97% sequence identity with tylcvil[ il:reo] ( ...	2009	19590945
resistance-driven selection of begomoviruses associated with the tomato yellow leaf curl disease.	tomato yellow leaf curl disease (tylcd) causes severe damage to tomato crops worldwide. the deployment of host-plant resistance is the most desirable mean to control this disease. however, some concerns exist because it may place a selection pressure on the virus. field and experimental data are provided which suggest that the use of tylcd resistance in tomato crops might have contributed to the emergence of tomato yellow leaf curl virus in the tylcd-associated virus population, a virus species ...	2009	19735681
laboratory evaluation of products to reduce settling of sweetpotato whitefly adults.	the impact of trademarked and commercial products on settling of adults of the sweetpotato whitefly, bemisia tabaci (gennadius), was studied in the laboratory. a no-choice bioassay using leaf disks of tomato, solanum esculentum l., was developed to evaluate the impact of concentration series of products on settling of b. tabaci adults. the concentration of each product that would reduce settling by 50% (sc50) was estimated for each product using standard probit analyses, and the values were comp ...	2009	19736760
construction of plants resistant to tylcv by using artificial zinc-finger proteins.	previously, we have demonstrated that plant dna virus replication could be inhibited in arabidopsis thaliana by using an artificial zinc-finger protein (azp) and created azp-based transgenic a. thaliana resistant to dna virus infection. here we apply the azp technology to tomato yellow leaf curl virus (tylcv) causing serious damage to an important agricultural crop, tomato. an azp was designed to block binding of the tylcv replication protein (rep) to the replication origin. the designed azp had ...	2009	19749370
broomrape can acquire viruses from its hosts.	broomrapes (phelipanche, formerly orobanche) are parasitic plants that physically connect with the vascular systems of their hosts through haustorial structures. in this study, we found that cucumber mosaic virus (cmv), tomato mosaic virus (tomv), potato virus y (pvy), and tomato yellow leaf curl virus (tylcv) translocate from infected host plants to phelipanche aegyptiaca. in order to examine whether these viruses, and specifically cmv, replicate in the parasite, we tested several replication p ...	2009	19821737
tomato yellow leaf curl virus infection of a resistant tomato line with a silenced sucrose transporter gene leht1 results in inhibition of growth, enhanced virus spread, and necrosis.	to identify genes involved in resistance of tomato to tomato yellow leaf curl virus (tylcv), cdna libraries from lines resistant (r) and susceptible (s) to the virus were compared. the hexose transporter leht1 was found to be expressed preferentially in r tomato plants. the role of leht1 in the establishment of tylcv resistance was studied in r plants where leht1 has been silenced using tobacco rattle virus-induced gene silencing (trv vigs). following tylcv inoculation, leht1-silenced r plants s ...	2010	19946703
inhibition of tomato yellow leaf curl virus (tylcv) using whey proteins.	the antiviral activity of native and esterified whey proteins fractions (alpha-lactalbumin, beta-lactoglobulin, and lactoferrin) was studied to inhibit tomato yellow leaf curl virus (tylcv) on infected tomato plants. whey proteins fractions and their esterified derivatives were sprayed into tylcv-infected plants. samples were collected from infected leaves before treatment, 7 and 15 days after treatment for dna and molecular hybridization analysis. the most evident inhibition of virus replicatio ...	2010	20128897
differentiation of tomato yellow leaf curl virus and tomato yellow leaf curl sardinia virus using real-time taqman pcr.	during the past four decades, tomato yellow leaf curl disease has become one of the major constraints in tomato production worldwide. in the mediterranean basin, several isolates from two major begomovirus species are involved in outbreaks and persistent epidemics. a real-time taqman pcr assay was developed and evaluated for the rapid and multiplex detection and differentiation of two begomoviruses often found in mixed infections in the region, tomato yellow leaf curl virus (tylcv) and tomato ye ...	2010	20153376
an epidemiological survey of tylcd in southern sardinia (italy).	tomato yellow leaf curl sardinia virus (tylcsv) and tomato yellow leaf curl virus (tylcv) are among the most virulent pathogens of greenhouse tomatoes in sardinia (italy). to investigate the relationship between seasonal population trends of the vector bemisia tabaci and the dynamics of tylcd spread to susceptible crops, between may and october 2007 we carried out a survey in a tomato growing area located in the south of the island. on three farms specialized in the production of fresh market to ...	2009	20222569
severe outbreak of tomato yellow leaf curl sardinia virus on pepper in southern italy.	during summer and autumn 2008 a severe outbreak of pepper leaf curl disease (plcd) was observed in pepper crops under plastic tunnels in the ionic coast of basilicata region. its incidence reached, in some cases, values close to 50%. the beginning of infections was recorded along the perimeter of the tunnels, where it reached a percentage of almost 100%. the infection then progressively spread towards the central areas of the greenhouses. large populations of whiteflies, identificated as bemisia ...	2009	20222578
comparative post-harvest behaviour of traditional and virus-resistant muchamiel tomatoes.	nowadays, organoleptic quality is the primary objective for almost all tomato breeding programmes. in this study, post-harvest behaviour of a breeding line with genetic resistance to important viruses (tomato mosaic virus, tomato spotted wilt virus and tomato yellow leaf curl virus) has been compared with the original traditional landrace (muchamiel). the breeding line has been obtained by backcrossing, introgressing three resistance genes but aiming to keep the quality characteristics of the tr ...	2010	20355147
an efficient in vitro-inoculation method for tomato yellow leaf curl virus.	tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. to test the infectivity of tylcv in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed.	2010	20429892
inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies.	a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ...	2010	20447420
oligonucleotide microarray-based detection and identification of 10 major tomato viruses.	a dna microarray chip was developed for screening 10 major economically important tomato viruses from infected plants using "combimatrix" platform 40-mer oligonucleotide probes. a total of 279 oligonucleotide virus probes were specific for simultaneous multiple detection, identification, differentiation and/or genotyping of each of the following tomato rna viruses and/or strains and a virus satellite: cucumber mosaic virus, cucumber mosaic virus satellite rna, tomatoinfectiouschlorosisvirus, tom ...	2010	20470828
differential roles of geminivirus rep and ac4 (c4) in the induction of necrosis in nicotiana benthamiana.	summary the replication-associated protein (rep) of two distinct begomoviruses, the bipartite african cassava mosaic virus (acmv) and the monopartite tomato yellow leaf curl virus-china (tylcv-c), elicits a reaction resembling a hypersensitive response (hr), associated with the induction of local necrosis and a systemic burst of hydrogen peroxide production, when expressed from a potato virus x vector in nicotiana benthamiana. transient expression of the acmv rep after agrobacterium infiltration ...	2002	20569353
tomato yellow leaf curl virus, the intracellular dynamics of a plant dna virus.	summary tomato yellow leaf curl virus is a geminivirus, transmitted by whitefly (bemisia tabaci) and causing the most destructive disease of tomato throughout the mediterranean region, the middle east and the tropical regions of africa and central america. affected plants produce either no fruits or a few small fruits. since it is an ssdna virus which replicates in the host cell nucleus, the molecular mechanisms involved in the viral nuclear import have been the focus of our studies in recent ye ...	2003	20569358
tomato yellow leaf curl viruses: ménage à trois between the virus complex, the plant and the whitefly vector.	tomato yellow leaf curl disease (tylcd) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate regions of the world. here, we focus on the interactions through recombination between the different begomovirus species causing tylcd, provide an overview of the interactions with the cellular genes involved in viral replication, and highlight recent progress on the relationships between these viruses and their vector, the whitefly bemisia tabaci. t ...	2010	20618703
the transmission efficiency of tomato yellow leaf curl virus by the whitefly bemisia tabaci is correlated with the presence of a specific symbiotic bacterium species.	tomato yellow leaf curl virus (tylcv) (geminiviridae: begomovirus) is exclusively vectored by the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae). tylcv transmission depends upon a 63-kda groel protein produced by the vector's endosymbiotic bacteria. b. tabaci is a species complex comprising several genetically distinct biotypes that show different secondary-symbiont fauna. in israel, the b biotype harbors hamiltonella, and the q biotype harbors wolbachia and arsenophonus. both biot ...	2010	20631135
expression of artificial micrornas in tomato confers efficient and stable virus resistance in a cell-autonomous manner.	expression of artificial micrornas (amirnas) in plants can target and degrade the invading viral rna, consequently conferring virus resistance. two amirnas, targeting the coding sequence shared by the 2a and 2b genes and the highly conserved 3' untranslated region (utr) of cucumber mosaic virus (cmv), respectively, were generated and introduced into the susceptible tomato. the transgenic tomato plants expressing amirnas displayed effective resistance to cmv infection and cmv mixed with non-targe ...	2011	20835923
genetic diversity of tomato-infecting tomato yellow leaf curl virus (tylcv) isolates in korea.	epidemic outbreaks of tomato yellow leaf curl virus (tylcv) diseases occurred in greenhouse grown tomato (solanum lycopersicum) plants of busan (tylcv-bus), boseong (tylcv-bos), hwaseong (tylcv-hwas), jeju island (tylcv-jeju), and nonsan (tylcv-nons) in korea during 2008-2009. tomato disease by tylcv has never occurred in korea before. we synthesized the full-length genomes of each tylcv isolate from the tomato plants collected at each area and determined their nucleotides (nt) sequences and ded ...	2010	20963475
phylogenetic analysis and inflow route of tomato yellow leaf curl virus (tylcv) and bemisia tabaci in korea.	tomato yellow leaf curl virus (tylcv) is a member of the genus begomovirus of the family geminiviridae, members of which are characterized by closed circular single-stranded dna genomes of 2.7-2.8 kb in length, and include viruses transmitted by the bemisia tabaci whitefly. no reports of tylcv in korea are available prior to 2008, after which tylcv spread rapidly to most regions of the southern korean peninsula (gyeongsang-do, jeolla-do and jeju-do). fifty full sequences of tylcv were analyzed i ...	2010	20981497
the spread of tomato yellow leaf curl virus from the middle east to the world.	the ongoing global spread of tomato yellow leaf curl virus (tylcv; genus begomovirus, family geminiviridae) represents a serious looming threat to tomato production in all temperate parts of the world. whereas determining where and when tylcv movements have occurred could help curtail its spread and prevent future movements of related viruses, determining the consequences of past tylcv movements could reveal the ecological and economic risks associated with similar viral invasions. towards this ...	2010	21060815
an unusual alphasatellite associated with monopartite begomoviruses attenuates symptoms and reduces betasatellite accumulation.	the oman strain of tomato yellow leaf curl virus (tylcv-om) and its associated betasatellite, an isolate of tomato leaf curl betasatellite (tolcb), were previously reported from oman. here we report the isolation of a second, previously undescribed, begomovirus [tomato leaf curl oman virus (tolcomv)] and an alphasatellite from that same plant sample. this alphasatellite is closely related (90 % shared nucleotide identity) to an unusual dna-2-type ageratum yellow vein singapore alphasatellite (ay ...	2010	21084498
effect of a single amino acid substitution in the nls domain of tomato yellow leaf curl virus-israel (tylcv-il) capsid protein (cp) on its activity and on the virus life cycle.	the capsid protein (cp) of tomato yellow leaf curl virus-israel (tylcv-il), encoded by the v1 gene, is the only known component of the viral capsid. three point mutations introduced into the conserved nls region of the cp were investigated. one mutant, in which the arg at position 19 was converted to leu, had the most significant effect on the cp-cp homotypic interaction as well as on cp's interaction with its nuclear receptor karyopherin a1 and with the protein groel. the latter has been sugges ...	2011	21376764
resistance to tomato yellow leaf curl virus accumulation in the tomato wild relative solanum habrochaites associated with the c4 viral protein.	tomato yellow leaf curl disease (tylcd) is a severe threat to tomato crops worldwide and is caused by tomato yellow leaf curl virus (tylcv) and several other begomoviruses (genus begomovirus, family geminiviridae). host plant resistance is the best tylcd control method but limited sources of resistance are available. in this study, two solanum habrochaites tylcd-resistance sources, eelm-388 and eelm-889, were found after a wide germplasm screening and were further characterized. a consistent res ...	2011	21405986
a common set of developmental mirnas are upregulated in nicotiana benthamiana by diverse begomoviruses.	begomoviruses are single-stranded dna viruses that cause economically important diseases of many crops throughout the world and induce symptoms in plants, including enations, leaf curling and stunting, that resemble developmental abnormalities. micrornas (mirnas) are small endogenous rnas that are involved in a variety of activities, including plant development, signal transduction and protein degradation, as well as response to environmental stress, and pathogen invasion.	2011	21447165
ecology and management of whitefly-transmitted viruses of vegetable crops in florida.	a variety of fresh market vegetables, including watermelon and tomato are economically important crops in florida. whitefly-transmitted squash vein yellowing virus (sqvyv) was first identified in squash and watermelon in florida in 2005 and shown to cause a severe decline of watermelon vines as crops approach harvest. florida is most economically impacted by sqvyv, although the virus has been detected more recently in indiana and south carolina. the origin and evolutionary history of sqvyv, one ...	2011	21549768
phylogenetic lineage of tobacco leaf curl virus in korea and estimation of recombination events implicated in their sequence variation.	new strains of tobacco leaf curl virus (tblcv) were isolated from tomato plants in four different local communities of korea, and hence were designated tblcv-kr. phylogenetic analysis of the sequences of the whole genome and of individual orfs of these viruses indicated that they are closely related to the tobacco leaf curl japan virus (tblcjv) cluster, which includes honeysuckle yellow vein virus (hyvv), honeysuckle yellow vein mosaic virus (hyvmv), and tblcjv isolates. four putative recombinat ...	2011	21549772
comparison of phenotypes produced in response to transient expression of genes encoded by four distinct begomoviruses in nicotiana benthamiana and their correlation with the levels of developmental mirnas.	abstract: background: whitefly-transmitted geminiviruses (begomoviruses) are a major limiting factor for the production of numerous dicotyledonous crops throughout the world. begomoviruses differ in the number of components that make up their genomes and association with satellites, and yet they cause strikingly similar phenotypes, such as leaf curling, chlorosis and stunted plant growth. micrornas (mirnas) are small endogenous rnas that regulate plant growth and development. the study described ...	2011	21592402
whitefly resistance traits derived from the wild tomato solanum pimpinellifollium affect the preference and feeding behavior of bemisia tabaci and reduce the spread of tomato yellow leaf curl virus.	breeding of tomato genotypes that limit whitefly (bemisia tabaci) access and feeding might reduce the spread of tomato yellow leaf curl virus (tylcv), a begomovirus (genus begomovirus, family geminiviridae) that is the causal agent of tomato yellow leaf curl disease. tylcv is restricted to the phloem and is transmitted in a persistent manner by b. tabaci. the tomato breeding line abl 14-8 was developed by introgressing type iv leaf glandular trichomes and secretion of acylsucroses from the wild ...	2011	21615206
a novel synthetic quantification standard including virus and internal report targets: application for the detection and quantification of emerging begomoviruses on tomato.	abstract: background: begomovirus is a genus of phytopathogenic single-stranded dna viruses, transmitted by the whitefly bemisia tabaci. this genus includes emerging and economically significant viruses such as those associated with tomato yellow leaf curl disease, for which diagnostic tools are needed to prevent dispersion and new introductions. five real-time pcrs with an internal tomato reporter gene were developed for accurate detection and quantification of monopartite begomoviruses, includ ...	2011	21819593
chromosomal rearrangements between tomato and solanum chilense hamper mapping and breeding of the tylcv resistance gene ty-1.	tomato yellow leaf curl disease, a devastating disease of solanum lycopersicum (tomato), is caused by a complex of begomoviruses generally referred to as tomato yellow leaf curl virus (tylcv). almost all breeding for tylcv resistance has been based on the introgression of the ty-1 resistance locus derived from solanum chilense la1969. knowledge about the exact location of ty-1 on tomato chromosome 6 will help in understanding the genomic organization of the ty-1 locus. in this study, we analyze ...	2011	21883550
V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor.	The V2 protein of Tomato yellow leaf curl China virus (TYLCCNV) was identified as an RNA silencing suppressor by Agrobacterium-mediated co-infiltration. The V2 protein could inhibit local RNA silencing, systemic RNA silencing of the green fluorescent protein (GFP) gene and the spread of a systemic GFP RNA silencing signal. However, the V2 could not interfere with the cell-to-cell spread of RNA silencing. Subcellular localization assay indicated that the V2 protein was distributed in the cytoplas ...	2011	21893116
complex recombination patterns arising during geminivirus coinfections preserve and demarcate biologically important intra-genome interaction networks.	genetic recombination is an important process during the evolution of many virus species and occurs particularly frequently amongst begomoviruses in the single stranded dna virus family, geminiviridae. as in many other recombining viruses it is apparent that non-random recombination breakpoint distributions observable within begomovirus genomes sampled from nature are the product of variations both in basal recombination rates across genomes and in the over-all viability of different recombinant ...	2011	21949649
top 10 plant viruses in molecular plant pathology.	many scientists, if not all, feel that their particular plant virus should appear in any list of the most important plant viruses. however, to our knowledge, no such list exists. the aim of this review was to survey all plant virologists with an association with molecular plant pathology and ask them to nominate which plant viruses they would place in a 'top 10' based on scientific/economic importance. the survey generated more than 250 votes from the international community, and allowed the gen ...	2011	22017770
Pathogenicity and insect transmission of a begomovirus complex between tomato yellow leaf curl virus and Ageratum yellow vein betasatellite.	Tomato yellow leaf curl virus (TYLCV) and Ageratum yellow vein betasatellite (AYVB) are members of the genus Begomovirus (family Geminiviridae). TYLCV and AYVB have been found in Japan over the last 15 years, and are associated with tomato leaf curl and the tomato yellow leaf curl diseases (TYLCD). AYVB is also associated with some monopartite begomoviruses. We have cloned both TYLCV and AYVB and demonstrated that TYLCV can trans-replicate with AYVB in Nicotiana benthamiana and tomato plants. A ...	2011	22105924
silencing of orfs c2 and c4 of tomato yellow leaf curl virus engenders resistant or tolerant plants.	the il-60 system is a transient universal vector system for expression and silencing in plants [1]. this vector has been derived from tomato yellow leaf curl virus (tylcv). the viral intergenic region (ir) is a non-coding short (314 b) sequence separating the viral sense-oriented genes from the complementary-oriented genes. ir carries the viral origin of replication as well as a promoter at each end. placing a gene segment between two irs at opposite orientations followed by trans-activation of ...	2011	22253651
accurate and sensitive diagnosis of geminiviruses through enrichment, high-throughput sequencing and automated sequence identification.	existing diagnostic techniques used to identify plant-infecting dna viruses and their associated molecules are often limited in their specificity and can be challenged by samples containing multiple viruses. we adapted a simple method of amplifying circular viral dna and, in combination with high-throughput sequencing and bioinformatic analysis, used it as a virus diagnostic method. we validated this diagnostic method with a plant sample infected with a tomato yellow leaf curl geminivirus infect ...	2012	22327393
expression of an entire bacterial operon in plants.	multigene expression is required for metabolic engineering, i.e. coregulated expression of all genes in a metabolic pathway for the production of a desired secondary metabolite. to that end, several transgenic approaches have been attempted with limited success. better success has been achieved by transforming plastids with operons. il-60 is a platform of constructs driven from the geminivirus tomato yellow leaf curl virus. we demonstrate that il-60 enables nontransgenic expression of an entire ...	2012	22353575
genome-wide identification of dicer-like, argonaute and rna-dependent rna polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in solanum lycopersicum.	dicer, argonaute and rna-dependent rna polymerase form the core components to trigger rna silencing. although tomato (solanum lycopersicum) is a dicotyledon model plant, no systematic analysis and expression profiling of these genes in tomato has been undertaken previously. in this study, seven dicer-like (sldcls), 15 argonaute (slagos) and six rna-dependent rna polymerase (slrdrs) genes were identified in tomato. these genes were categorized into four subgroups based on phylogenetic analyses. c ...	2012	22406496
[molecular detection of tomato yellow leaf curl virus (tylcv)].	tomato yellow leaf curl virus (tylcv) is currently considered as one of the most devastating viruses in cultivated tomatoes (solanum lycopersicum) worldwide. we reported here the development of a pcr-based method to quickly detect tylcv using the primer pairs (tylcv-f: 5'-acg cat gcc tct aat cca gtg ta-3' and tylcv-r: 5'-cca ata agg cgt aag cgt gta gac-3'), which was designed based on the genome sequence of tylcv. a tylcv-specific band of 543 bp was amplified from infected tomato plants. this pr ...	2012	22425956
acylsucrose-producing tomato plants forces bemisia tabaci to shift its preferred settling and feeding site.	the whitefly bemisia tabaci (genn.) causes dramatic damage to plants by transmitting yield-limiting virus diseases. previous studies proved that the tomato breeding line abl 14-8 was resistant to b. tabaci, the vector of tomato yellow leaf curl disease (tylcd). this resistance is based on the presence of type iv glandular trichomes and acylsucrose production. these trichomes deter settling and probing of b. tabaci in abl 14-8, which reduces primary and secondary spread of tylcd.	2012	22427950
cotton leaf curl multan betasatellite as a plant gene delivery vector trans-activated by taxonomically diverse geminiviruses.	cotton leaf curl multan betasatellite (clcumb) replicates in tobacco, tomato and datura plants in the presence of the helper viruses tomato leaf curl virus-australia, iranian isolates of tomato yellow leaf curl virus, tomato leaf curl karnataka virus, and beet severe curly top virus (bsctv). infectious recombinant clcumb constructs were made in which segments of either the camv 35s or the petunia chsa promoter replaced the clcumb βc1 orf, and these were designated pbinβδc1-35s and pbinβδc1-chsa, ...	2012	22476203
lower incidence and severity of tomato virus in elevated co(2) is accompanied by modulated plant induced defence in tomato.	elevation in atmospheric co(2) concentration broadly affects plant phenology and physiology, and these effects may alter the performance of plant viruses. the effects of elevated co(2) on the susceptibility of tomato plants to tomato yellow leaf curl virus (tylcv) were examined for two successive years in open top chambers (otc) in the field. we experimentally tested the hypothesis that elevated co(2) would reduce the incidence and severity of tylcv on tomato by altering plant defence strategies ...	2012	22512888
rapid spread of tomato yellow leaf curl virus in china is aided differentially by two invasive whiteflies.	tomato yellow leaf curl virus (tylcv) was introduced into china in 2006, approximately 10 years after the introduction of an invasive whitefly, bemisia tabaci (genn.) b biotype. even so the distribution and prevalence of tylcv remained limited, and the economic damage was minimal. following the introduction of q biotype into china in 2003, the prevalence and spread of tylcv started to accelerate. this has lead to the hypothesis that the two biotypes might not be equally competent vectors of tylc ...	2012	22514670
inhibition of binding of tomato yellow leaf curl virus rep to its replication origin by artificial zinc-finger protein.	previously we demonstrated that inhibition of replication-associated protein (rep) binding to its replication origin by artificial zinc-finger proteins (azps) is a powerful method to prevent plant virus infection in vivo. in the present study, we applied the azp technology to tomato yellow leaf curl virus (tylcv), which is a limiting factor in tomato cultivation worldwide. first, we determined 5'-atcggtgt atcggtgt-3' in the 195-bp intergenic region of the tylcv-israel strain, a strain reported f ...	2013	22576255
major tomato viruses in the mediterranean basin.	tomato (solanum lycopersicum l.) originated in south america and was brought to europe by the spaniards in the sixteenth century following their colonization of mexico. from europe, tomato was introduced to north america in the eighteenth century. tomato plants show a wide climatic tolerance and are grown in both tropical and temperate regions around the world. the climatic conditions in the mediterranean basin favor tomato cultivation, where it is traditionally produced as an open-field plant. ...	2012	22682165
a new strain of tomato severe leaf curl virus and a unique variant of tomato yellow leaf curl virus from mexico.	the complete genome sequence of a distinct variant of tomato yellow leaf curl virus-israel (tylcv-il) and the dna-a sequence of a new strain of tomato severe leaf curl virus (toslcv) isolated in san luis potosi, mexico, are described and analyzed. the tylcv-il[mx:slp:11] variant differs from all tylcv-il isolates described so far by a unique 42-nt duplicated sequence comprising a part of the conserved stem-loop element of the virion-strand replication origin and adjacent regulatory sequences. ty ...	2012	22684489
functional analysis of gene-silencing suppressors from tomato yellow leaf curl disease viruses.	tomato yellow leaf curl disease (tylcd) is caused by a complex of phylogenetically related begomovirus spp. that produce similar symptoms when they infect tomato plants but have different host ranges. in this work, we have evaluated the gene-silencing-suppression activity of c2, c4, and v2 viral proteins isolated from the four main tylcd-causing strains in spain in nicotiana benthamiana. we observed varying degrees of local silencing suppression for each viral protein tested, with v2 proteins fr ...	2012	22712505
the tomato yellow leaf curl virus (tylcv) v2 protein interacts with the host papain-like cysteine protease cyp1.	the v2 protein of tomato yellow leaf curl geminivirus (tylcv) is an rna-silencing suppressor that counteracts the innate immune response of the host plant. however, this anti-host defense function of v2 may include targeting of other defensive mechanisms of the plant. specifically, we show that v2 recognizes and directly binds the tomato cyp1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. this binding occurred both ...	2012	22827939
upregulation of temperature susceptibility in bemisia tabaci upon acquisition of tomato yellow leaf curl virus (tylcv).	acquisition of plant viruses has various effects on physiological mechanisms in vector insects. bemisia tabaci is the only known vector of tomato yellow leaf curl virus (tylcv), which is a serious virus affecting tomato cultivars. in this study, the lifespan of q1 biotype was compared between non-viruliferous (nv) and tylcv-viruliferous (v) whiteflies. total lifespan from egg to adult death of nv whiteflies was 62.54 days but 10.64 days shorter in v whiteflies. we investigated the temperature su ...	2012	22841829
a developmentally regulated lipocalin-like gene is overexpressed in tomato yellow leaf curl virus-resistant tomato plants upon virus inoculation, and its silencing abolishes resistance.	to discover genes involved in tomato resistance to tomato yellow leaf curl virus (tylcv), we previously compared cdna libraries from susceptible (s) and resistant (r) tomato lines. among the genes preferentially expressed in r plants and upregulated by tylcv infection was a gene encoding a lipocalin-like protein. this gene was termed solanum lycopersicum virus resistant/susceptible lipocalin (slvrslip). the slvrslip structural gene sequence of r and s plants was identical. slvrslip was expressed ...	2012	22843056
arsenophonus groel interacts with clcuv and is localized in midgut and salivary gland of whitefly b. tabaci.	cotton leaf curl virus (clcuv) (gemininiviridae: begomovirus) is the causative agent of leaf curl disease in cotton plants (gossypium hirsutum). clcuv is exclusively transmitted by the whitefly species b. tabaci (gennadius) (hemiptera: alerodidae). b. tabaci contains several biotypes which harbor dissimilar bacterial endo-symbiotic community. it is reported that these bacterial endosymbionts produce a 63 kda chaperon groel protein which binds to geminivirus particles and protects them from rapid ...	2012	22900008
whitefly-mediated transmission of cotton leaf curl multan betasatellite: evidence for betasatellite encapsidation in coat protein of helper begomoviruses.	cotton leaf curl multan betasatellite (clcumb) is responsible for symptom expression of a devastating disease of cotton in the indian subcontinent. clcumb depends on helper virus replication-associated protein for its replication and on viral coat protein (cp) for its encapsidation. however, no direct evidence of encapsidation of clcumb in viral cp has been available. in the present study, non-viruliferous whiteflies were placed on tomato plants that had been agroinoculated with infectious clone ...	2013	22923008
whitefly population dynamics and evaluation of whitefly-transmitted tomato yellow leaf curl virus (tylcv)-resistant tomato genotypes as whitefly and tylcv reservoirs.	sweetpotato whitefly, bemisia tabaci (gennadius), and whitefly-transmitted tomato yellow leaf curl virus (tylcv) are major threats to tomato production in the southeastern united states. tylcv was introduced to florida from the caribbean islands and has spread to other southern states of the united states. in georgia, in recent years, the incidence of tylcv has been steadily increasing. studies were conducted to monitor population dynamics of whiteflies in the vegetable production belt of georgi ...	2012	22928328
recombination profiles between tomato yellow leaf curl virus and tomato yellow leaf curl sardinia virus in laboratory and field conditions: evolutionary and taxonomic implications.	tomato yellow leaf curl sardinia virus and tomato yellow leaf curl virus have co-existed in italian tomato crops since 2002 and have reached equilibrium, with plants hosting molecules of both species plus their recombinants being the most frequent case. recombination events are studied in field samples, as well as in experimental co-infections, when recombinants were detected as early as 45 days following inoculation. in both conditions, recombination breakpoints were essentially absent in regio ...	2012	22971824
implication of bemisia tabaci heat shock protein 70 in begomovirus-whitefly interactions.	the whitefly bemisia tabaci (gennadius) is a major cosmopolitan pest capable of feeding on hundreds of plant species and transmits several major plant viruses. the most important and widespread viruses vectored by b. tabaci are in the genus begomovirus, an unusual group of plant viruses owing to their small, single-stranded dna genome and geminate particle morphology. b. tabaci transmits begomoviruses in a persistent circulative nonpropagative manner. evidence suggests that the whitefly vector e ...	2012	23015709
molecular identification of a new begomovirus associated with mosaic disease of jatropha curcas l. in nigeria.	we report the complete nucleotide sequence of dna-a of a begomovirus naturally infecting jatropha curcas l. in nigeria. symptoms observed on infected plants were severe mosaic, mottling and blistering of leaves. the virus, which we provisionally name "jatropha mosaic nigeria virus" (jmnv), has a monopartite genome of 2,779 to 2,789 nucleotides. pairwise comparisons of dna-a sequences showed that jmnv had maximum nucleotide sequence identity (72%) with a strain of tomato yellow leaf curl virus. s ...	2013	23074041
progressive aggregation of tomato yellow leaf curl virus coat protein in systemically infected tomato plants, susceptible and resistant to the virus.	tomato yellow leaf curl virus (tylcv) coat protein (cp) accumulated in tomato leaves during infection. the cp was immuno-detected in the phloem associated cells. at the early stages of infection, punctate signals were detected in the cytoplasm, while in the later stages aggregates of increasing size were localized in cytoplasm and nuclei. sedimentation of protein extracts through sucrose gradients confirmed that progress of infection was accompanied by the formation of cp aggregates of increasin ...	2013	23099086
biological invasions of geminiviruses: case study of tylcv and bemisia tabaci in reunion island.	in the last 20 years, molecular ecology approaches have proven to be extremely useful to identify and assess factors associated with viral emerging diseases, particularly in economically and socially important tropical crops such as maize (maize streak disease) and cassava (cassava mosaic disease). molecular ecology approaches were applied in reunion island to analyze the epidemic of tomato yellow leaf curl disease, which has been affecting the island since the end of the 1990s. before the invas ...	2012	23235470
adaptive evolution by recombination is not associated with increased mutation rates in maize streak virus.	single-stranded (ss) dna viruses in the family geminiviridae are proving to be very useful in real-time evolution studies. the high mutation rate of geminiviruses and other ssdna viruses is somewhat mysterious in that their dna genomes are replicated in host nuclei by high fidelity host polymerases. although strand specific mutation biases observed in virus species from the geminivirus genus mastrevirus indicate that the high mutation rates in viruses in this genus may be due to mutational proce ...	2012	23268599
advanced engineering of lipid metabolism in nicotiana benthamiana using a draft genome and the v2 viral silencing-suppressor protein.	the transient leaf assay in nicotiana benthamiana is widely used in plant sciences, with one application being the rapid assembly of complex multigene pathways that produce new fatty acid profiles. this rapid and facile assay would be further improved if it were possible to simultaneously overexpress transgenes while accurately silencing endogenes. here, we report a draft genome resource for n. benthamiana spanning over 75% of the 3.1 gb haploid genome. this resource revealed a two-member nbfad2 ...	2012	23300750
high efficient of females of b-type bemisia tabaci as males in transmitting the whitefly-borne tomato yellow leaf curl virus to tomato plant with q-pcr method confirmation.	it has been previously reported that tylcv can be transmitted from viruliferous males to non-viruliferous females and from viruliferous females to non-viruliferous males, but not between insects of the same sex; female whiteflies transmit tylcv-is with higher efficiency than males through symptoms recognition and viral dna identification in tomato test plants (one insect per plant, with 48 h aap and 48 h iap). however, it remains unclear whether non-infected female and male could obtain same vir ...	2012	23336021
multiple forms of vector manipulation by a plant-infecting virus: bemisia tabaci and tomato yellow leaf curl virus.	for many insect-vectored plant viruses, the relationship between feeding behavior and vector competence may prove integral to an understanding of the epidemiology of the resulting plant disease. while plant-infecting viruses are well known to change host plant physiology in a way that makes them more attractive to vectors, viral manipulation of the vectors themselves has only recently been reported. previous research suggested that the rapid spread of tomato yellow leaf curl virus (tylcv) throug ...	2013	23408638
involvement of the hexose transporter gene leht1 and of sugars in resistance of tomato to tomato yellow leaf curl virus.		2013	23430051
insect symbiont facilitates vector acquisition, retention, and transmission of plant virus.	tomato yellow leaf curl virus (tylcv) was first detected in china in 2006, following the introduction of bemisia tabaci q into china in 2003. since then, the incidence of tylcv in tomato fields in china has greatly increased as has the abundance and distribution of q whiteflies containing the bacterial symbiont hamiltonella with high frequency. this suggested that the symbiont hamiltonella might associate with the transmission efficiency of tylcv by the whitefly vector. here we report the first ...	2013	23455639