| rheumatologic manifestations of human parvovirus b19 infection in adults. initial two-year clinical experience. | during 1987 and 1988, we identified 9 adults at the medical and rheumatology services of the university of iowa hospitals and clinics who had a clinical diagnosis of fifth disease; 8 of the 9 had symptoms of joint involvement. another 12 adults with serologic positivity for anti-parvovirus b19 igm antibody presented with polyarthralgia/polyarthritis. patients were usually found to be seronegative for rheumatoid factor, and none developed nodules or erosive disease. many patients with chronic dis ... | 1990 | 2169746 |
| an immunofluorescence assay for the detection of parvovirus b19 igg and igm antibodies based on recombinant viral antigen. | an indirect immunofluorescence assay for serum igg and igm antibodies to human parvovirus b19 was established using recombinant b19 viral antigen, the capsid protein vp1, which had been produced in a baculovirus expression system. this protein gives a strong and characteristic signal in the immunofluorescence assay, making it a suitable candidate for this test system. the test results showed a good correlation with results obtained with a solid-phase capture radioimmunoassay (cohen et al., 1983) ... | 1990 | 2170436 |
| the human parvovirus b19. | | 1990 | 2171481 |
| diagnostic of parvovirus b19 infection. | an elisa for detecting parvovirus b19 specific igm and igg was established using b19 viral particles as antigen derived from plasma of donors in the incubation phase of erythema infectiosum. at onset of erythema infectiosum anti-b19 igm was detected in titers of 10(-4) and was positive up to 19 weeks. anti-b19 igg could be detected in clinical specimens one week after onset of erythema infectiosum and persisted in titers up to 10(-6) throughout the convalescent period. among 3,289 sera of patien ... | 1990 | 2171483 |
| detection of human parvovirus b19 dna by dot-hybridization and the polymerase chain reaction: applications for diagnosis of infections. | the development of the polymerase chain reaction for the detection of human parvovirus b19 dna in clinical specimens is described. the sensitivity of this detection is compared with detection of virus using radiolabeled dna and rna probes. the polymerase chain reaction was the most sensitive, detecting approximately 10 fg b19 dna. several applications of the polymerase chain reaction for b19 dna detection are discussed: i) detection of b19 dna sequences in paraffin-embedded fetal tissues in a ca ... | 1990 | 2171484 |
| polymerase chain reaction for detection of parvovirus b19 in immunodeficient patients with anemia. | development of a polymerase chain reaction for detection of parvovirus b19 is described. among 14 primer pairs spanning the whole genome, 6 were shown to amplify viral dna at least 10(5) fold and 4 did not amplify non b19-dna to levels detectable by hybridization with a virus specific dna probe. failure to amplify only one b19 isolate with one particular primer combination suggests limited heterogeneity of the sequence among different viruses. examples for the application of the method are prese ... | 1990 | 2171485 |
| the pathogenesis of diseases associated with b19 virus. | b19 virus infection is common in the population and is frequently asymptomatic. however, a viraemia and prompt antibody response occurs in normal individuals and this is associated with mild, non-specific respiratory tract symptoms at the time of the viraemia and/or a rash-illness a week or ten days later. infection of red cell precursors is a regular occurrence and this leads to aplastic crisis if b19 virus infection occurs in an individual with chronic heamolytic anaemia. fetal infection somet ... | 1990 | 2171486 |
| molecular biology and pathogenicity of human and animal parvoviruses. | the so-called autonomous parvoviruses comprise a wide spectrum of viral agents with quite similar physicochemical and biological properties. characteristically, they are small, naked, and extraordinarily stable particles made up of only 2-4 structural proteins and a genome of linear, single-stranded dna consisting of 4500-5600 nts. for replication they depend on cellular helper functions which become available at distinct stages during cellular differentiation (developmentally-dependent function ... | 1990 | 2171487 |
| risk of infection following exposures to human parvovirus b19. | in response to concern about the effect of human parvovirus b19 infection of the fetus, we have developed estimates of the risk of adults becoming infected following b19 exposures at home, in schools or day-care centers, and in hospitals. these estimates can then be used with other data to estimate the risk to the fetus of a b19 exposure during pregnancy. the risk to the fetus equals the rate of maternal susceptibility to infection times the rate of maternal infection following the specific type ... | 1990 | 2171488 |
| human parvovirus b19 and arthritis. | parvovirus b19 has different clinical manifestations dependent on age and sex. in children it is usually a mild febrile exanthematous illness but in adults 60% of women and 30% of men have an arthropathy which may be severe and persistent. the pathogenesis of this arthropathy is not known. our studies establishing the clinical manifestations of parvovirus infection and examining their pathogenesis are reviewed. | 1990 | 2171489 |
| parvovirus b19 infection in pregnancy. | parvovirus b19 infection in pregnancy can cause hydrops fetalis resulting in fetal loss. acute b19 infection was serologically confirmed in 80 pregnant women by elisa. of 80 pregnancies, 4 were terminated. of the remaining 76 pregnancies, no fetal complications were observed in 36 (47.4%), hydrops fetalis occurred in 18 (23.7%) and no further information was available in 22 (28.9%). 15 of 18 (83.3%) fetuses with hydrops died. intrauterine transfusion was performed in the remaining three fetuses ... | 1990 | 2171490 |
| parvovirus b19 infections in pregnancy. | since 1986, 2,279 pregnant women were screened for anti parvovirus b19 igg and igm antibodies. of them 41% were seronegative, 54% had specific igg but no igm antibodies, indicating an infection in earlier life. acute infection could be demonstrated by igm antibody detection in 114 pregnant women (32% in the first, 54% in the second and 14% in the third trimester). hydrops fetalis occurred in 10 of the 114 pregnancies (8.7%). in five of these cases intrauterine exchange transfusion was done and u ... | 1990 | 2171491 |
| successful intrauterine treatment of fetal hydrops caused by parvovirus b19 infection. | the infection with parvovirus b19 during pregnancy causes in 1/3 of the cases an aplastic crisis in the fetus and consecutively generalized fetal hydrops as a result of severe anemia. some cases of fetal hydrops and intrauterine death are reported. in our two cases, the fetal therapy by intrauterine intravasal transfusion was successful and the children developed normal. the techniques and indications for fetal blood sampling and the method of intrauterine intravasal transfusion are explained. | 1990 | 2171492 |
| immunoglobulins in the prophylaxis of parvovirus b19 infection. | | 1990 | 2172397 |
| parvovirus and epidemic arthritis. | five cases of acute polyarthritis in individuals with serologic evidence of recent human parvovirus b19 infection were seen in one medical practice. several individuals had had direct exposure to children with rash, and fifth disease was endemic in the community. all cases were associated with the presence of both igg and igm antibody to human parvovirus. | 1990 | 2172688 |
| [a family with hereditary spherocytosis discovered after an infection with human parvovirus b19]. | hereditary spherocytosis was diagnosed in five of six children in one family after a human parvovirus b19 (b19-virus) infection. the diagnosis was made on the basis of severe anaemia, demonstrable haemolysis, decreased osmotic fragility and an increased number of spherocytes. since in the serum of the patients an increased level of igg and igm antibodies against b19 virus was detectable two weeks after the crisis it was concluded that the b19-virus infection caused the severe anaemia. | 1990 | 2172845 |
| human parvovirus b19. | | 1990 | 2172896 |
| human parvovirus b19 infections in pregnancy. | | 1990 | 2172907 |
| pure red cell aplasia due to parvovirus b19 infection in a man with hiv infection. | infection by parvovirus b19 is common and may become chronic if the patient is immunocompromised, leading to persistent erythroid hypoplasia. parvovirus should be added to the list of pathogens that can complicate the course of hiv infection and should be considered in the evaluation of severe anemia or red cell aplasia in any immunocompromised patient. | 1990 | 2174578 |
| human parvovirus b19 arthropathy associated with desquamation. | | 1990 | 2174648 |
| the prevalence of antibody to human parvovirus b19 in rio de janeiro, brazil. | during 1985 and 1986 serum samples were collected from the rio de janeiro population and examined for the presence of igg antibody to human parvovirus b19. no difference in prevalence was found between males and females. antibody prevalence rose from 35% in children less than five years old to almost 80% in children aged eleven to fifteen years. the antibody prevalence in individuals over 50 years old was over 90%. | 1990 | 2175446 |
| red cell aplasia associated with human parvovirus b19 and hiv infection: failure to respond clinically to intravenous. | | 1990 | 2175617 |
| expression of an antigenic polypeptide of the human parvovirus b19. | the dna fragment of the human parvovirus b19, with 715 nucleotides between nucleotide positions 3141-3856 was expressed in escherichia coli as a beta-galactosidase fusion protein. the plasmid vector pss20d used for this purpose permits cleavage of the viral gene product from the beta-galactosidase moiety by collagenase. after purification by p-aminophenyl-beta-d-thiogalactoside-sepharose and superose, a soluble protein with a molecular mass of 28 kda was isolated. it represents a common part of ... | 1990 | 2175835 |
| [prevalence of anti-human parvovirus antibody in pregnant women]. | human parvovirus b19 (b19) has been shown to be associated with erythema infectiosum. recently, it was reported that when a pregnant woman is infected with b19, the fetus in her uterus sometimes becomes hydropic and results in a stillbirth. but no epidemiologic study of pregnant women in japan has been performed yet. we have established an in vitro propagation system of b19 virions and an assay system for detecting anti-b19 antibody by indirect immunofluorescence (if) staining. we examined the p ... | 1990 | 2179428 |
| antigenic parvovirus b19 coat proteins vp1 and vp2 produced in large quantities in a baculovirus expression system. | two baculovirus expression vectors derived from autographica californica nuclear polyhedrosis virus (acnpv) were prepared containing the complete 2.5 kb coding region for parvovirus b19 coat protein vp1 (acb19vp1l) and the 1.8 kb coding region for vp2 (acb19vp2l), placed under the control of the polyhedrin promoter. the recombinant viruses were used to infect spodoptera frugiperda cells and the proteins expressed were analysed using appropriate antibodies. acb19vp1l-infected cells produced b19 v ... | 1990 | 2188463 |
| parvovirus b19. a workshop organized by the german association against virus diseases. april 1989. | | 1990 | 2222383 |
| [a family with hereditary spherocytosis, discovered following an infection with human parvovirus b19]. | | 1990 | 2255366 |
| nonstructural protein of parvoviruses b19 and minute virus of mice controls transcription. | the genome of the human parvovirus b19 contains a transcriptional promoter (bp06) at map position 6, upstream from the nonstructural protein genes. by cotransfecting hela cells with this promoter cloned before the chloramphenicol acetyltransferase (cat) gene together with a plasmid containing almost the whole b19 genome, we showed that bp06 is transactivated by a b19 gene product. the transactivating viral protein was identified as the nonstructural protein ns-1. ns-1 synthesized in a wheat germ ... | 1990 | 2293668 |
| susceptibility of human erythropoietic cells to b19 parvovirus in vitro increases with differentiation. | b19 human parvovirus is the etiologic agent of transient aplastic crisis. to better understand b19 virus-induced hematopoietic suppression, we studied the host cell range of the virus using in vitro bone marrow cultures. first, b19 virus replication was examined in the presence of various purified cytokines using dna dot blot analysis. replication was detected only in erythropoietin-containing cultures. the other cytokines (granulocyte/macrophage colony-stimulating factor [gm-csf], g-csf, m-csf, ... | 1990 | 2404522 |
| cloning of the human parvovirus b19 genome and structural analysis of its palindromic termini. | we describe the molecular cloning of the entire 5.6-kb single-stranded dna genome of the human parvovirus b19 in bacterial plasmids. stable amplification of the recombinant plasmid dna was achieved in escherichia coli jc8111 but not in hb101 cells. sequence analysis of the cloned dna shows that the terminal 383 nucleotides at each end of the genome are identical inverted repeats. the distal 365 nucleotides of the repeat represent an imperfect palindrome which presumably folds over to form a hair ... | 1990 | 2408228 |
| maternal serum alpha-fetoprotein--a marker of fetal aplastic crisis during intrauterine human parvovirus infection. | in 2 cases of hydrops fetalis and intrauterine death associated with human parvovirus b19 infection that produced very few symptoms during the second trimester of pregnancy, maternal serum alpha-fetoprotein levels were raised, before the ultrasonic detection of hydropic features. fetal blood sampling in 1 case revealed the features of aplastic crisis. a retrospective study of 3 other affected and 11 unaffected cases of b19 infection during pregnancy showed a correlation between raised maternal s ... | 1987 | 2433556 |
| antenatal diagnosis and palliative treatment of non-immune hydrops fetalis secondary to fetal parvovirus b19 infection. | hydrops fetalis was diagnosed at 22 weeks. an ultrasound examination demonstrated cardiomegaly and a fetal blood specimen obtained by cordocentesis revealed thrombocytopenia, anaemia, and neutropenia. fetal paracentesis yielded straw-coloured fluid with electrolytes indicative of a transudate. non-enveloped icosahedral viral particles approximately 23 mm in diameter were visualized in the ascitic fluid by electron microscopy. immune electron microscopy confirmed human parvovirus b19. direct feta ... | 1989 | 2466284 |
| an amplified elisa for the detection of parvovirus b19 igm using monoclonal antibody to fitc. | a new m-antibody capture elisa for the detection of specific igm against parvovirus b19 is described. the test uses a monoclonal anti-fluorescein isothiocyanate (fitc) antibody conjugated to horseradish peroxidase to amplify the positive reactions. serum samples (n = 823) submitted for b19 igm assay were tested in parallel in the new elisa and the standard b19 m-antibody capture radioimmunoassay (macria) test. by both tests b19 igm was detected in 38 (4.6%) samples, and not detected in 771 (94%) ... | 1989 | 2515204 |
| rapid detection of human parvovirus b19 dna by dot-hybridization and the polymerase chain reaction. | the results of a comparison of three dna-detection methods for human parvovirus b19 dna are described. the sensitivity of detection of virus from hybridization assays using 32p-radiolabeled dna and rna probes was compared with a method for enzymatically amplifying specific target dna sequences (polymerase chain reaction). b19 virus dna was detected using a radiolabeled dna probe at serum dilutions of 10(-3), equivalent to approximately 3 pg of viral dna. using radiolabeled rna probes even 0.3 pg ... | 1989 | 2536380 |
| risks associated with human parvovirus b19 infection. | | 1989 | 2536885 |
| leads from the mmwr. risks associated with human parvovirus b19 infection. | | 1989 | 2537435 |
| leads from the mmwr. risks associated with human parvovirus b19 infection. | | 1989 | 2537438 |
| [parvovirus b19 infection: not confined to exanthema of erythema infectiosum]. | the well-known condition of erythema infectiosum is caused by parvovirus b19, a single-stranded dna virus discovered in 1975. the characteristic erythema obviously does not always appear: antibodies are present in 50%-60% of all adults. the virus is transmitted by airborne droplets, and the incubation period is 13-17 days. clinically, there are unspecific prodromes, and aside from exanthema these can take the form of lymphadenopathy, influenza-like symptoms, and arthralgia. parvovirus b19 has a ... | 1989 | 2537803 |
| human parvovirus b19 infection associated with prolonged erythroblastopenia in a leukemic child. | | 1989 | 2537946 |
| prolonged parvovirus b19 infection with severe anaemia in a bone marrow transplant patient. | | 1989 | 2538139 |
| human parvovirus b19 infections in women of childbearing age and within families. | human parvovirus b19 (b19) crosses the placenta causing fetal death. we used an indirect capture enzyme immunoassay to measure igg to b19 in sera of 845 subjects from 2 groups. the first group included 405 women (mean age, 30 years) composed of 85 pediatric nurses and 130 other female hospital employees, 122 women employed caring for preschool children and 68 mothers of preschool children enrolled in day care. twenty-eight percent of all these women were seropositive. seropositivity was unrelate ... | 1989 | 2539583 |
| dot blot hybridization assay of b19 virus dna in clinical specimens. | a nonradioactive dot blot hybridization assay for human parvovirus b19 dna was set up by using a biotin-labeled dna probe and streptavidin-alkaline phosphatase conjugate. the assay was used to examine 4,895 specimens referred for b19 virus diagnosis during 1987. of 48 specimens that gave positive reactions for b19 dna, 41 were confirmed virus positive by electron microscopy (n = 36), radioimmunoassay (n = 26), or counterimmunoelectrophoresis (n = 20). in 7 samples which were not confirmed and in ... | 1989 | 2541166 |
| erythema multiforme in a patient with acute human parvovirus b19 infection. | | 1989 | 2541181 |
| clinical manifestations of human parvovirus b19 in adults. | human parvovirus b19 has been associated with various clinical effects in a number of uncontrolled reports. to define the usual manifestations of b19 infection in adults and the factors that influence them we present a clinicoepidemiological study of an outbreak of b19 infection centered on a junior school. four hundred fifty-three of 475 adults in this community were interviewed and blood was obtained for serological diagnosis. fifty-four cases of recent infection were identified and were hla t ... | 1989 | 2541666 |
| [acute benign arthritis of the hip. a systematic study of serology of parvovirus b19]. | | 1989 | 2542925 |
| embryonic malformations in a case of intrauterine parvovirus b19 infection. | an incomplete embryo of 9 weeks development from a woman infected by human parvovirus b19 during early pregnancy was histologically examined. b19-dna was detected in both embryonic and placental tissue by dot-blot hybridization. focal vascular endothelial damage was found throughout the entire embryo and placenta together with mononuclear infiltrations around the vessels. in the placenta these mononuclear cells belonged for the greater part to the cytotoxic and/or suppressor t-cell group. one ey ... | 1989 | 2543097 |
| [parvovirus infections in dermatology]. | human parvovirus b19 is the causative agent of erythema infectiosum and other atypical (rubelliform) exanthemas. on account of the relatively high prevalence of b19, these infections are rather frequent. they usually occur during childhood, but also up to the age of 30, and there are various clinical manifestations. during pregnancy, b19 infection may cause hydrops of the fetus with ensuing fetal death. anti-b19 igm and igg can be detected by means of elisa or ria; b19-dna is identified by hybri ... | 1989 | 2544061 |
| human parvovirus b19 infection associated with vascular purpura and vasculitis. | | 1989 | 2544532 |
| [vascular purpura and other cardiovascular alterations in children with acute parvovirus b19 infection. report of 3 personal cases]. | most frequent skin manifestation of parvovirus b19 infection is the erythema infectiosum (fifth disease; sticker's disease). vascular purpura (some cases of henoch-schönlein purpura) has been reported in virus infections such as infectious mononucleosis (epstein-barr virus), hepatitis b, cytomegalovirus etc., and human parvovirus b19 could be another cause. clinical course, procedure of diagnostics and therapy in 3 own serologically established cases of vascular purpura or severe alterations of ... | 1989 | 2544763 |
| aplastic crisis caused by b19 virus in a child during induction therapy for acute lymphoblastic leukemia. | a child undergoing induction therapy for acute lymphoblastic leukemia suffered an aplastic crisis associated with b19 virus infection. good response to the antiblastic therapy led to a burst in erythropoiesis favoring high viral replication, which was responsible for strong erythroblastic inhibition and severe viremia. the patient's b19 antibody response became evident very late, probably because of the antiblastic effect of the therapy; nevertheless, recovery was complete in little more than a ... | 1989 | 2545576 |
| infection control measures for human parvovirus b19 in the hospital setting. | | 1989 | 2545772 |
| parvovirus b19 infection associated with reticulocytopenia and chronic autoimmune hemolytic anemia. | a previously healthy 11-year-old white female presented with a coomb's positive hemolytic anemia and reticulocytopenia. the patient was treated with prednisone (6.4 mg/kg/day) and had a stable hemoglobin with subsequent recovery of reticulocytes by 7 days. bone marrow aspiration showed hypercellularity with arrest of red cell maturation. the patient's serum contained specific igm and igg antibodies to parvovirus b19. our report confirms parvovirus b19 as a cause of reticulocytopenia at presentat ... | 1989 | 2546464 |
| human parvovirus b19 infection among hospital staff members after contact with infected patients. | in the spring and summer of 1988, two separate outbreaks of an illness with a rash resembling erythema infectiosum occurred among members of the nursing staff of the children's hospital of philadelphia. the sources were two adolescent patients with sickle cell disease and aplastic crisis who had unsuspected parvovirus infection. tests for igm and igg antibodies to parvovirus b19 were positive in both patients, and electron microscopical examination showed parvovirus-like particles in the early s ... | 1989 | 2548096 |
| pure red-cell aplasia of 10 years' duration due to persistent parvovirus b19 infection and its cure with immunoglobulin therapy. | | 1989 | 2548098 |
| human parvovirus b19. | | 1989 | 2548099 |
| what threat is human parvovirus b19 to the fetus? a review. | | 1989 | 2548566 |
| fetal pathology in human parvovirus b19 infection. | in a current netherlands study on the effects on mother and child of infection with the human parvovirus b19 during pregnancy, 10 pregnancies have been reported. three of them ended before term: two in fetal death and one by elective abortion. in two of these fetuses b19 infection in cells other than those of the erythroid series was demonstrated, and in the one terminated, ocular malformation and extensive inflammatory reactions in all fetal and placental tissues were found. the presence of b19 ... | 1989 | 2548567 |
| aplastic crisis due to human b19 parvovirus infection in red cell pyrimidine-5'-nucleotidase deficiency. | two siblings with chronic hemolytic anemia due to red cell pyrimidine-5'-nucleotidase (p-5'-n) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. the cause of the aplastic crisis was an acute infection with human b19 parvovirus (b19 hpv) as proven by immunoelectron microscopy and dna hybridization. this is the first report on the association of b19-hpv-related aplastic crisis with p-5'-n deficiency. | 1989 | 2549757 |
| seroprevalence of human parvovirus b19 infection in sao tomé and principe, malawi and mascarene islands. | the prevalence of antibodies to human parvovirus b19 (anti-b19 igg) in sera (n = 577) from sao tomé and principe, malawi and mascarene islands (mauritius and rodriguez islands) was determined by antibody capture elisa. the b19 prevalence was 51.5% on sao tomé and principe, 58.4% in malawi, and 55.0% on the mauritius mainland and 2.2% on rodriguez island, indicating that b19 virus is highly prevalent not only in europe, japan and the americas but also in the african region. rodriguez islands has ... | 1989 | 2550018 |
| hepatic disease associated with intrauterine parvovirus b19 infection in a newborn premature infant. | a hydropic premature infant with intrauterine growth retardation died at 4 days of age and was found at necropsy to have advanced liver disease. clinical and serologic findings in mother and infant were consistent with recent parvovirus b19 infection. parvovirus can cause fetal liver disease in animals, and some instances of congenital hepatic dysfunction in man may be due to intrauterine parvovirus b19 infection. | 1989 | 2550601 |
| anti-dna and antilymphocyte antibodies during acute infection with human parvovirus b19. | sera from patients in the acute and recovery stages of parvovirus b19 infection, and from individuals with no detectable antiparvovirus antibody were examined for the presence of anti-dna and antilymphocyte antibodies. sixty-eight percent of individuals recently recovered from parvovirus infection had elevated levels of antidouble stranded (ds) and antisingle stranded (ss) dna antibodies. in addition, a cytotoxic igm antilymphocyte antibody was detected in more than 88% of these same sera. seria ... | 1989 | 2550630 |
| erythema infectiosum in a primary school: investigation of an outbreak in bury. | between january and april 1987, 97 of 302 (32%) children attending a primary school in bury, lancashire, were affected by an exanthematous illness. the duration and extent of the episode caused considerable local interest and anxiety. examination of 6 serum specimens obtained during the subsequent investigation demonstrated evidence of recent human parvovirus b19 infection in 3 children and 1 teacher. no evidence of recent rubella virus infection was found. the investigation of the episode is di ... | 1989 | 2552489 |
| transient erythroblastopenia of childhood due to human parvovirus b19 infection. | | 1989 | 2553096 |
| concurrent outbreaks of influenza and parvovirus b19 in a boys' boarding school. | in the spring term of 1985 there was a protracted outbreak of upper respiratory tract febrile illness consistent with a clinical diagnosis of influenza in a boys' boarding school, which lasted from 23 january to 29 march. although influenza virus infection was confirmed in 89% of cases in the first half of the term, 53% of the cases which occurred in the second half of the term had no evidence of infection with influenza virus. between 5 february and 31 march 28 boys presented with skin rashes c ... | 1989 | 2553464 |
| construction of a recombinant human parvovirus b19: adeno-associated virus 2 (aav) dna inverted terminal repeats are functional in an aav-b19 hybrid virus. | to facilitate genetic analysis of the human pathogenic parvovirus b19, we constructed a hybrid b19 viral genome in which the defective b19 inverted terminal repeats were replaced with the full-length inverted terminal repeats from a nonpathogenic human parvovirus, the adeno-associated virus 2 (aav). the hybrid aav-b19 genome was rescued from a recombinant plasmid and then the dna was replicated upon transfection into adenovirus 2-infected human kb cells in the presence of aav genes coding for pr ... | 1989 | 2554300 |
| [erythroblastopenia in children]. | the occurrence of isolated erythroblastopenia is a common problem in paediatrics, and in most cases three diagnoses may be considered: blackfan-diamond anaemia (congenital erythroblastopenia), transient erythroblastopenia of childhood and erythroblastopenia consecutive to parvovirus b19 infection. these three diseases have distinctive features: age of onset, association with other laboratory and clinical abnormalities and above all, transient or chronic character are of assistance in making an e ... | 1989 | 2554485 |
| [erythroblastopenia caused by parvovirus in siblings, revealing a hereditary spherocytosis. apropos of a case]. | following the occurrence in two siblings of parvovirus b19-related acute transient erythroblastopenia with specific igms, hereditary spherocytosis (hs) was diagnosed for the first time in the two patients as well as in three other members of the family. | 1989 | 2554771 |
| [erythema nodosum associated with parvovirus b19 infection]. | | 1989 | 2555812 |
| polymerase chain reaction assay of parvovirus b19 dna in clinical specimens. | the polymerase chain reaction (pcr) was used to detect parvovirus b19 dna in a panel of sera from individuals recently infected with b19, as demonstrated by the presence of anti-b19 immunoglobulin m. of 95 serum samples, 60 (63%) were found positive by pcr, whereas only 1 was also found positive by dot hybridization. in a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by pcr, and this was also found positive by dot hybridization. ... | 1989 | 2556428 |
| enzyme-linked immunosorbent assay for igg and igm antibodies against human parvovirus b19: use of monoclonal antibodies and viral antigen propagated in vitro. | enzyme-linked immunosorbent assay (elisa) systems for serum igg and igm antibodies to human parvovirus b19 were established by utilizing anti-b19 monoclonal antibodies (mabs) and human plasma b19 antigen. the specificities of igg and igm elisa were confirmed by indirect immunofluorescence staining and western blot immunoassay with panel sera. the series of serum specimens obtained from two b19-infected patients were examined with elisa. the igm antibody titers increased quickly after the onset o ... | 1989 | 2559100 |
| [aplastic crisis due to human parvovirus b19 infection in glucose-6-phosphate dehydrogenase deficiency]. | human parvovirus b19 is known to cause aplastic crisis in patients with hemolytic anemias due to cytotoxic effect of the infection to erythroid progenitor cells. we report here the first case of aplastic crisis by b19 in a patient with glucose-6-phosphate dehydrogenase deficiency. a five-year-old boy was admitted to the hospital because of severe anemia, fever and jaundice. four weeks after admission, he developed erythema infectiosum. b19 infection was confirmed using countercurrent immunoelect ... | 1989 | 2559569 |
| parvovirus b19 infection in patients receiving cancer chemotherapy: the expanding spectrum of disease. | initial reports have suggested that human parvovirus b19 infection in immune-compromised individuals may cause prolonged or chronic bone marrow suppression. we report four children receiving cancer chemotherapy who developed atypical patterns of cutaneous and hematologic disease associated with parvoviral infection. b19 infection was demonstrated by specific serologies. this report suggests that patients undergoing myelosuppressive or immunosuppressive therapy may have unusual manifestations of ... | 1989 | 2559623 |
| persistent parvovirus b19 infections in humans. | | 1989 | 2560110 |
| symptomatic parvovirus b19 infection and heat-treated factor ix concentrate. | | 1989 | 2566038 |
| expression of the human parvovirus b19 protein fused to protein a in escherichia coli: recognition by igm and igg antibodies in human sera. | a 1.4 kb fragment (nucleotides 2430 to 3901) encoding portions of the human parvovirus b19 structural proteins was inserted into the prit2 plasmid expression vector containing the gene encoding staphylococcal protein a under the control of the phage lambda promoter pr. the fusion protein was used to raise antibodies in rabbits. the sera were shown by immune electron microscopy to agglutinate b19 particles and were also shown to recognize the vp2 b19 capsid protein, by western blot analysis. the ... | 1989 | 2584954 |
| a new peptide for human parvovirus b19 antibody detection. | a serological assay for human parvovirus b19 was developed. linear overlapping synthetic peptides were synthesised according to parts of open reading frames 1 and 2. a region at the n-terminus of viral protein vp2 detected serological reactivity in indirect enzyme-linked immunosorbent assays for igg and igm with known seropositive human sera. a cyclized peptide taken from this region, amino acids 284-307, gave the best selective reactivity with seropositive and seronegative sera. the peptide ass ... | 1989 | 2617203 |
| detection of the human parvovirus b19 in a blood donor plasma in rio de janeiro. | | 1989 | 2635755 |
| propagation of human parvovirus b19 in primary culture of erythroid lineage cells derived from fetal liver. | erythroid lineage cells derived from fetal liver were demonstrated to be target cells for human parvovirus b19 infection. b19 virus antigen-positive serum was inoculated into primary cultures containing erythroid lineage cells enriched from fetal liver. the b19 virus antigen was detected on about 5% of cells in the culture by immunofluorescence staining, and the stained cells were identified as erythroid lineage cells by double staining with anti-b19 virus-positive serum and anti-erythroid linea ... | 1989 | 2657097 |
| characterization of monoclonal antibodies against human parvovirus b19. | eleven hybridoma cell lines producing mouse monoclonal antibodies (mabs) against human parvovirus b19 were established. their specificity was as follows. approximately 5% of fetal erythroid cells inoculated with b19 reacted with all the mabs and with anti-b19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. all the mabs recognized both vp-1 (84 kda) and vp-2 (58 kda) capsid proteins of b19 virions propagated in vitro and in vivo by western blottin ... | 1989 | 2671609 |
| detection of parvovirus b19 dna in fetal tissues by in situ hybridisation and polymerase chain reaction. | attempts were made to detect human parvovirus b19-dna by in situ hybridisation and the polymerase chain reaction in placental and fetal tissues from a case of intrauterine fetal death. in the in situ hybridisation experiments radioactive and non-radioactive (labelled with 2-acetyl-aminofluorene, aaf) dna probes were used. b19-dna was detectable in paraffin wax embedded fetal tissue from the liver, heart, lung, brain and thymus. the resolution with the aaf-labelled probes was higher than with the ... | 1989 | 2732347 |
| transient expression of b19 parvovirus gene products in cos-7 cells transfected with b19-sv40 hybrid vectors. | hybrid b19 parvovirus-sv40 origin vectors were transfected into cos-7 cells and replication of these plasmids studied. plasmids that have a frameshift mutation within the nonstructural gene region replicated to high level (copy number approximately 10,000/transfected cell) although somewhat lower than psvod, the sv40 origin vector without b19 sequence (copy number approximately 100,000/transfected cell). however, hybrid b19 parvovirus-sv40 origin vectors that do not contain these frameshift muta ... | 1989 | 2800343 |
| [incidence of parvovirus b19 infection. seroepidemiologic studies]. | anti-b19-igg antibodies were tested for by the elisa method in 768 sera, 76 from children and juveniles, aged 1-15 years, attending the outpatients department of the children's clinic, university of munich, and 692 from persons, aged 18-68 years, attending the blood donor service of the bavarian red cross in munich. 38.4% of sera were positive, with a significant difference between men and women (32.5% vs. 47.5%, p less than or equal to 0.01). a fresh b19 infection was present in 42 subjects by ... | 1987 | 2820687 |
| [association of non-immunologically-induced hydrops fetalis with parvovirus b19 infection]. | five weeks after the onset of clinical symptoms of erythema infectiosum, a 24 year old pregnant woman, gravida i, para 0, suffered a hydrops fetalis which resulted in fetal loss. the maternal infection with the human parvovirus b19 could be confirmed serologically with elisa and immunoblot by detecting specific igm antibodies against b19. in an amniotic sample of the fetus parvovirus b19 dna could be detected by hybridisation. fetal loss was obviously due to a parvovirus b19 infection of the mot ... | 1987 | 2820831 |
| human parvovirus b19 arthropathy in two adults after contact with childhood erythema infectiosum. | an arthropathy has been recently described in association with human parvovirus infection (hpv-b19). human parvovirus b19 has also been implicated as the etiologic agent in erythema infectiosum, a childhood exanthem that may occur in adults in association with joint manifestations. in this study, two adults are described, in whom an acute arthropathy and rash developed after contact with children with erythema infectiosum. | 1987 | 2821807 |
| human parvovirus b19 infection and juvenile chronic polyarthritis. | | 1987 | 2822582 |
| human parvovirus infections. | discovered by chance in 1974, the human serum parvovirus b19 is at present the only recognized, autonomous, pathogenic human parvovirus. for some years following its discovery, b19 was not associated with any defined clinical syndrome; although a high titre viraemia was often noted in infected individuals they were largely asymptomatic. in 1980 the causal association between b19 infection and aplastic crisis in chronic haemolytic anaemia began to emerge with the discovery of b19 as the agent res ... | 1987 | 2822752 |
| a family outbreak of parvovirus b19 infection with petechial rash in a 7-year-old boy. | | 1987 | 2822809 |
| role of parvovirus b19 in human disease. | | 1987 | 2823211 |
| structure and mapping of the dna of human parvovirus b19. | dna from human parvovirus b19 was prepared from infected serum and examined by electron microscopy. double-stranded molecules were seen, often with characteristic 'fold-back' ends that were assumed to be due to the inverted terminal repeats of the genome dna. this double-stranded dna was mapped with 13 restriction enzymes. more than 40 isolates, including the virus from the original b19 serum, were compared. although isolates could be grouped by this method, there was no correlation between a pa ... | 1987 | 2824662 |
| [acute coxitis of the adolescent as the sole manifestation of parvovirus b19 infection]. | | 1987 | 2827148 |
| intrauterine infection with human parvovirus b19: a light and electron microscopy study. | recent reports have documented human parvovirus b19 infection in pregnancy, which may lead to spontaneous abortion, stillbirth or hydrops fetalis. transplacental infection in a case of hydrops fetalis is demonstrated by light and electron microscopy observation of virus inclusions in all tissues examined. parvovirus particles measuring approximately 20 nm were observed within the nuclei of erythropoietic cells. the parvovirus virions were observed most commonly as randomly distributed particles ... | 1988 | 2828533 |
| transient pancytopenia associated with parvovirus infection in paroxysmal nocturnal haemoglobinuria. | a 25 year old woman with a 15-year history of paroxysmal nocturnal haemoglobinuria developed transient pancytopenia following infection with human parvovirus b19. this is the first report of transient pancytopenia in a patient with an acquired haemolytic anaemia due to parvovirus. the possible mechanism of pancytopenia in such a case is discussed. | 1987 | 2829150 |
| risk of adverse outcomes of pregnancy after human parvovirus b19 infection. | human parvovirus b19 (b19) infection during pregnancy has been associated with fetal deaths. we conducted several studies to develop data needed to make recommendations for preventing fetal death associated with infection. in the first study, after an outbreak of b19 infection, specimens of cord blood from 47 infants with congenital anomalies, 10 with suspected intrauterine infection, and gestational age-matched controls were tested for igg and igm antibodies to b19. none had evidence of recent ... | 1988 | 2831283 |
| [parvovirus b19 and the fifth disease]. | | 1987 | 2831848 |
| human parvovirus b19 dna in synovial fluid. | we describe a 33-year-old woman with a serologically proven human parvovirus b19 infection, who developed synovitis. using a dot-blot hybridization technique, we detected b19 dna in her synovial fluid. to our knowledge, this is the first report of the isolation of b19 from synovial fluid. | 1988 | 2831909 |
| [parvoviruses as pathogenic agents in man]. | since 1981 an increasing number of human parvovirus infections have been reported. there is now clear evidence that parvovirus b19 is the causative agent of erythema infectiosum (fifth disease). further clinical situations associated with this virus include aplastic crisis in patients with hemolytic anemia and acute and persistent arthropathies preferentially occurring in adult women. transplacental infection during pregnancy may result in hydrops foetalis, intrauterine death and spontaneous abo ... | 1988 | 2834818 |
| human parvovirus b19-induced vesiculopustular skin eruption. | erythema infectiosum, fifth disease, is a usually benign macular or maculopapular exanthem of childhood caused by the human parvovirus b19. a 27-year-old woman with a serologically documented human parvovirus infection who presented with a hemorrhagic exanthem and enanthem with areas of pustules and pseudo-pustules is described. the histologic findings were unusual because they combined the histologic features of morbilliform and vesiculopustular viral lesions. this case serves to underscore the ... | 1988 | 2834947 |
| fetal infection with human parvovirus b19. | the case of premature infant with nonimmune hydrops secondary to human parvovirus b19 infection is presented. the infection was not suspected clinically. the fetal autopsy showed intranuclear inclusions in erythroid cells that stained positively with a dna hybridization probe for b19 parvovirus. | 1988 | 2835306 |
| human parvovirus b19 and pregnancy. | although b19 infection during pregnancy usually has no adverse effect on the fetus, it can cause fetal death. recent studies make it possible to estimate risk of infection following different types of exposure and the risk of fetal death after infection. the most common clinical manifestations of b19 infection are erythema infectiosum and aplastic crisis in patients with chronic hemolytic anemias. | 1988 | 2837357 |
| aplastic crisis caused by parvovirus b19 infection. | | 1988 | 2837393 |