| identification and mapping of neutralizing epitopes of human parvovirus b19 by using human antibodies. | we identified and mapped the regions responsible for neutralization in the human parvovirus b19 structural protein by using region-specific human antibodies derived from seropositive blood donors. the region-specific antibodies were purified by using affinity columns coupled with synthetic peptides of the hydrophilic regions including the beta-turn structure deduced by the predicted secondary structure of vp2. fifteen highly specific antibodies against the synthetic peptides were obtained. ten o ... | 1991 | 1716693 |
| detection of b19 parvovirus in human fetal tissues by electron microscopy. | we present the electron microscopy observations on samples from 38 pregnancies that were investigated for b19 parvovirus infection. thirty-four had resulted in fetal loss thought to be due to a virus infection and 22 of the 38 were positive for b19 parvovirus in one or more of the tissues. twenty-one placentas and 75 fetal tissue samples were examined. fresh samples were investigated by immune electron microscopy while formalin-fixed tissues were examined as thin sections and by negative stainin ... | 1991 | 1722505 |
| tonic pupils and human parvovirus (b19) infection. | we document the case of a young woman with bilateral internal ophthalmoplegia and subsequently tonic (adie's) pupils, corneal epitheliopathy, and uveitis due to human parvovirus b19 infection. to our knowledge, this is the first reported case of ocular complications of this virus. | 1991 | 1654342 |
| [hematopoietic failure of a healthy child with human parvovirus b19 infection--studies on the hematopoiesis]. | an 8 year-old boy, who had been healthy since his birth, presented fever, ecchymosis and nasal bleeding. his peripheral blood picture showed pancytopenia and bone marrow showed erythroblastopenia, but these findings recovered spontaneously in two weeks. the diagnosis of human parvovirus b19 (hpvb19) infection was made by the presence of anti hpvb19 igm antibodies in the sera. in order to investigate the mechanisms of erythroblastopenia and pancytopenia, we performed in vitro hematopoietic cell c ... | 1992 | 1635164 |
| production and therapeutic use of a factor xi concentrate from plasma. | factor xi deficiency is an uncommon bleeding disorder usually manifested by excessive bleeding after surgery or trauma. until recently the only effective therapy has been fresh-frozen plasma (ffp) infusion. we describe the efficacy and safety of a new factor xi concentrate produced from human donor plasma by a modification of the method used for antithrombin iii concentrate. the mean recovery of factor xi in the circulation measured on 62 occasions was approximately 91% of the injected dose, and ... | 1992 | 1641821 |
| laboratory infection with human parvovirus b19. | | 1991 | 1649224 |
| productive infection of in vitro generated haemopoietic progenitor cells from normal human adult peripheral blood with parvovirus b19: studies by morphology, immunocytochemistry, flow-cytometry and dna-hybridization. | parvovirus b19 exerts a highly selective cytopathic effect on erythroid progenitor cells. studies so far on the pathogenesis of b19-infection have been performed using bone marrow samples providing large amounts of erythroid progenitor cells. extensive study, however, has been hampered by the limited access to bone marrow samples. we have designed a liquid culture method allowing the generation of large numbers of erythroid progenitor cells, initiating cultures with cd3- and cd14-poor peripheral ... | 1991 | 1654995 |
| [diagnosis of fetal virus infections by in situ hybridization]. | non-radioactive in situ hybridization of formalin-fixed paraffin-embedded placental and foetal tissue, using virus-specific dna or rna probes, may be helpful for the diagnosis of foetal virus infection causing foetal hydrops, granulomatous placentitis and abortion. we present four cases of intrauterine cmv-, parvo-b19- and varicella-zoster virus infection, in which this diagnostic method established detection of the virus. | 1991 | 1665465 |
| chemiluminescence dot blot hybridization assay for detection of b19 parvovirus dna in human sera. | a chemiluminescence dot blot hybridization assay was used for the detection of b19 parvovirus dna in human sera by using digoxigenin-labeled probes. the probes were revealed immunoenzymatically by use of anti-digoxigenin fab fragments conjugated with alkaline phosphatase. the chemiluminescence signal was obtained by reacting the labeled probe-target complex with an enzyme-triggerable dioxetane substrate. the emitted photons were detected with instant photographic films. in the search for b19 par ... | 1991 | 1774333 |
| parvovirus b19-infected erythroblasts in fetal cord blood. | | 1991 | 1671156 |
| genetic diversity of human parvovirus b19 determined using a set of restriction endonucleases recognizing four or five base pairs and partial nucleotide sequencing: use of sequence variability in virus classification. | analysis of the restriction site polymorphism (rsp) of human parvovirus b19 using 12 restriction endonucleases (res) recognizing four or five bp sequences (4- or 5-bp res) revealed a significant difference between strains previously classified as being of the same genome type, and a relationship between two strains of different genome types, thereby indicating a global spread of b19 virus strains. these findings demonstrate the advantage of this set of 4- and 5-bp res for the calculation of the ... | 1991 | 1678779 |
| manifestations and treatment of human parvovirus b19 infection in immunocompromised patients. | we diagnosed infections from human parvovirus b19 in three patients by using dot-blot hybridization and a polymerase chain reaction to detect b19 dna and using an enzyme immunoassay to detect igg and igm to b19. for 5 months a 5-year-old boy with acute lymphoblastic leukemia in remission had anemia without reticulocytes or bone marrow erythrocyte precursors. his serum lacked igg and igm to b19 but contained b19 dna. he received gamma globulin intravenously (0.4 gm/kg/day for 5 days); his viremia ... | 1990 | 1689774 |
| pure red cell aplasia: review of treatment and proposal for a treatment strategy. | the management of pure red cell aplasia (prca) continues to challenge clinical investigators because the pathophysiology is heterogeneous and poorly understood. there are five treatment regimens that have established efficacy for patients with chronic prca. in patients with congenital hypoplastic anemia the best results have been reported using corticosteroids. cyclosporine a is recommended as the treatment of choice in acquired prca. high-dose intravenous immunoglobulin therapy is highly effect ... | 1990 | 1698487 |
| the production of human parvovirus capsid proteins in escherichia coli and their potential as diagnostic antigens. | we have expressed a number of polypeptides derived from the capsid proteins of the human parvovirus b19 in escherichia coli. these include native vp1 (84k) and vp2 (58k) proteins and also fusions to beta-galactosidase containing differing amounts of the amino terminus of the vp1/2 polypeptide. although each of these was expressed at high levels and the majority were produced as full-length proteins, only one was soluble. this soluble polypeptide, p132, is a beta-galactosidase fusion protein that ... | 1990 | 1701479 |
| [viral safety in hemotherapy. current aspects of hepatitis c and parvovirus b19 detection for plasma component therapy]. | a major risk of plasma component therapy was the transmission of infectious diseases. heat inactivation, tnbp/detergent- or beta-propiolactone/uv-treatment were introduced to reduce the risk of virus transmission, most notably those that cause hepatitis or aids. therefore we discuss topical problems of virus inactivation, particularly for the recently discovered hepatitis c-virus and the well-known parvovirus b19. | 1990 | 1703836 |
| identification of the region including the epitope for a monoclonal antibody which can neutralize human parvovirus b19. | in this study, we identified a region in the human parvovirus structural protein which involves the neutralization of the virus by a monoclonal antibody and site-specific synthetic peptides. a newly established monoclonal antibody reacted with both viral capsid proteins vp1 and vp2. the epitope was found in six strains of independently isolated human parvovirus b19. the monoclonal antibody could protect colony-forming unit erythroid in human bone marrow cell culture from injury by the virus. the ... | 1991 | 1705987 |
| [molecular biological demonstration of parvovirus infection in fetal tissue]. | we report on the morphological findings in 16 fetuses with serologically confirmed maternal parvovirus b19 infection. typical routine morphological findings of the hydropic fetuses were the presence of abnormal erythroblasts with typical nuclear inclusions. these infected cells positively stained immunohistochemically with antibodies against a recombinant virus protein, as well as they ultrastructurally contained virus particles. using in-situ hybridization techniques, we were able to demonstrat ... | 1990 | 1708624 |
| first continuous propagation of b19 parvovirus in a cell line. | the pathogenic human parvovirus b19 has extreme tropism for human erythroid progenitor cells and has resisted cultivation in conventional cell lines. we report first propagation of this virus in an erythropoietin-dependent strain of a megakaryoblastic leukemia cell line called ut-7. virus protein was present in about 5% of cells after 1 week of culture. appropriate ratios of major and minor capsid proteins were determined by immunoblot, and newly synthesized capsid protein was detected by immuno ... | 1992 | 1728307 |
| replication of parvovirus b19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood. | erythroid progenitor cells generated in vitro from peripheral human blood in the presence of interleukin-3 and erythropoietin were infected with human parvovirus b19. b19 virus dna replication was highest 48 to 72 h after infection, and maximum levels of b19 virus proteins were detected in culture supernatants at 72 to 96 h after infection. b19 virus propagated in vitro was infectious. this cell culture system with peripheral blood cells facilitates studies in vitro of b19 virus replication. | 1992 | 1731104 |
| viruses and bone marrow failure. | many agents are associated with bone marrow failure, including toxins, inherited metabolic defects, ionizing radiation, and viral infection. in most cases, the etiologic agent is unknown. many of these unclassified cases have symptomatic, immunologic, or epidemiologic similarities to viral infections. viruses from different taxonomic families have been implicated in bone marrow failure syndromes, and they appear to cause hematosuppression by a variety of mechanisms. some of the viruses involved ... | 1991 | 1655129 |
| papular-purpuric "gloves and socks" syndrome: primary infection with parvovirus b19? | | 1991 | 1655835 |
| [3 cases of kituchi's lymphadenitis in systemic lupus erythematosus. role of the parvovirus b19]. | florid necrotizing lymphadenitis, characterized by segmental infarction and lymphoid hyperplasia, is an uncommon feature of systemic lupus erythematosus (sle). kikuchi's disease is a well-defined clinicopathological entity, with a strong preference for the cervical lymph nodes of young women. the etiology of histiocytic necrotizing lymphadenitis (hnl) remains unknown, although viral agents have been proposed. hnl may reflect a self-limited sle-like autoimmune disease but full-blown sle associate ... | 1991 | 1656832 |
| human parvovirus b19 infected fetal liver as a source of antigen for a radioimmunoassay for b19 specific igm in clinical samples. | a radioimmunoassay for human parvovirus b19 igm was developed using virus antigen derived from infected fetal liver obtained post mortem. the specificity and sensitivity of this assay, compared with an established radioimmunoassay using serum antigen, was determined by testing 126 sera by both techniques. the results obtained demonstrated close concordance. false negative results were not obtained using fetal liver antigen in 58 tests on known b19 igm negative sera. sixty-four igm positive sera ... | 1991 | 1849981 |
| [hydrops fetalis caused by maternal parvovirus b19 infection]. | maternal infection with human parvovirus b19 during pregnancy is one of the rare reasons, which can lead to a not immunologically conditioned hydrops fetalis. we report here a case of intrauterine parvovirus infection associated with hydrops fetalis, diagnosed by intrauterine sonography in the 29 week of gestation, one week after the outbreak of erythema. the b19 infection of the low birth weight baby, delivered in the 30 week of gestation, was diagnosed serologically with specific igm and igg a ... | 1991 | 1850053 |
| fibromyalgia and parvovirus infection. | an infectious cause of fibromyalgia (fm) has been hypothesized based upon the observed similarity of this entity and chronic fatigue syndrome. three patients developed symptoms of fm after documented episodes of acute parvovirus b19 infections. b19 antibody determinations were obtained approximately 1 month after the symptoms began; both igm and igg titers were positive at that time. all 3 patients met criteria for fm. polysomnography performed on 2 of the patients revealed profound alpha-wave i ... | 1991 | 1657005 |
| day-care centers and the spread of cytomegalovirus and parvovirus b19. | | 1991 | 1658718 |
| [erythroblastopenia caused by parvovirus b19, without chronic hemolytic anemia, associated with iron deficiency]. | | 1991 | 1658771 |
| childhood exanthems. old and new. | exanthems are a common cause of rashes in children. several recent developments have sparked a renewed interest in this group of diseases, including the identification of parvovirus b19 and human herpesvirus 6 as the causative agents of erythema infectiosum and roseola infantum. the resurgence of measles as a childhood exanthem has caused a re-examination of current immunization practices. the most common and clinically significant exanthems are discussed, with an emphasis on new findings, origi ... | 1991 | 1870909 |
| [blood picture findings in children with parvovirus b19 infections (fifth disease/erythema infectiosum)]. | the human parvovirus b19 provokes erythema infectiosum ("e.i."); moreover there is a wide range of diseases due to parvovirus b19 without exanthema/rash. the erythropoietic blast cells of the bone marrow seems to be the main target cells for this virus. therefore in cases of prenatal infection the consequences are extremely similar to fetal erythroblastosis ("non-immunological" fetal hydrops). in postnatal life the parvovirus b19 infection causes hyporegenerative phases of the erythropoiesis wit ... | 1991 | 1660551 |
| prevalence of human parvovirus b19 in sickle cell disease and healthy controls. | the serological hpv status of 35 patients with sca (hb ss), randomly chosen from a sickle cell clinic in rural south-west togo and of 13 household members with normal haemoglobin type (hb aa) was assessed. no difference in hpv-igg seropositivity rate was found. in 30 urban hospital patients from abidjan, ivory coast, who were in acute painful sickle cell crisis hpv-igg seropositivity rate increased with age. in only one (hiv positive) patient hpv-igm antibodies were detected. it is concluded tha ... | 1991 | 1661040 |
| [transient erythroblastopenia and neutropenia revealing human parvovirus b19 infection]. | the authors report on a pediatric case of transient neutropenia with erythroblastopenia secondary to human parvovirus infection occurring in a child without underlying hemolytic disease. the heterogeneity of hematologic manifestations in such infections is discussed. | 1991 | 1662354 |
| infectious etiology of rheumatoid arthritis. | the infectious etiology of rheumatoid arthritis has been a long-standing hypothesis and in recent years is being examined with greater sophistication and scientific rigor. synovitis may result indirectly from infection by the deposition of circulating immune complexes, by molecular mimicry, by in situ antigen deposition, or by arthritogenic toxins. of candidate pathogens, recent interest has focused on mycobacterial hsp, ebv, and parvovirus b19. there is circumstantial evidence to support a link ... | 1991 | 1662820 |
| acute obstructive respiratory diseases in infants and children associated with parvovirus b19 infection. | | 1991 | 1917040 |
| human parvovirus b19 specific igg, iga, and igm antibodies and dna in serum specimens from persons with erythema infectiosum. | to determine the diagnostic use of different markers of acute parvovirus b19 infection, serum specimens obtained from 128 persons with erythema infectiosum were tested for specific immunoglobulin g (igg), iga, and igm antibodies by capture enzyme immunoassay (eia) using chinese hamster ovary (cho) cell-expressed b19 antigen, and tested for circulating b19 dna by polymerase chain reaction (pcr). a significant rise in specific igg and iga antibodies was detected in 87% and 77%, respectively, of pe ... | 1991 | 1765775 |
| [human parvovirus b19--really only fifth disease? unusual disease course in children and adolescents]. | the human parvovirus b19 agent causes infectious erythema (fifth disease). however, a wide range of other pathological manifestations may also be seen: atypical exanthema, ard (also obstructive forms, e.g. bronchiolitis), acute gastroenteritis, chronic anemia or aplastic crises (in constitutional or malignant hematological diseases or immunological deficiency), arthralgia/arthritis (e.g. rheumatoid arthritis, jca), diseases of the central nervous systems (e.g. febrile convulsions in young childr ... | 1991 | 1778531 |
| [elevated clq-bearing immune complexes in hemophiliacs with viral infections]. | one hundred hemophilia a and 30 hemophilia b patients who had been treated with non-heated and heated factor viii or prothrombin complex concentrates were examined by immunological tests including clq-bearing immune complexes assay. antibodies to human immunodeficiency virus type 1 (hiv-1), hepatitis b virus (hbv), hepatitis c virus (hcv) and human parvovirus b19 (b19) were analyzed by western blotting, enzyme immunoassay, passive hemagglutination or radio-immunoassay. clq-bearing immune complex ... | 1991 | 1779453 |
| production of digoxigenin-labelled parvovirus dna probe by pcr. | a 560-bp digoxigenin(dig)-labelled dna-probe was produced by pcr using a 699-bp parvovirus dna fragment as template with introduction of dig-dtup into the pcr reaction mixture. it was found to be very important to pay close attention to the amount of template employed, the number of cycles used, predenaturation of target dna and optimization of the percentage of dttp substituted by dig-dutp in the reaction mixture. the same 560-bp dna fragment produced by pcr without the incorporation of dig-dut ... | 1991 | 1796213 |
| [clinical variance of parvovirus-b19 infection]. | | 1991 | 1950008 |
| [human parvovirus b19 infection during pregnancy. 2 cases]. | we report two cases of non immunologic hydrops fetalis associated with intra-uterine human parvovirus b19 (pv b19) infection. the outcome was stillbirth in both cases. infection by pv b19 was suspected by the presence of intranuclear inclusions in fetal erythroblasts. it was confirmed by the presence of specific immunoglobulins m (igm) against pv b19 in maternal sera. intra-uterine infection with human pv b19 is known since 1984; this virus may cause non immunologic hydrops fetalis and stillbirt ... | 1991 | 1811010 |
| prevalence of antibodies against parvovirus b19 in norwegians with congenital coagulation factor defects treated with plasma products from small donor pools. | the seroprevalence of antibodies against parvovirus b19 in 308 norwegians with coagulation factor defects of different types and severities was assessed by an igg antibody capture radioimmunoassay (gacria). the overall seroprevalence was 62%. the seroprevalence among subjects with different types of coagulation factor defects was related to the type and severity of the coagulation factor defect: severe hemophilia a 64%, moderate and mild hemophilia a 58%, severe hemophilia b 88%, moderate and mi ... | 1991 | 1815327 |
| [maternofetal infection with human parvovirus b19. apropos of 2 cases]. | two cases of maternal-fetal human parvovirus b19 infection are reported. the first case involved a feto-placental anasarca occurring during the third trimester and complicated by in-utero death with expulsion of a fetus with multiple malformations. the second case involved meconial peritonitis during the second trimester with favourable outcome after cesarean section and resolution of the digestive syndrome. proof of pv b19 infection was obtained by identifying m specific immunoglobulins and vir ... | 1991 | 1822496 |
| parvovirus b19 infection can induce histiocytic necrotizing lymphadenitis (kikuchi's disease) associated with systemic lupus erythematosus. | we report three cases of systemic lupus erythematosus (sle) associated with necrotizing histiocytic lymphadenitis (kikuchi's disease) and immunologically proven human parvovirus b19 infection. simultaneous occurrence of sle and kikuchi's disease was a characteristic of the three cases. kikuchi's disease is an uncommon disease that usually affects young women and is characterized by painless unilateral cervical lymph-node enlargement. t-cell regions of affected lymph nodes are exclusively involve ... | 1991 | 1845362 |
| blood donor screening for parvovirus b19. | a programme of blood donor screening for parvovirus b19 was conducted from january to may 1990. the main aim of the study was to identify a b19 positive donation that could be used as a source of viral antigen for diagnostic serology. out of 24,000 donors tested one was positive for b19 antigen by counter current immunoelectrophoresis and over 100 ml of undiluted b19 containing material was obtained. however, much of the positive donation was incorporated in a plasma pool of 28 donations. an aci ... | 1990 | 1964942 |
| [human infection with parvovirus b19]. | | 1990 | 1966120 |
| parvovirus b19 infection in the fetus. | | 1990 | 1974973 |
| parvovirus b19 as the cause of a syndrome resembling lyme arthritis in adults. | | 1991 | 1846224 |
| occupational risk factors for infection with parvovirus b19 among pregnant women. | to identify exposures associated with parvovirus b19 infection during pregnancy, two groups of pregnant women were studied during an outbreak of erythema infectiosum (ei). of 796 pregnant women from connecticut who were tested serologically because of perceived exposure to b19, 53% (419/796) had serologic evidence of previous b19 infection, and 6% (23/376) of the rest had evidence of recent infection. of 121 pregnant women who had not requested testing but who lived in a community where a large ... | 1991 | 1846391 |
| detection of parvovirus by dna analysis. | dna technology has been used to diagnose parvovirus b19 infection in clinical samples. the tests are specific and can attain a high degree of sensitivity, particularly when using pcr amplification. over the next few years, dna technology will become increasingly available and may be among the routine diagnostic tests in the future. | 1991 | 1848023 |
| fetal parvovirus b19 infections. | | 1991 | 1848024 |
| recent advances in pediatric infectious diseases and their impact on dermatology. | the past five years have seen numerous advances in the field of pediatric infectious diseases, and many of these have a substantial impact on the practice of dermatology. we review some of these advances and discuss their implications on etiology, diagnosis, therapy and complications of some relatively common conditions. the etiologic agent of exanthum subitum (roseola infantum) has been clearly implicated as a herpesvirus-6. although in the classically described situation high fever in a young ... | 1991 | 2061433 |
| congenital parvovirus infection. | a case of congenital parvovirus (b19) viraemia with associated thrombocytopenic purpura and platelet antigen incompatibility in an infant is reported. results of laboratory investigations indicated that the baby was infected in utero. | 1991 | 1848056 |
| intrauterine transfusion treatment of nonimmune hydrops fetalis secondary to human parvovirus b19 infection. | congenital human parvovirus b19 infection presented in this case as nonimmune hydrops fetalis that resulted from aplastic anemia at 20 weeks' gestation. intravascular transfusion therapy led to resolution of the hydrops and a term delivery of an appropriately grown neonate. | 1991 | 1849704 |
| a cyclized peptide for studies of human parvovirus b19 infection. | synthetic peptides corresponding to human parvovirus b19 sequences were evaluated for immunoreactivity with the sera of infected persons. a cyclized peptide deduced from the n terminus of viral protein vp2 and containing the amino acids 284-307 showed a high reactivity with igm class antibodies when comparing seropositive and seronegative sera. | 1991 | 1850438 |
| human parvovirus (b19) infection. | | 1991 | 1851617 |
| predictors of the long-term outcome of early synovitis: a 5-year follow-up study. | one hundred patients who presented to an early synovitis clinic in 1979-81 were called for review after a minimum of 5 years. sufficient data for analysis were obtained in 88 cases, of whom 36 had a final diagnosis of rheumatoid arthritis (19 seropositive, 17 seronegative) and 16 human parvovirus b19 arthropathy. outcome was assessed by persistence of symptoms greater than 24 months and functional disability by health assessment questionnaire. sixty-two patients had persistent disease, with 26 s ... | 1991 | 1863820 |
| preliminary x-ray crystallographic investigation of human parvovirus b19. | crystals that diffract x rays to at least 8 a resolution have been grown from human b19 parvovirus empty capsids. these particles consist of vp-2 derived from a baculovirus expression system. this is possibly the first time that a self-assembled empty viral capsid, grown in other than normal host cells, has been crystallized. partial x-ray diffraction data have been collected using synchrotron radiation. the space group is p2(1)3 with a = 362 a. the particle position in the crystal cell is given ... | 1991 | 1871964 |
| assembly of empty capsids by using baculovirus recombinants expressing human parvovirus b19 structural proteins. | empty parvovirus b19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus b19 vp2 alone and also with a double-recombinant baculovirus expressing both vp1 and vp2. that vp2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. the stoichiometry of the capsids containing both vp1 and vp2 was similar to that previously observed in parvovirus b19-infected cells. the capsids were similar to native capsids in size ... | 1991 | 1901917 |
| spontaneous interferon-alpha antibodies in a patient with pure red cell aplasia and recurrent cutaneous carcinomas. | high-titered spontaneous interferon (ifn) antibodies were detected in a patient with pure red cell aplasia (prca), a benign mediastinal tumor, and recurrent cutaneous carcinomas. the circulating ifn antibodies reacted broadly with various human ifn-alpha subtypes (20-140 x 10(3) neutralizing units/ml serum) but not with ifn-beta or ifn-gamma, and they neutralized the antiviral activity of the patient's endogenous ifn-alpha. the ifn-alpha-binding activity was restricted to the igg1 subclass in a ... | 1991 | 1903312 |
| persistence of parvovirus b19-dna in blood of a child with severe combined immunodeficiency associated with chronic pure red cell aplasia. | in a female child with severe combined immunodeficiency, pure red cell aplasia was observed which required regular transfusions of erythrocytes. parvovirus b 19 dna (but no antibodies) was detected in stored serum samples after the death of the patient. we suggest that the anaemia was a consequence of parvovirus infection which persisted for at least 2 years due to the immunodeficiency. | 1991 | 1915497 |
| occupational risk of human parvovirus b19 infection for school and day-care personnel during an outbreak of erythema infectiosum. | human parvovirus b19, the cause of erythema infectiosum, has recently been associated with adverse fetal outcomes. during a large outbreak of erythema infectiosum in connecticut, a survey was conducted on 571 (90%) of 634 school and day-care personnel to determine the risk of acquiring b19 infection. serologic evidence of b19 infection was determined by using an enzyme-linked immunosorbent assay. of the school and day-care personnel, 58% had evidence of previous b19 infection. the minimal rate o ... | 1990 | 2157074 |
| acute parvovirus b19 infection mimicking myelodysplastic syndrome of the bone marrow. | a 36-year-old, previously healthy woman was referred to our institution with pancytopenia and splenomegaly for suspected acute leukemia. bone marrow aspiration showed marked dysplastic changes, excess of blasts, and only spurious red blood cell precursors. action was taken to prepare allogeneic bone marrow transplantation from an hla identical sibling for myelodysplastic syndrome. repeat cytological examination of the bone marrow revealed striking hyperplasia of the red cell line with presence o ... | 1992 | 1739758 |
| use of m13 single-stranded dna digoxigenin labelled probe for detection of human parvovirus b19 viraemia. | a dot-blot hybridization assay for serum b19 dna is described using a non-radioactive (digoxigenin) m13 single-stranded dna probe. the assay is sensitive (0.3 pg of b19 dna) and has several advantages over similar assays which use radioactive or non-radioactive labelled plasmid probe for the routine detection of b19 dna in sera. | 1991 | 1744217 |
| [obstructive respiratory tract diseases in children and parvovirus b19 infections]. | acute respiratory diseases (ard) due to parvovirus b 19 infection can be observed relative frequently in children. in 21 children (infants, toddlers and school children) we have seen acute or prolonged obstructive bronchitis/bronchiolitis (15 infants), acute subglottic laryngitis (3 toddlers) and acute asthmatic attacks (3 children of school age) in connection with parvovirus b 19 infection. other respiratory viruses (adeno-, influenza, parainfluenza and rs-virus) could be excluded as agents cau ... | 1991 | 1745035 |
| [human diseases caused by parvovirus b 19]. | | 1991 | 1937240 |
| removal of parvovirus b19 from contaminated factor viii during fractionation. | a solution of pooled cryo-precipitate for preparing factor viii (f viii) by the solvent/detergent method was contaminated experimentally with parvovirus b19-positive plasma to evaluate virus reduction achieved by the final steps of the f viii production process. virus reduction was at least 2 logs of the total amount of b19 virus added to the pooled cryo-precipitate. the major amount of b19 virus was detected in the solution used to regenerate the f viii-selective anion exchange chromatography c ... | 1991 | 1940880 |
| [pathomorphology of fetal parvovirus b19 infection]. | | 1991 | 1946224 |
| obstetric and neonatal infection. | recent advances in prevention, diagnosis, and treatment of infection-associated preterm labor are discussed. this includes antepartum treatment of vaginal infections, amniocentesis for culture and glucose levels, and adjunctive antibiotic treatment of preterm labor and preterm premature rupture of the membranes. risk factors for neonatal group b streptococcus sepsis are described and testing for rapid detection of maternal group b streptococcus colonization is discussed, as are recent prospectiv ... | 1991 | 1958805 |
| transfusion-transmitted diseases other than aids and hepatitis. | although the major diseases transmitted by transfusion today are aids and hepatitis, many others also are known. these include cmv, syphilis, chagas disease, babesiosis, parvovirus b19, malaria, epstein-barr infection, and many others that have been reported only once or twice. reducing the risk of transfusion-transmitted diseases is a problem for donor centers where donor screening and laboratory testing for possible carriers is undertaken. physicians should be aware that the potential for dise ... | 1990 | 1963503 |
| [infection with parvovirus b19: typical course of erythema infectiosum and its complications]. | severe chronic anemia, transitory aplastic crisis (tac), and lethal hydrops fetalis may be due to an infection with parvovirus b19 i.e. the causative agent of erythema infectiosum. 80% of the patients infected with b19 develop clinical symptoms and typically show the picture of erythema infectiosum--as one of our case reports (daughter) is going to illustrate. part of the cases, however, take a course without the characteristic slapped-face rash; here we frequently find acute arthritis and arthr ... | 1990 | 1964320 |
| prevalence of human parvovirus (b19) and rubella virus infections in urban and remote rural areas in northern brazil. | sera from inhabitants of belém, pará (542 sera), brazil and of members of 3 brazilian tribes--tiriyo/alto paru (near surinam) (212 sera), xicrin (128 sera), and mekranoiti (121 sera)--of different age and sex groups were tested for the presence of specific antibody against human parvovirus (b19) (ria) and rubella virus (latex agglutination test). parvovirus (b19) igg was found in 42.6% of the population sample from belém but in only 4.7 to 10.7% of the members of the tribes. rubella virus antibo ... | 1990 | 1964474 |
| intrauterine blood transfusion for non-immune hydrops fetalis due to parvovirus b19 infection. | | 1990 | 1975307 |
| in situ hybridization for the detection of human parvovirus b19 nucleic acid sequences in paraffin-embedded specimens. | parvovirus infection of pregnant women leading to a transplacentar infection of the fetus may result in hydrops fetalis, and ultimately in intrauterine death of the fetus. in situ hybridization with a biotinylated as well as with a 35s-labeled probe for human parvovirus b19 was performed on formalin-fixed paraffin-embedded (ffpe) tissue from a fetus suffering from non-immunologic hydrops fetalis. histology was suggestive of viral infection probably with human parvovirus. parvovirus dna could be ... | 1990 | 1980555 |
| in vitro propagation of parvovirus b19 in primary foetal liver culture. | the culture of parvovirus b19 in foetal liver tissue has been described recently. we have established the technique in our laboratory and studied parameters affecting the yield of b19 virus. replication of the virus was detected by radioimmunoassay for b19 antigen and dot blot hybridization assay of b19 dna, and the virus was localized by immunofluorescence and thin section electron microscopy. b19 dna and antigen production became detectable at day 2 and reached a maximum at day 5. virus partic ... | 1991 | 2005439 |
| analysis of splice junctions and in vitro and in vivo translation potential of the small, abundant b19 parvovirus rnas. | two parvovirus b19 cdna libraries have been constructed; one from cos-7 cells transfected with a b19/psvod hybrid vector and the other from b19-infected human erythroid leukemic cells. we have used these libraries to investigate the expression of the abundant classes of polyadenylated b19 rnas; the 700- and 800-nt class which terminates in the middle of the genome and the 500- and 600-nt class which contains an orf from the extreme right-hand end of the genome. the 700- and 800-nt rna species we ... | 1991 | 2053277 |
| detection of parvovirus b19 by dot-blot and polymerase chain reaction. | our studies compare detection of parvovirus b19 dna by dot-blot hybridization and by the polymerase chain reaction (pcr). compared to detection by dot-blot hybridization with a 32p-oligonucleotide probe, the sensitivity of pcr product detection by ethidium bromide fluorescence is not compromised when the size of the amplification target and number of cycles of amplification are properly selected. our pcr assay simultaneously amplifies two targets in the b19 genome and yields a sensitivity 10 tim ... | 1990 | 2166238 |
| parvovirus b19-induced perturbation of human megakaryocytopoiesis in vitro. | parvovirus b19 infection leads to transient aplastic crises in individuals with chronic hemolytic anemias or immunodeficiency states. an additional unexplained sequela of b19 infection is thrombocytopenia. because b19 is known to have a remarkable tropism for human erythropoietic elements, and is not known to replicate in nonerythroid cells, the etiology of this thrombocytopenia is uncertain. we sought to define the pathobiology of b19-associated thrombocytopenia by examining the role of b19 on ... | 1990 | 2146978 |
| detection of human parvovirus b19 dna by using the polymerase chain reaction. | the polymerase chain reaction (pcr) was investigated for detecting human parvovirus b19 (b19) dna in sera. three pairs of oligonucleotides were evaluated as primers. the best oligonucleotide pair spanned 699 nucleotides, including the region common to vp1 and vp2. after pcr amplification of b19 dna in serum, a 699-nucleotide dna fragment was detected on agarose gels. this dna fragment was b19 dna, because after southern transfer it hybridized to a 19-nucleotide internal probe and contained a sin ... | 1990 | 2153700 |
| an immunofluorescence assay for the detection of parvovirus b19 igg and igm antibodies based on recombinant viral antigen. | an indirect immunofluorescence assay for serum igg and igm antibodies to human parvovirus b19 was established using recombinant b19 viral antigen, the capsid protein vp1, which had been produced in a baculovirus expression system. this protein gives a strong and characteristic signal in the immunofluorescence assay, making it a suitable candidate for this test system. the test results showed a good correlation with results obtained with a solid-phase capture radioimmunoassay (cohen et al., 1983) ... | 1990 | 2170436 |
| the human parvovirus b19. | | 1990 | 2171481 |
| diagnostic of parvovirus b19 infection. | an elisa for detecting parvovirus b19 specific igm and igg was established using b19 viral particles as antigen derived from plasma of donors in the incubation phase of erythema infectiosum. at onset of erythema infectiosum anti-b19 igm was detected in titers of 10(-4) and was positive up to 19 weeks. anti-b19 igg could be detected in clinical specimens one week after onset of erythema infectiosum and persisted in titers up to 10(-6) throughout the convalescent period. among 3,289 sera of patien ... | 1990 | 2171483 |
| detection of human parvovirus b19 dna by dot-hybridization and the polymerase chain reaction: applications for diagnosis of infections. | the development of the polymerase chain reaction for the detection of human parvovirus b19 dna in clinical specimens is described. the sensitivity of this detection is compared with detection of virus using radiolabeled dna and rna probes. the polymerase chain reaction was the most sensitive, detecting approximately 10 fg b19 dna. several applications of the polymerase chain reaction for b19 dna detection are discussed: i) detection of b19 dna sequences in paraffin-embedded fetal tissues in a ca ... | 1990 | 2171484 |
| numbness and tingling of fingers associated with parvovirus b19 infection. | | 1990 | 2153742 |
| erythroid hypoplasia due to chronic infection with parvovirus b19. | | 1990 | 2154693 |
| [human parvovirus b19 and pregnancy]. | | 1990 | 2154707 |
| [fetal hydrops and parvovirus b19 infection]. | | 1990 | 2154708 |
| [human parvovirus b19 and pregnancy]. | | 1990 | 2156179 |
| use of mixture models in determining laboratory criterion for identification of seropositive individuals: application to parvovirus b19 serology. | selecting the cut-off value to identify infected individuals requires a trade-off between sensitivity and specificity. the standard technique for selecting a cut-off uses results of a population either known or assumed uninfected. using mixture models, however, it is not necessary to identify an uninfected population. this technique also provides methods to estimate the probability that an individual will be accurately classified. we illustrate this technique in determining cut-off values for th ... | 1990 | 2156877 |
| parvovirus b19 infections in pregnancy. | since 1986, 2,279 pregnant women were screened for anti parvovirus b19 igg and igm antibodies. of them 41% were seronegative, 54% had specific igg but no igm antibodies, indicating an infection in earlier life. acute infection could be demonstrated by igm antibody detection in 114 pregnant women (32% in the first, 54% in the second and 14% in the third trimester). hydrops fetalis occurred in 10 of the 114 pregnancies (8.7%). in five of these cases intrauterine exchange transfusion was done and u ... | 1990 | 2171491 |
| human parvoviruses. | human parvovirus b19 is a common infection that causes a number of clinical illnesses, most of which are benign. however, in patients with a need for increased red cell production, a deficient immune system, or both (such as occurs in the fetus), it can be a life-threatening infection. intravenous immunoglobulin should be considered in the treatment of chronic b19 infection in immunodeficient patients and possibly in the management of other serious b19 infections. recent advances in the laborato ... | 1990 | 2156940 |
| immunoglobulins in the prophylaxis of parvovirus b19 infection. | | 1990 | 2172397 |
| the genome type of human parvovirus b19 strains isolated in japan during 1981 differs from types detected in 1986 to 1987: a correlation between genome type and prevalence. | the genome dnas of 12 strains of human parvovirus b19, isolated in japan at two different times, 1981 and 1986 to 1987, were molecularly cloned in the plasmid puc18. the cloned b19 dnas were analysed by cleaving with restriction endonucleases, and were classified into several groups by genome type. the restriction endonuclease cutting patterns of b19 strains isolated during 1981 were similar to that of the group iv genome type, and the patterns of those isolated later were similar to that of gro ... | 1990 | 2157807 |
| major trends in nosocomial viral infections. | viruses have recently become appreciated as nosocomial pathogens. there is insufficient data to characterize trends in rates of viral nosocomial infections, but there have been major trends in methodologies and concepts. new groups of patients, such as infants and the elderly, are becoming appreciated as being at risk for serious nosocomial viral infections, whereas other groups, such as immunodeficient patients are expanding because of the epidemic of human immunodeficiency virus (hiv) infectio ... | 1991 | 1928151 |
| transmission of human parvovirus b19 by coagulation factor concentrates. | the prevalence of antibody to human parvovirus b19 was determined in 86 children with congenital bleeding disorders. forty-seven of 53 boys (89%) receiving non-heat-treated factor viii or prothrombin complex concentrates were anti-b19 igg positive compared with 38% of their age-matched controls and 48% of children treated with cryoprecipitate. acute b19 virus infection occurred in 2 boys 3-4 weeks after they had received the same batch of commercial factor viii concentrate. of 11 susceptible chi ... | 1990 | 2160147 |
| parvovirus infection and anaemia in a patient with aids: case report. | opportunistic infections such as pneumocystis carinii pneumonia are well-recognised in patients with the acquired immune deficiency syndrome (aids). anaemia due to a variety of causes also occurs in aids. persistent infection with parvovirus (b19) causing severe anaemia has been reported in patients with leukaemia and congenital immunodeficiency. a case is now reported of parvovirus infection and anaemia, in an adult with aids, which responded dramatically to immunoglobulin therapy. | 1990 | 2160424 |
| [parvovirus b19 infections. the cause of fifth disease-erythema infectiosum--can also cause aplastic crises, fetal damage and polyarthritis]. | human parvovirus b19 can be an important etiological factor in aplastic crises in patients with chronic hemolytic anemia, in fetal damage, and in acute polyarthritis. b19-virus can only be grown in cell cultures established from human bone marrow, where the virus production occurs in erythroblasts. parvovirus can cause severe, often fatal, infections in various kinds of animals, also in their fetuses, and many cases of teratogenic changes have been described. the b19-infection is diagnosed by de ... | 1990 | 2160748 |
| [the fifth disease--erythema infectiosum. parvovirus infection in a family group]. | the causative agent of erythema infectiosum (ei), also known as the fifth disease, has been shown to be human parvovirus b19. this virus may also cause foetal damage, resulting in spontaneous abortion, hydrops foetalis or stillbirth. three cases of human parvovirus infection in a family are presented. the child has a classical rash. the father had a rash and joint-symptoms, which is common in adults. the mother had a subclinical infection, which is seen in the majority of ei cases. as the woman ... | 1990 | 2160749 |
| prospective study of human parvovirus (b19) infection in pregnancy. public health laboratory service working party on fifth disease. | to determine the fetal infection rate and outcome of pregnancy among women who acquire infection with human parvovirus (b19) in the antenatal period. | 1990 | 2161263 |
| red cell aplasia associated with human parvovirus b19 and hiv infection: failure to respond clinically to intravenous. | | 1990 | 2175617 |
| b19 chronic bone marrow failure: a persistent parvovirus infection of humans. | the human parvovirus b19 may produce persistent infections with chronic bone marrow failure in immunocompromised hosts. despite poor data on the pathophysiologic basis of such infections, it is likely that immune humoral response plays an important part. indeed, administration of immunoglobulin preparation may help to control the course of b19 chronic infections. | 1990 | 2161513 |
| fifth disease: role for nurses in pediatric practice. | parvovirus b19 infection is a common childhood disease. infection is usually mild but may have serious consequences in pregnant women. nurses in pediatric practice may be the only health professionals in a position to recognize and intervene in this potentially serious condition in certain high risk individuals. | 1990 | 2163055 |