Publications

the complete nucleotide sequence of pea seed-borne mosaic virus rna.	the complete nucleotide sequence of the rna genome of pea seed-borne mosaic virus (psbmv) was determined from cloned cdna and by direct sequencing of viral rna. the psbmv genomic sequence was determined to be 9924 nucleotides in length excluding the poly(a) tract. the rna contained an open reading frame (orf) of 9618 nucleotides with the potential to encode a polyprotein with a calculated mr of 364000 (364k). the orf was flanked by a 5' untranslated leader sequence of 143 nucleotides and a 3' un ...	1991	1940858
identification of the initiation codon of plum pox potyvirus genomic rna.	the expression of plum pox potyvirus (ppv) genomic rna takes place through translation of its unique long and functional open reading frame (orf) into a large polyprotein that undergoes extensive proteolytic processing. in this paper we show that the aug recognized as the initiation codon of the ppv orf by in vitro translation systems is the one found at nucleotide position 147, in spite of the presence at position 36 of an in-phase aug that marks the start of the orf. deletion of a substantial ...	1991	1962436
a current assessment of the role of porcine parvovirus as a cause of fetal porcine death.	one hundred one litters containing 1 or more dead porcine fetuses were collected at an iowa abattoir during a 2-month interval and examined for evidence of viral infection. each of 1,137 fetuses (302 dead, 835 alive) of these litters was tested for porcine parvovirus (ppv) antigens by direct immunofluorescence microscopy (fa) of fetal lung. antigens of ppv were detected in the lungs of most of the fetuses of 11 of the litters. the 11 fa-positive litters contained 105 dead (100 fa-positive) and 1 ...	1991	1645596
experimental in utero infection of pig foetuses with porcine parvovirus (ppv).	pig foetuses of various gestational ages were exposed to experimental infection with porcine parvovirus (ppv) in utero. inoculation of 40-, 50- and 60-day-old foetuses with ppv caused foetal death and mummification and spread of the infection to non-inoculated foetuses. inoculation at 80 and 100 days gestation caused pathological lesions of various degrees whereas spread of infection occurred only sporadically. serological examinations of foetuses of different ages suggest that immunocompetence ...	1991	1653481
comparison of two rapid methods for detection of respiratory syncytial virus (rsv) (testpack rsv and ortho rsv elisa) with direct immunofluorescence and virus isolation for the diagnosis of pediatric rsv infection.	the ability of two commercial immunoassays to detect respiratory syncytial virus (rsv) in respiratory specimens was evaluated as follows: 152 specimens were tested by testpack rsv (abbott), and 72 were tested by ortho rsv elisa (ortho). test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (dfa) alone, or virus isolation and/or dfa. testpack rsv versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (ppv), and 95% ...	1991	2037684
nucleotide sequence of the 3'-terminal region of potato virus a rna.	the sequence of the 3'-terminal region of the genome of the potato virus a (pva) was obtained from two independent cdna clones. this sequence is 1383 nucleotides long and contains an open reading frame of 1178 nucleotides, ending with the translation termination codon taa and followed by untranslated region of 205 nucleotides. since the n-terminal amino acid of the coat protein of pva was blocked, the position of the putative coat protein cleavage site has been deduced by searching for consensus ...	1992	1604933
restriction of porcine parvovirus replication in nonpermissive cells.	swine testicle (st) cells and madin-darby canine kidney (mdck) cells differ in their ability to support replication of porcine parvovirus (ppv). viral replication events in st cells, a permissive cell type, and mdck cells, a nonpermissive cell type, were compared in an attempt to elucidate putative mechanisms of restrictive virus replication. radiolabeled ppv bound to the cell surface of both cell types equally well and the binding was shown to be ppv specific, indicating that the restriction wa ...	1992	1370555
construction of a chimeric viral gene expressing plum pox virus coat protein.	the capsid-encoding gene of plum pox virus (ppv) was fused with the leader sequence of the coat protein mrna (cp) of tobacco mosaic virus by a novel mutagenesis technique which involves reverse transcription of minus-strand rna [synthesized by in vitro transcription of a double-stranded (ds) cdna clone], using an ad hoc synthetic oligodeoxynucleotide as primer. the resulting cdna was rendered ds and cloned into the plasmid, pbluescribe m13+. transcription of this chimeric construction produced r ...	1992	1398133
a monoclonal antibody which recognizes cell surface antigen and inhibits porcine parvovirus replication.	monoclonal antibody technologies were applied to the study of early events in porcine parvovirus (ppv) infections in vitro. balb/c mice were immunized with whole swine testicle cells and hybridomas were produced following fusion with myeloma cells. resultant clones were screened firstly in an elisa system, to detect monoclonal antibody recognition of swine testicle cells, and secondly, in a fluorescent antibody test to detect monoclonal antibody which inhibited production of ppv antigen. one clo ...	1992	1562235
an enzyme-linked immunosorbent assay for detection of porcine parvovirus in fetal tissues.	an enzyme-linked immunosorbent assay for porcine parvovirus was developed for laboratory detection of parvovirus antigen in fetal tissues and compared with the fluorescent antibody and haemagglutination tests. the elisa proved to be superior in terms of specificity, sensitivity, speed of performance and ease of interpretation.	1992	1331147
proteolytic processing of the plum pox potyvirus polyprotein by the nia protease at a novel cleavage site.	the expression of potyvirus genomic rna takes place through translation of its unique long and functional open reading frame into a large polyprotein that undergoes extensive proteolytic processing. most of the cleavages are performed by the virus-encoded nia protease, which cuts the polyprotein at defined sites that are characterized by conserved heptapeptide sequences. we have demonstrated in vitro cleavage activity by the plum pox potyvirus (ppv) nia protease at a novel site, previously ident ...	1992	1585641
antigenic variant of swine influenza virus causing proliferative and necrotizing pneumonia in pigs.	a new antigenic variant of swine influenza virus was isolated from the lungs of pigs experiencing respiratory problems in 7 different swine herds in quebec. pigs of different ages were affected, and the main clinical signs were fever, dyspnea, and abdominal respiration. coughing was not a constant finding of the syndrome. at necropsy, macroscopic lesions included the overall appearance of pale animals, general lymphadenopathy, hepatic congestion, and consolidation of the lungs. histopathologic f ...	1992	1333815
antibodies to some swine diseases in commercial piggeries in central zambia.	blood samples were taken from 121 sows and gilts on 7 commercial piggeries located around lusaka (zambia). the samples tested negative for antibodies to aujeszky's disease, transmissible gastroenteritis (tge), swine influenza, hog cholera and brucellosis. seventy-eight pigs from 5 farms had positive titres to porcine parvovirus. eighteen sera showed positive titres to leptospira celledoni.	1992	1339985
reproductive failure associated with porcine parvovirus in an enzootically infected pig herd.	two outbreaks of porcine parvovirus occurred in a unit of 380 sows, with a subsequent decrease in the size of gilts' litters. of the 203 gilts and 64 primiparous sows which were seronegative at the time of insemination, 134 gilts and 55 primiparous sows seroconverted during pregnancy. of the second parity sows nine of 271 were still seronegative at the time of insemination but all nine seroconverted during their third gestation. the gilts that seroconverted during their first pregnancy produced ...	1992	1338661
the complete nucleotide sequence of pepper mottle virus genomic rna: comparison of the encoded polyprotein with those of other sequenced potyviruses.	the complete nucleotide sequence of a pepper mottle virus isolate from california (pepmov c) has been determined from cloned viral cdnas. the pepmov c genomic rna is 9640 nucleotides excluding the poly(a) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. comparison of the pepmov c presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (tev), tobacco vein mo ...	1992	1413501
stillbirths, mummies, abortions, and early embryonic death.	stillbirths, mummies, abortions, and early embryonic death have a substantial impact on the profitability of a farm in both endemic and epidemic conditions. fetal death is highly dependent on stage of gestation. implantation occurs around day 14 postmating in sows, and fetal death of an entire litter at this time usually results in a regular return to service. if more than four embryos remain alive, the sow may go on to farrow normally. if fetal death occurs after implantation but before calcifi ...	1992	1446274
production of porcine parvovirus empty capsids with high immunogenic activity.	the vp2 gene of porcine parvovirus was cloned in the baculovirus system and expressed in insect cells. the resulting product was present in high yield. it self-assembled into particles which were structurally and antigenically indistinguishable from regular ppv capsids. a high degree of purity of the recombinant capsids was obtained by ammonium sulphate precipitation of cell lysates. these virus-like particles were used as antigen in the immunization of two pigs. the pigs elicited an immune resp ...	1992	1523879
the ns and capsid genes determine the host range of porcine parvovirus.	porcine parvovirus is an autonomous parvovirus which normally infects pigs and multiplies in porcine cells in vitro. in this report, we describe the properties of a variant designated p2, which has extended its host range to include canine cells. the variant was able to produce cytopathic effects (cpe) in canine cells, unlike the prototype nadl-2 strain. the variant also produced higher viral antigen and infectivity titers in canine cells than the nadl-2 strain, whereas both strains produced cpe ...	1992	1532105
infectious in vivo transcripts of a plum pox potyvirus full-length cdna clone containing the cauliflower mosaic virus 35s rna promoter.	a full-length cdna clone of an aphid non-transmissible isolate of plum pox potyvirus (ppv) was rendered biologically active when placed under the control of the cauliflower mosaic virus 35s rna promoter and the nopaline synthase polyadenylation signal. the cdna was constructed so that the exact 5' end of the ppv rna was present at the transcription initiation site. inoculation of plasmid dna onto nicotiana benthamiana led to systemic infection, whereas local lesions were produced in chenopodium ...	1992	1545225
the response of pregnant gilts previously given an inactivated preparation of porcine parvovirus (ppv) to challenge infection with a fully virulent ppv.	eight 40-day pregnant gilts, previously treated with an adjuvanted-inactivated viral preparation (aivp) obtained with a field strain of porcine parvovirus (ppv) together with 4 pregnant untreated controls, were subjected to challenge infection with a virulent strain of ppv at the 40th day of gestation. after challenge, all controls became febrile for 2 to 8 days, whereas only one gilt among those which had been treated with the aivp experienced fever which lasted 4 days. virus was consistently r ...	1992	1331715
field trials of an inactivated virus vaccine against porcine parvovirus.	serological response and reproductive performance were estimated in field trials of an inactivated virus vaccine against porcine parvovirus. experiments were carried out in 10 selected pig breeding herds. a total of 277 seronegative gilts were used. two hundred and twenty animals were vaccinated twice before mating, fourteen days apart and revaccinated after farrowing. blood samples were obtained from both vaccinated and non-vaccinated (57 animal) control gilts, one week after the 2nd dose of va ...	1992	1325724
immunohistological diagnosis of rabbit haemorrhagic disease (rhd).	in the present study the diagnostic use of a biotinylated serum from an immune rabbit was investigated by means of an avidin-biotin-complex (abc)-peroxidase method on paraffin sections. 15 cases of rhd which had been verified histologically and/or by haemagglutination test (ha), 4 suspected cases and 3 cases without history of rhd were included (cases 1 to 22). from 5 prospective cases a wider tissue range was examined (cases 23 to 25 and 29 to 30). furthermore lungs, liver and placenta of 3 fet ...	1992	1325718
comparison of the virulence of two isolates of porcine parvovirus in 72-day-old porcine fetuses.	two strains of porcine parvovirus (ppv), designated kresse and nadl-8, were compared for relative virulence in porcine fetuses. strain kresse was injected into the amniotic fluid of all fetuses of 1 uterine horn of each of 2 pregnant gilts at 72 days of gestation. strain nadl-8 was administered similarly to fetuses of 4 other gilts at the same stage of gestation. all gilts were killed and necropsied 35 days later. selected tissues of all fetuses were tested for infectious virus and viral antigen ...	1992	1325191
a delphi exercise used to identify potential causes of variation in litter size of ontario swine.	forty-eight people, considered to the swine experts, were asked to collaborate in a delphi exercise to identify the factors which they believed affect litter size in ontario swine. the panel included 16 animal scientists, 16 pork producers, and 16 veterinarians in swine practice. the ten factors with the highest ratings were parity of the sow, mycotoxins in the feed, infections with porcine parvovirus or leptospira spp., breeding gilts on their second versus first observed estrus, the timing of ...	1992	17423928
[return to estrus following first insemination in sow herds (incidence and association with reproductivity and various blood parameters)].	as no systematic study has been done to get an accurate estimate of the incidence of return to oestrus after first insemination in sows in the netherlands, the objectives of this investigation were: 1) to obtain an estimate of the incidence of return to oestrus after insemination at the herd level; 2) to investigate the association between incidence of return to oestrus after first insemination and reproduction characteristics to get an impression of the economic importance. these objectives wer ...	1993	7505958
[the infectious causes of abortion and stillbirth in swine in switzerland].	fetuses and placentae of 171 cases of porcine abortion, stillbirth and mummification were examined for pathological lesions, bacterial infections and ppv (porcine parvovirus) infection. furthermore igg (immunoglobulin g) levels were determined in fetal body fluids. selected maternal sera were tested for antibodies against leptospira, aujeszky's disease and hog cholera. ppv infection was diagnosed in 29.2% of all cases. bacterial abortion was diagnosed in 8.2%. indications for an infectious agent ...	1993	8128797
genomic organization and mapping of transcription and translation products of the nadl-2 strain of porcine parvovirus.	the nadl-2 strain of ppv was cloned into puc19 and independent infectious clones were sequenced. this permitted a correction of published sequences and to predict a cruciform structure as an alternative to the 5'-hairpin of the "-" strand. this 5'-end structural covariance is shared with other parvoviruses of the same group and two alternative sequences ("flip" and "flop") were present in the region of the cruciform. transcript and translation product mapping allowed the prediction of the locati ...	1993	8212598
a new method for rapidly removing contaminating micro-organisms from porcine parvovirus or pseudorabies virus master-seed suspensions.	virus-contaminated cell cultures are a major problem in the bio-industry. methods employed to date to remove contaminating micro-organisms are slow and costly, and a new method is proposed here which is simple and rapid. the method uses polyacrylamide beads coated with specific antibodies which yielded bead-antibody-virus complexes when suspended in the virus solution to be cleared. the purified virus was propagated in cells which show phagocytic activity. vaccine master-seed virus is shown to b ...	1993	8383386
role of the tk+ phenotype in the stability of pigeonpox virus recombinant.	insertion of foreign dna containing the e. coli gpt marker by homologous recombination in the pigeonpox virus (ppv) thymidine kinase (tk) gene and selection for the presence of this dna in the viral genome produced unstable recombinants after 3 plaque purifications. we highlight the persistence of duplicated tk dna sequences arising from single crossing over, due to the growth advantage of tk+ virus. restoration of the tk function by coinsertion of the vaccinia virus tk gene led to stable tk+ re ...	1993	8394070
an economic assessment of porcine parvovirus vaccination.	a decision analysis model was designed to evaluate the cost effectiveness of a vaccination program for preventing endemic or epidemic porcine parvovirus (ppv) induced reproductive failure in a 100-sow pig herd. the results showed that the cost of vaccination was less than the cost incurred by continuing endemic ppv infection, or the cost of a severe epidemic. a long term vaccination program is a cost effective method for controlling ppv-induced reproductive failure in pig herds suffering endemic ...	1993	8393655
[suppression of replication of swine parvoviral antisense rna against the ns ppv gene in swine thyroid gland cells].	the possibility of suppression of porcine parvovirus (ppv) reproduction in the culture of thyroid gland cells of a swine that contain the integrated genes for asrna against the nonstructural proteins of the virus has been studied. 10 cell lines with the asrna genes have been obtained. the line with the maximal number of integrated gene copies was used to inflict with the parvovirus. the expression of asrna in this cell line was shown to lead to 95% suppression of ppv replication as compared with ...	1993	8510680
comparative sequence analysis of four complete primary structures of plum pox virus strains.	the complete nucleotide sequence of plum pox virus (ppv) strain sk 68 was determined from a series of overlapping cdna clones. the exact 5' terminus was determined by direct rna sequencing. the rna sequence was 9786 nucleotides in length, excluding a 3' terminal poly(a) sequence. the large open reading frame starts at nucleotide position 147 and is terminated at position 9568. comparison of cistrons from other plum pox virus strains with those predicted for the sk 68 strain indicated the same ge ...	1993	8122394
monoclonal antibodies suitable for plum pox virus determination.	monoclonal antibodies (mabs) to plum pox virus (ppv) were obtained after immunization of balb/c mice with purified ppv-w isolate. spleen cells from a mouse showing a high serum titer were used for fusion with sp2/0-ag14 myeloma cells. culture supernatants were screened for specific antibody production against ppv-w isolate using indirect elisa. a total of six stable hybridoma lines producing mabs of igg class were obtained. all four ppv isolates tested (w, a, d and m) can be distinguished by the ...	1993	8314598
tissue tropisms of porcine parvovirus in swine.	late-term gestation swine fetuses, similar to adult animals, are able to effectively mount immune response and survive porcine parvovirus (ppv) infection. an exception to this is the kresse strain of ppv, which causes fetal death in late-term gestation swine fetuses. in an effort to understand the basis for this profound difference in pathogenicity between kresse strain and the prototype strain of ppv, nadl-8, studies were designed to examine potential difference in sites of replication and quan ...	1993	8390826
evaluation of testing algorithms following the use of combination hiv-1/hiv-2 eia for screening purposes.	the licensure of combination human immunodeficiency virus type 1 and type 2 (hiv-1/hiv-2) enzyme immunoassays (eias) by the food and drug administration has been accompanied by a recommendation that u.s. blood banks begin testing the nation's blood supply for hiv-2 by june 1, 1992. the performance of a recently licensed combination hiv-1/hiv-2 eia (genetic systems) was evaluated using 3100 sera collected in the united states. a total of 2,049 sera were obtained from populations with low risk for ...	1993	8457381
immunodetection of the plum pox virus helper component in infected plants and expression of its gene in transgenic plants.	tobacco plants (nicotiana tabacum cv. xanthi) have been transformed via agrobacterium tumefaciens vectors, with cdnas corresponding to the plum pox virus (ppv) cistron 2 encoding helper component (hc-pro) and with the first two and half cistrons of the ppv genome. presence of the hc-pro in ppv-infected plants and transgenic plants transformed with the gene coding for this protein was investigated using specific polyclonal antibodies produced against the ppv hc-pro. the results suggest that two p ...	1993	8517789
3'-terminal sequence of the plum pox virus ps and ŏ6 isolates: evidence for rna recombination within the potyvirus group.	the sequence of the 3'-terminal 1768 nucleotides of the ps and ŏ6 isolates of plum pox virus (ppv) has been determined and compared with that of the equivalent regions of other ppv isolates sequenced previously. the sequenced region is part of the ppv open reading frame encoding the last 186 amino acids of the nib protein and the coat protein (cp, 330 amino acids), followed by a non-coding region of 220 nucleotides and a poly(a) tail. ppv-ps and ppv(-)ŏ6, just like ppv-el amar, show rather high ...	1993	8445362
a serologic survey of selected viral and bacterial diseases of european wild hogs, great smoky mountains national park, usa.	blood samples were collected from 108 wild hogs (sus scrofa) from the great smoky mountains national park (gsmnp), usa, february to july 1990. we found no antibodies for swine brucellosis, pseudorabies, bovine virus diarrhea virus or porcine rotavirus infection. antibody titers to porcine parvovirus were found in 15 (14%) samples and antibody to one or more leptospiral serovars was found in 48 (44%) samples. thirty-nine (89%) of the 44 positive samples reacted to all five leptospiral serovars te ...	1994	8151810
nucleotide sequence of the coat protein gene of the skierniewice isolate of plum pox virus (ppv).	the coat protein (cp) gene of the skierniewice isolate of plum pox virus (ppv-s) has been amplified using the reverse transcription--polymerase chain reaction (rt-pcr), cloned and sequenced. the nucleotide sequence of the gene and the deduced amino-acid sequence of ppv-s cp were compared with those of other ppv strains. the nucleotide sequence showed very high homology to most of the published sequences. the motif: asp-ala-gly (dag), important for the aphid transmissibility, was present in the a ...	1994	7913278
porcine parvovirus associated with cutaneous lesions in piglets.		1994	7948207
sensitive non-radioactive nucleic acid hybridization assay for plum pox virus detection.	a new non-radioactive sandwich hybridization assay was designed to simplify the analysis of a large number of plant samples. plant material was homogenized in 0.5% sds and added directly to the hybridization reaction, in which a pair of identifying probes were used. one of the probes was biotinylated capture rna specific for plum pox virus (ppv) strain sk-68; the other rna probe was synthesized from a plasmid bearing the adjacent sequence of this strain and was labelled with digoxigenin (dig). b ...	1994	7709075
production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of mediterranean isolates.	monoclonal antibodies (mabs) specific to plum pox virus (ppv) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (cp). the characterized mabs could be used in elisa to differentiate several mediterranean ppv isolates differing in their geographical origin and cp size. at least seven antigenic sites could be established based on the recognition pattern and ...	1994	7526822
use of polymerase chain reaction to detect porcine parvovirus associated with swine embryos.	the role of porcine parvovirus (ppv) in inducing reproductive failure in swine has been extensively documented. however, information is not available as to the risk of ppv transmission by embryo transfer. using the polymerase chain reaction (pcr) technique, ppv-specific dna was detected in association with 4-day-old porcine embryos incubated in vitro in the presence of nadl-8 strain of ppv, despite attempts to rid the embryos of virus by either washing or treatment with pronase or trypsin. the p ...	1994	8192255
return to oestrus after first insemination in sow herds (incidence, seasonality, and association with reproductivity and some blood parameters).	as no systematic study has been done to get an accurate estimate of the incidence of return to oestrus after first insemination in sows in the netherlands, the objectives of this investigation were: 1) to obtain an estimate of the incidence of return to oestrus after insemination at the herd level; 2) to investigate the association between incidence of return to oestrus after first insemination and reproduction characteristics in order to get an impression of the economic importance of reproduct ...	1994	7985351
a novel non-mineral oil-based adjuvant. ii. efficacy of a synthetic sulfolipopolysaccharide in a squalane-in-water emulsion in pigs.	the adjuvanticity of a sulfolipopolysaccharide (slp) incorporated into a squalane-in-water emulsion (slp/s/w) was compared with that of a mineral oil-in-water (o/w) adjuvant currently used in commercial porcine vaccines. groups of pigs were immunized twice with vaccines comprising either inactivated influenza virus (iflu3 containing strains a/swine, mrc-11 and x-79), inactivated pseudorabies virus (iprv), live pseudorabies virus (prv) or inactivated porcine parvovirus (ippv) as antigen and slp/s ...	1994	8085386
[recent developments in the diagnosis of parapoxviruses].	neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (ppv) were generated by immunizing balb/c-mice with gradient-purified ppv orf d-1701 or purified envelopes. epitope specificity studies identified three distinct epitopes localized in the virus envelope. these antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. for a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, ...	1994	7519370
rapid immunoassay for the detection of genital herpes infection.	we evaluated a new affinity membrane strip test for the diagnosis of herpetic genital infections. test strip results, which are available by immunoassay in 30 min without the need for special equipment, were compared with the results of viral culture.	1994	18475363
adventitious pestivirus rna in live virus vaccines against bovine and swine diseases.	live virus vaccines against bovine and porcine diseases were examined for the presence of adventitious pestivirus rna or pestiviruses by reverse transcription-polymerase chain reaction (pcr). pestivirus rna was detected in the live virus vaccines against akabane disease, ibaraki disease, infectious bovine rhinotracheitis, porcine parvovirus infection, transmissible gastroenteritis and japanese encephalitis. pestivirus rna or pestivirus in the fetal bovine serum used to grow the host cells used t ...	1995	7762264
interferon induction in peripheral blood mononuclear leukocytes of man and farm animals by poxvirus vector candidates and some poxvirus constructs.	prototypes of three poxvirus genera--orthopoxvirus (opv), parapoxvirus (ppv), avipoxvirus (apv)--and newcastle disease virus (ndv) as a control, as well as three recombinant opv strains and one recombinant apv strain, were incubated in vitro with peripheral blood mononuclear leukocytes (pbml) of man, sheep and swine. antiviral activity was determined in pbml culture supernatants at different time intervals after virus cell interaction using a cytopathic effect inhibition bioassay. additionally, ...	1995	7502485
virucidal short wavelength ultraviolet light treatment of plasma and factor viii concentrate: protection of proteins by antioxidants.	the use of solvent/detergent mixtures and various forms of heat treatment to inactivate viruses has become widespread in the preparation of blood derivatives. because viruses that lack lipid envelopes and/or are heat resistant, eg, hepatitis a virus (hav) or parvovirus b19 may be present, the use of two methods of virus elimination that operate by different mechanisms has been advocated. we now report on short wavelength ultraviolet light (uvc) irradiation for virus inactivation and enhancement ...	1995	7492794
properties of the active plum pox potyvirus rna polymerase complex in defined glycerol gradient fractions.	as a first step in the study of the replication of plum pox virus (ppv) rna, an in vitro virus-specific rna polymerase activity was characterized in a crude membrane extract (martin and garcia, 1991). in this study, we report the fractionation of the crude membrane extract by centrifugation in glycerol gradients. the sedimentation properties after different treatments of the crude extract and its insensitivity to micrococcal nuclease treatment suggest that the rna polymerase activity was localiz ...	1995	7483826
expression of the plum pox coat protein gene in transgenic nicotiana tabacum plants.	plant expression vector pbi 121 containing the gene encoding coat protein of plum pox virus of the skierniewice isolate (cp ppv-s) was prepared (clone pcm1). the construct was used for transformation of nicotiana tabacum plants using an agrobacterium tumefaciens based system. about 82% of kanamycin resistant plant lines contained a transgene (the sequence of cp ppv-s) but only 81% of them actively expressed the ppv-s coat protein gene as measured by rt-pcr.	1995	7653168
rna helicase activity of the plum pox potyvirus ci protein expressed in escherichia coli. mapping of an rna binding domain.	the plum pox potyvirus (ppv) cylindrical inclusion (ci) protein fused to the maltose binding protein (mbp) has been synthesized in escherichia coli and purified by affinity chromatography in amylose resin. in the absence of any other viral factors, the fusion product had ntpase, rna binding and rna helicase activities. these in vitro activities were not affected by removal of the last 103 amino acids of the ci protein. however, other deletions in the c-terminal part of the protein, although leav ...	1995	7538661
immunogenicity of poliovirus b and t cell epitopes presented by hybrid porcine parvovirus particles.	we have analysed the potential capacity of hybrid porcine parvovirus (ppv) capsids to present foreign epitopes to the immune system. foreign sequences were introduced into the n and c termini of ppv vp2, which was previously shown to assemble spontaneously into parvovirus-like particles. the integrity of the c terminus was shown to be essential for preserving the structure of the capsid and therefore could not be used for epitope fusion. in contrast, insertion of sequences corresponding to t and ...	1995	7561778
transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus y helper component.	two spanish plum pox virus (ppv) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector myzus persicae, with woody and herbaceous host plants. these isolates differ in the size of their coat protein (cp) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the n terminus of the cp, affecting the same positions as in a previously reported non-aphid-transmissible ppv isolate from germany. aphid transmission experiments showed that isol ...	1995	7561767
age-specific prevalence of porcine parvovirus antibody in feral pigs from a site in the northern territory.		1995	8787527
detection of respiratory syncytial virus by reverse transcription-pcr and hybridization with a dna enzyme immunoassay.	nasal aspirates from 238 infants hospitalized with acute respiratory infections during the winter of 1994 and 1995 were tested for respiratory syncytial virus (rsv) by immunofluorescence assay (ifa) and the viral isolation technique (vit) and by two pcr and hybridization methods: reverse transcription pcr 1 (rt-pcr1), which amplifies the rnas of all rsv strains, and rt-pcr-2, which allows subgroup classification of rsv. rt-pcr-1 and rt-pcr-2 detected viral sequences in 56.7% (135 of 238) and 48. ...	1995	8586738
processing of the plum pox virus polyprotein at the p3-6k1 junction is not required for virus viability.	proteolytic processing of the potyvirus polyprotein is mainly performed by the virus-encoded nia protease, whose cleavage sites are characterized by conserved heptapeptide sequences. partial processing at the cleavage site present between the p3 and 6k1 cistrons by the plum pox potyvirus (ppv) nia protease has been previously shown to occur in vitro. we have now studied the role of polyprotein processing at the p3-6k1 junction in vivo, using a full-length ppv cdna clone. ppv mutant transcripts c ...	1995	9049341
acute outbreak of porcine parvovirus infection in mozambique.	investigations were made to determine the causal agent of an acute outbreak of abortions recorded in a swine herd in mozambique. isolation of porcine parvovirus and demonstration of its specific antibodies accomplished by using enzyme-linked immunosorbent assay, haemagglutination inhibition and immunofluorescent tests, indicated that porcine parvovirus was the causal agent of the abortions. other pathogenic agents causing reproductive failure, e.g. pseudorabies virus, leptospira or brucella spec ...	1995	8966762
localization of fruit tree viruses by immuno-tissue printing in infected shoots of malus sp. and prunus sp.	immuno-tissue printing protocols for the localization of apple chlorotic leaf spot virus (aclsv), stem grooving virus (sgv) and plum pox virus (ppv) in shoots of prunus and malus in vitro have been established for routine diagnosis in a virus elimination program. since these viruses belong to different virus genera, the protocols were adapted according to the properties of the virus under investigation. accumulation of aclsv was highest in the base of the stem and decreased towards the apex of t ...	1995	8537455
diagnosis of fetal infection with porcine parvovirus by in situ hybridization.	in situ hybridization (ish) for the diagnosis of fetal infection with porcine parvovirus (ppv) was compared with immune electron microscopy (iem) and serology by immunofluorescence (if) for its sensitivity and its applicability in a routine diagnostic laboratory. the technique was applied to the examination of sections of formalin-fixed paraffin-embedded tissues from 68 fetuses. fifty-three of these fetuses were diagnosed serologically since they had a crown rump length of more than 17 cm, i.e. ...	1995	8748552
inactivation of virus during anaerobic digestion of manure in laboratory scale biogas reactors.	reduction of porcine parvovirus, bovine enterovirus and faecal enterococci were measured in biogas reactors continuously run on manure and manure supplemented with household waste at 35 degrees c and 55 degrees c and in batch test run at 70 degrees c. the aim of the experiments was to study the sanitation effect of anaerobic digestion and to evaluate the use of faecal enterococci as an indicator of sanitation. parallel studies on the reduction of virus and faecal enterococci were done in physiol ...	1996	8678476
first findings of plum pox virus in walnut trees (juglans regia l.)	plum pox virus (ppv) was transmitted from infected buds and leaves of walnut tree (juglans regia l.) on the following herbaceous indicators: chenopodium foetidum schrad., nicotiana bigelovii var. quadrivalvis fuchs., n. clevelandii x n. glutinosa. a positive elisa reaction with antisera against ppv was obtained from infected buds and leaves of juglans regia l. and from attacked leaves of indicator plants.	1996	8886101
comparison of the safety and immunogenicity of a pneumococcal conjugate with a licensed polysaccharide vaccine in human immunodeficiency virus and non-human immunodeficiency virus-infected children.	to compare the safety and immunogenicity of a 5-valent pneumococcal conjugate vaccine to a licensed 23-valent polysaccharide pneumococcal vaccine in hiv-infected and non-hiv-infected children > or = 2 years old.	1996	8852905
financial evaluation of vaccination and testing alternatives for control of parvovirus-induced reproductive failure in swine.	to identify the preferable testing and vaccination strategy for control of porcine parvovirus (ppv) during a 6-month period.	1996	8617643
genome organization of the kresse strain of porcine parvovirus: identification of the allotropic determinant and comparison with those of nadl-2 and field isolates.	the kresse strain of porcine parvovirus (ppv) was cloned into puc19, and independent infectious clones were sequenced. the ppv kresse and nadl-2 strains, which have different pathogenicities, shared an identical genomic organization and a high degree of sequence identity. partial genomes (1.5 or 1.6 kb) of 15 field isolates were also amplified by pcr in regions with significant sequence differences between the laboratory strains. five amino acid differences were consistently present within the v ...	1996	8642680
the rna helicase ci from plum pox potyvirus has two regions involved in binding to rna.	the plum pox virus (ppv) protein ci is an rna helicase, whose function in the virus replication is still unknown. recently, an rna binding domain was mapped to a region of the ci protein that includes the arginine-rich motif vi typical of rna helicases of the superfamily sf2. in the present study, a second region involved in rna binding activity of the ci protein has been identified. northwestern assays with a series of maltose-binding protein fusions that contain different ci fragments showed t ...	1996	8690088
the use of respiratory-tract cultures in the diagnosis of invasive pulmonary aspergillosis.	to define the role of lower-respiratory-tract cultures in the diagnosis of invasive pulmonary aspergillosis (ipa) in immunocompromised hosts.	1996	8629651
print-capture pcr: a simple and highly sensitive method for the detection of plum pox virus (ppv) in plant tissues.		1996	8668554
nonaldehyde sterilization of biologic tissue for use in implantable medical devices.	biologic tissue stabilized by dye-mediated photooxidation has found application in implantable devices. the desire to avoid aldehydes in the processing of photooxidized tissues led to the development of a nonaldehyde, iodine based sterilant. the interaction of tissue with iodine was indicated by a change in tissue shrinkage temperature, dependent upon solution and incubation parameters. the amino acid tyrosine also was altered, presumably because of aromatic ring iodination. transmission electro ...	1997	9116349
identification of three distinct antigenic sites in parapoxviruses.	monoclonal antibodies (mabs) were generated in balb/c mice immunized with gradient-purified particles, envelopes and cores of intracellular mature orf virus d-1701. three distinct antigenic sites were identified in this virus strain. their topographical relationships was determined by pairwise epitope specificity studies in competition elisas. one mab (class igm) neutralized virus infectivity. four micrograms/ml purified igm gave a 50% reduction of 100 pfu of orf virus d-1701. as shown by immuno ...	1997	9170506
a congenital persistent infection of bovine virus diarrhoea virus in pigs: clinical, virological and immunological observations.	we report on a lifelong 'carrier' state of non-cytopathic bovine virus diarrhoea virus (bvdv) in an otherwise healthy pig. three out of 13 pigs of a litter congenitally infected with bvdv survived for more than 3 months. one pig was bvdv seropositive at 1 month, the second seroconverted between 6 and 8 months, and the third remained viraemic and bvdv-immunotolerant until slaughter at 26 months. the latter pig, a boar, excreted virus in oropharyngeal fluid, urine and semen. ejaculates, however, d ...	1997	9323848
production of strain specific antibodies against a synthetic polypeptide corresponding to the n-terminal region of the plum pox potyvirus coat protein.	comparison of the predicted coat protein amino acid sequence of the 'sweet cherry' strain of plum pox potyvirus (ppv-swc) with the corresponding regions of several other ppv strains indicated that the main differences are in the n-terminal region. polyclonal antibodies were produced against a synthetic peptide corresponding to the 1-14 sequence of the n-terminal region of ppv-swc coat protein. they specifically detected ppv-swc in different immunochemical tests.	1997	9504763
susceptibility of peach gf 305 seedlings and selected herbaceous plants to plum pox virus isolates from western slovakia.	the susceptibility of peach gf 305 seedlings and herbaceous plants to five plum pox virus (ppv) isolates from orchards of western slovakia was investigated. ppv was isolated from diseased plum, apricot and peach trees, and transmitted by chip-budding to peach gf 305. the herbaceous plants were infected by mechanical inoculation. the transmission was analysed by symptomatology and double sandwich enzyme-linked immunosorbent assay (das-elisa). infected peaches developed leaf distortion, tissue cle ...	1997	9607094
the motif v of plum pox potyvirus ci rna helicase is involved in ntp hydrolysis and is essential for virus rna replication.	the plum pox potyvirus (ppv) protein ci is an rna helicase whose function in the viral life cycle is still unknown. the ci protein contains seven conserved sequence motifs typical of rna helicases of the superfamily sf2. we have introduced several individual point mutations into the region coding for motif v of the ppv ci protein and expressed these proteins in escherichia coli as maltose binding protein fusions. mutations that abolished rna helicase activity also disturbed ntp hydrolysis. no mu ...	1997	9358154
validation of the heat treatment step used in the production of diaspirin crosslinked hemoglobin (dclhb) for viral inactivation--effect of crosslinking.	two experiments were performed to assess viral inactivation during the crosslinking and heat treatment steps of the dclhb manufacturing process. stroma free hemoglobin (sfhb) collected from a large scale manufacturing lot was tested in a 1:680 scaled down system in which the key parameters used in the manufacturing process were replicated. in the first study porcine parvovirus (ppv), a non-enveloped virus, was used to assess inactivation, while in the second study bovine viral diarrhea virus (bv ...	1997	9352057
non-suppurative myocarditis in piglets associated with porcine parvovirus infection.	the involvement of porcine parvovirus (ppv) in the aetiology of non-suppurative myocarditis in sucking piglets was investigated by a polymerase chain reaction (pcr), designed to assess the presence of viral genome in formalin-fixed paraffin wax-embedded tissue of diseased animals. myocardium and lung of stillborn piglets with a confirmed ppv infection were used to set up the pcr amplification method. subsequently, 20 myocardia with inflammatory lesions were examined in parallel with 20 myocardia ...	1997	9352435
detection of antibodies against porcine parvovirus nonstructural protein ns1 may distinguish between vaccinated and infected pigs.	the humoral antibody response against the nonstructural protein ns1 and the structural protein vp2 of porcine parvovirus (ppv) was evaluated by immuno-peroxidase test (ipt) and enzyme linked immuno sorbent assay (elisa) using recombinant ppv antigens. the coding sequence for ns1 and vp2 was inserted into the baculovirus. autographa californica nuclear polyhedrosis virus (acnpv) genome resulting in two recombinant baculoviruses acnpv-ns1 and acnpv-vp2, respectively. sf9 cells (spodoptora frugidip ...	1997	9050166
virucidal treatment of blood protein products with uvc radiation.	the virus safety of blood derivatives continues to be of concern, especially with respect to nonenveloped and/or heat-stable viruses. previously, we demonstrated that treatment of whole plasma, ahf concentrate or fibrinogen with short wavelength ultraviolet light (uvc) results in the inactivation of > or = 10(6) infectious doses (id) of encephalomyocarditis virus (emcv), hepatitis a virus (hav) and porcine parvovirus (ppv), each of which is nonenveloped. protein recovery was enhanced greatly by ...	1997	9077126
simultaneous detection and typing of plum pox potyvirus (ppv) isolates by heminested-pcr and pcr-elisa.	two techniques for simultaneous detection and typing of plum pox potyvirus (ppv) isolates belonging to the d or m serotypes, heminested pcr (h-pcr) and pcr-elisa, have been developed. ten ppv isolates typed using ppv-d and ppv-m specific monoclonal antibodies by elisa-dasi were used to validate these two methods. the results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. when serial dilutions of infected plant extracts were assayed, h-pcr and ...	1997	9389402
long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis.	the 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (ppv) are highly conserved in all the members of the potyvirus genus. to map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. while we were not able to infect nicotiana clevelandii plants with full-length ppv transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome ...	1997	9201225
specific detection of d- and m-isolates of plum pox virus by immunoenzymatic determination of pcr products.	molecular techniques based on the polymerase chain reaction (pcr) can provide rapid and sensitive diagnosis of plum pox virus (ppv), the causal agent of the devastating 'sharka' disease of stone fruit trees. the present study compared routine polymerase chain reaction (pcr) procedures against a new system, pcr-elisa (boehringer mannheim), which enables immunoenzymatic detection of pcr products. the results show that this hybridisation system ensures fast and more sensitive detection of ppv assoc ...	1997	9300377
recombinant parvovirus-like particles as an antigen carrier: a novel nonreplicative exogenous antigen to elicit protective antiviral cytotoxic t cells.	to develop a strategy that promotes efficient antiviral immunity, hybrid virus-like particles (vlp) were prepared by self-assembly of the modified porcine parvovirus vp2 capsid protein carrying a cd8(+) t cell epitope from the lymphocytic choriomeningitis virus nucleoprotein. immunization of mice with these hybrid pseudoparticles, without adjuvant, induced strong cytotoxic t lymphocyte (ctl) responses against both peptide-coated- or virus-infected-target cells. this cd8(+) class i-restricted cyt ...	1997	9207121
comparison of two different methods for inactivation of viruses in serum.	in order to compare protocols for inactivation of viruses potentially present in biological specimens, three different model viruses were treated in bovine serum by two different inactivation methods: samples were subjected either to chemical inactivation with ethylenimine (el) at concentrations of 5 and 10 mm at 37 degrees c for periods up to 72 h or to electron-beam irradiation in frozen and liquid form with doses varying between 11 and 46 kgy. the chemical inactivation resulted in nonlinear t ...	1997	9302195
plum pox potyvirus resistance associated to transgene silencing that can be stabilized after different number of plant generations.	nicotiana benthamiana plants were transformed with a fragment of the plum pox potyvirus (ppv) genome that encodes the nuclear inclusion a (nia) and b (nib) proteins and the n-terminus of the capsid protein (nia-nib-cp). lines transformed with this ppv genomic fragment harboring mutations in the gdd replicase-motif were also obtained. plants of niadeltav lines that carry a gdd to vdd mutation in the ppv transgene, were immune to ppv infection. the resistance was highly specific, since it was only ...	1998	9469941
new methods for inactivation of lipid-enveloped and non-enveloped viruses.	two new methods are described for inactivating lipid-enveloped and non-enveloped viruses in plasma-derived products such as coagulation factors and intravenous immunoglobulin (igiv). iodine/sephadex delivers iodine to igiv solutions in a slow, controlled way and allows for inactivation of > or = 4 logs of porcine parvovirus (ppv), a hardy non-enveloped virus, under conditions which do not measurably damage the structural or functional properties of the igiv, and with essentially no iodination of ...	1998	9873761
relative concentration of plum pox virus in leaves and flowers of some prunus species and cultivars.	the relative concentration of plum pox virus (ppv) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (elisa) and expressed as the lowest dilution with positive reaction. significant differences in relative ppv concentration were found in leaves among individual prunus species naturally or artificially infected with the virus. the highest relative ppv concentration was found in blackthorns (7.81 x 10(-4)), com ...	1998	10073235
mineral nutrition of plum trees as an important factor of protection against plum pox virus disease.	the experimental results obtained from a fruit garden at krajné pointed out a different content of macrobiogenic elements in the leaves of plum trees infected with plum pox virus (ppv) as compared to healthy ones. mineral nutrition of diseased trees was characteristic by lower relational values of topical levels of macrobiogenic elements especially those of n/p, n/k, (ca + mg)/k, and (n/p)/(n/k).	1998	10073227
restriction fragment length polymorphism differentiation of plum pox virus isolates.	reverse transcription-polymerase chain reaction (rt-pcr) technique and restriction fragment length polymorphism (rflp) analysis were used to analyse six isolates of plum pox virus (ppv). whole coat protein (cp) gene was amplified in four isolates using the unipoty-polyt primer pari. ppv-d was identified by rflp analysis using sfui and drai enzymes in two of the isolates. two isolates of ppv-m strain yielded rt-pcr products which could not be digested by the two enzymes. other isolates were subje ...	1998	10073236
characterization of phenotype resistance to plum pox of transgenic plums expressing plum pox virus capsid gene.	resistance to plum pox virus (ppv) infection can be obtained in transgenic plants that express the virus capsid gene. an agrobacterium-mediated transformation was used to introduce the ppv capsid gene into prunus domestica plants. over 11 regenerated plants (clones) were observed for the development of the disease symptoms and analysed for the presence of ppv by enzyme-linked immunosorbent assay (elisa), western blot analysis, and reverse transcription-polymerase chain reaction (rt-pcr) through ...	1998	10073237
reproductive tract infections in primary healthcare, family planning, and dermatovenereology clinics: evaluation of syndromic management in morocco.	to determine where and with what symptoms women seek care for reproductive tract infections (rti) in morocco and to guide allocation of resources for training and treatment for rtis.	1998	10023358
some results of 50 years of research on the resistance to plum pox virus.	over 300 references on the resistance of stone fruit species to plum pox virus (ppv) have been utilized for the summarization of relevant information in this review. methods of testing ppv resistance, procedures of evaluation and characterization of ppv resistance as well as breeding of ppv-resistant cultivars are briefly discussed. altogether 370 cultivars, hybrids and clones of plum, peach, apricot, nectarine and wild prunus species are tabulated together with the authors who have reported on ...	1998	10073219
relationship between concentration of plum pox virus and content of pigments in virus-infected symptomatic apricot leaves.	besides other factors, occurrence of plum pox virus (ppv)-caused spots and mosaic symptoms on leaves of stone fruits are known to influence important physiological functions including production of assimilates. apricot (prunus armeniaca l.) seedlings were used as a test material for typical manifestation of symptoms of the disease on the foliage. the relations of the abovementioned visual symptoms to the virus and pigments concentrations in leaves have so far not been known sufficiently. we dete ...	1998	10073220
comparison of different methods of rna isolation for plum pox virus detection by reverse transcription-polymerase chain reaction.	the diagnosis of plum pox virus (ppv) is still considered one of the most important aspects of the "sharka" problem. in fact, different studies demonstrated an uneven distribution of the virus in infected trees due to a high variability in virus concentration. these aspects complicate the ppv diagnosis. to date, biological, serological and molecular assays have been successively developed in order to obtain sensitive and efficient ppv detection techniques. in particular, the polymerase chain rea ...	1998	10073221
investigation on the plum pox virus resistance in different apricot genotypes.	in our three-year investigation, 164 apricot trees of different old german varieties cultivated in the mansfelder land region were tested for the plum pox virus (ppv) resistance by double grafting in greenhouse conditions using an isolate of ppv d strain from our region. we selected 25 genotypes with quantitative resistance and two with immunity. the first results of field trials are comparable with those from greenhouse. with cvs. virosia and brevira, two local quantitatively resistant varietie ...	1998	10073222
preliminary report on the apparent breaking of resistance of a transgenic plum by chip bud inoculation of plum pox virus ppv-s.	five transgenic clones of prunus domestica l. containing plum pox virus (ppv) coat protein (cp) gene and one non-transformed control clone were challenged with ppv-s in the field. symptoms developed on c2, c3, c4, c6 and b70146 but not c5 trees inoculated by chip budding (cbi) (2/2, 2/2, 1/1, 2/2 and 2/2, positive/inoculated) in the first summer after inoculation. however, in the second year, symptoms appeared on cbi c5 trees. the presence of the virus in the plants was confirmed by enzyme-linke ...	1998	10073228
new results concerning the plum pox virus epidemiology and resistance of plum cultivars, hybrids and rootstocks.	our recent results on the plum pox virus (ppv) epidemiology show that ppv spreads very rapidly in plum tree plantations in the contaminated areas. a clearing of the ppv-infected trees reduces significantly the spread of the virus but does not eliminate the disease. some plum tree cultivars, hybrids and rootstocks (scoldus, alina, cristi, bn 1/8fl, bn 2gr. etc) showing field resistance could not be infected with ppv by natural way. however, they could be infected with ppv by artificial inoculatio ...	1998	10073229
characterization of plum pox virus isolates from slovakia.	plum pox virus (ppv) isolates from stone-fruit trees (plums, myrobalans, apricots and peaches) from orchards and gardens were characterized. to characterize their biological properties, several ppv isolates were transmitted by chip budding to gf 305 seedlings and mechanically to selected herbaceous test plants. the isolates differed in severity of infection, host range and symptomatology. a subgroup differentiation of 43 isolates from 22 localities of western and middle slovakia was accomplished ...	1998	10073223
aphid species--vectors of plum pox virus.	plum pox virus (ppv) is widely spread by natural vectors present in plum orchards. the efficiency of transmission is dependent on the frequency of the occurrence of vectors and the cultivar susceptibility to this pathogen. having in view that ppv has a wide range of annual and multiannual host plants and vectors, there is great concern for obtaining ppv-resistant cultivars. this report deals with the following vectors: hyalopterus pruni, brachycaudus cardui, brachycaudus helichrysi. myzus persic ...	1998	10073225
removal of viruses from human intravenous immune globulin by 35 nm nanofiltration.	viral safety is an important prerequisite for clinical immunoglobulin preparations. a common manufacturing practice is to utilize several virus removal/inactivation process steps to ensure the safety of human intravenous immunoglobulin (ivig). in this regard, we examined the use of planova 35 nm filters to reduce potential loads of both non-enveloped and enveloped viruses prior to end-stage solvent detergent treatment. the nanofiltration process was validated for removal of a variety of envelope ...	1998	10403036