Publications

primary structure of an ecori fragment of lambda imm434 dna containing regions ci-cro of phage 434 and cii-o of phage lambda.	digestion of phage lambda imm434 dna with restriction endonuclease ecori yields 7 fragments. the shortest among them (1287 bp) contains the right part of the phage 434 immunity region and the phage dna portion proximal to it. the complete primary structure of this fragment has been determined using the chemical method of dna sequencing. hypothetical amino-acid sequences of proteins coded by the cro gene of phage 434 and the cii gene of phage lambda, as well as nh2-terminal amino-acid sequences o ...	1979	478301
operators and promoters in the or region of phage 434.	the or operator region of phage 434 contains three 14 bp blocks with sequence acaaga-a--ttgt which are presumed to be the 434 repressor recognition sites. operator constitutive mutations are located in two of these blocks, while a mutation affecting repressor levels in the lysogenic state is located in the third. two transcripts obtained in vitro, one leftwards and one rightwards, are tentatively identified as the prm and pr transcription starts. the arrangement of the 434 operator region appear ...	1979	450705
the site controlling the specificity of n action is outside the promoter-operator region: a triple hybrid phage lambda n21 imm434nin5.	a short interval of homology between imm lambda, imm434 and imm21 dnas was identified near the leftward promoter-operator region. this homology, denoted hs, was revealed by electron microscopic examination of lambda imm lambda/lambda imm21 and lambda imm434/lambda imm21 heteroduplexes, and permitted us to construct a special lambda hybrid (lambda hyb) which contains the n region of phage 21 and the adjacent imm region from phage 434. this triple hybrid, labmda n21 imm434nin5, was analysed by gen ...	1979	381108
nucleotide sequence of the cro-cii-oop region of bacteriophage 434 dna.	the nucleotide sequence of a 869 bp segment of phage 434 dna including the regulatory genes cro and cii is presented and compared with the corresponding part of the phage lambda dna sequence. the 434 cro protein as deduced from the dna sequence is a highly basic protein of 71 amino acid residues with a calculated molecular weight of 8089. while the cro gene sequences of phage 434 and lambda dna are very different, the nuleotide sequences to the right of the lambda imm434 boundary show difference ...	1979	375198
major outer membrane proteins of e. coli k12 serve as receptors for the phages t2 (protein ia) and 434 (protein ib).	mutants of e. coli resistant to bacteriophage t2 have lowered amounts of protein ia in their outer membrane. bacteriophage t2 was inactivated by a mixture of protein ia-lipopolysaccharide. protein ia or lipopolysaccharide alone had no neutralizing activity. however, only protein ia was required to inactivate a t2 host range mutant. in the presence of polymyxin b t2 receptor activity of protein ia--lipopolysaccharide mixtures could not be restored. e. coli strains missing protein ib were resistan ...	1978	360042
purification and some properties of presumptive tof gene product of coli phage 434.	the presumptive tof gene product of coli phage 434 has been purified from cells carrying lambdaimm434cidv plasmid known to contain only some of the "early" genes of phage 434 and lambda. it was detected and tentatively identified as tof protein primarily by its ability to specifically bind to phage 434 dna. the protein has a molecular weight of about 11,000 and requires mg2+ for specific dna binding, unlike 434 ci-repressor.	1977	340902
control of ci gene expression in bacteriophage lambda imm434, studied in an immunity/trp fusion made in vitro.	the trp genes of a lambda imm434 trp-transducing phage have been fused to the immunity region by deletion, in vitro, of the dna between two targets for the restriction enzyme r.ecori. the resulting phage has been used to study the control of expression of the ci gene in vivo. the constitutive rate of expression of the ci gene is between 2 and 5% of the maximally stimulated rate. the products of the cii and ciii genes enhance expression of ci on infection of a sensitive host. the requirement for ...	1976	785219
cro mutants of phage 434.		1975	1189298
in vitro assembly of bacteriophage lambda heads.	the assembly of plaque-forming particles in cell-free extracts of induced lambda lysogens was observed two ways. (i) dna isolated from a lambda-related phage, 434 for example, is added to an extract of an induced lambda lysogen, and plaque-formers with the genotype of the added dna are detected. (ii) one extract from an induced lambda lysogen that carries an amber mutation in one of the head genes (a, b, c, d, or e) is mixed with one carrying an amber mutation in a different head gene; an increa ...	1973	4509659
mapping of deletions and substitutions in heteroduplex dna molecules of bacteriophage lambda by electron microscopy.	electron microscopy of heteroduplex dna molecules, composed of one strand of escherichia coli phage lambda(+) dna annealed to the complementary dna strand of a lambda deletion or substitution mutant, permits visualization, as well as precise measurements and mapping, of the unpaired single-stranded regions of nonhomology in the otherwise double-stranded molecules. in the lambdab2 mutant, the central segment (13 percent) of the lambda(+) dna molecule is shown to be deleted. in the hybrid phages l ...	1969	5765116
purification and properties of a deoxyribonucleic acid exonuclease associated with the formation of phage 434.		1964	14257619