Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human papillomavirus: detection of viral dna sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix. | colposcopic-directed biopsies of the uterine cervix from 22 patients were analyzed for the presence of human papillomavirus (hpv) dna and structural antigens. 11 of the biopsies were classified microscopically as mild dysplasia, 3 as moderate dysplasia, 1 as severe dysplasia, and 7 as squamous metaplasia. nonstringent hybridization with a bovine papillomavirus type 1 dna probe and immunocytochemical analysis with an antiserum against papillomavirus genus-specific structural antigens were perform ... | 1983 | 6317605 |
| a papillomavirus dna from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. | dna from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (hpv) type 11 dna only under nonstringent conditions. this dna was molecularly cloned in lambda phage. under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%) with hpv types 10, 14, and 15 and showed no homology with dna of other human hpv types. we therefore propose to designate it tentatively as hpv 16. hpv 16 dna was used as a probe t ... | 1983 | 6304740 |
| detection of human papillomavirus dna sequences in conjunctival papilloma. | two histologically proven conjunctival papillomas (one from a 33-month-old boy and the other from a 28-year-old woman) were examined for the presence of papillomavirus by dna molecular hybridization. the first case, a recurrent tumor known to be positive for papillomavirus structural antigen, demonstrated human papillomavirus dna sequences that cross-hybridized to a human papillomavirus type 11 dna probe. the second case, an initial tumor, which was negative for papillomavirus structural antigen ... | 1983 | 6314814 |
| specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: a preliminary communication. | cervical papanicolaou smears and paraffin sections of biopsy specimens obtained from women attending dysplasia clinics were examined for viral dna sequences by in situ hybridization technique using 35s-labeled cloned recombinant dna probes of human papillomavirus (hpv) types 6, 11, and 16. these and one unrelated dna probe complementary to measles virus rna were labeled by nick translation using either one or two 35s-labeled nucleotides. the radiolabeled probes were reduced in size with dnase to ... | 1985 | 2997054 |
| papillomavirus infection of the anogenital region: correlation between histology, clinical picture, and virus type. proposal of a new nomenclature. | the clinical and histologic picture of 84 anogenital condylomatous and condyloma-like lesions of both sexes were analyzed in an effort to establish a correlation to the different papillomavirus (pv) types. the presence of human papillomavirus (hpv)-specific dna sequences was confirmed through molecular hybridization and the presence of pv structure antigens was verified in thin sections by means of a group-specific anti-pv-antiserum using the peroxidase-antiperoxidase (pap) technique. three dist ... | 1985 | 2991390 |
| [virus papillomas and malignant genital tumors]. | morphological and epidemiological studies point to a close correlation between viral genital warts and genital cancer. benign condylomata acuminata are commonly induced by hpv-6 and hpv-11. in precancerous bowenoid genital lesions, hpv-16 and hpv-18 could be cloned directly from cervical cancer tissue. whereas the occurrence of hpv-6 and hpv-11 seems to be a low risk infection, hpv-16 and hpv-18 may represent a high risk for malignant conversion. | 1985 | 3006372 |
| persistence and expression of human papillomavirus dna in genital cancer. | there is mounting evidence that certain types of human papillomaviruses (hpv types 16 and 18) are associated with human genital cancer. other virus types, such as hpv-6 or hpv-11, are more regularly found in benign genital warts. since all viruses can be present in putative precancerous lesions of the uterine cervix (dysplasia, cervical intraepithelial neoplasia) it has been postulated that individual hpv types have different 'oncogenic potential'. the molecular basis for this difference is not ... | 1986 | 3013522 |
| [urogenital and anal papillomavirus infections]. | recently virologists and clinicians have focused attention on infections with human papillomaviruses (hpv). this is due to the ubiquity, the increasing frequency and the possible association of these viruses with the development of squamous cell carcinomas of the skin and of the mucous membranes of the respiratory, gastrointestinal, genitourinary and anorectal tracts. hpv represent a very heterogeneous group of dna tumor viruses. by means of molecular-biological techniques, more than 40 hpv type ... | 1986 | 3026998 |
| human papillomaviruses in buschke-löwenstein tumors: physical state of the dna and identification of a tandem duplication in the noncoding region of a human papillomavirus 6 subtype. | six buschke-löwenstein tumors, i.e., highly differentiated squamous cell tumors of the genital region, were shown to contain human papillomavirus 6 (hpv 6) or hpv 11 genomes. the viral dna was found in an episomal state, including a very small fraction of circular oligomers. hpv 6a and hpv 6d genomes were cloned from two of the tumors. comparison with hpv 6b, cloned from a benign genital wart (e. -m. de villiers, l. gissmann, and h. zur hausen, j. virol. 40:932-935, 1981) by restriction mapping ... | 1986 | 3009899 |
| [papilloma viruses in benign and malignant tumors of the mouth and upper respiratory tract]. | a total of 113 benign and malignant tumours of the mouth and upper respiratory tract have been analysed for the presence of papilloma virus dna. thirty-four (63%) of 54 papillomatous lesions were found to contain such dna. seventy-two percent of the laryngeal papillomas contained hpv 6 or hpv 11 dna, whereas the oral papillomatoses harboured hpv 6 (15.6%), hpv 11 (9.4%), hpv 7 (9.4%), hpv 13 (12.5%) and hpv 32 (9.4%) dnas. the high number of hpv 7 dna-positive lesions was unexpected. in most of ... | 1986 | 3012230 |
| in vivo transformation of human skin with human papillomavirus type 11 from condylomata acuminata. | human papillomaviruses (hpvs) have been implicated in the development of a number of human malignancies, but direct tests of their involvement have not been possible. we describe a system in which human skin from various sites was infected with hpv type 11 (hpv-11) extracted from vulvar condylomata and was grafted beneath the renal capsule of athymic mice. most of the skin grafts so treated underwent morphological transformation, resulting in the development of condylomata identical to those whi ... | 1986 | 3016303 |
| laboratory production in vivo of infectious human papillomavirus type 11. | human papillomaviruses (hpv) induce among patients natural lesions which produce small amounts of virus. infection of human cell cultures does not lead to the multiplication of virus, which also does not replicate in experimental animals. we have developed a unique system for the laboratory production of hpv type 11 (hpv-11). fragments of human neonatal foreskin were infected with an extract of naturally occurring human vulvar condylomata and grafted beneath the renal capsule of athymic mice. la ... | 1987 | 3027386 |
| susceptibility of various human tissues to transformation in vivo with human papillomavirus type 11. | human papillomaviruses (hpvs) are associated with proliferative lesions in a variety of human epithelial types. in some cases, the associations are highly specific in that a certain papillomavirus type may infect only one human epithelial cell phenotype. in other cases, one papillomavirus apparently infects several tissue types and not others. further, hpv replication capacity varies considerably in different epithelia. due to the lack of a suitable method, it has not been possible, until now, t ... | 1987 | 3030939 |
| characterization of human papilloma virus types in condylomata acuminata in children by in situ hybridization. | the presence of human papilloma virus (hpv) dna sequences of types 6, 11, 16, and 18 was determined by in situ hybridization under stringent conditions on archival paraffin-embedded tissue sections in eight condylomata acuminata observed in children below the age of 12 years. viral sequences were detected in seven of eight cases: all of them contained hpv 6, four contained also hpv 11, and one contained hpv 16 and 18. papilloma virus common antigen was detected in only three of eight cases, all ... | 1987 | 3037191 |
| a human papilloma virus type 11 transcript encoding an e1--e4 protein. | the human papilloma virus (hpv) associated with a genital wart (condyloma acuminatum) was determined to be type 11. the majority of the viral dna molecules were monomeric circles present in the cells at high copy number, as demonstrated by one- and two-dimensional agarose gell electrophoretic separation followed by southern blot analysis. a cdna library in phage lambda gt11 was constructed from poly(a)-selected mrna recovered from the tissue. recombinant clones corresponding to the most abundant ... | 1987 | 2887066 |
| human papillomavirus-11 dna in a patient with chronic laryngotracheobronchial papillomatosis and metastatic squamous-cell carcinoma of the lung. | 1987 | 2442608 | |
| the clinical management and laboratory assessment of anal warts. | the results of a clinical and virological survey of anal warts from 85 predominantly homosexual or bisexual men is presented, together with an improved technique for the surgical treatment of these lesions. two types of anal warts, which were classified as either "acute" or "chronic" on the basis of their appearance and clinical behaviour, were recognized commonly. however, our laboratory investigations--which consisted of the routine histopathological examination of all specimens, together with ... | 1987 | 3037287 |
| venereal papilloma and papillomavirus in a colobus monkey (colobus guereza). | a papilloma on the penis of a colobus monkey was found to contain papilloma-virus group specific antigens by immunohistochemical analysis and virus-like particles in the nuclei of epithelial cells by transmission electron microscopy. in low-stringency southern blot hybridizations, dna from the lesion annealed with human papillomavirus 11 dna, but not with the dnas of 13 other papillomaviruses. using human papillomavirus 11 dna as a probe in southern blot hybridizations, dna from the penile papil ... | 1987 | 2835331 |
| human papillomavirus (hpv) infections and their associations with squamous cell neoplasia. | current data implicating an etiological role of human papillomavirus (hpv) infections in precancer lesions (intraepithelial neoplasia) and squamous cell carcinoma of both the genital tract and other sites (oral cavity, larynx, skin, esophagus, nasal cavity, bronchus) can be summarized as follows: a) hpv involvement in benign, precancer, and malignant genital squamous cell lesions has been demonstrated by morphological, immunohistochemical and dna hybridization techniques; b) hpv infections in th ... | 1987 | 2829787 |
| verrucous carcinoma of the vulva containing human papillomavirus-11. case report. | 1988 | 2838068 | |
| identification of human papillomavirus type 11 e4 gene products in human tissue implants from athymic mice. | a new method has recently been described for the growth of human papillomavirus type 11 (hpv11), an agent associated with genital warts, in human tissue xenografts implanted under the renal capsules of athymic nude mice (j. w. kreider et al., 1986, j. virol. 59, 369-376; 1987, j. virol. 61, 590-593). with this model it is now possible to study productive hpv11 infection under controlled laboratory conditions. to identify proteins encoded by the hpv11 e4 open reading frame in infected implants, w ... | 1988 | 2838963 |
| regulation of human papillomavirus type 11 enhancer and e6 promoter by activating and repressing proteins from the e2 open reading frame: functional and biochemical studies. | e2-c, a protein consisting mainly of the carboxy-terminal 45% of the human papillomavirus type 11 (hpv-11) e2 protein, was expressed from the rous sarcoma virus long terminal repeat in mammalian cells. it competitively repressed the stimulatory action of the full-length e2 protein on the hpv-11 enhancer located in the upstream regulatory region, as assayed by the expression of a reporter gene from the simian virus 40 (sv40) early promoter in transiently transfected monkey cv-1 cells. a mutation ... | 1988 | 2839716 |
| the responsiveness of human papillomavirus upstream regulatory regions to herpes simplex virus immediate early proteins. | we have tested the responsiveness of the upstream regulatory regions (urr) of human papillomaviruses (hpv) to transactivation by the herpes simplex virus type 1 (hsv-1) immediate early proteins icp4 (vmw175) and icp0 (vmw110), using recombinant plasmids which contain urrs of hpv 1, 11 or 16 upstream of an sv40-promoted cat gene. transfection of the hpv-cat plasmids into mouse cells expressing a temperature-sensitive icp4 protein enabled us to demonstrate that icp4 enhances transcription of the h ... | 1988 | 2848381 |
| mapping of two novel transcripts of bovine papillomavirus type 4. | a cdna library was synthesized using rna from bovine papillomavirus type 4 (bpv-4)-induced papillomas. the major viral transcript was characterized by sequencing of its cdna, primer extension mapping and s1 nuclease protection studies. the transcript initiates at multiple sites between nucleotide (nt) 777 and nt 902, contains a splice junction between nt 1016 and nt 3376 and terminates at nt 4034, using the early polyadenylation signal at nt 4009. sequencing of viral cdnas and mrna revealed devi ... | 1988 | 2848926 |
| sexual transmission of human papillomaviruses in heterosexual and male homosexual couples, studied by dna hybridisation. | the prevalence of human papillomavirus (hpv) 6, 11, 16, and 18 in 36 heterosexual couples and seven male homosexual couples with genital warts was investigated for evidence of sexual transmission of genital hpv. the prevalence of virus type and number of copies of viral genome equivalents/cell in the lesions were assessed, and the factors influencing transmission analysed. our results show that hpv 6 and, to a lesser extent, hpv 11 were the types most readily transmitted, and that transmission a ... | 1988 | 2831137 |
| functional mapping of the human papillomavirus type 11 transcriptional enhancer and its interaction with the trans-acting e2 proteins. | the transcriptional enhancer sequences of the papillomaviruses are regulated by trans-acting factors encoded by the viral e2 open reading frame. we have performed detailed functional and physical analyses of the enhancer of the human papillomavirus type 11 (hpv-11). using the chloramphenicol acetyltransferase (cat) assay in transiently transfected monkey cv-1 cells, the enhancer region has been localized to a 270-bp tract immediately preceding the e6 open reading frame, and it consists of two fu ... | 1988 | 2833426 |
| glucocorticoid-dependent oncogenic transformation by type 16 but not type 11 human papilloma virus dna. | squamous cell carcinoma of the uterine cervix is one of the most common cancers among women. correlation between human papilloma virus (hpv) infection of the uterine cervix and the development of cervical neoplasia has been established. more recent studies have shown the presence and expression of integrated hpv types 16 and 18 dna sequences in 70-80% of cervical tumours and tumour cell lines. it has been suggested that, in addition to hpvs, other agents such as hormones and tobacco products act ... | 1988 | 2847052 |
| isolation and characterization of a variant of human papillomavirus type 11 from a nasal inverting (schneiderian) papilloma. | we have previously reported the presence of a variant of human papillomavirus (hpv) type 11 in a nasal inverting papilloma [respler et al., 1987]. in the present study, we have cloned molecularly the dna of this variant at its unique restriction enzyme bam hi site into lambda bf101 phage. restriction enzyme mapping and dna sequencing revealed that the genome of this virus contained an extra 531 base pair (bp) which was the repeat of most of the noncoding region (ncr) of hpv 11. insertion of tran ... | 1988 | 2839608 |
| sexually transmitted papillomaviral infection in the male: ii. the urethral reservoir. | urethral cytobrushing for hybridization studies was obtained from the anterior urethra of 75 male consorts of known hpv-infected female partners. our findings revealed a 24 per cent (19 patients) incidence of positive hybridization, identifying hpv-16 or hpv-18 in six instances associated with mixed papillomaviral infection of the shaft and the presence of hpv-6 and hpv-11 in 7 cases clinically related to exophytic disease of the shaft. although no histologic evidence of virus-tissue interaction ... | 1988 | 2838945 |
| laryngeal papillomatosis: correlation between severity of disease and presence of hpv 6 and 11 detected by in situ dna hybridisation. | a technique using a biotin-streptavidin polyalkaline phosphatase complex was applied to routinely fixed and processed biopsy specimens of laryngeal papillomata from 45 patients taken over the past 20 years to detect human papilloma virus (hpv) types 6 and 11. two thirds of both adult and juvenile onset cases were positive for hpv 6 or hpv 11 or both. five specimens of normal vocal cord epithelium were negative for hpv 6 and 11. the detailed clinical history, endoscopic findings, success of treat ... | 1989 | 2547844 |
| characterization of an hpv type 11 isolate propagated in human foreskin implants in nude mice. | human papillomavirus type 11-hershey (hpv-11-h) from an extract of genital warts has been serially passaged by infecting human foreskin chips that are then implanted in athymic mice (j. w. kreider, m. k. howett, a. e. leure-dupree, r. j. zaino, and j. a. weber (1987), j. virol. 61, 590). subsequent attempts to propagate hpvs present in other human lesions have not been successful. in an effort to identify the basis for the seemingly unique ability of hpv-11-h to propagate in the xenografts, we c ... | 1989 | 2545034 |
| tissue site-specific enhancer function of the upstream regulatory region of human papillomavirus type 11 in cultured keratinocytes. | the human papillomavirus type 11 regulatory region was cloned upstream of a reporter complex and microinjected into nuclei of individual primary human keratinocytes. genital and laryngeal keratinocytes, normal host cells for this virus, exhibited higher levels of expression than cutaneous keratinocytes. we conclude that a papillomavirus enhancer(s) shows preferences among epithelial cells from different tissue sites. | 1989 | 2536117 |
| in situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas. | routinely processed paraffin sections from 20 patients with adult laryngeal papillomas were examined for the presence of human papillomavirus type 11 (hpv-11) dna and its specific mrna by in situ hybridization methods using 35s-labeled rna probes. immunohistochemical techniques were also used to identify papillomavirus genus-specific common antigen (pgs-antigen). hpv-11 dna signals and/or papillomavirus genus-specific common antigen were detected in all eight samples of multiple laryngeal papill ... | 1989 | 2535884 |
| the polymerase chain reaction: a new epidemiological tool for investigating cervical human papillomavirus infection. | the polymerase chain reaction is an in vitro method for primer directed enzymatic amplification of specific target dna sequences. the technique was used to detect human papillomavirus types 11 and 16 simultaneously in cellular dna recovered from cervical smears in 38 women referred for colposcopy to evaluate cytological abnormality and 10 women with no history of cytological abnormality. the polymerase chain reaction was shown to be both specific and sensitive in detecting human papillomavirus d ... | 1989 | 2537123 |
| human papillomavirus type 11 dna in squamous cell carcinomas and pre-existing multiple laryngeal papillomas. | the case histories of 4 patients are presented, who developed an invasive squamous cell carcinoma from pre-existing multiple laryngeal papillomatosis (two juvenile-onset and two adult-onset) during a long latency period. a series of 14 routinely processed, paraffin-embedded biopsies obtained from these patients (including both benign papillomas and carcinomas) were analysed using in situ dna-hybridization technique with 35s-labelled human papillomavirus (hpv) dna probes of hpv types, 6, 11, 16, ... | 1989 | 2538994 |
| chronic cavitary respiratory papillomatosis. | a 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. he was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. a lobectomy was performed. the smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ dna hybridization. f ... | 1989 | 2552955 |
| occult human papillomavirus infection of the uterine cervix in postmenopausal women. | occult infection is assumed when human papillomavirus is detected in the absence of unequivocal cytologic or histologic changes. in this study occult human papillomavirus infection in postmenopausal women was examined to determine if the histologic findings with such infections were indeed nondiagnostic and to compare the rate of occult human papillomavirus infection in this group with that in a younger age group. in five of 43 cases (12%) sequences homologous to human papillomavirus deoxyribonu ... | 1989 | 2537012 |
| characterization of integrated human papillomavirus type 11 dna in primary and metastatic tumors from a renal transplant recipient. | a primary perianal squamous cell carcinoma and two metastatic tumors from a renal transplant recipient with a previous history of condyloma acuminatum were analyzed by filter hybridization for the presence of human papillomavirus (hpv) dna. each of the dna extracts from these three tissues was found to contain hpv dna. stringent hybridization and restriction endonuclease analysis identified this viral dna as hpv 11 related, which largely comigrated with cellular dna, suggesting the presence of i ... | 1989 | 2539906 |
| identification of a novel constitutive enhancer element and an associated binding protein: implications for human papillomavirus type 11 enhancer regulation. | the human papillomavirus type 11 enhancer, when linked to the minimal simian virus 40 early promoter, has been dissected into two domains in monkey kidney cv-1 cells, one being constitutive (designated cei) and the other inducible by trans-acting e2 proteins encoded by homologous and heterologous papillomaviruses (h. hirochika, t.r. broker, and l.t. chow, j. virol. 61:2599-2606, 1987; h. hirochika, r. hirochika, t.r. broker, and l.t. chow, genes dev. 2:54-67, 1988). we have demonstrated that the ... | 1989 | 2542607 |
| characterization of rare human papillomavirus type 11 mrnas coding for regulatory and structural proteins, using the polymerase chain reaction. | certain human papillomavirus (hpv) types cause warts, dysplasias, and carcinomas of the ano-genital and oral mucosa. because of the inability to propagate hpvs in cultured cells, the paucity of viral mrnas in human lesions, and the complexity of alternatively spliced transcripts derived from different promoters, it has not been possible to ascertain the exact structures of the majority of the mrna species and the proteins encoded. we have adapted the recently developed polymerase chain reaction ... | 1989 | 2552659 |
| two different human papillomavirus (hpv) types associated with oral mucosal lesions in an hiv-seropositive man. | different types of human papillomaviruses (hpv) are associated with a variety of oral lesions. so far, hpv types 1, 2, 4, 6, 7, 11, 13, 16, 18, 32 and 57 have been identified in oral lesions. immunosuppression predisposes oral mucosa to clinical manifestation of different virus infections including hpv. we describe here a 30-year-old hiv-positive and immunosuppressed man, who had suffered from oral lesions for a few months. on clinical examination, a nodular elevation was detected on the lower l ... | 1989 | 2553934 |
| dna dot-blot hybridization implicates human papillomavirus type 11-dna in epithelioma cuniculatum. | epithelioma cuniculatum is a rare, low-grade squamous cell carcinoma, belonging to the group of verrucous carcinomas. evidence is presented that suggests that human papilloma virus type-11 may be involved in the pathogenesis of epithelioma cuniculatum. hpv-dna was detected by dot-blot hybridization with 32p-labelled probe dna. plasmids containing hpv-dna were isolated by the benzoylated naphthoylated deae cellulose method (bnd-method), an improved procedure for routine detection of hpv-dna. | 1989 | 2555444 |
| detection of human papillomavirus type 11 dna in a conjunctival squamous cell papilloma by in situ hybridization with biotinylated probes. | a 44-year-old man presented with an exophytic papillomatous growth in the tarsal conjunctiva of his right eye. the tumour was excised and subjected to histological examination. on light microscopy, a squamous cell papilloma without signs of dysplasia was disclosed, because the entire tissue block was cut into sections, the performance of a conventional in situ hybridization for detection of human papillomavirus (hpv) dna was not possible. an alternative approach was used to demonstrate the prese ... | 1989 | 2801046 |
| anal condylomas in men. 1. histopathological and virological assessment. | a series of 128 biopsy specimens from anal condylomas in 73 homosexual or bisexual and 38 heterosexual men (mean (sd) age 31.8 (9.6) years) were subjected to histological assessment and human papillomavirus (hpv) typing by in situ dna hybridisation with 35s-labelled hpv 6, 11, 16, 18, 31, and 33 probes. most patients were also tested serologically for antibodies to human immunodeficiency virus (hiv). as evaluated on light microscopy, most (74%, 95/128) of the lesions were exophytic (papillary) a ... | 1989 | 2553581 |
| identification of dna-protein interactions and enhancer activity at the 5' end of the upstream regulatory region in human papillomavirus type 11. | we have examined the 5' end of the noncoding region of the genome of a human papillomavirus, hpv-11, for regulatory elements using permissive host cells. this region of unknown function in the upstream regulatory region (urr) is known to have unusual dna structure and frequently contains rearrangements which are associated with some more virulent isolates. this 5' 269-bp fragment was found to exhibit both specific dna-protein binding using laryngeal papilloma protein extracts and enhancer activi ... | 1989 | 2541536 |
| human papilloma virus type 11 in a fatal case of esophageal and bronchial papillomatosis. | we report a case of an apparently healthy 27-year-old man with a fatal course of papillomatosis, originating in the distal part of the esophagus and spreading into the main and intermediate bronchus. human papillomavirus type 11, usually associated with juvenile laryngeal papillomatosis and genital condyloma acuminatum, was detected in the papillomas. in spite of treatment with co2-laser evaporation of the papillomas, and with systemic as well as topical interferon, vp-16 and bleomycin, the papi ... | 1989 | 2543063 |
| characterization of cdnas of spliced hpv-11 e2 mrna and other hpv mrnas recovered via retrovirus-mediated gene transfer. | human papillomaviruses (hpvs) are associated with hyperproliferations of cutaneous or mucosal epithelium. these viruses cannot be propagated in any cell culture system. because cloning cdna copies of hpv mrnas recovered from human lesions has met with only very limited success, the characterization of hpv mrnas has been problematic. using the moloney murine leukemia virus vector system (c.l. cepko, b.e. roberts, and r.c. mulligan, 1984, cell 37, 1053-1062), we have recovered cdnas of spliced e2 ... | 1989 | 2552658 |
| infectious cycle of human papillomavirus type 11 in human foreskin xenografts in nude mice. | we have performed the first molecular analysis of a time course of infection by a papillomavirus. the hershey isolate of the human papillomavirus type 11 was used to infect human foreskin tissues, which were then implanted under the renal capsules of nude mice. the xenografts were recovered every 2 weeks for 14 weeks, fixed in formalin, and embedded in paraffin. four-micrometer serial sections were examined by light microscopy for morphological changes, by immunocytochemistry for virion antigen ... | 1990 | 2161943 |
| expression of the full-length products of the human papillomavirus type 6b (hpv-6b) and hpv-11 l2 open reading frames by recombinant baculovirus, and antigenic comparisons with hpv-11 whole virus particles. | the l2 open reading frames (orfs) of human papillomavirus (hpv) types 6b and 11 were expressed as full-length non-fusion proteins in spodoptera frugiperda (sf-9) cells using recombinant baculovirus. both proteins were detected on western blots as immunoreactive bands which migrated with apparent mrs of 76k and 78k, respectively, and contained both cross-reactive and type-specific epitopes, as determined by polyclonal antisera directed against defined subregions of the hpv-6b and hpv-11 l2 orfs. ... | 1990 | 2174962 |
| monoclonal antibody-mediated neutralization of infectious human papillomavirus type 11. | monoclonal antibodies recognizing human papillomavirus type 11 (hpv-11) were prepared from balb/c mice immunized with intact hpv-11 virions obtained from morphologically transformed human foreskin xenografts grown subrenally in athymic mice. four of five monoclonal antibodies that were reactive by enzyme-linked immunosorbent assay only to intact virions neutralized hpv-11 infectivity in the athymic mouse xenograft system. | 1990 | 2170694 |
| human papilloma virus in erosive oral lichen planus. | several types of human papilloma viruses (hpv) have been associated with benign and malignant squamous cell tumours of mucosal epithelium. to identify hpv in erosive oral lichen planus (olpe), considered as a premalignant lesion, tissues from 20 patients were examined by southern blot hybridization with 32p-labeled hpv dna probes. type 11 was found in 6 of the lesions while hpv types 6, 16 and 18 were not detected in any of the tissues examined. using a type-specific polymerase chain reaction (p ... | 1990 | 2169530 |
| treatment of latent rabbit and human papillomavirus infections with 9-(2-phosphonylmethoxy)ethylguanine (pmeg). | the acyclic nucleotide pmeg was studied for effectiveness against shope papillomavirus (crpv) infection of rabbits and human papillomavirus type 11 (hpv-11) infections of human foreskin xenografts in athymic mice. pmeg given in the latent period strongly suppressed the subsequent growth rates of shope papillomas. pmeg starting in the latent period and continuing for the duration of the experiment, inhibited hpv-11 infections of human skin, including condyloma growth, and synthesis of viral dna a ... | 1990 | 1964372 |
| immortalization of primary rat embryo cells by human papillomavirus type 11 dna is enhanced upon cotransfer of ras. | transfer of human papillomavirus type 11 (hpv11) dna and a neo(r) marker into primary rat embryo cells (rec) led to colony formation in g418-selective medium. about 20% of hpv11 clones were eventually established in culture but displayed low growth rates. cotransfection of hpv11 dna and an activated ras oncogene led to formation of both drug-resistant flat colonies and phenotypically transformed clones which grew efficiently when expanded in culture. a number of transformants reverted to a flat, ... | 1990 | 1695409 |
| antibody-mediated neutralization in vivo of infectious papillomaviruses. | specific antibody-mediated neutralization of infectious human papillomavirus type 11 (hpv-11) was achieved in the athymic mouse xenograft system, in which hpv-11 induced morphological transformation of human foreskin. virus-specific neutralization was demonstrated by the ability of an hpv-11-specific polyclonal antiserum to neutralize hpv-11 infectivity and not bovine papillomavirus type 1 (bpv-1) or cottontail rabbit papillomavirus (crpv) infectivity. in all three virus infectivity systems, neu ... | 1990 | 1693698 |
| human xenografts. a model system for human papillomavirus infection. | epithelial chips of human foreskin or cervix infected in vitro with one strain of human papillomavirus type 11 (hpv-11) and subsequently transplanted to the renal capsule of athymic mice will yield, after 3 months of in vivo incubation, epithelial cysts that are morphologically transformed and appear identical in every way to human condylomata. these cysts synthesize virus rna, dna, proteins, and infectious virions. the cysts can be utilized as a source of virus for continued passage of infectio ... | 1990 | 2165039 |
| production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. | a monoclonal antibody was raised against the major capsid protein l1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the l1 protein, as a target for screening. this antibody, designated camvir-1, reacted with a 56 kilodalton protein in cells infected with l1-vaccinia virus, and the protein was present in a predominantly nuclear location. the antibody also detects the hpv-16 l1 antigen in formalin fixed, paraffin wax embedded biopsy specimens and on routine cerv ... | 1990 | 2166093 |
| a general procedure for evaluation of immunological relevance of synthetic peptides: peptides synthesized on paper in enzyme-linked immunosorbent assay. | multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (whatman 3mm paper). paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. the method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of hiv, the epstein-barr virus-determined nucl ... | 1990 | 1703730 |
| co-transformation by human papillomavirus types 6 and 11. | human papillomavirus (hpv) types 6 and 11 are usually found in benign genital lesions and laryngeal papillomas. however, the occasional occurrence of their dnas in carcinomas of the genital tract and larynx suggests that they have some tumorigenic activity. in this paper, we have examined the cotransforming and transactivation activities of the e7 genes from these virus types and show that they cooperate with ras to transform primary cells, but at a greatly reduced level compared to hpv-16 e7. a ... | 1990 | 2154533 |
| transforming activity of e5a protein of human papillomavirus type 6 in nih 3t3 and c127 cells. | human papillomavirus type 6 (hpv-6) is the etiologic agent of genital warts and recurrent respiratory papillomatosis. we are investigating the mechanism by which this virus stimulates cell proliferation during infection. in this paper, we report that the e5a gene of hpv-6c, an independent isolate of hpv-11, is capable of transforming nih 3t3 cells. the e5a open reading frame (orf) was expressed under the control of the mouse metallothionein promoter in the expression vector pmt.neo.1, which also ... | 1990 | 2161939 |
| prevalence of hpv cervical infection in a family planning clinic determined by polymerase chain reaction and dot blot hybridisation. | the overall prevalence of human papillomavirus (hpv) cervical infection in 131 women attending a family planning clinic was 7% (hpv 6/11, 16, 18, 31) by dot blot hybridisation, 53% (hpv 11, 16, 31) by polymerase chain reaction (pcr), and 56% by the two methods combined. hpv 16 and 18 were the commonest types (4% each) by dot blot, hpv 16 (39%) by pcr. fifteen percent of subjects had mildly abnormal cervical cytology (grades 1a, 2a, or 3). there was no significant correlation between cytological ... | 1991 | 1919537 |
| a key dna-protein interaction determines the function of the 5'urr enhancer in human papillomavirus type 11. | the 5' end of the upstream regulatory region (urr) of human papillomavirus type 11 (hpv-11) has enhancer function and binds cellular proteins. we have determined that one particular motif, a ccngtnac pair, is both necessary and sufficient for enhancer activity. this enhancement of expression can be competed in vivo by concatenated double-stranded oligonucleotides, indicating that protein-dna binding is a requisite for enhancer activity. a 41-kda protein, present in all epithelial cells assayed, ... | 1991 | 1847254 |
| focal epithelial hyperplasia (heck's disease): the first reported case from italy. | a case of focal epithelial hyperplasia (heck's disease) of the oral mucosa observed for the first time in italy is reported. the patient was of italian extraction. the lesions, represented by soft nodules, were multiple and located on the vestibular and labial mucosa. biopsy tissues were studied for the presence of human papilloma virus (hpv) by electron microscopy, in situ hybridization and immunoperoxidase staining to hpv group antigens. no viral particles consistent with hpv were found in the ... | 1991 | 1850180 |
| molecular pathologic study of human papillomavirus infection in inverted papilloma and squamous cell carcinoma of the nasal cavities and paranasal sinuses. | nasal inverted papilloma is a rare benign tumor occasionally associated with squamous cell carcinoma. to determine the etiological role of human papillomavirus in inverted papilloma, and to clarify the relationship between human papillomavirus and malignant transformation of this benign tumor, we retrospectively analyzed inverted papillomas from 26 patients, 7 of whom had squamous cell carcinoma. we used an immunohistochemical method and molecular pathologic techniques, or dot-blot hybridization ... | 1991 | 1845817 |
| the open reading frame l2 of cottontail rabbit papillomavirus contains antibody-inducing neutralizing epitopes. | polyclonal antisera were generated against bacterially derived fusion proteins of the open reading frames (orfs) of the capsid proteins of cottontail rabbit papillomavirus (crpv). the carboxy-terminal two-thirds of crpv l1 and the carboxy-terminal half of crpv l2 were cloned into a bacterial expression vector and induced proteins were used as antigen and immunogen. the polyclonal antisera were tested in a series of immunological assays, including elisa, western blot, and neutralization of crpv. ... | 1991 | 1707567 |
| an e1m--e2c fusion protein encoded by human papillomavirus type 11 is asequence-specific transcription repressor. | we have isolated a putative, spliced e5 cdna of human papillomavirus type 11 (hpv-11) by polymerase chain reaction amplification of cdnas from an experimental condyloma. using retrovirus-mediated gene transfer, we isolated two novel hpv-11 cdnas, one of which had a splice linking nucleotides 1272 and 3377. this transcript also existed in experimental condylomata and in cervical carcinoma cells transfected with cloned genomic hpv-11 dnas. the 5' end of the transcript in transfected cells originat ... | 1991 | 1851879 |
| duplication of the upstream regulatory sequences increases the transformation potential of human papillomavirus type 11. | infections with certain types of papillomaviruses, e.g., human papillomavirus type 16 (hpv-16), often progress to cancer. malignant lesions associated with the closely related hpv-11 are extremely rare. additionally, hpv-11 dna, unlike hpv-16, does not normally transform cells in vitro. we determined that hpv-11 dna was able to transform baby rat kidney cells in a ras-dependent focus assay when the upstream regulatory region (urr) was present in two copies. addition of a second hpv-11 urr or an ... | 1991 | 1656601 |
| the enhancer of human papillomavirus type 16: binding sites for the ubiquitous transcription factors oct-1, nfa, tef-2, nf1, and ap-1 participate in epithelial cell-specific transcription. | the enhancer of human papillomavirus type 16 (hpv-16) is considered to be specific for epithelial cells, in particular for cervical carcinoma-derived cell lines. we reexamined this hypothesis with the complete enhancer as well as nonoverlapping subclones and found all clones to be active in epithelial cell lines derived from the epidermis and from carcinomas of the cervix, mammary gland, and colon, but inactive in fibroblast, lymphoma, and embryonal carcinoma cells. although the virus infects on ... | 1991 | 1656080 |
| detection of human papillomavirus types 6 and 11 e4 gene products in condylomata acuminatum. | polyclonal antiserum to an escherichia coli-produced beta-galactosidase/e4 fusion protein of human papillomavirus type 6b (antiserum 256), and affinity purified hpv 11 anti-e4 antibodies were tested for reactivity in western blots with bacterially expressed trpe/e4 fusion proteins of hpv types 6b, 11, 16, and 18. to further characterize the affinity purified anti-e4 antibodies, a dot-immunobinding assay was performed using overlapping synthetic hpv 11 e1e4 peptides as antigens. protein extracts ... | 1991 | 1653305 |
| human papillomaviruses in the pathogenesis of anogenital cancer. | substantial experimental evidence accumulated over the past 8 years has indicated an etiological role for specific human papillomavirus (hpv) types in anogenital cancer and its premalignant precursors. virus infection and viral gene expression emerge as necessary but obviously not sufficient factors for cancer induction. additional modifications of host cell genes appear to be required for malignant progression of infected cells. the expression of viral oncoproteins in cells infected by "high-ri ... | 1991 | 1651607 |
| sinonasal papillomas and human papillomavirus: human papillomavirus 11 detected in fungiform schneiderian papillomas by in situ hybridization and the polymerase chain reaction. | a series of 19 paraffin-embedded sinonasal papillomas (four squamous papillomas, three fungiform papillomas, nine inverted papillomas, and three cylindrical cell papillomas) were investigated for evidence of human papillomavirus (hpv) infection using immunohistochemistry (polyclonal antibody to hpv capsid antigen), in situ hybridization (dna probes for hpv 6/11, 16/18, and 31/33/35), and the polymerase chain reaction (primers and probes for hpv 6, 11, 16, 18, and 33). all three fungiform papillo ... | 1991 | 1650753 |
| identification of human papillomaviruses in paraffin embedded cervical pathological tissues from indian women by polymerase chain reaction. | the polymerase chain reaction (pcr) technology was used to identify human papillomaviruses (hpv) in 52 paraffin embedded cervical tissues from indian women with chronic cervicitis, different grades of cervical dysplasia and invasive cervical carcinoma. the tissues were screened for amplification of the cellular beta-globin gene as well as of hpvs. sets of primers designed to amplify a portion of the e6 gene of hpv 6, 11, 16, 18, 31, 33 and 35 were employed. hpv 6, 16 and 31 were identified in 58 ... | 1991 | 1648312 |
| the prevalence of human papillomavirus type 16 and 18 dna in cervical cancer in different age groups: a study on the incidental cases of cervical cancer in norway in 1983. | in a study carried out to estimate the prevalence of genital human papilloma virus (hpv) infection among cervical cancer patients in norway, the following values were recorded using the southern blot hybridization method: 54.1% hpv 16, 9.5% hpv 18, 0% hpv 11, and 1.4% hpv 16 and 18 coinfection, yielding a total of 62.2% hpv positive cases in the study. an attempt to include a normal biopsy as "internal control" from each patient failed, as macroscopically normal tissue was often histologically a ... | 1991 | 1646757 |
| use of human papillomavirus type 11 virions in an elisa to detect specific antibodies in humans with condylomata acuminata. | human papillomavirus types 6 and 11 (hpv-6 and hpv-11) are the major aetiological agents of condylomata acuminata. serological studies of this disease have been difficult to perform and interpret because native, type-specific antigens have not been available. in particular, since these viruses have not been propagated in vitro and sufficient quantities of virions are not present in lesions, virus particles have been difficult to obtain. in the present study, we used hpv-11 particles, obtained fr ... | 1991 | 1646276 |
| squamous cell carcinoma of the lung in a nonsmoking, nonirradiated patient with juvenile laryngotracheal papillomatosis. evidence of human papillomavirus-11 dna in both carcinoma and papillomas. | malignant transformation of laryngeal juvenile papillomatosis most often occurs in patients with previous radiation therapy or smoking histories. we report the case of a 35-year-old, nonsmoking, nonirradiated man who developed squamous cell carcinoma of the lung with a 33-year history of laryngotracheal juvenile papillomatosis. postmortem examination showed pulmonary cavitating papillomatosis and chest wall, vertebrae, and peribronchial lymph node involvement by tumor. molecular studies (souther ... | 1991 | 1659237 |
| application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization. | silver-intensification methods described in the literature for the diaminobenzidine (dab) and diaminobenzidine-nickel (dab/ni) endproduct of the peroxidase reaction were compared in model systems after immunoperoxidase and in situ hybridization. first, these methods were compared in immunohistochemical model systems, using the demonstration of glial fibrillar acidic protein (gfap) and prostate-specific antigen (psa) in paraffin sections of human brain and prostate tissue, respectively. when dab ... | 1992 | 1532404 |
| [anal condylomata acuminata in hiv positive patients]. | since july 1986 we started with following all patients with condylomata acuminata including hiv-testing and human papilloma virus (hpv) identification by dna-dna-hybridisation (southern blot). seventy patients are included, 39 of them are seropositive. the ratio male to female is 59 to 11, in seropositives 35 to 4, in negatives 24 to 7. the average age is 28 and 31 years respectively. the number of homosexuals and junkies is significantly higher in seropositives. seventeen patients are in hiv-st ... | 1992 | 1508008 |
| detection of human papillomavirus dna in skin warts from immunocompromised patients but not in semen from men whose wives have abnormal cervical cytology. | the epidemiology of human papillomaviruses (hpv) was studied in 61 immunocompromised patients (e.g. renal and cardiac transplants; bowen's disease; genital cancer) undergoing therapy at the university hospital of wales at cardiff u.k. warts from various sites of these patients were studied for the presence of hpv types 6, 11, 16 and 18 using the dot-blot dna hybridization technique. four hpv-16 and one hpv-11 was detected. the presence of hpv-16 in our study is quite significant since it suggest ... | 1992 | 1338072 |
| an in vitro system for studying the initial stages of cottontail rabbit papillomavirus infection. | cottontail rabbit papillomavirus (crpv) infection of an established cottontail epidermal cell line (sf1ep) resulted in the production of crpv-specific transcripts without concomitant morphological transformation. the most abundant transcripts corresponded in size to those of the e6 and e7 open reading frames (orfs), which are also among the commonest in domestic and cottontail rabbit papillomas. crpv rna production was both time- and dose-dependent, with rna production diminishing with decreasin ... | 1992 | 1331149 |
| cellular transformation by a unique isolate of human papillomavirus type 11. | infection with human papillomavirus type 11 (hpv 11) is associated with benign epithelial proliferations and rarely with malignant and metastasizing tumors. because of the biological diversity displayed in tissues infected with hpv 11, we have examined the capacity of various isolates of hpv 11 to transform cultured cells and compared their molecular differences by dna sequence analysis. five isolates of hpv 11 were examined for their ability to transform primary neonatal rat kidney epithelial c ... | 1992 | 1327518 |
| production of human papillomavirus and modulation of the infectious program in epithelial raft cultures. off. | human papillomaviruses trophic for anogenital epithelia cause benign warts, and certain genotypes are closely associated with cervical neoplasia. by using our modifications of the epithelial raft culture system, we were able to recapitulate and modulate the infectious program of a papillomavirus in vitro for the first time. small pieces of a condyloma containing human papillomavirus type 11 were explanted onto a dermal equivalent consisting of a collagen matrix with fibroblasts and were cultured ... | 1992 | 1321068 |
| detection of herpes simplex virus type-2 dna and human papilloma virus dna sequences in cervical carcinoma tissue by molecular hybridization. | biopsy samples from one hundred and two patients with squamous cell carcinoma of the uterine cervix and tissues from twelve healthy normal cervical tissues (post hysterectomy) were examined for hsv-type 2 and hpv-11 dna sequences by molecular hybridization technique. in the carcinoma tissue extracts 53% contained hsv-2 dna, 27% -hsv-1-dna and 36% showed hpv-11 gene sequences while 5.7% were found to contain both hpv and hsv-2 dna. biopsies from healthy cervix were completely negative for hsv-2 a ... | 1992 | 1320668 |
| predominance of serum antibodies to synthetic peptide stemming from hpv 18 open reading frame e2 in cervical adenocarcinoma. | to determine if there are type specific differences in serum antibody responses to synthetic peptides derived from human papillomavirus (hpv) open reading frame (orf) e2 in patients with cervical carcinoma. | 1992 | 1320634 |
| a multiple luminescent procedure for the detection of different papillomaviruses on dot blots. | we developed a method based on the use of various luminescent systems for identification of several nucleic acid sequences on the same dot blots. in a simultaneous assay, the target dna molecules were hybridized with different dna probes. these probes were labelled with biotin or digoxigenin or directly coupled to enzymes such as glucose-6-phosphate dehydrogenase, peroxidase or alkaline phosphatase. after hybridization, these labels were detected by luminescent reactions using an amplified camer ... | 1992 | 1319441 |
| human papillomavirus type 11 in multiple squamous cell carcinomas in a patient with subacute cutaneous lupus erythematosus. | squamous cell carcinoma (scc) is a rare complication of cutaneous lupus erythematosus (le). aside from the documented role of ultraviolet light, scc may arise in areas of chronic scarring, such as in lesions of discoid le. iatrogenic immunosuppression associated with organ transplantation also results in a predisposition to scc, often with multiple tumors. a role for certain human papillomaviruses (hpv) in the development of squamous cell carcinoma has been documented; specifically, hpv types 5 ... | 1992 | 1319432 |
| replication of plasmid-derived human papillomavirus type 11 dna in cultured keratinocytes. | plasmid-derived human papillomavirus type 11 (hpv11) dna has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. hpv11 dna was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with psv2neo. transfectants were selected and shown to contain replicated episomal hpv dna. once selected, hpv11 dna persists in cells through at least two additional passages. | 1992 | 1313926 |
| intermolecular triplex formation distorts the dna duplex in the regulatory region of human papillomavirus type-11. | a conformational distortion in the dna duplex at the regulatory region of human papillomavirus type-11 next to an intermolecular triplex, formed with a synthetic oligonucleotide, was investigated with several chemical probes. the sequence targeted for triplex formation borders on the binding sites for the regulatory proteins encoded by the viral e2 open reading frame. dimethyl sulfate, diethyl pyrocarbonate, and oso4 all react to a greater extent with nucleotides in the duplex that are immediate ... | 1992 | 1312091 |
| antibody-mediated neutralization of human papillomavirus type 11 (hpv-11) infection in the nude mouse: detection of hpv-11 mrnas. | the nu/nu mouse xenograft is the only experimental system permitting the growth of human papillomaviruses (hpv). previous studies demonstrating inhibition of hpv-11 infection by antibodies against hpv-11 virions have used indirect markers of infection, such as graft size and histopathologic features. the presence of hpv-11 mrnas was used as a direct marker of infection: infectious hpv-11 was incubated with rabbit serum raised against purified hpv-11 virions or with the corresponding preimmune se ... | 1992 | 1309849 |
| detection of human serum antibodies that neutralize infectious human papillomavirus type 11 virions. | a selection of human sera were tested for the presence of antibodies that neutralized infectious human papillomavirus (hpv) type 11. neutralizing antibodies were detected by prevention of hpv-11-induced condylomatous transformation of human foreskin chips transplanted subrenally into athymic mice. test sera were obtained from 21 female patients with genital condylomas and eight patients with laryngeal papillomas. control patients consisted of 57 adult random blood donors and five asymptomatic ch ... | 1992 | 1316943 |
| viral e1 and e2 proteins support replication of homologous and heterologous papillomaviral origins. | we have shown that e1 and e2 proteins of human papillomavirus type 11 (hpv-11) were essential to support the replication of the homologous viral origin (ori) in a transient replication assay, similar to reports on bovine papillomavirus type 1 (bpv-1). unexpectedly, matched or even mixed combinations of e1 and e2 proteins from hpv-11 or bpv-1 replicated either ori in human, monkey, and rodent cell lines of epithelial or fibroblastic lineage, albeit with varied efficiencies. either set of viral pr ... | 1992 | 1321423 |
| control of human papillomavirus type 11 origin of replication by the e2 family of transcription regulatory proteins. | replication of human papillomavirus type 11 (hpv-11) dna requires the full-length viral e1 and e2 proteins (c.-m. chiang, m. ustav, a. stenlund, t. f. ho, t. r. broker, and l. t. chow, proc. natl. acad. sci. usa 89:5799-5803, 1992). using transient transfection of subgenomic hpv dna into hamster cho and human 293 cells, we have localized an origin of replication (ori) to an 80-bp segment in the upstream regulatory region spanning nucleotide 1. it overlaps the e6 promoter region and contains a sh ... | 1992 | 1323690 |
| properties of bovine papillomavirus e1 mutants. | ostensibly comparable mutants of bovine papillomavirus type 1 (bpv-1) affecting the e1 open reading frame that were constructed in several laboratories have been reported to exhibit either reduced or increased transformation efficiencies in established mouse cell lines relative to wild-type bpv-1 dna. to resolve these discrepancies, we have reexamined many of the mutants in mouse c127 cells by using focus formation assays. our primary conclusions are that all e1 mutants tested consistently gener ... | 1992 | 1333131 |
| serological assays for the detection of hpv antibodies. | a variety of serological assays to detect antibodies to genital-type hpvs have been developed. bacterially expressed fusion proteins, synthetic peptides and hpv 11 virus propagated in a xenograft system have been the most commonly used antigen targets in either western blot assays or elisas. hpv antibodies have been readily detected and most studies suggest that they are type-specific. primarily, antibodies appear to be directed against the capsid antigens. the presence or titre of antibodies to ... | 1992 | 1330907 |
| monoclonal antibody neutralization of bpv-1. | mouse monoclonal antibodies were generated against intact infectious bpv-1 virions by methods previously described (christensen et al., 1990). elisa was used to screen for reactivities to intact and/or disrupted bpv-1, crpv and hpv-11 virions. several hybridomas were initially selected that showed antibody reactivity by elisa to both intact and disrupted bpv-1, to disrupted bpv-1 only, or to intact bpv-1 virions. one monoclonal antibody, designated b1.a1, which reacted only to intact bpv-1 was s ... | 1993 | 7686316 |
| propagation of human papillomavirus type 11 in human xenografts using the severe combined immunodeficiency (scid) mouse and comparison to the nude mouse model. | we report propagation of human papillomavirus (hpv) type 11 in human xenografts in the severe combined immunodeficiency (scid) mouse and compare this new animal model for hpv infection to the previously established athymic nude mouse model. hpv-11-infected foreskin fragments grafted under the renal capsule of scid mice formed large epithelial cysts that had the histologic and immunocytochemical features of hpv infection. this infection was successfully passaged to nude mice. viral particles that ... | 1993 | 8212584 |
| laryngeal papilloma cells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation. | laryngeal papillomas are benign epithelial tumors caused by human papillomaviruses. these tumors are characterized by hyperplasia of the spinous layer and abnormal differentiation. many tumor cell lines over-express the epidermal growth factor (egf) receptor on their surface, and egf regulates normal cell growth. we have asked about the relationship of the egf receptor and egf response in laryngeal papilloma cells. papilloma cells showed markedly greater immunohistochemical staining for the egf ... | 1993 | 7679053 |
| two e2 binding sites (e2bs) alone or one e2bs plus an a/t-rich region are minimal requirements for the replication of the human papillomavirus type 11 origin. | human papillomaviruses (hpvs) cannot be propagated in vitro, but the dna can be replicated transiently in an assay in the presence of two trans-acting viral proteins, e1 and e2. using this assay, we have defined the minimal cis-acting elements of the origin of replication of hpv type 11. most hpv genomes are conserved at the origin of replication, and the core contains three e2 binding sites (e2bs) surrounding an a/t-rich spacer region. the present results show that the minimal requirement for r ... | 1993 | 8230435 |
| regulation of the human papillomavirus type 11 e6 promoter by viral and host transcription factors in primary human keratinocytes. | human papillomavirus (hpv) type 11 is strictly trophic for epithelial cells and induces benign condylomata of the external genitalia and also causes laryngeal papillomas. primary keratinocytes are the appropriate hosts for studies of hpv gene regulation, but they are not frequently used, owing to difficulties in culturing and low transfection efficiencies. by modifying a polybrene transfection procedure, we achieved consistently high transfection efficiencies in primary human foreskin keratinocy ... | 1993 | 8382318 |
| in vitro evaluation of phosphorothioate oligonucleotides targeted to the e2 mrna of papillomavirus: potential treatment for genital warts. | papillomaviruses induce benign proliferative lesions, such as genital warts, in humans. the e2 gene product is thought to play a major role in the regulation of viral transcription and dna replication and may represent a rational target for an antisense oligonucleotide drug action. phosphorothioate oligonucleotides complementary to e2 mrnas were synthesized and tested in a series of in vitro bovine papillomavirus (bpv) and human papillomavirus (hpv) models for the ability to inhibit e2 transacti ... | 1993 | 8383937 |
| characterization of human papillomavirus type 11 e1 and e2 proteins expressed in insect cells. | the study of human papillomavirus replication has been hampered by the lack of an in vitro system which reliably supports virus replication. recent results from the bovine papillomavirus (bpv) system indicate that the e1 and e2 proteins are the only viral gene products required for replication. by analogy with simian virus 40 large t antigen, e1 is thought to possess atpase and helicase activity, which may play a direct role in viral dna replication. the precise role of e2 is unclear, but it may ... | 1993 | 8386271 |
| [human papillomavirus-dna in laryngeal papilloma detected by nucleic acid hybridization and the clinical treatment]. | we examined 16 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their diseases, for the presence of human papilloma virus (hpv). biopsies were obtained from the papillomas site of larynx or trachea. the hpv-dna 6b, 11, 16 were determined by using slot blot hybridization technique, type hpv-6b positive 87.5%, hpv-11 positive 93.7% hpv-16 positive 81.6%. using southern blot procedure examined 12 hpv-dna positive samples, the results show that 3 were ty ... | 1993 | 8031590 |