Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| focal epithelial hyperplasia. | in 2 children (brother and sister) focal epithelial hyperplasia could be diagnosed by means of histology, human papillomavirus typing and southern blot. in the 14-year-old girl the history could be followed over many years. the high familial incidence and the special geographic distribution of focal epithelial hyperplasia are pointed out. | 1992 | 1498399 |
| [anal condylomata acuminata in hiv positive patients]. | since july 1986 we started with following all patients with condylomata acuminata including hiv-testing and human papilloma virus (hpv) identification by dna-dna-hybridisation (southern blot). seventy patients are included, 39 of them are seropositive. the ratio male to female is 59 to 11, in seropositives 35 to 4, in negatives 24 to 7. the average age is 28 and 31 years respectively. the number of homosexuals and junkies is significantly higher in seropositives. seventeen patients are in hiv-st ... | 1992 | 1508008 |
| laser treatment of premalignant and malignant squamous cell lesions of the penis. | thirty men with biopsy-proven premalignant or malignant squamous cell lesions of the penis were treated. all had subclinical aceto-white lesions with histologic evidence for human papilloma virus infection. nineteen patients had penile intraepithelial neoplasia (pin i and ii) and 11 had squamous cell carcinoma. of these 11 patients, 6 had noninvasive penile intraepithelial neoplasia--carcinoma in situ (pin iii/tis)--and 5 had invasive squamous cell carcinoma (4 stage t2 and 1 t3). all were treat ... | 1992 | 1508018 |
| signal transduction cascade shared by epidermal growth factor and platelet-derived growth factor is a major pathway for oncogenic transformation in nrk cells. | we have isolated two recessive, mutually complementary nrk cell mutants that are refractory to transformation by epidermal growth factor (egf) and transforming growth factor-beta. both mutants are defective in a signal transduction cascade shared by egf and platelet-derived growth factor (pdgf). analysis of the mutants suggests that transformation of nrk cells by the v-fms, v-erbb, activated erbb-2, v-ras, v-fos, v-mos, v-fes, v-src, sv40 large t, polyomavirus middle t, and human papillomavirus ... | 1992 | 1515413 |
| prognostic factors in cervical human papillomavirus infections. | a prospective follow-up study, without further treatment, of a series of 528 women with cervical human papillomavirus (hpv) infections was conducted from 1981 to the present, with a mean follow-up time of 60.3 months (standard deviation 25.1 months). the women visited the outpatient department of gynecology, kuopio university hospital, kuopio, finland at six-month intervals. at each visit, a thorough gynecologic examination, pap-smear, and colposcopy with or without punch biopsy were performed. ... | 1992 | 1523531 |
| application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization. | silver-intensification methods described in the literature for the diaminobenzidine (dab) and diaminobenzidine-nickel (dab/ni) endproduct of the peroxidase reaction were compared in model systems after immunoperoxidase and in situ hybridization. first, these methods were compared in immunohistochemical model systems, using the demonstration of glial fibrillar acidic protein (gfap) and prostate-specific antigen (psa) in paraffin sections of human brain and prostate tissue, respectively. when dab ... | 1992 | 1532404 |
| tissue restricted expression and chromosomal localization of the yb-1 gene encoding a 42 kd nuclear ccaat binding protein. | yb-1 cdna clones were isolated by binding site screening of a hela expression library using a human papillomavirus type 18 enhancer oligonucleotide. yb-1 belongs to a family of transcription factors which bind to recognition sequences containing a core ccaat element. yb-1 bound to its single stranded recognition sequence on the sense strand but not to the anti-sense strand. a synthetic peptide antiserum derived from the predicted yb-1 amino acid sequence identified a 42 kd nuclear protein in imm ... | 1992 | 1542575 |
| ten year follow-up study of women presenting to a genitourinary medicine clinic with anogenital warts. | one hundred heterosexual women presenting at our clinic in 1979 with anogenital warts, were reviewed 10 years later. median duration of warts following initial clinic attendance was 2 months (range 0-120 months). in 1979 cervical pap smear results were available for 76 patients; cervical intraepithelial neoplasia (cin) was seen in 15/76 (19.7%) women; 3 (4%) women had low grade cin, 12 (15.7%) women had high grade cin. nineteen women had had treatment for cin between 1979 and 1989, 7 laser ablat ... | 1992 | 1543764 |
| hpv16 e7 phosphorylation in fission yeast: characterization and biological effects. | human papillomavirus type 16 e7 protein (hpv-16 e7), synthesised in schizosaccharomyces pombe, is both phosphorylated and targeted to the nucleus [tommasino et al., gene 93 (1990) 265-270] as is e7 protein synthesized in primate cells. further analysis of e7 expression in fission yeast indicates that: (i) e7 protein synthesised in s. pombe is phosphorylated only on the ser residues which are part of a casein kinase ii consensus site, as it has been shown to be the cause in human cells, and is ti ... | 1992 | 1547958 |
| characterization of functional hpv-16 e7 protein produced in escherichia coli. | human papillomaviruses (hpvs) are the etiologic agents responsible for genital warts and are contributing factors in the pathogenesis of human cervical cancer. the hpv e7 gene is transcriptionally active in these diseases and has been shown to transform mammalian cells in vitro. we have expressed and purified the hpv-16 e7 gene product in escherichia coli. the isolated e7 protein contains zinc in a 1:1 molar ratio. x-ray absorption fine structure studies demonstrated that the zinc is coordinated ... | 1992 | 1551900 |
| association between hiv infection and cervical neoplasia: implications for clinical care of women at risk for both conditions. | both aids and cervical neoplasia (cn) can result from sexual transmission of hiv infection and may affect similar groups of women. available data on the association between aids and cn have practical implications for gynecological care. we review these data to provide an estimate of the magnitude of the association between cn and hiv infection. | 1992 | 1558715 |
| retinoic acid and calcitriol inhibition of growth and nadh oxidase of normal and immortalized human keratinocytes. | plasma membranes were isolated by aqueous two-phase partition from normal human keratinocytes (hkc) and from human keratinocytes immortalized with human papillomavirus type 16 dna (hkc/hpv16). the nadh oxidase of plasma membrane vesicles of normal hkc was stimulated by epidermal growth factor whereas that of hkc/hpv16 was not. the nadh oxidase of the plasma membranes from both normal hkc and hkc/hpv16 was inhibited by calcitriol (1 alpha-1,25-dihydroxy vitamin d-3) and retinoic acid. however, wi ... | 1992 | 1558845 |
| delivery and expression of a heterologous antigen on the surface of streptococci. | we have developed a system in which a foreign antigen replaces nearly all of the surface-exposed region of the fibrillar m protein from streptococcus pyogenes and is fused to the c-terminal attachment motif of the m molecule. the fusion protein is thus expressed on the surface of streptococcus gordonii, a commensal organism of the oral cavity. the antigen chosen to be expressed within the context of the m6 molecule was the e7 protein (98 amino acids) of human papillomavirus type 16. stable recom ... | 1992 | 1563781 |
| schneiderian papilloma of the nose and paranasal sinuses: the university of ottawa experience. | schneiderian papillomata of the nose and the paranasal sinuses are uncommon neoplasms. although histologically benign, malignancy can occur. the likely etiologic agent is the human papilloma virus. we reviewed the cases treated at university of ottawa-affiliated hospitals from 1969-1989. there were 35 patients consisting of 26 men and nine women with an average age of 55.8 years. the most common presenting symptom was unilateral nasal obstruction (71.4%). this was caused in most cases by an ipsi ... | 1992 | 1564756 |
| predictors of abnormal cervical cytology: statistical analysis of human papillomavirus and cofactors. | reported increases in the incidence of human papillomavirus infection have generated speculation that cervical cytological abnormalities may also be increasing. a 10-year retrospective study of cervical cytology in 2,919 college women demonstrated a significant increase in the frequency and severity of cervical-cytological abnormalities (p - 0.0001). the peak year for abnormal cervical cytology coincided with the peak year for overt human papillomavirus infection. women with papillomarivus, geni ... | 1992 | 1565301 |
| cervical cancer screening and detection of genital hpv-infection and chlamydial infection by pcr in different groups of kenyan women. | a pilot study was conducted in nairobi, kenya, to evaluate the feasibility of a larger study on the prevalence of cervical cancer and precancerous lesions and on the role of sexually transmitted diseases (std) as risk factors for cervical cancer in kenya. 692 women were screened for cervical cancer by cytology in 3 city commission family planning clinics and in 1 city commission std referral clinic in nairobi. in one family planning clinic and in the std clinic, a sample of the participating wom ... | 1992 | 1567270 |
| cone biopsy: a review of 112 cases. | a chart review of 112 patients who underwent cold knife conisation was performed. records showed that 73.5% of the patients smoked cigarettes and 26.5% were using oral contraception. in 85.7% of cases pre-operative cytology/colposcopy findings were within one grade of the cone histology. the majority of lesions were cin iii (59.9%). only 4.5% were normal. human papilloma virus infection was detected histologically in 26.8% of patients. pre-operative punch biopsy was undertaken in only five cases ... | 1992 | 1568845 |
| purification and characterization of a functionally homogeneous 60-kda species of the retinoblastoma gene product. | the retinoblastoma susceptibility gene (rb) encodes a 928-amino acid protein (prb) that is hypothesized to function in a pathway that restricts cell proliferation. the immortalizing proteins from three distinct dna tumor viruses (sv40 large t antigen, adenovirus e1a, and human papilloma virus type 16 e7) have been shown to interact with rb protein through two noncontiguous regions comprised of amino acids 393-572 (domain a) and 646-772 (domain b). we constructed a truncated form of rb (rb p60) t ... | 1992 | 1569054 |
| seventh iuvdt regional conference on sexually transmitted diseases, kuala lumpur, 5-7 september 1991. | in september 1991, the 7th iuvdt regional conference on sexually transmitted diseases (stds) convened in kuala lumpur, malaysia, to exchange information on the importance of controlling stds and hiv-aids in asia. speakers from thailand, malaysia, and japan provided the latest hiv-aids epidemiological data. in thailand, heterosexual transmission of hiv is catching up with iv drug use. most infected women are 15-24 years old. in malaysia, drug use iv drug use trails heterosexual transmission o ... | 1992 | 1582659 |
| basal cell and squamous cell carcinoma and kaposi's sarcoma. | the mortality rate of nonmelanoma skin cancer is higher than generally considered. an actual nonmelanoma skin cancer is a risk factor not only for other skin cancers but also for cancers in other organs. the recurrence rate can, according to the method of calculation, yield surprisingly diverging results. statistical mapping of subclinical tumor growth in basal cell carcinoma supplies the margins for tumor-free excision. an even better but more expensive tool for therapy planning is tumor imagin ... | 1992 | 1591311 |
| in situ and invasive vulvar cancer incidence trends (1973 to 1987). | the objective of this study was to examine recent trends in the incidence of vulvar cancer. | 1992 | 1595803 |
| the epidemiology, clinical manifestations and health-maintenance needs of women infected with hiv. | the growing epidemic of hiv infection poses a serious threat to women's health in the united states and abroad. in the united states, hiv disproportionately affects african-american and hispanic-american woman. primary care providers will be faced with an increasing need to provide counseling and clinical services to these women. it is not yet clear whether gender affects the natural history of hiv infection; differences in survival rates may reflect lack of access to care rather than true biolo ... | 1992 | 1603441 |
| vulvodynia. diagnostic patterns. | the symptom of vulvar burning demands special evaluation and management. possible factors include complications of therapy, inflammatory dermatoses, lichen sclerosus, and infection with organisms such as candida, human papillomavirus, and herpes simplex virus. vulvar vestibulitis and dysesthetic vulvodynia or pudendal neuralgia have characteristic patterns of discomfort. psychological aspects of vulvodynia include the need for supportive therapy. | 1992 | 1606768 |
| range of hpv 16 e7 antibodies in cervical cancer patients and healthy subjects. | 1992 | 1612789 | |
| transforming growth factor beta 1 regulation of c-myc expression, prb phosphorylation, and cell cycle progression in keratinocytes. | transforming growth factor beta 1 (tgf-beta 1) is a potent inhibitor of cellular proliferation in a variety of cell types, including skin keratinocytes. tgf-beta 1 suppression of c-myc transcription has been implicated in the mechanism of tgf-beta 1 inhibition of keratinocytes, and evidence suggests that the protein product of the retinoblastoma gene (prb) is a necessary component in this pathway. following growth factor stimulation of quiescent keratinocytes, tgf-beta 1 can inhibit cell cycle p ... | 1992 | 1633111 |
| [laser co2: results of treatment of hpv lesions in male the partner]. | hundred-fifty-seven male partners of patients affected by human papillomavirus (hpv) lesions, underwent co2 laser vaporization between january 1989 and january 1990. the age ranged between 18 and 45 (median 28.2). the vaporization was performed with a coherent system 451 co2 laser equipment, at a power of 16 watt in continuous mode, depth of 2 mm and a free margin of 2 mm. first laser treatment proved successful in 129 patients (82.1%); 17 out of 28 patients with persistent or relapsed pathology ... | 1992 | 1635650 |
| high rates of atypical cervical cytology: occurrence and clinical significance. | the current literature suggests that atypical (benign atypia or class ii) papanicolaou smears occur at a frequency of 1.6-5.4% and are associated with a substantial rate of underlying cervical dysplasia. however, we noted that our rate of atypia exceeded these figures, particularly in our clinic population. to quantify this observation, 1107 consecutive papanicolaou smears from the clinic population were audited prospectively and compared with 262 consecutive papanicolaou smears performed contem ... | 1992 | 1635730 |
| tgf-beta regulation of epithelial cell proliferation. | the predominant effect of tgf-beta 1 on cell proliferation is inhibition. earlier studies demonstrated that tgf-beta 1 inhibition of skin keratinocyte proliferation involves suppression of c-myc transcription and indirect evidence suggested that the protein product of the retinoblastoma gene (prb) may be involved in this process. skin keratinocytes transformed by sv40 and human papilloma virus-16 (hpv-16) or hpv-18 resisted growth inhibition and suppression of c-myc mrna by tgf-beta. transient e ... | 1992 | 1637556 |
| increased e6/e7 transcription in hpv 18-immortalized human keratinocytes results from inactivation of e2 and additional cellular events. | we characterized the state of the viral genome and transcription of human papillomavirus 18 oncogenes, e6 and e7, in immortalized human keratinocytes. at passage 9 after transfection with hpv 18 a homogeneous population of immortal clones was present. these cells have the viral dna integrated within the e2 orf, accompanied by its partial deletion, similarly to what has been found in cervical carcinoma specimens. transcription of the e6 and e7 oncogenes is mediated by the major viral early promot ... | 1992 | 1641976 |
| in vitro expressed hpv 8 e6 protein does not bind p53. | the oncogene e6 of human papillomavirus 8, which is associated with skin cancers in epidermodysplasia verruciformis, was transcribed and translated in vitro. the resulting 17 kda protein did not bind to the cellular p53 in contrast to e6 of hpv16. | 1992 | 1642560 |
| clinical and virological associations between external anogenital warts and cervical hpv infection in an std clinic population. | to determine the significance of overt anogenital warts as indicators of human papillomavirus (hpv) infection of the cervix, 177 women attending a sydney std clinic were screened for evidence of cervical hpv infection using clinical criteria together with cytology and hpv dna dot hybridization. hpv dna probing was also performed on biopsies of 50 exophytic warts. a very high prevalence of both anogenital warts (40%), and of cervical hpv infection (58%) was indicated in this group of women. in th ... | 1991 | 1645205 |
| subclinical human papillomavirus infection of the male lower genital tract: colposcopy, histology and dna analysis. | in 25 partners of women with genital human papillomavirus (hpv) infection or cervical intraepithelial neoplasia, colposcopic examination revealed the existence of subclinical hpv infection of the male lower genital tract in 22 cases. it manifested either as short papillae tipped with acetowhite changes, or flat acetowhite lesions on the foreskin, glans, periurethral region, scrotum, perineum and/or perianal region. multiple lesions involving several anogenital areas were common. some of these ab ... | 1991 | 1645206 |
| human papillomavirus type-related dna and c-myc oncogene alterations in colon cancer cell lines. | although squamous-cell epithelium is the most frequent target site of human papillomavirus (hpv) infection, a similar infection is demonstrated in columnar epithelial cells in this paper. the papillomavirus expression in three cell lines was detected in colorectal adenocarcinoma of chinese patients. the hpv-16 and hpv-18 dna sequences were found in colorectal cancer cell lines, which might suggest the correlation of hpv to the etiology of colorectal cancers. in addition, c-myc oncogene was ident ... | 1991 | 1645245 |
| failure to detect human papillomavirus in solar keratoses of australian patients. | 1991 | 1645330 | |
| comparison of southern blot hybridization and polymerase chain reaction methods for the detection of human papillomavirus dna. | a methodologic study was performed to compare the polymerase chain reaction (pcr) and southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (hpv) infection. three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by cervicovaginal lavage. the study population included 32 women with condylomatous atypia or cervical intraepithelial neoplasia and 88 control women with no known history of cervical n ... | 1991 | 1645370 |
| human papillomavirus type 16 dna in an odontogenic keratocyst. | dna was extracted from an odontogenic keratocyst and assayed for the presence of human papilloma virus (hpv) type 16 dna sequences using high stringency southern blot hybridization. hpv type 16 homologous dna sequences were detected at a copy number of 50-100 genome copies per diploid cell. the oral hpv dna was not identical to the prototype hpv 16 when cleaved with the restriction enzyme pst-i, since it appeared to lack the pst-i c fragment (l2/l1 orfs) and contained "off-sized" high molecular ... | 1991 | 1645405 |
| functional analysis of e2-mediated repression of the hpv18 p105 promoter. | transcription of the transforming genes e6 and e7 of the human papillomavirus type 18 (hpv18) can be repressed by the product of the e2 open reading frame. mutations were introduced in the cis-responsive elements upstream from the e6 and e7 transcriptional promoter, p105. the effect of these mutations in the absence or presence of e2 was examined in the human cervical carcinoma cell line c33, transfected with expression plasmids. in the presence of hpv18 e2, repression was relieved only when bot ... | 1991 | 1645591 |
| difference in prevalence of human papillomavirus genotypes in cytomorphologically normal cervical smears is associated with a history of cervical intraepithelial neoplasia. | the prevalence of human papillomavirus (hpv) genotypes was investigated by the polymerase chain reaction (pcr) method in cytologically normal and abnormal cervical scrapes obtained from asymptomatic women (n = 1,346), participating in a triennial screening program for cervical cancer, and from a gynecological outpatient population (n = 593). in the symptom-free population oncogenic hpv types 16, 18, 31 and 33 were present in 1.5% of cytologically normal scrapes, while the overall hpv prevalence ... | 1991 | 1645699 |
| characterization of in vivo expression of the human papillomavirus type 16 e4 protein in cervical biopsy tissues. | the role of human papillomavirus (hpv) proteins in the pathogenesis of cervical intra-epithelial neoplasia (cin) and invasive cervical cancer is poorly understood. to characterize e4 protein expression in 49 paraffin-embedded cervical biopsies representing different histopathologic grades of disease, antibodies were elicited to a synthetic peptide corresponding to amino acids 20-34 of a protein predicted to be encoded by the hpv 16 e4 open reading frame. the e4 protein was detected throughout th ... | 1991 | 1645754 |
| laryngeal carcinoma associated with human papillomavirus type 16. | the advent of molecular hybridization technology has provided evidence supporting human papillomavirus as an etiologic agent of laryngeal carcinoma. using southern blot analysis, we identified human papillomavirus type 16 dna associated with an invasive laryngeal carcinoma. the virus genome did not appear to be integrated into the host genome, as is often the case with anogenital tumors. laryngeal carcinoma usually arises on the true vocal cords of individuals who demonstrate demographic and lif ... | 1991 | 1645775 |
| biochemical and biological differences between e7 oncoproteins of the high- and low-risk human papillomavirus types are determined by amino-terminal sequences. | differences in the biological characteristics of the high-risk human papillomavirus type 16 (hpv-16) and the low-risk hpv-6 e7 proteins were analyzed and shown to correlate with certain biochemical properties. to ascertain which region of e7 conferred these properties, chimeric e7 genes were constructed by the exchange of the amino and carboxyl coding halves of the hpv-6 and hpv-16 e7 genes. the amino-terminal half of e7 determined the affinity for binding to the retinoblastoma protein prb, the ... | 1991 | 1645802 |
| delineation of human papillomavirus type 18 enhancer binding proteins: the intracellular distribution of a novel octamer binding protein p92 is cell cycle regulated. | the enhancer of human papillomavirus type 18 consists of two functionally redundant domains, one is partially conserved between hpv18 and hpv16, both mediate strong transcriptional enhancement. in contrast, short fragments of the enhancer mediate low transcriptional enhancement, suggesting that there is functional cooperation between hpv enhancer binding factors. previously interactions of the enhancer with nf-1, ap1 and steroid receptors were shown by emsa. here we show by binding site blotting ... | 1991 | 1645869 |
| nucleotide sequence of human papillomavirus (hpv) type 41: an unusual hpv type without a typical e2 binding site consensus sequence. | the complete nucleotide sequence of human papillomavirus type 41 (hpv-41) has been determined. hpv-41 was originally isolated from a facial wart, but its dna has subsequently been detected in some skin carcinomas and premalignant keratoses (grimmel et al., int. j. cancer, 1988, 41, 5-9; de villiers, grimmel and neumann, unpublished results). the analysis of the cloned hpv-41 nucleic acid reveals that its genome organisation is characteristic as for other papillomavirus types. yet, the analysis i ... | 1991 | 1645904 |
| identification of human papillomavirus antigen in a bladder tumor. | human papillomavirus (hpv) antigen was found in one of six noninfiltrating (grade ii) carcinomas of the bladder. the antigen was located in the nuclei of the superficial cells. this virus-bearing tumor occurred in a patient with no known risk factors. the presence of hpv in low-grade urinary lesions seems to be frequent and may reflect the early stages of carcinogenesis; in fact, hpv infections may cause papillomas and carcinomas of the urothelial mucosae as well as the genital mucosae. | 1991 | 1645921 |
| penile human papillomavirus carriage among men attending swedish std clinics. | using the polymerase chain reaction (pcr) technique, we found that up to 84% of 228 men consecutively attending two swedish std clinics harboured human papillomavirus (hpv) types 6 or 16. mean age of the patients was 28 years (range 17-58); 70% were between 17 and 30 years old. among men without a previous history of, or contact with, condylomata the frequency of hpv 6 detection was 76% using the pcr/dot blot (db) analysis and 43% with the pcr/agarose gel (ag) technique. the corresponding figure ... | 1991 | 1646035 |
| a study to estimate the prevalence of upper respiratory tract papillomatosis in patients with genital warts. | respiratory tract papillomas are associated with human papillomavirus (hpv) types 6 and 11; these hpv types are also commonly associated with genital warts (condyloma acuminata). although most commonly seen in young children, the incidence of respiratory tract papillomas in young adults is increasing. it has been postulated that orogenital contact is the means of transmission in this age group. we performed a survey of adults with genital warts to assess the prevalence of respiratory tract papil ... | 1991 | 1646036 |
| cloning and characterization of human papillomavirus type 52 from cervical carcinoma in indonesia. | human papillomavirus (hpv) type 52 from a cervical carcinoma in indonesia was molecularly cloned and characterized. by hybridization with cervical carcinoma dnas from indonesian patients, hpv 52 was detected in 3 of 52 cases (6%), whereas hpv 16 and 18 were detected in 8 and 7 cases, respectively (15% and 13%). sequence analysis revealed that the e6-e7 orfs contained several dna binding motifs (cys-x-x-cys) like previously sequenced hpvs. the e6 orf also contained splice donor and acceptor signa ... | 1991 | 1646174 |
| estrogen and progesterone receptors in cervical human papillomavirus related lesions. | according to recent studies showing that human papillomavirus (hpv) infections can be influenced by sex steroid hormones, we performed estrogen (er) and progesterone (pgr) receptor assays in fresh frozen biopsies of genital-hpv-related lesions. seventy-three women with normal cervix, condyloma, low- and high-grade cin and squamous carcinoma were evaluated in comparison with 15 persons with vulvar and 9 with penile papillomavirus-associated lesions. hpv genotypes were determined by dot-blot hybri ... | 1991 | 1646176 |
| chromosomal insertion and amplification of human papillomavirus 16 dna sequences in a cell line of argyrophil small cell carcinoma of the uterine cervix. | the chromosomal location of human papillomavirus (hpv) 16 dna sequences integrated in a cell line derived from argyrophil small cell carcinoma of the uterine cervix was determined by means of fluorescence in situ hybridization (fish). the hpv 16 dna sequences were integrated near a fragile site and the location of the c-myc oncogene at 8q24.1. amplification of the integrated viral sequences resulted in an abnormally banded region. the amplified hpv 16 dna sequences were also detected in every in ... | 1991 | 1646197 |
| in situ human papillomavirus (hpv) genotyping of cervical intraepithelial neoplasia in south african and british patients: evidence for putative hpv integration in vivo. | in south africa asymptomatic wart virus infection diagnosed by morphological criteria occurs in 16-20% of all ethnic groups; the incidence in black women is 66%. to identify human papillomavirus (hpv) types the prevalence of hpv in cervical intraepithelial neoplasia (cin) in south african women (n = 72) with age matched british women (n = 73) was compared by non-isotopic in situ hybridisation (nish) using digoxigenin labelled probes for hpv 6, 11, 16, 18, 31, 33 and 35 on archival biopsy specime ... | 1991 | 1646237 |
| use of human papillomavirus type 11 virions in an elisa to detect specific antibodies in humans with condylomata acuminata. | human papillomavirus types 6 and 11 (hpv-6 and hpv-11) are the major aetiological agents of condylomata acuminata. serological studies of this disease have been difficult to perform and interpret because native, type-specific antigens have not been available. in particular, since these viruses have not been propagated in vitro and sufficient quantities of virions are not present in lesions, virus particles have been difficult to obtain. in the present study, we used hpv-11 particles, obtained fr ... | 1991 | 1646276 |
| should cervical cytologic testing be augmented by cervicography or human papillomavirus deoxyribonucleic acid detection? | criticism of the papanicolaou smear in the lay press and recent federal legislation regulating cytology laboratories indicate a need to reappraise cervical cancer screening programs. this study directly compares three potential screening tests, used alone or in combination. a total of 1012 women aged 18 to 35 years were screened by cytologic testing, cervicography, and hybridization for human papillomavirus deoxyribonucleic acid, with discrepancies being referred by the last two authors. after f ... | 1991 | 1646568 |
| vulvar vestibulitis: prevalence and historic features in a general gynecologic practice population. | all gynecologic patients seen by the author during a 6-month period were questioned and examined by means of a swab test to determine the prevalence of vulvar vestibulitis and the normal variation in sensitivity of vestibular skin. of 210 patients, 78 (37%) had some degree of positive testing. a total of 31 patients (15%) were found to fulfill the definition of vulvar vestibulitis. a questionnaire was administered to these patients as well as to seven patients in whom vestibulitis had been previ ... | 1991 | 1646569 |
| papanicolaou test and enzyme-linked in-situ hybridization. a combined diagnostic system for papilloma virus infections with high prognostic value. | a combined diagnostic system for human papilloma virus (hpv) infections comprising the papanicolaou test and in-situ hybridization assay was evaluated. cervical smears from 259 women obtained with a "cytobrush" were screened. human papilloma virus genotypes 6/11, 16/18, 31/35/51 were detected by biotin in-situ hybridization in conjunction with a streptavidin-alkaline phosphatase detection complex. the diagnostic sensitivity of this assay was tested by human papilloma virus-dna-positive human cer ... | 1991 | 1646654 |
| reliability of in-situ hybridization of smears and biopsies for papilloma virus genotyping of the uterine cervix. | two sampling methods, biopsy and cell smear, were investigated for their reliability in the biotin in-situ hybridization test for human papilloma virus deoxyribonucleic acid (hpv-dna)-6/11 and 16/18. cervical smears and biopsies were obtained simultaneously from 81 women with cervical lesions. the sensitivity of the in-situ hybridization was tested on human cervical carcinoma cell lines. as a reference method, hpv-dna was probed in biopsies using a southern blot with 32p-labelled dna probes. thi ... | 1991 | 1646655 |
| immunosuppression and human genital papillomavirus infection. | a group of 39 patients who underwent iatrogenic immunosuppression for renal transplant were evaluated by means of colposcopic, cytologic, and histologic investigations. evidence of human papillomavirus (hpv) infection was found in 30.7%, more frequently in the cervix. biopsy specimens of the most atypical areas showed the presence of cervical intra-epithelial neoplasia in one case. the high incidence of hpv infection in this group suggests that immunodeficient patients should have close monitori ... | 1991 | 1646723 |
| identification of human papillomavirus type 16 in primary and recurrent cervical cancer following radiation therapy. | formalin-fixed, paraffin-embedded tissue blocks from 13 women with cervical carcinoma that recurred following radiation therapy were evaluated for the presence of human papillomavirus (hpv) by in situ hybridization using ribonucleic acid 35s-labeled probes for hpv types 6, 11, 16, and 18. ten of thirteen patients also had pretreatment biopsies from their primary tumors available for analysis. hpv 16 was detected in both primary and recurrent lesions in 4 women. in 1 case, hpv was detected in the ... | 1991 | 1646755 |
| detection of human papillomavirus genotypes in cervical neoplasia from korean women using polymerase chain reaction. | the histopathologic features of cervical dysplasia (20 cases) and cervical carcinoma (15 cases) in korean women were correlated with the presence of human papillomaviruses (hpvs) as determined by polymerase chain reaction technology on paraffin-processed specimens. a segment of the e6 open reading frame of several hpv types (hpv 6, 11, 16, 18, 31, 33, and 35) was amplified using primers which were synthesized to contain 50% g + c, in order to give optimum annealing for the amplification of hpvs. ... | 1991 | 1646756 |
| the prevalence of human papillomavirus type 16 and 18 dna in cervical cancer in different age groups: a study on the incidental cases of cervical cancer in norway in 1983. | in a study carried out to estimate the prevalence of genital human papilloma virus (hpv) infection among cervical cancer patients in norway, the following values were recorded using the southern blot hybridization method: 54.1% hpv 16, 9.5% hpv 18, 0% hpv 11, and 1.4% hpv 16 and 18 coinfection, yielding a total of 62.2% hpv positive cases in the study. an attempt to include a normal biopsy as "internal control" from each patient failed, as macroscopically normal tissue was often histologically a ... | 1991 | 1646757 |
| viral transcription in human keratinocyte cell lines immortalized by human papillomavirus type-16. | human papillomavirus type-16 (hpv-16) transcription in two human keratinocyte cell lines (hpk) immortalized by transfection of viral dna in vitro was analyzed by nucleotide sequencing of cdna clones, and in addition by primer extension analysis and s1 nuclease and exonuclease vii digestion of poly(a)+ rna. a novel mrna species which probably initiates in the e7 orf and in which the 5'-part of the e1 orf (splice donor at position (pos.) 880) is joined to an exon comprising the entire e2 orf (spli ... | 1991 | 1647072 |
| [epidemiologic background and changes in patients infected with human papilloma virus]. | contagion with certain types of hpv was supposed to have a causal relationship with cervical neoplasia of the uterus. the rate of prevalence of hpv was investigated in pre-cancer and cancer patients with uterine cervical smear using virapap or viratype. according to the cytologic classification, among those whose cytology was diagnosed as class i or ii, were found a few positive hpv, however, in cases in classes cytology iiia, iii and iiib, the positive rate turned out to be 22.5, 41 and 72.4%, ... | 1991 | 1647429 |
| [detection of hpv dna in the uterine cervical lesions by polymerase chain reaction and in situ hybridization]. | human papillomavirus (hpv) is found in close association with carcinogenesis of the uterine cervix. we applied a new in vitro gene amplification technology, the polymerase chain reaction (pcr) to detect hpv 16 and 18 in cervical exfoliated cells. hpv infections were detected in 5 (16%) of 31 women with no pathological lesions of the uterine cervix (normal), 16 (24%) of 67 with cervical intraepithelial neoplasia (cin) and 6 (38%) of 16 with invasive cervical cancer. moreover, 10% formalin-fixed a ... | 1991 | 1647430 |
| detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction. | in a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/29) of biopsies were positive for human papillomavirus (hpv) type 16 dna by polymerase chain reaction. we used two oligonucleotide primer pairs (position 215-514 and 606-805) flanking a 300 and a 199 base pair fragment from the early 6 (e6) and early 7 (e7) genes. the results were concordant both with the e6 and with the e7 regions. of the amplified p ... | 1991 | 1647488 |
| perinatal vertical transmission of human papillomavirus and subsequent development of respiratory tract papillomatosis. | this study prospectively examined the potential for human papillomavirus (hpv) to be transmitted vertically to newborns during delivery. exfoliated cervical cells were extracted from 72 pregnant women during the third trimester and again during labor prior to delivery, and tested for the presence of hpv dna. these results were compared with hpv dna specimens from their newborns, who were sampled by exfoliated cells from the oral-pharyngeal cavity and vulva or tissue from the foreskin 24 to 72 ho ... | 1991 | 1647745 |
| constitutive release of il6 by human papillomavirus type 16 (hpv16)-harboring keratinocytes: a mechanism augmenting the nk-cell-mediated lysis of hpv-bearing neoplastic cells. | in the present study we demonstrate that the cultured human keratinocyte cell line (sk-v cells) harboring and expressing integrated human papillomavirus type 16 (hpv16) dna sequences constitutively releases il6, which is known as a pleiotropic immunoregulatory cytokine of potential antitumor properties. the presence of il6 activity in sk-v cell-conditioned media (sk-v cm) was demonstrated by tritiated thymidine incorporation into il6-dependent b9 murine plasmacytoma cells. the effect on b9 cells ... | 1991 | 1647881 |
| human papillomavirus infection. | 1991 | 1647899 | |
| tissue effects of and host response to human papillomavirus infection. | human papillomaviruses are a heterogeneous group of dna tumor viruses associated with hyperplastic (warts, condylomata), dysplastic (cin and vin), and malignant lesions (carcinomas) of squamous epithelium. each hpv type is preferentially associated with specific clinical lesions and has an anatomic site preference for either cutaneous or mucosal squamous epithelium. infection appears to begin in the basal cells. early gene expression is associated with acanthosis, and late gene expression is ass ... | 1991 | 1647900 |
| epidemiology of human papillomavirus infections. | human papillomavirus infection represents the most common mucocutaneous viral infection, and 3% to 5% of all patients have clinically evident warts. human papillomavirus infections of the genital tract are one of the most common sexually transmitted viral infections in the united states. data from std clinics and private physicians' offices reveal that genital warts, one manifestation of genital hpv infection, have been diagnosed more frequently in recent years. with the use of a variety of diag ... | 1991 | 1647901 |
| clinical manifestations of human papillomavirus infection in nongenital sites. | our knowledge of warts dates thousands of years. most warts represent no more than a transient infection in the hands and feet of children and adults. with the relatively recent medical advances permitting the prolonged survival of immunocompromised hosts, however, hpv-induced lesions have become an important problem. in these patients, lesions represent a recurring, intractable infection that predisposes the patient to the development of skin cancer. such problems have been appreciated for some ... | 1991 | 1647902 |
| clinical manifestations and natural history of genital human papillomavirus infection. | over the past decade, the ever-increasing volume of evidence implicating hpv types in genital neoplasia has stimulated much research interest into all aspects of the biology of this interesting group of viruses. this has led to the identification of an ever-increasing heterogeneity of hpv types. it is not surprising, therefore, that the clinical profile of disease associated with genital hpv types is much broader than previously recognized. knowledge of this clinical spectrum is mandatory to the ... | 1991 | 1647903 |
| relation of papillomaviruses to anogenital cancer. | a variety of hpv types infect the anogenital mucosa, giving rise to lesions that differ in clinical appearance, histology, and risk of malignant progression. human papillomavirus type 16 is distinguished by a strong association with high-grade intraepithelial neoplasia and the greatest prevalence in anogenital malignancy. most cancers appear to have a multifactorial cause, and hpv infection alone is probably insufficient for malignant transformation. the consistent association between hpv infect ... | 1991 | 1647905 |
| ancillary diagnostic tests for detection of human papillomavirus infection. | a variety of ancillary tests useful in the diagnosis of hpv infection are currently at the clinician's disposal. use of 5% acetic acid for detection of inapparent hpv infection is a simple procedure that can be performed in any office setting. colposcopy is useful alone or in combination with the use of acetic acid but may not be as readily available. use of laboratory-based tests is gaining popularity as an adjunctive measure, particularly in combination with papanicolaou smears, for the detect ... | 1991 | 1647906 |
| human papillomavirus infection and neoplasia. speculations for the future. | human papillomavirus infections of the skin and anogenital tract are common. the association of these viruses with neoplasia, particularly of the anogenital tract, has stimulated efforts to devise practical methods of detection and typing of hpv. although experimental diagnostic tests are available, they are, for the most part, complex and time consuming and are limited to medical centers researching hpv. new methods for preparing probes with higher sensitivities for hybridization tests will all ... | 1991 | 1647910 |
| human papilloma virus infection in dysplastic cervical smears based on the presence of koilocytes: a study of 280 cases. | in order to estimate the frequency of human papilloma virus (hpv) infection among patients and women attending the family planning clinics and having dysplastic cervical epithelium, a retrospective examination of cytology slides was undertaken at kenyatta national hospital's (knh) cytology laboratory. all slides with a diagnosis of slight dysplasia or greater were re-evaluated by the author. only slides received between 1st january 1983 and 31st december 1987 were included. observation of koiloc ... | 1991 | 1647943 |
| human papillomavirus type 16 dna in anal cancers from six different countries. | an association between anal squamous cell carcinoma and human papillomavirus (hpv) type 16 dna has been documented in the uk. if hpv type 16 is an important aetiological factor in the development of this tumour it would be expected to occur in anal cancer tissues from other parts of the world. in this study a series of 173 anal squamous cell carcinoma tissue samples from five centres around the world have been examined by dna hybridisation for hpv type 16 dna sequences. hpv type 16 dna was found ... | 1991 | 1648029 |
| detection and typing of multiple genital human papillomaviruses by dna amplification with consensus primers. | many types of human papillomavirus (hpv) are associated with genital lesions. in order to develop simple and sensitive diagnostic procedures for hpv infection, we took advantage of the polymerase chain reaction (pcr). we compared the published nucleotide sequences of the l1 region from six genital hpv types and designed a pair of consensus primers for l1 region. the pcr with the consensus primers for l1 region (l1-pcr) could amplify at least nine genital hpv types, 6, 11, 16, 18, 31, 33, 42, 52 ... | 1991 | 1648051 |
| relatively low prevalence of human papillomavirus 16, 18 and 33 dna in the normal cervices of japanese women shown by polymerase chain reaction. | ninety-two cervical scrapes and tissues, obtained from cytologically or histologically normal cervices of japanese women, were examined for the presence of human papillomavirus (hpv) 16, 18 and 33 dna by the polymerase chain reaction (pcr) method. five out of 92 cases were hpv 16 dna-positive, but neither hpv 18 nor 33 dna was detected. the hpv (type 16, 18 and 33) prevalence rate in pregnant women, including postpartum, was 10% (3/31), which was higher than that in non-pregnant women. in two hp ... | 1991 | 1648052 |
| a spectrum of bilateral squamous conjunctival tumors associated with human papillomavirus type 16. | three patients with bilateral tumors presenting as multiple keratinizing and verrucous lesions of the bulbar and tarsal conjunctiva were determined by dna amplification and hybridization studies to harbor human papillomavirus type 16 (hpv-16). results of biopsy in two patients showed infiltrating squamous cell carcinoma in one eye and dysplasia or carcinoma in situ in the fellow eye. in the third patient, focal, inflamed, hypertrophic, papillary lesions with pseudoglandular invaginations of the ... | 1991 | 1648188 |
| human papillomavirus dna types in squamous cell carcinomas of the head and neck. | previous studies have found variable evidence suggestive of a role for human papillomavirus (hpv) in squamous cell carcinoma of the head and neck. in this study 49 cases of primary verrucous or squamous cell carcinoma from patients referred to a regional medical center were examined initially by southern blot hybridization to detect hpv types 2, 6, 11, 13, 16, 18, and 32. approximately 60% of carcinomas from certain head and neck sites, particularly the floor of the mouth, tongue, pharynx, pirif ... | 1991 | 1648190 |
| detection of human papillomavirus dna by in situ dna hybridization and polymerase chain reaction in premalignant and malignant oral lesions. | the sensitivity of detection of human papillomavirus (hpv) dna in premalignant and malignant oral lesions by in situ hybridization (ish) and polymerase chain reaction (pcr) were compared. with both methods hpv dna was found in 4 of 24 cases of epithelial dysplasia, 4 of 14 cases of verrucous hyperplasia, and 1 of 10 cases of squamous cell carcinoma. the 10 cases of smokeless tobacco keratoses and 3 cases of verrucous carcinoma that we examined were all negative for hpv dna. the pcr for the e6 op ... | 1991 | 1648191 |
| in situ hybridization analysis of human papillomavirus dna in oral mucosal lesions. | commercial biotinylated dna probes specific for human papillomavirus (hpv) types 6 and 11; 16 and 18; and 31, 33, and 35 were used for in situ hybridization analysis of 105 oral mucosal specimens from 5 cases of verruca vulgaris, 15 cases of condyloma acuminatum, 30 cases of squamous papilloma, 20 cases of hyperkeratosis/acanthosis, 15 cases of epithelial dysplasia, 5 cases of carcinoma in situ, and 15 cases of squamous cell carcinoma. positive hybridization signals were found in 26 specimens (2 ... | 1991 | 1648192 |
| detection of human papillomavirus in head and neck tumors with dna hybridization and immunohistochemical analysis. | the presence of human papillomavirus (hpv) dna in oral, sinus, pharynx, and larynx lesions of japanese patients was studied by southern blot hybridization under less stringent (25% formamide, 42 degrees c) and stringent (50% formamide, 42 degrees c) conditions. three samples from 10 benign tumors, and 3 of 30 malignant tumors, contained hpv dna or hpv-related sequences. the hpv dnas harbored in three laryngeal papillomas were hpv-11, -6, and -6 or -11, respectively. the hpv dna and viral capsid ... | 1991 | 1648193 |
| in situ dna hybridization analysis of oral papillomas, leukoplakias, and carcinomas for human papillomavirus. | twenty-one papillomas, 23 ordinary benign keratoses, 13 smokeless tobacco keratoses, 10 verrucous hyperplasias, 10 verrucous carcinomas, 17 squamous cell carcinomas, 3 epithelial dysplasias, and 6 lichen planus lesions were evaluated for human papillomavirus (hpv) types 6/11, 16/18, and 31/33/35, with biotinylated double-stranded dna probes by in situ hybridization. sixty-two percent (13/21) of oral squamous papillomas were positive for hpv dna. hpv dna types 6 and 11 demonstrated the strongest ... | 1991 | 1648194 |
| the state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. | human cervical carcinoma cell lines that were either positive or negative for human papillomavirus (hpv) dna sequences were analyzed for evidence of mutation of the p53 and retinoblastoma genes. each of five hpv-positive cervical cancer cell lines expressed normal prb and low levels of wild-type p53 proteins, which are presumed to be altered in function as a consequence of association with hpv e7 and e6 oncoproteins, respectively. in contrast, mutations were identified in the p53 and rb genes ex ... | 1991 | 1648218 |
| differing characteristics of human papillomavirus rna-positive and rna-negative anal carcinomas. | anal carcinomas were tested for the presence of human papillomavirus (hpv) rna transcripts by in situ hybridization using 125i-labeled rna probes. the hpv transcripts were detected in 73.2% of 41 nonglandular anal carcinomas but in none of six anal or 11 rectal adenocarcinomas. in anal nonglandular carcinomas, no difference in the percentage of tumors that were hpv rna positive was observed between tumors classed as basaloid carcinomas or squamous cell carcinomas. patients with hpv rna-negative ... | 1991 | 1648434 |
| densitometric determination of human papillomavirus dna quantities by chromato-scanning in the fluorescence mode. | the quantitation of human papillomavirus dna isolated from warts by chromato-scanning (fluorescence mode) photographs of ethidium bromide-stained agarose gels is described. excitation at 200 nm (with a cutoff filter at 400 nm) generates fluorescence from the white portion of the printing paper. the fluorescent intensity correlated with the quantities of dna in the band of interest. the amounts of dna were determined using calibration curves of approximately the same size as lambda phage dna frag ... | 1991 | 1648568 |
| reduction of vector contamination in detection of human papillomavirus dna using full-length genomic dna probes. | nucleic acid hybridization techniques are currently the most specific and sensitive procedures available for diagnosing and typing human papillomavirus (hpv) infection. hpv genomic dna cloned into vector pbr322 or related vectors are commonly used as probes for detection of hpv. when isolating hpv insert dna however, it is difficult to remove all pbr322 dna. this can result in false positive readings when clinical specimens harbouring sequences homologous to pbr322 are screened. it was found tha ... | 1991 | 1648572 |
| human papillomavirus (hpv) dna in focal epithelial hyperplasia by in situ hybridization. | eighteen cases of focal epithelial hyperplasia (feh) were investigated for the presence of human papillomavirus (hpv) group specific antigen by immunocytochemistry and hpv types 1, 6, 11, 13, 16, 18 and 32 by dna in situ hybridization employing biotinylated probes. seven (39%) specimens demonstrated the presence of hpv group specific antigen. fifteen (83%) specimens were positive for hpv dna: 9 (60%) showed hpv 32, of which 6 were on non-keratinized mucosa and 3 on border of keratinized and non- ... | 1991 | 1648613 |
| human papillomavirus type 31 dna detected in part of the dysplasia but in no part of the squamous metaplasia in a specimen taken from one patient. | using in situ hybridization, human papillomavirus (hpv 6, 16, 18, 31, 33) dnas were detected in a cervical severe dysplasia accompanied by squamous metaplasia. it was found that, only hpv 31 dna was harbored in the cervical severe dysplasia, but hpv dnas were not identified in a lesion of squamous metaplasia. the in situ hybridization method will be of use, therefore, when dysplasia with squamous metaplasia or other lesions are examined for hpv dna. in a cervical smear, hpv 31 dna could be detec ... | 1991 | 1648636 |
| chronic ulcerations following topical therapy with 5-fluorouracil for vaginal human papillomavirus-associated lesions. | applied topically to the vagina, 5-fluorouracil (5-fu) cream is an effective therapy for human papillomavirus (hpv)-associated lesions of the vagina including condylomata acuminata and vaginal intraepithelial neoplasia. although the acute side effects of 5-fu therapy are well recognized, long-term sequelae of intravaginal 5-fu use have not been described in detail in the literature. to assess the incidence and clinical course of 5-fu-related vaginal mucosal alterations, we studied 220 patients w ... | 1991 | 1648696 |
| human papillomavirus and carcinoma of the urinary bladder. | the human papillomaviruses have been strongly associated with anogenital cancers. sporadic reports have linked papillomavirus infection to bladder neoplasms. we analyzed 27 normal and malignant bladder tissue samples for the presence of human papillomavirus by in situ dna hybridization and the polymerase chain reaction. only one invasive, keratinizing squamous cell carcinoma was found to contain human papillomavirus dna. this occurred in a 61-yr-old female who was immunocompetent and had no prev ... | 1991 | 1648724 |
| detection of genital papillomavirus types by polymerase chain reaction using common primers. | we describe the detection of eight genital human papillomavirus (hpv) types, including hpv16 and hpv18, by pcr amplification of a 323 base-pair region of the genome within the l1 open reading frame (orf). the primer sequences are: tgyaaatatccwgattwtwt and gtatcwacmacagtaacaaa. the method will detect purified hpv16 dna down to a concentration of as little as a single molecule in 100 microliters. the method is also applicable to purified dna and crude lysates from tumour biopsies. typing of the pc ... | 1991 | 1648934 |
| endoscopic removal of esophageal condylomata acuminatum containing human papilloma virus. | 1991 | 1649063 | |
| clinical and histologic features of vulvar carcinomas analyzed for human papillomavirus status: evidence that squamous cell carcinoma of the vulva has more than one etiology. | the association between the human papillomavirus (hpv) and malignant neoplasms of the uterine cervix is well established; however, its role in the pathogenesis of vulvar cancer has not been well defined. this study correlates the clinical and histopathologic features of 21 invasive carcinomas of the vulva with the presence of hpv dna as detected by southern blot and polymerase chain reaction (pcr) analysis. by one or both techniques, hpv dna was detected in 10 of the 21 tumors analyzed; all hpvs ... | 1991 | 1649118 |
| detection of human papillomavirus dna by pcr in semen from patients with and without penile warts. | to determine the prevalence of urethral hpv infection, as indicated by the presence of hpv dna in semen, in males with and without penile warts. | 1991 | 1649121 |
| occurrence of antibodies to l1, l2, e4 and e7 gene products of human papillomavirus types 6b, 16 and 18 among cervical cancer patients and controls. | sera from 118 women of 33 to over 90 years of age, with or without a history of cervical squamous-cell carcinoma, were examined for the presence of antibodies to hpv-6b, hpv-16 and hpv-18, l1, l2, e4, and e7 gene products by the use of bacterially derived beta-gal fusion proteins and western-blot analysis. among the cervical cancer patients, 29/46 (63.0%) were positive for antibodies to e4 and/or e7 of hpv-16 and/or e7 of hpv-18. in contrast, only 2 of 31 (6.5%) non-genital cancer patients and 4 ... | 1991 | 1649138 |
| on a possible protective effect of hla-a11 against skin cancer and keratotic skin lesions in renal transplant recipients. | renal transplant recipients who have skin cancer potentially related to human papillomavirus were hla typed with a special focus on hla-a11, which in nonimmunosuppressed patients is negatively associated with the occurrence of virus-related carcinoma of the cervix. we found also a negative association between hla-a11 and skin cancer; none of the 66 transplant recipients with skin cancer were positive for hla-a11. as hla-a11 seems to have a protective effect against skin cancer, we speculate that ... | 1991 | 1649229 |
| atypical squamous cells. a case-series study of the association between papanicolaou smear results and human papillomavirus dna genotype. | thirty women with "atypical" squamous cells but not cervical intraepithelial neoplasia (cin) on their papanicolaou smears were tested for the presence of human papillomavirus (hpv) with the southern blot hybridization technique. the papanicolaou smears were reviewed for the presence of atypical squamous cells according to patten's criteria. comparison groups consisted of 30 patients with normal, 29 patients with cin i and 24 patients with cin ii papanicolaou smears. ten of the 30 women (33%) wit ... | 1991 | 1649307 |
| determinants of genital human papillomavirus infection in young women. | carcinoma of the cervix has several well-established epidemiologic risk factors, including multiple sexual partners and early age at first intercourse. human papillomavirus (hpv) infection appears to have an etiologic role in the development of cervical neoplasia, but evidence linking hpv infection to known risk factors for cervical cancer has been inconsistent. the lack of expected correlations may be due to the inaccuracy of hpv assays previously used. a polymerase chain reaction dna amplifica ... | 1991 | 1649312 |
| biologic properties and nucleotide sequence analysis of human papillomavirus type 51. | human papillomaviruses (hpvs) may be grouped according to the site from which they are isolated and the disease with which they are associated. we recently identified and cloned hpv type 51 (hpv-51) from a low-grade precancerous lesion (g. nuovo, e. devilliers, r. levine, s. silverstein, and c. crum. j. virol. 62:1452-1455, 1988). molecular epidemiologic analysis of cervical lesions, including condylomata and low- and high-grade precancers, revealed that hpv-51 was present in about 5% of the sam ... | 1991 | 1649326 |