Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| expression of high-affinity laminin receptor mrna correlates with cell proliferation rather than invasion in human papillomavirus-associated cervical neoplasms. | induction of the expression of the mr 67,000 high-affinity laminin receptor gene has been postulated as playing a role in the progression of human tumors to invasive cancers. we tested this hypothesis by examining histopathological sections of a large number of epithelial lesions of the genital tract associated with human papillomaviruses. in situ hybridization was performed with a riboprobe generated from a laminin receptor complementary dna. laminin receptor mrna was expressed primarily in the ... | 1992 | 1311633 |
| prevalence of human papillomavirus in young women. | 1992 | 1311775 | |
| penile verrucous carcinoma: a clinicopathologic, human papillomavirus typing and flow cytometric analysis. | the relationship of various verruciform squamous cell proliferations of the penis such as verrucous carcinoma, with or without anaplasia and giant condyloma, is uncertain. we conducted clinicopathologic, flow cytometric, and hpv typing studies on 15 cases of penile verrucous carcinoma to investigate its place in the spectrum of genital squamous proliferations. the results show a high degree of morphologic uniformity with respect to ackerman's original diagnostic criteria, as well as to several o ... | 1992 | 1311839 |
| human papillomavirus dna detection in papanicolaou-stained cervical smears with a nonradioactive, in situ hybridization assay. | archived papanicolaou-stained cervical smears from women with different cervical pathologies were processed for human papillomavirus (hpv) dna detection and typing with an in situ hybridization (ish) assay that employed commercial biotinylated hpv dna probes. two hpv dna probes were utilized: one included hpv genotypes 6/11 and the other, 16/18. the method yielded positive results for hpv dna 6/11 in 5 cases with condylomata acuminata (100%) and in 2 of 47 with flat warty lesions (4.2%), whereas ... | 1992 | 1311885 |
| early-stage squamous cell and adenocarcinoma of the cervix. | articles on early-stage squamous cell and adenocarcinoma of the cervix published between august 1990 and july 1991 are reviewed. a new monoclonal antibody used to distinguish endocervical from endometrial differentiation is described, as well as a histochemical means of distinguishing in situ from invasive adenocarcinoma. in vitro and in vivo studies of cell lines immortalized with human papillomavirus dna are described with a discussion of the mechanism of the development of malignancy. an anim ... | 1992 | 1311965 |
| clinical applications of molecular biologic screening for human papillomavirus: diagnostic techniques. | 1992 | 1311996 | |
| prevalence of human papillomavirus types 16 and 18 in squamous-cell carcinoma of the penis: a retrospective analysis of primary and metastatic lesions by differential polymerase chain reaction. | human papillomaviruses (hpv), particularly types 16 and 18, may be carcinogenic effectors in a variety of human lower-genital-tract malignancies. using the highly sensitive technique of differential polymerase chain reaction (d-pcr) with amplimers from the e6 open reading frames of hpv types 16 and 18, a retrospective analysis of a 20-year institutional experience with squamous-cell carcinoma of the penis (sccp) was performed to determine the prevalence of these hpv types in this malignancy. par ... | 1992 | 1312062 |
| intermolecular triplex formation distorts the dna duplex in the regulatory region of human papillomavirus type-11. | a conformational distortion in the dna duplex at the regulatory region of human papillomavirus type-11 next to an intermolecular triplex, formed with a synthetic oligonucleotide, was investigated with several chemical probes. the sequence targeted for triplex formation borders on the binding sites for the regulatory proteins encoded by the viral e2 open reading frame. dimethyl sulfate, diethyl pyrocarbonate, and oso4 all react to a greater extent with nucleotides in the duplex that are immediate ... | 1992 | 1312091 |
| seroreactivity against hpv 16 e4 and e7 proteins in renal transplant recipients and pregnant women. | 1992 | 1312108 | |
| cervical cancer--what role for human papillomavirus? | objective: to review the role of human papillomavirus (hpv) as a causative agent of cancer of the cervix. data sources, data synthesis, study selection: medical journals, recently published text books related to cancer of the cervix and hpv and papillomavirus reports were examined to review the pathology of cervical cancer and its precursor lesions, its epidemiology in australia and overseas, methods of detection of hpv (in particular molecular biology techniques used to diagnose hpv) and eviden ... | 1992 | 1312213 |
| the 'other' sexually transmitted diseases. chlamydial, herpes simplex virus, and human papillomavirus infections. | the incidence of sexually transmitted diseases continues to rise across the country. because many cases are subclinical and asymptomatic, chlamydial, herpes simplex virus, and human papillomavirus (hpv) infections continue to spread at an alarming rate. these infections can lead to serious sequelae, such as pelvic inflammatory disease, infertility, and cervical dysplasia or carcinoma, so improved disease-control strategies are needed. preventive efforts should include use of the latest diagnosti ... | 1992 | 1312236 |
| antibodies to hpv-16 e6 and e7 proteins as markers for hpv-16-associated invasive cervical cancer. | transforming proteins e6 and e7 of human papillomaviruses (hpvs) are consistently expressed in hpv-associated cervical cancers. in elisa with four hpv-16 e6-e7 peptides, patients with hpv-16-associated invasive cervical cancer (group 1) had a greater seroreactivity than all other groups, which included patients with hpv-16-associated cervical intraepithelial neoplasia, invasive cervical cancer patients without hpvs, and unaffected controls. a larger proportion of group 1 sera, as compared to ser ... | 1992 | 1312268 |
| differing prevalence of human papillomavirus rna in penile dysplasias and carcinomas may reflect differing etiologies. | penile intraepithelial neoplasias grade 3 (pin 3) and penile carcinomas were examined for the presence of human papillomavirus (hpv) rna transcripts by in situ hybridization using 125i-labeled rna probes. human papillomavirus transcripts were detected in all 10 pin 3 lesions not associated with invasive malignant conditions but were present in only 29% of penile carcinomas (9 of 26 squamous cell carcinomas and none of 5 verrucous carcinomas). human papillomavirus rna-positive penile cancers were ... | 1992 | 1312299 |
| laboratory techniques in the investigation of human papillomavirus infection. | 1992 | 1312507 | |
| human antibodies recognize multiple distinct type-specific and cross-reactive regions of the minor capsid proteins of human papillomavirus types 6 and 11. | human serum samples derived from a case-control study of patients with cervical carcinoma (n = 174) or condyloma acuminatum (n = 25) were tested for the presence of immunoglobulin g antibodies to human papillomavirus type 6 (hpv6) l2 and hpv11 l2 recombinant proteins in a western immunoblot assay. thirty-six samples (18%) were positive for hpv6 l2 antibodies alone, 25 (13%) were positive for hpv11 l2 antibodies alone, and 34 (17%) were positive for both hpv6 l2 and hpv11 l2 antibodies. thirty sa ... | 1992 | 1312618 |
| molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind. | we have amplified by the polymerase chain reaction, cloned, and sequenced genomic segments of 118 human papillomavirus type 16 (hpv-16) isolates from 76 cervical biopsy, 14 cervical smear, 3 vulval biopsy, 2 penile biopsy, 2 anal biopsy, and 1 vaginal biopsy sample and two cell lines. the specimens were taken from patients in four countries--singapore, brazil, tanzania, and germany. the sequence of a 364-bp fragment of the long control region of the virus revealed 38 variants, most of which diff ... | 1992 | 1312620 |
| the e6 and e7 genes of human papillomavirus type 6 have weak immortalizing activity in human epithelial cells. | previous studies have shown that the e7 gene of human papillomavirus (hpv) type 16 or 18 alone was sufficient for immortalization of human foreskin epithelial cells (hfe) and that the efficiency was increased in cooperation with the respective e6 gene, whereas the hpv6 e6 or e7 gene was not active in hfe. to detect weak immortalizing activities of the hpv6 genes, cells were infected with recombinant retroviruses containing hpv genes, alone and in homologous and heterologous combinations. the hpv ... | 1992 | 1312623 |
| structure-function analysis of the human papillomavirus type 16 e7 oncoprotein. | the e7 gene of human papillomavirus type 16 encodes a multifunctional nuclear phosphoprotein that is functionally and structurally similar to the adenovirus (ad) e1a proteins and the t antigens of other papovaviruses. e7 can cooperate with an activated ras oncogene to transform primary rodent cells, trans activate the ad e2 promoter, and abrogate transforming growth factor beta-mediated repression of c-myc. recent studies suggest that these functions may in part be a consequence of the ability o ... | 1992 | 1312637 |
| high-risk human papillomavirus types in cytologically normal cervical scrapes from kenya. | seventy-seven women with normal cervical cytology on routine visit to a family planning clinic in nairobi, kenya, were analysed for genital human papillomavirus (hpv) types by polymerase chain reaction (pcr). we applied a general primer pair (gp60/gp124) recognising sequences conserved among hpv types 6, 11, 16, 18, 31 and 33. of the 77 specimens tested 15 (19.5%) proved to be positive for genital hpv. amplification products were examined for the presence of high-risk hpv types by slot-blot hybr ... | 1992 | 1312661 |
| lack of immortalizing activity of a human papillomavirus type 16 variant dna with a mutation in the e2 gene isolated from normal human cervical keratinocytes. | the oncogenic potential of a human papillomavirus type 16 (hpv16) variant cloned from normal human cervical keratinocytes has been tested in vitro using primary rodent epithelial cells and human cervical keratinocytes. the hpv16 variant was able to extend the lifespan of, but failed to immortalize, human keratinocytes. it could however cooperate with an activated ras oncogene to transform primary rodent cells. radioimmunoprecipitation assays of the rodent cells showed that they expressed the e7 ... | 1992 | 1312701 |
| a comparison of simultaneous cervical cytology, hpv testing, and colposcopy. | this pilot study explores the screening techniques for premalignant and malignant disease of the cervix. given current knowledge of the etiology and progression of cancer of the cervix, should family physicians screen patients with papanicolaou (pap) smears, human papillomavirus (hpv) smears, colposcopy, or some combination of these three? in a retrospective audit of 75 patients comparing simultaneous pap smears and colposcopy, 5 of 8 patients with biopsy-proven cervical intraepithelial neoplasi ... | 1992 | 1312758 |
| colposcopy in family practice: pilot studies of pain prophylaxis and patient volume. | in a family practice office with volume representative of an average one-person practice, the implementation of colposcopy services was studied. during six months, 257 patients received papanicolaou (pap) smears. these patients represented 16.2% of the total 1,585 female patient visits for ages greater than 16. of the 257 cytology specimens, 10.1% (26) revealed dysplasia or evidence of human papilloma virus (hpv), indicating the need for colposcopic examination. pathology reports of all biopsied ... | 1992 | 1312759 |
| oral folic acid supplementation for cervical dysplasia: a clinical intervention trial. | we attempted to evaluate the effect of oral folic acid supplementation on the course of cervical dysplasia. | 1992 | 1312773 |
| genital human papillomavirus infections in adolescents: implications for evaluation and management. | genital infections caused by human papillomaviruses (hpv) have become the most prevalent sexually transmitted disease in the united states. rates of infection with these agents are particularly high among adolescents. the most common clinical expression of genital hpv infection is condyloma acuminata, a condition that dermatologists are frequently called on to treat. the identification of external genital warts in a teenager raises questions that impact directly on the patient's evaluation and m ... | 1992 | 1312856 |
| a simple and rapid method of high quantity dna isolation from cervical scrapes for detection of human papillomavirus infection. | infection with human papillomavirus (hpv) is an important etiological factor in the development of cervical cancer, and detection of the viral genome is of prognostic importance, particularly for preneoplastic lesions. we developed a simple, easy and efficient non-organic method of dna extraction from cervical scrapes for reliable detection of hpv dna sequences. the method involves incubation of cell nuclei in higher concentration of proteinase k at 65 degrees c for 2.5 h. following prolonged in ... | 1992 | 1313040 |
| ribavirin mitigates wart growth in rabbits at early stages of infection with cottontail rabbit papillomavirus. | the challenge to develop antiviral agents effective against dna viruses such as human papillomavirus (hpv) has been dependent on finding an animal model which mimics the human forms of the disease. we have used an existing model system for the purpose of measuring the effect of antiviral drugs on the inhibition of growth of these lesions. this was based upon domestic rabbits which efficiently grow cutaneous papillomas (warts) when infected with cottontail rabbit papillomavirus (crpv). one agent ... | 1992 | 1313222 |
| hiv-1 infectivity of human carcinoma cell lines lacking cd4 receptors. | human immunodeficiency virus type 1 (hiv-1) prototype, hiv1 lav, and a zairian virus hiv1 ndk, an isolate highly cytopathic for cd4+ lymphocytes, were used to infect eleven different cd4 negative non-lymphoid human cell lines. eight of the lines were derived from carcinomas wherein human papillomavirus was thought to have been etiologic. all these cell lines lacked cd4 receptor and cd4 specific mrna. after cocultivation with sensitive cem cells, hiv-1 lav was rescued from six infected cell lines ... | 1992 | 1313332 |
| synchronous squamous and glandular neoplasia of the anal canal. | a 48 year old man presented with invasive adenocarcinoma in the wall of a non-healing anal fistula. the subsequent abdomino-perineal resection specimen showed residual invasive carcinoma coexisting with in situ carcinoma of anal glands as well as in situ squamous carcinoma of the anal canal. the epithelium of the anal canal had koilocytotic features. dna hybridisation studies by the dot blot technique showed weak positivity for human papillomavirus (hpv) subtypes 16, 18. this case illustrates a ... | 1992 | 1313454 |
| absence of human papillomavirus in cervical adenocarcinoma. | 1992 | 1313458 | |
| children with condylomata acuminata. | the modes of transmission of genital human papillomavirus (hpv) infection in children are controversial. studies have varied in reporting suspicion of sexual abuse in children with condylomata acuminata from zero to 90.9%. possible modes of transmission include sexual, from mother to infant in utero, passage through an infected birth canal, infection of a nongenital type virus to the genital area, and nonsexual acquisition from a fomite. | 1992 | 1313489 |
| disruption of either the e1 or the e2 regulatory gene of human papillomavirus type 16 increases viral immortalization capacity. | the "high-risk" human papillomavirus types 16 (hpv-16) and 18 (hpv-18) have been etiologically implicated in the majority of human cervical carcinomas. in these cancers, the viral dnas are often integrated into the host genome so that expression of the e1 and the e2 genes is lost, suggesting that disruption of these regulatory genes plays an important role in carcinogenic progression. previous studies defining the viral genes affecting hpv-16 transformation functions have used the "prototype" vi ... | 1992 | 1313584 |
| [male genital examination: the usefulness of penis endoscopy and the acetic acid test for the detection of papillomavirus lesions]. | this study was based on 2,400 genital examinations performed by means of the acetic acid test looking for human papillomavirus (hpv) lesions in the male partners of women with hpv genital lesions. these peniscopies demonstrated hpv lesions in 56% of the men examined. in 109 cases, histological and virological examination revealed that 30% of them had areas of intraepithelial neoplasia of the penis, associated with potentially oncogenic papillomavirus infection. the value of this examination is t ... | 1992 | 1313666 |
| induction of cytotoxic t lymphocytes specific for a syngeneic tumor expressing the e6 oncoprotein of human papillomavirus type 16. | human papillomavirus (hpv) type 16 has been implicated in the etiology of cervical carcinomas, but it is unknown whether hpv-specific immunity can function in controlling the growth of hpv-associated carcinomas. we previously demonstrated that cd8+ t lymphocytes can inhibit the in vivo outgrowth of murine tumor cells transfected with the hpv-16 e7 gene and have now established a murine model to study the ctl responses to the e6 oncoprotein of hpv-16. immunization of c3h/hen mice with syngeneic f ... | 1992 | 1313847 |
| spontaneous production of growth factors for human lymphocytes from a human papillomavirus type 18-contained foreskin fibroblast cell line. | an immortalized fibroblast cell line, designated as ccfs-1/kmc, derived from human neonatal foreskin fibroblasts, contained human papillomavirus (hpv) type 18 dna. since this newly established cell line could spontaneously secrete activating factors for normal human blood lymphocytes, the synthesis and release of potent inflammatory cytokines from this cell line were checked. to determine the presence of cytokines in the supernatant collected from the cell line, tests by a cytokine-specific elis ... | 1992 | 1313887 |
| nucleotide 880 splice donor site required for efficient transformation and rna accumulation by human papillomavirus type 16 e7 gene. | mutations within coding sequences of the various human papillomavirus type 16 (hpv-16) genes have been used to demonstrate that the hpv-16 e7 gene is necessary and sufficient for transformation of rodent cells. we now provide evidence that, in addition to e7 coding sequences, a small cis-acting region immediately flanking the 3' end of e7 coding sequences is also required for transformation. this was shown by translation termination linker insertion, progressive deletion analysis, and site-direc ... | 1992 | 1313897 |
| human papillomavirus type 33 in a tonsillar carcinoma generates its putative e7 mrna via two e6* transcript species which are terminated at different early region poly(a) sites. | human papillomavirus type 33 (hpv-33)-specific early region transcripts in a tonsillar carcinoma were analyzed by using the rna polymerase chain reaction method. a total of five cdna species including species with potential to encode e6*i, e6*ii, and e6*iii, could be identified. as determined by 3' cdna end mapping, one e6*i cdna species was found to utilize a novel early region poly(a) site and was polyadenylated at or near the putative initiation codon of the e1 open reading frame (orf). compa ... | 1992 | 1313922 |
| replication of plasmid-derived human papillomavirus type 11 dna in cultured keratinocytes. | plasmid-derived human papillomavirus type 11 (hpv11) dna has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes. hpv11 dna was excised from the bacterial plasmid, religated to form circular molecules, and cotransfected along with psv2neo. transfectants were selected and shown to contain replicated episomal hpv dna. once selected, hpv11 dna persists in cells through at least two additional passages. | 1992 | 1313926 |
| human immunodeficiency virus, human papillomavirus, and cervical intraepithelial neoplasia in nairobi prostitutes. | to evaluate the impact of human immunodeficiency virus (hiv) on human papillomavirus (hpv) and cervical intraepithelial neoplasia (cin), a study was conducted of 147 hiv-seropositive and 51 hiv-seronegative prostitutes in nairobi, kenya. among the women infected with hiv, 10 (7%) had signs or symptoms of significant hiv-related disease, and the remaining 93% were asymptomatic. the prevalence of cervical hpv dna was 37% among hiv-seropositive women and 24% in hiv-seronegative women (odds ratio [o ... | 1992 | 1313992 |
| the prevalence of human papillomavirus in cervical lesions with koilocytosis and/or cin i. | thirty-one patients with koilocytosis and/or concomitant cin i were analysed for the presence of hpv types 11, 16 and 18 by in situ hybridization and southern blot analysis. the prevalence of hpv was 48% and 55%, respectively, when measured by the two methods and among the hpv positive, hpv 11 and 16 were present in 47% and 60%, respectively, whereas hpv 18 was not found. | 1992 | 1314062 |
| genetic alterations by human papillomaviruses in oncogenesis. | the integration sites in the cellular genome of human papillomavirus are located in chromosomal regions always associated with oncogenes or other known tumor phenotypes. two regions, 8q24 and 12q13, are common to several cases of cervical carcinoma and can have integrated more than one type of papillomavirus dna. these two chromosomal regions contain several genes implicated in oncogenesis. these observations strongly imply that viral integration sites of dna tumor viruses can be used as the acc ... | 1992 | 1314194 |
| prevalence, diagnosis and treatment of lower genital neoplasia in women with human immunodeficiency virus infection. | the prevalence of lower genital neoplasia and human papilloma-virus-related genital lesions were evaluated in a cohort of 75 women with human immunodeficiency virus type 1 (hiv-1) infection at different stages of hiv disease. the overall rate of cervical intraepithelial neoplasia (cin) in the group studied was 29.3% (22/75). eight out of 10 high-grade cin lesions contained 'high-risk' hpv-dna 16/18 and/or 31/35/51 as demonstrated by 'in situ' hybridization with biotinylated probes. vulvar and/or ... | 1992 | 1314201 |
| [the search for, detection and control of the human papillomavirus (hpv)]. | the research, detection and control of the human virus of papilloma has called the investigators attention because of the verification of its direct participation as a cofactor in the origin of premalign and malign lesions in the genital organs. this is the objective of the present bibliographic reviewing in which we are trying to move gynecologists to establish a management protocol in the external consultation, so that it will become possible to decrease the highest index of female morbimortal ... | 1992 | 1314211 |
| oncogenesis of squamous carcinoma of the uterine cervix. | carcinoma of the uterine cervix is the most frequent neoplasm among women in india, accounting for up to 85% of all female gynecological malignancies. in the united states, it accounts for about 48% of all female tumors and 4% of all cancer deaths of females. epidemiological evidence suggests involvement of numerous risk factors in the etiology of cervical cancer, including sexual behavior, number of pregnancies, cigarette smoking, and venereal disease. recent studies, however, tend to emphasize ... | 1992 | 1314235 |
| human papillomavirus prevalence in pregnancy. | conventional wisdom has long held that human papillomavirus (hpv) prevalence is increased in pregnancy. we examined cervical swabs of 375 women for hpv dna using polymerase chain reaction (pcr) and virapap with an expanded probe range. of this population, 115 were pregnant (42 in the first trimester, 46 in the second, and 27 in the third) and 100 were postpartum. the control population consisted of 160 women who were otherwise similar to the pregnant population but were not pregnant or postpartu ... | 1992 | 1314358 |
| natural history of cervical human papillomavirus lesions does not substantiate the biologic relevance of the bethesda system. | the newly introduced bethesda system proposes to replace the terms dysplasia, carcinoma in situ (cis), or cervical intraepithelial neoplasia (cin) with two terms, low-grade squamous intraepithelial lesion (sil) or high-grade sil. we tested the biologic relevance of the bethesda system (ie, its ability to establish a close correlation between biologic behavior and the different grades of lesions) in a series of 528 women with genital human papillomavirus (hpv)-associated precancerous lesions pros ... | 1992 | 1314359 |
| repeated evaluation of human papillomavirus 16 status in cervical swabs of young women with a history of normal papanicolaou smears. | this study was designed to compare single-point prevalence estimates with a cumulative prevalence estimate of human papillomavirus (hpv) type 16 in cervical smears. the influence of the menstrual cycle and the long-term effect of hpv 16 positivity on the development of cervical intraepithelial neoplasia (cin) were monitored. we examined 21 women (median age 23.6 years) every 5 weeks for 1 year. all women had a history of negative papanicolaou smears for at least 5 years before enrollment. cervic ... | 1992 | 1314360 |
| recombinant retroviruses encoding human papillomavirus type 18 e6 and e7 genes stimulate proliferation and delay differentiation of human keratinocytes early after infection. | human papillomavirus (hpv) dnas are detected in most genital dysplasias and cancers, suggesting that these viruses perturb epithelial growth and differentiation. the e6 and e7 genes of hpv type 18 induce immortality in keratinocytes cultured from genital tract epithelia, and the immortal cell lines display aberrant squamous differentiation. to examine whether the e6 and e7 proteins directly alter keratinocyte growth and differentiation, high-titer recombinant retroviruses were constructed for ef ... | 1992 | 1314365 |
| in situ hybridization with digoxigenin-labelled dna probes for the detection of human papillomavirus-induced focal epithelial hyperplasia among venezuelans. | an in situ hybridization assay with digoxigenin-labelled probes was used to detect the presence of human papillomavirus (hpv) sequences in ten related venezuelan patients with the diagnosis of focal epithelial hyperplasia. the samples displayed hpv sequences in all cases. further restriction analysis in four of the patients suggested the presence of hpv-13 in oral lesions. | 1992 | 1314447 |
| glucocorticoid-dependent transformation by human papillomavirus type 16 e7 coding and 3' noncoding sequences. | the establishment of transformation of primary rodent cells by human papillomavirus (hpv) type 16 dna requires glucocorticoid hormones (pater et al., nature 335, 832-835, 1988). here we provide evidence by mutational analysis that, in the context of the hormone-regulated hpv 16 promoter/enhancer, the only protein coding sequences of hpv 16 required are those of the e7 gene. moreover, additional sequences adjacent to the 3' end of e7 coding sequences are also essential for the establishment of th ... | 1992 | 1314461 |
| unregulated and basal transcriptional activities of the regulatory sequence of the type 18 human papillomavirus genome in transgenic mice. | type 18 human papillomavirus (hpv18) is a genital virus closely associated with cervical carcinoma. to analyze the transcriptional activities of the long control region (lcr) of the hpv18 genome, we have produced 12 transgenic mice harboring the hpv18/lcr sequence fused to a promoterless sv40 t-antigen (tag) gene. the mice were small in body size, generally very weak, and none lived longer than 110 days. three mice with the longest life span (58-110 days) developed hyperplastic thymus and/or lym ... | 1992 | 1314463 |
| antibody response to human papillomavirus (hpv) type 11 in children with juvenile-onset recurrent respiratory papillomatosis (rrp). | we previously established, using an elisa, the presence of specific antibodies directed at human papillomavirus (hpv) type 11 virions in the sera of patients with condylomata acuminata, mostly a disease of young adults that, like recurrent respiratory papillomatosis (rrp), is caused by two closely related hpvs, types 6 and 11. the present study was done to investigate if children with rrp can make viral-specific antibodies to an infection that is acquired at birth. using the same elisa, we studi ... | 1992 | 1314464 |
| prognostic importance of human papillomavirus type 16 dna in cervical cancer. | human papillomavirus (hpv) type 16 dna is frequent in invasive cervical cancers. among 43 patients with invasive cervical cancer, hpv-16-positive tumors spread to the parametrial and pelvic lymph nodes significantly more often than did hpv-16-negative tumors (p less than 0.05). demonstration of hpv-16 dna in invasive cervical cancers may be an additional prognostic factor for this disease. | 1992 | 1314692 |
| differential cooperation of a carcinogen with human papillomavirus type 6 and 16 dnas in in vitro oncogenic transformation. | in contrast to the strong association of human papillomavirus (hpv) type 16 with genital malignancies, hpv 6 has been found essentially in benign genital lesions. in these studies we show that hpv type 6 and 16 dnas behave differently also in their ability to transform nih 3t3 cells in cooperation with the carcinogen n-methyl-n'-nitro-n-nitrosoguanidine (mnng). although we could show that both hpv-6- and hpv-16-transfected genomes were integrated and expressed in nih 3t3 cells, only the nih 3t3 ... | 1992 | 1314784 |
| assessment by planimetry of langerhans' cell density in penile epithelium with human papillomavirus infection: changes observed after topical treatment. | langerhans' cells could have an important role in the immune surveillance for the malignant transformation associated with certain viral infections. a planimetric study of langerhans' cells was performed on epithelial sheets obtained from male and female patients who presented with human papillomavirus-induced condylomata. preliminary epidemiological studies have indicated that after a human papillomavirus infection the risk of malignant transformation is higher in the cervix than in the penis. ... | 1992 | 1314911 |
| human papillomavirus type 16 e7 protein inhibits dna binding by the retinoblastoma gene product. | the human papillomavirus e7 gene can transform murine fibroblasts and cooperate with other viral oncogenes in transforming primary cell cultures. one biochemical property associated with the e7 protein is binding to the retinoblastoma tumor suppressor gene product (prb). biochemical properties associated with prb include binding to viral transforming proteins (e1a, large t, and e7), binding to cellular proteins (e2f and myc), and binding to dna. the mechanism by which e7 stimulates cell growth i ... | 1992 | 1314947 |
| [human papillomavirus, cervical intraepithelial cancer and immunosuppression. two cases in renal transplant recipients]. | immunosuppression is therapeutically induced in an increasing number of patients, exceeding largely the only transplant recipients. its carcinological complications are a major preoccupation, among which the uterine cervix intraepithelial neoplasia. the strong relationship between these genital neoplasia and the lesions caused by human papillomavirus is now evident and has to be taken in consideration for an early and regular follow-up of these women. cervical smear tests, colposcopic assessment ... | 1992 | 1315074 |
| detection of human papillomavirus infection in diseased and nondiseased sites of the respiratory tract in recurrent respiratory papillomatosis patients by dna hybridization. | we examined 12 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their disease, for the presence of human papillomavirus (hpv). biopsies were obtained from their respiratory papillomas and nondiseased sites (nds) of the respiratory tract: the nasopharynx, posterior tonsillar pillar, aryepiglottic fold, cervical trachea, intrathoracic trachea, and bronchi. the presence of hpv dna was determined by using the virapap/viratype dna hybridization procedure. ... | 1992 | 1315130 |
| comparison of a one-step and a two-step polymerase chain reaction with degenerate general primers in a population-based study of human papillomavirus infection in young swedish women. | the prevalence of human papillomavirus (hpv) infection in cervical cell scrapes from young women was determined by polymerase chain reaction (pcr) by using general primer pairs localized within the l1 region. with a one-step general pcr, 5.9% (35 of 590) of young women in a population-based study were found to contain hpv dna. the proportion of hpv-positive women increased with age, from 1.4% (1 of 69) among women aged 19 years to 9.2% (13 of 142) among women aged 25 years. among the cervical sc ... | 1992 | 1315341 |
| risk level, knowledge, and preventive behavior for human papillomaviruses among sexually active college women. | during the past decade, there has been mounting scientific evidence linking human papillomavirus (hpv) with cervical cancer and, at the same time, a great increase in physician consultations for hpv infections. hpv infection risk factors include multiple sex partners, early age at first intercourse, history of sexually transmitted diseases (stds), and smoking. this study surveyed 263 sexually active college women and concluded that (1) they are at considerable risk for contracting hpv, (2) they ... | 1992 | 1315349 |
| detection of hpv dna in archival specimens of cervical cancer using in situ hybridisation and the polymerase chain reaction. | an archival survey of 98 cervical cancer specimens dating from the 1920s to the 1980s was undertaken to determine whether changes had occurred in the prevalence of human papilloma-virus (hpv) dna. hpv dna was detected in paraffin sections of cancers fixed in 10% formalin by in situ hybridisation (ish) using hpv 6, 11, 16, and 18 32p-labelled dna probes under conditions of high stringency; and by the polymerase chain reaction (pcr) using 20-mer oligonucleotide primers to amplify 109 bases of the ... | 1992 | 1315371 |
| human papillomavirus type 16 dna in oral white sponge nevus. | white sponge nevus (wsn) is a benign hereditary lesion of the mucous membranes. dna extracted from a biopsy specimen of oral wsn was assayed for the presence of dna sequences homologous to human papillomavirus (hpv) types 1, 2, 4, 6, 11, 13, 16, and 18 by southern blot hybridization. only hpv-16 homologous dna sequences were detected at a copy number of approximately 200 to 250 genome copies per diploid cell. the viral dna sequences did not appear to be integrated into the host cell chromosome. ... | 1992 | 1315429 |
| comparison of southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix. | to compare the efficiency of hybridization methods for the detection of hpv genome, 22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by southern blot analysis and in situ hybridization carried out with 35s- and biotin-labeled probes. these cases contained from less than one to as many as 50 copies per cell of hpv 16 and 18 types. to increase the sensitivity of biotinylated probes, a silver enhancement procedure of the peroxidase reaction product was applied. resul ... | 1992 | 1315439 |
| detection of human papillomavirus type 6f genome in nasal papillomatosis. | five cases of nasal papillomatosis were studied clinicopathologically and virologically. in a case of recurrent papillomatosis of non-inverted type located on the nasal septum, human papillomavirus (hpv) dna was detected by dot blot hybridization with an rna cocktail probe of mucosal hpvs. in southern blot hybridization, the dna hybridized with that of hpv types 6 and 11 but not with those of types 16 and 18. its restriction endonuclease-cleavage patterns corresponded well to those of hpv type 6 ... | 1992 | 1315475 |
| human papillomavirus infection of the uterine cervix. tissue sampling and laboratory methods affect correlations between infection rates and dysplasia. | two common tissue sampling techniques--colposcopic biopsy and cervical scrape--and two common human papillomavirus (hpv) detection techniques--southern blot and dot blot (sb and virapap [vp])--were compared to determine whether differences in these techniques alter correlations between "oncogenic" hpvs and cervical neoplasia. in 87 women with persistently abnormal papanicolaou (pap) smears, concurrent biopsy and scrape specimens contained hpv in 21 (24%) and contained no hpv in 26 (30%); 30 scra ... | 1992 | 1315486 |
| detection of human papillomavirus in matched cervical smears and biopsy specimens by non-isotopic in situ hybridisation. | to determine the relative diagnostic sensitivity of non-isotopic in situ hybridisation (nish) for the diagnosis of human papillomavirus (hpv) on matched smears and biopsy specimens; to compare the nish signal type in the two samples; and to correlate the nish data with the morphological diagnosis. | 1992 | 1315807 |
| a high frequency of human papillomavirus dna sequences in cervical carcinomas of indian women as revealed by southern blot hybridization and polymerase chain reaction. | ninety-six colposcopically directed biopsies from squamous epithelial carcinoma of the uterine cervix and 22 age-matched normal control biopsy specimens were examined by both southern blot hybridization and polymerase chain reaction (pcr) for the presence of different human papillomavirus (hpv) dna types. cancer of the uterine cervix, which is the most common malignant disease in indian women, showed a high frequency (98%) of hpv as compared to those reported from other parts of the world. hpv t ... | 1992 | 1315836 |
| cervicovaginal human papillomavirus infection in adolescents and young adults. | 1992 | 1315857 | |
| polymerase chain reaction and direct dna tests in detection of human papillomavirus (hpv) dna in cytologically normal and abnormal cervical smears. scandinavian multicenter study group. | the detectability of human papillomavirus dna from cervical smears was studied by dna tests of varying sensitivity. the results were correlated with cervical cytologic abnormalities. a total of 326 women examined at the gynecological outpatient clinics of five scandinavian hospitals were enrolled in the study on the basis of previous pathological cervical smear or clinically suspected human papillomavirus (hpv) infection. the cervical smears were examined for hpv-dna of types 6/11, 16 or 18 by t ... | 1992 | 1316053 |
| a morphologic, pathologic, and virologic study of anogenital warts in men. | infection with human papillomavirus (hpv) in the anogenital region is associated with benign papillomas (condyloma acuminatum), subtle verrucous changes, subclinical infection, and malignant lesions. although both men and women are affected, much of the investigation has been directed toward women in the study of cervical and vulvar carcinoma. the current investigation focuses on hpv infection in men. this study was undertaken to correlate the clinical spectrum of disease in our population of ma ... | 1992 | 1316102 |
| keratoacanthomas: human papillomavirus associated? | 1992 | 1316106 | |
| anal verrucous carcinoma associated with human papilloma virus type 11: magnetic resonance imaging and flow cytometry evaluation. | 1992 | 1316107 | |
| detection of human papillomavirus (hpv) using dot blot and southern blot, hybridizing with a mixture of seven probes. | a previously presented method for the detection and typing of human papillomavirus (hpv) dna has been modified for the simultaneous analysis of hpv types 6, 11, 16, 18, 31, 33 and 35. the method has two steps, where a dot blot test is used to exclude cases that do not hybridize with hpv-dna. the remaining cases are then analysed by a southern blot procedure, using a mixture of subgenomic probes for the simple and accurate analysis of hpv types. when the procedure was used for the analysis of cli ... | 1992 | 1316129 |
| herpes simplex virus and human papillomavirus sites correlate with chromosomal breakpoints in human cervical carcinoma. | the distribution of 1,912 breakpoints observed in a series of 148 cervical cancers was analyzed. fifty bands were shown to be nonrandomly involved in chromosome structural rearrangements. one hundred thirty-three breaks were noted in bands known to contain a human papillomavirus integration site, and 454 breaks were noted in bands containing a herpes simplex virus breakage site. we suggest that herpes simplex viruses and, possibly, papillomaviruses play an important role in the carcinogenesis an ... | 1992 | 1316222 |
| chromosome fragility in lymphocytes of women with cervical uterine lesions produced by human papillomavirus. | we studied 30 women with cervical lesions that showed human papillomavirus infection (hpv). cervical hpv infection was diagnosed by cytology, histology, immunohistochemistry, and electron microscopy, as well as by dna viral hybridization in situ with 6, 11, 16, and 18 hpv types. three groups of patients were studied: 15 women infected by hpv of 6 and 11 types with koilocytic lesions and benign evolution, 15 women infected by hpv of 16 and 18 types with koilocytic lesions and malignant evolution, ... | 1992 | 1316223 |
| a placebo controlled observer blind immunocytochemical and histologic study of epithelium adjacent to anogenital warts in patients treated with systemic interferon alpha in combination with cryotherapy or cryotherapy alone. | to examine biopsy specimens of tissue immediately adjacent to anogenital (ag) warts which had been treated with either cryotherapy plus subcutaneous interferon (ifn) alpha 2a or cryotherapy alone, for histological features of (a) human papilloma virus (hpv) infection (b) localised cellular immune responses, to further characterise any cellular immune infiltrates with tissue immunocytochemistry, and to relate any histological, immunocytochemical findings to the treatment response of nearby ag war ... | 1992 | 1316307 |
| sexually transmitted diseases in children: human papillomavirus infection. | 1992 | 1316309 | |
| progression of squamous cell carcinoma of the uterine cervix from cervical intraepithelial neoplasia infected with human papillomavirus: a retrospective follow-up study by in situ hybridization and polymerase chain reaction. | a retrospective study was conducted to reveal the natural history of cervical carcinoma infected with human papillomavirus (hpv) and to document latent persistence of hpv infection. thirty-eight formalin-fixed, paraffin-embedded hysterectomy specimens of cervical carcinoma and cervical intraepithelial neoplasia (cin) iii were examined for the presence of hpv 6, 11, 16, 18, 31, 33, and 35 dna by in situ hybridization (ish) with biotinylated dna probes. the hpv 6 and 11 were not detected, but hpv ... | 1992 | 1316320 |
| effect of human papillomavirus infection on estrogen receptor and heat shock protein hsp27 phenotype in human cervix and vagina. | in this study we have explored whether, as a consequence of human papillomavirus (hpv) infection, there is inappropriate expression of estrogen receptor and/or of a small heat shock protein of 27,000 daltons (hsp27). estrogen receptor, hsp27, and hpv structural antigens were detected by immunocytochemistry, while hpv dna (6/11, 16/18, 31/35/51) was determined by in situ hybridization in cervical and vaginal samples from 40 patients. most of the samples with hpv infection without atypia showed a ... | 1992 | 1316321 |
| detection with the polymerase chain reaction of human papillomavirus dna in condylomata acuminata treated in vitro with liquid nitrogen, trichloroacetic acid, and podophyllin. | the mechanisms of action for local treatments used against condylomata acuminata are unknown, but most are believed to cause physical destruction of infected tissue. | 1992 | 1316388 |
| tampons: a novel patient-administered method for the assessment of genital human papillomavirus infection. | assessment of human papillomavirus (hpv) infection usually requires a speculum examination to collect genital specimens. a technique using tampons as a patient-administered method for the collection of specimens was studied by dot blot hybridization (hpv types 6, 11, 16, 18, 31, and 33) and polymerase chain reaction (pcr). tampons and cervical scrapes were collected from 48 consecutive women attending a dysplasia clinic. tampons provided a significantly larger pellet volume (p less than .002) an ... | 1992 | 1316411 |
| increased detection of hpv 16 virus in invasive, but not in early cervical cancers. | human papilloma virus type 16 (hpv 16) dna is found in about 50% of cervical squamous cell carcinomas (sccs), and this association has raised the possibility of a causal role for hpv 16 in cervical carcinogenesis. we have tested this hypothesis by assaying a series of biopsies (n = 119) ranging from normal mucosa to infiltrating scc with the pcr-technique for the presence of hpv 16 dna. while hpv 16 dna was detected in 50% of our cases with invasive scc, the incidence of hpv 16-positive samples ... | 1992 | 1316424 |
| late promoter of human papillomavirus type 8 and its regulation. | human papillomavirus type 8 (hpv8) belongs to the hpv types associated with skin carcinomas of patients with epidermodysplasia verruciformis (ev). its noncoding regulatory sequences (ncr) were shown to drive the expression of the reporter gene chloramphenicol acetyltransferase (cat) in transient assays with human epithelial cells (ht3 cells). this constitutive activity could be enhanced by coexpression of the hpv8 transactivator protein e2. the analysis of 5' deletions of the ncr showed that the ... | 1992 | 1316464 |
| two ap1 sites binding junb are essential for human papillomavirus type 18 transcription in keratinocytes. | the activity and epithelial tropism of the human papillomavirus type 18 p105 early promoter, which directs the synthesis of the e6 and e7 transforming genes, are controlled by cis elements included in the viral long control region. to identify potential cellular regulators of this promoter, we mutagenized one or both of the 5'-tgactaa-3' cis elements capable of interacting with the ap1 transcription factor, which is composed either of homodimers or heterodimers of the jun products or of heterodi ... | 1992 | 1316480 |
| efficiency of binding the retinoblastoma protein correlates with the transforming capacity of the e7 oncoproteins of the human papillomaviruses. | the human papillomaviruses (hpvs) associated with genital tract lesions can be classified as either "high risk" or "low risk" based on their association with human anogenital cancer. the e7 proteins of the high-risk and the low-risk viruses are quite similar in their amino acid composition and structural organization yet differ in their transforming potential and in a number of biochemical properties. a series of chimeric proteins consisting of segments of the high-risk hpv-16 and the low-risk h ... | 1992 | 1316608 |
| adenovirus e1a, simian virus 40 tumor antigen, and human papillomavirus e7 protein share the capacity to disrupt the interaction between transcription factor e2f and the retinoblastoma gene product. | the adenovirus e1a gene product, the simian virus 40 large tumor antigen, and the human papillomavirus e7 protein share a short amino acid sequence that constitutes a domain required for the transforming activity of these proteins. these sequences are also required for these proteins to bind to the retinoblastoma gene product (prb). recent experiments have shown that e1a can dissociate complexes containing the transcription factor e2f bound to prb, dependent on this conserved sequence element. w ... | 1992 | 1316611 |
| presence of human papillomavirus 6/11 dna in condyloma acuminatum of the urinary bladder. | a japanese woman with condyloma acuminatum of the urinary bladder is presented. the condyloma acuminatum lesion was resected endoscopically and human papillomavirus 6/11 dna was detected. after treatment, there has been no recurrence of the disease. | 1992 | 1316664 |
| papilloma virus infection. precancer and epidermoid cancer. | we present a review of the relationship between human papillomavirus (hpv), precancer and epidermoid cancer of the uterine cervix, co-factors that contribute in cervical carcinogenesis and the mechanism through which papillomaviruses transform normal cells into neoplastic cells by inducing an overproduction of the encoded proteins. we also offer final remarks and conclusions on this pathology that increases its incidence every year. | 1992 | 1316842 |
| detection of human serum antibodies that neutralize infectious human papillomavirus type 11 virions. | a selection of human sera were tested for the presence of antibodies that neutralized infectious human papillomavirus (hpv) type 11. neutralizing antibodies were detected by prevention of hpv-11-induced condylomatous transformation of human foreskin chips transplanted subrenally into athymic mice. test sera were obtained from 21 female patients with genital condylomas and eight patients with laryngeal papillomas. control patients consisted of 57 adult random blood donors and five asymptomatic ch ... | 1992 | 1316943 |
| t cell responses to the human papillomavirus type 16 e7 protein in mice of different haplotypes. | the response of murine t cells to the e7 molecule of human papillomavirus type 16 (hpv-16) was studied using eight different mouse strains of six distinct h-2 haplotypes. hpv-16 e7 protein was prepared as a fusion protein with glutathione-s-transferase, purified by affinity chromatography and used for immunization. cells from the lymph nodes were cultured with whole fusion protein, glutathione-s-transferase or hpv-16 e7 protein synthetic peptides. all the mouse strains tested, with the exception ... | 1992 | 1316944 |
| mutagenesis of human papillomavirus types 6 and 16 e7 open reading frames alters the electrophoretic mobility of the expressed proteins. | the e7 open reading frames of human papillomavirus type 6 (hpv-6) and hpv-16 encode proteins consisting of 98 amino acids that are quite similar in sequence yet different in electrophoretic mobility. moreover, these proteins vary strikingly in oncogenicity. to investigate the molecular basis of the differences in structure and function, site-directed mutagenesis was used to exchange non-conserved amino acid residues between the two proteins. the mutated coding regions were expressed as fusion pr ... | 1992 | 1316945 |
| c-myc and c-ha-ras cellular oncogenes and human papillomaviruses in benign and malignant cutaneous lesions. | to analyze the role of human papillomavirus (hpv) infection and c-myc and c-ha-ras oncogene activation in cutaneous and mucosal lesions, serial frozen sections of 47 lesions from grafted recipients and 10 biopsies from non-immunosuppressed patients were examined. hela, caski, mcf7, colo 320 and 3t3 cells, which contain various copy numbers of hpv dna and/or c-myc gene, were used as controls. hpv, myc and ras oncogene dnas were not detected in normal epithelia by in situ hybridization with biotin ... | 1992 | 1317208 |
| characterization of an immortalized human cell line derived from neonatal foreskin diploid fibroblasts. | a new human skin cell line, designated as ccfs-1/kmc, immortalized from human neonatal foreskin diploid fibroblast cells, has been subcultured successfully in vitro for more than 500 passages. this anchorage-dependent cell line possesses many common features of transformation such as morphological and cytoskeletal changes, hypotriploidy, infinite lifespan, increasing plating efficiency and saturation density, and decreasing serum requirement and population doubling time. human papillomavirus (hp ... | 1992 | 1317393 |
| human papillomavirus (hpv) types specific of epidermodysplasia verruciformis detected in warts induced by hpv3 or hpv3-related types in immunosuppressed patients. | epidermodysplasia verruciformis (ev) is characterized by an abnormal genetic predisposition to infection with specific types of human papillomavirus (hpv). specific defects of the cell-mediated immunity and/or of the control of hpv infection in keratinocytes are assumed to be involved in the development of the disease. as a model to test this hypothesis, we have studied the prevalence of ev-specific hpv in skin warts of 56 immunosuppressed patients. all main types of cutaneous hpv (hpv1, 2, 3, 4 ... | 1992 | 1317396 |
| what's new in diagnosis and treatment of hpv-associated cervical lesions. | the human papilloma virus (hpv) has been subject to intense discussion during the last decade, as evidence has accumulated to strongly suggest that viruses play an essential part in the genesis of cervical neoplasms. the contamination rate of the female population is reported to range between 5 and 80%, depending on the modality of detection. many indications almost reliably suggest the oncogenicity of certain hpv-types. the highest progression rate was found in hpv 16 lesions, followed by hpv 1 ... | 1992 | 1317558 |
| human papillomavirus dna in cervix. in-situ hybridization with biotinylated probes on bouin's fixed paraffin embedded specimens. | we examined retrospectively a series of 65 bouin's fixed, paraffin-embedded tissue specimens from 8 condylomatous lesions, 16 condylomas associated with cervical intraepithelial neoplasia (cin), and 12 neoplasia without condylomatous signs, for histological characteristics, the detection of viral structural antigen, the presence and typing of hpv dna by molecular in situ hybridization with biotinylated probes types 6, 11, 16 and 18 under stringent conditions (tm - 12 degrees c). hpv dna was pres ... | 1992 | 1317560 |
| 'hit and run' oncogenesis by human papillomavirus type 18 dna. | transfection of an immortalized cell line (ae), derived from syrian hamster embryo cells, with human papillomavirus type 18 (hpv 18) dna induced morphological transformation and these transformed cells were tumorigenic in nude mice. southern blot analysis revealed that the transfected viral dna was retained in all the cell lines tested, however, all these transformed cells contained only less than one copy per cell of viral genome. eleven cloned cell lines were established from a tumor cell line ... | 1992 | 1317646 |
| evaluation of sexually abused and nonabused young girls for intravaginal human papillomavirus infection. | the objective of this study was to compare the prevalence of intravaginal human papillomavirus-associated disease in two groups of girls to develop information regarding the means of transmission of anal-genital human papillomavirus disease. | 1992 | 1317670 |
| papillomavirus found in anorectal squamous carcinoma, not in colon adenocarcinoma. | we used polymerase chain reaction dna amplification methods for the detection and typing of genital human papillomaviruses in paraffin-embedded tissue sections of five patients with anorectal squamous cell carcinoma and 22 patients with colonic adenocarcinoma. the cases were further tested by in situ hybridization with biotin-labeled probes specific for human papillomavirus types 6/11, 16/18, and 31/33/35. by polymerase chain reaction, human papillomavirus dna was demonstrated in all of the case ... | 1992 | 1317698 |
| an elisa capture assay for the e7 transforming proteins of hpv16 and hpv18. | elisa capture assays were established for the e7 transforming proteins of hpv16 and hpv18, based on a range of previously characterised polyclonal and monoclonal antibodies. no cross-reactivity was observed in the elisas between hpv18 e7 and hpv16 e7. immunoreactive e7 protein (ie7) was measured in a series of hpv-transformed cell lines, and ranged from 0.6 to 17.7 ng ie7/mg cell protein. ie7 was labile at 22 degrees c (t1/2 = 37 min) but relatively more stable at 4 degrees c (t1/2 = 210 min). h ... | 1992 | 1317876 |
| integration of human papillomavirus types 16 and 18 in cervical adenocarcinoma. | to determine which type of human papillomavirus (hpv) is associated with cervical adenocarcinoma and whether the virus was integrated or episomal in two continents. | 1992 | 1317883 |