Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bowenoid papulosis. | bowenoid papulosis usually appears as small papules on the penis or vulva. histologically, these lesions display the atypia of bowen's disease (squamous cell carcinoma in situ). bowenoid papulosis is now considered to be a sexually transmitted disease, probably caused by human papillomavirus type 16. malignant transformation has been documented. female sexual partners of men with this disease may be at increased risk of cervical cancer. | 1988 | 2839970 |
| detection of human papillomavirus deoxyribonucleic acid from vulvar dystrophies and vulvar intraepithelial neoplastic lesions. | vulvar biopsy specimens from 16 patients with gross lesions of the vulva consistent with vulvar dystrophy were examined histologically and for the presence of human papillomavirus deoxyribonucleic acid by southern blot hybridization. none of the nonatypical dystrophies probed positive for human papillomavirus; however, three of five atypical hyperplastic dystrophies (vulvar intraepithelial neoplasia) contained human papillomavirus deoxyribonucleic acid type 16. we conclude that human papillomavi ... | 1988 | 2839984 |
| [papillomavirus and cancer]. | papillomaviruses are causative agents of benign tumor (papilloma) and are widely distributed in most of animals. the papillomaviruses are small dna viruses grouped in the papovavirus family. the genomes of all papillomaviruses pce double stranded circular dna of approximately 8 k. base pairs. classification of the viruses is presently based on the host range and relatedness of the nucleic acids. in the case of human papillomavirus (hpv), more than 50 types have been isolated. the amount of infor ... | 1988 | 2840032 |
| subclinical human papillomavirus infections in male sexual partners of female carriers. | the male sexual partners of 156 women with human papillomavirus infection of the cervix uteri were examined. in 120 men (77 per cent) penile lesions were found on examination of the penis via a colposcope (peniscopy) the most common of which were flat acetowhite lesions (53 per cent). predilection sites of lesions were the urethral meatus and the corona glandis (23 and 19 per cent of the lesions, respectively). using filter in situ hybridization human papillomavirus-deoxyribonucleic acid was fou ... | 1988 | 2848137 |
| human papillomavirus type 16 cooperates with activated ras and fos oncogenes in the hormone-dependent transformation of primary mouse cells. | the effect of human papillomavirus (hpv) and activated oncogenes on the growth and morphology of primary baby mouse kidney (bmk) cells has been studied. early region dna from hpv types 16, 18, 31, and 33, but not type 6, under the transcriptional control of a heterologous, retroviral promoter cooperated with ej-ras to produce cell lines that gave rise to carcinomas in syngeneic immunocompetent animals. the same hpv constructs, when cotransfected with a plasmid containing sequences from the finke ... | 1988 | 2848240 |
| prevalence of human papillomavirus type 18 dna in adenocarcinoma and adenosquamous carcinoma of the uterine cervix occurring in japan. | to identify whether the incidence of human papillomavirus (hpv) type 18 dna in adenocarcinoma and adenosquamous carcinoma is attributable to the histological types or geographical differences, the presence of hpv-16 and hpv-18 dna in carcinoma of the uterine cervix from japan were studied by in situ hybridization using tritium labeled hpv dna probes. hpv-18 dna was detected in 5 of 11 cases (45%) of adenocarcinoma, one case of adenocarcinoma in situ and 2 of 3 cases of adenosquamous carcinoma. i ... | 1988 | 2848332 |
| the responsiveness of human papillomavirus upstream regulatory regions to herpes simplex virus immediate early proteins. | we have tested the responsiveness of the upstream regulatory regions (urr) of human papillomaviruses (hpv) to transactivation by the herpes simplex virus type 1 (hsv-1) immediate early proteins icp4 (vmw175) and icp0 (vmw110), using recombinant plasmids which contain urrs of hpv 1, 11 or 16 upstream of an sv40-promoted cat gene. transfection of the hpv-cat plasmids into mouse cells expressing a temperature-sensitive icp4 protein enabled us to demonstrate that icp4 enhances transcription of the h ... | 1988 | 2848381 |
| correlation of histologic types of carcinoma of the uterine cervix and human papillomavirus and herpes simplex virus type 2 dna sequences in the uterine cervical biopsies. | forty patients with invasive cancer of cervix from new delhi were examined for the presence of human papillomavirus (hpv) and herpes simplex virus (hsv) type 2 dna sequences in a southern hybridization assay. data were analysed according to the histologic type of cancer. hpv dna sequences were detected in 82.5% biopsies and hsv-2 dna sequences were found in 10% biopsies. hpv-16 dna sequences were found either alone or together with hpv-18 and/or hsv-2 bgl ii n fragment in 55% biopsies from kerat ... | 1988 | 2848476 |
| human papillomavirus & herpes simplex virus type 2 dna sequences with chronic cervicitis & invasive cervical cancer. | 1988 | 2848763 | |
| mapping of two novel transcripts of bovine papillomavirus type 4. | a cdna library was synthesized using rna from bovine papillomavirus type 4 (bpv-4)-induced papillomas. the major viral transcript was characterized by sequencing of its cdna, primer extension mapping and s1 nuclease protection studies. the transcript initiates at multiple sites between nucleotide (nt) 777 and nt 902, contains a splice junction between nt 1016 and nt 3376 and terminates at nt 4034, using the early polyadenylation signal at nt 4009. sequencing of viral cdnas and mrna revealed devi ... | 1988 | 2848926 |
| penile/anal condylomas and squamous cell cancer. a hpv dna hybridization study. | acuminate condylomas from the penis (n = 17) and anus (six cases), three anal/penile giant condylomas, anal bowen's disease (four cases), and intraanal squamous cell carcinomas with associated condylomatous changes (10 cases) including two verrucous carcinoma were studied for human papillomavirus (hpv) infections with nick translated, biotinylated cdna probes for hpv 6, 11, 16 and 18. in addition, six cases of flat white penile lesions designated as lichen sclerosus et atrophicus were examined. ... | 1988 | 2849229 |
| oral cancer in children under 15 years of age. a clinicopathological and virological study. | 11 malignant tumours of the oral region in children under 15 years of age were diagnosed in finland during the 20-year period from 1961 to 1980; the age adjusted annual incidence rate being 0.31 per 1,000,000 children. 5 cases were intraoral cancer and 5 cases malignant tumours of the major salivary glands. the formalin-fixed tumours were studied using the in situ dna hybridization technique for adenovirus, group ii, epstein-barr and human papillomavirus, type 16. for the first time, evidence wa ... | 1988 | 2849281 |
| detection of ebv and hpv dna sequences in oral "hairy" leukoplakia by in situ hybridization. | oral "hairy" leukoplakia (ohl) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (hiv) positive acquired immunodeficiency syndrome (aids)-risk patients. the lesion has been reported to be associated with epstein-barr virus (ebv) and human papillomavirus (hpv). twenty surgical biopsy specimens were evaluated for the presence of hpv genus-specific antigen, hpv 2/4, 6/11, and 16/18 dna and ebv dna by in situ hybridization employing formal ... | 1988 | 2849634 |
| human papillomavirus (hpv) dna sequences in oral precancerous lesions and squamous cell carcinoma demonstrated by in situ hybridization. | a series of routinely processed, paraffin-embedded biopsies from 73 surgically treated oral precancerous lesions (opl) (22 cases), and oral squamous cell carcinomas (scc) (51 cases), was first screened using an in situ dna hybridization technique with a human papillomavirus (hpv) dna probe cocktail containing the 35s-labelled dna of hpv types 6, 11, 13, 16, 18 and 30. the specific hpv types in lesions shown to contain hpv dna in this procedure were further analysed by using in situ hybridization ... | 1988 | 2849647 |
| detection of human papillomavirus deoxyribonucleic acid in exfoliated cervicovaginal cells as a predictor of cervical neoplasia in a high-risk population. | specific types of human papillomavirus are currently implicated as etiologic agents of precancerous and cancerous lesions of the cervix. we have previously described the use of cervicovaginal lavage and molecular hybridization to detect human papillomavirus infections of the cervix. we report here the predictive value of this method of human papillomavirus detection to identify women with biopsy proved dysplastic and cancerous lesions of the cervix. one hundred ninety-one women from a city hospi ... | 1988 | 2849881 |
| human papillomavirus in paired normal and abnormal cervical biopsies--implications for treatment. | a total of 143 consecutive patients with abnormal cervical cytology was examined at a large colposcopy clinic in glasgow. each patient had paired biopsies from normal and abnormal cervical epithelium examined both histologically and by immunoperoxidase staining for human papillomavirus (hpv) infection. more than 71% of the abnormal biopsies and 39% of the normal paired biopsies had histological evidence of hpv infection. cytological evidence of hpv infection was seen in 38.5% of cervical smears. ... | 1988 | 2849984 |
| factors associated with progression of cervical human papillomavirus (hpv) infections into carcinoma in situ during a long-term prospective follow-up. | in the course of a prospective study of 508 women with papillomavirus (hpv) lesions of the uterine cervix, 66 lesions that progressed into carcinoma in situ (cis) were identified and treated by conization during a mean follow-up period of 35 months. the lesions were investigated with light microscopy and with in-situ dna hybridization using 35s-labelled probes for hpv 6, 11, 16, 18, 31 and 33. after radical cone treatment, 11 of the 66 women (16.7%) have presented with a recurrent hpv infection. ... | 1988 | 2849985 |
| cervical human papilloma virus infection: is treatment possible? | 1988 | 2850340 | |
| detection of human papillomavirus dna in cell scrapes and formalin-fixed, paraffin-embedded tissue of the uterine cervix by filter in situ hybridisation. | filter in situ hybridisation (fish) was used to detect the presence of dna of human papillomavirus (hpv) types 6/11 or 16/18 in cell scrapes (cytofish) and formalin-fixed, paraffin-embedded biopsies (histofish) taken from the uterine cervices of 19 women. paraffin tissue sections collected for histofish were either digested with pepsin or lysed with alkali/triton x-100. the digest or lysate of the tissue sections and cell scrapes were applied to nylon or nitrocellulose membranes for nucleic acid ... | 1988 | 2850343 |
| [screening by colposcope of partners of women with genital human papillomavirus infection]. | 1988 | 2850515 | |
| cervical human papilloma virus infection and colposcopy. | 1988 | 2850790 | |
| [co2 laser therapy and typing of human papilloma virus in follow-up studies of cervix intraepithelial neoplasms]. | in 104 woman patients with cervical intraepithelial neoplasia (cin) i-iii who had been treated between 1981 and may 1986 in the dysplasia consulting-room of the department of gynecology by means of conisation effected by laser, we checked on therapeutic success via cytology and colposcopy as well as by human papilloma virus (hpv) typing. in 90.3% of the cases 1.25 laser treatments sufficed for healing of the lesion. relapses occurred mainly in severe intraepithelial neoplasias with identified hp ... | 1988 | 2840323 |
| prospects for human papillomavirus vaccines and immunotherapies. | 1988 | 2840515 | |
| tolerance and efficacy of recombinant human interferon gamma in the treatment of refractory genital warts. | the intralesional administration of recombinant interferon alpha has led to resolution of genital warts in 50 to 70 percent of cases. however, continuous warts do not respond to such treatment, and human papillomavirus (hpv) infection of other anatomic sites remains untreated, so infection may continue to be transmitted. since interferons have shown promise as effective therapies for genital warts, we decided to investigate the tolerance and efficacy of recombinant human interferon gamma in the ... | 1988 | 2840824 |
| future trends in cervical cancer. | the identification of the close association of certain types of human papillomavirus with the development of cervical cancer should lead to an extensive revision of appropriate health policies. having taken into account the drawbacks inherent in the existing data (stemming from the use of varying nomenclature, diagnostic methods and reliability, registration and screening practices) it is possible to conclude that the incidence of hpv infections, all premalignant and malignant stages of cervical ... | 1988 | 2841018 |
| anogenital warts of the condyloma acuminatum type in hiv-positive patients. | anogenital warts of the condyloma acuminatum type seem to occur quite often during hiv infection. these warts--according to our study--are not commonly caused by malignancy-associated human papilloma virus types, but by types 6 and 11 as seen in the nonimmune-compromised population. widespread condylomata acuminata may appear in rather early stages of hiv infection and they may therefore represent early warning signs of hiv infection. | 1988 | 2841174 |
| transformation of primary human fibroblast cells with human papillomavirus type 16 dna and ej-ras. | human papillomavirus type 16 (hpv type 16) has been implicated as an etiological agent in human cervical cancer. this virus contains sequences which, under the proper transcriptional control, can increase the tumorigenicity of established mouse cells and cooperate with ej-ras in transforming primary baby rat kidney (brk) cells. these data argue that this virus contains oncogenic sequences. because this is a human virus, it was important to study the effect on primary human cells of hpv type-16 d ... | 1988 | 2841248 |
| epithelial alterations adjacent to 111 vulvar carcinomas. | material from 111 invasive primary vulvar carcinomas was reviewed in order to study the histopathologic changes adjacent to the neoplasia. the histopathologic characteristics of the adjacent tissue were divided into categories. dystrophic lesions were adjacent to invasive cancer in 57.6% of the cases, carcinoma in situ (cis) in 21.6% and epithelial changes suggestive of human papillomavirus infection in 18.9%. a spectrum of epithelial changes, ranging from hyperplastic dystrophy without atypia t ... | 1988 | 2841458 |
| intraepithelial and invasive neoplasia of the vulva in association with human papillomavirus infection. | colposcopic examination of 335 women with cytologically detected human papillomavirus (hpv) revealed involvement of the cervix in 316 patients (94%), vagina in 276 (82%) and vulva in 148 (44%). a symptom complex of pruritus and superficial dyspareunia was found in 98 of the 148 patients with vulvar infection (66%). histologic examination revealed hpv-associated vulvar intraepithelial neoplasia (vin) in 11 of 148 biopsies (7.4%). follow-up of the patients with hpv infection with or without vin sh ... | 1988 | 2841459 |
| association of human papillomavirus with vulvodynia and the vulvar vestibulitis syndrome. | seven women presenting with longstanding introital dyspareunia and burning in the vulvar area were demonstrated, with dna hybridization techniques, to harbor human papillomavirus (hpv). three of the seven had intermittent, culture-negative dysuria coincident with the vulvar complaints. all the patients had mild to marked erythema of the openings of the minor vestibular glands, with exquisite tenderness on palpation with a cotton-tipped applicator. a regular histologic examination was equivocal f ... | 1988 | 2841460 |
| alterations in the regulatory region of the human papillomavirus type 6 genome are generated during propagation in escherichia coli. | we analyzed the long control regions (lcrs) of seven human papillomavirus type 6b (hpv-6b) clones, which contained prototype hpv-6b sequences recloned into various plasmid vectors and propagated in different strains of escherichia coli. southern blot analysis and dna sequencing demonstrated three different sequences, each distinct from the published prototype hpv-6b sequence. two of the plasmids contained insertions of 24 and 94 base pairs (bp) and a 1-bp deletion. four plasmids contained insert ... | 1988 | 2841482 |
| [ultrastructural observation of human papillomavirus particles in the uterine cervix intraepithelial neoplasia]. | using transmission electron microscopy (tem), twenty two cases of an intraepithelial lesion in the uterine cervix have been examined for the presence of human papillomavirus (hpv) particles. in 21 of these cases (95%), hpv particles were detected in the nucleus, and in 5 cases, in the cytoplasm. the distribution of the intranuclear hpv particles was classified into 4 types. in only 2 cases did the exhibited particles show a geometrical crystalline array (type i). in most cases, the exhibited par ... | 1988 | 2841511 |
| search for human papillomaviruses in normal, hyperplastic, and neoplastic endometria. | twenty-eight endometria, including seven normal, five hyperplastic, and 16 neoplastic tissues, were analyzed with the southern blot hybridization technique for their possible association with human papillomaviruses (hpvs). human papillomavirus probes with types 6/11, 16, 18, and 31 failed to identify hpv dna in any of these tissues. the findings suggest that the endometrium is not a usual target for hpv infection, and that hpv is not likely to be a carcinogenic factor in the development of endom ... | 1988 | 2841629 |
| analysis of individual human papillomavirus types in cervical neoplasia: a possible role for type 18 in rapid progression. | histologic and molecular analyses of 214 cervical biopsy specimens were performed to test the hypothesis that certain individual human papillomavirus types that are usually grouped together are differentially distributed in various grades of cervical intraepithelial neoplasia and invasive squamous carcinoma. specifically, types 16 and 18, which are commonly grouped together, were analyzed separately and compared. biopsies obtained from three different geographic sites in the united states and br ... | 1988 | 2841858 |
| human papillomavirus-16 and genital cancer: are tests for the viral gene expression in vitro indicators for risk factors in vivo? | human papillomaviruses are found in up to 90% of all cervical carcinomas and are considered to play a causal role in the etiology of this malignancy. the genome of human papillomaviruses consists of a single circular dna molecule with a size of approximately 8000 basepairs. 90% of this genome encodes proteins involved in functions such as neoplastic transformation of the host cell or formation of the viral capsid. the remaining 10% of the genome, which is termed upstream regulatory region (urr), ... | 1988 | 2841899 |
| expression of human papillomavirus type 6b e2 gene product with dna-binding activity in insect (bombyx mori) cells using a baculovirus expression vector. | human papillomavirus type 6b (hpv6b) has been shown to be a major etiologic agent of genital condylomas. the e2 gene, one of the early genes, has been shown to activate the enhancer element in trans in cells transformed with bovine papillomavirus type 1a (bpv1a) but the e2 gene product of any hpv has not been identified. the e2 gene of hpv6b was inserted into the polyhedrin gene of a baculovirus, bombyx mori nuclear polyhedrosis virus (bmnpv), 156 bp downstream from the translational start codon ... | 1988 | 2842232 |
| correlation of histology with human papillomavirus dna detection in the female genital tract. | genital condyloma and intraepithelial neoplasia secondary to human papillomavirus (hpv) infection are characterized by perinuclear halos and marked nuclear atypia (koilocytotic atypia) on cytologic and histologic examination. however, at times the histologic findings, including the degree of nuclear atypia, may be suggestive but not absolutely diagnostic of an hpv related neoplasm. hpv dna sequences were detected in 63 and 56% of colposcopically visible vaginal and cervical lesions, respectively ... | 1988 | 2842237 |
| synthetic oligonucleotide probes for the detection of human papilloma viruses by in situ hybridisation. | using the published nucleotide sequence data for human papilloma virus (hpv) types 1, 6 and 16, sequences of 30 bases in length from the beginning of the e6 open reading frame (orf) were selected. oligonucleotides were synthesised on an oswel gene synthesizer and labelled at the 3' end with biotin using the enzyme terminal transferase. in situ hybridisation was carried out on paraffin sections of wart and cervical tissues mounted on silanated slides. a 2 h hybridisation step allowed the whole pr ... | 1988 | 2842356 |
| disseminated warts and evolving squamous cell carcinoma in a patient with acquired immunodeficiency syndrome. | a patient with acquired immunodeficiency syndrome developed widespread verrucous lesions on the hands, feet, and penis. histologic analysis revealed typical changes of verruca vulgaris and, from several sites, a transformation toward squamous cell carcinoma. southern blot analysis of deoxyribonucleic acid from penile wart tissue indicated the presence of a type of human papillomavirus that rarely is associated with carcinoma. | 1988 | 2842383 |
| prevalence of genital hpv infections in a regularly screened population in the netherlands in relation to cervical cytology. | to determine the prevalence of human papilloma virus (hpv) genotypes in relation to cervical cytology, 1,290 cervical samples from a regularly screened population of 30-55-year-old women were investigated. gynaecological specimens, obtained from the cervix, were cytologically classified and screened for the presence of hpvs 6/11 and 16/18 using dot-spot dna hybridisation. of the cervical samples containing unequivocally normal cells, 21 of 1,271 (1.6%) were found positive for hpv, and of the cer ... | 1988 | 2842442 |
| adenocarcinoma of the cervix associated with human papillomavirus. | eleven pure adenocarcinomas of the cervix were analyzed for human papillomavirus (hpv) types 6, 11, 16, 18 and 31 by southern blot hybridizations. seven tumors were positive for papillomavirus dna. five hybridized to the hpv 18 specific probe and two tumors hybridized to hpv 16. all tumors were negative for hpv 6/11 and 31, and no additional tumors became positive with hybridizations under nonstringent conditions to a mixture of hpv probes. no papillomavirus dna was detected in 11 noncervical ma ... | 1988 | 2843277 |
| involvement of human papillomavirus type 8 genes e6 and e7 in transformation and replication. | we investigated the transforming activity of human papillomavirus type 8 (hpv8) by expressing all early open reading frames from a heterologous promoter in different rodent fibroblast lines. morphological transformation was observed only in g418-selected mouse c127 and rat 1 cells containing an intact e6-coding region. e6 of hpv8 did not transform nih 3t3 cells as did e6 of bovine papillomavirus type 1. c127 cells transformed by e6 were anchorage independent and had a reduced serum requirement b ... | 1988 | 2843666 |
| human papillomavirus--a study of male sexual partners. | male sexual partners of a cohort of women with genital-tract abnormalities which were associated with human papillomavirus infection were examined for evidence of infection with human papillomavirus. of the 214 male partners who were examined, 93.5% had visible genital lesions. of the 196 lesions that were biopsied, 72.5% showed histological evidence of infection with human papillomavirus, and only 20.4% of subjects with histological evidence of human papillomavirus were aware of a lesion. an un ... | 1988 | 2843730 |
| high-risk factors for cervical cancer. an epidemiological study in swa/namibia. | in an epidemiological study conducted in swa/namibia to document the prevalence of high-risk factors for cervical cancer 754 women belonging to a number of ethnic groups were examined. the prevalence of cervical intra-epithelial neoplasia and human papillomavirus infection was among the highest reported in the world. there was also an alarming incidence of other sexually transmitted diseases and disturbingly low usage of contraceptive methods. there is an urgent need for a comprehensive, well-or ... | 1988 | 2843990 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| genital papillomavirus infection after treatment for cervical intraepithelial neoplasia (cin) iii. | genital human papillomavirus (hpv) infection was studied in 150 women after conization for cervical intraepithelial neoplasia grade three (cin iii). colposcopically directed biopsies were taken from the cervix and vulva for histopathological diagnosis. 77 specimens were further analyzed immunohistochemically for the presence of hpv capsid antigen. in ten randomly selected cervical biopsies cellular dna was dot blot hybridized with hpv 6/11 and 16/18 dna probes. genital warts were seen in 10 (7%) ... | 1988 | 2844389 |
| human papillomavirus dna in condylomata acuminata from japanese males. | we examined condylomata acuminata from japanese males for the presence of human papillomavirus (hpv) genomes by southern blot hybridization. hpv 6/11-related dna was found in 91% (32/35) of the condylomata. hpv 6a dna was found in 40% (14/35), hpv 6c dna in 6% (2/35), and hpv 11a dna in 37% (13/35). hpv 6-related dna, which had an unusual psti-cleavage pattern, was detected in one sample. types and subtypes of hpv dna in the samples studied (hpv 6a, 6c, and hpv 11a dna) were not correlated with ... | 1988 | 2844469 |
| managing women with human papillomavirus changes in cervical cytology. | of 86 women with cervical cytological evidence of human papillomavirus (hpv) infection who underwent colposcopy, 55 yielded histological confirmation of hpv infection and 17 of them had cervical intraepithelial neoplasia (cin). of 51 women followed up six months after cryotherapy of their cervical lesions, 46 showed no evidence of hpv infection in their cervices. of the patients with cin lesions, only one out of the 16 followed up had persistent cin ii six months after treatment, which gave a cl ... | 1988 | 2844652 |
| non-isotopic detection of in situ nucleic acid in cervix: an updated protocol. | recently, sensitive non-isotopic in situ hybridisation (nish) methodology for the detection of human dna and human papilloma virus (hpv) dna in archival paraffin blocks of cervix was described. an amended protocol, now used in this laboratory for detection of these genes by nish is presented. the amendments include the following: protease digestion at 37 degrees c; tissue dehydration in air rather than ethanol; stringency washing in formamide solution; blocking non-specific binding of avidin alk ... | 1988 | 2844861 |
| [detection of human papillomavirus types 16 and 18 dna sequences in the screening of the cells related with carcinoma in situ of the uterine cervix--application of in situ hybridization for cytologic diagnosis]. | in order to investigate the relationship between the presence of human papillomavirus (hpv) dna and cervical carcinoma, we examined the cervical screening cells as well as the biopsy specimens obtained from 3 cases of severe dysplasia, 13 cases of carcinoma in situ (cis) and 2 cases of microinvasive carcinoma for the presence of hpv types 6, 11, 16 and 18 dna by dna-dna in situ hybridization using the biotinylated hpv dna probes. the results of in situ hybridization analysis revealed that hpv 16 ... | 1988 | 2844927 |
| interplay of viral and cellular proteins along the long control region of human papillomavirus type 18. | the long control region of human genital papillomavirus type 18 harbors transcription regulatory elements, such as the e6 promoter and a cell type-specific enhancer independent of the e2 protein. by performing dnase i footprint experiments in vitro with protein extracts from different cell lines and tissues we searched for cellular factors interacting with the totality of the control region. we detected a total of eight different protected sites; most of them were found with all the extracts. tw ... | 1988 | 2845145 |
| a viral-cellular junction fragment from a human papillomavirus type 16-positive tumor is competent in transformation of nih 3t3 cells. | a 4.4-kilobase dna fragment (t4.4) from a human tumor (comprising part of the human papillomavirus type 16 e6 promoter; the e6, e7, and part of the e1 open reading frames; and cellular sequences) was found to be competent to fully transform nih 3t3 cells. this competency resides in the whole hybrid dna fragment, since the separate viral or cellular dna sequences were not active. abundant e6-e7 transcripts were found in the transformed cells. when the cellular fragments were substituted with poly ... | 1988 | 2845153 |
| detection of human papillomavirus in normal and dysplastic tissue by the polymerase chain reaction. | detection of human papilloma virus (hpv) types 16 and 18 in formalin-fixed, paraffin-embedded tissue by a new in vitro dna amplification method, the polymerase chain reaction, was compared with detection with genomic dna probes using in situ hybridization. the polymerase chain reaction replicates exponentially hpv dna sequences present in a single 5- to 10-micron paraffin-embedded tissue section. the amplified sequences are detected with a dna hybridization probe in a dot blot assay. the hpv pol ... | 1988 | 2845193 |
| histologic correlates of papillomavirus infection of the vagina. | vaginal warts (condylomata) secondary to human papillomavirus (hpv) infection are most easily identified when perinuclear halos are seen with clear-cut nuclear atypia (koilocytotic atypia) in the superficial cells of the lesion. however, vaginal lesions clinically resembling condylomata may exhibit lesser degrees of nuclear atypia with findings suggestive of, but not typical for, the presence of hpv. the diagnosis and management of such lesions are unclear. we correlated histologic findings with ... | 1988 | 2845317 |
| high prevalence of genital tract papillomavirus infection in female adolescents. | to investigate clinical condyloma, abnormal cervical cytologic findings, and evidence of human papillomavirus infections, 89 adolescent girls were examined. cellular dnas extracted from exfoliated cervical cells were examined for human papillomavirus genomic sequences by southern transfer hybridization using 32p-labeled human papillomavirus dna probes. human papillomavirus sequences were detected in 12 (13%) young women, abnormal cytologic specimens in 21 (24%), and vulvar condylomas in 12 (13%) ... | 1988 | 2845351 |
| presence of papilloma virus type 11 in condyloma acuminatum of bladder in female renal transplant recipient. | a case is reported of a female renal transplant recipient in whom, after two years of immunosuppression, condylomata acuminata of the genital tract with urethral and bladder extension developed. the condyloma of the bladder was resected endoscopically with no relapse. virologic examination revealed a human papilloma virus type 11. | 1988 | 2845625 |
| functional dissociation of transforming genes of human papillomavirus type 16. | human papillomavirus (hpv) type 16 is thought to be responsible for development of cervical carcinomas, but the mechanism of its carcinogenic action is unknown. to determine which viral genes are involved in cellular transformation, we constructed recombinant murine retrovirus dnas containing various subgenomic fragments of the hpv 16 early region and examined their transforming activities. the results show that the e6 and e7 orfs are the transforming genes of hpv 16; the former governs the tumo ... | 1988 | 2845664 |
| two human papillomavirus dnas molecularly cloned from a patient with epidermodysplasia verruciformis: restriction maps. | two distinct human papillomavirus (hpv) dnas (my-1 and my-2) were molecularly cloned from the benign skin lesions of a patient with epidermodysplasia verruciformis. the restriction map of my-1 was the same as that of hpv 3a. the map of my-2 appeared to be different from those of any hpvs reported in the literature. my-2 did not cross-hybridize with my-1 or the dnas of hpv types 1, 2 and 4 under stringent conditions. | 1988 | 2845712 |
| morphometric assessment of the biological potential of human papillomavirus infections in the uterine cervix. | a series of 69 punch biopsies derived from 513 women prospectively followed up for cervical human papillomavirus (hpv) infections (including hpv lesions with and without cervical intraepithelial neoplasia; hpv-cin, hpv-ncin), and 42 control cases (consisting of normal epithelia, and classical cin lesions) were analyzed morphometrically, using a semiautomatic image analyzer (ibs i-kontron), to assess the value of morphometric measurements in discriminating between hpv lesions and cin, and to find ... | 1988 | 2846018 |
| langerhans' cells and subtypes of human papillomavirus in cervical intraepithelial neoplasia. | there is strong circumstantial evidence that human papillomavirus is a cofactor in the development of cervical neoplasia. systemic immunosuppression has also been implicated. a study was therefore carried out examining the relation between subtypes of human papillomavirus and local immunocompetent cells in the cervix. colposcopically directed punch biopsy specimens were taken from normal cervix and from histologically proved cervical intraepithelial neoplasia for immunohistochemical studies. hum ... | 1988 | 2846100 |
| constitutive and steroid hormone regulated transcriptional enhancer elements in the genome of the human papilloma virus type 16, which is associated with neoplasia of the cervix. | the 7905 bp genomic dna of the human papilloma virus type 16 (hpv-16), which is associated with invasive cervical neoplasia, contains a 832 bp upstream regulatory region (urr) supposed to contain most of its cis-acting promoter elements. a search for dna sequences known to bind transcription factors has resulted in the recognition of numerous consensus sequences. a functional analysis of a segment of this urr containing some of these consensus sequences has resulted in the recognition of an enha ... | 1988 | 2846169 |
| paget's disease of the vulva: search for herpes simplex virus antigens and human papillomavirus antigen and dna. | specimens from three women with paget's disease of the vulva were tested for presence of herpes simplex virus type 2 (hsv2) antigens and human papillomavirus (hpv) antigen and dna. in one of the lesions, the hsv2-associated antigen icsp 34/35 was demonstrated. neither hpv antigens nor hpv dna were detected in the lesions. | 1988 | 2846413 |
| characterization of papillomavirus polypeptides from bovine cutaneous fibropapillomas. | virions of bovine papilloma virus (bpv) were isolated from a pool of cutaneous bovine fibropapillomas and purified by cscl gradient centrifugation. sds-page revealed several polypeptides with an mr ranging from 76k to 19k. western blot analysis of the viral isolate identified additional polypeptides when a rabbit anti-bpv serum was used, but only the main capsid component of 57k when a rabbit antiserum raised against human papillomavirus was used. the viral preparation was then 125i-labelled and ... | 1988 | 2846766 |
| human papillomavirus type 52: a new virus associated with cervical neoplasia. | analysis of biopsies of cervical intraepithelial neoplasia (cin) revealed a high percentage of human papillomavirus (hpv) sequences that would hybridize to a mixture of hpv probes only under conditions of relaxed stringency. the dna sequences of one of these viruses was molecularly cloned and shown to be a new hpv, type 52 (hpv-52). this virus is most closely related to hpv-33. hybridization analysis with restriction fragments of hpv-52 showed collinearity with the hpv-33 genome. dna sequencing ... | 1988 | 2846767 |
| nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus dna sequences. | we examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled dna probes of human papillomavirus (hpv). whole cells were spotted on nitrocellulose filters; dna of the cells was denatured and fixed to the filter. then the dna spots were hybridized to nonradioactive labeled dna and monitored by a sandwich immunoenzymatic reaction. this technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. hpv dna was ... | 1988 | 2846775 |
| expression of human papillomavirus types 16 and 18 dna sequences in cervical carcinoma cell lines. | a total of six cervical carcinoma cell lines, of which two contained human papillomavirus (hpv) type 16 and four contained hpv type 18 dna, were examined for viral rna expression by spot-blot hybridization. all six cell lines contained hpv-specific cytoplasmic poly(a)-containing rna. the same rna species were detected in both cytoplasmic and polysomal fractions, indicating nonselective translation of these messages into protein products. primer extension analysis of rna from the cell lines revea ... | 1988 | 2846777 |
| morphologic and immunohistochemical evidence of human papillomavirus capsid antigen in smokeless tobacco keratoses from juveniles and adults. | seventy-seven tissue samples from patients with either degree i, ii, or iii smokeless tobacco keratoses were examined. the tissues were evaluated for the presence of human papillomavirus (hpv) antigen by immunocytochemical staining. capsid antigen could be identified in sixteen cases (20.78%). this investigation further supports the concept that smokeless tobacco-associated leukoplakias can be correlated with hpv infection. | 1988 | 2846804 |
| induction of anchorage-independent growth of human embryonic fibroblasts with a deletion in the short arm of chromosome 11 by human papillomavirus type 16 dna. | human embryonic fibroblasts with a large deletion (11p11.11p15.1) in the short arm of one chromosome 11 (del-11 cells) appeared to be susceptible to transformation by early human papillomavirus type 16 (hpv-16) dna, whereas diploid human embryonic fibroblasts were not. this difference in susceptibility might be explained by the absence of a tumor suppressor gene located within the deleted part on the short arm of chromosome 11. the presence of abundant viral early-gene transcripts in transformed ... | 1988 | 2846869 |
| transcriptional regulatory elements in the noncoding region of human papillomavirus type 6. | we have identified three elements in the noncoding region of human papillomavirus type 6 (hpv-6) that regulate transcription when assayed in recombinant plasmids containing the bacterial gene for chloramphenicol acetyltransferase. one was a silencer that reduced expression in both a species- and tissue-dependent manner. the second was an enhancer element that was tissue specific. the third was a weak promoter that showed some tissue specificity. these elements have been localized within the nonc ... | 1988 | 2846882 |
| comparison of formalin, buffered formalin, and bouin's fixation on the detection of human papillomavirus deoxyribonucleic acid from genital lesions. | detection of nucleic acid sequences homologous to human papillomavirus (hpv) relies primarily on their extraction from unfixed tissue. we detected hpv sequences in dna extracted from paraffin-embedded tissue fixed in formalin (buffered and unbuffered) and bouin's solution by dot blot hybridization. a detectable hybridization signal was noted in 32% of these fixed tissues which were chosen from cases where hpv dna was detected in the unfixed tissue. when using a homologous 32p-labeled probe and a ... | 1988 | 2846966 |
| glucocorticoid-dependent oncogenic transformation by type 16 but not type 11 human papilloma virus dna. | squamous cell carcinoma of the uterine cervix is one of the most common cancers among women. correlation between human papilloma virus (hpv) infection of the uterine cervix and the development of cervical neoplasia has been established. more recent studies have shown the presence and expression of integrated hpv types 16 and 18 dna sequences in 70-80% of cervical tumours and tumour cell lines. it has been suggested that, in addition to hpvs, other agents such as hormones and tobacco products act ... | 1988 | 2847052 |
| [condylomata acuminata and similar disorders caused by human papillomavirus; clinical and immunological aspects]. | 1988 | 2847063 | |
| [data on the role of the human papillomavirus in the carcinogenesis of the uterine cervix]. | 1988 | 2847101 | |
| penile human papillomavirus infection in consorts of women with genital human papillomavirus infection. a pilot study. | a study determined the incidence of human papillomavirus (hpv) on genitalia of male consorts of women with histologically proven genital hpv lesions to be 50%. | 1988 | 2847335 |
| the biology and significance of human papillomavirus infections in the genital tract. | a variety of human papillomavirus (hpv) types infect the anogenital mucosa, giving rise to lesions that differ in clinical appearance, histology, and risk of malignant progression. certain high-risk types (hpvs 16, 18, 31, 33, 35 and 39) have a strong association with high-grade epithelial neoplasia and invasive carcinomas of the anogenital tract. cancer appears to have a multifactorial etiology, and hpv infection alone is probably insufficient for malignant transformation. the consistent associ ... | 1988 | 2847433 |
| combined herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid leads to oncogenic transformation. | tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation in an immortalized cell line (ae), obtained by transfection of syrian hamster embryo cells with immortalizing ... | 1988 | 2847531 |
| dna ploidy patterns in cervical intraepithelial neoplasia grade iii, with and without synchronous invasive squamous cell carcinoma. measurements in nuclei isolated from paraffin-embedded tissue. | this study presents the results of cytophotometric (cpm) and flow cytometric (fcm) dna ploidy measurements in cervical intraepithelial neoplasias grade iii (cin iii) with and without synchronous invasive squamous cell carcinoma. hysterectomy and biopsy material from 21 patients 35 years of age or younger and from 18 patients age 50 years or older was studied. the dna analysis was performed in nuclei isolated from specific areas of paraffin-embedded tissue. there were significant differences in t ... | 1988 | 2847861 |
| isolation of a new type of human papillomavirus (hpv52b) with a transforming activity from cervical cancer tissue. | substantial evidence has implicated human papillomaviruses (hpvs) as etiological agents of human cervical cancer. we detected and cloned an unidentified hpv genome in cellular dna extracted from a surgical specimen of a japanese patient with cervical cancer. hybridization studies suggested that this hpv was the most homologous to hpv33 among more than 51 types of hpv ever identified. a recently identified new hpv, isolated by w. lancaster and designated hpv52, is also most homologous to hpv33 (w ... | 1988 | 2847864 |
| presence of human papillomavirus type 16 genome in bladder carcinoma in situ of a patient with mild immunodeficiency. | using blot hybridization, we analyzed 10 bladder tumors (1 transitional cell carcinoma in situ, 1 adenocarcinoma, and 8 papillary tumors) for the presence of human papillomavirus (hpv) dna. we detected hpv 16 dna in a transitional cell carcinoma in situ, whereas no hpv dna was found in the other bladder tumors. the patient, a 40-year-old female, who harbored hpv 16 dna in the bladder tumor, had mild immunodeficiency and recently suffered from the bladder tumor, common warts on the right hand, bo ... | 1988 | 2847865 |
| [atypical cervical cancer hyperplasia and human papilloma virus]. | 1988 | 2847904 | |
| squamous papillary neoplasia of the adult upper aerodigestive tract. | selected papillary squamous tumors of the upper aerodigestive tract (uadt) mucosa in adult patients do not have well-defined histologic criteria and the clinical behavior is poorly understood. to better characterize this spectrum of neoplasms, uadt papillary neoplasms were evaluated by routine histology, determination of cellular dna content using feulgen-stained tissue sections, and the typing of human papillomavirus (hpv) by in situ hybridization. solitary papillomas were studied in two patien ... | 1988 | 2847971 |
| pregnancy, human papillomavirus and cervical intraepithelial neoplasia. | human papillomavirus (hpv) has been implicated in the development of cervical intraepithelial neoplasia (cin). to test the hypothesis that the known relationship between hpv and cin might be modified by the altered immune status of pregnancy the cytologic, colposcopic and histologic findings in 176 pregnant and 515 nonpregnant patients referred to a colposcopy clinic were prospectively compared. no difference between the two groups was found regarding the frequency with which cin and hpv were de ... | 1988 | 2838281 |
| pathological incidence study of human papilloma virus (hpv) carried out on 1,439 patients between 1982-1985 in panama. | the author report the results of a study conducted on 1,439 patients screened in the period 1982-1985 in panama. the colposcopic evaluation was based on 1,254 total reports, revealing 705 typical images and pictures and 549 atypical the cytological diagnosis on 766 total reports showed 260 negative, 412 hpv, 75 dysplasia and 19 cancers. the target biopsies registered 433 cases with lesions of which were 93 negative 250 hpv, 111 dysplasia and 19 cancers. the author note an increasing incidence of ... | 1988 | 2838282 |
| papillomaviruses in lesions of the lower genital tract in israeli patients. | human papillomaviruses (hpvs) have been strongly associated with benign lesions of the genital tract (condylomata) and with genital cancer of the vulva and cervix. since the incidence of these lesions in israel is considered to be low, we have studied the presence of hpv 6, 11, 16 and 18 dnas in benign, premalignant and malignant tissue samples or gynecological swabs of the lower genital tract. hpv sequences were detected in 48 out of 66 condylomatous lesions (72%), 5/11 grades i-ii intraepithel ... | 1988 | 2838295 |
| presence of human papillomavirus dna in benign and precancerous oral leukoplakias and squamous cell carcinomas. | a variety of benign squamous epithelial lesions of the mucosa are associated with human papillomaviruses (hpv). recently virus-specific dna was also detected in oral invasive carcinomas. in sections of 373 formalin-fixed and paraffin-embedded samples taken from benign and precancerous leukoplakias and squamous cell carcinomas, hpv dna could be identified in 56 specimens by in situ hybridization technique. the biological significance of viruses of the hpv group in the carcinogenesis of oral epith ... | 1988 | 2838343 |
| subclinical penile human papillomavirus infection and dysplasia in consorts of women with cervical neoplasia. | fifty men whose sexual partners were 50 women with histologically proved cervical intraepithelial neoplasia (cin) grade iii (severe dysplasia or carcinoma in situ) were studied. a further 25 men whose current regular sexual partners were 25 women with chlamydial cervicitis were recruited as controls. if either of the partners in either group had genital condylomata acuminata or a known history of similar lesions, the couple was excluded from the study. abnormal penile epithelium, which was detec ... | 1988 | 2838408 |
| epidermodysplasia verruciformis in a patient with hodgkin's disease: characterization of a new papillomavirus type and interferon treatment. | a new human papillomavirus (hpv) was discovered in disseminated, macular, pityriasis versicolor-like lesions on the skin of the neck, face, scalp, and pubic region of a 42-year-old male suffering from hodgkin's disease. histopathology revealed features characteristic of epidermodysplasia verruciformis (ev). in contrast to classical ev, the lesions were almost exclusively seen in previously irradiated and uv-exposed skin areas. papillomavirus capsid antigen was demonstrated with the genus-specifi ... | 1988 | 2838553 |
| human papillomavirus 8-l1 immune serum: a new diagnostic possibility for epidermodysplasia verruciformis-specific hpvs. | human papillomavirus (hpv) 8 induces flat macular skin lesions with a high risk of malignant conversion. a 600-bp fragment from the center of the major structural protein gene l1 was cloned into pex2 to produce a beta-galactosidase-l1 fusion protein. the expressed part of l1 is masked in intact virions and not detected by sera of infected patients. immunization of guinea pigs with purified fusion protein raised high-titer antisera, which reacted with capsid proteins of hpv8 and closely related v ... | 1988 | 2838555 |
| analysis of the l1 gene product of human papillomavirus type 16 by expression in a vaccinia virus recombinant. | the l1 open reading frame of human papillomavirus type 16 (hpv16) has been expressed in vaccinia virus under the control of both the 7.5k early and late promoter, and the 4b major late promoter. antibodies to a beta-galactosidase fusion protein containing a c-terminal portion of the hpv16 l1 gene product were used to compare the levels of l1 expression in the two recombinants, and showed that greater levels of expression were obtained when the gene was placed under the control of the 4b late pro ... | 1988 | 2838573 |
| transformation of rat 3y1 cells by a deletion dna of human papillomavirus type 16 molecularly cloned from genomic dna of a cervical carcinoma. | human papillomavirus type 16 (hpv 16) dna integrated within the cell dna in cervical carcinomas is frequently defective, but commonly retains its putative transcriptional control region and e6 and e7 open reading frames (orfs) intact. one clone of such a partially deleted hpv 16 dna was tested and found to be transforming for rat 3y1 cells. the results indicates that hpv 16 dna containing e6 and e7 orfs with the homologous promoter is sufficient for inducing focal transformation of immortalized ... | 1988 | 2838580 |
| need for national register to monitor cervical human papilloma virus infection. | 1988 | 2837708 | |
| human papillomavirus dna in adenosquamous carcinoma and squamous cell carcinoma of the vulva. | the tissues from 16 cases of adenosquamous carcinoma (pseudoglandular squamous cell carcinoma or adenoacanthoma of the sweat glands of lever) and 26 cases of invasive squamous cell carcinoma of the vulva were studied for the presence of human papillomavirus (hpv) genomes using southern blot hybridization on fresh tissues. types 1, 2, 3, 4, 5, 6, 16, and 18 hpv dna probes and in situ hybridization were used on formalin-fixed paraffin sections using type 2, 6, 16, and 18 hpv dna probes. only one c ... | 1988 | 2837709 |
| condylomatous carcinoma of the vulva with special reference to human papillomavirus dna. | nine cases of condylomatous carcinoma (squamous cell carcinoma arising in condyloma acuminatum) of the vulva were studied for their clinical history, histopathology, and presence of human papillomavirus (hpv) dna. condylomatous carcinoma occurred primarily in an elderly population with a mean age of 70 years. there was an antecedent history of vulvar condyloma in 77%, with a median of nine months before the documentation of an invasive lesion. the disease had a good prognosis, with few recurrenc ... | 1988 | 2837710 |
| correlation of cytologic, colposcopic, and histologic studies with immunohistochemical studies of human papillomavirus structural antigens in an unselected patient population. | a study was undertaken to determine the prevalence of human papillomavirus infection in the general population. the first phase evaluated cytologic and colposcopic diagnoses in 962 consecutive patients in a community practice. the second, detailed phase evaluated 110 consecutive patients making routine visits in the same practice. studies performed for each patient in phase two were cervical cytology, cervical cytologic immunohistochemistry for human papillomavirus, colposcopy with biopsy of all ... | 1988 | 2837901 |
| sensitivity of in situ detection with biotinylated probes of human papilloma virus type 16 dna in frozen tissue sections of squamous cell carcinomas of the cervix. | the sensitivity of human papilloma virus type 16 (hpv-16) dna detection by dna in situ hybridization using biotinylated probes (bio-dish) was estimated by performing this technique on snap-frozen tissue sections of 10 cervical squamous cell carcinomas containing increasing amounts of hpv-16 as determined by southern blot hybridization. a protocol using serial sections for bio-dish and dna extraction was used. the number of positively stained cells and the detection limit were strongly dependent ... | 1988 | 2837906 |
| immunohistochemical identification of human papillomavirus infection in tissues from cervical precancerous and early cancerous lesions among indian women. | paraffin sections of 50 cervical condyloma biopsies from patients with precancerous and early cancerous lesions of the uterine cervix were screened for human papillomavirus (hpv) antigen by peroxidase-antiperoxidase (pap) staining using genus specific anti-bovine papillomavirus serum (rabbit). the intra-nuclear hpv positivity was observed in 20 percent (10/50) of biopsies studied. further, proportionately larger number of cases with moderate dysplasia (cin ii) associated with koilocytotic change ... | 1988 | 2837927 |
| solitary condylomatous papilloma of the bronchus. | two case reports of solitary bronchial papillomas are presented. the histologic features of the lesions are those of squamous papillomas exhibiting prominent condylomatous changes. immunoperoxidase stains revealed the presence of human papillomavirus antigens in the koilocytotic cells. ultrastructural studies demonstrated possible intranuclear viral-size particles. solitary condylomatous papillomas of the bronchus should be distinguished from solitary bronchial squamous papillomas that do not ha ... | 1988 | 2838006 |
| verrucous carcinoma of the vulva containing human papillomavirus-11. case report. | 1988 | 2838068 | |
| regional chromosome localization of human papillomavirus integration sites near fragile sites, oncogenes, and cancer chromosome breakpoints. | the integration sites of human papillomavirus (hpv) dna within the cervical carcinoma cell line c4-i and a primary cervical tumor were mapped by in situ hybridization. cloned cellular sequences flanking the integrated viral dna were used as probes. for the cell line, the viral integration site was mapped to chromosome region 8q21-q22.3, while in the primary tumor chromosome band 3p21 was the target for integration. the hpv dna integration appears to occur in the vicinity of fragile sites, oncoge ... | 1988 | 2838160 |
| identification of immunoreactive antigens of human papillomavirus type 6b by using escherichia coli-expressed fusion proteins. | human papillomavirus (hpvs) infect the genital epithelium and are found in proliferative lesions ranging from benign condylomata to invasive carcinomas. the immunological response to these infections is poorly understood because of the lack of purified viral antigens. in this study, bacterially derived fusion proteins expressing segments of all the major open reading frames (orfs) of hpv type 6b (hpv-6b) have been used in western blot (immunoblot) assays to detect antibodies directed against hpv ... | 1988 | 2835513 |