Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| cytologic detection of human papillomavirus infection. | in recent years there has been a dramatic increase in the incidence of human papillomavirus infection of the female genital tract. since the primary diagnosis of this condition is almost invariably based on cytologic observations, the diagnostic criteria are of paramount importance. the diagnosis is usually based on the presence of koilocytes, or balloon cells, and dyskeratotic cells. although these cells remain fundamental to the diagnosis, it is important to recognize other possible cytologic ... | 1987 | 2829079 |
| integration and transcription of human papillomavirus type 6 recombinant dna in mouse cells. | human papillomavirus (hpv) dna is found in nature mostly in an episomal form. however, in permanent cell lines established from cervical carcinomas in which hpv sequences are present, they are usually integrated in the host genome. in vitro studies of hpv have been hampered because of the difficulty of stably maintaining hpv sequences in transfected cells. we have cloned the entire hpv6 genome into three vectors: pml2, a derivative of pbr322 lacking the "poison sequences"; p142-6, a plasmid cont ... | 1987 | 2829459 |
| genital human papillomavirus infections and cancer: memorandum from a who meeting. | cervical cancer ranks as the 2nd most frequent cause of cancer in women. research demonstrates that infection with the human papilloma virus (hpv) leads to cervical cancer. the clinical hpv lesion in both sexes is the pointed wart like tumor called condyloma acuminatum. detecting subclinical lesions varies, however, based on the genital organ and on the methods of examination. several types of hpv infect anogenital epithelia and the resultant disease is partially determined by hpv type. in ... | 1987 | 2830042 |
| a study on the relationship between human papillomavirus infection and cervical carcinoma development. immunoperoxidase localization of papillomavirus antigen in cervical tissue. | 1987 | 2832133 | |
| [genital papillomavirus lesions. coexistence in sexual partners, role in cancer of the cervix and value of colposcopy]. | since the human papillomavirus is transmissible sexually, an ever increasing number of male patients is seen in dermatology departments. it therefore seems to be important to evaluate the frequency of hpv-related genital lesions in both males and females. several recent studies have made it clear that concomitant lesions are very often observed in sexual partners. we have tried here to summarize the different data reported in the literature concerning the frequency of hpv lesions and their proba ... | 1987 | 2829688 |
| role of herpes simplex virus type-2 and human papilloma virus in penile cancers in singapore. | to detect the presence of herpes simplex virus type 2 (hsv-2) and human papilloma virus (hpv), common structural antigens in penile cancers, 20 squamous cell carcinomas were studied by immunohistochemical methods. all specimens were from singaporeans ranging in age from 44 to 69 years. none of the specimens contained hsv-2 and hpv common structural antigens. the reasons for the absence of hpv common antigen were discussed. the significance of the association of hpv with penile cancers is uncerta ... | 1987 | 2829697 |
| human papillomavirus. | 1987 | 2829073 | |
| clinical manifestations and natural history of genital human papillomavirus infection. | over the past decade, the ever-increasing volume of evidence implicating hpv types in genital neoplasia has stimulated much research interest into all aspects of the biology of this interesting group of viruses. this has led to the identification of an ever-increasing heterogeneity of hpv types. it is not surprising, therefore, that the clinical profile of disease associated with genital hpv types is much broader than previously recognized. knowledge of this clinical spectrum is mandatory to th ... | 1987 | 2829075 |
| genital human papillomavirus infection. a growing concern. | hpv infections of the genital tract are one of the most common sexually transmitted viral infections in the united states. data from std clinics and private physicians' offices reveal that genital warts, one manifestation of genital hpv infection, have been diagnosed more frequently in recent years. using a variety of diagnostic techniques, asymptomatic hpv infection has been identified in men and women and is probably much more common than clinically apparent infection. | 1987 | 2829076 |
| detection of human papillomavirus infection by nucleic acid hybridization. | single-stranded nucleic acid molecules that are complementary to each other will form hybrids under appropriate conditions. hybridization tests make use of this phenomenon and employ labeled molecules called probes to detect specific complementary molecules called targets. nucleic acid hybridization is the most sensitive method for detecting hpv in clinical specimens and the only one capable of identifying specific hpv types. there are many alternative hybridization test formats; most of them us ... | 1987 | 2829080 |
| human papillomavirus infection and anogenital neoplasia: speculations for the future. | hpv infections of the female genital tract are common. the association of these viruses with anogenital neoplasia has stimulated efforts to devise practical methods of detection and typing of hpv. although experimental diagnostic tests are available, they are, for the most part, complex and time consuming and are limited to medical centers researching hpv. new methods for preparing probes with higher sensitivities for hybridization tests will allow use of in-situ methods on formalin-fixed tissue ... | 1987 | 2829087 |
| natural history of human papillomavirus infection of the anogenital tract. | a number of viruses, most notably herpes virus type 2, have been suggested as etiological agents of cervical neoplasia. recent studies with human papillomaviruses, however, have demonstrated a remarkable association of a subgroup of these viruses with about 90% of benign, preinvasive and invasive lesions of the cervix and anogenital tract. the oncogenic potential of papillomaviruses has been demonstrated both in laboratory animals and in cultured cells. furthermore, susceptibility to certain hum ... | 1987 | 2832090 |
| transcription of human papillomavirus type-18 dna in human cervical carcinoma cell lines. | 1987 | 2832273 | |
| human papillomavirus type 2 dna in oral and labial verruca vulgaris. | twenty instances of verruca vulgaris, equally divided between oral mucosa and lip vermillion, were assayed for the presence of human papillomavirus (hpv) capsid antigen and type-specific dna employing in situ hybridization methods. all 10 cases arising on lip vermillon expressed capsid antigen and harbored hpv type 2 genomes as assessed under conditions of high stringency dna hybridization. oral verrucae rarely expressed capsid antigen; hpv type 2 genomes were encountered in 20% of the cases. | 1987 | 2832456 |
| cervical smears in assessment of the natural history of human papillomavirus infections in prospectively followed women. | the value of cervical (papanicolaou) smears in monitoring the natural history of cervical human papillomavirus (hpv) infections was assessed in a series of 513 women prospectively followed since 1981. on each clinic visit, the patients were subjected to colposcopy accompanied by cervical smears and/or punch biopsies. the latter were analyzed by light microscopy for concomitant cervical intraepithelial neoplasia (cin) and by transmission electron microscopy (tem) for hpv particles as well as for ... | 1987 | 2827414 |
| [gene molecular and ultrastructural studies on the relationship between human squamous epithelial carcinoma of the uterine cervix and the human papilloma virus]. | 1987 | 2832086 | |
| relationship between human papillomavirus infection and uterine cervical carcinoma development. a pathomorphological observation of 357 cases. | 1987 | 2834145 | |
| increased incidence of cervical intraepithelial neoplasia grade 3 and human papillomavirus infection at a family planning clinic. | a five-fold increase in the incidence of histologically-proven cervical intraepithelial neoplasia grade 3 (cin3) was observed for patients attending the christchurch family planning clinic in 1985 compared with the preceding three years. there were 51 new cases in 1985 and a yearly average of 9.3 between 1982 and 1984. there was an increase also in cervical smears showing evidence of human papillomavirus (hpv) infection. the time interval between the last normal smear and the diagnosis of cin3 w ... | 1987 | 2837702 |
| venereal papilloma and papillomavirus in a colobus monkey (colobus guereza). | a papilloma on the penis of a colobus monkey was found to contain papilloma-virus group specific antigens by immunohistochemical analysis and virus-like particles in the nuclei of epithelial cells by transmission electron microscopy. in low-stringency southern blot hybridizations, dna from the lesion annealed with human papillomavirus 11 dna, but not with the dnas of 13 other papillomaviruses. using human papillomavirus 11 dna as a probe in southern blot hybridizations, dna from the penile papil ... | 1987 | 2835331 |
| [human papillomavirus infection and carcinogenesis of cancer of uterine cervix. ii. immunoperoxidase localization of papilloma-virus antigen in tissues of the uterine cervix]. | 244 cervical tissue specimens with different diagnoses were stained by peroxidase-anti-peroxidase technique (pap) for assay of the human papillomavirus (hpv) genus-specific antigen, the first of its kind performed in china. hpv antigen was detected in 41.93% of simple condyloma group, in 35.29% of dysplasia with condyloma and in 27.27% of carcinoma with condyloma. there was no positive in the control, dysplasia or carcinoma groups. the antigen positive nuclei were found in the koilocytes or para ... | 1987 | 2838240 |
| [relation between human papillomavirus (hpv) and cancer of uterine cervix]. | hpv-dna fragments were detected in biopsy specimens (29 cases of cancer of uterine cervix, 2 cervical dysplasia and 9 normal cervix) using dna hybridization technique. it was demonstrated that 52% of biopsy specimens of the cancer of uterine cervix was positive for hpv 16 dna probe, while 9% was positive for hpv 18 dna probe. 11% of non-cancerous biopsy specimens had a positive result for hpv 16 dna probe. it was also demonstrated that the positive rate of hpv 16 dna was 75% in grossly cauliflow ... | 1987 | 2838241 |
| a method of in situ molecular hybridization applied to the study of viral papillomas in man. | thirty nine papillomas and 14 precancerous lesions, from skin and mucosa were studied for the presence of human papillomavirus (hpv) infection on frozen sections or on paraffin embedded sections by comparison of 2 methods: (1) detection of group specific viral antigen by immunohistochemical techniques with a rabbit antiserum raised to highly purified virus dissociated by sds (sodium dodecylsulfate) and heating: (2) detection of viral dna by an in situ molecular hybridization technique with bioti ... | 1987 | 2833145 |
| human papillomavirus (hpv) and its role in oral disease--an overview. | 1987 | 2852565 | |
| human papillomavirus dna in bronchial squamous cell carcinomas. | 1987 | 2880009 | |
| human papillomavirus type 5 and skin cancer in renal allograft recipients. | 1987 | 2885608 | |
| prognosis of women with human papillomavirus dna in normal tissue distal to invasive cervical and vulval cancer. | 1987 | 2881111 | |
| human papillomavirus and cervical cancer. | 1987 | 2882384 | |
| human papillomavirus dna and oral mucosa. | 1987 | 2880158 | |
| [cytology in human papilloma virus (hpv) infections: is it possible to fix cytologic standards to determine the timing of activation?]. | 1987 | 2851547 | |
| molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 dna. | a proliferating population of human foreskin keratinocytes (presently in the sixtieth passage) has been obtained after transfection with human papillomavirus (hpv) type 16 dna. in contrast, the control cultures did not survive beyond the sixth passage. cytogenetic analysis of cells taken from the twelfth passage revealed a heteroploid male karyotype. in approximately 50% of the cells a common marker chromosome was found, suggesting a clonal origin for at least part of the population. this is fur ... | 1987 | 2838778 |
| genital human papillomavirus infections. | in summary, the development of new techniques to identify hpv dna in genital secretions and tissue, the recognition of subclinical hpv infection, and the remarkable association between hpv and genital neoplasia have markedly increased the concern of both patients and physicians about genital wart virus infections. the prevalence of this viral std appears to be increasing and the clinical spectrum of disease appears to be expanding. new methods to diagnose genital hpv infection and techniques to ... | 1987 | 2848068 |
| the natural history of human papillomavirus (hpv) atypia of the cervix. | this study examines the natural history of human papilloma virus (hpv) atypia of the cervix in 259 untreated patients who were followed for 3 to 74 months (median 18 months). progression to cervical intraepithelial neoplasia (cin) occurred in 41 patients (15.8%)--all but 3 progressions occurred within the first 24 months of follow-up. in no patient did invasive cancer develop during this study. persistence occurred in 39.4% and regression in 44.8% patients. an increasing number of regressions we ... | 1987 | 2840059 |
| multifocal invasive carcinoma of the vulva in a 25-year-old woman with bowenoid papulosis. | this case report documents malignant progression associated with wart virus infection of the vulva in a 25-year-old female. the initial condition of bowenoid papulosis and carcinoma in situ of the vulva was diagnosed on colposcopic biopsies performed to investigate chronic pruritus and superficial dyspareunia. this condition failed to resolve with local ablative therapy and progressed over a period of 8 months to multifocal invasive carcinoma of the vulva requiring radical surgery. deoxyribonucl ... | 1987 | 2840060 |
| a human papilloma virus type 11 transcript encoding an e1--e4 protein. | the human papilloma virus (hpv) associated with a genital wart (condyloma acuminatum) was determined to be type 11. the majority of the viral dna molecules were monomeric circles present in the cells at high copy number, as demonstrated by one- and two-dimensional agarose gell electrophoretic separation followed by southern blot analysis. a cdna library in phage lambda gt11 was constructed from poly(a)-selected mrna recovered from the tissue. recombinant clones corresponding to the most abundant ... | 1987 | 2887066 |
| human papillomavirus frequency in normal cervical tissue. | 1987 | 2887806 | |
| human papillomavirus infections in women with and without abnormal cervical cytology. | 9295 smears, obtained from women attending three gynaecological hospitals for routine screening, were examined for human papillomavirus (hpv) types 6 and 11 and hpv 16 and 18 infections by filter in-situ hybridisation. the data were compared with cytological findings. in women with normal cytological smears hpv infection was identified in about 10% of women aged between 15 and 50 years and in less than 5% of those aged over 50. in women with abnormal smears (cervical intraepithelial neoplasia [c ... | 1987 | 2888940 |
| identification and mapping of human papillomavirus type 1 rna transcripts recovered from plantar warts and infected epithelial cell cultures. | multiple spliced transcripts of human papillomavirus type 1 were detected by electron microscopic analysis of r-loops formed with total rna extracted from plantar warts and with poly(a)+ rna isolated from cultured keratinocytes infected with human papillomavirus type 1. the 5' ends of the rnas were mapped to sites in the e7 open reading frame (orf), just upstream of the e6 orf and in the upstream regulatory region. species with 5' ends in e7 accounted for over 95% of all transcripts seen. two po ... | 1987 | 2883327 |
| human papillomavirus type 16 dna in anal squamous cell carcinoma. | 1987 | 2885526 | |
| bowenoid papulosis. | bowenoid papulosis is a recently described condition with a clinically benign appearance of multiple reddish-brown or violaceous papules. the histologic picture, however, is that of squamous-cell carcinoma in situ. in the reported cases the lesions usually occurred on the genitalia of patients in the third or fourth decades of life. we recently encountered a patient who presented with papules in the anal area. human papillomavirus has been identified as the causative agent. differentiation from ... | 1987 | 3026758 |
| characterization of a transcriptional promoter of human papillomavirus 18 and modulation of its expression by simian virus 40 and adenovirus early antigens. | rna present in cells derived from cervical carcinoma that contained human papillomavirus 18 genomes was initiated in the 1.053-kilobase bamhi fragment that covered the complete noncoding region of this virus. when cloned upstream of the chloramphenicol acetyltransferase gene, this viral fragment directed the expression of the bacterial enzyme only in the sense orientation. initiation sites were mapped around the atg of open reading frame e6. this promoter was active in some human and simian cell ... | 1987 | 3023691 |
| a new type of human papillomavirus associated with oral focal epithelial hyperplasia. | lesions from 10 patients suffering from focal epithelial hyperplasia (feh) of the oral mucosa, including those of 4 greenlandic eskimos, were investigated for the presence of human papillomavirus (hpv) dna sequences by blot hybridization experiments. two distinct hpvs were detected in the dna extracted from these lesions, and their genomes were molecularly cloned and characterized. one of these hpvs, detected in 4 patients, was found to be identical with hpv13, whose association with feh was alr ... | 1987 | 3027189 |
| laboratory production in vivo of infectious human papillomavirus type 11. | human papillomaviruses (hpv) induce among patients natural lesions which produce small amounts of virus. infection of human cell cultures does not lead to the multiplication of virus, which also does not replicate in experimental animals. we have developed a unique system for the laboratory production of hpv type 11 (hpv-11). fragments of human neonatal foreskin were infected with an extract of naturally occurring human vulvar condylomata and grafted beneath the renal capsule of athymic mice. la ... | 1987 | 3027386 |
| biologic course of cervical human papillomavirus infection. | to determine the natural course of cervical human papillomavirus infection, we prospectively studied all new patients referred to the colposcopy clinic at the naval hospital bethesda, from april 1981 to august 1983, whose screening cervical cytology demonstrated features consistent with human papillomavirus infection as the only abnormality. histologic confirmation of human papillomavirus infection was required for entry into the study. all patients were evaluated by repeat cytology, colposcopy, ... | 1987 | 3027635 |
| [human papillomavirus infections of the female genital tract]. | 1987 | 3027876 | |
| human papillomavirus type 13 dna in focal epithelial hyperplasia among mexicans. brief report. | human papillomavirus (hpv) type 13 dna was detected in focal epithelial hyperplasia lesions of the oral mucosa in seven half-caste mexicans. the lesions contained intracellular papillomavirus-like particles with a diameter of about 50 nm. dna extracted from biopsies contained unintegrated hpv type 13 dna genomes as revealed by southern blot hybridization. the hpv 13 dna that was isolated in the present study had the same restriction enzyme cleavage map as hpv 13 dna, previously described by othe ... | 1987 | 3028340 |
| a survey of human cancers for human papillomavirus dna by filter hybridization. | we examined 217 tissue samples of various human malignancies for the presence of human papillomavirus (hpv) dna using low-stringency filter hybridization techniques. these techniques were sufficiently sensitive for crosshybridization of the hpv dna probes to all the known types of papillomavirus dnas, both human and animal. approximately 2% of the cancers analyzed contained hpv dna. these included carcinomas of the lung, cecum, tongue, and neck. three of four cancers contained hpv-16-related nuc ... | 1987 | 3024803 |
| detection of human papillomavirus dna in invasive carcinomas of the cervix by in situ hybridization. | an examination of 27 invasive cancers of the cervix was performed using the technique of in situ hybridization using human papillomavirus dna probes. four tissues, previously found to harbor papillomavirus dna by filter hybridization, were confirmed by in situ analysis. one further tissue never previously studied was also found to be positive by in situ hybridization. overall, we found 33% of invasive cancers of the cervix to contain human papillomavirus dna. in contrast, 55% of carcinoma in sit ... | 1987 | 3024824 |
| colposcopy. | colposcopy was introduced to canadian medical practice in the late 1960s and its use has only recently become widespread. until the advent of colposcopy, most women with abnormal pap smears had a cone biopsy of the cervix with all its attendant problems. the colposcope has completely changed the management of an abnormal pap smear with respect to diagnosis and has had a remarkable influence on treatment. cryosurgery and carbon dioxide laser surgery were introduced as out-patient treatments for p ... | 1987 | 21263978 |
| laryngeal papilloma cells in culture have an altered cytoskeleton. | we have investigated the effect of human papillomavirus (hpv) transformation on cellular cytoarchitecture. cells from laryngeal papillomas and normal epithelium were cultured in vitro. cytoskeletal components of both types of cells were visualized by immunofluorescence, to determine whether there were any differences in the structure or distribution of the cytoskeleton. there was no significant change in microtubules. two major components of the cytoskeleton, the intermediate filaments and the m ... | 1987 | 21449663 |
| sensitive detection and typing of human papillomavirus dna in gynecological cell scrapings by slot-blot hybridization. | a rapid and sensitive method for detecting and typing human papillomaviruses (hpvs) in cell scrapings is presented. dna from scrapings is extracted and bound to nitrocellulose filters (slot-blot). by dna-dna hybridization with specific 32p-labelled hpv-probes (types 6/11 or 16/18) the patient's dna is then analyzed for the presence of, and for the type of, hpv dna sequences. a parallel hybridization with a human repetitive element (alu sequence) allows quantitation of the different hybridization ... | 1988 | 2907205 |
| [detection of human papillomavirus sequences in uterine cervix lesions in venezuela]. | 1988 | 2855187 | |
| rearrangement of both alleles of human chromosome 8 in hela cells, one of them as a result of papillomavirus dna integration. | integration of papillomavirus in the genome of the host cell has been found associated with malignant cases of cervical carcinoma. to determine what role viral integration plays as part of the pathogenic mechanism resulting in a cancer cell, the structure of integrated papillomavirus dna (human papillomavirus dna 18) segments and its cellular flanking sequences in hela cells as well as the corresponding normal human allele have been characterized. all integrated viral dna segments have the same ... | 1988 | 2891697 |
| human papillomavirus preceding intraepithelial neoplasia in serial cervical smears. | 1988 | 2896842 | |
| absence of cytomegalovirus, epstein-barr virus, and papillomavirus dna from adenoma and adenocarcinoma of the colon. | biopsy specimens from 13 patients with adenocarcinoma of the colon and from 10 patients with endoscopic polypectomies for colon adenoma were examined for the presence of the dna of cytomegalovirus (cmv), epstein-barr virus (ebv), and human papillomavirus (hpv) types 2, 6, 16 and 18. the specific activities of viral dna probes obtained by nick--translation ranged from 10(7) to 10(8) cpm/micrograms dna. by southern blot hybridization with an estimated sensitivity of 10 pg virus dna which correspon ... | 1988 | 2903634 |
| [study of immunologic parameters in parenteral drug addicts (pda) with condylomata acuminata]. | a study is made of the state of cell and humoral immunity in parenteral drug addicts both with and without genital warts. the results reveal alterations in the lymphocyte subpopulations, with a decrease in the t-helper/t-suppressor (t4+/t8+) ratio of both groups of patients. these alterations were more pronounced in those patients with genital warts (p less than 0.05). the alterations in humoral immunity, expressed as a hypergammaglobulinemia igg and igm, were similar in both groups. none of the ... | 1988 | 2904516 |
| sensitive and quick test for human papilloma virus. | 1988 | 2856324 | |
| [cervical intraepithelial neoplasm and human papilloma virus]. | 1988 | 2856453 | |
| tolerance and efficacy of recombinant human interferon gamma in the treatment of refractory genital warts. | the intralesional administration of recombinant interferon alpha has led to resolution of genital warts in 50 to 70 percent of cases. however, continuous warts do not respond to such treatment, and human papillomavirus (hpv) infection of other anatomic sites remains untreated, so infection may continue to be transmitted. since interferons have shown promise as effective therapies for genital warts, we decided to investigate the tolerance and efficacy of recombinant human interferon gamma in the ... | 1988 | 2840824 |
| future trends in cervical cancer. | the identification of the close association of certain types of human papillomavirus with the development of cervical cancer should lead to an extensive revision of appropriate health policies. having taken into account the drawbacks inherent in the existing data (stemming from the use of varying nomenclature, diagnostic methods and reliability, registration and screening practices) it is possible to conclude that the incidence of hpv infections, all premalignant and malignant stages of cervical ... | 1988 | 2841018 |
| transcriptional activity of human papillomavirus type 6 in respiratory tract papillomata. | we have investigated the molecular basis for differences that we observed in the biological activities of genetically related but distinguishable human papillomavirus type 6 (hpv-6) subtypes. to analyse tissue-specific differences in replication and transcription, and to identify viral gene products important in the benign transformation of epithelial cells, we have modified procedures utilizing guanidinium isothiocyanate and density gradient centrifugation to facilitate the extraction of relati ... | 1988 | 2839601 |
| isolation and characterization of a variant of human papillomavirus type 11 from a nasal inverting (schneiderian) papilloma. | we have previously reported the presence of a variant of human papillomavirus (hpv) type 11 in a nasal inverting papilloma [respler et al., 1987]. in the present study, we have cloned molecularly the dna of this variant at its unique restriction enzyme bam hi site into lambda bf101 phage. restriction enzyme mapping and dna sequencing revealed that the genome of this virus contained an extra 531 base pair (bp) which was the repeat of most of the noncoding region (ncr) of hpv 11. insertion of tran ... | 1988 | 2839608 |
| alterations in the regulatory region of the human papillomavirus type 6 genome are generated during propagation in escherichia coli. | we analyzed the long control regions (lcrs) of seven human papillomavirus type 6b (hpv-6b) clones, which contained prototype hpv-6b sequences recloned into various plasmid vectors and propagated in different strains of escherichia coli. southern blot analysis and dna sequencing demonstrated three different sequences, each distinct from the published prototype hpv-6b sequence. two of the plasmids contained insertions of 24 and 94 base pairs (bp) and a 1-bp deletion. four plasmids contained insert ... | 1988 | 2841482 |
| [co2 laser therapy and typing of human papilloma virus in follow-up studies of cervix intraepithelial neoplasms]. | in 104 woman patients with cervical intraepithelial neoplasia (cin) i-iii who had been treated between 1981 and may 1986 in the dysplasia consulting-room of the department of gynecology by means of conisation effected by laser, we checked on therapeutic success via cytology and colposcopy as well as by human papilloma virus (hpv) typing. in 90.3% of the cases 1.25 laser treatments sufficed for healing of the lesion. relapses occurred mainly in severe intraepithelial neoplasias with identified hp ... | 1988 | 2840323 |
| anogenital warts of the condyloma acuminatum type in hiv-positive patients. | anogenital warts of the condyloma acuminatum type seem to occur quite often during hiv infection. these warts--according to our study--are not commonly caused by malignancy-associated human papilloma virus types, but by types 6 and 11 as seen in the nonimmune-compromised population. widespread condylomata acuminata may appear in rather early stages of hiv infection and they may therefore represent early warning signs of hiv infection. | 1988 | 2841174 |
| transformation of primary human fibroblast cells with human papillomavirus type 16 dna and ej-ras. | human papillomavirus type 16 (hpv type 16) has been implicated as an etiological agent in human cervical cancer. this virus contains sequences which, under the proper transcriptional control, can increase the tumorigenicity of established mouse cells and cooperate with ej-ras in transforming primary baby rat kidney (brk) cells. these data argue that this virus contains oncogenic sequences. because this is a human virus, it was important to study the effect on primary human cells of hpv type-16 d ... | 1988 | 2841248 |
| [ultrastructural observation of human papillomavirus particles in the uterine cervix intraepithelial neoplasia]. | using transmission electron microscopy (tem), twenty two cases of an intraepithelial lesion in the uterine cervix have been examined for the presence of human papillomavirus (hpv) particles. in 21 of these cases (95%), hpv particles were detected in the nucleus, and in 5 cases, in the cytoplasm. the distribution of the intranuclear hpv particles was classified into 4 types. in only 2 cases did the exhibited particles show a geometrical crystalline array (type i). in most cases, the exhibited par ... | 1988 | 2841511 |
| adenocarcinoma of the cervix associated with human papillomavirus. | eleven pure adenocarcinomas of the cervix were analyzed for human papillomavirus (hpv) types 6, 11, 16, 18 and 31 by southern blot hybridizations. seven tumors were positive for papillomavirus dna. five hybridized to the hpv 18 specific probe and two tumors hybridized to hpv 16. all tumors were negative for hpv 6/11 and 31, and no additional tumors became positive with hybridizations under nonstringent conditions to a mixture of hpv probes. no papillomavirus dna was detected in 11 noncervical ma ... | 1988 | 2843277 |
| expression of human papillomavirus type 6b e2 gene product with dna-binding activity in insect (bombyx mori) cells using a baculovirus expression vector. | human papillomavirus type 6b (hpv6b) has been shown to be a major etiologic agent of genital condylomas. the e2 gene, one of the early genes, has been shown to activate the enhancer element in trans in cells transformed with bovine papillomavirus type 1a (bpv1a) but the e2 gene product of any hpv has not been identified. the e2 gene of hpv6b was inserted into the polyhedrin gene of a baculovirus, bombyx mori nuclear polyhedrosis virus (bmnpv), 156 bp downstream from the translational start codon ... | 1988 | 2842232 |
| synthetic oligonucleotide probes for the detection of human papilloma viruses by in situ hybridisation. | using the published nucleotide sequence data for human papilloma virus (hpv) types 1, 6 and 16, sequences of 30 bases in length from the beginning of the e6 open reading frame (orf) were selected. oligonucleotides were synthesised on an oswel gene synthesizer and labelled at the 3' end with biotin using the enzyme terminal transferase. in situ hybridisation was carried out on paraffin sections of wart and cervical tissues mounted on silanated slides. a 2 h hybridisation step allowed the whole pr ... | 1988 | 2842356 |
| analysis of individual human papillomavirus types in cervical neoplasia: a possible role for type 18 in rapid progression. | histologic and molecular analyses of 214 cervical biopsy specimens were performed to test the hypothesis that certain individual human papillomavirus types that are usually grouped together are differentially distributed in various grades of cervical intraepithelial neoplasia and invasive squamous carcinoma. specifically, types 16 and 18, which are commonly grouped together, were analyzed separately and compared. biopsies obtained from three different geographic sites in the united states and br ... | 1988 | 2841858 |
| human papillomavirus-16 and genital cancer: are tests for the viral gene expression in vitro indicators for risk factors in vivo? | human papillomaviruses are found in up to 90% of all cervical carcinomas and are considered to play a causal role in the etiology of this malignancy. the genome of human papillomaviruses consists of a single circular dna molecule with a size of approximately 8000 basepairs. 90% of this genome encodes proteins involved in functions such as neoplastic transformation of the host cell or formation of the viral capsid. the remaining 10% of the genome, which is termed upstream regulatory region (urr), ... | 1988 | 2841899 |
| prevalence of genital hpv infections in a regularly screened population in the netherlands in relation to cervical cytology. | to determine the prevalence of human papilloma virus (hpv) genotypes in relation to cervical cytology, 1,290 cervical samples from a regularly screened population of 30-55-year-old women were investigated. gynaecological specimens, obtained from the cervix, were cytologically classified and screened for the presence of hpvs 6/11 and 16/18 using dot-spot dna hybridisation. of the cervical samples containing unequivocally normal cells, 21 of 1,271 (1.6%) were found positive for hpv, and of the cer ... | 1988 | 2842442 |
| prospects for human papillomavirus vaccines and immunotherapies. | 1988 | 2840515 | |
| involvement of human papillomavirus type 8 genes e6 and e7 in transformation and replication. | we investigated the transforming activity of human papillomavirus type 8 (hpv8) by expressing all early open reading frames from a heterologous promoter in different rodent fibroblast lines. morphological transformation was observed only in g418-selected mouse c127 and rat 1 cells containing an intact e6-coding region. e6 of hpv8 did not transform nih 3t3 cells as did e6 of bovine papillomavirus type 1. c127 cells transformed by e6 were anchorage independent and had a reduced serum requirement b ... | 1988 | 2843666 |
| correlation of histology with human papillomavirus dna detection in the female genital tract. | genital condyloma and intraepithelial neoplasia secondary to human papillomavirus (hpv) infection are characterized by perinuclear halos and marked nuclear atypia (koilocytotic atypia) on cytologic and histologic examination. however, at times the histologic findings, including the degree of nuclear atypia, may be suggestive but not absolutely diagnostic of an hpv related neoplasm. hpv dna sequences were detected in 63 and 56% of colposcopically visible vaginal and cervical lesions, respectively ... | 1988 | 2842237 |
| association of human papillomavirus with vulvodynia and the vulvar vestibulitis syndrome. | seven women presenting with longstanding introital dyspareunia and burning in the vulvar area were demonstrated, with dna hybridization techniques, to harbor human papillomavirus (hpv). three of the seven had intermittent, culture-negative dysuria coincident with the vulvar complaints. all the patients had mild to marked erythema of the openings of the minor vestibular glands, with exquisite tenderness on palpation with a cotton-tipped applicator. a regular histologic examination was equivocal f ... | 1988 | 2841460 |
| disseminated warts and evolving squamous cell carcinoma in a patient with acquired immunodeficiency syndrome. | a patient with acquired immunodeficiency syndrome developed widespread verrucous lesions on the hands, feet, and penis. histologic analysis revealed typical changes of verruca vulgaris and, from several sites, a transformation toward squamous cell carcinoma. southern blot analysis of deoxyribonucleic acid from penile wart tissue indicated the presence of a type of human papillomavirus that rarely is associated with carcinoma. | 1988 | 2842383 |
| search for human papillomaviruses in normal, hyperplastic, and neoplastic endometria. | twenty-eight endometria, including seven normal, five hyperplastic, and 16 neoplastic tissues, were analyzed with the southern blot hybridization technique for their possible association with human papillomaviruses (hpvs). human papillomavirus probes with types 6/11, 16, 18, and 31 failed to identify hpv dna in any of these tissues. the findings suggest that the endometrium is not a usual target for hpv infection, and that hpv is not likely to be a carcinogenic factor in the development of endom ... | 1988 | 2841629 |
| human papillomavirus--a study of male sexual partners. | male sexual partners of a cohort of women with genital-tract abnormalities which were associated with human papillomavirus infection were examined for evidence of infection with human papillomavirus. of the 214 male partners who were examined, 93.5% had visible genital lesions. of the 196 lesions that were biopsied, 72.5% showed histological evidence of infection with human papillomavirus, and only 20.4% of subjects with histological evidence of human papillomavirus were aware of a lesion. an un ... | 1988 | 2843730 |
| high-risk factors for cervical cancer. an epidemiological study in swa/namibia. | in an epidemiological study conducted in swa/namibia to document the prevalence of high-risk factors for cervical cancer 754 women belonging to a number of ethnic groups were examined. the prevalence of cervical intra-epithelial neoplasia and human papillomavirus infection was among the highest reported in the world. there was also an alarming incidence of other sexually transmitted diseases and disturbingly low usage of contraceptive methods. there is an urgent need for a comprehensive, well-or ... | 1988 | 2843990 |
| morphologic and immunohistochemical evidence of human papillomavirus capsid antigen in smokeless tobacco keratoses from juveniles and adults. | seventy-seven tissue samples from patients with either degree i, ii, or iii smokeless tobacco keratoses were examined. the tissues were evaluated for the presence of human papillomavirus (hpv) antigen by immunocytochemical staining. capsid antigen could be identified in sixteen cases (20.78%). this investigation further supports the concept that smokeless tobacco-associated leukoplakias can be correlated with hpv infection. | 1988 | 2846804 |
| epithelial alterations adjacent to 111 vulvar carcinomas. | material from 111 invasive primary vulvar carcinomas was reviewed in order to study the histopathologic changes adjacent to the neoplasia. the histopathologic characteristics of the adjacent tissue were divided into categories. dystrophic lesions were adjacent to invasive cancer in 57.6% of the cases, carcinoma in situ (cis) in 21.6% and epithelial changes suggestive of human papillomavirus infection in 18.9%. a spectrum of epithelial changes, ranging from hyperplastic dystrophy without atypia t ... | 1988 | 2841458 |
| intraepithelial and invasive neoplasia of the vulva in association with human papillomavirus infection. | colposcopic examination of 335 women with cytologically detected human papillomavirus (hpv) revealed involvement of the cervix in 316 patients (94%), vagina in 276 (82%) and vulva in 148 (44%). a symptom complex of pruritus and superficial dyspareunia was found in 98 of the 148 patients with vulvar infection (66%). histologic examination revealed hpv-associated vulvar intraepithelial neoplasia (vin) in 11 of 148 biopsies (7.4%). follow-up of the patients with hpv infection with or without vin sh ... | 1988 | 2841459 |
| [detection of human papillomavirus types 16 and 18 dna sequences in the screening of the cells related with carcinoma in situ of the uterine cervix--application of in situ hybridization for cytologic diagnosis]. | in order to investigate the relationship between the presence of human papillomavirus (hpv) dna and cervical carcinoma, we examined the cervical screening cells as well as the biopsy specimens obtained from 3 cases of severe dysplasia, 13 cases of carcinoma in situ (cis) and 2 cases of microinvasive carcinoma for the presence of hpv types 6, 11, 16 and 18 dna by dna-dna in situ hybridization using the biotinylated hpv dna probes. the results of in situ hybridization analysis revealed that hpv 16 ... | 1988 | 2844927 |
| managing women with human papillomavirus changes in cervical cytology. | of 86 women with cervical cytological evidence of human papillomavirus (hpv) infection who underwent colposcopy, 55 yielded histological confirmation of hpv infection and 17 of them had cervical intraepithelial neoplasia (cin). of 51 women followed up six months after cryotherapy of their cervical lesions, 46 showed no evidence of hpv infection in their cervices. of the patients with cin lesions, only one out of the 16 followed up had persistent cin ii six months after treatment, which gave a cl ... | 1988 | 2844652 |
| non-isotopic detection of in situ nucleic acid in cervix: an updated protocol. | recently, sensitive non-isotopic in situ hybridisation (nish) methodology for the detection of human dna and human papilloma virus (hpv) dna in archival paraffin blocks of cervix was described. an amended protocol, now used in this laboratory for detection of these genes by nish is presented. the amendments include the following: protease digestion at 37 degrees c; tissue dehydration in air rather than ethanol; stringency washing in formamide solution; blocking non-specific binding of avidin alk ... | 1988 | 2844861 |
| histologic correlates of papillomavirus infection of the vagina. | vaginal warts (condylomata) secondary to human papillomavirus (hpv) infection are most easily identified when perinuclear halos are seen with clear-cut nuclear atypia (koilocytotic atypia) in the superficial cells of the lesion. however, vaginal lesions clinically resembling condylomata may exhibit lesser degrees of nuclear atypia with findings suggestive of, but not typical for, the presence of hpv. the diagnosis and management of such lesions are unclear. we correlated histologic findings with ... | 1988 | 2845317 |
| interplay of viral and cellular proteins along the long control region of human papillomavirus type 18. | the long control region of human genital papillomavirus type 18 harbors transcription regulatory elements, such as the e6 promoter and a cell type-specific enhancer independent of the e2 protein. by performing dnase i footprint experiments in vitro with protein extracts from different cell lines and tissues we searched for cellular factors interacting with the totality of the control region. we detected a total of eight different protected sites; most of them were found with all the extracts. tw ... | 1988 | 2845145 |
| the biology and significance of human papillomavirus infections in the genital tract. | a variety of human papillomavirus (hpv) types infect the anogenital mucosa, giving rise to lesions that differ in clinical appearance, histology, and risk of malignant progression. certain high-risk types (hpvs 16, 18, 31, 33, 35 and 39) have a strong association with high-grade epithelial neoplasia and invasive carcinomas of the anogenital tract. cancer appears to have a multifactorial etiology, and hpv infection alone is probably insufficient for malignant transformation. the consistent associ ... | 1988 | 2847433 |
| expression of human papillomavirus types 16 and 18 dna sequences in cervical carcinoma cell lines. | a total of six cervical carcinoma cell lines, of which two contained human papillomavirus (hpv) type 16 and four contained hpv type 18 dna, were examined for viral rna expression by spot-blot hybridization. all six cell lines contained hpv-specific cytoplasmic poly(a)-containing rna. the same rna species were detected in both cytoplasmic and polysomal fractions, indicating nonselective translation of these messages into protein products. primer extension analysis of rna from the cell lines revea ... | 1988 | 2846777 |
| induction of anchorage-independent growth of human embryonic fibroblasts with a deletion in the short arm of chromosome 11 by human papillomavirus type 16 dna. | human embryonic fibroblasts with a large deletion (11p11.11p15.1) in the short arm of one chromosome 11 (del-11 cells) appeared to be susceptible to transformation by early human papillomavirus type 16 (hpv-16) dna, whereas diploid human embryonic fibroblasts were not. this difference in susceptibility might be explained by the absence of a tumor suppressor gene located within the deleted part on the short arm of chromosome 11. the presence of abundant viral early-gene transcripts in transformed ... | 1988 | 2846869 |
| penile human papillomavirus infection in consorts of women with genital human papillomavirus infection. a pilot study. | a study determined the incidence of human papillomavirus (hpv) on genitalia of male consorts of women with histologically proven genital hpv lesions to be 50%. | 1988 | 2847335 |
| high prevalence of genital tract papillomavirus infection in female adolescents. | to investigate clinical condyloma, abnormal cervical cytologic findings, and evidence of human papillomavirus infections, 89 adolescent girls were examined. cellular dnas extracted from exfoliated cervical cells were examined for human papillomavirus genomic sequences by southern transfer hybridization using 32p-labeled human papillomavirus dna probes. human papillomavirus sequences were detected in 12 (13%) young women, abnormal cytologic specimens in 21 (24%), and vulvar condylomas in 12 (13%) ... | 1988 | 2845351 |
| glucocorticoid-dependent oncogenic transformation by type 16 but not type 11 human papilloma virus dna. | squamous cell carcinoma of the uterine cervix is one of the most common cancers among women. correlation between human papilloma virus (hpv) infection of the uterine cervix and the development of cervical neoplasia has been established. more recent studies have shown the presence and expression of integrated hpv types 16 and 18 dna sequences in 70-80% of cervical tumours and tumour cell lines. it has been suggested that, in addition to hpvs, other agents such as hormones and tobacco products act ... | 1988 | 2847052 |
| langerhans' cells and subtypes of human papillomavirus in cervical intraepithelial neoplasia. | there is strong circumstantial evidence that human papillomavirus is a cofactor in the development of cervical neoplasia. systemic immunosuppression has also been implicated. a study was therefore carried out examining the relation between subtypes of human papillomavirus and local immunocompetent cells in the cervix. colposcopically directed punch biopsy specimens were taken from normal cervix and from histologically proved cervical intraepithelial neoplasia for immunohistochemical studies. hum ... | 1988 | 2846100 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| genital papillomavirus infection after treatment for cervical intraepithelial neoplasia (cin) iii. | genital human papillomavirus (hpv) infection was studied in 150 women after conization for cervical intraepithelial neoplasia grade three (cin iii). colposcopically directed biopsies were taken from the cervix and vulva for histopathological diagnosis. 77 specimens were further analyzed immunohistochemically for the presence of hpv capsid antigen. in ten randomly selected cervical biopsies cellular dna was dot blot hybridized with hpv 6/11 and 16/18 dna probes. genital warts were seen in 10 (7%) ... | 1988 | 2844389 |
| constitutive and steroid hormone regulated transcriptional enhancer elements in the genome of the human papilloma virus type 16, which is associated with neoplasia of the cervix. | the 7905 bp genomic dna of the human papilloma virus type 16 (hpv-16), which is associated with invasive cervical neoplasia, contains a 832 bp upstream regulatory region (urr) supposed to contain most of its cis-acting promoter elements. a search for dna sequences known to bind transcription factors has resulted in the recognition of numerous consensus sequences. a functional analysis of a segment of this urr containing some of these consensus sequences has resulted in the recognition of an enha ... | 1988 | 2846169 |
| paget's disease of the vulva: search for herpes simplex virus antigens and human papillomavirus antigen and dna. | specimens from three women with paget's disease of the vulva were tested for presence of herpes simplex virus type 2 (hsv2) antigens and human papillomavirus (hpv) antigen and dna. in one of the lesions, the hsv2-associated antigen icsp 34/35 was demonstrated. neither hpv antigens nor hpv dna were detected in the lesions. | 1988 | 2846413 |
| presence of papilloma virus type 11 in condyloma acuminatum of bladder in female renal transplant recipient. | a case is reported of a female renal transplant recipient in whom, after two years of immunosuppression, condylomata acuminata of the genital tract with urethral and bladder extension developed. the condyloma of the bladder was resected endoscopically with no relapse. virologic examination revealed a human papilloma virus type 11. | 1988 | 2845625 |