Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| expression of the human papillomavirus type 6b l2 open reading frame in escherichia coli: l2-beta-galactosidase fusion proteins and their antigenic properties. | human papillomavirus (hpv) type 6b genome contains two large open reading frames (orfs), designated l1 and l2, in a putative late region. these orfs are expected to code for viral structural proteins. to examine antigenic properties of a l2 gene product, we constructed two plasmids which contain n-terminal (l2-n) and internal (l2-i) regions of the hpv6b l2 orf and then each region was expressed in escherichia coli as a fusion protein with e. coli beta-galactosidase (beta-gal). both l2-n/beta-gal ... | 1987 | 2437699 |
| laryngeal papilloma cells in culture have an altered cytoskeleton. | we have investigated the effect of human papillomavirus (hpv) transformation on cellular cytoarchitecture. cells from laryngeal papillomas and normal epithelium were cultured in vitro. cytoskeletal components of both types of cells were visualized by immunofluorescence, to determine whether there were any differences in the structure or distribution of the cytoskeleton. there was no significant change in microtubules. two major components of the cytoskeleton, the intermediate filaments and the m ... | 1987 | 2437763 |
| structure, dnasei hypersensitivity and expression of integrated papilloma virus in the genome of hela cells. | three integrated copies of human papilloma virus-18 (hpv-18) have been identified in hela dna as hindiii bands. hpv-18 has no hindiii restriction site in its genome. the three segments: a, 8.4 kb; b, 7.9 kb and c, 5.8 kb, have an incomplete viral genome. all of them have most of the 1.1-kb bamh1 non-coding fragment of hpv-18, which seems to contain the viral origin of replication and regulatory elements. two of the segments (a and b) have a common 5'-end break-point in the viral genome within th ... | 1987 | 2439332 |
| transformation of human fibroblasts and keratinocytes with human papillomavirus type 16 dna. | human keratinocytes and fibroblasts isolated from foreskin were transformed by transfection with recombinant human papillomavirus type 16 (hpv16) dna. the transformed cells exhibited an extended (fibroblasts) or indefinite (keratinocytes) life-span compared with that of normal controls. in addition, hs27, a human fibroblast cell line previously transfected with origin-defective simian virus 40, was successfully transfected. hpv16 sequences were stably maintained in the cells, and extensive ampli ... | 1987 | 2434663 |
| multifocal squamous neoplasia of the female genital tract: significance of human papillomavirus infection of the vagina after hysterectomy. | six hundred sixteen women with a history of hysterectomy were examined for the presence of human papillomavirus deoxyribonucleic acid (dna) in vaginal smears using filter in situ hybridization. one hundred twenty patients had had hysterectomy for cervical intraepithelial neoplasia and invasive neoplasia, 54 for noncervical anogenital cancer, and 442 for benign uterine disorders. human papillomavirus dna was detected in 18% of all vaginal smears. a history of cervical neoplasia was associated wit ... | 1987 | 2819794 |
| human papillomavirus-associated lesions of the penis: colposcopy, cytology, and histology. | the diagnosis and therapy of human papillomavirus infections in men is of potential benefit because it may decrease the reservoir of virus from which genital condylomata or squamous neoplasia may arise in women. to investigate the efficacy of diagnostic methods, we examined the 127 male sexual partners of 127 women with cervical intraepithelial neoplasia. eighty-three mostly asymptomatic men (65%) had from one to 50 (median three) small (median 3 mm) human papillomavirus-associated lesions consi ... | 1987 | 2819795 |
| human papillomavirus deoxyribonucleic acid in lesions of the female genital tract: evidence for type 6/11 in squamous carcinoma of the vulva. | tissue specimens from 51 patients with genital condyloma acuminata or invasive cervical or vulvar carcinomas were analyzed for the presence of human papillomavirus deoxyribonucleic acid (dna) using the dot blot technique. of ten condylomas, 80% contained dna related to human papillomavirus 6 or 11. sixty percent had evidence of dna related to human papillomavirus 16, and 30% contained dna related to human papillomavirus 18. of 24 squamous cervical carcinomas, 58% had human papillomavirus type 16 ... | 1987 | 2819798 |
| human papillomavirus dna in squamous cell carcinomas of the larynx demonstrated by in situ dna hybridization. | a series of 116 consecutive laryngeal squamous cell carcinomas were analyzed using an in situ dna hybridization technique with 35s-labelled human papillomavirus (hpv) dna probes to demonstrate the dna of hpv types 6, 11, 16 and 30, in routinely processed, paraffin-embedded biopsies. the lesions were also assessed on light microscopy for hpv-suggestive morphological features, and by indirect immunoperoxidase (ip-pap) technique to demonstrate the expression of hpv structural proteins (viral antige ... | 1987 | 2819803 |
| cervical carcinoma as a sexually transmitted disease: human papillomavirus and its possible etiologic role. | 1987 | 2820206 | |
| evolution of human papillomavirus infections in the uterine cervix during a long-term prospective follow-up. | a series of 513 women presented with cervical human papillomavirus (hpv) infections (with or without cervical intraepithelial neoplasia; cin) have been prospectively followed up since 1981 (mean 25.6 months), at 6-month intervals, by colposcopy with pap smears and/or punch biopsies. the latter were analyzed by light microscopy, as well as immunohistochemically for hpv structural proteins. hpv dna typing was accomplished by southern blot, spot, and in situ hybridization techniques using the dna p ... | 1987 | 2820450 |
| detection of human papillomavirus-6 in primary carcinoma of the urethra in men. | the authors report a case of primary urethral carcinoma which seems to be linked to human papillomavirus (hpv) infection. southern blot hybridization of phosphorus 32 (32p)-labeled dna extracted from the cancer tissue gave a positive reaction with hpv-6. specimens of the tumor material subjected to in situ hybridization with 3h-labeled hpv-6 dna showed numerous tumor cell clusters with clearly labeled nuclei. using immunoperoxidase staining the authors found papillomavirus structural antigen wit ... | 1987 | 2820563 |
| cytologic and histologic manifestations of human papillomavirus infection of the female genital tract and their clinical significance. | the historical developments in the recognition of the role of human papillomavirus (hpv) infection in cells and tissues of the female genital tract are briefly summarized. the identification of a specific marker cell, the koilocyte, has led to initial studies of frequency and biologic significance of neoplastic lesions of the uterine cervix associated with hpv. by molecular virology techniques, over 40 types of hpv have been identified and their tissue affinity determined. types 6, 11, 16, 18, a ... | 1987 | 2820565 |
| causes and management of cervical intraepithelial neoplasia. | the evidence for human papillomavirus (hpv) playing a role in the pathogenesis of lower genital neoplasia is reviewed. it is likely that this group of viruses is etiologically important. it is suggested that cervical lesions be diagnosed as flat condyloma if they contain hpv types six or 11 and as cervical intraepithelial neoplasia (cin) if they are confined to the epithelium and contain hpv types 16 or 18 or other types associated with neoplasia. patients with a papanicolaou (pap) smear or clin ... | 1987 | 2820566 |
| detection of low copy human papilloma virus dna and mrna in routine paraffin sections of cervix by non-isotopic in situ hybridisation. | in analysing human papilloma virus (hpv) infection of the cervix in formalin fixed paraffin sections by non-isotopic in situ hybridisation two main problems were found: detachment of sections from the glass during hybridisation and probe detection; inadequate sensitivity and inability to assess sensitivity of the in situ procedure. the first problem was investigated by assessing the efficiency of various tissue adhesives individually and in combination. the second problem was addressed by optimi ... | 1987 | 2821078 |
| demonstration of human papillomavirus dna in oral condyloma acuminatum. | condyloma acuminatum arising on oral mucous membranes exhibits distinct histopathological features that allow for differentiation from the more common squamous papilloma. these lesions are parakeratinized with cryptic invagination of cornified cells extending into an acanthotic spinous cell layer. superficial spinous layer koilocytosis is a prominent feature and the condylomatous papillomas exhibit a tendency for sessile architecture. among 62 instances, the average age was 39.7 years with a pre ... | 1987 | 2821213 |
| human papillomavirus standardization and dna cytophotometry in cervical intraepithelial neoplasia. | human papillomavirus (hpv) standardization and dna cytophotometry were carried out in 29 cases of cervical intraepithelial neoplasia (cin) grades i-iii. a prognostically unfavorable dna distribution pattern with an aneuploid stemline was found in 14 of the 16 dysplasias with hpv types 16 and 18, while in 11 of 13 dysplasias with hpv types 6 and 11 there was a favorable dna distribution with a euploid-polyploid stemline. among 178 colposcopically, cytologically and histologically confirmed cervic ... | 1987 | 2821252 |
| oncogenic association of specific human papillomavirus types with cervical neoplasia. | molecular hybridization analysis of human papillomavirus (hpv) dna from 190 cervical biopsy specimens from women in the united states, brazil, and peru revealed viral sequences in 2 (9%) of 23 biopsy specimens of normal mature squamous epithelium, 7 (44%) of 16 biopsy specimens of metaplastic squamous epithelia, 60 (77%) of 78 cervical intraepithelial neoplasia (cin), 57 (89%) of 64 invasive squamous carcinomas, and 8 (89%) of 9 endocervical adenocarcinomas. hpv typing by dna hybridization revea ... | 1987 | 2821311 |
| chromosomal integration sites of human papillomavirus dna in three cervical cancer cell lines mapped by in situ hybridization. | metaphase chromosomes of three cervical cancer cell lines (hela, caski, siha) were subjected to in situ hybridizations with the dna of human papillomaviruses (hpv) types 16 and 18, respectively. previous studies have demonstrated multiple copies of hpv 18 dna in hela and of hpv 16 dna in caski cells, but only 1-2 hpv 16 copies in cells of the siha line. the viral dna persists in an integrated state (schwarz et al 1985). analysis of the integration sites revealed at least 11 chromosomal sites of ... | 1987 | 2821369 |
| the morphology of butchers' warts as related to papillomavirus types. | hand warts were studied in 160 butchers. clinical and histological studies were performed in 190 warts and virological studies in 165 warts from 104 butchers. since we found almost perfect correlation between the histological pattern and the type of infecting virus, it was possible to evaluate the virus types in a further 39 of 56 butchers without virological studies, on the basis of the histology of the warts. the most common infection was with hpv-2 (human papilloma virus) and hpv-7. thirty-th ... | 1987 | 2821930 |
| detection of human papillomavirus dna in biopsies of human oral tissue. | we have employed molecular probes produced from dna fragments of human papillomavirus, cloned into prokaryotic vectors, to detect virus nucleic acid sequences in extracts of human oral tissues. the study was conducted with duplicate coded snap-frozen tissue biopsies from which frozen sections had been taken to accurately assess the pathology of each particular sample. the results show that a large proportion of the oral biopsies contained dna which hybridized to the viral dna probes, even under ... | 1987 | 2822070 |
| natural killer cells in cervical intraepithelial neoplasia and human papillomavirus infection. | a semiquantitative immunocytochemical study of natural killer (nk) cells in cervical mucosa was performed by the immunoperoxidase technique using anti-leu-7 and anti-leu-11 monoclonal antibodies. nk cells were present in two of the five normal controls and five of six specimens showing human papillomavirus (hpv) infection. of the 12 cin studied, six were positive for both leu-7 and leu-11 staining and three were positive for leu-11 alone. nk cells were found predominantly in the subepithelial st ... | 1987 | 2822078 |
| [histological aspects of lesions of the cervix uteri correlated with different types of human papillomavirus]. | cervical biopsies from 66 women presenting an abnormal smear, with hpv related features were studied and histologic features were correlated to the hpv type, as determined by molecular hybridisation studies. hpv dna sequences were evidenced in 13 of 19 lesions corresponding to exophytic or flat condyloma (hpv type 16 in seven cases, hpv type 11 in four cases, as yet uncharacterized hpv types, hpv x, in two cases). fourty biopsies were histologically interpreted as cin, on the basis of atypical m ... | 1987 | 2822182 |
| sensitivity of the cytologic diagnosis of cervical condyloma in comparison with hpv-dna hybridization studies. | the cytologic diagnosis of cervical condyloma is based on criteria developed over the last 10 years. it has now become possible to document the presence of human papilloma virus (hpv) dna directly in cervical swabs by the highly sensitive technique of dna filter hybridization in situ. the purpose of this article is to evaluate critically the empirically established cytologic criteria of condyloma by comparing them with hpv-dna hybridization studies in the same material. the results of this study ... | 1987 | 2822367 |
| case-control study of human papillomaviruses and cervical cancer in latin america. | human papillomavirus (hpv) types 16 and 18 have been implicated as risk factors for cervical dysplasia and neoplasia. however, most studies have been observational, uncontrolled and conducted in populations at low risk for invasive cancer. we report a pilot case-control study of incident invasive cervical cancer in panama, costa rica and bogota, colombia. between july and september 1985 we enrolled 46 consecutive newly diagnosed invasive cervical cancer cases and 51 age-matched control women. su ... | 1987 | 2822583 |
| in situ localization of human papilloma virus type 16 dna in a metastasis of an endocervical adenocarcinoma. | metastatic tissue from an endocervical adenocarcinoma was examined for human papilloma virus (hpv) dna sequences. southern blot analysis with 32p-labeled viral probes revealed the presence of about 150 copies of hpv-16 dna per cellular genome. dna in situ hybridization of frozen sections with biotinylated probes showed that hpv-16 dna was exclusively detectable in the nuclei of the malignant epithelial cells and not in the stroma. | 1987 | 2822599 |
| herpes simplex virus and human cytomegalovirus: their role in morphological transformation and genital cancers. | herpes simplex virus (hsv) and human cytomegalovirus (hcmv) are candidates for the induction of premalignant or malignant disease. morphological transformation studies have failed to demonstrate a viral oncogene, a virus-coded transforming protein or any sequence of dna that uniquely transforms cells according to one-hit kinetics. thus the mechanism of transformation is complex. the transformed cells are, however, all oncogenic in the host animal and in immunocompetent mice. direct evidence for ... | 1987 | 2822840 |
| human papillomavirus (hpv) in oral squamous cell papillomas. | one hundred and forty oral squamous cell papillomas (scps) were subjected to immunocytochemical staining for papillomavirus antigen using the immunoperoxidase-pap method 41% of the oral squamous papillomas showed positive intranuclear staining, 50% were negative and the remaining 9% showed both intranuclear and cytoplasmic staining. in view of these findings it is suggested that a review of the etiology of lesions diagnosed as oral scps should be considered. | 1987 | 2822883 |
| the e6-e7 region of human papillomavirus type 18 is sufficient for transformation of nih 3t3 and rat-1 cells. | plasmids containing the e6 and e7 open reading frames of human papillomavirus type 18 expressed from an autologous transcriptional control region were sufficient for transformation of nih 3t3 and rat-1 cells. transformation by these sequences did not always involve morphological alterations even though anchorage-independent growth occurred at a high frequency. in these cells, the efficiency of transformation by the e6 and e7 construct was equivalent to or, in most cases, better than that observe ... | 1987 | 2822969 |
| hpv dna detection in tumours of the head and neck: a comparative light microscopy and dna hybridization study. | a series of 22 squamous cell carcinomas (4 cases grade 1; 11 cases grade 2; 7 cases grade 3) of the oral cavity (13 cases), (naso-)pharynx (5 cases) and larynx (4 cases) were studied by conventional light microscopy and filter (dot blot) hybridization for the detection of human papillomavirus (hpv) dna. in 4 carcinomas, tumour-free tissue from the resection margins of the surgical sample was examined in addition to the tumour specimen. the same kind of investigation was carried out on 4 oral leu ... | 1987 | 2823203 |
| extraction of dna from formalin-fixed paraffin-embedded pathology specimens and its use in hybridization (histo-blot) assays. application to the detection of human papillomavirus dna. | 1987 | 2823232 | |
| [human papillomavirus infections of the cervix uteri. i. pathobiology, epidemiology and relation to cancer pathogenesis]. | infection of the uterine cervix by human papilloma viruses (hpv) is a ubiquitous yet only recently recognized lesion. the morphological findings correlate with early and late gene expression. the pathological events presumably depend upon the hpv type involved and upon environmental and host factors. the recognized risk factors are those described for epithelial dysplasias and carcinomas. the prevalence is unclear: it varies among different population groups and depends upon the investigation me ... | 1987 | 2823379 |
| human papillomavirus and the squamous epithelium of the female genital tract. | the evidence linking human papillomavirus (hpv) infection with cervical intra-epithelial neoplasia (cin), cervical cancer and similar changes in the vulval and vaginal epithelium is reviewed. reference is made to prevalence rates for hpv and cin in south africa and south west africa/namibia and the prospect of an 'epidemic' of cervical and possibly of other squamous genital cancers is discussed. only an effective cytological screening programme with effective follow-up arrangements will prevent ... | 1987 | 2823398 |
| integration of human papillomavirus type 16 into cellular dna of cervical carcinoma: preferential deletion of the e2 gene and invariable retention of the long control region and the e6/e7 open reading frames. | the integration patterns of human papillomavirus (hpv) type 16 in the cellular dna of six cervical carcinoma samples were analyzed by the southern blot procedure. none of the hpv integrants retained the entire viral genome. double hpv integration was found in one case while all other cases were single integrants. in some samples, internal deletion and selective amplification of the viral sequences were observed. on integration, the e2 open reading frame (orf) was invariably lost but the e6/e7 or ... | 1987 | 2823467 |
| detection of human papillomavirus (hpv) structural antigens and dna types in inverted papillomas and squamous cell carcinomas of the nasal cavities and paranasal sinuses. | to assess the suggested etiological role of human papillomavirus (hpv), biopsies from 14 patients operated on for an inverted papilloma (11 cases) and squamous cell carcinoma (3 cases) of the nasal cavity and paranasal sinuses were analysed for light microscopical evidence of hpv, by indirect immunoperoxidase (ip-pap) to demonstrate hpv structural proteins, and using in situ dna-hybridization to disclose the dna of hpv types 6, 11 and 16. the majority of the inverted papillomas contained areas o ... | 1987 | 2823523 |
| colposcopic correlates of cervical papillomavirus infection. | we compared the colposcopic and histologic features of 39 papillomavirus-related cervical lesions with their associated human papillomavirus type. two thirds of the lesions contained human papillomavirus type 16-related deoxyribonucleic acid, and 69% of them were classified as cervical intraepithelial neoplasia i or ii, in contrast to 92% of lesions containing human papillomavirus 6/11 deoxyribonucleic acid. on histologic examination, low-grade cervical intraepithelial neoplastic lesions tended ... | 1987 | 2823611 |
| epidermodysplasia verruciformis. a case associated with primary lymphatic dysplasia, depressed cell-mediated immunity, and bowen's disease containing human papillomavirus 16 dna. | epidermodysplasia verruciformis is a rare, often hereditary disease characterized by a generalized cutaneous infection with human papillomavirus (hpv), depressed cell-mediated immunity, and a propensity for transformation of the warty lesions to squamous cell carcinoma on primarily sun-exposed areas of the skin. a 37-year-old man with congenital lymphatic dysplasia and a history of squamous cell carcinoma of the groin and foot was observed by us to have edema of all four extremities, numerous fl ... | 1987 | 2823726 |
| bowen's disease of the feet. presence of human papillomavirus 16 dna in tumor tissue. | a 36-year-old black man with bilateral squamous cell carcinoma in situ of the feet is described. the dna hybridization analysis performed on the tumor tissue demonstrated the presence of human papillomavirus 16 dna. human papillomavirus 16 has been detected repeatedly in genital carcinomas, and evidence is mounting that it may play an important role in the development of malignancy. | 1987 | 2823727 |
| cervical human papilloma virus infection and colposcopy. | 1987 | 2823782 | |
| human papilloma virus infection and associated intraepithelial neoplasia of the cervix, vagina and vulva. | a prospective colposcopic and histological study was performed on 333 patients with cytologically detected human papilloma virus infection (hpv) without cytological evidence of associated intraepithelial neoplasia. the colposcopic changes seen in the cervix, vagina and vulva were documented and target biopsies were taken from abnormal areas in these 3 sites. colposcopic and histological evidence of hpv infection was found in the cervix of 314 patients (94%), vagina in 274 patients (82%) and vulv ... | 1987 | 2823783 |
| the significance of human papilloma virus atypia ('wart virus infection') found alone on cervical cytology screening. | a series of 429 patients has been analysed in which the cytology smear report suggested human papillomavirus (hpv) infection alone. all patients were examined colposcopically and in 58 (13.6%) associated histologically confirmed cervical intraepithelial neoplasia (cin) was demonstrated. cin subsequently developed in an additional 10% of those patients who returned for follow-up over an ensuing 3-year period. follow-up with repeat cervical cytology smears alone was unreliable as 4% of patients ev ... | 1987 | 2823784 |
| [human papillomavirus infection and carcinogenesis of cancer of the uterine cervix. i. pathomorphology of 357 cervical tissues]. | human papillomavirus (hpv) infection in the cervix was studied by pathomorphology for the first time in china according to meisels and fortin's criteria. cervical condyloma was found in 257 out of 357 (71.99%) cervical tissues. 141 were simple condyloma, 50 dysplasia with condyloma and 66 carcinoma with condyloma. a zone of continuous transformation was observed between the condyloma and dysplasia or carcinoma. 141 simple condylomas were initially diagnosed as chronic cervicitis in 94 (66.67%) a ... | 1987 | 2824150 |
| detection of human papillomavirus type 6/11 dna in conjunctival papillomas by in situ hybridization with radioactive probes. | twenty-three conjunctival papillomas and 28 conjunctival dysplasias were examined for human papillomavirus (hpv)-dna sequences by in situ hybridization with nick-translated 35s-labeled hpv probes. adjacent paraffin sections were hybridized with hpv type 2, 6, 16, and 18 probes at tm - 17 degrees c. fifteen tissues, all papillomas, displayed positive hybridization with the hpv-6 probe. infection with hpv-6 (or the closely related hpv-11) appeared to be responsible for most of the conjunctival pap ... | 1987 | 2824322 |
| chromosomal insertion of human papillomavirus 18 sequences in hela cells detected by nonisotopic in situ hybridization and reflection contrast microscopy. | genomic insertion of human papillomavirus (hpv) sequences is associated with the genesis of cervical carcinoma, and hpv-induced incipient cellular alterations may also present a requisite for the establishment of cell lines such as hela. considering the theoretical importance of specific viral integration sites, we attempted to detect in hela cells the chromosomal location of dna sequences homologous to hpv-16 and hpv-18 sequences by a nonisotopic high resolution in situ hybridization technique. ... | 1987 | 2824333 |
| new human papillomavirus sequences in female genital tumors from japanese patients. | tissues from 52 cervical carcinomas, 15 cervical intraepithelial neoplasias iii (cins iii), and 3 vulvar carcinomas were examined for the presence of human papillomavirus (hpv) sequences by southern blot hybridization with hpv 16 or 18 dna as the probe. hpv 16 or 18 dna was detected in only 17 cervical carcinomas (33%), 5 cins iii (33%), and 1 vulvar carcinoma (33%). these frequencies are lower than those reported by others. however, new, as yet unidentified, hpvs were also detected at rather hi ... | 1987 | 2824417 |
| human papillomaviruses in oral epithelial lesions. comparative study between histopathology and immunohistochemistry in routine diagnosis. | forty five oral papillary lesions classified clinically and histologically as condylomatous papilloma, hyperkeratotic papilloma and oral wart were subjected to histopathological evaluation and tested with human papilloma virus (hpv) antiserum, in order to detect hpv group-specific antigens. the immunoperoxidase labelling technique was positive in 10% of 31 condylomatous papillomas, 22% of 9 hyperkeratotic papillomas, and 60% of 5 oral warts. no viral antigens could be detected in 4 oral fibromas ... | 1987 | 2824453 |
| routine papillomavirus antigen staining of cervical punch biopsy specimens. | immunocytochemical staining for papillomavirus antigen was carried out on 1147 consecutive cervical punch biopsy specimens over 12 months. of 876 cases with cervical intraepithelial neoplasia (cin) 351, were antigen positive and of 49 cases with histological evidence of human papillomavirus (hpv) infection but no cin, 14 were positive. there were 204 cases reported to be normal on routine histological examination and 12 cases reported to show features suggestive but not diagnostic of hpv infecti ... | 1987 | 2824568 |
| symptomatic and asymptomatic cervical infections with human papillomavirus during pregnancy. | the prevalence of human papillomavirus (hpv) infection of the cervix was determined in an unselected population of pregnant women presenting to an inner-city obstetrics clinic in the first trimester. cervical scrape specimens were screened for the presence of hpv types 6, 11, 16, 18, and 31 dna by using three different blot hybridization methods. specimens from 26 (11.1%) of 234 patients contained hpv dna sequences. hpv-16 and -31 were detected in six specimens each, whereas hpv-6, -11, and -18 ... | 1987 | 2824625 |
| a one-step method for detecting and typing human papillomavirus dna in cervical scrape specimens from women with cervical dysplasia. | we studied 66 women with a previous dysplastic cervical cytological smear who were referred for colposcopy and biopsy for the presence of human papillomavirus (hpv) types 6, 11, 16, 18, and 31 dna in cervical specimens. the specimens were analyzed by a novel hybridization method termed reverse blotting, in which cellular dna is radiolabeled and used to probe a battery of cloned hpv dnas immobilized on nitrocellulose. reconstruction experiments demonstrated that this method could detect about one ... | 1987 | 2824626 |
| identification of human papillomavirus type 16 e7 protein by monoclonal antibodies. | a number of human papillomavirus (hpv) type 16 proteins have recently been identified in human cervical carcinoma cell lines using polyclonal antisera against papillomavirus gene products expressed in escherichia coli. e7 protein has been found to be the most abundant papillomavirus protein in these cells. here we describe a panel of monoclonal antibodies recognizing a 15k mr non-glycosylated cytoplasmic hpv-16 e7 protein. one of the antibodies cross-reacted with hpv-18 e7 protein. | 1987 | 2824670 |
| human cytomegalovirus (hcmv) enhances bovine papilloma virus (bpv) transformation in vitro. | infection of nih 3t3 cells with a combination of hcmv and bpv resulted in more foci than infection with bpv alone. foci were microscopically apparent at 4 days in the mixed infection and did not appear until 2 days later in the cultures infected with bpv alone. the enhancement was abolished by heat inactivation of the hcmv and also when the hcmv was replaced by a "mock inoculum." southern blot analysis of cellular dna from transformed cells showed a similar amount of extrachromosomal bpv dna in ... | 1987 | 2824682 |
| demonstration of human papillomavirus types 6 and 11 in juvenile laryngeal papillomatosis by in-situ dna hybridization. | a study is reported in which an in situ hybridization technique for the demonstration of human papillomavirus (hpv) employing a biotin--streptavidin polyalkaline phosphatase complex has been successfully applied to formalin-fixed paraffin-processed tissue obtained from ten patients with juvenile laryngeal papillomatosis. in all cases, positive results were obtained for either hpv type 6 or 11. normal vocal cord epithelium was negative. | 1987 | 2828588 |
| cervical and vaginal pathology in women with vulvar condylomata. | all women referred to the department of obstetrics and gynecology, school of medicine, state university of new york at stony brook, for evaluation of vulvar condylomata were reviewed to identify concurrent cervical and vaginal pathology. over the five-year period 1981-1985, referrals to our colposcopy clinic for vulvar condylomata increased from 7.8% to 29.2%. all patients underwent a thorough history, physical examination and colposcopic evaluation of the cervix, vulva and vagina. of the patien ... | 1987 | 2828612 |
| [gene molecular and ultrastructural studies on the relationship between human squamous epithelial carcinoma of the uterine cervix and the human papilloma virus]. | 1987 | 2832086 | |
| natural history of human papillomavirus infection of the anogenital tract. | a number of viruses, most notably herpes virus type 2, have been suggested as etiological agents of cervical neoplasia. recent studies with human papillomaviruses, however, have demonstrated a remarkable association of a subgroup of these viruses with about 90% of benign, preinvasive and invasive lesions of the cervix and anogenital tract. the oncogenic potential of papillomaviruses has been demonstrated both in laboratory animals and in cultured cells. furthermore, susceptibility to certain hum ... | 1987 | 2832090 |
| a study on the relationship between human papillomavirus infection and cervical carcinoma development. immunoperoxidase localization of papillomavirus antigen in cervical tissue. | 1987 | 2832133 | |
| transcription of human papillomavirus type-18 dna in human cervical carcinoma cell lines. | 1987 | 2832273 | |
| human papillomavirus type 2 dna in oral and labial verruca vulgaris. | twenty instances of verruca vulgaris, equally divided between oral mucosa and lip vermillion, were assayed for the presence of human papillomavirus (hpv) capsid antigen and type-specific dna employing in situ hybridization methods. all 10 cases arising on lip vermillon expressed capsid antigen and harbored hpv type 2 genomes as assessed under conditions of high stringency dna hybridization. oral verrucae rarely expressed capsid antigen; hpv type 2 genomes were encountered in 20% of the cases. | 1987 | 2832456 |
| a method of in situ molecular hybridization applied to the study of viral papillomas in man. | thirty nine papillomas and 14 precancerous lesions, from skin and mucosa were studied for the presence of human papillomavirus (hpv) infection on frozen sections or on paraffin embedded sections by comparison of 2 methods: (1) detection of group specific viral antigen by immunohistochemical techniques with a rabbit antiserum raised to highly purified virus dissociated by sds (sodium dodecylsulfate) and heating: (2) detection of viral dna by an in situ molecular hybridization technique with bioti ... | 1987 | 2833145 |
| relationship between human papillomavirus infection and uterine cervical carcinoma development. a pathomorphological observation of 357 cases. | 1987 | 2834145 | |
| venereal papilloma and papillomavirus in a colobus monkey (colobus guereza). | a papilloma on the penis of a colobus monkey was found to contain papilloma-virus group specific antigens by immunohistochemical analysis and virus-like particles in the nuclei of epithelial cells by transmission electron microscopy. in low-stringency southern blot hybridizations, dna from the lesion annealed with human papillomavirus 11 dna, but not with the dnas of 13 other papillomaviruses. using human papillomavirus 11 dna as a probe in southern blot hybridizations, dna from the penile papil ... | 1987 | 2835331 |
| increased incidence of cervical intraepithelial neoplasia grade 3 and human papillomavirus infection at a family planning clinic. | a five-fold increase in the incidence of histologically-proven cervical intraepithelial neoplasia grade 3 (cin3) was observed for patients attending the christchurch family planning clinic in 1985 compared with the preceding three years. there were 51 new cases in 1985 and a yearly average of 9.3 between 1982 and 1984. there was an increase also in cervical smears showing evidence of human papillomavirus (hpv) infection. the time interval between the last normal smear and the diagnosis of cin3 w ... | 1987 | 2837702 |
| [human papillomavirus infection and carcinogenesis of cancer of uterine cervix. ii. immunoperoxidase localization of papilloma-virus antigen in tissues of the uterine cervix]. | 244 cervical tissue specimens with different diagnoses were stained by peroxidase-anti-peroxidase technique (pap) for assay of the human papillomavirus (hpv) genus-specific antigen, the first of its kind performed in china. hpv antigen was detected in 41.93% of simple condyloma group, in 35.29% of dysplasia with condyloma and in 27.27% of carcinoma with condyloma. there was no positive in the control, dysplasia or carcinoma groups. the antigen positive nuclei were found in the koilocytes or para ... | 1987 | 2838240 |
| [relation between human papillomavirus (hpv) and cancer of uterine cervix]. | hpv-dna fragments were detected in biopsy specimens (29 cases of cancer of uterine cervix, 2 cervical dysplasia and 9 normal cervix) using dna hybridization technique. it was demonstrated that 52% of biopsy specimens of the cancer of uterine cervix was positive for hpv 16 dna probe, while 9% was positive for hpv 18 dna probe. 11% of non-cancerous biopsy specimens had a positive result for hpv 16 dna probe. it was also demonstrated that the positive rate of hpv 16 dna was 75% in grossly cauliflow ... | 1987 | 2838241 |
| the natural history of human papillomavirus (hpv) atypia of the cervix. | this study examines the natural history of human papilloma virus (hpv) atypia of the cervix in 259 untreated patients who were followed for 3 to 74 months (median 18 months). progression to cervical intraepithelial neoplasia (cin) occurred in 41 patients (15.8%)--all but 3 progressions occurred within the first 24 months of follow-up. in no patient did invasive cancer develop during this study. persistence occurred in 39.4% and regression in 44.8% patients. an increasing number of regressions we ... | 1987 | 2840059 |
| multifocal invasive carcinoma of the vulva in a 25-year-old woman with bowenoid papulosis. | this case report documents malignant progression associated with wart virus infection of the vulva in a 25-year-old female. the initial condition of bowenoid papulosis and carcinoma in situ of the vulva was diagnosed on colposcopic biopsies performed to investigate chronic pruritus and superficial dyspareunia. this condition failed to resolve with local ablative therapy and progressed over a period of 8 months to multifocal invasive carcinoma of the vulva requiring radical surgery. deoxyribonucl ... | 1987 | 2840060 |
| genital human papillomavirus infections. | in summary, the development of new techniques to identify hpv dna in genital secretions and tissue, the recognition of subclinical hpv infection, and the remarkable association between hpv and genital neoplasia have markedly increased the concern of both patients and physicians about genital wart virus infections. the prevalence of this viral std appears to be increasing and the clinical spectrum of disease appears to be expanding. new methods to diagnose genital hpv infection and techniques to ... | 1987 | 2848068 |
| molecular and cytogenetic analysis of immortalized human primary keratinocytes obtained after transfection with human papillomavirus type 16 dna. | a proliferating population of human foreskin keratinocytes (presently in the sixtieth passage) has been obtained after transfection with human papillomavirus (hpv) type 16 dna. in contrast, the control cultures did not survive beyond the sixth passage. cytogenetic analysis of cells taken from the twelfth passage revealed a heteroploid male karyotype. in approximately 50% of the cells a common marker chromosome was found, suggesting a clonal origin for at least part of the population. this is fur ... | 1987 | 2838778 |
| [cytology in human papilloma virus (hpv) infections: is it possible to fix cytologic standards to determine the timing of activation?]. | 1987 | 2851547 | |
| human papillomavirus dna in bronchial squamous cell carcinomas. | 1987 | 2880009 | |
| human papillomavirus dna and oral mucosa. | 1987 | 2880158 | |
| prognosis of women with human papillomavirus dna in normal tissue distal to invasive cervical and vulval cancer. | 1987 | 2881111 | |
| human papillomavirus and cervical cancer. | 1987 | 2882384 | |
| identification and mapping of human papillomavirus type 1 rna transcripts recovered from plantar warts and infected epithelial cell cultures. | multiple spliced transcripts of human papillomavirus type 1 were detected by electron microscopic analysis of r-loops formed with total rna extracted from plantar warts and with poly(a)+ rna isolated from cultured keratinocytes infected with human papillomavirus type 1. the 5' ends of the rnas were mapped to sites in the e7 open reading frame (orf), just upstream of the e6 orf and in the upstream regulatory region. species with 5' ends in e7 accounted for over 95% of all transcripts seen. two po ... | 1987 | 2883327 |
| human papillomavirus type 16 dna in anal squamous cell carcinoma. | 1987 | 2885526 | |
| human papillomavirus type 5 and skin cancer in renal allograft recipients. | 1987 | 2885608 | |
| a human papilloma virus type 11 transcript encoding an e1--e4 protein. | the human papilloma virus (hpv) associated with a genital wart (condyloma acuminatum) was determined to be type 11. the majority of the viral dna molecules were monomeric circles present in the cells at high copy number, as demonstrated by one- and two-dimensional agarose gell electrophoretic separation followed by southern blot analysis. a cdna library in phage lambda gt11 was constructed from poly(a)-selected mrna recovered from the tissue. recombinant clones corresponding to the most abundant ... | 1987 | 2887066 |
| human papillomavirus frequency in normal cervical tissue. | 1987 | 2887806 | |
| human papillomavirus infections in women with and without abnormal cervical cytology. | 9295 smears, obtained from women attending three gynaecological hospitals for routine screening, were examined for human papillomavirus (hpv) types 6 and 11 and hpv 16 and 18 infections by filter in-situ hybridisation. the data were compared with cytological findings. in women with normal cytological smears hpv infection was identified in about 10% of women aged between 15 and 50 years and in less than 5% of those aged over 50. in women with abnormal smears (cervical intraepithelial neoplasia [c ... | 1987 | 2888940 |
| [a comparative study of sensitivities of immunocytochemistry, koilocytosis and transmission electron microscopy in detecting hpv in the cervical condyloma and intraepithelial neoplasia as compared to dna hybridization]. | the sensitivity in detection of human papillomavirus (hpv) by immunocytochemistry, histological observation of koilocytosis and electron microscopy with reference to the results of southern blot dna hybridization were reviewed in 41 lesions (37 patients) of cervical and vaginal condylomata acuminata and intraepithelial neoplasia. hpv dna was demonstrated in all but one lesion of moderate dysplasia (98%). hpv capsid antigens were demonstrated by immunocytochemistry in approximately 60% of the les ... | 1987 | 2828491 |
| human papillomavirus (hpv) and its role in oral disease--an overview. | 1987 | 2852565 | |
| expression of the human papillomavirus type 18 e7 gene by a cassette-vector system for the transcription and translation of open reading frames in eukaryotic cells. | we have constructed and functionally tested a cassette-vector-system for the transcription and translation of open reading frames (orfs) in cells of higher eukaryotes. the vectors are derived from the plasmid pbr322 and can be selected and amplified in escherichia coli. alternative eukaryotic promoters can be inserted between the restriction sites sphi and kpni, translation initiation motifs between kpni and bglii, linkers for the adjustment of the translation reading frame and the insertion of ... | 1987 | 3034570 |
| identification of early proteins of the human papilloma viruses type 16 (hpv 16) and type 18 (hpv 18) in cervical carcinoma cells. | we have sequenced 1730 bp of human papilloma virus type 18 (hpv 18) dna containing the open reading frames (orf) e6, e7, the n-terminal part of e1 and, additionally, 120 bp of the n-terminal part of l1. based on these sequencing data, together with the human papilloma virus type 16 (hpv 16) dna sequence published recently, we identified and cloned the orf e6, e7, e1 and l1 of hpv 18 and the orf e6, e7, e1, e4, e5, l2 and l1 of hpv 16 into prokaryotic expression vectors. the expression system use ... | 1987 | 3034571 |
| [pathological changes in the cervix uteri in human papillomavirus infection (hpv)]. | 1987 | 3034743 | |
| distribution pattern of human papilloma virus 16 genome in cervical neoplasia by molecular in situ hybridization of tissue sections. | using a highly sensitive method with single-stranded rna probes, we analyzed the distribution pattern of hpv 16 dna by in situ hybridization in cin ii (10 cases), cin iii (11 cases) and in invasive cervical carcinoma (17 cases). the technique used detected as little as 20-50 viral genomes per cell. this sensitive technique unmasked hpv 16 genomes in the basal cells of all forms of cin. in cin iii viral genomes were present throughout the entire thickness of the epithelium. there was a striking d ... | 1987 | 3034804 |
| detection of specific types of human papillomavirus in cervical scrapes, anal scrapes, and anogenital biopsies by dna hybridization. | specific varieties of human papillomavirus (hpv) infecting the anogenital region were detected in clinical samples by use of a filter hybridization technique suitable for rapid screening of cervical and anal scrapes. in this way possibly benign types (hpv6 and hpv11) could be differentiated from types thought to be capable of malignant transformation (hpv 16 and hpv 18). cervical or anal canal cells were applied directly to nylon filters and fixed by u.v. irradiation before hybridization with mi ... | 1987 | 3035075 |
| laryngeal papillomatosis: clinical, histopathologic and molecular studies. | the clinical course and pathology of 57 patients with laryngeal papillomatosis were reviewed. tissues from 26 patients were analyzed for human papillomavirus (hpv) dna by southern blot hybridization. histopathologic evaluation of the papillomas showed no correlation with age of onset or clinical pattern of remission and recurrence. the pathology was characterized by abnormal squamous maturation with parakeratosis, retardation of superficial cell maturation, papillomatosis, and basal hyperplasia. ... | 1987 | 3035299 |
| assessment of precancerous lesions of the uterine cervix for evidence of human papillomavirus infection: a histological and immunohistochemical study. | cervical biopsies obtained by colposcopic direction from 358 women were histologically examined for squamous dysplasia (cervical intra-epithelial neoplasia; cin) and human papillomavirus (hpv) infection. of the 358 biopsies, 136 were stained by an immunoperoxidase method using an antiserum against genus-specific (common) antigen of bovine papillomavirus. hpv antigens were detected in 40% of biopsies showing definite histological evidence of hpv effect, and in 7.9% and 2.6% of those with possible ... | 1987 | 3035470 |
| human papillomavirus-associated early vulvar neoplasia investigated by in situ hybridization. | of 21 consecutive cases of early vulvar neoplasia studied at the istituto nazionale tumori of milan, 62% appeared to be related to papillomavirus infection. this conclusion is the result of the present study by in situ hybridization with dna probes of human papillomavirus (hpv) 6/11, 16, and 18 and of previous ultrastructural and immunohistochemical investigations. the proportion of cases associated with hpv was 78.5% for those (11/14) with histologic evidence of viral infection and 33% for thos ... | 1987 | 3035953 |
| identification of the hpv-16 e6 protein from transformed mouse cells and human cervical carcinoma cell lines. | human cervical carcinoma cell lines that harbor human papillomavirus (hpv) have been reported to retain selectively and express hpv sequences which could encode viral e6 and e7 proteins. the potential importance of hpv e6 to tumors is suggested further by the observation that bovine papillomavirus (bpv) e6 can induce morphologic transformation of mouse cells in vitro. to identify hpv e6 protein, a polypeptide encoded by hpv-16 e6 was produced in a bacterial expression vector and used to raise an ... | 1987 | 3036495 |
| purification of papillomavirus structural polypeptides from papillomas by immunoaffinity chromatography. | a broadly cross-reactive monoclonal antibody directed against papillomavirus, coupled to immunoaffinity columns, was used to isolate bovine papillomavirus type 1 (bpv-1) and human papillomavirus type 1 (hpv-1) structural polypeptides from homogenates of productively infected cells. one of the polypeptides isolated from bovine fibropapillomas appeared to be the bpv-1 major capsid protein since it had a mol. wt. of 54k and was reactive by western blots with papillomavirus genus- and bpv-1 type-spe ... | 1987 | 3037012 |
| expression of human papillomavirus types 6b and 16 l1 open reading frames in escherichia coli: detection of a 56,000-dalton polypeptide containing genus-specific (common) antigens. | the human papillomavirus (hpv) genome contains two large open reading frames (orfs), designated l1 and l2. to characterize the antigenic properties of the l1 orf-encoded proteins, we cloned the l1 orfs of hpv6b and hpv16 in plasmids, and these were expressed in escherichia coli. first, the hpv6b dna, representing 85.2% of the l1 orf, was cloned in puc19 and expressed in e. coli jm83 and rb791 as a 160,000-molecular-weight (160k) fusion protein with e. coli beta-galactosidase (6bl1/beta-gal). sec ... | 1987 | 3037103 |
| human papillomavirus types 6 and 11 mrnas from genital condylomata acuminata. | we have identified and mapped a number of rna species of human papillomavirus types 6 and 11 from condylomata acuminata by the electron microscopic r-loop technique. each of the early (e)- and late (l)-region open reading frames (orfs) deduced from the dna sequences was represented in one or more transcripts. in addition, rna species that could encode the modulator of dna replication and the repressor of transcription, functions recently identified in the genetically similar bovine papillomaviru ... | 1987 | 3037118 |
| enhancers and trans-acting e2 transcriptional factors of papillomaviruses. | the upstream regulatory regions of human papillomavirus (hpv) types 1, 6b, 7, 11, 16, and 18, bovine papillomavirus type 1, and cottontail rabbit papillomavirus were cloned into transcriptional enhancer assay plasmids which carry the simian virus 40 early promoter lacking its own enhancer and the bacterial gene encoding chloramphenicol acetyltransferase (ec 2.3.1.28) (cat). enhancer activity, reflected by cat gene expression, was detected in all of the upstream regulatory regions tested only whe ... | 1987 | 3037119 |
| characterization of human papilloma virus types in condylomata acuminata in children by in situ hybridization. | the presence of human papilloma virus (hpv) dna sequences of types 6, 11, 16, and 18 was determined by in situ hybridization under stringent conditions on archival paraffin-embedded tissue sections in eight condylomata acuminata observed in children below the age of 12 years. viral sequences were detected in seven of eight cases: all of them contained hpv 6, four contained also hpv 11, and one contained hpv 16 and 18. papilloma virus common antigen was detected in only three of eight cases, all ... | 1987 | 3037191 |
| human papillomavirus infection of the cervix in victoria, 1982-1985. | cytological evidence of human papillomavirus infection was present in 4.1% of the smear-tests that were received by the victorian cytology (gynaecological) service during 1982-1985. the prevalence rate among women was estimated to be 2.6% in 1984; for one-third of the women this was a change from their previous cytological status. the prevalence of papillomavirus infection was noted to vary with age and referral source; higher proportions were seen in the age group, 20-24 years, and in the cytol ... | 1987 | 3037286 |
| the clinical management and laboratory assessment of anal warts. | the results of a clinical and virological survey of anal warts from 85 predominantly homosexual or bisexual men is presented, together with an improved technique for the surgical treatment of these lesions. two types of anal warts, which were classified as either "acute" or "chronic" on the basis of their appearance and clinical behaviour, were recognized commonly. however, our laboratory investigations--which consisted of the routine histopathological examination of all specimens, together with ... | 1987 | 3037287 |
| differential early viral gene expression in two stages of human papillomavirus type 16 dna-induced malignant transformation. | human papillomavirus (hpv) type 16 dna induces progressive transformation in nih 3t3 cells. two types of cell lines, pm3t3g0 and pm3t3fo, were isolated by g418 or focus selection, respectively, after transfection of cells by a recombinant hpv 16 dna carrying the neo gene. these cell lines exhibited distinct phenotypes compared with controls. saturation densities of pm3t3g0 and pm3t3fo lines were two- to three- and five- to sevenfold greater than that of control nih 3t3 cells, respectively. neith ... | 1987 | 3037348 |
| trichloroacetic acid in the treatment of human papillomavirus infection of the cervix without associated dysplasia. | human papillomavirus-induced cytologic changes were found in 379 (10.9%) of 3461 papanicolaou smears done at the gynecology clinic at wayne state university. of these 379 patients, 98 (25.8%) had no cytologic evidence of dysplasia. forty-six of the 98 patients, who had colposcopic and histologic evidence of human papillomavirus without dysplasia, agreed to participate in this study. all patients underwent a q-tip application of 85% trichloroacetic acid to the entire cervix, including the endocer ... | 1987 | 3037457 |
| human papillomavirus associated with vaginal intraepithelial neoplasia in women exposed to diethylstilbestrol in utero. | five out of 959 young women, exposed to diethylstilbestrol (des) in utero, developed vaginal intraepithelial neoplasia while they were under follow-up in the diethylstilbestrol-adenosis project at baylor college of medicine, houston, texas. we suggest that the development of the vaginal intraepithelial neoplasia at a younger age than usual may be caused by a higher susceptibility of the des-exposed patient to factors associated with the development of intraepithelial neoplasia. a common finding ... | 1987 | 3037458 |
| quantitative deoxyribonucleic acid analysis of patients with mild cervical atypia: a potentially malignant lesion? | quantitative deoxyribonucleic acid (dna) analysis of cervical biopsy material from 32 women with cytologic, colposcopic, and histologic evidence of mild cervical atypia consistent with cervical intraepithelial neoplasia i, reactive atypia, or human papillomavirus infection was carried out using flow cytometry and feulgen microspectrophotometry. evidence of aneuploidy, ie, neoplastic transformation, was demonstrated in all cases of cervical intraepithelial neoplasia i and 68% of cases with human ... | 1987 | 3037459 |