Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| efficacy of cryotherapy in the treatment of human papilloma virus infection of the uterine cervix. | 1986 | 3009684 | |
| immunohistologic analysis of the phenomenon of spontaneous regression of numerous flat warts. | among various tumors induced by human papilloma virus (hpv), flat warts are unique in that they show a systemic regression phenomenon after sudden occurrence of inflammation in all the tumors, leaving permanent immunity to flat warts in the host. when studied immunohistochemically, the presence of hpv antigen using papilloma virus genus-specific antiserum in 31 cases of regressing flat warts was not found; whereas it was demonstrated in the nuclei of upper epidermal cells of ordinary flat warts ... | 1986 | 3017534 |
| human papillomaviruses in buschke-löwenstein tumors: physical state of the dna and identification of a tandem duplication in the noncoding region of a human papillomavirus 6 subtype. | six buschke-löwenstein tumors, i.e., highly differentiated squamous cell tumors of the genital region, were shown to contain human papillomavirus 6 (hpv 6) or hpv 11 genomes. the viral dna was found in an episomal state, including a very small fraction of circular oligomers. hpv 6a and hpv 6d genomes were cloned from two of the tumors. comparison with hpv 6b, cloned from a benign genital wart (e. -m. de villiers, l. gissmann, and h. zur hausen, j. virol. 40:932-935, 1981) by restriction mapping ... | 1986 | 3009899 |
| human papillomavirus and lower genital neoplasia in renal transplant patients. | lower genital cytopathology was evaluated in 105 immunosuppressed renal transplant recipients. evidence of human papillomavirus infection was found in 17.5% and of lower genital neoplasia in 9.5%. the rate of the virus infection in the immunosuppressed was nine times greater than in a general population and 17 times greater than in a matched immunocompetent population. the rate of cervical neoplasia was 16 times greater than in a general population and nine times greater than in a matched immuno ... | 1986 | 3016625 |
| human papillomavirus (hpv) dna sequences demonstrated by in situ dna hybridization in serial paraffin-embedded cervical biopsies. | an in situ dna hybridization technique was used to identify various types of human papillomavirus (hpv) in paraffin sections of serial punch biopsies taken from 64 patients having colposcopy because of abnormal smears. there women were in fact 64 consecutive patients out of 505 attending our clinic (at 6-month intervals) since 1981 for hpv infections. hpv 6 dna sequences were found in 20%, hpv 11 in 17%, hpv 16 in 8% and hpv 18 in 5% of the 64 biopsies analysed with this method so far. more than ... | 1986 | 3017243 |
| time trends in prevalence of cervical cytological abnormality in women attending a sexually transmitted diseases clinic and their relationship to trends in sexual activity and specific infections. | trends in prevalence of cytological evidence of cervical intraepithelial neoplasia (cin) and cervical infection with human papilloma virus (hpv), as indicated by hpv infection and dyskeratosis, were studied in 2,992 new attenders at a sexually transmitted diseases (std) clinic between 1978 and 1982. crude prevalence of cin increased from 1.3% to 4.3% (p less than 0.001) and crude prevalence of hpv infection increased from 2.8% to 9.3% (p less than 0.001). age adjustment had little effect on thes ... | 1986 | 3022781 |
| human papillomavirus infection of the uterine cervix of women without cytological signs of neoplasia. | one hundred and six patients were studied whose cervical smears showed only non-specific inflammatory changes. screening for genital pathogens yielded only a few positive cases. histological examination of biopsy specimens taken by colposcopically directed tissue sampling showed cervical intraepithelial neoplasia in 13 of the women (12.3%). deoxyribonucleic acid (dna) hybridisation techniques were used to detect human papillomavirus, which was found in 24 patients (22.6%). in a second group of 1 ... | 1986 | 3022864 |
| human papillomavirus (hpv) infection of cervical lesions detected by immunohistochemistry and in situ hybridization. | ten cases of cervical dysplasia, 13 cases of carcinoma in situ, nine cases of invasive carcinoma, and six cases of normal tissue were investigated for the presence of papillomavirus group-specific antigens and for viral dna. viral proteins were identified with genus-specific papillomavirus antibodies. cloned hpv 6, 11, and 16 dna labeled with 3h were used for in situ hybridization followed by autoradiographic detection of hybridized probes. in cervical dysplasias, five of ten samples were positi ... | 1986 | 3017555 |
| human papilloma virus in condyloma acuminata of the anus. | 1986 | 3018051 | |
| human papillomavirus deoxyribonucleic acid in adenocarcinoma and adenosquamous carcinoma of the uterine cervix. | this report describes the detection of human papillomavirus type 16 or 18 deoxyribonucleic acid (dna) in nine of 15 invasive tumors of the cervix, including three squamous carcinomas, four adenosquamous carcinomas, one glassy cell carcinoma, and one adenocarcinoma. the viral dna was identified by southern blotting and dna hybridization. human papillomaviruses may play an etiologic role in the development of at least some adenocarcinomas and adenosquamous carcinomas as well as most squamous tumor ... | 1986 | 3016624 |
| [pathological and clinical observations on precancerous lesions of the vulva]. | the vulvar epithelial lesions which have been subjected to histological examinations in nagasaki university hospital (1965-1985) and its 5 affiliated hospitals (1975-1985) included 133 cases in vulvar dystrophies, 72 in other benign lesions, 18 in squamous cell carcinoma in situ, 10 in vulvar paget's disease, and 59 in invasive carcinoma. these lesions were studied pathologically and endocrinologically, and the pathogenesis of vulvar carcinoma and whose clinical problems were discussed. serum ho ... | 1986 | 3018106 |
| the expression of human papillomavirus type 18 e6 protein in bacteria and the production of anti-e6 antibodies. | human papillomavirus type 18 (hpv-18) has recently been closely linked with human malignant cervical lesions. the early region of the genome of the related bovine papillomavirus (bpv) has been shown to be important for the production of the transformed phenotype. bpv e6 has been shown to be a transforming protein. we report the primary structure of the hpv-18 e6 open reading frame and its predicted amino acid sequence. both e6 protein and e6-beta-galactosidase fusion protein were synthesized in ... | 1986 | 3018129 |
| herpes simplex virus and human papilloma virus in the development of cervical carcinoma. | 1986 | 3019599 | |
| dna sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of scotland. | punch biopsy specimens of the cervix were examined both histologically and for the presence of human papillomavirus (hpv) dna sequences. the presence of hpv dna sequences was sought with the southern blot technique using radioactively labelled hpv-6, 11, 16, and 18 dna probes, both together and separately. twenty six biopsy specimens were examined. histological examination showed cervical intraepithelial neoplasia grade 2 or 3 in 16 specimens, viral changes (koilocytosis) in four, and inflammati ... | 1986 | 3015316 |
| human papilloma virus and cervical cancer. | 1986 | 3018462 | |
| immunobiology of human papillomavirus infection. | 1986 | 3018839 | |
| immunohistochemical and electron microscopic demonstration of human papillomavirus in dysplasia of the uterine cervix. | twenty-five cases of dysplasia of the uterine cervix were studied for the presence of human papillomavirus (hpv) by means of immunohistochemical and electron microscopic techniques. serial sections of the same histological specimen were examined in each case. hpv was detected in 14 cases by both immunohistochemistry and electron microscopy, while 10 cases were negative with both methods. in only one case, there was a discrepancy in the results derived from these two methods. it was concluded tha ... | 1986 | 3018963 |
| electron microscopy in assessment of the biological behavior of human papillomavirus infections in the uterine cervix. | to asses the natural history of human papillomavirus (hpv) infections in uterine cervix, currently implicated in etiology of cervical cancer, a prospective follow-up study has been conducted for 418 women at our clinic since 1981. the present communication summarized the current follow-up data of these patients, with special emphasis on detection of the virus in cervical punch biopsies, as correlated with other characteristics pertinent to the clinical behavior of cervical hpv infections. on eac ... | 1986 | 3020455 |
| detection of human papillomavirus type 16 dna and evidence for integration into the cell dna in cervical dysplasia. | the presence of human papillomavirus (hpv) type 16 dna in biopsies from precancerous lesions and from early lesions of human cervical cancer, and the integration of virus dna into host cell dna were analysed by dot blot and southern blot hybridizations. hpv 16 dna was detected in 23% of mild dysplasias, 32% of moderate dysplasias, 55% of severe dysplasias and 62% of carcinomas in situ by dot blot hybridization. digestion of the dna with restriction enzymes psti and bamhi followed by southern blo ... | 1986 | 3018133 |
| the type of human papillomavirus present in cervical infections can be determined by the occurrence of specific marker proteins. | four different proteins have been identified on high resolution two dimensional gels of [35s] methionine labelled human cervical biopsies whose expression correlates with the presence of papillomavirus. they are all basic proteins having molecular weights in the region of 48 to 50 kd and are normally expressed individually in different lesions unless the lesion results from a co-infection of two virus types. comparison of the occurrence of these marker proteins with the actual hpv type present, ... | 1986 | 3024849 |
| differential regulation of papilloma virus early gene expression in transformed fibroblasts and carcinoma cell lines. | treatment of bovine papilloma virus (bpv) 1-transformed mouse fibroblasts with cycloheximide led to a 10-fold increase in the amount of viral transcripts, after as little as 1 h of protein synthesis inhibition. northern blots revealed no qualitative changes in the rna pattern. nuclear run-on experiments showed about a 7-fold increase in specific transcriptional activity after cycloheximide treatment. the half-life of bpv1 mrna was twice as long as in untreated controls. these results indicate th ... | 1986 | 3019673 |
| immunohistochemical demonstration of papillomavirus antigen in cervical dysplasia and vulvar condyloma. | biopsies from 30 cases of vulvar condyloma, 460 cases of cervical dysplasia, 30 cases of carcinoma in situ and 75 cases of invasive carcinoma of the cervix were screened for the presence of human papillomavirus (hpv) antigen by means of the peroxidase-antiperoxidase method. positive reaction for hpv was detected in 14 cases of condyloma and 80 cases of dysplasia as a brown intranuclear precipitate in the superficial layer of the epithelium. none of the cases of carcinoma in situ and invasive can ... | 1986 | 3021598 |
| coexistent chlamydial infections related to natural history of human papillomavirus lesions in uterine cervix. | to assess the role of chlamydia trachomatis in the development of cervical intraepithelial neoplasia (cin) and to evaluate possible synergism between chlamydiae and human papillomavirus (hpv) in this process, 418 women who had been prospectively followed up for cervical hpv infections at our clinic since 1981 were tested for chlamydiae. at each visit the patients were examined by colposcopy, and other investigations, such as papanicolaou (pap) smears, punch biopsies, urethral, and cervical swabs ... | 1986 | 3021608 |
| the frequency of papillomavirus infection in cervical precancerous lesions in japan: an immunoperoxidase study. | in order to establish the frequency of human papillomavirus (hpv) infection in cervical precancerous lesions of japanese patients, cervical materials routinely biopsied in the past year were examined immunohistochemically for the papillomavirus genus-specific antigen. of a total of 832 cervical biopsy specimens, 46 (5.5%) were immunohistochemically positive for hpv. in this study, 206 patients were diagnosed as having dysplasia or carcinoma in situ (cis), and hpv antigen was found in 15% of thes ... | 1986 | 3021698 |
| different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. | transcription of human papillomavirus type 18 (hpv18) dna in the human cervical carcinoma cell lines hela, c4-1 and sw756 was studied by nucleotide sequence analysis of hpv18-positive cdna clones isolated from a hela, c4-1 and sw756 cdna library, respectively, and the cdna sequences were used to predict the potential encoded proteins. the cdna clones from all three cell lines were found to be derived from virus-cell fusion transcripts in which 3'-terminal host cell sequences (different for each ... | 1986 | 3023067 |
| human genital papilloma infections: an evaluation of immunologic competence in the genital neoplasia-papilloma syndrome. | immunologic evaluations of women with genital neoplasia-papilloma syndrome demonstrated the presence of subclinical immunodeficiency when compared with results in 20 control women. all patients with genital neoplasia-papilloma syndrome were previously found to have human papillomavirus deoxyribonucleic acid in genital neoplasias or papillomas occurring either synchronously (in at least two genital organs at the same time) or metachronously (at different times during a period of months to years). ... | 1986 | 3020982 |
| a novel type of human papilloma virus dna from the lesion of epidermodysplasia verruciformis. | two types of dnas were clonally purified from the virion fraction which was obtained by the cesium chloride density gradient centrifugation of a homogenate of scrapings from benign lesions of typical epidermodysplasia verruciformis. restriction-enzyme maps were made for these dnas, and the dna-dna hybridization of these cloned dnas with 25 different types of human papilloma virus dnas indicated that one of the dna clones isolated is a new type of human papilloma virus dna, and the other is the d ... | 1986 | 3023264 |
| detection of human papillomavirus dna by the nucleic acid sandwich hybridization method from cervical scraping. | cervical scrapes collected from 100 consecutive patients participating in a prospective follow-up study for cervical human papillomavirus (hpv) infections were tested for the presence of hpv 11 dna by the nucleic acid sandwich hybridization method, which allows testing the specimens in a crude form. part of each specimen was processed through phenol extraction and dna purification to a dot blot hybridization assay. the dot blots were serially hybridized with hpv 6, 11, 16, and 18 probes as well ... | 1986 | 3023542 |
| human papillomavirus types 16 and 18 sequences in early cervical neoplasia. | a total of 100 colposcopic biopsies from patients with abnormal papanicolau's tests were surveyed for the presence of human papillomavirus (hpv) types 16 and 18 sequences by spot-blot hybridization. hpv 16 and 18 dna sequences were detected in 58% of the biopsies. none of the cervical intraepithelial neoplasia grade i (cin i) contained hpv 16 while 50% of the cin iii lesions (carcinoma in situ, cis) contained hpv 16. hpv 18-related sequences were equally represented in cin i, ii, and iii. southe ... | 1986 | 3022464 |
| detection of human papillomavirus in epithelial cells of uterine cervix-frequency of types 16 and 18 hpv. preliminary results of a clinical cytological and virological study. | 1986 | 3026741 | |
| human papillomavirus and cervical neoplasia: epidemiological considerations. | recent clinical research using new diagnostic and laboratory techniques indicates that human papillomavirus (hpv) infection may play a key role in the development of cervical neoplasia. cervical hpv infection is found in 1-3% of routine papanicolaou smears, and preliminary data suggest that women with cervical hpv infection have about a tenfold greater risk of developing cervical intraepithelial neoplasia (cin) compared with those with no hpv infection. this paper discusses how epidemiological s ... | 1986 | 3007381 |
| the nucleotide sequence and genome organization of human papilloma virus type 11. | the complete nucleotide sequence of human papilloma virus type 11 (hpv11) dna (7931 bp) was determined. hpv11 dna which has been isolated from laryngeal papillomas and from genital warts (condylomata acuminata) shows a high degree of sequence homology to hpv6b (82%). the arrangement of open reading frames is very similar to hpv6b, the homology of the deduced amino acid sequences varies between 58 and 92%. characteristic features of the noncoding region between the l1 and e6 open reading frames i ... | 1986 | 3008427 |
| human papillomavirus dna associated with foreskins of normal newborns. | foreskins from 70 unselected infants undergoing routine circumcision were analyzed by dot blot hybridization for the presence of dna related to that of human papillomavirus (hpv) type 6, 11, 16, or 18. three foreskins (4%) contained hpv dna; two contained dna related to hpv-16, and one contained dna related to hpv-6. these results suggest that neonates exhibit evidence of a relatively high incidence of exposure to hpv. the outcome of exposure may be initially asymptomatic. a retrospective study ... | 1986 | 3009636 |
| [human papillomaviruses in the epithelial cells of the cervix uteri: frequency of types 16 and 18. preliminary results of a clinical, cytologic and viral study]. | between april 1 and june 30 1984, cervical scrapes were taken from 381 women attending the gynecology department of the anticancer center rené-huguenin. the scrapes were examined for the presence of human papillomavirus (hpv) dna, by a molecular hybridization method, at the pasteur institute. the four hpv types involved in genital pathology, hpv 6, hpv 11, hpv 16 and hpv 18, were studied. twenty four specimens (6.3%) were found positive: 19 for hpv 16, 3 for hpv 18, 2 for hpv 6 or hpv 11. result ... | 1986 | 3009600 |
| human papillomavirus dna replication mediated by simian virus 40 t antigen in trans. | the putative e1 gene product of papillomaviruses is thought to be involved in the initiation of viral replication, as large-t antigen (t antigen) is in the case of polyomaviruses. mouse cell lines cloned after transformation by a plasmid consisting of the simian virus 40 (sv40) early region and the complete genome of human papillomavirus type 16 (hpv16) maintained episomal plasmid dna. in contrast, the dnas of either sv40 or hpv16, when employed separately in transfection experiments, were consi ... | 1986 | 3016153 |
| epidermodysplasia verruciformis-associated human papillomavirus 8: genomic sequence and comparative analysis. | human papillomavirus (hpv) 8 induces skin tumors which are at high risk for malignant conversion. the nucleotide sequence of hpv8 has been determined and compared to sequences of papillomaviruses with different oncogenic potential. the general organization of the hpv8 genome is similar to that of other types. highly conserved, genus-specific sequences were found in open reading frames (orfs) e1, e2, and l1. in orfs e6, e7, and l2, hpv8 is more distantly related, but it was possible to differenti ... | 1986 | 3009874 |
| cloning of monomeric human papillomavirus type 16 dna integrated within cell dna from a cervical carcinoma. | we have molecularly cloned and characterized monomeric human papillomavirus type 16 dna with flanking cell dna sequences from a cervical carcinoma. determination of nucleotide sequence around the junctions of human papillomavirus and cell dnas revealed that at the site of integration within cell dna the cloned viral dna had a deletion between nucleotides 1,284 and 4,471 (numbering system from k. seedorf, g. krämmer, m. dürst, s. suhai, and w. g. röwekamp, virology 145:181-185, 1985), which inclu ... | 1986 | 3009901 |
| genome organization and nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer. | the 7,909-nucleotide sequence of human papillomavirus type 33, which is associated with cervical cancer, has been determined and used to deduce the corresponding genome arrangement. extensive sequence homologies and other genetic features are shared with the related oncogenic virus, human papillomavirus type 16, especially in the major reading frames. a surprising difference was found in the noncoding region of human papillomavirus type 33 as, unlike all other sequenced papillomaviruses, it cont ... | 1986 | 3009902 |
| epidermodysplasia verruciformis associated with type ii human papilloma virus. | 1986 | 3009778 | |
| detection of human papillomavirus dna in genital warts, cervical dysplasias and neoplasias. | biopsies from human genital lesions in japan (108 samples), including condyloma acuminata, squamous metaplasia, dysplasia, and cervical cancer, were screened for the presence of human papillomavirus (hpv) 6-, 11-, 16-, and 18-related dnas by spot hybridization and southern blot hybridization under stringent conditions. by spot hybridization, hpv 6/11-related dna was found in 92.9% (13/14) of condyloma acuminata and in 6.7% (1/15) of cervical cancer biopsies. hpv 16/18-related dna was found in 37 ... | 1986 | 3015831 |
| in vivo transformation of human skin with human papillomavirus type 11 from condylomata acuminata. | human papillomaviruses (hpvs) have been implicated in the development of a number of human malignancies, but direct tests of their involvement have not been possible. we describe a system in which human skin from various sites was infected with hpv type 11 (hpv-11) extracted from vulvar condylomata and was grafted beneath the renal capsule of athymic mice. most of the skin grafts so treated underwent morphological transformation, resulting in the development of condylomata identical to those whi ... | 1986 | 3016303 |
| a comparison of the ages of patients with cervical smears showing human papilloma virus, dysplasia and carcinoma in situ changes. | 1986 | 3010201 | |
| human papillomavirus infection of the cervix detected by cervicovaginal lavage and molecular hybridization: correlation with biopsy results and papanicolaou smear. | human papillomaviruses have previously been identified by molecular hybridization in the majority of dysplastic and cancerous lesions of the cervix. since human papillomavirus types 16 and 18 have been strongly associated with cervical cancer, the identification of patients infected with these specific human papillomavirus types may provide useful prognostic information. we have developed a painless, noninvasive cervicovaginal lavage technique to collect exfoliated cervicovaginal cells, which ca ... | 1986 | 3010725 |
| identification of the human papilloma virus-1a e4 gene products. | antibodies prepared against a human papilloma virus-1 (hpv-1) e4/beta-galactosidase fusion protein identified several polypeptides in hpv-1, but not hpv-2 or 4, induced papillomas. the major e4 protein, that represented up to 30% of total cellular protein, was a 16/17-k doublet which was purified by column chromatography and analysed for amino acid content. a peptide derived by chymotryptic digestion was purified by h.p.l.c. and subjected to amino acid sequencing. the unique sequence obtained, g ... | 1986 | 3011404 |
| human papillomavirus dna sequences in penile carcinomas in brazil. | to detect the presence of human papillomavirus (hpv) dna in penile cancers, 18 squamous-cell carcinomas were studied by southern blot analysis. all specimens were from brazilian patients ranging in age from 31 to 78 years. of these, 7 harbored dna sequence homology. most positive specimens contained more than 100 copies of viral dna. no hpv 16 was detected in our samples. presence of hpv dna sequences in the samples studied was not correlated with the patients' age or race, nor with extent or pr ... | 1986 | 3011685 |
| human papilloma virus and cervical cancer. | 1986 | 3012295 | |
| persistence and expression of human papillomavirus dna in genital cancer. | there is mounting evidence that certain types of human papillomaviruses (hpv types 16 and 18) are associated with human genital cancer. other virus types, such as hpv-6 or hpv-11, are more regularly found in benign genital warts. since all viruses can be present in putative precancerous lesions of the uterine cervix (dysplasia, cervical intraepithelial neoplasia) it has been postulated that individual hpv types have different 'oncogenic potential'. the molecular basis for this difference is not ... | 1986 | 3013522 |
| methods for diagnosing papillomavirus infection. | the morphology of the lesion and the site in which the lesion is found are the initial clues in classifying papillomavirus-induced neoplasia. human papillomavirus (hpv) types have limited site-specificity and differ in their association with benign or malignant neoplastic development. cytopathology, electron microscopy, antigen detection and molecular hybridization all play a role in the armamentarium of diagnostic methods. although nitrocellulose blotting procedures provide the most accurate an ... | 1986 | 3013528 |
| [clinical significance of human papilloma virus (hpv) infections of the lower genital tract]. | using filter in situ hybridisation for hpv-dna detection we found among 217 women with positive cervical cytology a positive result in 152 cases (70%). the distribution of the different hpv types showed an association of hpv 6/11 mainly with benign lesions and of hpv 16/18 with obligatory precancer and invasive cervical cancer. in 2652 swabs of cytologically negative patients hpv-dna was identified in 9.5%. the infection rate for hpv 16/18 in pregnant women was 6.4% against 2.3% in nonpregnant w ... | 1986 | 3013712 |
| evaluation of urinary cytology of male sexual partners of women with cervical intraepithelial neoplasia and human papilloma virus infection. | condylomas of the genital tract can be found in 70% of patients who have cytologic evidence of human papilloma virus (hpv) infection or mild epithelial dysplasia (cin i). most male sexual partners of women with overt or subclinical hpv infections have no visible condylomas. presently, there is no therapy for subclinically infected male partners. a screening test capable of detecting such hpv infections in males would be of value should effective therapy be discovered. fifty-four men who were the ... | 1986 | 3013733 |
| identification of human papillomavirus types in oral verruca vulgaris. | eleven oral verruca vulgaris specimens were examined for the presence of papillomavirus structural antigens by reaction with antibody to type-common antigens and detection by the avidin-biotin-peroxidase complex method. the specimens were also examined by in situ hybridization with biotin-labelled human papillomavirus (hpv) dna to determine the specific hpv types present in the lesions. six of the 11 specimens were positive for papillomavirus structural antigens. of these 6, 5 hybridized to the ... | 1986 | 3014099 |
| peroxidase-antiperoxidase evaluation of human oral squamous cell papillomas. | in human beings, the viral origin of condylomatous lesions of the mouth and cervix, multiple papillomas, and verruca vulgaris of the larynx has been established. the viral origin of papillomatosis in rabbits and dogs has also been documented. the literature, however, shows no conclusive evidence of a viral origin of human oral squamous cell papillomas (scp). the purpose of this study was to use the peroxidase-antiperoxidase (pap) staining technique to determine whether human papillomavirus (hpv) ... | 1986 | 3014421 |
| transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the e7 protein. | human papillomavirus type 16 dna and rna were characterized in the cervical cancer-derived caski cell line, which contains only integrated dna, and in a cervical cancer, which contains predominantly plasmid dna. in both, a major rna can code for the early open reading frame e7 and a minor one can code for e6. the cervical cancer, but not the caski cell line, contains a minor rna that can code for an intact e2 protein, and this may relate to the continued presence of plasmid dna. the rna mapping ... | 1986 | 3014503 |
| human papillomavirus in clinically and histologically normal tissue of patients with genital cancer. | to study the association of human papillomavirus (hpv) and herpes simplex virus (hsv) with genital cancer, we collected specimens of cervical, vulvar, endometrial, and vaginal tumors at the time of operation in patients with cancer. in some patients, matched internal-control (histologically normal) tissue was also collected. dna extracted from the tissue was probed with radiolabeled hpv type 16 dna, hpv type 18 dna, and cloned fragments of hsv type 2 dna. hybridization to the hindiiia clone of h ... | 1986 | 3020404 |
| natural killer (nk) cells with hnk-1 phenotype in the cervical biopsies of women followed-up for human papillomavirus (hpv) lesions. | local immunocompetent cell infiltrates in 263 cervical punch biopsies of women followed-up since 1981 (16 +/- 14 months, m +/- sd) for an established human papillomavirus (hpv) lesion with or without concomitant cervical intra-epithelial neoplasia (cin) were analysed for presence of human natural killer (nk) cells, defined by the monoclonal antibody hnk-1 (leu-7) using the avidin-biotin peroxidase complex (abc) technique. hnk-1+ cells remained at a constant low level (1.8 to 3.0% of the cells) i ... | 1986 | 3014803 |
| papillomavirus dna in warts of immunosuppressed renal allograft recipients. | renal allograft recipients were shown to have an increased incidence of warts and skin cancers. we examined 148 patients for evidence of wart virus infections and tested for papillomavirus types, which are known to be associated with human malignancies. of the 148, 36 (24.3%) patients were afflicted with warts at the end of our study period, in contrast to 5 of 148 (3.3%) before transplantation. dna from 16 different biopsies was extracted by phenol treatment for further virological studies. dna ... | 1986 | 3015050 |
| demonstration of human papillomavirus dna in two keratoacanthomas. | 1986 | 3015051 | |
| genital tract papillomavirus infection in children. | genital tract papillomas in five children were examined for the presence of human papillomavirus (hpv) dna by molecular hybridization. papillomavirus dna was detected in each sample and was identified as hpv-6 (three cases), hpv-6 or hpv-11 (one case), or hpv-16 (one case). these viruses are the same as are responsible for genital papillomas (condylomata) of adults. the transmission of adult genital tract viruses to children occurs primarily by a venereal route but may occur by a nonvenereal rou ... | 1986 | 3021069 |
| atypical condylomas of the cervix uteri in singapore women: a histopathological and immunohistochemical study. | subclinical flat cervical warts are commonly recognized by means of colposcopy. these warty lesions frequently contain cellular atypia of a varying degree showing all the features of cervical intraepithelial neoplasia (cin) together with the features of wart virus infection e.g. koilocytosis. human papillomavirus (hpv) antigens and dna have been found in these lesions which can be called atypical condylomas. the presence of such markers in lesions regarded as having neoplastic potential implicat ... | 1986 | 3021107 |
| verrucous carcinoma of the vulva associated with an unusual type 6 human papillomavirus. | an unusually rapid growing vulvar verrucous carcinoma found associated with human papillomavirus type 6 is described. both human papillomavirus structural antigens and deoxyribonucleic acid (dna) sequences were detected in the tumor biopsy. the human papillomavirus genome was isolated and compared with the genome of human papillomavirus type 6b, which was cloned from an anogenital wart. the two genomes were extremely homologous in genomic organization and dna sequence, except for a region on the ... | 1986 | 3003640 |
| bowenoid papulosis of the male and female genitalia: risk of cervical neoplasia. | sixteen patients with bowenoid papulosis (eleven male patients with bowenoid papulosis of the penis and five female patients with bowenoid papulosis of the vulva) were studied clinically, histologically, and virologically and followed up from 12 months to 5 years. in eleven of sixteen cases of bowenoid papulosis, molecular hybridization disclosed the presence of human papillomavirus type 16. in four cases we found new, not fully characterized human papillomavirus, and in two cases, we found both ... | 1986 | 3007587 |
| bilateral and synchronous squamous cell carcinoma of the cervix in a patient with uterus didelphys. | reported herein is the case of a 30-year-old white woman who had a congenitally double uterus with two cervixes (uterus didelphys). each cervix was diffusely involved by squamous cell carcinoma in situ that had progressed to a microinvasive stage of the same depth (0.8 mm) on each side. this coincidence was the more remarkable because it had taken place synchronously. a transmissible coital factor of a viral nature, ie, human papillomavirus infection, was suggested by the presence of koilocytoti ... | 1986 | 3003641 |
| detection of papillomavirus dna in human semen. | human papillomavirus dna has been detected in the semen of three patients, two of whom have severe chronic wart disease. these data support the contention that sexual transmission of human papillomavirus dna could occur via semen, a possibility suggested by epidemiological data on the sexual transmission of human papillomavirus. | 1986 | 3003908 |
| human papilloma virus and cervical cancer. | 1986 | 3003916 | |
| a novel type of human papillomavirus associated with genital neoplasias. | the role of human papillomaviruses (hpvs) in the development of genital neoplasias has been well documented. the genomes of two hpv types, hpv16 and hpv18, have been found to be associated with about 70% of invasive carcinomas of the uterine cervix. as, under non-stringent hybridization conditions, hpv dna sequences have been detected in about 90% of cervical carcinomas, it seems likely that additional hpv types are associated with these tumours. here we report the molecular cloning and characte ... | 1986 | 3012352 |
| genital condylomas in immunosuppressed women: a therapeutic challenge. | of 30 immunosuppressed women with genital condylomas, 20 (67%) had associated neoplasia of the lower genital tract. we retrospectively studied 11 patients with flat condylomas confined to the cervix; all lesions were eradicated by conventional therapy. three patients studied retrospectively and 16 studied prospectively had condylomatous lesions involving several sites within the lower genital tract. these lesions were unusually resistant to various treatment methods. maintenance therapy with 5-f ... | 1986 | 3003926 |
| human papillomavirus deoxyribonucleic acid in cervical carcinoma from primary and metastatic sites. | tissue from 13 cervical cancers and pelvic or para-aortic lymph nodes from the same patient were evaluated by deoxyribonucleic acid hybridization with a human papillomavirus type 16 deoxyribonucleic acid probe for the presence of human papillomavirus-related deoxyribonucleic acid sequences. twelve of the primary malignancies were squamous cancers and one was an adenocarcinoma. eight of the primary tumors contained human papillomavirus type 16 deoxyribonucleic acid sequences, and five contained v ... | 1986 | 3004214 |
| presence of human papillomavirus type 16 related sequences in verrucous carcinoma of the larynx. | verrucous carcinoma of the larynx clinically resembles laryngeal papilloma in that both are wart-like masses on the vocal cords and may be characterized by multifocality and recurrence. human papillomavirus (hpv) infection is an etiological factor in laryngeal papilloma, and recent evidence implicates hpv in squamous neoplasias. to determine whether hpv is also associated with verrucous carcinoma of the larynx, we analyzed tissue specimens from six patients with verrucous carcinoma of the larynx ... | 1986 | 3004723 |
| human papillomavirus type 16 dna-induced malignant transformation of nih 3t3 cells. | a biological function for human papillomavirus 16 (hpv 16) dna was demonstrated by transformation of nih 3t3 cells. hpv 16 dna has been found frequently in genital cancer and has been classified as a papillomavirus on the basis of dna homology. a recombinant hpv 16 dna (pshpv16d), which contains a head-to-tail dimer of the full-length hpv 16 genome, induced morphologic transformation; the transformed cells were tumorigenic in nude mice. expression of transforming activity was unique because of t ... | 1986 | 3003388 |
| langerhans' cells, papillomaviruses and oesophageal carcinoma. a hypothesis. | a hypothesis linking the concept of mucosal immune surveillance (langerhans' cells and intra-epithelial lymphocytes) with recent evidence of human papillomavirus (hpv) infection of the oesophagus in a sequence of events which leads to squamous dysplasia and invasive carcinoma is presented. it is believed that the essential pathway to squamous carcinoma involves an aberration of the langerhans' cell/lymphocyte network and its symbiotic relationship with squamous cells as a result of persistent hp ... | 1986 | 3008355 |
| human papillomavirus infection related to oesophageal carcinoma in black south africans. a preliminary study. | oesophageal specimens derived from 70 patients with established invasive squamous cell carcinoma of the oesophagus were histologically reviewed with special reference to the morphological manifestations of human papillomavirus (hpv) infection. epithelial changes fulfilling the criteria for hpv infection were noted in 23 cases (33%). the presence of hpv antigens was demonstrated by immunohistochemical staining in 7 of these 23 cases. although acceptable for routine diagnostic purposes, histologic ... | 1986 | 3008356 |
| human papillomavirus (hpv) infections of the female genital tract and their associations with intraepithelial neoplasia and squamous cell carcinoma. | human papillomaviruses (hpv) consist of a heterogenic group of viruses (32 different hpv types currently recognized) known to induce a variety of squamous cell tumors (papillomas and warts) in the skin, and on mucous membranes of respiratory, gastrointestinal, and genitourinary tracts. the most familiar hpv manifestation in the genital tract is the venereal wart (condyloma acuminatum) recognized since antiquity, and shown to be a sexually transmitted disease (std). in 1976, two other morphologic ... | 1986 | 3001622 |
| transforming activity of human papillomavirus type 16 dna sequence in a cervical cancer. | a genomic dna sample from cervical cancer tissue, containing human papillomavirus (hpv) type 16, was found to induce malignant transformation of nih 3t3 cells when it was tested by transfection assays using the calcium phosphate coprecipitation technique. the primary and secondary transformants contained the hpv type 16 dna sequences and human specific alu family sequences. to the best of our knowledge, it has not been reported previously that hpv type 16 dna sequences in total genomic dna from ... | 1986 | 3008153 |
| characterization of a new type of human papillomavirus found in a lesion of bowen's disease of the skin. | the genome of a human papillomavirus (hpv) found in a patient with bowen's disease of the skin was molecularly cloned. blot hybridization experiments, performed under stringent conditions, revealed no cross-hybridization between this hpv dna and the other known hpv dnas, showing that this hpv represents a new type, tentatively named hpv34. in relaxed hybridization conditions, the highest cross-hybridization was observed with hpv16 dna. the physical map of hpv34 dna was aligned with the genetic m ... | 1986 | 3003400 |
| human papilloma virus and cervical cancer. | 1986 | 3003546 | |
| oral contraceptives and cervical neoplasia: pooled information from retrospective and prospective epidemiologic studies. | a positive association between the use of oral contraceptives and the risk of cervical neoplasia has been suggested by different sources. this paper examines epidemiologic evidence on this issue through pooled computation of relative risks emerging from 21 studies, subdivided into three main categories: studies based on routine cytologic screening programs, case-control investigations and prospective studies. the pooled estimates of the relative risks for ever vs never use of oral contraceptives ... | 1986 | 3513407 |
| a transmission model for aids. | mathematical and computer-simulation models of sexually transmitted diseases, which have been used to study gonorrhoea and human papilloma virus transmissions, are applied to the study of aids. the problems of adapting the general model to the new problem, within already established principles, are described; and solutions offered. the objective is to predict equilibrium levels for the prevalence and incidence of htlv-iii infection in the u.k. within the uncertainties of the available informatio ... | 1986 | 3792516 |
| failure of etretinate in epidermodysplasia verruciformis. | in a patient suffering from benign lesions of epidermodysplasia verruciformis due to human papillomavirus type 5 little clinical improvement could be observed after 6 months of oral etretinate. the drug was given 75 mg/day for 1 month before dosage was reduced to 50 mg/day. control biopsy after 3 months of treatment still revealed large quantities of virions and typical cytopathic effects in light and electron microscopic examination. | 1986 | 3792602 |
| systemic treatment of condylomata acuminata with recombinant interferon-alpha-2a: low-dose superior to the high-dose regimen. | fourteen patients with chronic genital warts were entered in a pair-matched crossover trial of recombinant interferon-alpha-2a. they received daily doses of either 1.5 x 10(6) u or 18 x 10(6) u interferon for one week by the subcutaneous route (abdominal skin). after an observation period of 4 weeks in case of no change or partial response, patients of the low-dose group were given 18 x 10(6) u and those of the high-dose group 3 x 10(6) u daily for another 7 days. eight of 14 patients had a comp ... | 1986 | 3802956 |
| treatment of recurrent squamous papillomata of the conjunctiva by carbon dioxide laser vaporization. | complete resection of recurrent conjunctival squamous papillomata frequently may be exceedingly difficult, since such lesions are typically friable and multilobulated. recurrent lesions often fill the fornicies and spill onto the lid margins. moreover, since the human papillomavirus (hpv) has been linked to some of these lesions, there remains the further possibility of spread with "cold knife" resection. we discuss the benefits of carbon dioxide laser vaporization therapy for recurrent squamous ... | 1986 | 3093950 |
| quantitative assessment of langerhans' cells in human cervical intraepithelial neoplasia and wart virus infection. | langerhans' cell density was assessed quantitatively in cervical wart virus infection (cervical condyloma), cervical intraepithelial neoplasia, and koilocytic dysplasia with use of an antibody to s100 protein and an indirect immunoperoxidase technique. when compared with normal ectocervix, langerhans' cell density was significantly decreased in cervical wart virus infection and significantly increased in cervical intraepithelial neoplasia. in koilocytic dysplasia, intermediate langerhans' cell d ... | 1986 | 3513580 |
| the treatment of warts. | warts are caused by the human papillomavirus and produce a variety of clinical manifestations, depending on the viral type and the site of the lesion. in this article, the pathophysiology of warts is reviewed briefly, and options for treatment, including liquid nitrogen, dinitrochlorobenzene, bleomycin, podophyllin, duofilm, and surgical excision, are examined. several case studies are also presented. | 1986 | 3538084 |
| in vitro models for studying the molecular biology of carcinogenesis. | although carcinogens cause various similar deleterious effects on rodent and human cells, only rodent cells can convert to malignancy in a quantitative, predictable fashion. therefore, the control mechanisms involving indefinite proliferation and tumorigenicity are different. human cell lines may exhibit normal or aneuploid chromosome constitutions with numerical or structural alterations frequently involving proto-oncogene loci, but fail to produce progressively growing tumors in nude mice. a n ... | 1986 | 3544164 |
| epidemiologic aspects of epidermodysplasia verruciformis (l-l 1922) in japan. | a survey of epidermodysplasia verruciformis (ev), a skin disease caused by human papillomavirus (hpv), was made by means of a questionnaire sent to 92 university hospitals. replies were obtained from 68 hospitals (74% recovery) reporting 66 patients. fewer patients were reported from north-eastern japan than from south-western japan. many ev patients were from families of consanguineous marriages (44%), showing a high incidence of intra-familial onset after consanguineous marriages. the complica ... | 1986 | 3095286 |
| the association between vulvar condylomata acuminata, cervical wart virus infection and cervical intraepithelial neoplasia. | vulvar condylomata acuminata are the commonest clinical expression of human papilloma virus infection of the female genital tract. various epidemiological factors were examined in a group of patients with vulvar warts in whom the incidence of biopsy proven human papilloma virus infection of the cervix was 39.7% and the incidence of biopsy proven cervical intraepithelial neoplasia was increased significantly (16.2%). | 1986 | 3464255 |
| squamous papilloma of the esophagus diagnosed by cytology. report of a case with concurrent occult epidermoid carcinoma. | esophageal material for cytologic smears was obtained by the gel-foam technique from an 84-year-old man with dysphagia and symptoms of obstruction in whom initial attempts at endoscopic biopsy were nondiagnostic. the smears contained cells with koilocytotic atypia, similar to those described in smears from uterine cervix lesions produced by human papilloma virus. the cytologic diagnosis of an esophageal papilloma of viral origin was confirmed by the second biopsy. a third biopsy, from a lower le ... | 1986 | 3465138 |
| evaluation of male consorts of women with genital human papilloma virus infection. | ninety male partners of women with genital condylomata were evaluated for evidence of condylomata by visual examination of the genitalia with the colposcope, urethroscopy, and biopsy. fifty-three men (59%) were found initially to have condylomata and nine other cases were found on reevaluation for a total prevalence of 69%. thorough evaluation with the use of acetic acid, magnification and liberal use of biopsy is stressed. urethroscopy may not be a necessary part of the routine screening evalua ... | 1986 | 3763084 |
| masking effect of hormonal contraceptives on discriminating quantitative features of visually normal intermediate cells in positive and negative cervical smears. | in quantitative studies of visually normal intermediate cells in smears from patients with cervical neoplasia, the contraceptive status of the patients has not previously been taken into account. in this study cervical smears from 151 patients with cervical intraepithelial neoplasia (cin) iii or invasive carcinoma and from 360 normal controls were grouped according to week of menstrual or pill cycle and mode of hormonal contraception. the nuclear-cytoplasmic (n/c) ratio of visually normal interm ... | 1986 | 3778614 |
| risk factors for condyloma acuminatum in women. | we interviewed 49 women who had a history of condyloma acuminatum and 196 age-matched controls. for women who had ever smoked, the risk of developing condyloma was 3.7 times that of women who had never smoked (95% confidence interval, 1.8-7.6). the relative risk associated with long-term use (five or more years) of oral contraceptives was 9.8 (95% confidence interval, 1.5-65.6). condyloma acuminatum is caused by infection with human papilloma virus (hpv), usually types 6 or 11. infection with hp ... | 1986 | 3952595 |
| many oncogenes are transcribed in the d98ah2 derivative of the hela carcinoma cell line. | as a first step in an ongoing study on the correlation of oncogene expression and tumorigenicity in hybrids made by fusing d98ah2 (d98) carcinoma cells with normal diploid human fibroblasts, we determined which oncogenes are expressed in the d98 cells. northern blot determinations of the rna levels of 21 oncogenes revealed moderate to abundant transcripts of 13 oncogenes. the remaining eight oncogenes for which no rna was found normally show a very restricted pattern of expression among cell typ ... | 1986 | 3780321 |
| prophylactic topical 5-fluorouracil following treatment of human papillomavirus-associated lesions of the vulva and vagina. | ninety patients were studied in a controlled, randomized fashion to evaluate the effectiveness of topical 5-fluorouracil in reducing the recurrence rate of human papillomavirus-associated lesions of the vulva and vagina. following complete ablation of the lesions by one or more treatment modalities, 45 patients applied a single dose of 5% 5-fluorouracil cream biweekly to the vagina and/or vulva for at least six months. forty-five patients used no prophylactic 5-fluorouracil cream. patients in bo ... | 1986 | 3785797 |
| correlation of cellular atypia and human papillomavirus deoxyribonucleic acid sequences in exfoliated cells of the uterine cervix. | restriction enzyme digestion and southern blot hybridization were used to analyze deoxyribonucleic acid (dna) extracted from exfoliated cervical cells for the presence of human papillomavirus sequences and these results were correlated with cytologic findings on papanicolaou smears. specimens (n = 204) were obtained from a nonselected population of women undergoing routine cytologic screening and human papillomavirus dna sequences were detected in 33 (16%) women. thirteen smears contained atypic ... | 1986 | 3748500 |
| condylomas of the uterine cervix initially interpreted as squamous carcinoma: a report of four cases including a lesion resembling the buschke-loewenstein giant condyloma. | the recognition of human papilloma virus (hpv)-induced cervicovaginal condylomatous lesions is in a period of dynamic development. it is estimated that condylomatous involvement of the uterine cervix is epidemic and that is particularly true in the younger, more sexually active woman. while cervical condylomas are often associated with cervical dysplasia, carcinoma in situ, or even invasive carcinoma, they are sometimes misdiagnosed as these entities. four cases of cervical condylomas are presen ... | 1986 | 3710268 |
| the pathology of vulvar squamous cell carcinoma and verrucous carcinoma. | squamous cell carcinoma is far more common than verrucous carcinoma in the vulva. the clinical and morphologic distinctions between these neoplasms are important to understand because of their contrasting biologic behavior and treatment. both cancers present with symptoms of pruritus and a noticeable mass. on examination, both tumors commonly occur on the labia and are exophytic. if infection occurs in association with verrucous carcinoma, the resulting induration of the surrounding tissue as we ... | 1986 | 3748617 |
| cytologic detection of condylomas and cervical intraepithelial neoplasia of the uterine cervix with histologic correlation. | human papillomavirus (hpv) has been implicated as an important etiologic factor in cervical carcinoma. this study evaluates the efficacy of cytology as a screening tool in the detection of cervical lesions with koilocytotic features. cervical smears and biopsy specimens from 76 women seen between january 1983 and october 1985 were reviewed. the histologic categories consisted of koilocytotic lesions (flat condylomas) with minimal cellular atypia, cin i, ii, iii, with surface koilocytes showing c ... | 1986 | 3756823 |
| [etiology of cancer of the cervix uteri. an account of 150 years' research]. | one hundred and fifty years ago, a descriptive study showed that marriage was a risk factor in cervical carcinoma. more recent analytical epidemiological studies have shown that this carcinoma was a sexually transmitted disease. great strides in molecular biology have made it possible to assign to some human papillomavirus form a strong aetiological role in the disease. | 1986 | 3517123 |
| carbon dioxide laser vaporization of cervical subclinical papillomaviral infection and intraepithelial neoplasia: short-term effectiveness. | 134 patients with biopsy-proved cervical subclinical papillomaviral infection (spi) (the so-called 'flat condylomas'), or with associated intraepithelial neoplasia (also called 'atypical condyloma' or 'cin with features of condyloma'), or with isolated, apparently 'non-warty' intraepithelial neoplasia were treated by means of the carbon dioxide laser between july, 1982, and january, 1985, following a planned approach and on an outpatient clinic basis, without the use of anesthesia or analgesia. ... | 1986 | 3028881 |
| human papillomavirus 25-related dna in solitary keratoacanthoma. | solitary keratoacanthomas of 32 patients were screened for the presence of human papillomavirus (hpv) 25 dna, which was originally isolated and molecularly cloned from warts of an epidermodysplasia verruciformis (ev) patient. biotinylated virus dna was hybridized in situ to thin sections obtained from paraffin-embedded material. hpv dna was detected in 12 of 32 tumors under stringent conditions, and in 2 additional tumors under relaxed conditions. | 1986 | 3032117 |
| human papillomavirus dna detected in squamous-cell carcinoma tissue of a patient with epidermodysplasia verruciformis. | 1986 | 3028285 | |
| human papillomavirus type 2 dna in oral verrucous carcinoma. | tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (hpv). the tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of hpv dna was determined by in situ hybridization with biotin-labelled hpv dna probes. seventeen tissue specimens were obtained from 9 patients, and included pre-malignant lesions and primary and recurrent tumors. one pre-malignant lesion was positive ... | 1986 | 3027288 |