Publications

different papillomaviruses as the causes of oral warts.	we have observed four patients with oral papillomas. two children had oral mucosal lesions characteristic of focal epithelial hyperplasia, a young man had common, wart-like lesions on his hard palate, and a male immunosuppressed renal allograft recipient had condyloma-like lesions on his gingivae. papillomavirus-like particles were seen by electron microscopy in lesions from both patients with focal epithelial hyperplasia. no structural antigens for human papillomavirus (hpv) 1, 2, 3, or 5 were ...	1982	6284061
human papilloma viral dna replicates as a stable episome in cultured epidermal keratinocytes.	human papilloma virus (hpv) is poorly understood because systems for its growth in tissue culture have not been developed. we report here that cultured human epidermal keratinocytes could be infected with hpv from plantar warts and that the viral dna persisted and replicated as a stable episome. there were 50-200 copies of viral dna per cell and there was no evidence to indicate integration of viral dna into the cellular genome. there was also no evidence to suggest that viral dna underwent prod ...	1982	6285348
[detection and mapping of conserved nucleotidic sequences between the genomes of human papillomavirus 1 a and bovine papillomavirus 1 by electron microscope heteroduplex analysis (author's transl)].	three regions of partial homology have been detected between the genomes of human papillomavirus 1 a (hpv-1 a) and bovine papillomavirus 1 (bpv-1) by electron microscope analysis of heteroduplex molecules. these regions contain about 25% of non-homologous bases and represent 13% of the genome length. they have been mapped on the viral dnas. this allowed a reciprocal orientation of the physical maps of the two genomes. two regions are located in the middle of the transforming fragment of bpv-1 dn ...	1982	6286053
a comparative study on ultrastructure and immunological properties of human papilloma virus before and after fixation.		1982	6286744
analysis of human genital warts (condylomata acuminata) and other genital tumors for human papillomavirus type 6 dna.	32p-labelled cloned hpv 6 dna was used as probe to analyze human genital tumors for dna sequences homologous to hpv 6 dna. ninety three percent of all condylomata acuminata (41 out of 44) were found to harbor hpv 6 dna. of the remaining three, one contained hpv 1 dna. no papillomavirus dna was identified in the two other tumors. all three invasively growing giant condylomata acuminata (buschke-löwenstein tumors) investigated also contained hpv 6 dna. two out of six atypical condylomata of the ce ...	1982	6277807
frequency and distribution of papillomavirus structural antigens in verrucae, multiple papillomas, and condylomata of the oral cavity.	sixty-seven proliferous squamous epithelial lesions of the oral cavity were examined for the presence of human papillomavirus whole (structural) virion antigens by a peroxidase-antiperoxidase technique having immunospecificity against genus-specific (common) antigens of the papillomaviruses. a positive reaction for papillomavirus genus specific antigens was found in 18 of 29 verrucae, 2 of 5 multiple papillomas, and 3 of 5 condylomata; common antigens were not detected in 28 keratoacanthomas. th ...	1982	6282128
intraepithelial squamous lesions of the vulva: biologic and histologic criteria for the distinction of condylomas from vulvar intraepithelial neoplasia.	we reviewed 65 intraepithelial lesions of the vulva and distal vagina and compared the presence of koilocytosis, abnormal mitoses, and parabasal or basal nuclear enlargement with dna microspectrophotometric distribution patterns and the presence of human papillomavirus antigen as determined by immunoperoxidase. abnormal mitoses and cytologically atypical nuclear enlargement were specific predictors of aneuploidy and were reliable for distinguishing vulvar intraepithelial neoplasia (vin) from con ...	1982	6287842
early invasive and in situ warty carcinoma of the vulva: clinical, histologic, and electron microscopic study with particular reference to viral association.	cases of carcinoma of the vulva with a warty appearance were reviewed. altogether, 27 cases of warty carcinoma of the vulva were treated at the university of minnesota hospitals between 1976 and 1980, which accounted for 22.4% of all vulvar epithelial malignancies. as compared to the previous experience between 1951 and 1970, this relative frequency increased fourfold. clinical history indicated that warty carcinoma of the vulva was not necessarily preceded by long lead periods of in situ lesion ...	1982	6285742
demonstration of human papilloma virus antigen in the condylomatous lesions of the uterine cervix by immunoperoxidase technique.	routine histological sections of characteristic condylomas (showing dysplasia of different degrees) collected from the uterine cervix of 10 women were subjected to staining with indirect immunoperoxidase technique using antihuman papillomavirus (anti-hpv) serum to demonstrate the hpv antigens in these lesions. the anti-hpv immune serum was raised in guinea pigs immunized with highly purified viruses from a pool of skin wart lesions collected from various anatomical sites. 8 out of the 10 condylo ...	1982	6288519
isolation of a human papillomavirus from a patient with epidermodysplasia verruciformis: presence of related viral dna genomes in human urogenital tumors.	the dna genome of a human papillomavirus (hpv), tentatively designated hpv-ev, was molecularly cloned from hand to leg lesions of a patient with epidermodysplasia verruciformis, a chronic skin disease associated with a 30% risk of developing cancer. using stringent hybridization conditions, we observed less than 5% homology between hpv-ev and the cloned genomes of hpv-1, hpv-4, hpv-5, and hpv-5a. hpv-ev dna showed approximately 6% homology with hpv-2 and 36% homology with hpv-3. these data sugge ...	1982	6289302
human papillomavirus. frequency and distribution in plantar and common warts.	fifty-nine (24 plantar, 35 common) warts surgically excised from 44 patients (15 female, 29 male), 18 to 32 years old, were examined by electron microscopy (em) for papillomavirus particles and by fluorescent antibody (fa) and peroxidase-antiperoxidase (pap) tests for human papillomavirus type 1 (hpv-1)-specific antigens and papillomavirus genus-specific (common) antigens. fifty per cent of plantar warts and 52 per cent of common warts were positive for hpv by em. when examined by fa and pap for ...	1982	6290785
viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.	juvenile- and adult-onset laryngeal papillomas were examined for the presence of a human papillomavirus (hpv) genome and capsid antigens. dna was isolated from a portion of tissue removed for therapeutic purposes, and the presence of a papillomavirus genome was detected by southern transfer analysis. the viral dna found in the 12 juvenile-onset and the 8 adult-onset laryngeal papillomas examined was identified as hpv-6 on the basis of size, restriction endonuclease digestion patterns, and homolo ...	1982	6291043
molecular cloning and characterization of human papilloma virus dna derived from a laryngeal papilloma.	papilloma virus dna from a laryngeal papilloma was cloned in phage lambda l 47 and characterized after cleavage with different restriction enzymes. hybridization with the dnas of human papilloma virus types 1, 2, 3, 4, 5, and 8 showed no homology under stringent hybridization conditions. human papilloma virus type 6 dna, however, was partially identical to laryngeal papilloma virus dna; different restriction enzyme fragments hybridizing with the other dna were identified on each genome. the degr ...	1982	6292500
human papilloma virus (hpv) antigens in lesions of laryngeal squamous cell carcinomas.	paraffin sections from 36 laryngeal squamous cell carcinomas with histological features of coexistent condylomatous changes (the flat, inverted and papillomatous lesions caused by human papilloma virus; hpv) were stained for hpv antigens with an indirect immunoperoxidase technique. the hpv antiserum used was prepared in guinea pigs immunized against the purified hpv virions from human common wart tissue. of the tumors studied, 36% showed the presence of hpv antigens. in that, one-third of the fl ...	1982	6292810
detection of human papillomavirus dna in anogenital neoplasias.	the presence of papillomaviruses in epithelial-derived cancers from several animal species has led to the speculation that these viruses may also have a pathogenic role in the development of certain human carcinomas, particularly those associated with the anogenital tract. recently, human papillomavirus (hpv) dna has been detected in epithelial-derived cancers, both cutaneous and metastatic, from patients exhibiting the rare, chronic flat wart disease, epidermodysplasia verruciformis (ev). excep ...	1982	6294530
squamous cell papilloma of the esophagus: a tumour probably caused by human papilloma virus (hpv).	the third well-documented case of an oesophageal squamous cell papilloma in the literature is studied by light microscopy and using an indirect immunoperoxidase-pap technique. human papilloma virus (hpv) antigens were found within the nuclei of the superficial dyskeratotic cells and of koilocytes, both of which are characteristic cells found in hpv lesions elsewhere in the body. the findings are discussed in the light of current concepts of hpv lesions in general. it is suggested that this tumou ...	1982	6188592
correlation between human papillomavirus (hpv) type and histology of warts.	forty warts from different patients and of different clinical type were examined histologically and virologically. eight lesions were found to be associated with human papillomavirus type 1 (hpv 1), 15 tumors were induced by hpv 2, hpv 3 was detected 4 times, hpv 4 twice, and hpv 6 eleven times. hpv 3, hpv 4, and hpv 6 induced warts revealed a correlation between histology and virus type. they are characterized by the so called "edematous type clear cells". in hpv 3 associated flat warts pycnoti ...	1982	6276473
carcinoma cuniculatum (verrucous carcinoma of the skin): a clinicopathologic study of 46 cases with ultrastructural observations.	forty-six examples of carcinoma cuniculatum, a rare variant of squamous cell carcinoma of the skin, were studied by means of light and electron microscopy and immunoperoxidase techniques. the tumors usually presented as ulcerated, fungating, and polypoid masses with openings of sinus tracts onto the skin surface that exuded foul-smelling greasy material when pressure was applied. on microscopic examination the tumors were seen to be composed mainly of mature squamous keratinocytes with foci of c ...	1982	7042070
human papillomavirus infection of the uterine cervix.		1982	6307901
histologic evidence for an association of cervical intraepithelial neoplasia with human papilloma virus infection.	all the cytologic and histologic material pertaining to 100 patients who underwent cervical conization for advanced cervical intraepithelial neoplasia (cin) was reviewed. the revision of the histology of the biopsies and cones showed in 56 cases the association of cin with viral cytopathic effects (vce) attributable to human pappiloma virus (hpv) and in 52 the coexistence of a predominantly flat condyloma. the comparison of the two groups of cin, with and without vce, showed that in the first th ...	1982	6299673
[clinical and histological characteristics of the changes induced by human papilloma virus (hpv 7)].		1982	6312492
human papillomavirus 1a complete dna sequence: a novel type of genome organization among papovaviridae.	the complete nucleotide sequence of human papillomavirus type 1a (7811 nucleotides) has been established. the overall organization of the viral genome is different from that of other related papovaviruses (sv40, bkv, polyoma). firstly, genetic information seems to be coded by one strand. secondly, no significant homology is found with sv40 or polyoma coding sequence for either dna or deducted protein sequences. the relatedness of human and bovine papillomaviruses is revealed by a conserved codin ...	1982	6325156
immunoperoxidase demonstration of human papilloma virus (hpv) in dysplastic lesions of the uterine cervix.	routinely processed sections of 47 unselected cervical dysplasias and in situ carcinomas (both condylomatous, co, and noncondylomatous, nco) were subjected to staining with indirect immunoperoxidase-pap technique using anti-human papilloma virus (anti-hpv) immune serum to demonstrate hpv antigens in these lesions. anti-hpv was raised in guinea pigs immunized with highly purified virions from a pool of human skin wart tissues. positive reactivity for hpv (brown intranuclear precipitates) was disc ...	1982	6299212
[clinical and histological characteristics of the changes induced by hpv 4 (human papilloma virus)].		1982	6312491
[the immune states of patients with herpes simplex virus and human papilloma virus infections. case reports].		1982	6315570
cervical intraepithelial neoplasia and 'wary' atypia: a study of colposcopic, histological and cytological characteristics.	colposcopic, histological and cytological characteristics of 168 consecutive patients attending a colposcopy clinic were reviewed. a group of patients demonstrated a cluster of histological features previously related to possible human papilloma virus infection of cervical epithelium, including particularly koilocytosis, double nucleation and dyskeratosis. these patients had a significantly greater occurrence of colposcopic 'finger-like' lesion outline and colposcopic impression of warty change, ...	1982	7093173
verruca vulgaris of the larynx: a distinctive lesion of probable viral origin confused with verrucous carcinoma.	two adult males had superficial, keratotic vocal cord lesions. one was diagnosed as verrucous carcinoma, prompting a hemilaryngectomy. the other was interpreted as a squamous papilloma and was treated by local excision. both patients are disease-free 7 years and 18 months, respectively, following surgery. the histologic features of both lesions are identical to those of cutaneous verruca vulgaris, and immunoperoxidase staining for human papilloma virus was positive in one case. laryngeal verruca ...	1982	7114361
histological changes identical to those of condylomatous lesions found in esophageal squamous cell carcinomas.	esophageal specimens derived from sixty patients with an established invasive squamous cell carcinoma of the esophagus were histologically surveyed with special reference to the morphological manifestations of human papilloma virus (hpv), known to cause flat, inverted and papillomatous lesions (condylomas) in the uterine cervix. epithelial changes fulfilling the criteria of the papillomatous condyloma were present in one case, those of the inverted lesions in three cases, and those of the flat c ...	1982	7138240
human papillomavirus 1a complete dna sequence: a novel type of genome organization among papovaviridae.	[this corrects the article on p. 231 in vol. 1, pmid: 6325156.].	1982	16453421
human papillomavirus infection of the lower female genital tract: association with multicentric neoplasia.	fifty-four patients with multiple condylomatous lesions and/or multicentric neoplasia were identified in a consecutive series of 2,627 colposcopic examinations (2%). forty-one percent presented with condylomata only and 57% exhibited associated intraepithelial or invasive neoplasia in at least one site. only one patient was found to have multicentric neoplasia with no condylomatous features. there was a significant increase in mean age between patients presenting with multiple condylomatous lesi ...	1983	6315610
human papillomavirus type-16-related dna in genital bowen's disease and in bowenoid papulosis.	32p-labelled dna of hpv 16 which has been isolated and molecularly cloned from a cervical carcinoma (dürst et al., 1983) was used to screen the cellular dnas obtained from 20 different biopsies of morbus bowen or bowenoid papulosis, respectively, by southern blot analysis. under conditions of differing stringency for the hybridization, hpv 16 dna or related sequences were identified in 6 out of 10 cases of morbus bowen (4 out of 5 from a genital localization) and in 8 out of 10 biopsies from bow ...	1983	6315601
human papillomavirus and cervical lesions.	human papillomavirus (hpv) infection has been implicated in the intraepithelial cervical changes that cause most abnormal papanicolaou smears. to date, 14 types of hpvs have been identified. all are small, nondeveloped, icosahedral dna viruses that share a common internal antigen. in cases of cervical hpv infection, the koilocytes and dyskeratocytes are the most frequently seen cell types. most infections are flat aceto-white lesions. florid condyloma acuminatum, usually detectable with the ...	1983	6319060
colposcopy in the diagnosis of papillomavirus infection of the uterine cervix.	morphological criteria are described for the diagnosis of papillomavirus lesions at colposcopy. using these criteria, evidence of cervical papillomavirus infection was found in 58 (29.0%) of 200 women referred for colposcopy because of an abnormal cytology report. directed biopsies were obtained from 152 of these patients and histological changes suggestive of papillomavirus infection were found in 47 (30.9%); additional biopsy material from 139 was studied using an immunohistochemical technique ...	1983	6315049
oncological consequences of impaired immune surveillance against ubiquitous viruses.	the immune system is constantly challenged by ubiquitous viruses. multiple immune defenses have evolved to meet these challenges, and thus immunocompetent individuals successfully respond to infection without sequela. x-linked lymphoproliferative syndrome patients, renal allograft recipients, and acquired immunodeficiency syndrome patients share impaired immune surveillance as a common feature. such individuals are variously susceptible to numerous untoward complications following infection with ...	1983	6309892
abnormalities of the uterine cervix in women with vulval warts. a preliminary communication.	fifty women attending a clinic for sexually transmitted diseases with vulval condylomata acuminata were examined by cervical cytology and colposcopy for cervical infection by human papillomavirus (hpv) or epithelial abnormality indicating cervical intraepithelial neoplasia (cin) or both. collated results showed a high prevalence of both conditions in these 50 women; 25 (50%) had evidence of cervical infection by hpv and 18 (36%) epithelial abnormalities consistent with cin 1 or 2.	1983	6299450
genital condyloma virus infection following pelvic radiation therapy: report of seven cases.	six women who underwent radiation therapy for gynecologic malignancies demonstrated cytologic evidence of condyloma virus infection 2 or more years following radiation. histologic confirmation was obtained in two of the cases. a seventh patient developed in situ and invasive squamous cell carcinoma in a vulvar condyloma acuminatum following radiation therapy for hodgkin's disease. this venereal infection is found most frequently in sexually active younger women (average age, 27 years). it is fel ...	1983	6315611
demonstration of human papilloma virus (hpv) antigens in a case of urethral condyloma.	a 33-year-old women is reported, in whom a urethral condyloma was found 2 years after a preceding condylomatous lesion in the uterine cervix. the urethral condyloma was of the flat type, not previously described in this location, and it was found by an indirect immunoperoxidase-pap method to contain cells positive for human papilloma virus (hpv) antigens. the role of hpv in the etiology of such condylomas is briefly reviewed and the suggested involvement of hpv in human squamous cell carcinogene ...	1983	6316481
the story of a cell. the george n. papanicolaou award lecture.	the history of one cell type observed on gynecologic cytologic samples and later identified as pathognomonic of human papillomavirus (hpv) infection is presented. mention is also made of the role of hpv in the development of premalignant and malignant squamous lesions of the uterine cervix.	1983	6316699
immunocompetent cells in uterine cervical lesions of human papillomavirus origin.	to elucidate the local immunological reactions operative in the human papillomavirus (hpv)-induced lesions of the uterine cervix, a total of 150 cervical biopsies containing a dysplastic or a neoplastic change with (co, 97 cases) or without (nco, 53 cases) a coexistent hpv lesion (a flat, an inverted or a papillomatous condyloma) were subjected to anae (acid alpha-naphthyl acetate esterase) staining of the immunocompetent cells; b and t lymphocytes, mononuclear phagocytes (mps cells). in additio ...	1983	6317528
human papillomavirus: detection of viral dna sequences and evidence for molecular heterogeneity in metaplasias and dysplasias of the uterine cervix.	colposcopic-directed biopsies of the uterine cervix from 22 patients were analyzed for the presence of human papillomavirus (hpv) dna and structural antigens. 11 of the biopsies were classified microscopically as mild dysplasia, 3 as moderate dysplasia, 1 as severe dysplasia, and 7 as squamous metaplasia. nonstringent hybridization with a bovine papillomavirus type 1 dna probe and immunocytochemical analysis with an antiserum against papillomavirus genus-specific structural antigens were perform ...	1983	6317605
morphological and immunohistochemical evidence suggesting human papillomavirus (hpv) involvement in oral squamous cell carcinogenesis.	human papillomavirus (hpv) is an agent responsible for squamous cell tumors (verrucae, condylomata and papillomas) at various sites of the body, the oral cavity included. due to the recently pointed-out association between these hpv lesions and the squamous cell dysplasias and malignancies in the uterine cervix, in the bronchus and in the larynx, the present work was carried out to assess, whether morphological signs (cytopathic effects of hpv) or hpv antigens could be found in lesions of oral s ...	1983	6325356
the prevalence of human papillomavirus antigen in patients with cervical intraepithelial neoplasia.		1983	6307333
t-cell subsets and langerhans cells in wart lesions.	the presence of t-lymphocytes and langerhans cells was assessed on tissue sections of 28 human warts from various localizations and in normal epidermis by indirect immunofluorescence using specific monoclonal antibodies. in most cases, the density of langerhans cells was dramatically reduced in wart epidermis as compared to normal epidermis and a mild cellular infiltrate could be noted. in some lesions, okt4 and okt8 cell populations were present and the number of langerhans cells was high both ...	1983	6309654
natural history of cervical epithelial abnormalities in patients with vulval warts. a colposcopic study.	the natural history of intraepithelial abnormalities of the cervix associated with human papillomavirus infection was investigated in a prospective study of 50 women with vulval warts, of whom 28 had colposcopic evidence of a cervical epithelial abnormality and 22 a normal cervix. of the 28 with a cervical abnormality, 26 were re-examined by colposcopy after three months; the epithelial abnormality had persisted in 23 women. nineteen women who had initially shown abnormality by colposcopy were r ...	1983	6311324
genital warts and cervical cancer. ii. is human papillomavirus infection the trigger to cervical carcinogenesis?	evidence of a biologically significant association between subclinical papillomavirus infection (spi) and cervical neoplasia raises the question of whether this is a causal relationship. human papillomavirus (hpv) infection of metaplastic epithelium in the cervical transformation zone is relatively common, producing latent infection in susceptible persons. the epidemiological characteristics of spi and cervical cancer are essentially identical and there is a strong clinico-pathological associati ...	1983	6299907
[detection of human papillomavirus type 6 dna genome in condyloma acuminata].		1983	6300261
molecular cloning of a new human papilloma virus isolated from epidermodysplasia verruciformis lesions.		1983	6300314
human papillomavirus types 6 and 11 dna sequences in genital and laryngeal papillomas and in some cervical cancers.	human genital tumors as well as recurrent laryngeal papillomas were analyzed for the presence of human papillomavirus (hpv) 6 and hpv 11 sequences. hpv 11 dna was found in 7 of 14 laryngeal papillomas; in the 7 other tumors no hpv dna was demonstrated. hpv 11 dna was also found in all five atypical condylomata of the cervix included in this study. condylomata acuminata mainly contained hpv 6 dna. from 63 biopsy specimens, 41 clearly harbored hpv 6 dna and 13 harbored hpv 11 dna. in three tumors ...	1983	6300854
human papilloma virus in multifocal, invasive female genital tract malignancy.	two young patients with multifocal, invasive, verrucoid carcinomas of the lower genital tract are described. viral particles consistent with human papilloma virus were demonstrated by transmission electron microscopy in areas of koilocytotic malignant and nonmalignant epithelium, and both patients had evidence of impaired immune function. the role of human papilloma virus in genital neoplasia is discussed.	1983	6308532
human papillomavirus in cervical condylomata. an immunohistochemical study.	twenty-seven cervical condylomata were studied by morphological and immunohistochemical methods (peroxidase-antiperoxidase technique according to sternberger, with some modifications). the antiserum was obtained from rabbits immunized by human papillomavirus virions; 37% of condylomata stained positively and the koilocytotic cells showed a dark brown nuclear stain. this technique, not particularly useful for diagnostic purposes, could be employed to obtain better understanding of the natural his ...	1983	6312546
association of human papillomavirus infection with cervical intraepithelial neoplasia.	human papillomavirus infections of the cervix were assessed in patients attending a colposcopy clinic. of 348 patients with cervical biopsies, 134 (38.5%) had human papillomavirus infections, and of 251 patients with cervical intraepithelial neoplasia (cin) 112 (44.6%) had human papillomavirus. the majority of patients with human papillomavirus had concurrent cin (83.6%; 112/134). patients with human papillomavirus were significantly younger than patients without human papillomavirus (24.4 versu ...	1983	6310463
presence of human papillomavirus dna sequences in cervical intraepithelial neoplasia.	twenty two patients referred to a district colposcopy clinic because of an abnormal cervical cytology report or a suspicious cervix and found to have a cervical epithelial abnormality were studied. the techniques of cytology, histology, immunohistochemistry, and dna-dna hybridisation were used to detect infection by human papillomavirus. using an indirect immunoalkaline phosphatase technique human papillomavirus antigen was found in biopsy specimens from six of the 22 patients and dna of papillo ...	1983	6311316
characterization of human papillomavirus type 13 from focal epithelial hyperplasia heck lesions.	focal epithelial hyperplasia heck lesions of a turkish patient were shown to contain papillomavirus-specific dna, which was molecularly cloned into bacteriophage lambda. it proved to be related to human papillomavirus (hpv) type 6 dna and hpv type 11 dna. reassociation kinetics revealed a cross-hybridization of 4 and 3%, respectively. there was no cross-reactivity with hpv type 1, 2, 3, 4, 5, 8, or 10. this papillomavirus type will be referred to as hpv type 13. the dna was characterized by clea ...	1983	6312071
identification of human papillomavirus dna in cervical and vaginal intraepithelial neoplasia with molecularly cloned virus-specific dna probes.	the presence of human papillomavirus (hpv) dna was identified in the tissues of cervical and vaginal intraepithelial neoplasia by southern blot dna hybridization under conditions of low stringency. the specific types of hpv present in the tissues were identified by using molecularly cloned types 1 through 6 (hpv-1 through hpv-6) hpv dna probes under high-stringency conditions. all tissues of cervical and vaginal intraepithelial neoplasia analyzed contained hpv genomes. fifteen of 19 samples (79% ...	1983	6313534
human papillomaviruses associated with epidermodysplasia verruciformis. ii. molecular cloning and biochemical characterization of human papillomavirus 3a, 8, 10, and 12 genomes.	the dnas of four human papillomaviruses (hpvs) that were found in the benign lesions of three patients suffering from epidermodysplasia verruciformis have been characterized. the flat wart-like lesions and the macular lesions of patient 1 contained two viruses, hpv-3a and hpv-8, respectively, whose genomes had previously been only partially characterized. the flat wart-like lesions of patient 2 and the macular lesions of patient 3 each contained a virus previously considered as belonging to type ...	1983	6312091
distribution of human papillomavirus antigen in cervicovaginal smears and cervical tissues.	an immunoperoxidase study, using the avidin-biotin complex method and a cross-reactive, antihuman papillomavirus (hpv) antiserum, was carried out on paired papanicolaou-stained cervicovaginal smears and tissue sections from 45 cases of histologically diagnosed genital condyloma. there was a close correlation between the results of smears and cervical tissue sections. the smears were positive for intranuclear hpv antigen(s) in 67% of the cases and the tissues in 62% of the cases. both cervical sm ...	1983	6313535
human papillomavirus lesions in association with cervical dysplasias and neoplasias.	a series of 620 cervical biopsy specimens (precancerous and malignant) was assessed morphologically with special reference to the concomitant appearance of human papillomavirus lesions. tissue samples from 346 of these biopsy specimens were stained for human papillomavirus antigens using the immunoperoxidase-peroxidase-anti-peroxidase (pap) technique. papillomavirus lesions were found in 55.6% of the biopsy specimens associated with all degrees of epithelial atypia. the mean age of the women wit ...	1983	6312387
new approaches for computer analysis of nucleic acid sequences.	a new high-speed computer algorithm is outlined that ascertains within and between nucleic acid and protein sequences all direct repeats, dyad symmetries, and other structural relationships. large repeats, repeats of high frequency, dyad symmetries of specified stem length and loop distance, and their distributions are determined. significance of homologies is assessed by a hierarchy of permutation procedures. applications are made to papovaviruses, the human papillomavirus hpv, lambda phage, th ...	1983	6577449
[focal epithelial hyperplasia in lepromatous leprosy].	focal epithelial hyperplasia heck (feh) is most likely caused by human papilloma virus. it mainly occurs in children and young people showing no associated diseases. for the first time, we describe a case of feh in a patient with lepromatous leprosy who due to persistent erythema nodosum leprosum has been treated with a lang-term glucocorticoid therapy. the question of the competence of lepromatous patients in resisting certain viral infections arises.	1983	6649746
human papilloma virus infection (condyloma) of the cervix and cervical intraepithelial neoplasia: a histopathologic and statistical analysis.	two hundred fifty consecutive cases previously diagnosed as advanced intraepithelial neoplasia (cin 2 and cin iii) were reviewed and examined for the presence of coexisting condyloma. when the mean ages and age distributions were analyzed, there was a consistent shift of the age distribution to a younger age among women with cin and condyloma as opposed to cin alone. furthermore, when the relationship between age and case accumulation was normalized from a curvilinear to a linear relationship, t ...	1983	6822369
effect of oral aromatic retinoid (ro 10-9359) on human papilloma virus-2-induced common warts.	persisting human papilloma virus (hpv) 2 induced common warts of a chronic lymphatic leukemia patient were orally treated with aromatic retinoid ro 10-9359 (tigason). clinically, the lesions improved rapidly. virus-specific cytopathogenic effects (cpe), virus particles and viral dna were no longer detectable. therapy was discontinued because of the development of a liposarcoma, which led to a complete relapse of the cutaneous lesions. hpv-2 specific parameters, cpe and viral dna, were restored. ...	1983	6840376
[false forms of bowen disease of the genital mucosa].	this review deals with the lesions of the genitalia histologically mimicking bowen's disease, yet behaving like a benign process. the report is based on data from the literature and on 14 personal cases, four of which are described here as examples. some of the cases, mostly young patients, showed spontaneous regression; in others condylomata acuminata developed on the site of the lesions; in rare instances a "transformation" in lichen planus occurred. for these not really precancerous condition ...	1983	6874339
local immunological reactivity in oral squamous cell lesions of possible hpv (human papillomavirus) origin.	the immunocompetent cells (b and t lymphocytes, mononuclear phagocytes, mps cells) known to be responsible for the rejection of the hpv- (human papillomavirus) induced tumors in the skin (warts) were analyzed in the infiltrates of 63 oral squamous cell lesions of suspected hpv origin (condyloma, ca; focal epithelial hyperplasia, feh; squamous cell papilloma, sqp) as well as in tumors thought to be unrelated to hpv (fibrous hyperplasia, fh; papillary hyperplasia, ph; true fibroma, tf; keratoacant ...	1983	6322720
dna sequence and genome organization of genital human papillomavirus type 6b.	the complete nucleotide sequence of the circular double-stranded dna of the genital human papillomavirus type 6b (hpv6b) comprising 7902 bp was determined and compared with the dna sequences of human papillomavirus type 1a (hpv1a) and bovine papillomavirus type 1 (bpv1). all major open reading frames are located on one dna strand only. their arrangement reveals that the genomic organization of hpv6b is similar to that of hpv1a and bpv1. the putative early region includes two large open reading f ...	1983	6321162
human papillomavirus (hpv) venereal infections and gynecologic cancer.		1983	6326025
the role of keratinocyte differentiation in the expression of epitheliotropic viruses.	we have examined the growth of three epitheliotropic viruses in cultures of human epidermal keratinocytes: herpes simplex virus (hsv) type 1, adenovirus type 2 (ad-2), and human papillomavirus (hpv) type 1. differences were noted in the level of expression of each virus, and these differences may be related to a dependency or lack of dependency on keratinocyte differentiation for complete viral growth. of the three viruses studied, hsv was the only one to replicate productively in all cells of t ...	1983	6190958
introduction of cloned human papillomavirus genomes into mouse cells and expression at the rna level.	the entire dna genomes of five different human papillomaviruses (hpvs) were cloned into the bamhi site of pbr322 (hpv-1a, hpv-3, hpv-4, and hpv-9) or the ecori site of pbr325 (hpv-2), using as starting materials virus preparations isolated from papillomas of individual patients. under stringent hybridization conditions (tm-28 degrees), the five cloned hpvs exhibited less than 10% homology with one another. to establish model cell systems that may be useful for the identification of hpv genes and ...	1983	6193636
histologic, nuclear dna, and human papillomavirus studies of cervical condylomas.	fifty-four cervical condylomatous lesions, with and without nuclear atypia, from 42 women, were studied with the feulgen microspectrophotometric technique for nuclear dna quantitation and an immunoperoxidase technique for human papillomavirus (hpv) antigen. all ordinary cervical condylomas (without atypia/dysplasia) had a diploid or polyploid nuclear dna distribution; 61% had detectable hpv antigen. among the cervical condylomas with atypia/dysplasia, 55% (17/31) had diploid or polyploid nuclear ...	1983	6193864
detection of human papillomavirus type 5 dna in skin cancers of an immunosuppressed renal allograft recipient.	a renal allograft recipient with an epidermodysplasia-verruciformis-like syndrome was found to have human papillomavirus type 5 (hpv-5) in his benign warty lesions and hpv-5 dna in two of his skin cancers. this finding points to a role for hpv-5 in skin oncogenesis in renal allograft recipients.	1983	6135911
morphological and immunohistochemical evidence of human papilloma virus (hpv) involvement in the dysplastic lesions of the uterine cervix.	a series of 110 biopsies of the uterine cervix containing dysplastic and/or in situ changes were assessed morphologiccally with reference to presence or absence of the coexistent condylomatous lesions, and using the immunoperoxidase-pap technique to disclose the hpv (human papilloma virus) antigens in the cells. morphologically, 79 biopsies contained condylomatous (co) lesions (63 flat, 12 inverted and 4 papillomatous ones), and 31 were non-condylomatous (nco) dysplasias/in situ carcinomas. the ...	1983	6141079
characterization of two hpv-3 related papillomaviruses from common warts that are distinct clinically from flat warts or epidermodysplasia verruciformis.	we have recently identified two unusual human papillomavirus (hpv) isolates while engaged in an ongoing study of wart disease in meat handlers and veterinarians. the papillomas from which these two viruses were isolated clinically resembled verruca vulgaris rather than either flat warts or epidermodysplasia verruciformis (ev). these two previously uncharacterized hpvs were molecularly cloned and characterized with respect to known hpvs. the genomes of the two viruses exhibited dramatically diffe ...	1983	6302170
cervical papillomavirus infection in diethylstilbestrol-exposed progeny.	histologic sections from cervical and vaginal biopsies showing dysplasia in 37 women exposed to diethylstilbestrol (des) in utero were stained for human papillomavirus structural antigens using an immunoperoxidase technique. forty-three percent of the lesions had detectable papillomavirus antigens. these findings indicate that a significant proportion of cervical dysplasia observed in des-exposed progeny is associated with papillomavirus infection.	1983	6296741
isoantigen status in condyloma acuminata of the uterine cervix: an immunoperoxidase study.	the immunoperoxidase technic was used to investigate the blood isoantigen status in condyloma acuminata, which is regarded as being caused by the human papillomavirus (hpv). since hpv is associated with epithelial atypias and intra-epithelial neoplasia, and since epithelial malignant transformation is associated with isoantigen loss, the purpose of the study was to determine if koilocytotic atypias are associated with isoantigen loss. complete isoantigen loss was seen in 33% of cases, partial lo ...	1983	6297292
human papilloma virus 5-dna in a carcinoma of an epidermodysplasia verruciformis patient infected with various human papillomavirus types.	an epidermodysplasia verruciformis patient suffering from generalized warts and a carcinoma at the forehead was found to be infected by at least six types and subtypes of human papillomaviruses. the central part of the carcinoma, however, harbored only human papillomavirus 5 dna. the dna persisted extrachromosomally in high genome copy number. in contrast to wart dna preparations, a significant part of the viral sequences in the carcinoma was present as oligomers, at least part of them being con ...	1983	6297727
laryngeal papillomavirus infection during clinical remission.	biopsy samples from 20 patients with a history of laryngeal papillomas were analyzed by southern blot hybridization for the presence of human papillomavirus dna. all papillomas examined contained papillomavirus dna sequences. four samples from uninvolved sites in two patients with active disease and eight samples from patients in remission also contained viral dna. no human papillomavirus dna was detected in a third patient seen during remission. these results explain the clinical pattern of fre ...	1983	6302507
identification of three distinct papillomavirus genomes in a single patient with epidermodysplasia verruciformis.	benign papillomas from a patient with a family history of epidermodysplasia verruciformis were examined for the presence of human papillomavirus (hpv) dna. employing stringent hybridization conditions that allow identification of a single type of hpv and radioactively labeled hpv-5 dna as a probe, we have detected hpv dna exhibiting sequence homology to hpv-5 in these tumors. restriction endonuclease analysis of this hpv dna confirmed its identity as hpv type 5. however, when hybridization was p ...	1983	6300202
atypical immature metaplasia (aim). a subset of human papilloma virus infection of the cervix.	atypical immature squamous metaplasia (aim) is a poorly understood lesion with uncertain biological and clinical significance. this report reviews 170 cases of cervical condylomata and 60 cases of high-grade cervical intraepithelial neoplasia (cin ii/iii); aim was found in association with 34% of condylomata and 16% of high-grade cin. thirty-seven cases of aim alone were reviewed and nearly all presented with a cytologic diagnosis of cin i or condyloma and a colposcopic appearance of white epith ...	1983	6303548
human papilloma virus linked to cervical (and other) cancers.		1983	6304359
detection of human papillomavirus dna sequences in conjunctival papilloma.	two histologically proven conjunctival papillomas (one from a 33-month-old boy and the other from a 28-year-old woman) were examined for the presence of papillomavirus by dna molecular hybridization. the first case, a recurrent tumor known to be positive for papillomavirus structural antigen, demonstrated human papillomavirus dna sequences that cross-hybridized to a human papillomavirus type 11 dna probe. the second case, an initial tumor, which was negative for papillomavirus structural antigen ...	1983	6314814
sequences of bovine papillomavirus type 1 dna--functional and evolutionary implications.	a swedish isolate of bovine papillomavirus type 1 (bpv1) was cloned and a 4807 base pairs long sequence was determined, covering the entire late region of the bpv1 genome and strategic parts of the early region. the sequence reveals two large uninterrupted reading frames, designated l1 and l2, which encode tentative polypeptides with molecular weights of 55.5 and 50.0 k. the promoter region for early transcription and the common 3'-end of early transcripts were identified in the sequence by perf ...	1983	6304656
a papillomavirus dna from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions.	dna from one biopsy sample of invasive cancer of the cervix contained sequences hybridizing with human papillomavirus (hpv) type 11 dna only under nonstringent conditions. this dna was molecularly cloned in lambda phage. under stringent conditions of hybridization it cross-hybridized to a minor extent (less than 0.1%) with hpv types 10, 14, and 15 and showed no homology with dna of other human hpv types. we therefore propose to designate it tentatively as hpv 16. hpv 16 dna was used as a probe t ...	1983	6304740
[cervical condyloma and dysplasia: histological and virological correlation apropos of 100 cases].	colposcopy and cytology have pointed at the frequency of cervical condylomas with special consideration to flat types. the association condyloma/dysplasia and the behavior of carcinomas in situ as sexually transmitted diseases, suggest that human papillomavirus (h. p. v.) could play a role in cervical cancer. 100 patients have undergone examination for coloscopy, cytology, histology and virology. dysplastic features were observed in 47 p. 100 and led to classify the lesions in 3 groups according ...	1983	6305251
evidence suggesting human papillomavirus (hpv) etiology for the squamous cell papilloma of the paranasal sinus.	an extensive squamous cell papilloma of the nasal cavity and also filling the entire left maxillary sinus is reported, which showed cytological features identical to the known cytopathic effects of human papillomavirus (hpv). immunoperoxidase staining disclosed a few cells with nuclei positive for hpv antigens. the findings are discussed in the light of other evidence suggesting a possible viral etiology of these lesions, i.e. the high recurrence rate. the present observations together with the ...	1983	6305303
humoral and cell-mediated immunity to human papillomavirus type 1 (hpv-1) in human warts.	the humoral and cell-mediated immune response to human papillomavirus type 1 (hpv-1) has been studied in 162 patients carrying papillomas of various clinical types: deep plantar wart or myrmecia, common wart, flat wart, and anogenital wart. circulating antibodies were detected by immunodiffusion and microcomplement fixation, using purified hpv-1 particles as type-specific antigen. a significant association between myrmecia and anti-hpv-1 antibodies was found (39% of the cases). cell-mediated imm ...	1983	6305382
the pathology of head and neck tumors: papillomas of the upper aerodigestive tracts, part 18.	the human papillomavirus class of dna viruses are more than circumstantially related to oral and airway papillomas. whether they are fully oncogenic, in the malignant sense, without other agents is questionable. recent advances in molecular virology and the use of genus-specific (common) antigen-antibody reactions have identified papillomavirus in laryngeal and oral papillomas. laryngeal and oral papillomas could be precancerous lesions, but they show a low-risk and long-time interval leading to ...	1983	6305881
papillomavirus structural antigens in condyloma acuminatum of the male urethra.	current developments in the technology of viral identification have rekindled interest in the relationship of human papillomaviruses, condylomata acuminata and neoplasia. although important observations are being reported in the female genital system the association of human papillomavirus and mucosal condylomata in male patients has not been well documented. using the horseradish peroxidase technique and antisera to papillomaviruses, viral antigens were identified in 62 per cent of male patient ...	1983	6306287
human papillomavirus (hpv) involvement in squamous cell lesions of the oral cavity.		1983	6306646
immunohistochemical demonstration of human papilloma virus (hpv) antigens in oral squamous cell lesions.	six oral squamous cell tumours classified as focal epithelial hyperplasia (feh), condyloma acuminatum (ca) and squamous cell papilloma (sqp) were subjected to indirect immunoperoxidase staining with anti-human papillomavirus (anti-hpv) antiserum to demonstrate the possible presence of hpv antigens in these lesions. the results are discussed in the light of the observations on hpv-lesions elsewhere in the body (in uterine cervix), and a suggestion is made to adopt the name condyloma for all those ...	1983	6307342
human papillomavirus structural antigens in squamous papillomas of the male urethra.	twenty-five squamous papillomas of the male urethra were examined for the presence of human papillomavirus (hpv) whole (structural) virion antigens by a peroxidase-antiperoxidase technique having immunospecificity against genus-specific (common) antigens of the papillomavirus genus. eleven of 25 (44 per cent) lesions were positive for hpv. the positive reaction was invariably intranuclear in the superficial epithelium. evidence suggests that these papillomas arise by hpv infection of metaplastic ...	1983	6302329
serological evidence for the association of papillomavirus and cervical neoplasia.	serum was collected from patients with anogenital warts, cervical intraepithelial neoplasia (cin), and invasive squamous carcinoma of the uterine cervix. all sera were screened with a solid-phase enzyme-linked immunosorbent assay for an igg antibody to a group-specific papillomavirus antigen. all three study groups had higher antibody levels than did control groups of children and adults. the invasive-cancer group had a higher geometric mean titre than the cin or anogenital wart groups. it is pr ...	1983	6134887
establishment of a human keratinocyte cell line carrying complete human papillomavirus type 1 genomes: lack of vegetative viral dna synthesis upon keratinization.	a human papillomavirus type 1 (hpv-1)/pbr322 recombinant plasmid was constructed consisting of two complete hpv-1 genomes in a head-to-tail arrangement, inserted into the bamhi site of pbr322. to obtain established human keratinocytes keratinocytes carrying dimeric hpv-1, origin-minus simian virus 40 (sv40) dna was used as a dominant transforming marker in co-transfection experiments performed with cultured human foetal keratinocytes. using the southern blotting technique, hpv-1 dna was detected ...	1983	6190988
immunology of human papillomavirus: warts.	great progress has been made over the last five years in our understanding of papillomavirus (pv) biology. new technology has enabled investigators to understand the relationship between the pv and its host. the pv cannot be cultured in vitro, and this has led to limitations for those wishing to study the biology of this virus. however, utilizing recombinant dna technology, investigators now have abundant quantities of human papillomavirus (hpv) dna for study. such hpv genomes may be labeled wit ...	1983	6095237
on the nature of the deep cellular disturbances in human-papilloma-virus infection of the squamous cervical epithelium.	in the squamous epithelium of the cervix, disturbed deep cells, usually regarded as dysplastic, often underlie superficial koilocytes. recognition of koilocytosis as a cytopathic effect of human papilloma virus (hpv) prompts reconsideration of the state of the deep cells, which could be non-dysplastic or dysplastic. in the dysplastic states hpv could be non-causative or causative. for dysplastic causation by hpv, a cell-differentiation hypothesis reconciles the coexistence in the epithelium of a ...	1983	6136697
the relationship between human papillomavirus and lower genital intraepithelial neoplasia in immunosuppressed women.	in a group of 20 immunosuppressed women with lower genital neoplasia, evidence of associated human papillomaviral infection was found in all patients on the basis of the histologic identification of koilocytes in the upper strata of areas of mild or moderate dysplasia. immunohistochemical study of similar areas disclosed human papilloma structural antigens in the lesions in 60%, while 50% had lesions in which human papilloma virions were detected by the electron microscope. an abnormal immunolog ...	1984	6091459
bowenoid dysplasia in human papillomavirus-16 dna positive flat condylomas during interferon-beta treatment.		1984	6145903
[importance and therapy of sexually transmitted diseases (caused by ureaplasma, mycoplasma, streptococci, cytomegalovirus, human papilloma virus)].		1984	6091289
[detection of papillomavirus capsid antigens and human papillomavirus 16-related dna in bowenoid papulosis and bowen disease].	in one out of 22 cases of histologically diagnosed bowenoid papulosis, we could detect human papilloma virus (hpv) capsid antigen in paraffin sections fixed with formalin by means of the peroxidase-antiperoxidase (pap) test; there were no positive findings regarding biopsies taken from bowen's disease. southern blot hybridization technique revealed hpv 16 dna in 8 out of 10 cases of bowenoid papulosis; there was one positive identification out of 5 cases of bowen's disease.	1984	6091356
the koilocyte and cervical intraepithelial neoplasia: time-trend analysis of a recent decade.	to determine whether the role of the human papillomavirus in the development of cervical intraepithelial neoplasia has changed recently, cervical biopsy specimens from the years 1972 and 1982 were reviewed to compare the frequency of diagnosis of human papillomavirus-associated koilocytosis in patients with suspected cervical intraepithelial neoplasia. no significant difference was found in the frequency of koilocytosis in biopsy specimens with cervical intraepithelial neoplasia between the year ...	1984	6091461
[cytologic diagnosis of cervico-vaginal infections with herpesvirus hominis type ii and human papilloma virus].		1984	6091487
molecular cloning and characterization of the genomes of nine newly recognized human papillomavirus types associated with epidermodysplasia verruciformis.	the genomes of 11 human papillomaviruses (hpvs) found in benign lesions of eight patients suffering from epidermodysplasia verruciformis were cloned in escherichia coli after insertion into plasmid pbr322. the study of the sensitivity of the cloned hpv dnas to 14 restriction endonucleases permitted the construction of physical maps. dna-dna hybridization experiments, performed under stringent conditions, showed that these viruses represent nine new types, hpvs 14 (with subtypes a and b), 15, 17 ...	1984	6092701