Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a highly conserved nucleotide string shared by all genomes of human papillomaviruses. | the nucleotide string taaaacgaaagt is the longest perfect homology shared by all sequenced human papillomavirus genomes. this nucleotide string, which was also found to be highly specific for human papillomavirus genomes, shares the same genomic position in all viral types (5' end of the e1 open reading frame) and putatively codes in every case for the same amino acids. one possible evolutionary model was used to estimate the probability of random occurrence of the nucleotide string in 10 human ... | 1991 | 1665615 |
| diagnosis of cervical intraepithelial neoplasia and human papillomavirus infection: punch biopsy versus cervical smear. | in 102 patients referred to our colposcopy clinic because of one to three papanicolaou smears indicating cervical intraepithelial neoplasia (cin) and/or abnormal colposcopy, routine smears and colposcopically directed punch biopsies were taken simultaneously. for detection and typing of human papillomavirus (hpv)-dna in situ hybridization was performed in all biopsies and in 46 of the cervical smears. in cases of dysplastic lesions the number of hpv 16/18 (40.5%) and 31/33 (42.9%) was markedly h ... | 1991 | 1665685 |
| analysis of transforming gene regions of human papillomavirus type 16 in normal cervical smears. | exfoliated cells from the uterine cervix of 102 hungarian women with no cytological abnormality were screened using the polymerase chain reaction (pcr) for human papillomavirus (hpv) type 16 infection. twenty-nine patients with histologically confirmed cervical intraepithelial neoplasia (cin) served as reference cases. pcr was performed with 2 different hpv 16 specific oligonucleotide primer pairs flanking a 300 and a 200 base-pair fragment from the early 6 (e6) and early 7 (e7) genes, position ... | 1991 | 1665686 |
| [the male factor in the incidence and persistence of condyloma of the cervix and intraepithelial cervical neoplasms]. | from 1988 to 1990, prospectively, were studied 113 masculine couples (males), and 113 women, treated for intraepithelial cervical neoplasia, alone, or associated to viral infection by human papilloma virus-80 males were asymptomatic (70.7%). colposcopy and local application of acetic acid, were needed for the diagnosis of 69.9% of lesions, which presented with a gray color to a brilliant white color. papulous lesion, (56.70%) was most frequent; and the classical lesions as condyloma acuminatum, ... | 1991 | 1665833 |
| human papillomavirus infection in vulvar lesions of lichen sclerosus et atrophicus. | human papillomaviruses (hpv) types 6, 11, 16 and 18 infections were investigated in 18 vulvar lesions of lichen sclerosus et atrophicus (lsa) using in situ dna hybridization and polymerase chain reaction (pcr) on formalin-fixed paraffin-embedded specimens. four out of 18 specimens were found to be infected with hpv type 16 using the pcr technique. interestingly, three of these four patients were in a premenopausal state. this difference proved to be the only distinguishing feature in a compariso ... | 1991 | 1666278 |
| presence of human papillomavirus genome in human tumor cell lines and cultured cells. | a series of 47 human carcinoma cell lines and their cultured cells were examined for human papillomavirus (hpv) genomes with the use of an hpv detection kit (dna-rna hybridization, mixed hpv dna probe of types 6, 11, 16, 18, 31, 33 and 35). four of 8 cases of mild dysplasia, 3 of 9 cases of severe dysplasia, 3 of 7 cases of carcinoma in situ, 3 of 15 cases of uterine carcinoma and 5 of 6 cases of condyloma acuminatum were shown to contain the hpv dna genome in primary cultured cells, while hpv w ... | 1991 | 1666288 |
| oral manifestations of recently described viral infections, including aids. | oral viral diseases secondary to human immunodeficiency virus-1 infection are not commonly observed, except for epstein-barr virus infection. for the most part, members of the herpes virus group and the human papilloma viruses are involved. with the introduction of techniques like the polymerase chain reaction, the level of detection has been changed considerably. however, the application of these techniques may also result in difficulties of interpretation, such as is the case with the detectio ... | 1991 | 1666307 |
| oral manifestations of viral infections in immunocompromised patients. | viral infections are a significant cause of morbidity and mortality in immunosuppressed patients. it is clear that diseases or medical treatments that have cytostatic or cytotoxic effects on lymphocytes and disrupt cytokine production or activity increase the risk of viral infections. while the rate of viral infection varies with the nature and degree of immunosuppression, it is clear that reactivation of latent virus is the most important determinant of the types of viral infections most freque ... | 1991 | 1666308 |
| the association between cervical carcinoma and human papilloma virus (hpv) in xiangyuan county. | a mass survey was conducted to investigate the association between cervical carcinoma and human papilloma virus (hpv) infection in a high-risk area, xiangyuan county, shanxi province. fifty-four cases of cervical cancer in situ (cis), including severe dysplasia (cin iii), 14 cases of invasive cervical carcinoma, 28 cases of mild cervical dysplasia (cin i), and 13 cases of moderate cervical dysplasia (cin ii) were identified among 6710 women examined. one hundred and sixty-nine punch biopsies fro ... | 1991 | 1666523 |
| the management of minor degrees of cervical dysplasia associated with the human papilloma virus. | this report attempts to define further the natural history of minor degrees of cervical dysplasia associated with human papilloma virus (hpv). five hundred and twenty-five patients with a diagnosis of mild to moderate cervical dysplasia and hpv effects were followed without treatment for six months to five years. those patients who progressed to a greater degree of dysplasia were removed from follow-up and treated appropriately. those patients who regressed to a non-dysplastic state were returne ... | 1991 | 1667239 |
| use of the polymerase chain reaction to study the relationship between human papillomavirus infections and cervical cancer. | although it is now evident that human papillomaviruses (hpv) are strongly associated with cervical cancer, their etiological role in the oncogenesis of this disease is still unknown. however, hpv screening may identify women at risk of acquiring this disease. with the recent development of the polymerase chain reaction (pcr), it has become possible to detect small numbers of human papillomavirus genomes in clinical samples. the sensitivity and specificity of this technique, together with the pos ... | 1991 | 1667299 |
| ultrastructural visualization of human papillomavirus dna in verrucous and precancerous squamous lesions. | human papillomavirus (hpv) dna was ultrastructurally localized by the non-isotopic in situ hybridization technique in formalin-fixed, paraffin-embedded specimens of verruca vulgaris of the skin, condyloma acuminatum of the penis and severe dysplasia of the uterine cervix. biotinylated dna probe cocktails were employed for the visualization of hpv-dna, types 6 and 11 (hpv 6/11) and types 16 and 18 (hpv 16/18). the papillomavirus genus-specific antigen was also visualized by pre-embedding immunoel ... | 1991 | 1667450 |
| [type distribution of human papilloma virus (hpv) in cervical cancer]. | for analysis of the relationship between hpv type and the progress of cervical cancer, we investigated the distribution of hpv genome type in 171 cases of papilloma, cervical neoplasm early cancer and invasive cancer. the results showed that chinese human papilloma virus type 16 (chpv16) and chinese human papilloma virus non-classified type 1 (chpvx1) have positive rates 11.5%, 4.8%, 50.0% and 48.8%, respectively. while hpv11/hpv6 decreased with developing of cervical neoplasia. their positive r ... | 1991 | 1667495 |
| detection of human papillomavirus types 6, 11, 16 and 18 in mucosal and cutaneous lesions by the multiplex polymerase chain reaction. | a multiplex polymerase chain reaction (pcr) based on the simultaneous amplification of human papillomavirus (hpv) types 6/11, 16 and 18 in a single-step procedure was developed, using primers chosen in the e6-e7 region. the specificity and sensitivity of this technique have been proved by amplifying mixtures or various amounts of plasmid-containing hpv dna; it allowed the detection of as few as 5-25 hpv dna copies. application of the multiplex pcr to 71 clinical samples showed that hpv dna was d ... | 1991 | 1667785 |
| detection of human papillomavirus type 16 e6/e7 transcripts in fixed paraffin-embedded cervical cancers by the polymerase chain reaction. | a simple, and sensitive method for the detection of human papillomavirus (hpv) 16 e6/e7 transcripts in rna purified from formalin acetic alcohol (faa)-fixed, paraffin embedded cervical cancer (cacx) tissue is described. the entire procedure, including polymerase chain reaction (pcr) amplification of cdna obtained from mrna by reverse transcription, can be completed within a day. the results obtained compare favourably with those using total nucleic acids extracted from the same fixed material, a ... | 1991 | 1667790 |
| accidental use of low-dose 5-fluorouracil in pregnancy. | five percent 5-fluorouracil (5-fu), an antineoplastic agent, is often applied to the lower genital tract in the treatment of vaginal and vulvar human papillomavirus infections. little is known regarding possible teratogenic effects from its topical use in pregnancy. five cases of antenatal lower genital tract 5-fu use were correlated with pregnancy outcome. topical 5-fu was applied up to 16 weeks' gestation. intravaginal doses ranged from 1 to 2.5 g; one patient applied 5-fu to the vulva. antena ... | 1991 | 1667807 |
| a comparative study of pap technique and in-situ molecular hybridization for identification of human papilloma viruses in cervical lesions. | the association of human papilloma virus (hpv) with cervical lesions was correlated using peroxidase anti-peroxidase (pap) technique and in-situ molecular hybridization (mh) with h3 labelled dna probes (hpv type 6, 11, 16 and 18). the pap technique to demonstrate capsid antigen and molecular hybridization for dna sequences was studied in 30 cases. the correlation of hpv with cervical lesions was seen in 29.9% cases with pap technique unlike 73.4% using m.h. technique, indicating the efficacy of ... | 1991 | 1667992 |
| [epidemiological aspects of cervical neoplasms]. | a review concerning the epidemiological issues relating to cervical cancer, one of the most frequent in the women of developing countries, was undertaken in brazil, the incidence rate varies from 23.7/100,000 in porto alegre to 83.2/100,000 in recife. in the united states, the 1978 incidence rate was 6.8/100,000 in white women and 14.7/100,000 among black women. several studies have suggested the hypotheses that cervical cancer could be related to some venereal agent. the evidences have shown th ... | 1991 | 1668183 |
| refolding and purification of human papillomavirus type 16 e7-lacz fusion protein expressed in escherichia coli. | human papillomavirus (hpv) type 16 e7-lacz fusion protein was produced in escherichia coli, extracted as inclusion bodies, refolded with reducing reagents, and subjected to gel filtration. the refolded protein was purified by ion-exchange column chromatography, resulting in a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 1h nuclear magnetic resonance spectral changes were observed in the high field methyl region in the presence of zn2+ ion, suggesting that the refolde ... | 1991 | 1668271 |
| the identification of human papillomavirus within sinonasal papillomas. | 1991 | 1668787 | |
| [carcinoma in situ and human papillomavirus infection]. | the paperwork objective is to analyze if the hpv infections are associated to ca in situ (cis). the cytologic, colposcopic and histologic examinations of women affected by cis were revised. it was determined that a group of them were also infected by hpv. on them, it was determined the viral genotype by nucleic acids hybridization. the toki score was also utilized. | 1991 | 1668982 |
| verrucous cyst. | human papillomavirus has been detected in a variety of cutaneous premalignant and malignant conditions, as well as in benign cutaneous warts. changes consistent with epidermis papilloma virus infection occurring in epidermal cysts have recently been reported in the dermatologic literature only in plantar epidermal cysts. we report five examples of a form of cutaneous cyst that is histologically distinctive and that has not been previously reported, to our knowledge. we describe five cases of epi ... | 1991 | 1668997 |
| detection of human papillomavirus dna in squamous cell carcinoma of the nail bed and finger determined by polymerase chain reaction. | increasing evidence supports the association of squamous cell carcinoma of the finger and nail bed with human papillomavirus infection. we treated 12 patients with squamous cell carcinoma of the finger and nail bed, none of whom was originally diagnosed as having a malignant lesion. half of the patients were referred for carbon dioxide laser ablation of what was believed to be a recalcitrant verruca vulgaris. our objective was to evaluate these lesions for the presence of human papillomavirus by ... | 1991 | 1668998 |
| human papillomavirus. | 1991 | 1668999 | |
| viruses and cancer. causal associations. | this review first considered some general problems in establishing causal links between a virus and a human cancer and offered some guidelines in the pursuit of this objective. second, it reviewed the current causal associations for several candidate oncogenic viruses in relation to the tumors with which they are associated. these include epstein-barr virus in relation to burkitt's lymphoma, nasopharyngeal carcinoma, hodgkin's disease, and non-hodgkin's lymphoma; hepatitis b and c viruses in rel ... | 1990 | 1669491 |
| grading colposcopic appearance: paired comparison between two methods for differentiating benign papillomaviral infection from high-grade dysplasia of the uterine cervix. | histologic features of 134 cases of human papilloma virus (hpv) cervical infection and cervical intraepithelial neoplasia (cin) were reviewed and correlated with colposcopic diagnosis on the basis of two different grading methods: international nomenclature for colposcopy showed 79.1% predictive accuracy and reid's improved colposcopic index 86.6% of accurate predictions (significant difference at p less than 0.05). overestimation difference was the most significant, with 23 overestimates in int ... | 1991 | 1671368 |
| human papillomavirus up-date. meeting held in são paulo, brazil, november 8-10, 1990. | 1991 | 1673450 | |
| colposcopic evaluation of human immunodeficiency virus-seropositive women. | to determine the effect of human immunodeficiency virus (hiv) infection on cervical histology, 32 known hiv-seropositive women underwent cervical colposcopic evaluation. all had cervical cytology, colposcopically directed biopsy, and t-cell studies performed. thirteen of 32 patients (41%) had cervical intraepithelial neoplasia (cin). another 14 of 32 patients (44%) had histologic evidence of cervicitis. abnormal cytology, noted in only three women, suggested cin in one and inflammatory atypia in ... | 1991 | 1675456 |
| subclinical human papilloma virus infection in condylomata acuminata patients attending a vd clinic. | in 37 (77%) of 48 patients with external genital warts, application of 5% acetic acid revealed areas of acetowhite epithelium. the lesions were not clinically apparent before acetic acid was applied but were easily detected without the use of a colposcope. in a control group of 20 patients with chlamydial urethritis and no history of genital warts, none had acetowhite genital lesions. histological examination of biopsy specimens from the flat acetowhite lesions showed hpv infection with koilocyt ... | 1991 | 1678233 |
| neoplastic effects of oral contraceptives. | the potential association between the use of oral contraceptives (ocs) and certain types of cancer remains an important concern. epidemiologic studies published over the past decade indicate that the overall risk of breast cancer is not increased among women exposed to ocs. some studies have suggested an increased risk among younger women with long-term use, and this issue requires further study. ocs confer significant protection against endometrial and ovarian cancers, and the effect is duratio ... | 1991 | 1678377 |
| squamous-cell carcinoma of the endometrium and cervix. | we present a case of endometrial squamous-cell carcinoma in a hysterectomy specimen also showing cis of the cervix with signs of human papillomavirus infection in a 64-year-old woman. the hypothetical histogenetic relationship of both neoplasms is discussed taking together the evidence collected in the very few similar cases on record. | 1991 | 1680092 |
| increased age and mortality associated with cervical carcinomas negative for human papillomavirus rna. | attempts to relate presence and type of human papillomavirus in cervical carcinoma with prognosis have yielded conflicting results. to further investigate this relation, the association between survival of cervical cancer patients after diagnosis and the presence of human papillomavirus (hpv) rna within the tumour was assessed retrospectively. formalin-fixed biopsy specimens from 212 patients with cervical carcinoma who had been followed for up to 6 years were tested by in-situ hybridisation wit ... | 1991 | 1681267 |
| detection of human papillomavirus dna in gynaecological swabs by filter in situ hybridization. | gynaecological smears from the endo- and ectocervix of women with and without cytological and colposcopic abnormalities of the epithelium were investigated for human papillomavirus (hpv) types 6, 11, 16, and 18 by filter in situ hybridization (fish). the data were compared with cytological, colposcopic, and histological findings. of the 266 gynaecological smears, hpv dna was detected in 84 (32%); of 101 cytologically and colposcopically hpv negative cases, hpv dna was found in 10%. of 56 women, ... | 1991 | 1683125 |
| colposcopic analysis of genital human papillomavirus infections during an 8-year prospective follow-up. | a long-term prospective follow-up program has been conducted for genital human papillomavirus (hpv) infections in 532 women in kuopio since 1981. by regular examinations (at 6-month intervals) using colposcopy, cytology and/or punch biopsy, 426 of these women have been followed up for the minimum of 48 months (mean 71 +/- 19 months). these 426 women were included in the present report. the colposcopic data of all women at the first and the latest visits were recorded from the colpophotographs, a ... | 1991 | 1684763 |
| comparison of different in situ hybridization techniques for the detection of human papillomavirus dna in cervical smears. | different hybridization methods were used for detection of human papillomavirus (hpv) dna in cervical smears. the results obtained by filter in situ hybridization (fish) are consistent with most of the reports recently published. to overcome the unsatisfactory limitations of this method, especially the difficulties to distinguish clearly between positive and negative signals, we developed an in situ hybridization protocol using a cytospin and 35s-labelled as well as biotinylated dna-probes. for ... | 1991 | 1686958 |
| humoral assays of human sera to disrupted and nondisrupted epitopes of human papillomavirus type 1. | the use of different assay systems and the disparity in results obtained has meant that we have little understanding about the role played by the humoral response during human papillomavirus (hpv) infection. human antibody responses have so far appeared to be largely directed against the major capsid protein, l1. this protein possesses both type-specific and type-common antigenic determinants but it is not known which of these is important in vivo during the natural course of infection. in this ... | 1990 | 1689524 |
| mapping of linear epitopes of human papillomavirus type 16: the l1 and l2 open reading frames. | certain types of human papillomavirus (hpv), notably hpv type 16, are associated with flat or inverted proliferative lesions of the cervix uteri that can progress to malignancy. as a first step towards the serological study of the epidemiology of hpv, we have synthesized the entire amino acid sequences of the 2 major viral capsid proteins of hpv type 16, l1 and l2, as a set of 66 synthetic 20-residue peptides with an overlap of 5 amino acids. the peptides were tested for reactivity with iga, igg ... | 1990 | 1689705 |
| human t cell responses to human papillomavirus type 16 l1 and e6 synthetic peptides: identification of t cell determinants, hla-dr restriction and virus type specificity. | four t cell determinants in the major capsid protein of human papillomavirus (hpv) type 16 l1 and one in the e6 protein associated with cellular transformation were defined using synthetic peptides to stimulate peripheral blood mononuclear cells from asymptomatic individuals. hla-dr restriction was defined using murine l cells transfected with hla-dr genes to present antigen. responses to two of the five determinants by t cell lines and clones were shown to be specific for hpv-16 based on the la ... | 1990 | 1689768 |
| antibodies to human papillomavirus type 16 in patients with cervical neoplasia. | 1990 | 1689789 | |
| detection of human papillomavirus dna in prostate gland tissue by using the polymerase chain reaction amplification assay. | human papillomavirus (hpv) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. of the sexually transmitted types, hpv type 16 (hpv 16) and hpv 18 are commonly associated with severe dysplasia and carcinoma of the uterine cervix. in men, genital hpv infections which have been studied are manifest as external lesions usually involving types other than 16 and 18. the nature of hpv 16 and 18 infections in men has not been explored. since the most common n ... | 1990 | 1691205 |
| in vitro differentiation of epithelial cells from cervical neoplasias resembles in vivo lesions. | cell lines derived from cervical neoplasias, as well as cells from normal cervix and neonatal foreskin were examined in an in vitro culture system (raft system) that allows for stratification and differentiation of epithelial cells at an air-liquid interface. epithelial cells from human foreskin and ectocervix were observed to differentiate in a manner histologically similar to normal epithelium in vivo as indicated by a single layer of basal cells with a defined mid and upper zone. in contrast, ... | 1990 | 1691480 |
| a novel monoclonal antibody against squamous cell carcinoma. | the monoclonal antibody, ins-2, was raised against rat fibroblasts transformed by open reading frames e6 and e7 of human papillomavirus (hpv) dna. in immunoperoxidase testing of frozen sections, the ins-2 antibody was reactive with all squamous cell carcinomas of the uterine cervix and esophagus tested. in contrast, no antibody binding was detected with adenocarcinomas of various origins. similarly, normal tissues, lymphoid cells and erythrocytes from multiple donors were negative, except that b ... | 1990 | 1692016 |
| human cervical and foreskin epithelial cells immortalized by human papillomavirus dnas exhibit dysplastic differentiation in vivo. | human papillomavirus (hpv) dnas are detected in approximately 90% of anogenital carcinomas. to assess directly the effect of hpv on squamous differentiation, normal human cervical and foreskin epithelial cells and cells immortalized by recombinant hpv dnas were transplanted beneath a skin-muscle flap in athymic mice. xenografts containing normal cells formed well-differentiated stratified squamous epithelia 2 to 3 weeks after transplantation, but cell lines immortalized by four hpv types (hpv16, ... | 1990 | 1692766 |
| growth-regulating functions of human papillomavirus early gene products in cervical cancer cells acting dominant over enhanced epidermal growth factor receptor expression. | squamous cell carcinomas of the human anogenital tract are usually associated with infection of specific types of human papillomaviruses (hpv 16, 18, 31, 33, 35). the intracellular concentration of human papillomavirus early gene products e6 and e7 has been directly linked to the proliferative capacity of cervical cancer cells. since the expression rate of epidermal growth factor receptor correlates to growth properties in squamous carcinoma cell lines, it has been presumed that human papillomav ... | 1990 | 1692767 |
| oral mucosal changes in women with genital hpv infection. | sixty different types of human papillomavirus (hpv) are currently recognized. of these, hpv types 1, 2, 4, 6, 7, 11, 13, 16, 18, 30, 32 and 57 have been identified in oral squamous cell lesions. the prevalence and incidence of clinical hpv infections of oral mucosa are incompletely established, and the figures on subclinical and latent infections are completely lacking. similarly, no data exist on transmission of oral hpv infections. a long-term prospective follow-up study was started to assess ... | 1990 | 1692879 |
| expression of cytokeratin no. 19 polypeptide in genital papillomavirus lesions. | a series of 23 punch biopsies proved to contain human papillomavirus (hpv) type 16 and with established clinical course (including hpv-ncin, hpv-cin i, and hpv-cin ii lesion), and 18 additional biopsies of hpv 6-, 11-, 16- or 18-induced genital lesions were analyzed immunohistochemically for expression of cytokeratin no. 19 polypeptide. an immunoperoxidase-abc technique was used with a polyclonal antibody raised against a synthetic nonapeptide corresponding to the residues 2-10 of the nh2-end, n ... | 1990 | 1693587 |
| identification of b epitopes in human papillomavirus type 16 e7 open reading frame protein. | human papillomavirus (hpv) type 16 is implicated in the aetiology of anogenital dysplasia which may progress to malignancy. hpv-16 dna is actively transcribed in cervical carcinomas, the most abundant transcripts being from the e6 and e7 early open reading frames. the e7 protein has been shown to have transforming activity in vitro. in this report we define four immunodominant b epitopes within the protein corresponding to the e7 gene, using a panel of murine monoclonal antibodies. three epitope ... | 1990 | 1693666 |
| antibody-mediated neutralization in vivo of infectious papillomaviruses. | specific antibody-mediated neutralization of infectious human papillomavirus type 11 (hpv-11) was achieved in the athymic mouse xenograft system, in which hpv-11 induced morphological transformation of human foreskin. virus-specific neutralization was demonstrated by the ability of an hpv-11-specific polyclonal antiserum to neutralize hpv-11 infectivity and not bovine papillomavirus type 1 (bpv-1) or cottontail rabbit papillomavirus (crpv) infectivity. in all three virus infectivity systems, neu ... | 1990 | 1693698 |
| immortalization of primary rat embryo cells by human papillomavirus type 11 dna is enhanced upon cotransfer of ras. | transfer of human papillomavirus type 11 (hpv11) dna and a neo(r) marker into primary rat embryo cells (rec) led to colony formation in g418-selective medium. about 20% of hpv11 clones were eventually established in culture but displayed low growth rates. cotransfection of hpv11 dna and an activated ras oncogene led to formation of both drug-resistant flat colonies and phenotypically transformed clones which grew efficiently when expanded in culture. a number of transformants reverted to a flat, ... | 1990 | 1695409 |
| analysis of human papillomavirus type 16 e6-e7 transcription in cervical carcinomas and normal cervical epithelium using the polymerase chain reaction. | cervical biopsies were collected from birmingham women having cervical intraepithelial neoplasia or invasive cervical carcinoma and normal controls, and examined for the presence of human papillomavirus type 16 (hpv-16) e6-e7 dna and mrna using an adaptation of the polymerase chain reaction. hpv-16 e6-e7 sequences were detected in all abnormal biopsies and in 90% of the normal biopsies examined, confirming previous studies describing the high prevalence of cervical hpv-16 infection. while we wer ... | 1990 | 1695672 |
| selective suppression of human papilloma virus type 18 mrna level in hela cells by interferon. | we have studied the effect of ifn on the transcription of integrated hpv 18 dna in hela cells. we found that the amount of cytoplasmic hpv 18 mrna was markedly reduced in hela cells treated with 10(3) iu/ml of ifn alpha or gamma, and that the inhibitory effect was dose dependent and was detectable by 12h after the addition of ifn. furthermore, northern blot analysis indicated that ifn remarkably reduced 3.4 kb mrna species of hpv 18 transcripts in hela cells. in contrast, the level of beta-actin ... | 1990 | 1696477 |
| management of genital infection caused by human papillomavirus. | recent studies of genital wart therapy have evaluated laser surgery, interferon, and 5-fluorouracil. on the basis of considerations of efficacy, cost, adverse effects, and recurrence rates, cryotherapy with liquid nitrogen remains the recommended therapy for most sexually transmitted warts. none of the treatment methods eradicates the papillomavirus, and genital wart recurrences are seen with each method. the influence of treatment of genital warts on the natural course and sequelae of genital p ... | 1990 | 1696748 |
| [genic localization of an epitope of hpv 16 e7 orf]. | 1990 | 1697215 | |
| detection of papillomavirus dna in human prostatic tissue by southern blot analysis. | human papillomavirus deoxyribonucleic acid was detected in prostate tissue from patients with benign prostatic hyperplasia or prostatic carcinoma. radiolabelled genomic probes, specific for the sexually transmitted human papillomavirus types 16 and 18, were used to detect viral genomic sequences in prostate dna samples analyzed by the southern blot technique. viral sequences were identified in dna from 7 of 16 prostate samples including both hyperplastic and carcinoma tissues and including tissu ... | 1990 | 1697221 |
| abnormal differentiation of human papillomavirus-induced laryngeal papillomas. | we studied the proliferation and differentiation of human laryngeal papillomas, which are benign tumors induced by human papillomaviruses. immunofluorescent stains of tissues for a number of differentiation-specific proteins showed abnormal differentiation. papilloma tissue fragments in vitro showed a slightly decreased fraction of proliferating cells that incorporated tritiated thymidine and a markedly reduced incorporation of tritiated uridine when compared with normal tissue. we propose that ... | 1990 | 1698389 |
| mapping of linear epitopes of human papillomavirus type 16: the e1, e2, e4, e5, e6 and e7 open reading frames. | certain types of human papillomavirus (hpv), especially hpv type 16, are associated with proliferative lesions of the cervix uteri that can progress to malignancy. in order to map the linear epitopes of the hpv-encoded proteins, we have synthesized the predicted amino acid sequences of the open reading frames (orfs) in the early region of hpv 16, as a set of 94 synthetic 20-residue peptides overlapping each other with 5 amino acids. the peptides were tested for reactivity with iga, igg and igm a ... | 1990 | 1698732 |
| identification of b-epitopes in the human papillomavirus 18 e7 open reading frame protein. | a panel of murine mab raised against a ms2 replicase/hpv 18 e7 fusion protein included 23 reactive by elisa with hpv 18 e7 determinants. a total of 19 of the 23 recognized linear epitopes in the n-terminal region of the e7 molecule, while the other four were deduced by binding inhibition assays to recognize conformational determinants in this region. all tested antibodies precipitated a 14-kda peptide doublet that corresponded with the predicted size of the e7 protein, from hela cells, but not f ... | 1990 | 1698872 |
| preventing cervical cancer by treating genital warts in men. why male sex partners need androscopy. | the increased incidence of cancer of the genitourinary and anorectal areas has been directly related to the coexistence of genital human papillomavirus infection. the diagnosis and treatment of condyloma acuminatum in men is important not only to prevent cancer but also to decrease the reservoir of virus that is being transferred to women. screening with androscopy (examination of male genitals with a colposcope after a vinegar solution has been applied to the skin) is indicated for all men who ... | 1990 | 1699215 |
| human papillomavirus type 11dna in papillary squamous cell lung carcinoma. | we report a case of papillary squamous cell carcinoma of the lung developing in relation to a condylomatous papilloma and related to human papillomavirus (hpv) infection. the viral origin of the bronchial papillomatous lesion is strongly suggested by cytological and histological features with marked condylomatous changes. no viral capsid antigen was detected by immunohistochemistry. transmission electron microscopy failed to reveal intranuclear viral-like particles in the papillary part of the c ... | 1990 | 1700538 |
| demonstration of evolutionary differences between conserved antigenic epitopes in the minor nucleocapsid protein of human papillomavirus types 6b, 16 and 18. | we studied human papillomavirus (hpv) minor nucleocapsid protein (l2) by epitope scanning. conserved antigenic epitopes identified by rabbit antiserum to bovine papillomavirus (bpv) were revealed in hpv-6b (amino acids, aa, 196-205); hpv-16 (aa:s 376-85) and hpv-18 (aa:s 221-230). l2 proteins. the first two epitopes were situated in hydrophilic regions of the proteins. aligning the aa-sequences that corresponded to the epitopes with the total l2 sequences of bpv and hpv1a revealed consensus moti ... | 1990 | 1700908 |
| definition of murine t helper cell determinants in the major capsid protein of human papillomavirus type 16. | three murine major histocompatibility complex (mhc) class ii-restricted t cell determinants were identified in the major capsid protein l1 of human papillomavirus (hpv) type 16. peptides derived from hpv-16 l1, which contain putative t cell epitopes located by a predictive algorithm, were synthesized and tested for lymphoproliferative activity by direct immunization, followed by in vitro assay of responses to peptides or recombinant hpv-16 l1. the mhc restriction of the stimulatory peptides was ... | 1990 | 1701481 |
| identification of seroreactive regions of the human papillomavirus type 16 protein e4, e6, e7 and l1. | small fragments of the dna of human papillomavirus type 16 (hpv-16) were randomly cloned into the bacteriophage fd which expresses the resulting peptides as part of its capsid. antisera raised against different hpv-16 fusion proteins were used for screening of the phage clones and the reacting peptides were determined by sequencing the inserted hpv-16 dna fragments of the positive recombinants. seroreactive regions of the proteins derived from the e4, e6, e7 (two regions) and l1 (three regions) ... | 1990 | 1701482 |
| rat primary embryo fibroblast cells suppress transformation by the e6 and e7 genes of human papillomavirus type 16 in somatic hybrid cells. | the e6 and e7 genes of human papillomavirus type 16 (hpv-16) transform established lines of rat cells but not rat cells in primary culture irrespective of the expression of the two genes. the reason for this difference between the susceptibilities of cell lines and primary cells was examined by using hybrid cells obtained by somatic cell fusion of rat cell lines transformed by the e6 and e7 genes of hpv-16 and freshly isolated rat embryo fibroblast cells. in these hybrid cells, transformed pheno ... | 1991 | 1702162 |
| therapy of persistent human papillomavirus disease with two different interferon species. | consensus interferon and interferon alfa-2a were used to treat patients with persistent human papillomavirus disease. thirty-one patients were treated with either consensus interferon or a placebo (25 received active drug and 6 received placebo), and 24 patients were treated with interferon alfa-2a. of the 25 patients who received consensus interferon, 19 (76%) showed either complete or partial clearing of condyloma 10 and 16 weeks after initiation of therapy; in 22 of the 24 patients (91%) who ... | 1991 | 1702582 |
| [clinical study of penile cancer]. | recent progress in molecular biology suggests a certain relationship between penile cancer and human papilloma virus infection. however, the treatment for penile cancer has not been established since each institute has experienced only a small number of patients. we analyzed the result of our treatment for 14 penile cancer patients during past twenty years. ten of the 14 patients were initially treated for the primary lesion with a combination of peplomycin (pep) or bleomycin (blm) and radiation ... | 1990 | 1703253 |
| human papillomavirus in prostatic cancer: no evidence found by in situ dna hybridization. | human papillomavirus has been associated with benign squamous tumors, intraepithelial neoplasia, and invasive squamous cancer. the role of human papillomavirus as the most likely precursor of cervical dysplasia is well studied. we know of no available information as to the possible role of human papillomavirus in prostatic hyperplasia and cancer. we studied formalin-fixed paraffin-embedded tissues of 20 cases of glandular hyperplasia and 20 cases of prostatic cancer by in situ dna hybridization ... | 1991 | 1703667 |
| a general procedure for evaluation of immunological relevance of synthetic peptides: peptides synthesized on paper in enzyme-linked immunosorbent assay. | multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (whatman 3mm paper). paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. the method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of hiv, the epstein-barr virus-determined nucl ... | 1990 | 1703730 |
| characterization of human antibody-reactive epitopes encoded by human papillomavirus types 16 and 18. | we have previously reported that the most common human serum immunoglobulin g antibody reactivities to human papillomavirus type 16 and type 18 (hpv16 and hpv18)-encoded proteins are directed against the minor capsid proteins (hpv16 l2 and hpv18 l2) and to the e7 protein of hpv16 (s. a. jenison, x.-p. yu, j. m. valentine, l. a. koutsky, a. e. christiansen, a. m. beckmann, and d. a. galloway, j. infect. dis. 162:60-69, 1990). in this study, the antibody-reactive segments of the hpv16 e7, hpv16 l2 ... | 1991 | 1704924 |
| a method for detection of human papillomavirus dna in stored slides of stained cervical smears. | a simple method was developed for extraction of dna from stored slides of papanicolaou stained cervical smears. hpv type 16 dna was detected by dna hybridization in 7/10 samples from known hpv-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material. | 1990 | 1705024 |
| presence of antibody reactive with synthetic peptide derived from l2 open reading frame of human papillomavirus types 6b and 11 in human sera. | nine oligopeptides corresponding to segments of different open reading frame (orf) proteins of human papillomavirus (hpv) 6b and hpv-16 were prepared and tested for reactivity with human sera in enzyme-immunoassay (elisa). of these only heptadecapeptide derived from l2 orf of hpv-6b, and encoded also by l2 orf of hpv 11, was reactive with some human sera. over 400 human sera of different origin were tested for the presence of antibody to this antigen. while less than 15% of sera from healthy sub ... | 1990 | 1705748 |
| [demonstration of dna tumor viruses in carcinomas of the upper respiratory tract]. | in situ hybridization (ish) using 35s-labeled probes was applied to the detection of viral dnas in 115 epithelial tumours from various sites. epstein-barr virus (ebv) dna was detected in 83% of undifferentiated nasopharyngeal carcinomas (npc) while none of seven squamous cell npc displayed an ebv-specific autoradiographic signal. also, all other tumours investigated, including thymomas, thymic carcinomas, tonsillar carcinomas and medullary breast carcinomas were negative upon ish to ebv-specific ... | 1990 | 1708623 |
| two immunodominant regions of the human papillomavirus type 16 e7 protein are masked in the nuclei of monkey cos-1 cells. | the human papillomavirus type 16 e7 protein is a 98-amino acid (aa)-long nuclear oncoprotein. we established eight independent mouse hybridoma cell lines producing monoclonal antibodies (mabs) specific for the e7 protein and characterized the mabs by competitive binding analysis with the bacterially expressed, nonfusion e7 protein and synthesized oligopeptides. the mabs were classified into two groups; three and five mabs recognizing epitopes in probable immunodominant regions aas 8 to 22 (regio ... | 1991 | 1708934 |
| extinction of the hpv18 upstream regulatory region in cervical carcinoma cells after fusion with non-tumorigenic human keratinocytes under non-selective conditions. | 'universal fuser' clones of a human papillomavirus type 16 positive cervical carcinoma cell line (siha) were established to study the effect of a non-tumorigenic fusion partner on the regulation of a stably integrated chloramphenicol acetyltransferase (cat) gene controlled by the hpv18 upstream regulatory region under non-selective conditions. the cat expressing cells were fused with both non-tumorigenic, spontaneously immortalized human keratinocytes (hacat) and non-modified siha cells. the res ... | 1991 | 1709093 |
| office therapy for human papillomavirus infection in nongenital sites. | the many different treatment possibilities for the eradication of warts provide evidence that no single method that is completely effective has been found. although the various methods described herein are usually successful therapies for warts, they are all associated with treatment failures and side effects. until the perfect cure for warts is discovered, the physician must evaluate every wart carefully before deciding on a course of action. | 1991 | 1711941 |
| interferon treatment of anogenital human papillomavirus-related diseases. | because of their antiviral, antiproliferative, and immunoregulatory properties, ifns are potentially well suited for use in the management of benign hpv-related anogenital diseases. parenteral and intralesional therapy of condylomata with various natural and recombinant ifn preparations has consistently resulted in beneficial response rates ranging between 40% and 60%, often in patients in whom other therapeutic measures have repeatedly failed. adverse effects from ifn are dose dependent and gen ... | 1991 | 1711942 |
| a "public" t-helper epitope of the e7 transforming protein of human papillomavirus 16 provides cognate help for several e7 b-cell epitopes from cervical cancer-associated human papillomavirus genotypes. | we have identified a major t-cell epitope, amino acids 48-54 (drahyni, in one-letter code) in the e7 open reading frame protein of human papillomavirus (hpv) type 16. lymph node cells from mice immunized with synthetic peptides containing drahyni proliferated and produced interleukin when challenged in vitro with peptide or whole hpv-16 e7 fusion protein. the t epitope was recognized in association with all five major histocompatibility complex class ii i-a and i-e alleles tested. synthetic pept ... | 1991 | 1712110 |
| immortalization by human papillomavirus type 16 alters retinoid regulation of human ectocervical epithelial cell differentiation. | human cervical cells are a primary site of papillomavirus infection and 90% of all cervical tumors are positive for human papillomavirus (hpv) dna. over one-half million cases of hpv-associated cervical, vulvar, and penile cancers are reported per year. yet, in spite of the magnitude of this problem, the effects of hpv infection on cervical cell growth and differentiation are not well characterized. to study these effects we have developed a clonal cell line of hpv-16-immortalized ectocervical e ... | 1991 | 1713124 |
| identification of t- and b-cell epitopes of the e7 protein of human papillomavirus type 16. | there is strong evidence implicating human papillomavirus type 16 (hpv16) in the genesis of human genital cancer. viral dna has been identified in invasive carcinoma of the uterine cervix and in cell lines derived from cervical carcinomas. these sequences are actively transcribed, and translation products corresponding to the early (e)-region genes have been identified. the most abundant viral protein is the e7 protein, which has been shown to possess transforming activity for both established a ... | 1991 | 1714516 |
| type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins. | genital human papillomavirus (hpv) 16 infection is frequently associated with cancer of the uterine cervix, as well as with precancerous lesions. in order to generate serologic reagents which might be useful in the diagnosis of hpv 16 infection, rabbit polyclonal and mouse monoclonal antisera were raised to carboxy terminal peptides from the hpv 16 l1 and l2 open reading frames (orfs). anti-l1 and -l2 peptide sera recognized hpv 16 l1 and l2 fusion proteins in western blots and by immunoprecipit ... | 1991 | 1714667 |
| expression of beta 4 integrins in human skin: comparison of epidermal distribution with beta 1-integrin epitopes, and modulation by calcium and vitamin d3 in cultured keratinocytes. | the expression of beta 4 integrins in adult and fetal human skin as well as in cultured keratinocytes was studied by immunodetection with monoclonal antibodies, and compared with that of beta 1 integrins. the distribution of the beta 1 and beta 4 integrin epitopes was entirely different in the adult epidermis. as reported previously by us (j clin invest 84:1916, 1989), the beta 1 epitopes were present most prominently at lateral cell-cell contact points, whereas staining with the beta 4 antibody ... | 1991 | 1714931 |
| specific interaction between hpv-16 e1-e4 and cytokeratins results in collapse of the epithelial cell intermediate filament network. | the human papillomaviruses (hpv) are associated specifically with epithelial lesions, ranging from benign warts to invasive carcinoma. the virus encodes three late proteins, which are produced only in terminally differentiating keratinocytes, two of which are structural components of the virion. the third, e1-e4, is derived primarily from the e4 open reading frame, which represents a region of maximal divergence between different hpv types. e1-e4 does not seem to be a component of the virus part ... | 1991 | 1715519 |
| a research for the relationship between human papillomavirus and human uterine cervical carcinoma. ii. molecular genetic and ultrastructural study on the transforming activity of recombinant retrovirus containing human papillomavirus type 16 subgenomic sequences. | in order to elucidate the role of hpv-16 in the development of genital cancer, nih3t3 cells were transfected by hpv-16 whole genome and its two early genes, e6-e7. besides ordinary calcium phosphate/dna coprecipitation technique, a newly designed recombinant retrovirus containing the hpv-16 genome or subgenomes was used to infect cells for transfer of the target genes. the transforming activities have been demonstrated to be most efficient when a bioengineering technique of this kind is used. hp ... | 1991 | 1716258 |
| androscopy for anogenital hpv. | strong evidence now links anogenital intraepithelial neoplasia to the transmission of the human papillomavirus (hpv) through sexual intercourse. while there is increasing research on women with this disease, less is known about their male sexual partners. | 1991 | 1716295 |
| expression of cytokine receptors and markers of differentiation in human papillomavirus-infected cervical tissues. | human papillomavirus infection of the uterine cervix is associated with a spectrum of benign, premalignant, and malignant epithelial lesions, a process that appears to require the coordinated effects of secondary cellular and environmental events. we have used flow cytometry and immunohistochemistry to examine the expression of the cellular markers for proliferation (interleukin-1, epidermal growth factor receptor, and transferrin receptor) and the markers of cellular differentiation (filaggrin ... | 1991 | 1716421 |
| characterization of a novel human papillomavirus dna in the cervical carcinoma cell line me180. | the human cervical carcinoma cell line me180 was examined for human papillomavirus (hpv) dna and rna. the integrated dna of a presumably new hpv type showing a relationship closer to hpv39 than to hpv18 was cloned and sequenced. hpv sequences from the e6-e7-e1 region are expressed as poly(a)+ rnas. | 1991 | 1716694 |
| interferon inhibition of neoplastic phenotype in cell lines harbouring human papillomavirus sequences. | the transformed phenotype is markedly affected by interferon (ifn) long term treatment in hela and siha cell lines. two months of continuous exposure to 100 and 1000 international units (iu)/ml of human recombinant alpha ifn causes a drastic reduction of anchorage independent growth in soft-agar. no effect on in vitro growing potential was observed at the same concentrations. this antiproliferative effect in soft-agar was more pronounced in siha than in hela cells and was concentration and time ... | 1991 | 1716817 |
| antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. | even though the retinoblastoma gene is one of the best-studied tumor suppressor genes, little is known about its functional role. like all tumor suppressor gene products, the retinoblastoma protein (prb) is thought to inhibit some aspect of cell proliferation. it also appears to be a cellular target of several dna tumor virus-transforming proteins, such as adenovirus e1a, human papillomavirus e7, or simian virus 40 large t antigen. to help in the analysis of prb, we have prepared a new set of an ... | 1991 | 1717832 |
| human papillomavirus dna in tissue biopsy specimens of vulvar vestibulitis patients treated with interferon. | thirteen women with the diagnosis of vulvar vestibulitis based on clinical symptoms, presence of vestibular tenderness on physical examination, and acetowhite changes of the vulvar vestibule were treated with intradermal injection of alpha-interferon. biopsies of the acetowhite areas were analyzed for human papillomavirus (hpv) dna using polymerase chain reaction amplification and dot blot hybridization. eleven of 13 subjects harbored one of the hpv dna types; six of these were type 16 and/or 18 ... | 1991 | 1717908 |
| antigenic and immunogenic epitopes shared by human papillomavirus type 16 and bovine, canine, and avian papillomaviruses. | all types of papillomaviruses (pv) share common, so-called group-specific epitopes. to identify the major group-specific epitopes, we immunized 26 guinea pigs or rabbits with purified bovine pv type 1 (bpv), canine pv, or avian pv from the common chaffinch. the resulting hyperimmune sera, as well as a commercially available rabbit antiserum to bpv and seven monoclonal antibodies to bpv, were tested in an enzyme-linked immunosorbent assay with a set of 66 overlapping 20-amino-acid peptides repres ... | 1991 | 1719234 |
| nonradioactive rna in situ hybridization detection of human papillomavirus 16-e7 transcripts in squamous cell carcinomas of the uterine cervix using confocal laser scan microscopy. | paraffin-embedded squamous cell carcinomas of the uterine cervix selected for the presence of human papillomavirus (hpv) genotype 16 (n = 19) by polymerase chain reaction, were studied for transcription of the early open reading frame e7 (orf e7). nonradioactive rna in situ hybridization (rish) was performed using in vitro generated biotinylated probes. hybrids were visualized by streptavidin gold and silver enhancement staining in combination with confocal laser scan microscopy. quality of mrna ... | 1991 | 1719818 |
| conization for cin associated with human papillomavirus infection. | we report results of follow-up by pap smears and colposcopy in 116 women treated since 1981 by conization for cervical intraepithelial neoplasia with human papillomavirus infection (hpv-cin). the mean follow-up time was 31.9 (sd 19) months. preoperative diagnosis was hpv-cin ii (with extension into the endocervix) in 11 cases and hpv-cin iii in 103 cases; two diagnostic conizations were performed. the histological examination of the cone biopsies showed complete excision of cin in 109 cases (94% ... | 1991 | 1719944 |
| [analysis of human papillomavirus type 16 e6/e7 mrna in cervical cancers and precancerous lesions by means of the polymerase chain reaction with reverse transcriptase reaction]. | we analyzed hpv-16 e6/e7 mrna in human uterine cervical carcinomas and cervical intraepithelial neoplasias (cins) by polymerase chain reaction (pcr) with reverse transcription (rt-pcr). simultaneously total rna and dna were extracted from 6 cervical carcinomas, 14 cins and 2 normal cervical tissues by the guanidium isothiocyanate/cscl method. hpv-16 dna was detected in 3 cervical carcinomas and 6 cins. hpv-16 e6/e7 transcripts were detected in all hpv-16 dna positive cervical carcinomas and cins ... | 1991 | 1720801 |
| newly established uterine cervical carcinoma cell line with co-amplification of human papillomavirus dna and c-myc gene. | a new human tumor cell line, ncc-c-cx-1 (cx-1), was established from a uterine cervical cancer xenografted in nude mice. this cell line harbored approximately 50 to 100 copies of human papillomavirus (hpv) type 18 dna per haploid genome, and contained about 16-fold-amplified c-myc gene with rearrangement. these genomic alterations found in cx-1 cells were also present in both primary tumor and xenografted tumor. histopathologically, original and xenografted tumors were poorly differentiated canc ... | 1991 | 1721614 |
| evaluation of histologic, morphometric, and immunohistochemical criteria in the differential diagnosis of small cell carcinomas of the cervix with particular reference to human papillomavirus types 16 and 18. | clinicopathologic analyses including immunohistochemical, morphometric, virologic, and dna ploidy studies were performed on seven cases of small cell (undifferentiated) carcinoma (scc) and 13 cases of small cell squamous carcinoma (scsc) of the uterine cervix in an attempt to evaluate which criteria are the most useful in identifying aggressive cervical carcinomas composed of small cells. highly malignant behavior was found to correlate most closely with the histologic pattern of the tumor. diff ... | 1991 | 1722042 |
| human papillomavirus type 18 e6 and e7 antibodies in human sera: increased anti-e7 prevalence in cervical cancer patients. | antibody-reactive regions on the human papillomavirus type 18 (hpv-18) e6 and e7 proteins were identified with rabbit polyclonal anti-fusion protein sera by screening of an fd phage expression library containing subgenomic hpv-18 dna fragments and by testing of overlapping decapeptides representing the e6 and e7 open reading frames. peptides comprising the delineated regions (designated e6/1 to e6/4 and e7/1) were synthesized and used in an enzyme-linked immunosorbent assay (elisa) to detect ant ... | 1991 | 1722219 |
| [treatment of human papillomavirus infection of the uterine cervix with gel interferon]. | treatment of uterine cervix infection caused by human papillomavirus still is an enigma. some drugs have been tested with cure rates between 60 to 70%. most of such agents are substances which produce a strong epithelial desquamation and have shown strictly toxic side effects. the use of interferon in this infection has been studied, but different authors disagree in their therapeutic findings. a randomized, double blind trial was conducted to compare interferon to placebo. preliminary results a ... | 1991 | 1723217 |
| cytokeratin intermediate filament pattern and human papillomavirus type in uterine cervical biopsies with different histological diagnosis. | the cytokeratin pattern and the presence of human papillomavirus (hpv) were analyzed in 53 uterine cervical biopsies. the biopsies were histologically characterized and the diagnosis ranged from normal through dysplasia to carcinoma. the cytokeratins were identified by their immunological reactivity with the monoclonal antibodies ae1 and ae3. the tissue was typed for the presence of hpv types 11, 16 and 18. we have previously shown that there was no correlation between the expression of cytokera ... | 1991 | 1723392 |
| the molecular specificity of linear b-epitopes in the e7 open reading frame protein of human papillomavirus 16 defined by monoclonal antibodies. | human papillomavirus (hpv) 16 is highly associated with premalignant and malignant anogenital epithelial lesions. the transforming function resides within the viral e7 open reading frame protein. we have defined three immunodominant linear b-epitopes in the e7 protein. in the present study, we determine the contribution of individual amino acid residues to antibody binding of these three epitopes using replacement set analysis. in this approach, each epitope residue is substituted in turn by eac ... | 1990 | 1724617 |
| [demonstration and biological significance of viral and cellular oncogenes in uterine carcinomas]. | premalignant and malignant lesions of the cervix uteri and endometrium were analyzed for the presence of human papillomavirus (hpv) dna and c-myc or c-erbb2/neu oncogene expression. hpv dna was detected by pcr in 100% of the cervical carcinomas and cin lesions, but also in endometrial lesions (3/8 adenocarcinomas, two hyperplasias and one adenomyosis uteri). myc-overexpression was found in 25-30% of the cervical carcinomas and severe dysplasias, but not in endometrial lesions. c-erbb2 was overex ... | 1991 | 1724842 |
| demonstration of changes in cytokeratin expression in condylomata accuminata in relation to the presence of human papilloma virus as shown by a combination of immunohistochemistry and in situ hybridization. | human papillomavirus (hpv) can be detected in, and is probably involved in the etiology of, the majority of anogenital neoplasias. infection with the virus induces a number of events in the infected epithelial cells that may lead to the development of benign or malignant tumors. one change that can be detected in the infected cells is in squamous differentiation, which is reflected by the pattern of cytokeratin polypeptide expression. by studying this pattern in relation to the presence of the v ... | 1991 | 1725244 |