Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation of etiological agent of hydropericardium syndrome in chicken embryo liver cell culture and its serological characterization. | the virus causing hydropericardium syndrome was isolated in chicken embryo liver (cel) cell culture from livers obtained from naturally infected broilers. the cytopathic effects characterized by rounding and degeneration of cells were visible 36 hr post infection in first passage. at 4th passage level, the infectivity titre was 5.24 log10 tcid50/ml. in may-grunwald and giemsa stained cells, basophilic intranuclear inclusions ('bird eye' inclusion), typical of aviadenovirus infection, were observ ... | 2003 | 15248478 |
| survey of peafowl (pavo cristatus) for potential pathogens at three michigan zoos. | blood samples collected from 31 free-roaming peafowl from three zoos in michigan were tested serologically. antibody titers were present against avian adenovirus and bordetella avium in 19.3% and 61.3% of the samples, respectively. serum plate agglutination tests were positive for mycoplasma meleagridis and mycoplasma synoviae in 3.2% and 38.7% of the samples, respectively. all birds were seronegative for avian influenza, newcastle disease virus, west nile virus, mycoplasma gallisepticum, salmon ... | 2003 | 15077714 |
| prevalence of infectious diseases in ring-necked pheasant flocks in poland. | the health status of ring-necked pheasants in view of the prevalence of infectious diseases was estimated in polish pheasantries in the years 1997-2000. anatomicopathological, microbiological and serological examinations were carried out on birds derived from 26 pheasantries, including birds randomly selected from 18 flocks and sick or dead birds sent from 8 pheasantries. antibodies specific to the following viruses were detected in serum blood samples: he, ae, ap, reo, ai, adeno group 1, md, nd ... | 2003 | 14510048 |
| detection of egg drop syndrome virus antigen or genome by enzyme-linked immunosorbent assay or polymerase chain reaction. | mouse monoclonal antibodies (mabs) were produced against an indian isolate of egg drop syndrome (eds) virus and characterized. four hybridoma clones were secreting mabs that bound to a 100 kda protein, presumably the hexon protein. these mabs were found to cross-react with two other indian isolates of eds virus and to the reference uk 127 strain. three of these mabs were mapped to the same epitope compared with the other mab (f8), which bound to a different epitope. an antigen-capture enzyme-lin ... | 2003 | 14522711 |
| detection of egg drop syndrome 1976 virus by polymerase chain reaction and study of its persistence in experimentally infected layer birds. | polymerase chain reaction (pcr) assay was developed for the detection of egg drop syndrome 1976 (eds-76) virus in tissues, namely in the uterus, spleen and buffy coat. it was also used to study the persistence of the virus in tissues of experimentally infected layer birds. the pcr assay could detect as little as 10 fg of purified eds-76 viral dna. it also amplified the dna of fowl adenovirus serotypes 4 (fav-4) and 8 (fav-8). the virus persisted in the uterus up to day 21 post infection (p.i.). ... | 2003 | 14658847 |
| preliminary data of adenovirus celo localisation in chicken. | 2003 | 14965138 | |
| development of a multiplex pcr for detection of avian adenovirus, avian reovirus, infectious bursal disease virus, and chicken anemia virus. | a multiplex polymerase chain reaction (mpcr) was developed and optimized for the simultaneous detection and differentiation of avian reovirus (arv), avian adenovirus group i (aav-i), infectious bursal disease virus (ibdv), and chicken anemia virus (cav). four sets of specific oligonucleotide primers were used in this test for arv, aav-i, ibdv, and cav. the mpcr dna products were visualized by gel electrophoresis and consisted of fragments of 365 bp for ibdv, 421 bp for aav-i, 532 bp for arv, and ... | 2004 | 15294316 |
| restoration of p53 tumor-suppressor activity in human tumor cells in vitro and in their xenografts in vivo by recombinant avian adenovirus celo-p53. | human adenovirus (ad) vectors are extensively used as gene transfer vehicles. however, a serious obstacle for the use of these vectors in clinical applications is due to pre-existing immunity to human ads affecting the efficacy of gene transfer. one of the approaches to circumvent host immune response could be the development of vectors based on non-human ads that are able to transduce genes into human cells. in this study, we explored the possibility of using avian ad celo vectors as gene-trans ... | 2004 | 14681700 |
| adenovirus-mediated gene delivery: an overview. | adenoviruses, which were first isolated in the 1950s, have been developed as gene-delivery vehicles, or vectors, since the early 1980s. the adenoviruses constitute the adenoviridae family, which is divided into two genera: the aviadenovirus genus infects only birds, whereas the mastadenovirus genus contains viruses that infect a range of mammalian species. human adenoviruses are classified into six subgroups based on the percentage of guanine and cytosine in the dna molecules and the ability to ... | 2004 | 14970581 |
| the hydropericardium syndrome in poultry--a current scenario. | inclusion-body hepatitis hydropericardium syndrome (ibh-hps) is an important, recently emerged, disease of poultry, particularly of 3- to 6-week-old broiler chicks, characterized by its sudden onset, with high mortality ranging from 20% to 70%, typical hydropericardium and enlarged mottled and friable livers, with intranuclear inclusion bodies in the hepatocytes. the causative agent is a non-enveloped icosahedral fowl adenovirus (fav) serotype 4, belonging to the adenovirus genus of the family a ... | 2004 | 14992243 |
| [the celo-ang recombinant avian adenovirus with human angiogenine gene inducing neovascularization in the anterior tibial muscle of rat]. | the celo recombinant avian adenovirus carrying the gene coding the human angiogenine (ang) synthesis was obtained. expression of the angiogenine gene was shown in the lmh cell culture after infection with the celo-ang virus. the ability of celo recombinant adenoviruses to carry out the delivery and expression of alien genes in muscle cells was demonstrated in experiments with laboratory animals (wistar line rats). the induced neovascularization in rat muscles after the animals were administered ... | 2004 | 15597571 |
| hydropericardium syndrome in japanese quail (coturnix coturnix japonica). | 2004 | 15461367 | |
| comparison of hemagglutination inhibition test and elisa in quantification of antibodies to egg drop syndrome virus. | a single-serum dilution elisa for egg drop syndrome (eds) virus-specific antibodies was developed. in testing 425 chicken sera it was found to have a 93.6% sensitivity and 98.7% specificity relative to a hemagglutination inhibition (hi) test. the correlation coefficient for elisa and hi titers was 0.793. the elisa was efficacious in quantification of both vaccinal and infection antibodies and could routinely be used for screening large numbers of field sera. | 2004 | 15595213 |
| abolition of hcar-dependent cell tropism using fiber knobs of atadenovirus serotypes. | most adenoviral vectors use in gene therapy protocols derive from species c. however, expression of the primary receptor (human coxsackievirus and adenovirus receptor, hcar) for these adv is variable on cancer cells. in vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therapy. the ad fiber protein largely determines viral tropism through interaction with specific receptors. hereto, we constructed a set of hadv5 vectors carrying chimeric fibers with kno ... | 2004 | 15051380 |
| restriction enzyme analysis of indian isolates of egg drop syndrome 1976 virus recovered from chicken, duck and quail. | egg drop syndrome 1976 (eds-76) is caused by a haemagglutinating adenovirus belonging to group iii of the genus aviadenovirus in the family adenoviridae. all isolates are serologically identical, but have been divided into three groups based on restriction endonuclease (re) analysis. in this study the viral dna of various indian eds-76 viral isolates (ceds-a, ceds-b, eds-m, eds-ml, eds-1/ad/86, eds-kc and qeds) obtained from different avian species and different geographical regions were digeste ... | 2004 | 15379439 |
| no evidence for adaptation of current egg drop syndrome 1976 viruses to chickens. | in order to determine whether the current field strains of egg drop syndrome (eds) 1976 viruses adapt to chickens, we compared the growth efficiency of three japanese field strains (pa-1/79, awi/98, gifu/01) in chicken and duck embryo liver cells. the growth efficiency in chicken or duck embryo liver cells was almost similar in these strains. the fiber protein may carry the type-specific antigen and the hemagglutination activity, and hexon protein may contain the subgroup-specific antigenic dete ... | 2004 | 15077820 |
| isolation and epidemiology of falcon adenovirus. | an adenovirus was detected by electron microscopy in tissues from falcons that died during an outbreak of inclusion body hepatitis and enteritis that affected neonatal northern aplomado (falco femoralis septentrionalis) and peregrine (falco peregrinus anatum) falcons. molecular characterization has identified the falcon virus as a new member of the aviadenovirus group (m. schrenzel, j. l. oaks, d. rotstein, g. maalouf, e. snook, c. sandfort, and b. rideout, j. clin. microbiol. 43:3402-3413, 2005 ... | 2005 | 16000467 |
| association of fowl adenovirus serotype 12 with hydropericardium syndrome of poultry in india. | eight fowl adenovirus (fadv) isolates obtained from different geographical regions of india were typed by a virus-neutralization test (vnt) using rabbit antisera against all the 12 serotypes of fadv and by pcr for the hyper-variable region of hexon gene combined with restriction fragment length polymorphism (rflp) analysis using alui and mboi restriction enzymes. it was found that six isolates belonged to fadv-4, one to fadv-12 and one to both of them. this study revealed the involvement of fadv ... | 2005 | 16047743 |
| [can obesity be infectious?]. | currently the presence of obesity is increasing and it has become the basic civilisation illness of our times. up to date no attention has been paid to the possibility of etiology of infectious obesity. recently some publications have appeared whose authors suggest a possibility of an infectious derivation of some forms of obesity. six pathogens causing obesity in animals have been described: canine distemper virus (cdv), avian adenovirus, borna disease virus (bdv), smam-1, human adenovirus ad-3 ... | 2005 | 16541729 |
| nucleotide sequence analysis of the l1 loop variable region of hexon gene of fowl adenovirus 4 isolates from india. | three fowl adenovirus 4 (fav4) isolates from chicken and one from quail, all from tamil nadu, india were analyzed. the l1 loop variable region of hexon gene of these isolates was amplified by pcr and sequenced. the nucleotide sequences (442 bp) and deduced amino acid sequences of the four isolates were compared with those of other isolates of fav4. the nucleotide sequences of the four isolates had a 98% homology with other indian isolates and a 96% homology with belgian and russian isolates. the ... | 2005 | 15929401 |
| characterization of a new species of adenovirus in falcons. | in 1996, a disease outbreak occurred at a captive breeding facility in idaho, causing anorexia, dehydration, and diarrhea or sudden death in 72 of 110 northern aplomado falcons (falco femoralis septentrionalis) from 9 to 35 days of age and in 6 of 102 peregrine falcons (falco peregrinus) from 14 to 25 days of age. sixty-two northern aplomado and six peregrine falcons died. epidemiologic analyses indicated a point source epizootic, horizontal transmission, and increased relative risk associated w ... | 2005 | 16000466 |
| partial characterization of an adenovirus-like virus isolated from broiler chickens with transmissible viral proventriculitis. | transmissible viral proventriculitis (tvp) was experimentally reproduced in specific-pathogen-free chickens using a homogenate of proventricular tissue obtained from tvp-affected commercial broiler chickens. thin-section electron microscopy revealed intranuclear, approximately 70-nanometer (nm), adenovirus-like viruses (adlv) within proventricular lesions. the adlv, designated adlv (r11/3), could not be propagated using various avian and mammalian cell cultures or by inoculation of embryonated c ... | 2005 | 16252486 |
| inclusion body hepatitis as a primary disease in broilers in saskatchewan, canada. | in recent years inclusion body hepatitis (ibh) has emerged as an economically important disease in western canada. historically, infections with infectious bursal disease virus (ibdv) and chicken anemia virus (cav) have been known to suppress the immune system of broilers and make them more susceptible to a secondary disease such as ibh. recently it has been reported that virulent adenoviruses are able to cause ibh as a primary disease in broilers without apparent involvement of ibdv and cav. th ... | 2006 | 17274293 |
| [obesity development associated with viral infections]. | this overview of the significance of viral infections in the development of human obesity is presented within context of the commonly recognized obesity risk factors, including the personal and public health consequences of obesity in various countries. in addition, the results of past and recently published studies on the recognition of six taxonomically different viruses which can undoubtedly be associated with obesity progression in some species of animals are summarized. more attention is fo ... | 2006 | 16641892 |
| falcon adenovirus infection in breeding taita falcons (falco fasciinucha). | four female and 3 male taita falcons (falco fasciinucha) out of a breeding colony of 14 taita falcons (7 pairs) died during the breeding season after showing lethargy and anorexia for 1 to 2 days. all animals were submitted for necropsy. gross lesions in the female falcons were characterized by anemia secondary to marked hemorrhage into the ovary and oviduct, serofibrinous effusion into the cardioabdominal cavity and serosal petechiae. in addition, marked necrotizing splenitis and pulmonary hemo ... | 2006 | 16789719 |
| sequence analysis of the left end of fowl adenovirus genomes. | nucleotide sequence analysis of the left end of the genome of fowl adenoviruses (fadv) representing species group c (fadv-4 and -10), d (fadv-2) and e (fadv-8) were carried out, and the sequence data was compared to those of fadv-1 (fadv-a) and fadv-9 (fadv-d). the viruses were propagated in chicken hepatoma cell line for viral dna isolation. restriction endonuclease analysis was performed followed by hybridization with two dna probes representing the left end of fadv-9. the identified fragments ... | 2006 | 16791424 |
| [the induction of protective immune response in mice vaccinated by recombinant avian adenovirus celo expressing glycoprotein g of the rabies virus]. | the recombinant avian adenovirus celo-gprb expressing glycoprotein g of rabies virus (strain ts-80, arriw&m, pokrov, russia) was used for mice vaccination against rabies. double intramuscular immunization by recombinant celo-gprb adenovirus in a dose 10(9) pfu per mouse caused the induction of virus neutralizing antibodies (vna) synthesis in 78% of mice, while twice repeated intradermal injections of the recombinant adenovirus failed to induce the vna production. the protection level in groups o ... | 2006 | 16941876 |
| could a virus contribute to weight gain? | obesity is a serious public health problem associated with increased morbidity and mortality. although the causes for obesity are unclear, it seems that environmental, genetic, neural and endocrine factors contribute to its development. however, the rapid global spread of obesity resembles epidemiologically the spread of an infectious disease. thus far, little consideration has been given to the possibility that the epidemic of obesity could be due to an infectious agent. seven viruses and a scr ... | 2007 | 17420782 |
| genetic characterization, pathogenicity, and protection studies with an avian adenovirus isolate associated with inclusion body hepatitis. | an avian adenovirus (aav) was isolated from liver samples of two 2-wk-old broiler-breeder flocks obtained from grandparents vaccinated at 10 and 17 wks of age with an autogenous inactivated vaccine containing the european aav 8 (8565 strain) and 11 (1047 strain) serotypes (aav8/11 vaccine). affected broiler-breeders exhibited clinical signs and macroscopic and microscopic lesions associated with inclusion body hepatitis (ibh). the isolated adenovirus, identified as stanford, was molecularly char ... | 2007 | 17461263 |
| putative host cell receptor for fowl adenovirus detected in gizzard. | this work was done to identify a fowl adenovirus (fav) binding protein in the gizzard, a known target organ for certain strains of fav serotype 1. by using a virus overlay protein binding assay (vopba), a putative fav binding protein of approximately 200 kda expressed in the gizzard was detected. | 2007 | 18057841 |
| experimental reproduction of transmissible viral proventriculitis by infection of chickens with a novel adenovirus-like virus (isolate r11/3). | transmissible viral proventriculitis (tvp) was experimentally reproduced in 2-wk-old specific-pathogen-free chickens and commercial broiler chickens by eyedrop inoculation of adenovirus-like virus (adlv), isolate r1 1/3. no clinical signs and no weight gain depression were observed in chickens inoculated with adlv (r11/3); however, gross and microscopic lesions characteristic of tvp were present in proventriculi of inoculated chickens. proventriculi of adlv (r11/3)-inoculated chickens were marke ... | 2007 | 17461268 |
| fta liver impressions as dna template for detecting and genotyping fowl adenovirus. | the feasibility of using liver impressions on flinders technology associates (fta filter paper for the collection, inactivation, and molecular analysis of fowl adenovirus (fav) was evaluated. fav i european union (eu) serotype 1 spotted on fta was shown to be inactivated using specific-pathogen-free (spf) primary chicken embryo liver cell culture as indicated by absence of cytopathic effect. sensitivity of the polymerase chain reaction (pcr) test using tenfold dilutions of allantoic fluid from 1 ... | 2007 | 17461276 |
| effects of polyether ionophores on the protective immune responses of broiler chickens against angara disease and newcastle disease viruses. | immunization against angara disease virus (adv), a serotype 4 avian adenovirus, and newcastle disease virus (ndv), an avian paramyxovirus serotype 1, is the mainstay of a broiler vaccination programme, while polyether ionophores usually form an essential component of a broiler medication programme in most parts of india and pakistan. the role of polyether ionophores in the protective immune responses of broiler chickens vaccinated and challenged with adv and ndv was investigated. a total of 1600 ... | 2007 | 17310330 |
| falcon adenovirus in an american kestrel (falco sparverius). | a fatal adenovirus infection is described in a wild-caught american kestrel (falco sparverius). predominate lesions were a moderate to severe hepatitis with diffuse single-cell necrosis of hepatocytes and a splenitis characterized by necrosis of cells surrounding the sheathed arteries. pan-nuclear eosinophilic to magenta inclusion bodies were abundant within hepatocytes. polymerase chain reaction was used to amplify a portion of the hexon gene from dna extracted from the bird's liver and spleen. ... | 2007 | 18065135 |
| serologic monitoring of a broiler breeder flock previously affected by inclusion body hepatitis and testing of the progeny for vertical transmission of fowl adenoviruses. | the increasing number of clinical cases of inclusion body hepatitis (ibh) associated with fowl adenoviruses (fadvs) is a growing concern in different parts of the world, including canada. after an outbreak of ibh in a 10-d-old pullet broiler breeder flock, we serologically monitored the flock from 8 to 46 wk of age, using the agar gel precipitation test (agpt) offered by diagnostic laboratories and an fadv group-specific enzyme-linked immunosorbemt assay (elisa) developed earlier. in addition, w ... | 2007 | 17479772 |
| viruses as an etiology of obesity. | obesity is a serious chronic disease that has numerous etiologies. the prevalence of obesity has increased dramatically since about 1980 in the united states and worldwide in both developed and developing countries. this rapid spread is compatible with an infectious origin. this review discusses the 5 animal viruses and 3 human viruses that have been shown to cause obesity and examines the evidence to date for virus-induced obesity. the obesogenic animal viruses include canine distemper virus, r ... | 2007 | 17908526 |
| periodic monitoring of commercial turkeys for enteric viruses indicates continuous presence of astrovirus and rotavirus on the farms. | a longitudinal survey to detect enteric viruses in intestinal contents collected from turkeys in eight commercial operations and one research facility was performed using molecular detection methods. intestinal contents were collected from turkeys prior to placement, with each flock resampled at 2, 4, 6, 8, 10, and 12 wk of age. the samples were screened for astrovirus, rotavirus, reovirus, and turkey coronavirus (tcov) by a reverse transcriptase and polymerase chain reaction (rt-pcr), and for g ... | 2007 | 17992925 |
| genotyping of canadian isolates of fowl adenoviruses. | five hundred and seventy-three clinical submissions with fowl adenovirus (fadv) involvement were examined to investigate the association of different types of fadv with clinical problems related to fadv infection. samples were received from 2000 to 2006 and originated from seven canadian provinces. four hundred and eighty-seven submissions were inclusion body hepatitis (ibh) related, while 86 were not ibh related. viruses isolated from 287 samples were further analysed by hexon gene loop 1 seque ... | 2008 | 18202956 |
| electron microscopic identification of viruses associated with poult enteritis in turkeys grown in california 1993-2003. | poult enteritis (pe) is one of the most common diseases seen in young turkey flocks. since 1993, more than 1800 cases of suspected pe have been submitted for examination by negative stain electron microscopy; this has involved more than 2400 individual results, because in many cases more than one virus was identified; at least 1500 individual results were positive for viruses. viruses have been identified in poults as young as 3 days and up to 9 wk of age. the most commonly found viruses are rot ... | 2008 | 18646448 |
| [influence of integrin-binding motif (rgd) on attachment and internalization of avian adenovirus celo in mammalian cells]. | recombinant avian adenovirus celo bearing sequence rgd in the structure of a hi-loop of long fiber was designed. experiments in vitro revealed that introduction of rgd-motif into fiber of celo increased the ability of the virus to be attached to a surface of car-negative cells, and raised efficiency of the process of internalization of the virus both in car-positive, and in car-negative cells. | 2008 | 18488447 |
| structure of the c-terminal head domain of the fowl adenovirus type 1 short fibre. | there are more than 100 known adenovirus serotypes, including 50 human serotypes. they can infect all 5 major vertebrate classes but only aviadenovirus infecting birds and mastadenovirus infecting mammals have been well studied. celo (chicken embryo lethal orphan) adenovirus is responsible for mild respiratory pathologies in birds. most studies on celo virus have focussed on its genome sequence and organisation whereas the structural work on celo proteins has only recently started. contrary to m ... | 2008 | 18561970 |
| adenovirus group ii-like infection in chukar partridges (alectoris chukar). | seven live 5-to-6-wk-old chukar partridges (alectoris chukar) were examined because of increased lacrimation, swollen eyelids, and increased mortality. gross lesions consisted of mildly enlarged and mottled white spleens, swollen eyelids with external scab formation, and watery intestinal contents. microscopically, there were increased numbers of mononuclear phagocytic system cells in the spleen, some of which contained faintly staining basophilic intranuclear inclusion bodies, blepharoconjuncti ... | 2008 | 18646470 |
| [production of human recombinant beta-interferon in the avian cell culture]. | the avian recombinant adenovirus of serotype 1 (celo) was obtained. the recombinant adenovirus of serotype 1 (celo) induces expression of human beta-interferon (ib). the expression cassette containing ib gene was placed at the right end of the celo genome under control of hybrid promoter hef-1alpha/htlv. the resulting recombinant adenovirus celo-ib transduced the avian cell culture lmh. the level of production of the recombinant ib was 0.15 micro/ml. the ib protein yield after affine chromatogra ... | 2008 | 18756823 |
| [construction of the vector based on the celo avian adenovirus genome providing enhanced expression of secreted alkaline phosphatase gene in a non-permissive system in vitro and in vivo]. | aviadenovirus celo is a promising vector for gene delivery to eukaryotic cells, including mammalian cells. in the present state of affairs, it gives a chance to apply recombinant adenoviruses celo against animal pathogens. high expression level of transgene consisting of virus vector is necessary to reach protective humoral and t-cell immune answer. one of the approaches to enhance the expression level of transgene consisting of viral vectors is inclusion of addition 5'- and 3'- untranslated reg ... | 2008 | 19172876 |
| sequence comparison of the right end of fowl adenovirus genomes. | the nucleotide sequence at the right end of the genomes of fowl adenoviruses (fadvs) representing species groups c (fadv-4 ca and fadv-10 c-2b), d (fadv-2 ca), and e (fadv-8 ca) was determined and analyzed and compared to fadv-1 (species group a virus) and fadv-9 a-2a (species group b virus). high nucleotide sequence identities and amino acid identities (74-94%) were found among viruses in the same species group. homologues to known open reading frames (orfs) of the right end of the reported fad ... | 2008 | 18204967 |
| adenoviral gizzard erosions in italian chicken flocks. | 2009 | 19525525 | |
| production of recombinant human lactoferrin in the allantoic fluid of embryonated chicken eggs and its characteristics. | the human iron-binding protein lactoferrin (hlf) has been implicated in a number of important physiological pathways, including those regulating immune function and tumor growth. in an effort to develop an efficient system for production of recombinant hlf (rhlf) that is structurally and functionally equivalent to the natural protein, we generated a recombinant celo (chicken embryo lethal orphan) avian adenovirus containing an expression cassette for hlf. embryonated chicken eggs were infected w ... | 2009 | 19174190 |
| detection and quantitation of fowl adenovirus genome by a real-time pcr assay. | the purpose of the study was to develop a highly sensitive real-time polymerase chain reaction (pcr) assay to detect and quantitate fowl adenovirus (fadv) dna in chicken tissues, using fadv-9 as a model. the assay had a dynamic range of 7 logs and minimum detection limit of 9.4 viral genome copies. it was shown to be highly specific, as tissues from uninfected chickens and other viral genomes, such as those of marek's disease virus, fowlpox virus and infectious laryngotracheitis virus did not pr ... | 2009 | 19442846 |
| lack of evidence of avian adenovirus infection among turkey workers. | zoonotic infections constitute a major public health concern. outbreaks of the sars (severe acute respiratory syndrome) and avian influenza viruses are but recent examples. although there are many animal-specific adenoviruses and occasionally they have been noted to infect man, rarely have they been studied as potential zoonotic pathogens. in this study, the authors hypothesized that the hemorrhagic enteritis virus (hev), an avian adenovirus that causes illness among turkeys, might infect humans ... | 2009 | 19657879 |
| first step in characterization of cis-acting sequences involved in fowl adenovirus 1 (celo) packaging and its effect on the development of a helper-dependent vector strategy. | adenovirus-based vectors are widely developed for potential utilization as vectors in vaccine and gene therapy strategies. we focused on developing a helper-dependent adenoviral (hd-ad) vector for the potential use of celo, a member of the aviadenovirus genus, in avian species vaccination. our aim was to localize sequences which could play an essential role in celo genome encapsidation and, when deleted, was unable to produce viruses to develop a helper celo virus. a panel of 6 mutants with dele ... | 2009 | 18825496 |
| ceramic powder made from chicken feces: anti-viral effects against avian influenza viruses. | ceramic powder prepared by sintering of chicken feces, when mixed with avian influenza viruses or an avian adenovirus, inactivated these organisms to below detection levels. when the ceramic powder was mixed with double-distilled water, the ph of the water rose to 10 but the aqueous phase did not show any antivirus activity. after 10 washings with water or five washings with 1m tris-hcl (ph 8.0), the ceramic powder still retained antivirus activity. antivirus activity was not affected by the pre ... | 2009 | 19432001 |
| identification of group i-iii avian adenovirus by pcr coupled with direct sequencing of the hexon gene. | polymerase chain reaction (pcr) coupled with direct sequencing of the product of the hexon gene was applied to avian adenoviruses (formerly group i-iii). the expected sizes of dna fragments were successfully amplified by pcr from all of the group i-iii avian adenoviruses with our designed primers. the resulting pcr product contained diagnostically relevant hexon sequences that could be used to identify the group and type of avian adenovirus. | 2009 | 19801907 |
| genetic characterization of fowl adenoviruses isolated from chickens with hydropericardium syndrome in japan. | we genetically characterized fowl adenoviruses (serotype 4 fadv, fadv-4) isolated from chickens with hydropericardium syndrome (hps) in japan by the polymerase chain reaction (pcr) method coupled with direct sequencing. phylogenetic analysis based on the part of the hexon gene that included the l1 region revealed that all fadv-4 isolates from chickens with hps in japan were identical and were distinguished completely from the cluster including fadv strains from chickens with hps in india and pak ... | 2009 | 19959895 |
| siadenovirus infection in two psittacine bird species. | consensus polymerase chain reaction was used to identify a novel adenovirus from two psittacine birds: a plum-headed parakeet (psittacula cyanocephala) with lethargy, weight loss, and marked leukocytosis; and an umbrella cockatoo (cacatua alba) with lethargy, weight loss, and feather abnormalities. phylogenetic and comparative sequence analysis suggested that this virus is a member of the genus siadenovirus, and is here termed psittacine adenovirus 2. this extends the characterized adenoviruses ... | 2009 | 19937528 |
| immunization trials with an avian chlamydial momp gene recombinant adenovirus. | chicks were inoculated with a live vector vaccine of avian chlamydial momp gene recombinant adenovirus to evaluate efficacy, safety and viability of the vaccine. five batches of the recombinant adenovirus vaccines, which were prepared using the 22nd generation avian chlamydial momp gene recombinant adenovirus cultured in hek293 cells, were used to vaccinate 7 days-old chicks negative for chlamydial antibody. the recombinant adenovirus vaccine was shown to be both safe and effective in inducing s ... | 2010 | 21327059 |
| combating chlamydiosis. | chlamydophila psittaci is a zoonotic, intracellular bacterium, found in a wide range of birds, and is the causative agent of psittacosis, or avian chlamydiosis (ac). ac, as well as having significant zoonotic potential, causes high mortality in domestic birds, and there is no effective commercial vaccine. the group of zhou et al. has generated recombinant adenovirus vaccines, which exhibit significant potential for the prophylactic treatment of poultry. reduction of ac will affect not only the d ... | 2010 | 21327061 |
| the first complete genome sequence of a non-chicken aviadenovirus, proposed to be turkey adenovirus 1. | the complete genome sequence of an adenovirus, isolated from turkey and proposed to be turkey adenovirus type 1 (tadv-1), was determined to extend our knowledge about the genome organisation and phylogeny of aviadenoviruses. the longest adenovirus genome, consisting of 45,412 bp, with the highest g+c content (of 67.55%) known to date, was found. the central part of the tadv-1 genome has the conserved gene set and arrangement that are characteristic for every other adenovirus analysed to date. th ... | 2010 | 20709116 |
| antibody fragments, expressed by a fowl adenovirus vector, are able to neutralize infectious bursal disease virus. | single-chain variable fragments (scfv) contain the heavy and light chain variable domains of immunoglobulin, joined by a short peptide linker. previously, our laboratory has produced neutralizing scfv to epitopes of infectious bursal disease virus (ibdv). the in vitro delivery and expression of one of these scfv with and without the c(h)2-c(h)4 fc domain of chicken igy attached (scfv-fc) by a serotype 8 fowl adenovirus (fadv-8) vector was investigated in the present study. a panel of fadv-8 vect ... | 2010 | 20954010 |
| isolation and identification of a fowl adenovirus from wild black kites (milvus migrans). | a fowl adenovirus was isolated from wild black kites (milvus migrans) that died around kashipur, uttarakhand, india. this virus isolate produced cytopathic effects in chicken embryo liver (cel) cells and reacted with fowl adenovirus 4 (fadv-4) antiserum in agar gel immunodiffusion and immunofluorescence tests. the virus isolate was neutralized by fadv-4 antiserum with a neutralization titer of 800. electron microscopy of infected cel cells showed the presence of hexagonal virion particles measur ... | 2010 | 20090043 |
| characterization of fowl adenovirus serotype 4 isolated from chickens with hydropericardium syndrome based on analysis of the short fiber protein gene. | the sequences of short fiber genes of the fowl adenovirus serotype 4 (fadv-4), including isolates from chickens with hydropericardium syndrome (hps), in japan, india, and pakistan were compared. by phylogenetic analysis based on complete nucleotide sequences of this gene, fadv-4 strains from hps (hps-fadv-4) in japan, india, and pakistan fell into a different cluster from fadv-4 strains not derived from hps. hydropericardium syndrome-fadv-4 isolates were differentiated from other fadv-4 strains ... | 2010 | 20224079 |
| pyrosequencing analysis for a rapid classification of fowl adenovirus species. | a rapid fowl adenovirus (fadv) classification method based on a 30-bp sequence of the hexon loop (l1) was developed using the pyrosequencing technique. fadv identification is relevant for epidemiological studies and for the adoption of a correct strategy where vaccination is to be used for the control of the disease. fadv typing is usually performed using polymerase chain reaction coupled with either conventional dna sequencing or restriction enzyme analysis; however, both methods can be time co ... | 2010 | 20954016 |
| molecular characterization and phylogenetic analysis of an avian adeno-associated virus originating from a chicken in china. | the use of adeno-associated viruses (aavs) as vectors for gene delivery is well established; however, genomic information about avian adeno-associated virus (aaav) and its use are still limited. in this study, an aaav strain, yz-1, was isolated from healthy chickens in china, and the complete genome was sequenced. the genomic dna of yz-1 is 4,684 nucleotides long, including two orfs encoding the nonstructural proteins (rep) and the structural proteins (cap), and an inverted terminal repeat (itr) ... | 2010 | 20890715 |
| preparation and evaluation of chicken embryo-adapted fowl adenovirus serotype 4 vaccine in broiler chickens. | the current study was planned to develop an efficient vaccine against hydropericardium syndrome virus (hsv). currently, formalin-inactivated liver organ vaccines failed to protect the pakistan broiler industry from this destructive disease of economic importance. a field isolate of the pathogenic hydropericardium syndrome virus was adapted to chicken embryos after four blind passages. the chicken embryo-adapted virus was further serially passaged (12 times) to get complete attenuation. groups of ... | 2010 | 20878234 |
| a region at the left end of the fowl adenovirus 9 genome that is non-essential in vitro has consequences in vivo. | the regions at the left and right ends of fowl adenovirus (fadv) genomes are not well-characterized in comparison to those of human adenoviruses. using a series of deletion mutants, we analysed a 2.4 kb region near the left end of the fadv-9 genome (nt 400-2782) that contains packaging-signal motifs vi and vii and open reading frames (orfs) 0, 1, 1a, 1b, 1c and 2. viable viruses with specific deletions in this region had wild-type characteristics in vitro, as measured by cytopathic effect, plaqu ... | 2010 | 19759237 |
| pathogenicity and complete genome sequence of a fowl adenovirus serotype 8 isolate. | in this study we determined and analyzed the complete nucleotide sequence of the genome of a fowl adenovirus serotype 8 (fadv-8) isolate and examined its pathogenicity in chickens. the full genome of fadv-8 was 44,055 nucleotides in length with a similar organization to that of fadv-1 and fadv-9 genomes. no regions homologous to early regions e1, e3 and e4 of mastadenoviruses were recognized. along with fadv-9, fadv-8 has only one fiber gene and with regard to sequence composition and genome org ... | 2011 | 21237223 |
| Duplex PCR assay for the detection of avian adeno virus and chicken anemia virus prevalent in Pakistan. | Avian Adeno viruses and Chicken Anemia Viruses cause serious economic losses to the poultry industry of Pakistan each year. Timely and efficient diagnosis of the viruses is needed in order to practice prevention and control strategies. In the first part of this study, we investigated broilers, breeder and Layer stocks for morbidity and mortality rates due to AAV and CAV infections and any co-infections by examining signs and symptoms typical of their infestation or post mortem examination. In th ... | 2011 | 21923956 |
| application of high-resolution melting curve analysis for typing of fowl adenoviruses in field cases of inclusion body hepatitis. | fowl adenoviruses (fadvs) cause inclusion body hepatitis (ibh) in chickens. in this study, clinical cases of ibh from australian broiler flocks were screened for the presence and genotype of fadvs. | 2011 | 21495991 |
| detection of chicken proventricular necrosis virus (r11/3 virus) in experimental and naturally occurring cases of transmissible viral proventriculitis with the use of a reverse transcriptase-pcr procedure. | a reverse-transcriptase-polymerase-chain-reaction (rt-pcr) procedure was evaluated for detection of chicken proventricular necrosis virus (cpnv) in transmissible viral proventriculitis (tvp) -affected chickens. the rt-pcr procedure was compared with indirect immunofluorescence (ifa) and virus isolation for detection of cpnv in experimentally infected chickens. microscopic lesions characteristic of tvp were detected on days 5-35 postexposure (pe) in cpnv-infected chickens; cpnv was detected by rt ... | 2011 | 21500639 |
| partial sequence of the dna-dependent dna polymerase gene of fowl adenoviruses: a reference panel for a general diagnostic pcr in poultry. | adenoviruses are frequent infectious agents in different poultry species. the traditional, serological typing of new isolates by virus neutralisation tests is now in transition to be replaced by pcr and sequencing. the first pcrs, recommended for the detection of adenoviruses, had been designed to target the gene of the major capsid protein, the hexon. in birds, members of three different genera of the family adenoviridae may occur. accordingly, three specific hexon pcrs had to be elaborated for ... | 2011 | 21665581 |
| Full genome analysis of a novel adenovirus from the South Polar skua (Catharacta maccormicki) in Antarctica. | Adenoviruses have been identified in humans and a wide range of vertebrate animals, but not previously from the polar region. Here, we report the entire 26,340-bp genome of a novel adenovirus, detected by PCR, in tissues of six of nine South Polar skuas (Catharacta maccormicki), collected in Lake King Sejong, King George Island, Antarctica, from 2007 to 2009. The DNA polymerase, penton base, hexon and fiber genes of the South Polar skua adenovirus (SPSAdV) exhibited 68.3%, 75.4%, 74.9% and 48.0% ... | 2012 | 22078165 |