Publications

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[attempts at isolating celo virus in bulgaria]. 19744377475
pathogenesis and pathology of infection with two strains of avian adenovirus. 19744377151
pathogenicity of avian adenoviruses for day-old chicks. 19744377152
[study of the pathogenic qualities of 4 celo-virus strains isolated in bulgaria].four celo virus strains, isolated in this country, were used to infect 3-day-old chicks via the trachea, muscularly, orally, subcutaneously, and cerebrally. the chicks were killed on the 2nd, 4th, 6th, 9th and 16th day following inoculation. material was taken from each chick for morphologic, virologic, and serologic examination. the clinical course of the disease was also followed up. prior to killing some birds showed infirmity, difficult breathing, slightly expressed diarrhia, going off food, ...1975179190
[the significance of celo virus as a hatching egg contaminant with special reference to its oncogenic properties].a group of young hamsters was injected subcutaneously in the neck region with allantoic fluid containing celo virus. a second group received transplants of cells from tumours induced by celo virus. tumours developed in both groups. the latent period was shorter after transplantation than after virus inoculation. the tumours grew progressively, histologically resembling fibrosarcoma. it is recommended that hatching eggs infected with celo virus should not be used in vaccine production.1975180924
hemagglutinating properties of celo, an oncogenic avian adenovirus.chicken-embryo-lethal-orphan (celo) virus, phelps strain, agglutinated erythrocytes at 37 c. the hemagglutinating activity, which is a function of complete and incomplete virus particles, was sensitive to heat but not to ph. the soluble components of the virus were similar in sedimentation characteristics to those obtained from human adenovirus type 1. the effects of chemical and physical agents on celo hemagglutinin, celo infectivity, and red-cell receptors suggested that the last were protein ...1975173279
a study of the sequences of chicken embryo lethal orphan (celo) virus dna present in a transformed hamster cell line with use of specific fragments of the virus genome. 1975173080
cell-to-cell attachments and associations in tumors induced by celo virus or virus-transformed cells in hamsters.cell-to-cell attachments or associations in expermentally induced sarcoma of chicken embryo lethal orphan virus (avian adenovirus) origin were studied in hamsters by electron microscopic examination. in many instances, the neoplastic cells seemed to be held together either by desmosome-like structures or an "interlocking" of their apposing plasma membranes. less frequently, the cells were attached by button-like projections between the cell surfaces. only rarely, interdigitation occurred between ...1975171984
transplatation resistance to adenovirus-induced hepatocellular carcinomas.two transplantable hepatic carcinomas of the hamster contained celo virus-induced tsta, confirming the etiological role of this avian adenovirus in the induction of hepatic tumors. transplantation resistnace could not be induced with oncogenic adenoviruses of human, simian, or canine origin.1975170623
a circular dna-protein complex adenoviruses and its possible role in dna replication. 1975169089
covalent integration of viral dna into cell dna in hamster cells transformed by an avian adenovirus. 1975165621
the specificity of cellular immune reactions to three dna virus induced tumours, as measured by the macrophage migration inhibition test. 197548461
[physico-chemical properties of celo virus strains isolated in bulgaria].studied were some physico-chemical properties of four celo virus strains isolated in bulgaria. their behaviour to ether, chloroform and 25 per cent trypsin, and their state at 56 degrees c and at ph 3, 0 were determined through their infectious titer as obtained on 9--10-day old chicken embryos. it was found that the size of the investigated viruses is within the range of 50 to 100 mmu, they belong to the dna-possessing group, are resistant at 56 degrees c and at ph equals 3,0. they are not sens ...1975236618
adenoviridae.the elevation of the genus adenovirus to a family named adenoviridae is described. this family at the present stage contains two genera, mammalian adenoviruses--mastadenovirus--and avian adenoviruses--aviadenovirus. the two genera are separated primarily on the basis of difference in immunological properties of the virions.19761002415
avian cell cultures grown in an open system (dishes) without co2 incubator, and their use for virus cultivation.an organic buffer, n-2-hydroxyethylpiperazine-n'-2-ethanesulfonic acid (hepes), in a culture medium made it possible to culture chick embryo fibroblast (cef), duck embryo fibroblast (def), and chick kidney (ck) cells in an open system (dishes) under incubation in a bacteriological incubator, requiring no co2 incubation. cef, def, and ck cells cultured with this system were suitable for cultivation of avain viruses such as newcastle disease virus, celo virus, marek's disease virus, and turkey her ...1976186014
the oncogenic potential of some avian adenoviruses causing diseases in chickens.the oncogenic potentials of three different strains of avian adenoviruses (the tipton strain of the inclusion body hepatitis virus, the dpi-2, and the indiana c viruses) were investigated in newborn hamsters. animal inoculations were via two routes, subcutaneous and intracerebral. all three viruses proved nononcogenic for the hamsters observed over a period of 225 days. however, lesions of hepatitis similar to those of inclusion body hepatitis of chickens were seen in three hamsters inoculated w ...1976176986
infection of the chicken with an avian adenovirus and myocoplasma gallisepticum. 1976176183
tenosynovitis in chickens.an adenovirus was isolated from the tendon fluid of broilers and meat breeders with clinical tenosynovitis. the viral infection was in many cases accompanied by a staphylococcal infection. the significance of the adenovirus is not yet known, although initial transmission studies indicate that it may play a role in the tendon thickening process.1976189747
an adenovirus from a turkey pathogenic to both chicks and turkey poults.an adenovirus, designated t-75 isolant, was isolated from the cloacal swab of a clinically normal turkey, 13 weeks old. the t-75 isolant was identified as an adenovirus on the basis of physicochemical properties, cytopathology, and agar-gel precipitin test. producing two-way cross-neutralization reactions with celo virus, the isolant was classified as an avian adenovirus of serotype 1. the t-75 isolant was pathogenic to both chicks and turkey poults, causing hepatitis, respiratory disease, atrop ...1976186010
some properties of avian adenoviruses isolated from chickens with inclusion body hepatitis in japan.outbreaks of inclusion body hepatitis were observed in broiler chickens on a poultry farm during 3 years. avian adenovirus-like agents were isolated during these years from livers of diseased chickens. round-cell-type cytopathogenic effect and intranuclear inclusion bodies were produced in chicken kidney cell cultures inoculated with these agents. properties of the agents were as follows: resistant to ether, chloroform, socium deoxycholate, trypsin, heating at 50 c, and ph 3.0; sensitive to 5-io ...1976186009
a technique for isolating turkey respiratory adenoviruses.several turkey respiratory adenoviruses were isolated in turkey embryonic liver cells from nasal turbinate filtrate collected from clinically ill turkey poults during postmortem examination. suspect adenovirus inoculum had to remain in cell culture for 5 to 7 days, and 9 blind passages were required before a sample was declared negative. once isolated, the turkey adenoviruses adapted rapidly to turkey kidney cells.1976186007
avian adenoviruses isolated from poultry in queensland.adenoviruses isolated from queensland poultry flocks with respiratory disease and/or egg production problems have been serotyped by comparison with japanese and irish types. two serological types were recognized among 14 isolates examined; 4 were type 112 (celo type) and the remaining 10 type 506. isolates b1363 and b4015 were further characterized by immunodiffusion. both isolates were shown to contain the avian adenovirus group antigen and b1363 also shared a second (type) antigen with celo (p ...1976190991
effect of formaldehyde on disinfection of filtered air under positive pressure (fapp) type house.effect of formaldehyde liberated from formalin on disinfection of the surface of a filtered air under positive pressure (fapp) house and the surface of equipment placed in the house was studied. escherichia coli, staphylococcus aureus and avian adenovirus were used in this study as a indicator to evaluate degree of disinfection. satisfactory degree of disinfection of the house and equipment was obtained when formaldehyde was liberated from 40 ml. of formalin per 1 m.3 capacity. bacteria and viru ...1976190599
a study of two avian adenovirus serotypes alone or in combination with avian infectious bronchitis in day-old chicks. 1977190275
[hemorrhagic enteritis of turkeys-- 1]. 1977189984
adenovirus of ring-necked pheasants: purification and partial characterization of marble spleen disease virus.a method for purification of marble spleen disease virus (msdv) from the spleens of infected turkeys and pheasants is described. it combines chloroform or fluorocarbon extraction with subsequent purification by centrifugation on a cushion of cesium chloride (cscl). further purification of msdv was accomplished with a cscl equilibrium density gradient. a viral buoyant density of approximately 1.32 to 1.33 g/cm3 was determined. negative-stain electron microscopy revealed that virus isopycnically b ...1977193796
tenosynovitis in chickens. 1977194565
chick embryo lethal orphan (celo) virus-induced early and late polypeptides. 1977193270
serotyping avian adenoviruses by a microneutralization procedure.a microneutralization procedure, using chicken kidney cell monolayers as an indicator system, was developed and applied to the serotyping of isolates characterized as avian adenoviruses. the method was determined to be reproducible, since coefficients of variation were low for 12 replicate titrations of homologous reagents of 9 prototype avian adenoviruses. prototype reagents were specific according to results of reciprocal end point-neutralization tests and comparison of antigenic relatedness, ...1977192109
involvement of a type-8 avian adenovirus in the etiology of inclusion body hepatitis.type-8 avian adenoviruses were isolated from chickens in a commerical flock suffering an outbreak of inclusion body hepatitis. serum-neutralizing titer to this type, but not to 7 other types of avian adenovirus, was more than 4 times as high in convalescing chickens as in chickens from the flock bled 2 weeks previously, during the disease outbreak. a disease similar to that in the commercial flock and to inclusion body hepatitis as described in the literature was produced by intra-abdominal inoc ...1977190994
demonstration of an avian adenovirus as the causative agent of marble spleen disease.a purification procedure, using chloroform or fluorocarbon extraction and centrifugation on a cushion of cesium chloride (cscl), was designed to isolate the causative virus of marble spleen disease. virus was purified, inoculated into turkeys, and subsequently reisolated by purification from the spleen of inoculated turkeys, thus fulfilling koch's postulates. splenic antigen was detected by the agar gel precipitin test, and viral inclusions with viral particles were observed by light and electro ...1977189649
virus-neutralizing antibody titers against 8 avian adenovirus serotypes in breeder hens in georgia by a microneutralization procedure.serums from 16 chicken-breeder flocks in georgia were tested for virus-neutralizing antibody to 8 serotypes of avian adenovirus. titers to all 8 serotypes were demonstrated in 8 of the flocks, titers to 7 in 6, and titers to fewer than 7 in the other 2 flocks. although titers were high overall to some serotypes (types 2 and 8) and low to others (types 1, 4, and 5), with statistically significant differences between many titers, the data were difficult to interpret because of possible heterotypic ...1977194569
pathology of adenoviral infection in turkeys (meleagris gallopavo) with respiratory disease and colisepticemia.nuclear inclusion bodies typical of the adenovirus group were widespread in in the spleen and other tissues of 8-week-old turkeys with severe respiratory disease and concomitant evidence of colisepticemia. adenoviral virions were seen in affected nuclei of splenic tissue and in negatively stained preparations of ground spleen. in splenic tissue, inclusions were most prominent in reticular cells and macrophages in the periarterial lymphoid sheaths, the red pulp and the marginal zones of the peria ...1977201075
physicochemical characterization and pathogenicity studies of two turkey adenovirus isolants.two turkey adenoviruses were isolated from poults with respiratory disease, and their physicochemical properties were studied. the virus particles were unenveloped. contained dna genome, replicated within the nuclei of infected cells, and were icosahedral in shape. pathogenicity studies in poults indicated that north carolina kennedy isolant was a pathogen of relatively low virulence whereas the north carolina jackson isolant was nonpathogenic in commercial turkey poults.1977204278
further studies on the classification of fowl adenoviruses.in addition to the 8 fowl adenovirus serotypes described previously, 3 new serotypes were recognized. two, a-2 and c2-b, were isolated in the u.s.a., and one, 380, in northern ireland. in addition another isolate, x-11, has a broader antigenicity than yr-36 and therefore should be used instead of yr-36 as prototype strain. it is concluded that there are at least 11 distinct fowl adenovirus serotypes on the basis of the serum-neutralization test.1977204279
antigenic analysis of several turkey respiratory adenoviruses by reciprocal-neutralization kinetics.five turkey adenoviruses were classified into four serologic groups by reciprocal-neutralization kinetics. two viruses were found to be serologically homologous, while three others were found to be heterologous and placed in separate serologic groups.1977204280
broad antigenicity exhibited by some isolates of avian adenovirus.ten of 16 isolates of avian adenovirus could be readily classified as members of serotypes 2, 5, or 7. however, 6 exhibited broad neutralization reactions which created typing problems. detailed studies of these interactions revealed that our prototype for serotype 5 (i.e., t-8; subsequently found to be an "intermediate" strain) contributed to this broad cross-reactivity because it shared antigens with members of serotypes 5 and 7. as a result of this study, tr-59 or 58-1 is recommended as the s ...1977196532
serological response of chickens exposed to a type 1 avian adenovirus alone or in combination with the adeno-associated virus.the avian adenoviruses (av) are common infectious agents of poultry and other avian species throughout the world (1,4,8). limited observations suggest that the adeno-associated virus (a-av) coinfects many of the chickens that carry av (8). the presence and persistence of these infections in a flock is often determined by serological methods. in the current study, the immune response of chickens to type 1 av alone and to a dual exposure, av plus a-av, was followed over a 12-week period with a var ...1977199153
immunization of chickens against adenovirus infection.chickens were vaccinated with monovalent and polyvalent adenovirus vaccines using three different serotypes, types 1, 2, and 3. no cross-protection was elicited by the heterologous serotypes but satisfactory protection from lesions and the shedding of challenge virus was induced by the homologous serotypes in the monovalent and polyvalent vaccines. the virus neutralizing (vn) titers were occasionally lower where the polyvalent vaccines were used when compared to those from chickens given the mon ...1977203922
effect of maternal antibody on experimental infections of chickens with a type-8 avian adenovirus.mortality was 60% when chickens without detectable maternal antibody to avian adenoviruses were inoculated intra-abdominally with 10(6) plaque-forming units of amg 5(2a), a type-8 avian adenovirus. other results were macroscopic and microscopic lesions in a wide range of organs, statistically significant depression of body weights, amg 5(2a) virus in the liver and feces, and high virus-neutralizing antibody titers to amg 5(2a). the disease produced was similar to that described in a previous rep ...1977190997
procedure for the isolation of avian adeno and avian adeno-associated viruses. 1977194575
[isolation and detection of avian adenoviruses on cell cultures].it is important, from the hygienic and epizootological view-point, to detect the presence of various microbiological agents in birds. the authors present the results of the virological examination of poultry, avian adenoviruses being the main object of investigation. ninety-eight virus strains were isolated from chickens of different age categories and were included in the group of avian adenoviruses according to the form of cytopathic changes on the cell cultures of hen kidneys, according to th ...1978208217
comparative study of experimental inclusion body hepatitis of chickens caused by two serotypes of avian adenovirus.twenty 1-day-old specific-pathogen-free chickens each were given an intraabdominal inoculation of either a type-8 avian adenovirus, [amg 5 (2a], or a type-5 avian adenovirus, inclusion body hepatitis virus (ibhv). the diseases produced were similar. high (60-100%) mortality and statistically significant depression of body weights occurred in both infections. there were necrotizing hepatitis and pancreatitis, lymphoid depletion in the spleen, bursa of fabricius and thymus, hydropericardium, nephr ...1978208221
an adenovirus survey of poultry flocks during the growing and laying periods.chicken adenoviruses were isolated from asymptomatic chickens on each of 7 farms tested; a majority of them induced cytopathology by the second serial passage. although adenoviruses were isolated from chickens ranging in age from 8 to 34 weeks, the highest isolation rate was from those 8 to 14 weeks. eight serotypes (1, 2, 4, 5, 6, 7, 8, and 9) were identified in a relatively small geographic area; serotypes 1, 4, 7, and 9 were isolated most frequently. multiple serotypes were found on 6 of 7 fa ...1978206248
cell cycle-dependent multiplication of avian adenoviruses in chicken embryo fibroblasts.propagation of celo virus employing confluent monolayers of chicken enbryo fibroblasts (cef) yielded virus titers one to two logs lower than those from confluent chicken kidney (ck) cells. an enhancement of virus production in cef as measured by plaque formation was obtainedby infectng cultures in the growing non confluent state. measurements of 3h-thymidine incorporation revealed a positive correlation between the dna synthesis of cef cultures at the time of inoculation and the amount of progen ...1978209769
avian adenoviruses--distribution of infection in commercial flocks of fowls in argentina.group-specific avian adenovirus antibodies were surveyed in broiler, layer, and breeder chickens in three poultry-producing areas of argentina: north, middle, and south. the method used was the agar double-immunodiffusion test. of the flocks examined, 89% had antibodies to avian adenovirus, with the percentages higher in layers and breeders than in broiler chickens. a close relation was found between poorer hygienic condition and high adenovirus antibodies values.1978219837
[structure of inclusion bodies and electron microscopic virus detection in naturally occurring inclusion body hepatitis in chickens].electron microscopy was used to examine the liver of chickens with spontaneous inclusion body hepatitis. eosinophilic inclusion bodies only were established from two flocks, mainly amphophilic from one flock, and primarily basophilic from another two flocks. eosinophilic inclusion bodies were predominant in broiler chickens with dystrophic fatty degeneration of the liver, while basophile inclusion bodies were recorded primarily from parental or laying-hen chickens with reduced metabolic stress o ...1978219796
hemagglutination-inhibition antibodies against an adenovirus (virus-127) in white pekin ducks in the united states. 1978219838
egg drop syndrome 76. 1978207005
virus-specific markers and virus-like particles in cell lines of tumors produced by celo virus in syrian golden hamsters.cell lines were established from 2 primary hepatocellular carcinomas (hc's) and 3 sarcomas produced in syrian golden hamsters inoculated as newborns with chicken embryo lethal orphan (celo) virus. cell lines from 2 sarcomas (cot, cmt) and 1 hc (cehep) produced celo virus-specific t-antigen. the antigen was not detected in cells of the third sarcoma line (rct) until they had undergone more than 34 passages in vitro. although 5-10% of cells in the second hc line (cilt/2) contained t-antigen during ...1978209200
[attempts to demonstrate and identify avian adenoviruses by an immunofluorescence method].a specific conjugate of a hyperimmune serum, obtained from rabbits with the use of the reference phelps-celo strain, was employed in a direct immunofluorescence method to investigate more than 320 lamelles with cell cultures infected with 4 field celo virus strains and the reference phelps-celo strain. it is stated this method is specific and prompt for the early diagnosis and identification of adenoviruses (celo viruses).1978210562
avian adenoviruses: effect on egg production, shell quality, and feed consumption.four avian adenovirus isolants (indiana c, dpi-2, ibh-2, and b-3)were examined for pathogenicity in laying birds. none affected shell quality, and only one (ibh-2) had even a minimal effect on egg production (faster decline). of three isolants tested (indiana c, dpi-2, and ibh-2), only ibh-2 caused feed intake to be reduced. no other clinical signs were observed during these experiments. egg transmission, virus excretion, and antibody production were found to mimic patterns reported for serotype ...1978212004
egg-drop syndrome 76 in danish poultry.after a more general mention of egg drop syndrome 76 (eds 76) an account is given of 3 cases of eds 76 in broiler parent flocks. in all cases the animals involved originated from ireland and were imported as day-olds, either directly from ireland or via sweden. serological examinations indicate that the infectious agent has not previously been present in danish hen flocks, but that it is presumably stationary in danish flocks of ducks. in 2 of the 3 cases of eds 76 animals from 2 different forei ...1978214753
subclinical pneumonia associated with an experimental adenovirus infection in the domestic fowl and the effect of concurrent infectious laryngotracheitis virus.a celo-type adenovirus (av) isolated from fowls with respiratory disease was inoculated experimentally into the tracheas of young birds. no symptoms referable to respiratory infection were evident. post mortem examination between days 2 and 5 after inoculation revealed pneumonia involving up to 30 per cent of the surface of the lungs. histologically, a focal to diffuse interstitial lymphocytic infiltration and bronchiolar degeneration were present. concurrent infections with a mild strain of inf ...1978203988
experimental induction of hemorrhagic-aplastic anemia in chickens. i. etiology.exposure to infectious bursal disease virus (ibdv) at 1 day old followed by inclusion body hepatitis virus (ibhv) inoculation at 36 days produced typical lesions of hemorrhagic-aplastic anemia syndrome (has). the lesions included severe anemia, widespread hemorrhages, and dermatitis. has could not be induced in the first 4 weeks of life in chickens inoculated at one day old with ibhv alone or in combination with ibdv. it was concluded that the immunosuppressive effects of ibdv failed to alter th ...1978219831
serologic and pathogenicity studies of avian adenovirus isolated from chickens with inclusion body hepatitis.a virus initially thought to be inclusion body hepatitis virus (ibhv), tipton strain, was classified as an avian adenovirus (aav) and shown to be antigenically related to 2 serotypes of aav, 764 and yr36. the virus was antigenically unrelated to aav serotype tr-22, which included ibhv, tipton strain. inoculating specific-pathogen-free chickens with the virus produced hepatitis with basophilic and eosinophilic staining intranuclear inclusion bodies.1978206251
failure to detect common antigenic hemagglutinating components in avian adenoviruses. 1978206254
chicken embyro lethal orphan (celo) virus dna present in hamster cell lines derived from celo-induced hepatomas.the kinetics of renaturation of the isolated dna fragments of chicken embryo lethal orphan (celo) virus generated by the restriction enzyme ecor1 was measured in the presence of dna extracted from two lines of celo virus-induced hamster hepatoma cells and from control hamster cells. one hepatoma cell line (cilt-2), which did not produce the celo virus tumor (t) antigen, lacked sequences from about one-half the virus genome while the other hepatoma cell line (cehep), which did produce the celo vi ...1978202577
some adenovirus dna is associated with the dna of permissive cells during productive or restricted growth.we have investigated the association of viral dna with cell dna in chicken embryo kidney (cek) cells productively infected with chicken embryo lethal orphan (celo) virus and in human (hek) cells infected with mutants ts36 and ts125 of human adenovirus type 5 under permissive and restrictive conditions. cell and viral dna molecules were separated after celo virus infection of cek cells by alkaline sucrose gradient centrifugation, network formation, and cscl density gradient centrifugation, method ...1978202728
isolation of a virus indistinguishable from adenovirus 127 from chickens in the united states. 1978221936
effect of avian adeno-associated virus on pathogenicity of tipton virus in chicks.the avian adeno-associated virus (a-av) reduced the pathogenicity of an adenovirus infection in vivo. groups of chicks were infected with tipton virus alone or in combination with high or low doses of a-av. in both trials, the associated virus delayed and reduced chick mortality. this effect was dose-dependent and significant at the higher dose level.1978209781
avian adenoviruses and tenosynovitis. 1978207011
isolation of a serotype 8 avian adenovirus associated with inclusion body hepatitis. 1979231963
egg drop syndrome, 1976 (eds'76).a description is given of the new clinical condition in fowl now known as eds 76. this is characterised by the production of thin shelled, soft shelled and shell less eggs by apparently healthy birds. the effects on egg production are seen around 30 weeks of age and are seen either as an apparent fall in production or as a failure to obtain predicted production targets. haemagglutinating viruses have been isolated from these flocks and evidence from experimental and field investigations suggest ...1979227136
[dna fragmentation of chicken adenovirus celo by specific endonucleases r. hpai, r. ecori, r. hindiii].the effect of specific endonucleases on dna chiken adenovirus celo was studed. it was shown that endonucleases r. hpai, r. ecori and r. hindiii cleaved viral dna into 5,7 and 9 specific fragments, respectively. the sequence of fragments (physical map) was determined and found to be: d-a-e-c-b for enzyme r. hpai; b-(eg)-c-a-d-f for enzyme r. ecori; h-f-a-c-g-b-d-e-i for enzyme r. hindiii.1979228178
a serological survey for the presence of antibodies to 'egg-drop-syndrome-1976' adenovirus. 1979229445
adenovirus-associated virus-precipitating antibody as an early indicator of an adenovirus infection. 1979223478
inhibition and enhancement of avian adenovirus plaque production by heavy and light avian adenovirus-associated viral particles.the effect of heavy and light avian adenovirus-associated viral (a-av) particles on the replication of several adenovirus serotypes was studied in chicken embryo kidney cells. there was a significant decrease (p less than 0.05) in the number and size of adenovirus-induced plaques at a-av multiplicities of infection greater than 40. enhancement of plaque production was observed when a-av multiplicities of infection were 1 to 40. there was a significant increase in the number and size of infective ...1979229748
unusual features of the oncogenicity of chicken embryo lethal orphan (celo) virus in hamsters. 1979230532
susceptibility of ducks to the ote strain (celo) of avian adenovirus. 1979225595
egg transmission of avian adenovirus-associated virus and celo virus during a naturally occurring infection.both avian adenovirus-associated virus (a-av) and celo virus were isolated from the embryonating eggs of 25-week-old black sex-linked hens during a naturally occurring infection. in the first 7 days of egg collection, a-av was isolated from 10 of 43 (23.2%) embryonating eggs, and celo virus was isolated from 8 of 43 (18.6%) embryonating eggs. both viruses were isolated from six eggs. in the next 16 days of egg collection, a-av and celo virus were coisolated from 1 of 127 (0.8%) eggs; all other s ...1979230761
transmission of acute respiratory disease (rhinotracheitis) of turkeys.clinical signs of acute respiratory disease of turkeys were transmitted to susceptible day-old poults by direct contact, litter contact, and drinking water. attempts failed to transmit the disease by air (cage-to-cage) or by oral and nasal inoculation with feces, nasal exudates, or nasal turbinate extracts. the disease was not transmitted by inoculation with white blood cells. chicks were not affected by the disease, but young quail developed signs of respiratory disease when exposed to contamin ...1979226051
[distribution of adenovirus in the organ system of chickens (preliminary report)]. 1979226060
experimental infection of broiler and leghorn chickens with virulent and avirulent isolates of hemorrhagic enteritis virus.the effects of virulent and avirulent isolates of hemorrhagic enteritis virus were studied in leghorn and broiler chickens. the viruses caused subclinical infections in both strains of birds. the avirulent isolate produced enlarged spleens, inclusion bodies, and antibody in all inoculated leghorns. in broilers the avirulent virus produced infection in only half of the inoculated birds. inclusion bodies were widely dispersed in the spleens of both types of birds. the virulent virus produced infec ...1979230804
the molecular weight of the egg drop syndrome (eds) avian adenovirus (strain b8/78) dna estimated by digestion with restriction endonuclease enzyme r x ecori. brief report.the restriction endonuclease r x ecori cleaved at two sites of the eds adenovirus (strain b8/78) dna generating 3 fragments with molecular weights of 13.5 x 10(6), 5.0 x 10(6) and 4.4 x 10(6) daltons, respectively. the estimated molecular weight of the whole undigested dna is about 22.9 x 10(6) daltons.19806252866
enhancement and inhibition of celo virus pathogenicity in quail by avian adenovirus-associated virus.dual infection of 12 day-old quail (colinus virginianus) with 10(6) plaque forming units of celo virus and low doses of avian adeno-associated virus (a-av), resulted in significant enhancement of celo virus-induced mortality, whereas dual infections with high doses of a-av resulted in a delay in mortality. a-av induced enhancement and inhibition of celo virus pathogenicity could be blocked by the addition of a-av antiserum prior to infection.19806253685
populations of infectious virus produced during avian adenovirus-associated virus infection.multiple rounds of infection in vitro or in vivo with avian adenovirus-associated virus (ava-av) and avian adenovirus (avav) result in production of both heavy (h) and light (l) infectious forms. in this study, the infectious ava-av progeny produced at different hours during recombined dual infection of cells with either h or l ava-av and avav was determined by equilibrium cscl centrifugation and infectivity assay. in both types of infection, h virions were found early, both intra- and extracell ...19806250991
adult coturnix quail bronchitis.quail bronchitis was confirmed by recovery of fowl adenovirus serotype 1 from 2 flocks of adult birds (coturnix coturnix japonica) that were reared commercially for their eggs in the republic of singapore. both flocks were reported to have a fall in egg numbers of 10-15%. the main clinical signs were respiratory distress and soft-shelled and white eggs lacking shell pigmentation.19806254496
serologic detection of adenovirus infections in specific-pathogen-free chickens.a specific-pathogen-free flock of white leghorns, which were housed conventionally and were previously serologically negative for all common poultry pathogens including avian adenoviruses, incurred an outbreak of adenovirus that was detected at about 39 weeks of age. the infection was detected serologically through the agar-gel precipitin test (agpt) and also by a microneutralization test (mnt) adapted for 11 serotypes of avian adenovirus. the mnt detected specific antibodies to serotype-3 avian ...19806254497
[significance of avian adeno-associated virus (aaav) in serological studies of adenovirus infections in chickens]. 19806258360
[antibody formation after the experimental infection of dogs with various avian adenovirus strains]. 19806260078
isolation of infectious bronchitis, infectious bursal disease and avian adenoviruses from chickens with nephritis-nephrosis syndrome. 19806255706
incidence and distribution of "avian adenovirus group ii splenomegaly of chickens".forty-six percent of 3,585 serum samples from 174 broiler-breeder chicken flocks in 9 states were positive for the precipitating antibody of hemorrhagic enteritis (he) of turkeys/marble spleen disease (msd) of pheasants. seroconversion was detected only in flocks 19 weeks of age and older. the percent of individual serum samples positive within a flock increased with the age of the flock.19806255924
egg transmission of egg drop syndrome 1976 virus in fowl.egg transmission of egg drop syndrome 1976 virus (bc14 virus) in fowl was demonstrated in the second and third week after experimental infection. eggs of bc14 virus infected hens were incubated weekly after disinfection with formaline gas. after 18 days of incubation, eggs with live embryos were homogenized. this egg material was fed to adult hens, housed in isolators. seroconversion in these birds demonstrated egg transmission. it is suggested that egg transmission occurs as a result of viremia ...19806252649
egg production in relation to the results of a long term serological survey of 73 flocks of fowl.seventy-three flocks of fowl were tested at regular intervals for the presence of precipitins to fowl adenovirus (av) and infectious bronchitis virus (ibv), haemaggluinating inhibiting antibodies to bc in 14 virus, and of agglutinins to mycoplasma gallisepticum (m.g.) and mycoplasma synoviae (m.s.). in all the eight flocks affected with egg drop syndrome (eds '76), egg production problems were associated with increasing numbers of bc14 virus reactors and av reactors. in flocks showing production ...19806243424
[dynamics of maternal and post-infection adenovirus antibodies in fowl].the epizootological study of the adenovirus infections of poultry, conducted with the use of the serum virus-neutralizing test, gives a picture of the distribution of various serotypes of avian adenoviruses in the poultry population, or in the largest poultry stocks in czechoslovakia. there is a description of the occurrence of type-specific antibodies in serums of different age categories of poultry acting against seven available virus serotypes. an ascending trend in positive findings of adeno ...19806250273
[detection of neutralizing antibodies against avian adenoviruses by a mircotiter cell culture system]. 19806250531
neutralizing antibodies to celo and avian adenovirus-associated viruses in the albumen of chicken eggs.neutralizing antibodies to celo virus and to avian adenovirus-associated virus (a-av) were detected in the albumen of eggs from four hens inoculated with these viruses. the antibody concentrations of serum, yolk, and albumen were determined before inoculation and at various times postinoculation (pi) by enzyme-linked immunosorbent assay (elisa) and virus-neutralization (vn) tests. the antibody concentration in albumen was 0.3% to 1.0% of that detected in serum and yolk. uninoculated hens showed ...19806268041
serological identification of avian adenoviruses isolated from cases of inclusion body hepatitis in victoria, australia.sixteen avian adenoviruses isolated from 12 cases of inclusion body hepatitis (ibh), 3 cases of respiratory disease, and a case of ruptured tendons were compared using antisera raised against 9 fowl adenovirus prototype strains. eleven isolates from livers of birds with ibh were classified into 4 different serological groups: 1) yr36 (type 7)-related; 2) hvi (type 8)-related; 3) variants--type 6-,7-, and s-related; and 4) type 50--not closely related to any of the prototype antisera tested. thes ...19806268046
the oncogenicity of avian adenoviruses. i. an unusually large number of viral dna molecules in some tumors, and virus-specific t-antigenic proteins. 19806254256
the oncogenicity of avian adenoviruses. ii. the arrangement of the viral dna in tumors and transformed cells. 19806254257
an enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.an enzyme-linked immunosorbent assay system (elisa) was adapted for the detection of antibodies to avian adenovirus (av) and avian adenovirus-associated virus (a-av). both before and after exposure, sera from chickens undergoing natural and experimental infections were assayed by elisa, virus neutralization (vn), and immunodiffusion (id) for antibody to both celo virus and a-av. the elisa system was found to be comparable to vn for determining antibody concentrations to celo virus and a-av. in m ...19806254491
avian haemagglutinating adenovirus antibodies in australian poultry. 19816282245
short communications. 19816270874
growth of adeno-associated virus in embryonated eggs. 19816262350
[study of viral contamination of japanese quail and cell cultures from their embryos].agents identified as adenovirus celo were isolated from organ suspensions of 1500 japanese quails (jq) in 4 experiments. no contaminating viruses were found in examinations of cell cultures prepared from the kidneys of 80 jq. negative results were obtained in examinations of 163 antigens from jq organs in the cofal test. agents identified as mycoplasma were isolated in 16 cases from 736 specimens of the virus-containing fluid used for manufacture of measles virus. according to the results of the ...19816266156
hemorrhagic enteritis: virus distribution and sequential development of antibody in turkeys.turkeys poults were inoculated intraperitoneally with hemorrhagic enteritis virus (hev) at 4-1/2 weeks of age. antibody response and sequential development of viral antigen in various tissues were monitored. an enzyme-linked immunosorbent assay (elisa) was developed to study antibody production, and immunoperoxidase staining was used to determined sites of localization of the viral antigens in tissues. results of elisa and immunodiffusion tests were compared. elisa detected antibody from day 3 p ...19816266390
turkey respiratory tract adenoviruses: oncogenic potential in neonatal hamsters (mesocricetus auratus).the tumorigenic properties of 3 turkey adenoviruses (cua, nc-k, and mst) isolated from turkeys with respiratory tract disease and injected into neonatal hamsters (mesocricetus auratus) have been determined. one of 30 adenovirus isolates (cua) induced tumors at the site of inoculation (subcutaneously or intracranially) in neonatal hamsters. the tumors were identified as fibrosarcomas and undifferentiated sarcomas. the tumors were found to be free of infective virus, but hamsters which had tumors ...19816267964
turkey respiratory tract adenoviruses: purification and some biochemical properties. 19816267965
turkey respiratory tract adenoviruses: in vitro infectivity of purified dna. 19816267966
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