Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cre recombinase-mediated restoration of nigrostriatal dopamine in dopamine-deficient mice reverses hypophagia and bradykinesia. | a line of dopamine-deficient (dd) mice was generated to allow selective restoration of normal dopamine signaling to specific brain regions. these dd floxed stop (ddfs) mice have a nonfunctional tyrosine hydroxylase (th) gene because of insertion of a neor gene flanked by lox p sites targeted to the first intron of the th gene. ddfs mice have trace brain dopamine content, severe hypoactivity, and aphagia, and they die without intervention. however, they can be maintained by daily treatment with l ... | 2006 | 16723393 |
| [construction and experimental immunity of recombinant replication-competent canine adenovirus type 2 expressing hemagglutinin gene of h5n1 subtype tiger influenza virus]. | h5n1 highly pathogenic avian influenza virus was highly pathogenic and sometimes even fatal for tigers and cats. to develop a new type of vaccine for felidae influenza prevention, recombinant replication-competent canine adenovirus type 2 expressing hemagglutinin gene of h5n1 subtype tiger influenza virus was constructed. a/tiger/harbin/01/2003 (hsn1) ha gene was cloned into pvax1. the ha expression cassette which included cmv and ha and polya was ligated into the e3 deletion region of pvaxdelta ... | 2006 | 16736595 |
| retracted: development of recombinant canine adenovirus type-2 expressing tsol18 of porcine cysticercus. | this article has been retracted consistent with elsevier policy on article withdrawal. please see . the publisher apologizes for any inconvenience this may cause. | 2006 | 17095028 |
| comparative trial of the canine parvovirus, canine distemper virus and canine adenovirus type 2 fractions of two commercially available modified live vaccines. | the results of vaccinating two groups of puppies with commercial vaccines, both of which claimed to provide adequate protection with a final vaccination at 10 weeks of age, were compared. groups of 19 and 20 puppies with similar titres of maternally derived antibodies against canine parvovirus (cpv), canine distemper virus (cdv) and canine adenovirus type 2 (cav-2) at four weeks of age were vaccinated at six and 10 weeks of age and their responses to each vaccination were measured by comparing t ... | 2006 | 17127756 |
| a single immunization with a recombinant canine adenovirus expressing the rabies virus g protein confers protective immunity against rabies in mice. | rabies vaccines based on live attenuated rabies viruses or recombinant pox viruses expressing the rabies virus (rv) glycoprotein (g) hold the greatest promise of safety and efficacy, particularly for oral immunization of wildlife. however, while these vaccines induce protective immunity in foxes, they are less effective in other animals, and safety concerns have been raised for some of these vaccines. because canine adenovirus 2 (cav2) is licensed for use as a live vaccine for dogs and has an ex ... | 2006 | 16938327 |
| three-year rabies duration of immunity in dogs following vaccination with a core combination vaccine against canine distemper virus, canine adenovirus type-1, canine parvovirus, and rabies virus. | thirty-two seronegative pups were vaccinated at 8 weeks of age with modified-live canine distemper virus (cdv), canine adenovirus type-2 (cav-2), and canine parvovirus (cpv) vaccine and at 12 weeks with a modified-live cdv, cav-2, cpv, and killed rabies virus vaccine. an additional 31 seronegative pups served as age-matched, nonvaccinated controls. all test dogs were strictly isolated for 3 years after receiving the second vaccination and then were challenged with virulent rabies virus. clinical ... | 2006 | 17039445 |
| administration of a conditionally replicative oncolytic canine adenovirus in normal dogs. | conditionally replicative adenoviruses (crads) are engineered to replicate only in the target tissue and destroy tumor through their cytopathic effect. because of restricted in vivo replication, it is difficult to model behavior of human ad5-based vectors in animal subjects. to circumvent this, we developed a "syngeneic" canine crad based on canine adenovirus type 2 (cav2) transcriptionally targeted to canine osteosarcoma (os) cells. canine os is an outstanding model of human os and is the most ... | 2006 | 17257075 |
| expression of the foot-and-mouth disease virus vp1 protein using a replication-competent recombinant canine adenovirus type 2 elicits a humoral antibody response in a porcine model. | to develop a new type vaccine for foot-and-mouth disease (fmd) prevention by using canine adenovirus as vector, the vp1 cdna of foot-and-mouth disease virus (fmdv) type o strain china 99 was amplified by rt-pcr and cloned into pegfp-c1 by replacing the gfp gene with the vp1 cdna, resulting in an expression plasmid pvp1-c1. the expression cassette of vp1 composed of the cmv promoter, the vp1 gene and the sv40 early mrna polyadenylation signal was recovered by nsi i / mlu i digestion of pvp1-c1 an ... | 2006 | 16817763 |
| structural and mutational analysis of human ad37 and canine adenovirus 2 fiber heads in complex with the d1 domain of coxsackie and adenovirus receptor. | adenovirus fibers from most serotypes bind the d1 domain of coxsackie and adenovirus receptor (car), although the binding residues are not strictly conserved. to understand this further, we determined the crystal structures of canine adenovirus serotype 2 (cav-2) and the human adenovirus serotype 37 (had37) in complex with human car d1 at 2.3 and 1.5a resolution, respectively. structure comparison with the had12 fiber head-car d1 complex showed that the overall topology of the interaction is con ... | 2006 | 16923808 |
| the conundrum between immunological memory to adenovirus and their use as vectors in clinical gene therapy. | in the context of clinical gene transfer using viral vectors, the risk of memory antivector immunity is often poorly appreciated. the immunological past of the patient, the site of injection, and the vector dose will play intertwined and decisive roles in the safety and efficacy of treatment. to circumvent the drawbacks due to the ubiquitous human adenovirus (had) memory immunity, we believe that vectors derived from canine adenovirus type 2 (cav-2) will be more clinically useful than those deri ... | 2006 | 17172670 |
| pathogen exposure in endangered island fox (urocyon littoralis) populations: implications for conservation management. | island fox (urocyon littoralis) populations on four california channel islands have declined severely since 1994. canine distemper (cdv) was suspected to be responsible for the decline of the santa catalina island fox, so knowledge of infectious disease exposure in the remaining island fox populations was urgently needed. this study reviewed previous pathogen exposure in island foxes and investigated the current threat by conducting a serologic survey of foxes on all islands and sympatric feral ... | 2006 | 32226080 |
| [construction and identification of recombinant canine adenovirus type 2 expressing exogenous rabies glycoprotein (rgp)]. | safe and effective vaccination is important for rabies prevention. here, genetically engineered rabies vaccine cav2-deltae3-rgp was developed and characterized. the recombinant genome ppoly2-cav2-deltae3-rgp carrying the rabies glycoprotein (rgp) cdna was generated by a series of strictly gene cloning steps and infectious recombinant virus cav2-deltae3-rgp was obtained by transfecting the recombinant genome into a canine kidney cell line, mdck. to efficiently construct cloned recombinant canine ... | 2007 | 17552245 |
| experimental immunization of cats with a recombinant rabies-canine adenovirus vaccine elicits a long-lasting neutralizing antibody response against rabies. | during the past decade, human rabies caused by cats has ranked the second highest in china. several recombinant rabies vaccines have been developed for dogs. however, seldom have these vaccines been assessed or used in cats. in this trial, we report the experimental immunization of a recombinant canine adenovirus-rabies vaccine, cav-2-e3delta-rgp, in cats. thirty cats were inoculated with the recombinant vaccine intramuscularly, orally and intranasally, respectively. safety and efficacy studies ... | 2007 | 17576027 |
| simultaneous canine distemper virus, canine adenovirus type 2, and mycoplasma cynos infection in a dog with pneumonia. | the present case is the first description of a triple infection with canine distemper virus (cdv), canine adenovirus (cav) type 2, and mycoplasma cynos in a dog. the 5-month-old female miniature pinscher was euthanized because of dyspnea, croaking lung sounds, weight loss, and lymphopenia. pathologic examination revealed a fibrinous necrotizing pneumonia with large amphophilic intranuclear and acidophilic intracytoplasmatic inclusion bodies in different lung cells. immunohistochemically, cdv ant ... | 2007 | 17606512 |
| canine adenovirus type 1 infection of a eurasian river otter (lutra lutra). | a 10-year-old female eurasian river otter (lutra lutra) died after prolonged anorexia and weight loss in the seoul grand park zoo, seoul, republic of korea. on necropsy, the liver was found to be swollen and friable with 1 lobe enlarged and necrotic. the other organs showed no significant alterations except for mild atrophy of the right kidney. microscopically, there was multifocal hepatic necrosis. the hepatocytes around the necrotic areas were swollen and contained large basophilic intranuclea ... | 2007 | 17606519 |
| contrasting effects of human, canine, and hybrid adenovirus vectors on the phenotypical and functional maturation of human dendritic cells: implications for clinical efficacy. | antipathogen immune responses create a balance between immunity, tolerance, and immune evasion. however, during gene therapy most viral vectors are delivered in substantial doses and are incapable of expressing gene products that reduce the host's ability to detect transduced cells. gene transfer efficacy is also modified by the in vivo transduction of dendritic cells (dc), which notably increases the immunogenicity of virions and vector-encoded genes. in this study, we evaluated parameters that ... | 2007 | 17229706 |
| combined distemper-adenoviral pneumonia in a dog. | a 3 1/2-month-old pug with oculonasal discharge and seizures was submitted for postmortem examination. grossly, the lungs had cranioventral consolidation, and microscopically, 2 distinct types of inclusion bodies compatible with canine distemper virus and canine adenovirus type 2. presence of both viruses was confirmed via immunohistochemical staining. | 2007 | 17616064 |
| three-year serologic immunity against canine parvovirus type 2 and canine adenovirus type 2 in dogs vaccinated with a canine combination vaccine. | a group of client-owned dogs and a group of dogs at a commercial kennel were evaluated for duration of antibody responses against canine parvovirus type 2 (cpv-2) and canine adenovirus type 1 (cav-1) after receiving a combination vaccine containing recombinant canarypox-vectored canine distemper virus (cdv) and modified-live cpv-2, cav-2, and canine parainfluenza virus, with (c6) or without (c4) two serovars of leptospira (recombitek c4 or c6, merial). duration of antibody, which correlates with ... | 2007 | 18183549 |
| infectious disease monitoring of the endangered hawaiian monk seal. | as part of conservation efforts between 1997 and 2001, more than 25% (332 animals) of the endangered hawaiian monk seal (monachus schauinslandi) population was sampled in the northwestern hawaiian islands. serum samples were tested for antibodies to viruses, bacteria, and parasites known to cause morbidity and mortality in other marine mammal species. antibodies were found to phocine herpesvirus-1 by using an enzyme-linked immunosorbent assay, but seropositive results were not confirmed by virus ... | 2007 | 17495307 |
| canine respiratory viruses. | acute contagious respiratory disease (kennel cough) is commonly described in dogs worldwide. the disease appears to be multifactorial and a number of viral and bacterial pathogens have been reported as potential aetiological agents, including canine parainfluenza virus, canine adenovirus and bordetella bronchiseptica, as well as mycoplasmas, streptococcus equi subsp. zooepidemicus, canine herpesvirus and reovirus-1,-2 and -3. enhancement of pathogenicity by multiple infections can result in more ... | 2007 | 17296161 |
| infectious canine hepatitis: an "old" disease reemerging in italy. | four outbreaks of infectious canine hepatitis (ich) occurring in italy between 2001 and 2006 are reported. three outbreaks were observed in animal shelters of southern italy, whereas a fourth outbreak involved two purebred pups imported from hungary few days before the onset of clinical symptoms. in all outbreaks canine adenovirus type 1 (cav-1) was identified by virus isolation and pcr. in three outbreaks, other canine viral pathogens were detected, including canine distemper virus, canine parv ... | 2007 | 17197003 |
| compatibility of a bivalent modified-live vaccine against bordetella bronchiseptica and cpiv, and a trivalent modified-live vaccine against cpv, cdv and cav-2. | eight puppies (group 1) were vaccinated once with a bivalent modified-live vaccine against infectious tracheobronchitis by the intranasal route and at the same time with an injectable trivalent vaccine against canine parvovirus, canine distemper virus and canine adenovirus; a second group of eight puppies (group 2) was vaccinated only with the intranasal bivalent vaccine, and a further eight puppies (group 3) were vaccinated only with the injectable trivalent vaccine. three weeks later they were ... | 2007 | 17220520 |
| augmented humoral and cellular immune responses induced by canine adenovirus type 1 dna vaccine in balb/c mice. | infectious canine hepatitis (ich) is caused by canine adenovirus type 1 (cav-1), which severely harms infected animals. vaccination provides an effective approach to preventing canine infectious diseases. with the objective of exploring a new vaccination strategy that may prevent or cure ich, we constructed a dna vaccine, pvax1-cpg-loop, and evaluated its immune efficacy. we found that vaccination of balb/c mice with the dna vaccine alone, or priming with dna vaccine and boosting with the loop p ... | 2007 | 17931116 |
| infectious canine hepatitis in a gray fox (urocyon cinereoargenteus). | a free-ranging adult male gray fox (urocyon cinereoargenteus) with moderate diarrhea and thick ocular mucus discharge was examined postmortem. microscopically, the fox had intranuclear inclusion bodies within hepatocytes. canine adenovirus-1 was identified by polymerase chain reaction (pcr) and nucleotide sequencing. to our knowledge, this is the first report of clinical infectious canine hepatitis in a gray fox. | 2007 | 17984271 |
| a serologic assessment of exposure to viral pathogens and leptospira in an urban raccoon (procyon lotor) population inhabiting a large zoological park. | in urban environments, raccoons (procyon lotor) may act as reservoirs for an array of pathogenic organisms, presenting spillover risks for human, domestic animal, and captive (zoo) animal populations. over 5 yr, 159 raccoons from a high-density raccoon population in st. louis, missouri (usa), were surveyed for exposure to canine distemper virus (cdv), canine adenovirus 1 (cav-1); feline parvovirus (fpv; =feline panleukopenia), and several serovars of leptospira interrogans. exposure to each of t ... | 2007 | 17469271 |
| serosurvey of ex situ giant pandas (ailuropoda melanoleuca) and red pandas (ailurus fulgens) in china with implications for species conservation. | conservation strategies for the giant panda (ailuropoda melanoleuca) include the development of a self-sustaining ex situ population. this study examined the potential significance of infectious pathogens in giant pandas ex situ. serologic antibody titers against canine distemper virus (cdv), canine parvovirus (cpv), canine adenovirus (cav), canine coronavirus (ccv), canine herpesvirus, canine parainfluenza virus (cpiv), toxoplasma gondii, neospora caninum, and leptospira interrogans were measur ... | 2007 | 18229861 |
| [construction of a transfer vector based on canine adenovirus type-2]. | canine adenovirus type 2 (cav-2) has been proposed as a vector for recombinant vaccine. alternatively, it may be an attractive tool for gene transfer due to lack of pre-existing immunity in humans. in this study, a transfer vector based on cav-2, in which the 1381bp fragment of the e3 region was deleted, and a linker containing the not i, cla i, fse i restriction enzyme sites were cloned into the deleted region. the recombinant cav-2 genome was released from the plasmids enzyme digestion and tra ... | 2007 | 17460909 |
| fiber-knob modifications enhance adenoviral tropism and gene transfer in malignant glioma. | malignant gliomas remain refractory to ad5-mediated gene therapy due to deficiency of the coxsackie adenovirus receptor on tumor cells. the purpose of this study was to evaluate whether changes in adenoviral tropism can enhance gene transfer in the context of malignant glioma. | 2007 | 17351980 |
| serosurvey of infectious disease agents of carnivores in captive red pandas (ailurus fulgens) in china. | the future of the endangered red panda (ailurusfulgens) depends in part on the development of protective measures against infectious diseases. the present study is a first step toward improved understanding of infectious diseases in the species' home regions. serum samples obtained from 73 red pandas in 10 captive facilities in southwest, east, and northeast china from october to december 2004 were tested for antibodies against nine common infectious pathogens of carnivores. antibody titers agai ... | 2007 | 17469274 |
| oral vaccination of dogs (canis familiaris) with baits containing the recombinant rabies-canine adenovirus type-2 vaccine confers long-lasting immunity against rabies. | rabies is a reemerging and fatal infectious disease in asia mainly caused by exposure to rabid dogs. prevention of dog rabies would be the most effective way to stop rabies transmission to humans. however, vaccinating stray dogs in urban and rural areas using conventional vaccines is always difficult and is not cost-effective for use in most areas including china. further to previous studies from our laboratory, we developed a bait containing the recombinant rabies vaccine and performed a non-pa ... | 2008 | 18083277 |
| complete protection of cats against feline panleukopenia virus challenge by a recombinant canine adenovirus type 2 expressing vp2 from fpv. | feline panleukopenia virus (fpv) is an important infectious pathogen of all members of the family felidae. here, we describe construction of a replication-competent recombinant canine adenovirus type 2 (cav-2) expressing the vp2 protein of fpv (cav-2-vp2) by transfection of mdck cells with recombinant cav-2 genome carrying a vp2 expression cassette. ten 3-month-old cats were vaccinated with the recombinant virus with two boosters at 15-day intervals. all cats developed neutralizing antibodies of ... | 2008 | 18313810 |
| detection of canine adenoviral infections in urine and faeces by the polymerase chain reaction. | the present study was aimed at developing the polymerase chain reaction (pcr) assay for detection of canine adenoviruses from faecal or urine samples. urine or faecal samples were treated with chloroform or activated charcoal to eliminate the pcr inhibitory substances and the total dna was extracted. the pcr was optimized using common set of primers to amplify 508 bp or 1,030 bp dna sequence within e3 gene of canine adenovirus-1 (cav-1) and canine adenovirus-2 (cav-2), respectively. the pcr assa ... | 2008 | 18329729 |
| assessment of serum antibody titers against canine distemper virus, canine adenovirus type ii, and canine parvovirus in alaskan sled dogs before and after a long-distance race. | to determine serum antibody titers against canine distemper virus (cdv), canine adenovirus type ii (cav-2), and canine parvovirus (cpv) in trained sled dogs prior to and after completion of a long-distance race. | 2008 | 18518808 |
| enhanced gene delivery to human primary endothelial cells using tropism-modified adenovirus vectors. | endothelial cells have been noted to have relatively low expression of the native receptor for adenovirus serotype 5 (ad5), coxsackie and adenovirus receptor (car), and are thus refractory to ad5 infection. in this study, we hypothesize that increases in the infectivity of ad5 in primary human pulmonary artery (hpaec), coronary artery (hcaec) and umbilical vein endothelial cells (huvec) can be achieved through genetic capsid modification of ad5 to bypass car-dependent infection. the modification ... | 2008 | 19834585 |
| etiologic study of upper respiratory infections of household dogs. | infectious tracheobronchitis (itb), also known as the kennel cough, is a respiratory syndrome of dogs and usually appears to be contagious among dogs housed in groups. etiologic agent of itb is multiple and sometimes complex. in the present study, 68 household dogs showing clinical signs of respiratory infection were examined, and 20 dogs (29.4%) were found to be positive for either of following agents. bordetella bronchiseptica (b.b.) was most frequently detected from nasal and oropharynx sites ... | 2008 | 18628596 |
| canine adenovirus based rabies vaccines. | adenovirus based vectors are very attractive candidates for vaccination purposes as they induce in mammalian hosts potent humoral, mucosal and cellular immune responses to antigens encoded by the inserted genes. we have generated e1-deleted and replication-competent recombinant canine type-2 adenoviruses expressing the rabies virus glycoprotein (g). the effectiveness of both vectors to express a native g protein has been characterized in vitro in permissive cell lines. we compared the humoral an ... | 2008 | 18634509 |
| efficacy and safety of a live canine adenovirus-vectored rabies virus vaccine in swine. | rabies infections in swine have been reported occasionally in recent years in certain geographic locations. although a protective vaccine consisting of inactivated rabies virus is available for use in swine, searching for a more economically viable formulation for use in developing countries is always a priority. this work describes the testing of a canine adenovirus that expresses a rabies viral epitope (cav-2-e3delta-rgp) in a porcine rabies model. the data presented here show that the recombi ... | 2008 | 18721839 |
| development of recombinant canine adenovirus type-2 expressing the gn glycoprotein of seoul virus. | seoul virus glycoprotein gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. in this study, a replication-competent recombinant canine adenovirus type-2 expressing gn was constructed by the in vitro ligation method. the gn expression cassette, including the human cytomegalovirus (hcmv) promoter/enhancer and the sv40 early mrna polyadenylation signal, was cloned into the sspi site of the e3 region which is not ess ... | 2008 | 18249007 |
| immune-mediated canine and feline keratitis. | although the normal cornea is devoid of vasculature and lymphatics, there are still several immune-mediated corneal conditions that can occur in dogs and cats. an overview of corneal immunology is presented. diseases of dogs, including chronic superficial keratitis, superficial punctate keratitis, and canine adenovirus endotheliitis, as well as feline diseases, including eosinophilic keratitis and herpesvirus-related conditions, are discussed. | 2008 | 18299007 |
| three-dimensional structure of canine adenovirus serotype 2 capsid. | there are more than 100 known adenovirus (adv) serotypes, including 50 human serotypes. because adv-induced disease is relatively species specific, vectors derived from nonhuman serotypes may have wider clinical potential based, in part, on the lack of ubiquitous memory immunity. whereas a few of the human serotype capsids have been studied at the structural level, none of the nonhuman serotypes has been analyzed. the basis laid by the analysis of human adv (hadv) has allowed us to determine and ... | 2008 | 18216088 |
| canine adenoviruses and herpesvirus. | canine adenoviruses (cavs) and canine herpesvirus (chv) are pathogens of dogs that have been known for several decades. the two distinct types of cavs, type 1 and type 2, are responsible for infectious canine hepatitis and infectious tracheobronchitis, respectively. in the present article, the currently available literature on cavs and chv is reviewed, providing a meaningful update on the epidemiologic, pathogenetic, clinical, diagnostic, and prophylactic aspects of the infections caused by thes ... | 2008 | 18501279 |
| serologic survey for selected viral pathogens in free-ranging endangered european mink (mustela lutreola) and other mustelids from south-western france. | to investigate the possible role of selected pathogens in the decline of endangered european mink (mustela lutreola) populations and the potential for these pathogens to affect mink survival, a serologic survey was conducted using serum samples collected from march 1996 to march 2003 in eight departments of south-western france. in total, 481 free-ranging individuals of five mustelid species (including the european mink) were tested. sympatric mustelids can serve as sentinels to determine the pr ... | 2008 | 18957635 |
| tyzzer's disease in a eurasian otter (lutra lutra) in scotland. | a eurasian otter (lutra lutra) cub found in weak condition on the isle of harris, scotland, developed bilateral corneal oedema 16 days after being admitted to a rehabilitation centre. it died unexpectedly on day 26. on postmortem examination, there was excess clear fluid in the body cavities and the liver was swollen with numerous pale focal lesions and petechial haemorrhages throughout. histopathological examination revealed bundles of bacilli morphologically typical of clostridium piliforme wi ... | 2008 | 18978367 |
| demonstration of 1-year duration of immunity for attenuated bordetella bronchiseptica vaccines in dogs. | three groups of healthy dogs with low antibody titers to bordetella bronchiseptica (bb), canine parainfluenza virus (cpi), and canine adenovirus type 2 (cav-2) were used in this study. one group was vaccinated with a single dose of monovalent attenuated bb vaccine and one group with a trivalent vaccine containing attenuated bb, cpi, and cav-2; dogs were vaccinated intranasally with a single dose of the respective vaccines. the third group served as unvaccinated controls. all vaccinated dogs subs ... | 2008 | 19177330 |
| functional organization of the major late transcriptional unit of canine adenovirus type 2. | vectors derived from canine adenovirus type 2 (cav-2) are attractive candidates for gene therapy and live recombinant vaccines. cav-2 vectors described thus far have been generated by modifying the virus genome, most notably early regions 1 and 3 or the fiber gene. modification of these genes was underpinned by previous descriptions of their mrna and protein-coding sequences. similarly, the construction of new cav-2 vectors bearing changes in other genomic regions, in particular many of those ex ... | 2009 | 19264594 |
| a single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus gn glycoprotein confers protective immunity against seoul virus in mice. | seoul virus (seov), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (hfrs) and afflicts tens of thousands of people annually. in this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gn protein of seov (rcav-2-gn) in balb/c mice. sera from immunized mice contained neutralizing antibodies that could specifically recognize seov and neutralize its infectivity in vitro. ... | 2009 | 19583957 |
| gag-specific immune enhancement of lentiviral infection after vaccination with an adenoviral vector in an animal model of aids. | the evaluation of vaccine strategies in animal models is essential for the development of a vaccine against hiv. in efficacy trials conducted in non-human primate models of aids, vaccines based on adenoviruses compared favourably with other vaccine vectors. to determine whether this strategy could be transposed to another animal model, and by extension, to humans, we have evaluated the efficacy of adenoviral vectors in a natural model of aids, infection of the cat by the feline immunodeficiency ... | 2009 | 19070641 |
| hematology, parasitology, and serology of free-ranging coyotes (canis latrans) from south carolina. | blood and feces were collected from 34 adult (19 males, 15 females) and seven juvenile (three males, one female, three not reported) free-ranging coyotes (canis latrans) on the us department of energy's savannah river site (south carolina, usa). significant (p<0.05) hematologic differences by sex were noted for red blood cell counts, hemoglobin, and hematocrit. biochemical differences by sex occurred only for albumen (p<0.05). twenty-one adults were antibody positive for at least one of four vir ... | 2009 | 19617502 |
| virologic survey of dogs with naturally acquired idiopathic conjunctivitis. | to determine the frequency of viral detection in conjunctival samples from client-owned domestic dogs with naturally acquired idiopathic conjunctivitis and to identify signalment, historical, and clinical findings positively associated with viral detection. | 2009 | 19827980 |
| a retrospective investigation of canine adenovirus (cav) infection in adult dogs in turkey. | canine adenovirus (cav) type 1 and 2, respectively, cause infectious canine hepatitis and infectious canine laryngotracheitis in members of the families canidae and ursidae worldwide. both of these infections are acute diseases, especially in young dogs. the aim of this study was to conduct a serological investigation of canine adenovirus infection. for this purpose, serum samples were collected from native pure-bred kangal(n = 11), and akbash dogs (n = 17) and turkish greyhounds (n = 15) in esk ... | 2009 | 19831268 |
| oral immunization of raccoons and skunks with a canine adenovirus recombinant rabies vaccine. | oral vaccination is an important part of wildlife rabies control programs. currently, the vaccinia-rabies glycoprotein recombinant virus is the only oral rabies vaccine licensed in the united states, and it is not effective in skunks. in the current study, captive raccoons and skunks were used to evaluate a vaccine developed by incorporating the rabies virus glycoprotein gene into a canine adenovirus serotype 2 vector (cav2-rvg). seven of 7 raccoons orally vaccinated with cav2-rvg developed viru ... | 2009 | 19925952 |
| a serological survey of infectious disease in yellowstone national park's canid community. | gray wolves (canis lupus) were reintroduced into yellowstone national park (ynp) after a >70 year absence, and as part of recovery efforts, the population has been closely monitored. in 1999 and 2005, pup survival was significantly reduced, suggestive of disease outbreaks. | 2009 | 19756151 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| an update on canine adenovirus type 2 and its vectors. | adenovirus vectors have significant potential for long- or short-term gene transfer. preclinical and clinical studies using human derived adenoviruses (had) have demonstrated the feasibility of flexible hybrid vector designs, robust expression and induction of protective immunity. however, clinical use of had vectors can, under some conditions, be limited by pre-existing vector immunity. pre-existing humoral and cellular anti-capsid immunity limits the efficacy and duration of transgene expressi ... | 2010 | 21994722 |
| serosurvey of selected viruses in captive giant pandas (ailuropoda melanoleuca) in china. | serum samples from 92 giant pandas in three captive facilities were tested for antibodies against five viruses of carnivores. antibody titers against canine distemper virus (cdv) in two facilities in which giant pandas were vaccinated were variable. the canine adenovirus (cav-1) and canine parvovirus (cpv) titers in vaccinated group were both positive, but titers were not high and varied among individual except one vaccinated panda had extremely high cav-1 titer, indicating infection with the fi ... | 2010 | 19913371 |
| sgsh gene transfer in mucopolysaccharidosis type iiia mice using canine adenovirus vectors. | many viral backbones have been used as gene transfer vectors. however, the efficacy of therapy based on human-derived vectors may be limited by the high incidence of pre-existing humoral and cellular memory immunity. to circumvent some of the clinical disadvantages of vectors derived from common human pathogens, we have used an e1-deleted vector derived from a xenogenic adenovirus, canine adenovirus serotype 2 (cav-2) to ameliorate neuropathological changes associated with the lysosomal storage ... | 2010 | 20231109 |
| a multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus. | a multiplex reverse transcription-nested polymerase chain reaction (rt-npcr) method was developed for the detection and differentiation of wild-type and vaccine strains of canine distemper virus (cdv). a pair of primers (p1 and p4) specific for cdv corresponding to the highly conserved region of the cdv genome were used as a common primer pair in the first-round pcr of the nested pcr. primers p2 specific for cdv wild-type strains, were used as the forward primer together with the common reverse ... | 2010 | 20433759 |
| evaluation of the antiviral activity of chlorine dioxide and sodium hypochlorite against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. | we evaluated the antiviral activity of a chlorine dioxide gas solution (cd) and sodium hypochlorite (sh) against feline calicivirus, human influenza virus, measles virus, canine distemper virus, human herpesvirus, human adenovirus, canine adenovirus and canine parvovirus. cd at concentrations ranging from 1 to 100 ppm produced potent antiviral activity, inactivating >or= 99.9% of the viruses with a 15 sec treatment for sensitization. the antiviral activity of cd was approximately 10 times higher ... | 2010 | 20616431 |
| [infectious canine hepatitis in 4 dogs in switzerland]. | infectious canine hepatitis in 4 dogs in switzerland. four dogs presented with nonspecific symptoms of lethargy, vomiting, diarrhea, fever and weakness. laboratory results were consistent with hepatopathy and disseminated intravascular coagulation. three dogs died, one survived. in the three deceased dogs, a diagnosis of infectious canine hepatitis (ich) was made based on histological findings and positive immunhistochemistry results for canine adenovirus-1 (cav-1). in the surviving dog, an ante ... | 2010 | 20127647 |
| development and validation of a real-time pcr assay for specific and sensitive detection of canid herpesvirus 1. | a taqman-based real-time pcr assay targeting the glycoprotein b-encoding gene was developed for diagnosis of canid herpesvirus 1 (chv-1) infection. the established assay was highly specific, since no cross-reactions were observed with other canine dna viruses, including canine parvovirus type 2, canine minute virus, or canine adenovirus types 1 and 2. the detection limit was 10(1) and 1.20 x 10(1) dna copies per 10 microl(-1) of template for standard dna and a chv-1-positive kidney sample, respe ... | 2010 | 20674611 |
| age and long-term protective immunity in dogs and cats. | vaccination can provide an immune response that is similar in duration to that following a natural infection. in general, adaptive immunity to viruses develops earliest and is highly effective. such anti-viral immune responses often result in the development of sterile immunity and the duration of immunity (doi) is often lifelong. in contrast, adaptive immunity to bacteria, fungi or parasites develops more slowly and the doi is generally short compared with most systemic viral infections. steril ... | 2010 | 19959181 |
| generation of e3-deleted canine adenovirus type 2 expressing the gc glycoprotein of seoul virus by gene insertion or deletion of related terminal region sequences. | seoul virus (seov) is one of the four hantaviruses known to cause haemorrhagic fever with renal syndrome. the medium genome segment encodes the gn/gc glycoproteins of seov, which form the major structural part of the virus envelope. gc and/or gn are the candidate antigens of hantavirus for induction of a highly immunogenic response for hantavirus vaccine. in this study, the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the gc protein of seov w ... | 2010 | 20181748 |
| serosurvey for canine distemper virus, canine adenovirus, leptospira interrogans, and toxoplasma gondii in free-ranging canids in scandinavia and svalbard. | prevalence of antibodies reactive to canine distemper virus (cdv), canine adenovirus type 1 (cav-1), leptospira interrogans serovars canicola and icterohaemorrhagiae, and toxoplasma gondii were examined in free-ranging scandinavian canids. sampling included 275 red foxes (vulpes vulpes) from mainland norway, 60 arctic foxes (vulpes lagopus) from the high-arctic islands of svalbard, and 98 wolves (canis lupus) from the joint swedish-norwegian population. methods used included virus neutralization ... | 2010 | 20688639 |
| immune responses in pigs induced by recombinant canine adenovirus 2 expressing the glycoprotein 5 of porcine reproductive and respiratory syndrome virus. | to develop a new type vaccine for porcine reproductive and respiratory syndrome (prrs) prevention by using canine adenovirus 2(cav-2) as vector, the glycoprotein 5(gp5) gene from prrsv strain jl was amplified by rt-pcr, and the expression cassette of gp5 was constructed using the human cytomegalovirus (hcmv) promoter and the simian virus 40 (sv40) early mrna polyadenylation signal. the expression cassette of glycoprotein 5 was cloned into the cav-2 genome in which e3 region had been partly delet ... | 2010 | 20432066 |
| construction and characterization of a recombinant canine adenovirus expressing gp5 and m proteins of porcine reproductive and respiratory syndrome virus. | the causative agent of porcine reproductive and respiratory syndrome (prrs) is prrs virus (prrsv), which belongs to the family arteriviridae. gp5/m protein complex of prrsv binds to sialoadhesion expressed on the cells to infect the cells. in this study, we developed a canine adenovirus type 2 (cav-2) recombinant, termed rcav2-gp5/m, expressing gp5 and m proteins. to evaluate the immunogenicity of the recombinant virus, mice were inoculated subcutaneously with rcav2-gp5/m, and specific antibodie ... | 2010 | 20467206 |
| fatal canine adenoviral pneumonia in two litters of bulldogs. | five bulldog pups, 4 weeks of age or younger, were presented over a 2-day period for postmortem examination and diagnostic evaluation. the pups originated from 2 different litters but had been cared for at a common facility since their birth. all 5 pups died after exhibiting symptoms consisting of lethargy, dyspnea, nasal discharge, anorexia, vomiting, diarrhea, and abdominal pain. necropsy examination revealed locally extensive to diffusely red, firm, consolidated lungs in all pups. histopathol ... | 2010 | 20807943 |
| osteosarcoma cells as carriers to allow antitumor activity of canine oncolytic adenovirus in the presence of neutralizing antibodies. | osteosarcoma (osa) is the most common bone tumor affecting the dog. the veterinary options for therapeutic management of osa are limited and prognosis for such patients is poor. oncolytic adenoviruses are attractive tools for experimental therapeutics as they can replicate and spread within tumors to directly induce tumor destruction. however, a major impediment to systemic oncolytic adenoviruses injection is the presence of pre-existing neutralizing antibodies (nabs). in this study, we investig ... | 2010 | 20671769 |
| canine adenoviruses elicit both humoral and cell-mediated immune responses against rabies following immunisation of sheep. | safe and efficient vaccination is important for rabies prevention in domestic animals. replicative vectors expressing the rabies virus glycoprotein, derived from canine adenovirus have been reported to be promising vaccines in various animal models. in this paper we compare the potential of a replicative and a non-replicative vector, both based on canine adenovirus type 2 and expressing the rabies glycoprotein. upon inoculation in sheep, immune responses against the rabies virus protein elicited ... | 2010 | 21134446 |
| lights and shades on an historical vaccine canine distemper virus, the rockborn strain. | both egg- and cell-adapted canine distemper virus (cdv) vaccines are suspected to retain residual virulence, especially if administered to immuno-suppressed animals, very young pups or to highly susceptible animal species. in the early 1980s, post-vaccine encephalitis was reported in dogs from various parts of britain after administration of a particular batch of combined cdv rockborn strain/canine adenovirus type-1 vaccine, although incrimination of the rockborn strain was subsequently retracte ... | 2010 | 21167117 |
| an endocytic carriage tale: adenoviruses internalization and trafficking in neurons. | in immune-competent hosts, adenoviruses (ads) are mild pathogens that cause mainly infections of the respiratory and ocular tracks. the advent of ad-based gene transfer vectors made the understanding of their interaction with the host cellular machinery an intensive field of research over the last decade. as studies focused primarily on epithelial-like cells, the mechanism of neuronal uptake of ads was still missing. using a combination of biochemical and cell biology approaches, we characterize ... | 2010 | 21178440 |
| antibodies to parvovirus, distemper virus and adenovirus conferred to household dogs using commercial combination vaccines containing leptospira bacterin. | to examine how the inclusion (+) or exclusion (-) of inactivated leptospira antigens in a vaccine for canine parvovirus type 2 (cpv-2), canine distemper virus (cdv) and canine adenovirus type 2 (cadv-2) affects antibody titres to cpv-2, cdv and cadv-1 antigens, household dogs were vaccinated with commercially available vaccines from one of three manufacturers. cpv-2, cdv and cadv-1 antibody titres were measured 11 to 13 months later and compared within three different age groups and three differ ... | 2010 | 21262693 |
| a real-time pcr assay for quantification of canine adenoviral vectors. | the pre-existing humoral and cellular immunity found in the great majority of the population raises concerns about the clinical efficacy and safety of vectors derived from ubiquitous human adenovirus serotypes. to alleviate these concerns, canine adenovirus type 2 vectors (cav-2) were developed. owing to their extraordinary neuronal tropism and efficient axonal retrograde transport, cav-2 vectors hold great promise for the treatment of neurodegenerative diseases. the development and validation o ... | 2010 | 19751766 |
| infectious canine hepatitis in red foxes (vulpes vulpes) in the united kingdom. | the pathological findings are described in three cases of infectious canine hepatitis in free-ranging red foxes (vulpes vulpes) in england. the foxes died after short periods of clinical illness. mild jaundice and hepatic congestion were evident grossly. on histopathological examination, intranuclear inclusion bodies were visible in hepatocytes, in association with hepatocyte dissociation and necrosis, as well as in renal glomeruli, renal tubular epithelial cells and vascular endothelial cells. ... | 2010 | 20097890 |
| wild canids, domestic dogs and their pathogens in southeast brazil: disease threats for canid conservation. | wild canids are under many pressures, including habitat loss, fragmentation and disease. the current lack of information on the status of wildlife health may hamper conservation efforts in brazil. in this paper, we examined the prevalence of canine pathogens in 21 free-ranging wild canids, comprising 12 cerdocyon thous (crab-eating fox), 7 chrysocyon brachyurus (maned wolf), 2 lycalopex vetulus (hoary fox), and 70 non-vaccinated domestic dogs from the serra do cipó national park area, southeast ... | 2010 | 32214695 |
| novel adenoviruses and herpesviruses detected in bats. | samples from native hungarian or captive bats were tested by pcr for the presence of adenoviruses and herpesviruses. two novel adenoviruses from a common noctule (nyctalus noctula) and a greater horseshoe (rhinolophus ferrum-equinum) bat were detected. in captive egyptian fruit bats (rousettus aegyptiacus), dna from two novel herpesviruses was demonstrated. phylogenetic analysis facilitated provisional taxonomic placement of the newly detected viruses. such analysis and the existence of unique, ... | 2011 | 20813566 |
| pharmacovigilance in veterinary medicine in chile: a pilot study. | irag++en, d., urcelay, s., san mart+¡n, b. pharmacovigilance in veterinary medicine in chile: a pilot study. j. vet. pharmacol. therap.34, 108-115. in chile, there is no present government policy to survey and analyse adverse drug reactions (adrs) in the field of veterinary medicine. the intent of this study is to assess, for the first time, adr frequency in treated animals. to this purpose, a 6-month period pilot study based on who recommendations was conducted to monitor adrs in cats and dogs ... | 2011 | 21395600 |
| Genome Analysis of Bat Adenovirus 2: Indications of Interspecies Transmission. | The genome of bat adenovirus 2 was sequenced and analyzed. It is similar in size (31,616 bp) to the genomes of bat adenovirus 3 and canine adenoviruses 1 and 2. These four viruses are monophyletic and share an identical genome organization, with one E3 gene and four E4 genes unique to this group among the mastadenoviruses. These findings suggest that canine adenoviruses may have originated by interspecies transfer of a vespertilionid bat adenovirus. | 2011 | 22130531 |
| isolation of a novel adenovirus from california sea lions zalophus californianus. | viral hepatitis associated with adenoviral infection has been reported in california sea lions zalophus californianus admitted to rehabilitation centers along the california coast since the 1970s. canine adenovirus 1 (cadv-1) causes viral hepatitis in dogs and infects a number of wildlife species. attempts to isolate the virus from previous sea lion hepatitis cases were unsuccessful, but as the hepatitis had morphologic features resembling canine infectious hepatitis, and since the virus has a w ... | 2011 | 21790072 |
| chronic hepatitis in the english springer spaniel: clinical presentation, histological description and outcome. | medical records and liver histology of 68 english springer spaniels (ess) with a histological diagnosis of ch were reviewed retrospectively. pcr was performed on liver tissue for canine adenovirus-1 (cav-1), canine parvovirus, canine herpesvirus and pathogenic leptospira species. follow-up information was obtained to calculate survival times. median age at presentation was three years seven months (range, seven months to eight years five months) and there were 48 female and 20 male dogs. clinica ... | 2011 | 21852307 |
| plaque purification as a method to mitigate the risk of adventitious-agent contamination in influenza vaccine virus seeds. | at present, the seed viruses for the manufacture of licensed seasonal inactivated influenza vaccines in the united states are derived from primary egg isolates as a result of concerns associated with adventitious agents. according to the prevailing view, the passage of influenza viruses through eggs serves as a filtering step to remove potential contaminating viruses. we have investigated the feasibility of addressing adventitious-agent risk by subjecting influenza virus to a plaque-purification ... | 2011 | 21354480 |
| antibody titers for canine parvovirus type-2, canine distemper virus, and canine adenovirus type-1 in adult household dogs. | serum antibody titers for canine parvovirus type-2 (cpv-2), canine distemper virus (cdv) and canine adenovirus type-1 (cav-1) were investigated in 1031 healthy adult household dogs (2 to 18 years old) given an annual inoculation in the previous 11 to 13 months. the number of dogs retaining significant titers of antibodies against cpv-2, cdv, and cav-1 were 888 (86%), 744 (72%), and 732 (71%), respectively. there were no differences between males and females in antibody titers against the 3 virus ... | 2011 | 22379198 |
| pathogens of wild maned wolves (chrysocyon brachyurus) in brazil. | the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ... | 2012 | 23060508 |
| Helper-dependent canine adenovirus vector-mediated transgene expression in a neurodegenerative lysosomal storage disorder. | Mucopolysaccharidosis type IIIA (MPS-IIIA) is a severe neurodegenerative lysosomal storage disorder caused by a deficiency of N-sulfoglucosamine sulfohydrolase (SGSH) activity with subsequent accumulation of partially-degraded heparan sulfate and other glycolipids. In this study, we have evaluated a gene therapy approach using a helper-dependent canine adenovirus vector that expresses human SGSH as a means of delivering sustained transgene expression to the brain. Initial testing in a mixed neur ... | 2012 | 21963444 |
| effects of body weight on antibody titers against canine parvovirus type 2, canine distemper virus, and canine adenovirus type 1 in vaccinated domestic adult dogs. | the objective of this study was to determine whether post-vaccination antibody titers vary according to body weight in adult dogs. antibody titers against canine parvovirus type 2 (cpv-2), canine distemper virus (cdv), and canine adenovirus type 1 (cadv-1) were measured for 978 domestic adult dogs from 2 to 6 y of age. the dogs had been vaccinated approximately 12 mo earlier with a commercial combination vaccine. the dogs were divided into groups according to their weight. it was found that mean ... | 2012 | 23543958 |
| exposure to selected pathogens in to selected pathogens in geoffroy's cats and domestic carnivores from central argentina. | wild carnivores share a high percentage of parasites and viruses with closely related domestic carnivores. because of increased overlap and potential contact with domestic species, we conducted a retrospective serosurvey for 11 common carnivore pathogens in 40 geoffroy's cats (leopardus geoffroyi) sampled between 2000 and 2008 within or near two protected areas in central argentina (lihué calel national park, la pampa, and campos del tuyú national park, buenos aires), as well as five domestic ca ... | 2012 | 23060491 |
| enteritis in an alpaca (vicugna pacos) associated with a potentially novel adenovirus. | adenovirus-associated enteritis was diagnosed by histopathology of small intestine in a 2-year-old alpaca (vicugna pacos). electron microscopy confirmed intracytoplasmic and intranuclear adenoviral particles within enterocytes. nucleic acid was extracted from paraffin-embedded tissue sections, and a pan-adenovirus nested polymerase chain reaction (pcr) assay was employed to target a partial sequence of the polymerase gene. the pcr product (321 bp) was cloned and sequenced. comparison of the nucl ... | 2012 | 22786976 |
| canine recombinant adenovirus vector induces an immunogenicity-related gene expression profile in skin-migrated cd11b⁺ -type dcs. | gene expression profiling of the blood cell response induced early after vaccination has previously been demonstrated to predict the immunogenicity of vaccines. in this study, we evaluated whether the analysis of the gene expression profile of skin-migrated dendritic cells (dcs) could be informative for the in vitro prediction of immunogenicity of vaccine, using canine adenovirus serotype 2 (cav2) as vaccine vector. cav2 has been shown to induce immunity to transgenes in several species includin ... | 2012 | 23300693 |
| development of a combined canine distemper virus specific rt-pcr protocol for the differentiation of infected and vaccinated animals (diva) and genetic characterization of the hemagglutinin gene of seven chinese strains demonstrated in dogs. | a combined reverse-transcription polymerase chain reaction (rt-pcr) method was developed for the detection and differentiation of wild-type and vaccine strains of the canine distemper virus (cdv). a pair of primers (p1/p2) was used to detect both cdv wild-type strains and vaccines. another pair (p3/p4) was used to detect only cdv wild-type strains. a 335bp fragment was amplified from the genomic rna of the vaccine and wild-type strains. a 555bp fragment was amplified specifically from the genomi ... | 2012 | 22108430 |
| sequence-independent vidisca-454 technique to discover new viruses in canine livers. | in many mammals, viruses cause hepatitis. despite many efforts a specific virus responsible for canine idiopathic hepatitis has not been identified. the discovery of a viral etiology in canine hepatitis will promote the development of specific drugs and vaccines for the treatment of idiopathic hepatitis in dogs. the objective of this study was the application of the sequence-independent virus discovery cdna-amplified fragment length polymorphism (vidisca) technique combined with high through-put ... | 2012 | 22664180 |
| a novel recombinant peste des petits ruminants-canine adenovirus vaccine elicits long-lasting neutralizing antibody response against ppr in goats. | peste des petits ruminants (ppr) is a highly contagious infectious disease of goats, sheep and small wild ruminant species with high morbidity and mortality rates. the peste des petits ruminants virus (pprv) expresses a hemagglutinin (h) glycoprotein on its outer envelope that is crucial for viral attachment to host cells and represents a key antigen for inducing the host immune response. | 2012 | 22623990 |
| a siglec-like sialic-acid-binding motif revealed in an adenovirus capsid protein. | sialic-acid-binding immunoglobulin-like lectins (siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. to see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding proteins, we analyzed 127 structures with bound sialic acids found in the protein data bank database. of these, the canine adenovirus 2-fiber knob protein showed close local structural re ... | 2012 | 22522600 |
| 3d cultures of human neural progenitor cells: dopaminergic differentiation and genetic modification. [corrected]. | central nervous system (cns) disorders remain a formidable challenge for the development of efficient therapies. cell and gene therapy approaches are promising alternatives that can have a tremendous impact by treating the causes of the disease rather than the symptoms, providing specific targeting and prolonged duration of action. hampering translation of gene-based therapeutic treatments of neurodegenerative diseases from experimental to clinical gene therapy is the lack of valid and reliable ... | 2012 | 22433395 |
| chromatography purification of canine adenoviral vectors. | canine adenovirus vectors (cav2) are currently being evaluated for gene therapy, oncolytic virotherapy, and as vectors for recombinant vaccines. despite the need for increasing volumes of purified cav2 preparations for preclinical and clinical testing, their purification still relies on the use of conventional, scale-limited cscl ultracentrifugation techniques. a complete downstream processing strategy for cav2 vectors based on membrane filtration and chromatography is reported here. microfiltra ... | 2012 | 22799886 |
| hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (chrysocyon brachyurus). | a 15-yr-old, female, maned wolf (chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. further classification by polymerase chain reaction, immunohistochemical staining, v ... | 2012 | 22779244 |
| molecular analysis of a fragment of gene e1b 19k of canine adenovirus 2 (cav-2) isolated from dogs with symptoms of cough. | the aim of this study was to perform molecular analysis of canine adenovirus 2 (cav-2) e1b 19k gene fragment isolated from 20 dogs of various breeds (12 males and 8 females aged 1-9 years), with clinical symptoms of upper respiratory tract infections, from the lubelszczyzna region. nasal swabs were taken from dogs. dna of cav-2 was detected using the pcr method in 16 swabs. all pcr products were sequenced, and the obtained sequences were compared with each other and with the sequence of the e1b ... | 2012 | 23214360 |
| booster effect of canine distemper, canine parvovirus infection and infectious canine hepatitis combination vaccine in domesticated adult dogs. | domesticated adult dogs with antibody titer classified as below 'high' to one or more of canine distemper virus (cdv), canine parvovirus type-2 (cpv-2) and canine adenovirus type-1 (cadv-1) were then given an additional inoculation, and the effectiveness of this booster evaluated 2 months later. consequently, cdv and cadv-1 antibody titer experienced a significant increase, but the same effect was not observed in the antibody titer of cpv-2. these findings suggest that with additional inoculatio ... | 2012 | 22672137 |
| duration of serological response to canine parvovirus-type 2, canine distemper virus, canine adenovirus type 1 and canine parainfluenza virus in client-owned dogs in australia. | to determine whether client-owned dogs in australia, last vaccinated with canvac(®) vaccines containing canine parvovirus-type 2 (cpv-2), canine distemper virus (cdv), canine adenovirus type 2 (cav-2) ± canine parainfluenza virus (cpiv) at least 18 months ago, were seropositive or responded serologically to revaccination. | 2012 | 23186088 |
| high prevalence of antibodies against canine adenovirus (cav) type 2 in domestic dog populations in south africa precludes the use of cav-based recombinant rabies vaccines. | rabies in dogs can be controlled through mass vaccination. oral vaccination of domestic dogs would be useful in the developing world, where greater vaccination coverage is needed especially in inaccessible areas or places with large numbers of free-roaming dogs. from this perspective, recent research has focused on development of new recombinant vaccines that can be administered orally in a bait to be used as adjunct for parenteral vaccination. one such candidate, a recombinant canine adenovirus ... | 2013 | 23867013 |
| assessing demographic and epidemiologic parameters of rural dog populations in india during mass vaccination campaigns. | mass vaccination of dogs is a mainstay for efforts to control rabies and other viral pathogens. the success of such programs is a function of the ability to vaccinate sufficient proportions of animals to develop herd immunity. however, fully assessing success in reaching target vaccination-levels and in understanding the outcome of mass vaccination efforts is hindered if insufficient information is available on the demographics of dog populations and the prevalence of the targeted pathogens. whi ... | 2013 | 23664490 |
| concomitant canine distemper, infectious canine hepatitis, canine parvoviral enteritis, canine infectious tracheobronchitis, and toxoplasmosis in a puppy. | the concomitant infections of canine distemper virus (cdv), canine adenovirus a types 1 (cadv-1) and 2 (cadv-2), canine parvovirus type 2 (cpv-2), and toxoplasma gondii are described in a 43-day-old mixed-breed puppy. clinically, there were convulsions and blindness with spontaneous death; 14 siblings of this puppy, born to a 10-month-old dam, which was seropositive (titer: 1,024) for t. gondii, also died. necropsy revealed unilateral corneal edema (blue eye), depletion of intestinal lymphoid ti ... | 2013 | 23293164 |