the b cell repertoire in patients with systemic autoimmune diseases: analysis of epstein-barr virus (ebv)-inducible circulating precursors that produce autoantibodies against nuclear ribonucleoprotein (nrnp).peripheral blood b cells from patients with systemic autoimmune disease and healthy volunteers were immortalized using ebv and the frequencies of b cell precursors that produced immunoglobulin class-specific antibodies against anti-nrnp, a specific marker for mixed connective tissue disease, were assessed using limiting dilution analysis. the frequencies of ebv-induced b cell precursors that produced igg anti-nrnp were correlated closely with the serum titres of the corresponding autoantibodies, ...19921333924
inhibition by squalene of the tumor-promoting activity of 12-o-tetradecanoylphorbol-13-acetate in mouse-skin carcinogenesis.squalene inhibited the effect of tumor promoter 12-o-tetradecanoylphorbol-13-acetate (tpa), such as increased 32pi incorporation into phospholipids of hela cell membrane, induction of epstein-barr-virus early antigen in raji cell and induction of ornithine decarboxylase in mouse skin. squalene also markedly suppressed the promoting activity of tpa on skin carcinogenesis in 7,12-dimethylbenz[a]anthracene-initiated mice.19921334056
v delta 1+ subset of human gamma delta t cells responds to ligands expressed by ebv-infected burkitt lymphoma cells and transformed b lymphocytes.human gamma delta t cells of peripheral blood can be divided in two groups in terms of their tcr as well as their behavior upon in vitro stimulation. the major subset expresses the tcr v-segments v gamma 9 and v delta 2 and proliferates in response to ligands revealed by various microorganisms, and the cell line daudi in addition. the minor group is less homogenous on the gamma-chain but is almost completely identified by mab against the v delta 1 segment; there is no ligand known to promote gro ...19921334108
viral involvement in hodgkin's disease.the etiology of hodgkin's disease (hd) is unknown, but a growing body of evidence suggests that the epstein-barr virus (ebv) plays a role in a proportion of cases. clonal ebv genomes have been detected in affected tissues, and ebv has been localized to reed-sternberg (rs) cells, the putative malignant cells in hd. ebv latent genes, including the eber rnas and the latent membrane protein, lmp-1, are expressed by rs cells. these data suggest that ebv is playing a role in the pathogenesis of hd; ho ...19921334112
distribution and localization of epstein-barr virus subtypes a and b in aids-related lymphomas and lymphatic tissue of hiv-positive patients.tissue samples of 21 hiv-positive patients have been studied for the presence and distribution of epstein-barr virus (ebv) subtypes a and b. this was done by pcr, eber in situ hybridization, and immunohistochemical detection of ebv latent membrane protein (lmp) in aids-associated malignant lymphomas (16 cases) and lymphatic organs of patients without lymphoma (5 cases). eleven cases were considered to be ebv-positive, with type a in four, and type b virus in four other cases. in patients with ma ...19921334141
lymphoproliferative lesions in patients with common variable immunodeficiency syndrome.we reviewed our experience with 30 nodal and extranodal lymphoid lesions from 17 patients with common variable immunodeficiency (cvid). immunohistochemical studies were performed on biopsies from 15 patients, in situ hybridization for epstein-barr virus in nine cases, and gene rearrangement analysis on seven lesions. the biopsies were classified into four groups: malignant lymphoma (two cases); atypical lymphoid hyperplasia (eight cases); reactive lymphoid hyperplasia (14 cases); and chronic gra ...19921334378
epidemiology of nasopharyngeal and salivary gland carcinoma in greenland.from 1950 to 1989 one hundred and forty-four cases of either undifferentiated nasopharyngeal carcinoma (npc) or salivary gland carcinoma (sgc) were diagnosed in greenlanders, all born in greenland. the greenland sgc is an anaplastic carcinoma with histopathology and electronmicroscopic cytopathological alterations as found in undifferentiated npc. both npc and sgc from greenland and alaska are associated with epstein-barr virus infection. the incidence rate of npc based on newly diagnosed cases ...19921334414
epstein-barr virus bcrf1 gene product (viral interleukin 10) inhibits superoxide anion production by human monocytes.due to its similar biological activities to interleukin 10 (il-10), epstein-barr virus (ebv) bcrf1 gene product (viral il-10: vil-10) has recently been recognized as an analogue of authentic il-10. preincubation of human monocytes with vil-10, like human il-10, induced smaller amounts of interferon-gamma (ifn-gamma) mrna in activated human peripheral blood mononuclear cells (pbmncs) than nonpreincubation, indicating that vil-10 acts principally on monocytes. since the activation of monocytes and ...19921334711
epstein-barr virus infection and associated diseases in children. ii. diagnostic and therapeutic strategies.epstein-barr virus (ebv), an ubiquitous human b lymphotropic virus, is the cause of infectious mononucleosis. moreover, ebv infection can be followed by lymphoproliferative diseases in patients with inherited and acquired immunodeficiencies. primary ebv infection may be a threat to all children after marrow or organ transplantation or those receiving chronic immunosuppressive treatment for various other reasons. the virus has been also implicated in the pathogenesis of different malignant tumour ...19921334834
increased expression of epstein-barr virus in primary biliary cirrhosis patients.peripheral blood mononuclear cells (pbmc, n = 26), formalin-fixed paraffin-embedded liver tissues (n = 11) and saliva (n = 15) of primary biliary cirrhosis (pbc) patients were used for the detection of epstein-barr virus (ebv) sequences by polymerase chain reaction (pcr) assay. the semiquantitative analysis of ebv-dna was also carried out in a reconstructive experiment using an ebv-infected cell line. the pbmcs of pbc patients showed increased levels of ebv-dna (61%) in contrast to chronic activ ...19921334891
epstein-barr virus and interleukin-5 mrna in acquired immunodeficiency syndrome-related lymphomas with eosinophilia.patients infected with the human immunodeficiency virus are at increased risk for developing intermediate-grade and high-grade b-cell lymphomas that in many instances contain epstein-barr viral (ebv) dna. because interleukin-5 (il-5), a potent stimulator of eosinophil growth and differentiation, has been detected recently in ebv-infected b-cells, we hypothesized that some acquired immunodeficiency syndrome-related lymphomas with ebv dna also might contain eosinophilia and il-5. after reviewing f ...19921334944
extrachromosomal human immunodeficiency virus type 1 dna forms in fresh peripheral blood lymphocytes and in two interleukin-2-independent t cell lines derived from peripheral blood lymphocytes of an asymptomatic seropositive subject.two immature t cell lines (ft1 and ft4) were established after in vitro cloning of peripheral blood lymphocytes (pbls) from an asymptomatic human immunodeficiency virus type 1 (hiv-1) seropositive, human t cell-lymphotropic virus type 1 seronegative homosexual subject. although derived from a limiting dilution cell cloning assay, these cell lines were not recloned for this study. their growth was independent of exogenous interleukin-2. both cell lines were able to form colonies when cloned in ag ...19921335022
expression of genes for the epstein-barr virus small rnas eber-1 and eber-2 in daudi burkitt's lymphoma cells: effects of interferon treatment.the relative levels, rates of synthesis and stabilities of the abundant epstein-barr virus (ebv)-encoded small rnas, eber-1 and eber-2, were examined in daudi burkitt's lymphoma cells. although both rnas are transcribed at approximately equal rates, the steady-state level of eber-1 is at least 10-fold greater than that of eber-2. this is shown to be due to a much faster rate of turnover of eber-2. in the presence of actinomycin d, the half-lives of eber-1 and eber-2 are 8 to 9 h and 0.75 h, resp ...19921335024
high incidence of ebv genome in cd30-positive non-hodgkin's hodgkin's disease, epstein-barr virus (ebv) is found in cd30-positive reed-sternberg cells. we therefore studied 60 cd30-positive non-hodgkin's lymphomas (nhls) for the presence of ebv by the polymerase chain reaction (pcr) and dna in situ hybridization (dish), and by immunohistochemistry for the latent ebv proteins lmp and ebna-2. cd30-negative nhls and reactive lymph nodes served as controls. the cd30-positive cases comprised 17 anaplastic large cell lymphomas (alcls) (> 75 per cent cd30-po ...19921335046
[acute infections caused by epstein-barr virus. comparative study of various diagnostic methods].comparative performance of four methods (ifi, igm anti-vca, organon teknika; elisa, igm anti vca, incstar corp and pharmacia diagnostic inc; elisa igm against a group of several antigens which principal component is ebna, behring institute) to detect antibodies (abs) against epstein-barr virus in 180 patients (group 1) with infectious mononucleosis with no heterophil abs (organon teknika), 180 healthy subjects with no heterophil abs (group 2) and 20 patients with infectious mononucleosis (group ...19921335160
frequent oropharyngeal shedding of epstein-barr virus in homosexual men during early hiv determine the frequency of epstein-barr virus (ebv) oropharyngeal shedding during hiv infection in homosexual men in the multicenter aids cohort study.19921335273
anti-epstein-barr virus-nuclear antigen-1, -2a and -2b antibodies in rheumatoid arthritis patients and their relatives.we have examined serum antibodies to epstein-barr virus nuclear antigen (ebna)-1, -2a and -2b, in addition to antibodies to viral capsid antigen and early antigen in 100 rheumatoid arthritis patients and 50 of their relatives. using indirect immunofluorescence on transfected cells and western-blot technique, we have found increased frequency and titres of antibodies to ebna-2b in patients and, to a lesser degree, in their family members, whereas other anti-epstein-barr virus antibodies appeared ...19921335296
immortalisation of human antibody producing cells. 19921335297
clinicopathological spectrum of epstein-barr virus-associated t cell has been recently demonstrated that the epstein-barr virus (ebv) can infect human thymocytes and may be involved in the t cell neoplasms, in addition to african burkitt's lymphoma, nasopharyngeal carcinoma and hodgkin's disease. four distinct clinicopathologic categories of ebv-associated t cell malignancies have been recognized. the angiocentric t cell lymphoma or lymphomatoid granulomatosis involving the nose (or midline lethal granuloma) and skin is frequently ebv-associated. the other 3 g ...19921335323
[preparation of a monoclonal antibody against ea p138 of epstein-barr virus: its application in sjogren's syndrome].a monoclonal antibody against epstein-barr virus (ebv) encoded early antigen (ea) p138 was prepared with the use of a lysoprotein from the recombinant plasmid pucarg1140. by using this specific monoclonal antibody and immunofluorescence as well as immunoenzyme methods, 4 of 13 lacrimal, 15 of 37 labial and all 7 renal biopsy tissues from patients with sjogren's syndrome (ss) were found to have positive staining of epithelial cells, whereas those of control persons were all negative. these result ...19921335373
new heteromyeloma cell lines for the production of human monoclonal antibodies.the aim of this study was to establish hybridomas capable of long-term production of human monoclonal antibodies (mabs). heterohybridization was performed between the mouse myeloma cell line p3x63ag8.653 and activated human peripheral blood lymphocytes (pbl). in order to achieve better retention of human chromosomes, as well as to improve the stability of the heterohybrids, one hat-sensitive immunoglobulin (ig)-non-secreting human x mouse (h x m) heteromyeloma was fused for a second time with ac ...19921335463
activation of human herpesvirus-6 in children with acute measles.virological and serological studies were carried out prospectively to evaluate the possible activation of human herpesvirus-6 (hhv-6) in 50 infants and children with acute measles by isolation of hhv-6 from peripheral blood and by determining neutralizing antibodies to the virus. all but 5 patients (90%) were seropositive to hhv-6 in the acute stage of measles and 18 (40%) had a significant increase in hhv-6 antibody titers thereafter, whereas only 2 of 27 patients who were initially seropositiv ...19921335483
spectrum of mutations produced by specific types of restriction enzyme-induced double-strand breaks.rejoining of dna double-strand breaks (dsb) plays a central role in the various processes leading to dna rearrangements. we have analyzed dna alterations induced by restriction enzymes that produce dsb with specific types of ends. restriction enzymes were electroporated into a human lymphoblastoid cell line that stably maintains phaze, an ebv-based vector containing the lacz gene. after allowing time for dsb repair, phaze dna was rescued and screened in escherichia coli. mapping and sequence ana ...19921335541
correlative detection of human immunodeficiency virus (hiv) antigen p24 and epstein-barr virus dna in vitro: clinical influence on hiv infection.the existence of molecular transactivations between ebv and hiv-1, as well as reactivations of ebv latent infections in aids patients, have been recently documented. in order to shed more light on the putative association between ebv and hiv, and its role in the evolution to aids, we have determined simultaneously p24 protein and ebv dna in culture supernatants of peripheral blood mononuclear cells from 47 individuals suspected of having hiv infection. the results of the in vitro assays were cor ...19921335543
a prospective study of the polymerase chain reaction for detection of herpes simplex virus in cerebrospinal fluid submitted to the clinical virology laboratory.polymerase chain reaction (pcr) was prospectively performed with cerebrospinal fluid (csf) from 51 patients whose csf was available for analysis and was submitted for viral culture and/or herpes simplex virus (hsv) serology and 20 patients whose csf was submitted exclusively to the clinical biochemistry laboratory. primers were used that flanked a 92 bp segment of the hsv dna polymerase gene (35 cycles). amplified products were electrophoresed on agarose gel, blotted onto nylon membrane, and pro ...19921335547
transcriptional expression of the viral genome in the epstein-barr virus-induced tamarin lymphoma and the corresponding lymphoblastoid tumour lines.inoculation of the cottontop tamarin with epstein-barr virus (ebv) invariably gives rise to mono- or oligoclonal large cell lymphoma occurring at multiple sites, and which resembles to a certain extent b cell lymphoma that occurs in the immunodeficient patient. the viral transcriptional pattern in tamarin tumour biopsies and in the corresponding tumour cell lines was investigated by means of the synthesis of radioactive single-stranded cdna. it was found that the ebv transcripts came mainly from ...19921335674
protein kinase c regulates secretion of lymphotoxin (lt/tnf beta) and tnf alpha by human ebv-transformed b cells.epstein-barr virus (ebv)-transformed cell lines constitutively secrete lymphotoxin (lt/tnf beta) and not tumor necrosis factor-alpha (tnf alpha). to analyze the cellular processes that regulate lt and tnf alpha secretion by lymphoblastoid cell lines, we studied the role of two signal transduction pathways leading to either protein kinase c (pk-c) or pk-a activation. we demonstrate that pk-c activation, either after cross-linking of surface ig or by direct activation with phorbolester, leads to i ...19921335785
susceptibility of human monoclonal antibody-producing b cell lines to infection by human immunodeficiency immortalized b cell clones, five of which secreted specific human monoclonal antibodies (mabs) against hepatitis b, tetanus toxoid, and rhesus d antigens, were evaluated for their susceptibility to infection by human immunodeficiency virus types 1 and 2 (hiv-1 and hiv-2). infection was confirmed in three human mab-producing lines by detection of infectious virus and p24 antigen in culture supernates, by immunofluorescence, and by detection of viral dna in cells by polymerase chain reaction ...19921335786
manipulation of human b cells to confer immortality.electroporation was used to deliver genomic dna from a lymphoid tumor to activated/stimulated human peripheral blood lymphocytes to create immortalized lymphoid cell lines. activation of the recipient lymphocytes was essential for efficient immortalization. a panel of human b cell transfectant clones, each phenotypically representing specific stages of differentiation, resulted from the transfection. monoclonal antibody production was measured, and the level produced depended on the phenotype of ...19921335787
oligonucleotide fingerprinting as a means to identify and survey long-term cultured b cell hybridomas and t cell lines.common problems encountered during cell culture are cross-contamination, instability, and inadvertent exchange of cells. here we report on the application of oligonucleotide fingerprinting as a simple and efficient method to screen hybridomas and t cell lines. among the fingerprint probes tested, the simple repetitive oligonucleotide (cac)5/(gtg)5 proved to be most useful for obtaining many fragments specific for each cell line. because of variable loss of chromosomes, cloned hybridoma cells fro ...19921335788
epstein-barr virus, lymphomas and hodgkin's disease.epstein-barr virus (ebv) is implicated in the pathogenesis of a number of human lymphoid malignancies, including the immunoblastic b lymphomas that arise in immunocompromised individuals, burkitt's lymphoma, hodgkin's disease, and certain t cell lymphomas. the immunoblastic lymphomas are most likely a direct consequence of ebv-driven b cell lymphoproliferation that would in normal circumstances be eliminated by virus-specific cell-mediated immune responses. the other ebv-associated malignancies ...19921335791
epstein-barr virus and burkitt's lymphoma.recent investigations indicate that burkitt's lymphoma consists of several subtypes, defined by their clinical and molecular features. each geographical region so far studied appears to consist of a different mixture of subtypes. interestingly, there appear to be geographic 'gradients' with respect to the fraction of tumors associated with ebv and the type of 8;14 chromosomal translocation. the rate of ebv association is highest in equatorial africa, lowest in north america and intermediate in s ...19921335792
epstein-barr virus and nasopharyngeal carcinoma.nasopharyngeal carcinoma, an epithelial tumor which is characterized by marked geographic and population differences in incidence, is consistently associated with the epstein-barr virus (ebv). within the tumor, the ebv dna is homogeneous and clonal with regard to repeat sequences suggesting that the tumor is also clonal. expression of specific viral mrnas or gene products are consistently detected within all of the tumor cells. these data suggest that ebv is an essential component in the develop ...19921335793
epstein-barr virus-transformed b lymphocytes produce low molecular mass molecules with autocrine growth factor and competence factor activity.a human epstein-barr virus (ebv)-positive lymphoblastoid b cell line, named ba-d10-4, produces a factor of a molecular mass less than 10 kda that promotes cell proliferation of both ba-d10-4 cells and other human t or b lymphoid cell lines, either ebv-positive or -negative. the factor synergizes with higher molecular mass autocrine growth factors and makes both ba-d10-4 cells and b cell lines from burkitt's lymphoma, but not cells from t cell leukemia, more responsive to interleukin-1 and interl ...19921335948
[infections with herpesvirus 6--really only "exanthema subitum"? part 2: rare or unknown disease pictures].the human herpes virus 6 (hhv 6) may induce not only the wellknown condition of exanthem subitum, but also a number of more common (cf. part 1) or rare, even previously unknown, clinical manifestations. part 2 of this paper deals with the more rarely observed manifestations. these include complications of ard (sinusitis, otitis media, bronchial pneumonia) hepatitis, encephalitis or pfeiffer's disease (mononucleosis-like syndrome). in individuals with a relevant disposition (genetic hla/dr type?) ...19921335953
in situ hybridisation of ebv dna-dna hybrids using wet heat in polypropylene explore procedures designed to optimise dna-dna in situ hybridisation, using cells infected with epstein-barr virus (ebv) and tissues and subfragments of the ebv dna as probes.19921336018
[a study of epstein-barr virus-associated hemophagocytic syndrome successfully treated with vp16 and analysis of t cell receptor chain genes of the bone marrow cells].virus-associated hemophagocytic syndrome (vahs) is a nonneoplastic, generalized histiocytic proliferation disorder with marked hemophagocytosis associated with a systemic viral infection. we describe a female child with ebv-related vahs, in whom southern blot analysis showed monoclonal proliferation of bone marrow cells having ebv genome as detected with xho-1 fragment of latent infection membrane protein (lmp) genome. the ebv serology showed anti-ebna, anti-vca-igg, anti-vca-iga elevation and p ...19921336076
long synthetic oligonucleotide probes for gene analysis. 19921336090
fidelity of replication of the leading and the lagging dna strands opposite n-methyl-n-nitrosourea-induced dna damage in human cells.semi-conservative replication of double-stranded dna in eukaryotic cells is an asymmetric process involving leading and lagging strand synthesis and different dna polymerases. we report a study to analyze the effect of these asymmetries when the replication machinery encounters alkylation-induced dna adducts. the model system is an ebv-derived shuttle vector which replicates in synchrony with the host human cells and carries as marker gene the bacterial gpt gene. a preferential distribution of n ...19921336179
in situ demonstration of epstein-barr virus in intravenous drug abusers with generalized lymphadenopathy.we have studied by the in situ hybridization method the presence of epstein-barr virus (ebv) dna genome in lymph node tissues from 11 patients with persistent generalized lymphadenopathy. using a biotinylated ebv dna probe, we demonstrated ebv nucleic acid in scattered germinal centre cells in eight of the 11 cases. our results suggest that ebv is not a determinant factor in the pathogenesis of this lymphadenopathy, but support its possible implication in b cell malignant transformation in cases ...19921336194
molecular biologic studies of hodgkin's disease.there has been intense interest in applying the techniques of molecular biology to further our understanding of hodgkin's disease. numerous studies of the status of lymphocyte antigen receptor genes have been performed, with conflicting results. there is more uniformity in the results of epstein-barr viral (ebv) studies; evidence of ebv can be identified in the reed-sternberg cells of approximately 40% to 50% of cases. there is no consensus on whether the t(14;18) is present in tissues involved ...19921336209
molecular biology of bovine herpesvirus type 4.bovine herpesvirus type 4 (bhv-4) is a ubiquitous virus of cattle. its genome is a 144 +/- 6 kb double-stranded dna consisting of a unique central part (l-dna) flanked at both ends by tandem repeats called polyrepetitive dna (prdna or h-dna). the overall arrangement of genes has been obtained by the analysis of homologies between short bhv-4 dna sequences and corresponding genes of epstein-barr virus (ebv) and herpesvirus saimiri (hvs). the gene expression is temporally regulated. glycoprotein p ...19921336253
repression of the viral latent promoter bc-r2 in epstein-barr virus negative cell lines.the regulation of the latent promoter bc-r2 of epstein-barr virus (ebv) was examined using the chloramphenicol acetyl transferase (cat) gene reporter system. a 5' deletion analysis of bc-r2 promoter sequences has been used to characterize a region, described previously as a transcriptional enhancer in ebv positive cell lines, which can repress the bc-r2 transcriptional activity in ebv negative cell lines.19921336378
ebv infection patterns in hodgkin's disease and normal lymphoid tissue: expression and cellular localization of ebv gene products.the present study was performed to clarify the reported inconsistencies regarding the frequency of the association of epstein-barr virus (ebv) and hodgkin's disease (hd). biopsies from 102 patients with hd were screened for the presence of ebv-encoded small nuclear rna (eber) and latent membrane protein (lmp) by using a non-isotopic in situ hybridization (ish) and immunohistology (ih), respectively. the results were additionally compared with those obtained by polymerase chain reaction (pcr) for ...19921336392
detection of epstein-barr viral dna in aggressive cd8+ t cell leukaemic cells. 19921336393
functional characterization of human il-10.rapid progress has been made over the past two years in the characterization of the biological activities of interleukin-10. interleukin-10, produced by t cells, b cells, macrophages/monocytes and keratinocytes, alters profoundly the morphology, the expression of mhc class ii antigens and the production of cytokines by monocytes which in turn can affect a variety of immunological responses including antigen specific proliferation and cytokine production of both soluble and allo-antigens by t cel ...19921336421
detection of mutations in the p53 gene in human head and neck carcinomas by single strand conformation polymorphism analysis.using the polymerase chain reaction (pcr)-based single strand conformation polymorphism (sscp) analysis, we have examined the highly conserved regions of the p53 gene in 58 biopsy samples of head and neck tumors. mutations were found in 13/58 (23%) tumor specimens, but not in 6 normal tissues. ten of 13 mutations were due to single base changes and the remaining 3 were 1- or 8-base deletion mutants. these mutations were clustered in exons 5 and 7 and resulted in amino acid changes. our results s ...19921336431
fallacies in the interpretation of paul-bunnel davidsohn differential test.serological findings in five cases where paul-bunnel davidsohn (pbd) test results were misleading, are presented. three patients' with chronic renal disorder and positive pbd test had specific serology results, signifying cyto megalo virus infection. a fourth patient with hepatitis b virus infection also had positive pbd test. forssman type of antibody response was demonstrable in a boy with recent epstein-barr virus infection and high cyto megalo virus antibody titres.19921336481
epstein-barr virus (ebv) and hodgkin's disease in children: incidence of ebv latent membrane protein in malignant cells.previous studies have detected ebv dna by southern blotting or in situ hybridization in biopsy material from up to 30 per cent of adult cases of hodgkin's disease. here we have used monoclonal antibodies specific for the ebv latent membrane protein lmp1 to examine archival material from children with hodgkin's disease. material from 74 cases (54 males and 20 females) was examined and 37 (30 males and 7 females) were classified as lmp1-positive in the malignant cells. lmp1 positivity was present ...19921336543
[the ebna2 coding region in the viral genome of epstein-barr virus (ebv)-positive cases of hodgkin's disease].epstein-barr virus (ebv)-genome containing cases of hodgkin's disease (hd) are known to express latent membrane protein (lmp) of ebv but no ebv nuclear antigen 2 (ebna2). we investigated 35 cases of hd for the presence of ebv genome sequences to know whether ebna2 coding region is deleted in hd. 27 cases had general ebv sequences, none had evidence of a deletion of ebna2. 26 cases turned out to harbour ebv type 1, one case had ebv type 2. the absence of ebna2 protein expression in hd can not be ...19921336913
sjögren's syndrome: review with recent insights into immunopathogenesis. 19921336955
expression of b-type epstein-barr virus in hiv-infected patients and cardiac transplant the present study, peripheral blood mononuclear cells (pbmcs) were obtained from hiv+ subjects as well as cardiac transplant recipients, and the presence of a- and b-type epstein-barr virus (ebv) was determined using the polymerase chain reaction (pcr). of the hiv+ patients studied, 24% were found to be infected with a-type ebv, 27% with b-type ebv, and 39% with both a and b virus types. analysis of pbmcs from cardiac transplant recipients revealed that 39% were infected with a-type ebv, 33% ...19921336962
recombinant human growth hormone promotes human lymphocyte engraftment in immunodeficient mice and results in an increased incidence of human epstein barr virus-induced b-cell lymphoma.recombinant human growth hormone (rhgh) previously has been demonstrated to promote human or mouse t-cell engraftment in immunodeficient mice. we then wanted to examine long-term effects of rhgh on human cell engraftment in these mice. mice with severe combined immune deficiency (scid) were given human peripheral blood lymphocytes or human bone marrow cells and daily injections of rhgh (20 micrograms ip every other day). upon later assessment for engraftment by flow cytometric analysis, it was d ...19921336993
mutational analysis of the transforming function of the ebv encoded lmp-1. 19921337030
integration of ebv in burkitt's lymphoma cells. 19921337031
the oncogenic potential of epstein-barr virus nuclear antigen 1 in transgenic mice. 19921337032
aids associated non-hodgkin's lymphomas represent a broad spectrum of monoclonal and polyclonal lymphoproliferative processes. 19921337033
large granular lymphocytosis terminating in a polymorphous b-lymphocytic proliferation after low-dose cyclophosphamide therapy: a case report with necropsy findings.a 70-year-old man presented with clonal large granular lymphocytosis of t-suppressor/cytotoxic immunophenotype, neutropenia, paraproteinemia, and proneness to infection. the patient became severely leukopenic during 14 days of chemotherapy with low-dose cyclophosphamide, and remained so after discontinuation of the drug. clinically, he was thought to have prolonged chemotherapy-induced marrow hypoplasia. at death, 16 days after the last dose of chemotherapy, autopsy confirmed bone marrow hypopla ...19921337079
antigenic expression of b-cell chronic lymphocytic leukemic cell lines.a flow cytometric analysis of five b cell chronic lymphocytic leukemic (b-cll) cell lines was undertaken using 129 unknown reagents from the blind panel (bp) and 72 reagents from the known cd panel obtained from the fourth international leucocyte differentiation conference and workshop, b cell section (vienna, 1989). the five cell lines examined were: sed (pnas 75, 5706, 1978), b-cll-lcl (blood 71, 9, 1988), jvm-hh and jvm-2(int j can 38, 531, 1986), and wr#1 (th and bd). the reagents were #1-12 ...19921337293
effects of phorbol esters and cytokines (interleukin-2,-4, and -6) on the proliferation and surface phenotype of epstein-barr virus immortalised human b lymphocytes.epstein-barr virus (ebv)-induced in vitro infection of peripheral blood mononuclear cells (pbmcs) leads to a polyclonal proliferation and immortalisation of b lymphocytes. in the present study we determined the effects of three different cytokines, interleukin-2 (il-2), interleukin-4 (il-4) and interleukin-6 (il-6), and the tumour promoting phorbol ester 12-o-tetradecanoyl-phorbol-13-acetate (tpa) on ebv-immortalised b lymphocytes. these factors have known activities on normal b cells. il-4 and ...19921337296
[study by in situ hybridization of the prevalence of herpes virus as cofactors of the tumoral development of papillomatous lesions of the anogenital region in seropositive and seronegative men against hiv].infection with specific types of hpv has emerged as necessary but not sufficient factor in the neoplastic transformation of anogenital condylomas. some viruses (hiv, herpes viridae: hsv, cmv, ebv) might act as cofactors in the neoplastic changes and cancer. to study the prevalence of these viral pathogens in anogenital lesions, biopsies were obtained from hiv seropositive or seronegative men and tested using in situ hybridization technique. infection by "high risk" hpv, hsv and cmv are facilitat ...19921337305
accumulation of p53 protein correlates with tumour proliferative activity in ebv positive burkitt's lymphoma.p53 tumour suppressor gene is often found mutated in burkitt's lymphoma (bl) cell lines and tumours. we analysed 35 bl tumours for the accumulation of p53 protein, and correlated the results with dna flow cytometric data on the proliferative activity (spf), and data on the presence or absence of epstein-barr virus (ebv) dna. more than one-third (37 per cent) of the tumours showed accumulation of p53, which was considered to be consistent with mutation of the p53 gene. tumours that were positive ...19921337333
chronic active epstein-barr virus infection in an adult.we report a rare adult case of chronic active epstein-barr virus (ebv) infection. a 54-year-old woman was admitted to our hospital with intermittent fever, weight loss, hepatosplenomegaly, pancytopenia and liver disturbance. in serological tests for ebv, anti-virus capsid antigen (vca)-igg antibody and anti-early antigen (ea)-igg antibody were markedly elevated and anti-ebv nuclear antigen (ebna) antibody was negative. ebv genome was detected in the bone marrow nucleated cells and peripheral lym ...19921337484
prevalence of herpesvirus, human t-lymphotropic virus type 1, and treponemal infections in southeast asian refugees.sera obtained for treponemal serology (vdrl) from 193 southeast asian refugees representing five ethnic groups seen in a primary care clinic were examined for antibodies to human t-lymphotropic virus type 1 (htlv-1), human herpes-virus-6 (hhv-6), epstein-barr virus (ebv), and cytomegalovirus (cmv). the seroprevalence was highest for ebv (99%), followed in decreasing order by cmv (95%), hhv-6 (26%), and htlv-1 (0.6%). the vdrl was positive in 15% of patients. the highest seroprevalence to hhv-6 w ...19921337548
use of antigen expressed in bacteria for detection of ebv-specific thymidine kinase antibodies in sera from patients with nasopharyngeal carcinoma.two cdna clones covering the n- and c-terminal portions of the ebv bxlf1 open reading frame were selected from a cdna library derived from p3hr1 cells. the two clones were ligated, the n-terminal untranslated region truncated, and the product inserted into an e. coli expression vector, pet3cp*. the fusion protein was expressed under control of the t7 phage phi 10 gene promoter and shown to possess thymidine kinase activity. the protein was then used as an antigen to detect antibody reactivities ...19921337549
[chronic fatigue syndrome and virus infection: human herpesvirus 6 (hhv-6) infection].chronic fatigue syndrome (cfs) is newly-recognized disease characterized by chronic and debilitating fatigue. it has been suggested that viral infection may be involved in this syndrome from the results of clinical examination, including increased activity of 2',5'-synthetase in leukocytes of patients. the following viruses have been reported as etiologic agents of this disease. first, many studies have found elevated levels of igg to viral capsid antigen and early antigens to epstein-barr virus ...19921337558
[viral infection and its causative role for chronic fatigue syndrome].patients with chronic fatigue syndrome (cfs), of unknown etiology, have been increasingly reported. this syndrome is characterized by debilitating fatigue, lymphadenopathy, and fever. herein, i focus on and review this syndrome from the view point of the causative role of viral infection. since the symptoms of cfs are similar to those of chronic infectious mononucleosis (cim) or chronic epstein-barr virus infection (cebv), the role of ebv has been intensively studied. the etiological relationshi ...19921337559
[chronic fatigue syndrome--study of 51 cases treated at the second tokyo national hospital].fifty-one patients with chronic fatigue syndrome (cfs) were studied. tender points, which are a characteristic clinical feature of fibromyalgia, were found in all but two of the patients at 11.4 points (mean) per patient. igg antibody titers to eb virus viral capsid antigen were more elevated in the cfs patient group compared to that of the control (p < 0.0015). igg antibody titers to hhv-6 were not higher in the patient group. nk cell activity was not more decreased in the patient group, wherea ...19921337560
[chronic fatigue syndrome--cases in the kanebo memorial hospital].in our hospital, 134 patients (28 male, 106 female, 10-82 years of age) were diagnosed as having chronic fatigue syndrome (cfs). some patients had mild elevation of antibodies against epstein-barr virus and immunologic abnormalities (natural killer cell dysfunction and high rates of skin reactivity to house dust, pollen, drugs and common food). in the patients with immunologic abnormalities, we found decreases in serum concentrations of arachidonic acid and dihomogamma-linolenic acid. a kampo me ...19921337561
[symptoms, signs and laboratory findings in patients with chronic fatigue syndrome].this review summarizes the symptoms, signs and laboratory abnormalities seen in 59 patients with chronic fatigue syndrome (cfs), 2 patients with post-infectious cfs and in 26 patients with possible cfs whose illnesses fulfill the criteria proposed by the study group of the ministry of welfare, japan. the characteristic symptoms and signs of cfs are prolonged generalized fatigue following exercise, headache, neuropsychological symptoms, sleep disturbance and mild fever. in possible cfs patients, ...19921337562
[a virus from the herpes group and pseudothrombocytopenia: two cases]. 19921337622
is persistent polyclonal b lymphocytosis caused by epstein-barr virus? a study with polymerase chain reaction and in situ hybridization.previous reports of patients with persistent polyclonal b lymphocytosis have found associations with female sex, cigarette smoking, hla-dr7 phenotype, and moderate elevation of peripheral blood polyclonal b lymphocytes. the presence of binucleated cells and atypical lymphocytes in the peripheral blood of these patients was highly suggestive of a viral infection, such as with the epstein-barr virus. we report a 47-year-old asymptomatic woman who was incidentally found to have persistent polyclona ...19921337664
[variants of the interaction of the human t-cell leukemia virus (htlv-i) with the b-cells of lymphoblastoid lines].b-cell lymphoblastoid lines which are known to be derived by in vitro inoculation of b-lymphocytes with epstein-barr herpes virus (ebv) were shown to be infected with htlv-1. three possible variants of htlv-1 interaction with cells were demonstrated by immunoblot, polymerase chain reaction, and virus isolation: (1) prolonged productive infection; (2) infection of the cells manifested only by the presence of "silent" virus sequences; (3) temporary production of htlv-1 (3.5 months) after the end o ...19921337802
[human hybridoma neutralizing antibodies to the herpes simplex virus].human hybridoma cells producing monoclonal antibodies to human herpes simplex virus were generated. the antibody produced by the hybrid cells interacted with herpes virus types i and ii in enzyme immunoassay and complement fixation test, had neutralizing activity both in vivo and in vitro. these human monoclonal antibodies may be used for immunotherapy of herpes virus infection.19921337804
recent results on the biology of hodgkin and reed-sternberg cells. i. biopsy material.the most recent sophisticated investigations have provided new and revealing, but also contradictory and controversial information on the biological nature and the cellular origin of hodgkin and reed-sternberg cells (h-rs). immunophenotypic analyses have shown variable phenotypic antigen expression; but, on balance the data suggest a lymphoid cell expressing t- and/or b-cell-associated markers and certain activation antigens while lacking immunological features of monocytes-macrophages or other ...19921337848
significance of the detection of epstein-barr virus dna in lymph nodes in patients with hodgkin's disease.epstein-barr virus (ebv) dna is frequently identified in benign and malignant lymphoproliferative conditions. as shown by in situ hybridization studies viral dna is localized within malignant cells as well as benign lymphocytes. clonal and nonclonal ebv genomes are present in hodgkin's disease (hd), lymphomas of the immunocompromised host and reactive lymph node hyperplasia. lytic infection with formation of linear genomes is observed in the same conditions but appears to be infrequent in hd as ...19921337849
detection of cellular ki-ras oncogene and epstein-barr viral dna by polymerase chain reaction in situ.a newly established technique of polymerase chain reaction (pcr) in situ is reported. the process involved the use of the pcr technique to amplify the target gene and to generate the radiolabelled products which was used as a probe to hybridize the target gene in situ. ki-ras oncogene in human pancreatic carcinoma cell line (pc-2) and ebv dna in raji cell line were detected in situ by this technique with encouraging results. this technique has the advantages of both pcr and in situ hybridization ...19921337873
is multiple sclerosis caused by a dual infection with retrovirus and epstein-barr virus?although the etiology of multiple sclerosis is as yet unknown, epidemiological observations strongly point toward one or more infectious agent(s) being involved in the disease. in recent years some studies have indicated involvement of retrovirus in multiple sclerosis (ms). however, an intrafamilial epidemiological study revealed that ms and the known human retroviruses had a divergent epidemiology. some studies have shown the association of epstein-barr virus (ebv) with ms and one recent study ...19921337948
[simultaneous detection by non-isotopic in situ hybridization of human papilloma viruses and epstein-barr virus during the lytic cycle in oral hairy leukoplakia lesions].oral hairy leukoplakia is almost only described in patients infected by the human immunodeficiency virus. epstein-barr virus, sometimes associated with human papillomavirus, is always involved in the occurrence of these lesions. we have investigated two cases of oral hairy leukoplakia with the goal of detecting ebv and hpv by using both in situ hybridization and immunohistochemistry. ebv genome was detected with biotinylated bamhi w cdna probe in the two cases. furthermore, ebv was found to be i ...19921338183
[lymphoproliferative syndromes associated with epstein-barr virus in transplantation].we report 23 cases of lymphoproliferative diseases which occurred among 2,100 patients with kidney or combined kidney+pancreas transplant. eleven patients developed a severe diffuse disease within the first 3 months post-transplantation; immunoblastic b cells of recipient origin infiltrated the bone-marrow, transplanted organs, liver, spleen, lymph nodes, lungs, and brain; immunoglobulin abnormalities with fever, leuko-thrombocytopenia and liver dysfunction constituted the symptoms; all patients ...19921338226
[nasopharyngeal carcinoma and hereditary angioneurotic edema]. 19921338398
an adult case of severe chronic active epstein-barr virus infection syndrome.a 23-year-old man with persisting high fever developed hepatosplenomegaly, lymphadenopathy and massive pericardial effusion. immunological examination revealed a marked elevation of anti-epstein-barr virus antibodies (anti-viral capsid antigens igg-antibody 1:10,240, anti-early antigens-dr igg-antibody 1:5,120), decreased activities of epstein-barr virus specific cytotoxic t lymphocytes, natural killer cells and lymphokine activated killer cells. a liver biopsy showed moderate sinusoidal lymphoc ...19921338620
transformation of human colostrum lymphocytes with epstein-barr virus.lymphocytes from human colostrum were transformed with eb virus to obtain immunoglobulin secreting cells. equal proportions of igg, iga and igm were detected in wells containing transformed colostrum cells. pretreatment of colostrum cells with bcgf prior to eb virus infection reduced the transformation slightly. we discussed the possibility of establishing cell lines that produced human monoclonal antibody using colostrum cells.19921338659
the cytokines effect on ebv-immortalized human b cells producing antisperm antibody.we have analyzed the antisperm antibody production of autoimmunized male subjects using epstein-barr virus (ebv) immortalization of b lymphocytes. we evaluated the influence of several in vitro culture variants applied prior to ebv infection on the frequence of antibody-producing cells and affinity of secreted antibodies. the following variants were applied: a) polyclonal antigenic stimulation of lymphocytes with pwm, b) pwm (pokeweed mitogen) + il-2 + interferon gamma and c) pwm + il-2 + interf ...19921338684
cd43 is expressed normally on wiskott-aldrich-derived lymphocytes.the wiskott-aldrich syndrome (was) is an x-linked disease characterized by eczema, thrombocytopenia, and profound immunodeficiency in affected males. while the etiology of the syndrome is currently unknown, abnormalities of cd43 have been described as a biochemical marker of the disease. several investigators have demonstrated alterations in the expression of the cd43 surface antigen on was hematopoietic cells, noting either absence, decreased levels or changes in the characteristic molecular we ...19921338889
evidence for abnormally regulated alternative rna processing of mu chain gene in b-lymphoblastoid cells from bloom's syndrome.selective igm deficiency is commonly found in patients with bloom's syndrome. in this study, mu mrna synthesis was investigated in b-lymphoblastoid cells transformed by epstein-barr virus (lcl) from a patient with bloom's syndrome who showed selective igm deficiency. lcl established from the patient with bloom's syndrome well expressed igm molecules in their surface, but scarcely produced secreted igm, compared with healthy controls. the jh hybridization patterns of digested dna of lcl from the ...19921338890
[construction of an eb virus based shuttle vector plasmid pzh32].the shuttle vector can replicate in both bacteria and eukaryotic cells. we have constructed a shuttle vector pzh32 based on the eb virus. three component parts of the plasmid come from psv2gpt, ps189 and pmci5. an e. coli tyrosine suppressor trna gene, supf, was used as a mutagenic target in this plasmid, and a gpt gene was used as a selectable marker gene for transformed cells. this pzh32 based on ebv replicates autonomously in the nuclei of human cells. it has a low background mutation frequen ...19921338950
historical progress and the future of human cell culture research.progress in human cell culture research is discussed based primarily on our hematopoietic cell culture studies. the article includes a historical background of burkitt lymphoma cell lines, discovery of ebv, normal b-lymphoblastoid cell lines with ebv, a variety of leukemia, lymphoma, and myeloma cell lines, clinical and theoretical contributions made by studies of t-cell leukemia cell lines, the discovery and clinical relevance of htlv, hiv and hblv, early attempts at adoptive immunotherapy of p ...19921339005
wild long-tailed macaques (macaca fascicularis) in thailand are highly infected with gamma herpes virus but not with simian t-lymphotropic retrovirus of type 1. 19921339088
three monoclonal antibodies against human lfa-1 alpha and beta chains with different biological activities.three murine monoclonal antibodies (mabs) directed against human lymphocyte function-associated antigen-1 (lfa-1, cd11a/cd18), designated as may.017, may.035, and may. 044, were newly generated. the hybridomas were screened for their ability to inhibit a phorbol ester-stimulated aggregation of epstein-barr virus-transformed b-lymphoblastoid cell line (b-lcl). the mabs bound to peripheral blood leukocytes, t cell lines, b cell lines, and some of myeloid/monocytic lines, but not to b-lcl derived f ...19921339124
[epidemiological analysis of infectious mononucleosis as primary epstein-barr virus infection].we report the characteristics of 45 epstein-barr virus (ebv)-infected patients visiting our hospital from 1989 to 1991 in comparison with 102 patients seen from 1981 to 1988. cases of infectious mononucleosis (im) increased especially in patients over 30 years old. they tended to have nonspecific symptoms and signs for im such as general fatigue. immunologically showed twice higher positivity for anti-ebv vca-igm antibody in the recent 3 years when compared to the previous 8 years. the patients ...19921339163
prevalence of antibody to epstein-barr virus dnase in children.antibodies to epstein-barr-virus-specific dnase in children under 15 years old from taipei, taiwan were determined by an enzyme neutralization test. serum was taken as positive for the anti-dnase antibody when 1 ml of serum could neutralize > or = 2 units of the dnase activity. among the 1,292 sera tested, 5.6% of the children were shown to have anti-ebv dnase antibodies, a result similar to that for adults. maternal antibodies against the ebv dnase were found to decrease very rapidly 2 months a ...19921339186
comparative sequence analyses of epstein-barr virus nuclear antigen-2 and -3b genes of a fresh epstein-barr virus-2 isolate.the nucleotide sequence of epstein-barr virus (ebv) nuclear antigen (ebna)-2 and ebna-3b regions of an ebv-2 isolate in japan was analyzed. the deduced amino acid sequences of ebna-2 and ebna-3b of the isolate and a prototype ebv-2 strain ag876 were identical, but the number of the repeated sequence in ebna-3b was variable as that of ir-3 in ebna-1. the remarkably well-conserved sequence seems to suggest that the structure of the ebna-2 and ebna-3b genes of ebv-2 is crucial for their function in ...19921339417
epstein-barr viral latency and cell immortalization as targets for antisense oligomers.the approach of using an antisense oligonucleotide to oppose the synthesis of a single selected viral gene product needed to maintain the ebv episome in non-virus-producing cells appears to be promising not only for possible cure of latent viral infection, but also for reversal of ebv-driven cell proliferation. it is also possible that targeting multiple latent viral genes might mount a synergistic effect that would prove to be more efficient at curing latent infection. however, further work is ...19921340114
effects of cyclosporine on human b-cell lymphoma development in vivo.cyclosporine (csa) is a potent immunosuppressive agent primarily affecting t-lymphocyte function. patients receive csa following organ transplantation to prevent rejection. these patients are at high risk for developing epstein-barr virus (ebv)-induced lymphoproliferative disease (lpd) or b-cell lymphoma (bcl). severe combined immunodeficient (scid) mice reconstituted with human peripheral blood leukocytes (pbl) develop fatal b-cell lymphomas of human origin following latent or active infection ...19921341239
possible viral implication in the pathogenesis of sjögren's then could the observed pathological changes in the exocrine glands of patients with pss be related to the various in vitro and in vivo laboratory findings? to begin with, the fact of inappropriate hla-d/dr expression in epithelial cells in the absence of any infiltrating t cells or residual ifn-gamma must argue for an exogenous agent such as a virus that modulates the gene expression of epithelial cells. such inappropriate expression is probably accompanied by the expression of other molecu ...19921341448
primary central nervous system lymphoma.primary central nervous system lymphomas (pcnsl) are uncommon neoplasms accounting for less than 2% of brain tumours. their incidence appears to be increasing across a wide age range, in both immunocompetent and immunosuppressed populations. particular risk groups include those with congenital and acquired immunodeficiencies and transplant recipients. the spread of the aids epidemic has seen large numbers of complicating pcnsl develop. epstein-barr virus infection appears to play a role in the d ...19921341961
epstein-barr virus and carcinomas. expression of the viral genome in an undifferentiated gastric carcinoma.the epstein-barr virus (ebv) is associated with undifferentiated nasopharyngeal carcinomas (npcs). recently, ebv dna has been demonstrated also in some cases of gastric carcinoma with similar morphology as undifferentiated npc. here we report on the expression of ebv genes in a gastric undifferentiated carcinoma of nasopharyngeal type (ucnt). the small ebv-encoded nuclear rnas, ebers, were expressed in all tumor cells. the bzlf1 transactivator protein, which disrupts viral latency, was detectabl ...19921342957
description of an in situ hybridization methodology for detection of epstein-barr virus rna in paraffin-embedded tissues, with a survey of normal and neoplastic tissues.the authors describe a highly sensitive and practical in situ hybridization method using an oligonucleotide probe for eber1 rna for the detection of epstein-barr virus (ebv) in formalin-fixed, paraffin-embedded tissue sections. paraffin-embedded tissues from 793 cases of normal and neoplastic tissues were studied. nuclear staining for ebv rna was uniformly present in all or virtually all neoplastic cells in a variety of known ebv-positive tumors. we also demonstrate rare ebv-infected cells in no ...19921342973
three cases of primary cerebral lymphoma in aids patients: detection of epstein-barr virus by in situ hybridization and southern blot technique.three cases of primary cerebral lymphoma in acquired immunodeficiency syndrome were studied. tumoral fragments taken at autopsy were frozen and studied by the southern blot technique (sbt). other tumoral fragments were fixed in formalin, embedded in paraffin and used for in situ hybridization (ish) with biotinylated probes for dna of epstein-barr virus (ebv). ish was positive in each case with a spotty nuclear labelling of certain tumoral cells. sbt evidenced a clonal rearrangement of the immuno ...19921343083
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