superinfection of ebv-carrying lines with herpes virus papio (hvp): induction of early antigen (ea) and inhibition of cellular dna synthesis. 1979220052
cell surface glycoprotein changes in epstein-barr virus-positive and -negative human hematopoietic cell has previously been shown that differential fucose labelling of many normal and homologous tumor cells, followed by proteolytic release and degradation, yields glycopeptides which upon gel filtration shown an increase in fast-eluting glycopeptides for the tumor cells. this technique has now been applied to cell-surface glycoproteins of different human hematopoietic cell lines. these lines included epstein-barr virus (ebv)-carrying lymphoblastoid cell lines of presumed non-neoplastic origin, a ...1979220199
establishment and characterization of a human null-cell lymphoblastic lymphoma cell line (k-ll-3).a new cell line (k-ll-3) with exceptional characteristics was derived from a bone-marrow aspirate from a patient with poorly differentiated lymphoblastic lymphoma. the cell line was established and the cells maintained on agar feeders for over 1 year. the morphology and cytochemical staining of the primary tumor and the cell line were remarkably similar. the growth characteristics, chromosome pattern, cell-surface receptors and epstein-barr pattern, cell-surface receptors and epstein-barr virus ...1979220200
distribution of t- and b-cells and of immunoglobulin-producing cells in tumor tissue of patients with nasopharyngeal carcinoma.various subtypes of 17 european nasopharyngeal carcinomas are examined by immunocytological and immunohistological methods. the t- and b-cells in the inflammatory infiltrate are determined quantitatively and correlated with the amount and type of intracellular immunoglobulin. this data is compared with peripheral t-cell values and the serum anti epstein-barr virus (ebv) titers, and finally with the tumor type and stage. three of the 17 patients had marked local immunoglobulin producing cells, 8 ...1979220268
primary infection with epstein-barr virus in infants in the united states: clinical and serologic observations.previous studies in ghana had shown that primary infections with epstein-barr virus in infants under the age of two years remain silent and evoke antibody responses different from those seen in infectious mononucleosis. in order to determine whether or not these observations were limited to africa, 80 american infants presenting with minor infectious complaints were studied serologically; 14 (17.5%) showed evidence of recent or current primary infections with epstein-barr virus. the clinical fea ...1979220340
[search for unknown viruses of human neoplasms]. 1979220344
[experimental studies on viral etiology of epstein-barr (ebv) in nasopharyngeal carcinoma (npc):establishment of ebv infection on human epithelial derived from nasopharyngeal tissue (author's transl)]. 1979220401
nasopharyngeal carcinoma in an alaskan eskimo family: report of three cases.nasopharyngeal carcinoma (npc) has been reported to occur in alsaka natives (eskimos, indians, and aleuts) at a rate greater than 15 times the rate for u.s. whites. at least 3 cases of npc have occurred in close relatives of a northern alaskan eskimo family. all 3 patients had poorly differentiated tumors and have died. blood groups, hla antigens, and antibodies to epstein-barr virus (ebv) were studied in the family members. ebv titers were elevated in the 1 npc patient tested and in 1 of 15 oth ...1979220446
extraction of soluble antigens of epstein-barr virus, herpesvirus salmirl, and herpesvirus ateles with the use of glycine.for extraction of soluble antigen from cells infected with epstein-barr virus, herpesvirus salmirl, and h. ateles, 0.1 m glycine (ph 9.5) was used. this method yielded increased amounts of the antigen containing much less cell debris. lymphoblastoid cells infected with epstein-barr virus could maintain up to 50% viability after the extraction procedure. these cells could be used again after an appropriate interval in culture. the usefulness of this technique is discussed.1979220447
[epstein-barr virus. its participation in lymphoproliferative and anaplastic processes]. 1978220495
prolonged survival in chronic lymphocytic leukemia: a case report.a patient with long-standing chronic lymphocytic leukemia with both humoral and cellular immunodeficiency had lymph node receptor evidence of a b lymphocyte disorder. he was also found to secrete the epstein--barr virus and, late in his illness, developed a markedly positive antinuclear antibody. interrelationship of these findings may be important in the ultimate determination of the etiology and functional mechanisms in lymphocyte malignancies.1979220516
induction of the epstein-barr virus (ebv) cycle in latently infected cells by n-butyrate. 1979220786
a possible function of epstein-barr virus-determined nuclear antigen (ebna): stimulation of chromatin template activity in vitro. 1979220793
the virology and immunology of burkitt's lymphoma in africa and the need for corresponding studies in the high endemic regions of papua new guinea. 1978220813
cancer biology. iii. herpes virus epidemiology, molecular events, oncogenicity and therapy. 1976220850
in vitro transformation of mammalian cells by human herpes viruses. 1976220851
the epstein-barr virus and human malignancies. 1976220855
some aspects of the biologic behavior of epstein-barr virus. 1976220856
the sero-epidemiology of epstein-barr virus. 1976220858
[antiviral activity of interferon and its inducers in human lymphoblastoid and somatic cells].the antiviral effect of interferon inductors, such as poly-i--poly-c, phage f2 rna replicative form and low molecular inductor gsn and their influence on cellular dna synthesis were studied in the cultures of lymphoblastoid (inplanting lines raji namalva) and somatic human cells. the semliki forest virus used as the test organism multiplicated well in cells raji accumulating up to 9 lg bou/ml. the two-strand rna was less active in the lymphoid cells than in the somatic ones. gsn was 10 times mor ...1979220908
retrovirus-like particles in ebv-negative burkitt's lymphoma cell line but not in ebv-dna-positive lines from patients with ataxia telangiectasia and down's syndrome.retrovirus-like particles can be recovered by arginine deprivation from the bjab-1 epstein-barr virus (ebv) negative cell line derived from an african patient with typical burkitt's lymphoma. these particles resemble retroviruses in their morphology and in their physicochemical properties. particles with a similar morphology were obtained from derivative cell lines established by infecting bjab-1 cells with ebv. on the other hand, retrovirus-like particles could not be induced in ebv-dna-positiv ...1979220999
chromosome aberrations in b lymphocytes of atomic bomb survivors.although chromosome aberrations in t lymphocytes and bone marrow cells have been reported in atomic bomb survivors, the presence of chromosome abnormalities has not been demonstrated in b lymphocytes because of the technical difficulties involved in b-lymphocyte separation. a method for detecting chromosome aberrations in b lymphocytes was established by "stimulation" of b lymphocytes with epstein-barr virus (ebv) instead of "separation" of b lymphocytes by rosette formation. the ebv-stimulated ...1979221056
in vitro growth of cutaneous t-cell is relatively easy to propagate eb virus-transformed normal and malignant b lymphocytes in vitro. normal t lymphocytes divide for short periods of time after exposure to many mitogens. exposure of normal t lymphocytes to lymphocyte-conditioned medium (lcm) permits them to divide for longer periods of time, but permanent cell lines cannot be established. intial attempts to grow malignant t cells from patients with cutaneous t-cell lymphomas (ctcl) in unsupplemented growth medium resulted in th ...1979221120
integration of epstein-barr virus dna.the application to herpesviruses of different standard methods fo measuring tumour-virus integration is discussed. the evidence for the presence of integrated virus dna in ebv-transformed cells is summarized.1978221323
herpesvirus papio: state of viral dna in baboon lymphoblastoid cell lines.baboon lymphoblastoid cell lines (lcl) transformed with herpesvirus papio were evaluated for the state of hvp dna in the cells by caesium chloride density-gradient centrifugation and dna-crna filter hybridization. five hvp producer cultures and one non-producer culture were studied and non-integrated and integrated hvp dna were detected in all producer cultures whereas only integrated hvp dna could be demonstrated in the non-producer lcl. non-integrated hvp dna had a density of 1.715--1.716 g/cm ...1978221325
similarities and differences between various herpesviruses: a review. 1978221327
isolation of the epstein-barr virus nuclear antigen from chromatin preparations. 1978221330
epstein-barr virus genomes and their biological functions: a review. 1978221334
studies on epstein-barr virus dna polymerase activities in various human lymphoblastoid cell lines. 1978221344
identification and partial purification of two ebv-associated dna polymerases.virally induced dna polymerases have been demonstrated in cells infected with a variety of herpesviruses. these include herpes simplex virus (weissbach et al., 1973), marek's disease virus (boezi et al., 1974), equine herpesvirus (allen et al., 1977), and cytomegalovirus (huang, 1975a). recently a new iododeoxyuridine (iudr)-induced intracellular dna polymerase in an epstein-barr virus (ebv)-producing hybrid cell line has also been reported (miller et al., 1977). we present evidence that there a ...1978221345
transformation of non-lymphoid cells by herpesviruses: a review. 1978221349
transformation of lymphoid cells by herpesviruses: a review with special reference to the phenotypic properties of transformed cells. 1978221350
mapping of putative transforming sequences of ebv dna.the linkage of restriction enzyme fragments of dna of the b95-8 strain of epstein-barr virus has been determined. two approaches are being employed to define which ebv dna sequences are needed to initiate and maintain the transformation of lymphocytes to lymphoblasts capable of long-term growth in culture. the first approach is to determine the differences between the dna of strains of ebv which possess transforming capacity and the dna of the hr-1 strain which cannot transform. the data indicat ...1978221358
in vitro lymphocyte transformation by epstein-barr virus (ebv)-like viruses isolated from old-world non-human primates.ebv-like viruses and lymphoid cell lines have been isolated from baboons and an orangutan. the cell lines have properties of b- or undifferentiated lymphocytes and have antigens and dna related to those of ebv. the baboon virus has a broad in vitro transformation host range among lymphocytes of old-world simian species whereas the orangutan isolate has a narrower host range. baboon and orangutan viruses as well as ebv have shown transforming activity for gibbon lymphocytes. baboon virus is infec ...1978221359
transcription of epstein-barr virus genomes in human lymphoblastoid cells and in somatic-cell hybrids of burkitt's lymphoma.expression of latent epstein-barr virus genomes in lymphoblastoid cells and somatic-cell hybrid of burkitt's lymphoblastoid cells has been studied. iudr treatment induced the formation of early antigen (ea), virus capsid antigen (vca) and virus dna replication in d98/raji and d98/hr-1 cells whereas only ea was induced in raji cells. hr-1 clone no. 9 did not respond to iudr treatment. the pattern of transcription of virus genomes in these cell lines without iudr treatment was uniform with 20--25% ...1978221360
studies on the association of the epstein-barr virus genome with chromosomes in human (burkitt)/mouse hybrid cells.we have used somatic-cell hybrids of mouse fibroblast and burkitt's lymphoblastoid tumour cells to continue our investigation of the association between epstein-barr virus (ebv) genome and human chromosomes. a mouse/burkitt hybrid cell, designated cl1d/hr-1, was cloned in soft agar. each of 10 clones was assayed for the spontaneous expression of ebv-associated nuclear antigen (ebna), early antigen (ea) and virus capsid antigen (vca). six of 10 clones were ebna-positive but negative for ea and vc ...1978221361
establishment of somatic hybrid cell clones from rat embryonic fibroblast cells with burkitt's lymphoblastoid cells using polyethylene glycol. 1978221362
provisional classification of herpesviruses: herpesvirus study group of the international committee for the nomenclature of viruses. 1978221375
priorities and needs in ebv research. 1978221379
inhibition of epstein-barr virus transformation: evidence for a block between ebna production and cell proliferation.non-adherent lymphocyte populations prepared from donors with previous ebv infection transformed in the usual way in response to ebv. however, transformation was completely inhibited in the presence of adult but not fetal human fibroblasts. such inhibition was associated with lymphocytes from donors with prior ebv infection, but not from those without, and there appears to be an immunological basis for inhibition. inhibition was associated with production of ebna and was reversible. addition of ...1978221385
suppression of in vitro epstein-barr virus infection: a new role for adult human vitro ebv infection of lymphocytes from different sources indicated that b-cells of adult peripheral or fetal cord blood transform with equal efficiency whether assayed by dna synthesis induction or immortalization. unfractionated adult lymphocytes transformed much less efficiently than those from fetal cord blood. this effect was due to the suppression of b-cell proliferation by adult t-lymphocytes.1978221386
surface glycoprotein patterns of human haematopoietic cell lines.human ebv-positive and ebv-negative haematopoietic cell lines were shown to have different and easily distinguishable surface glycoprotein patterns as demonstrated using surface labelling by the galactose oxidase-tritiated sodium borohydride method.1978221389
persistent, chronic, and latent infections by herpesvirus: a review. 1978221391
pathogenesis of infectious mononucleosis, burkitt's lymphoma and nasopharyngeal carcinoma: a unified scheme. 1978221392
antibody-dependent autologous lymphocyte cytotoxicity against cells freshly transformed by epstein-barr virus.cytotoxicity against lymphocytes freshly transformed by ebv was studied in an autologous system by trypan blue exclusion of ebna-positive cells and 51cr-release assay. when ebv-tranformed lymphocytes were incubated with ebv-positive serum and autologous unfractionated lymphocytes or k-cells from sero-positive healthy donors, the transformed cells were significantly damaged. the cytotoxicity against newly produced ebna-positive lymphocytes was also remarkable when sero-positive healthy donor lymp ...1978221393
inhibition by phosphonoacetate of the in vitro outgrowth of epstein-barr virus genome-containing cell lines from the blood of infectious mononucleosis patients. 1978221394
detection of epstein-barr virus-coded antigens in lymphocytes isolated from defined patient samples.a preliminary report on the use of specific rabbit antisera raised to epstein-barr virus-coded antigens (ebna and ea) for detection of these antigens in vivo is presented. human lymphocytes were isolated on isokinetic gradients and the c3 receptor-bearing b-lymphocyte subpopulation was isolated, providing an enriched source of ebv-infected lymphocytes. such technology was employed to establish the status of the ebv host-cell complex in recurrent exudative tonsillitis (ret), infectious mononucleo ...1978221395
epstein-barr virus nuclear antigen (ebna)-positive cells in a lymph-node of a child with severe primary ebv infection. 1978221396
replication of epstein-barr virus dna in epithelial cells in vivo.epstein-barr virus (ebv)-specific complementary rna (crna) was hybridized in situ to oropharyngeal epithelial cells taken from patients with infectious mononucleosis. cells from patients shedding virus in the throat hybridized signifnicant quantities of crna, whereas cells from ebv-negative sources did not. the degree of hybridization indicated a large ebv genome number per infected epithelial cell and suggested that these cells were the source of virus found in the throat. this finding may expl ...1978221397
comparison of immune responses and viral markers in herpesviruses-associated carcinomas: a review. 1978221405
comparison of humoral and cell-mediated responses and virus markers in herpesvirus-associated lymphomas: a review. 1978221406
"neonatal" tumour induction: the emerging immune surveillance mechanism and amphibian embryo tumorigenesis. 1978221407
evb-associated immune complexes and recurrent burkitt's lymphoma. 1978221409
iga and nasopharyngeal carcinoma. 1978221410
evaluation of delayed hypersensitivity reactions to lymphoid cell lines and antibodies to epstein-barr virus. 1978221411
viral exposure and abnormal liver function in haemophilia.several studies have recently documented the presence of persistently abnormal liver function tests in asymptomatic haemophiliacs. while the aetiology is unknown it is possible that repeated exposure to agents transmitted in blood products may be important. this study has attempted to determine the prevalence of viral exposure and its relationship to liver function in this multitransfused group of individuals. the prevalence of viral antibodies with the exception of antibody to hepatitis b surfa ...1979221550
polyclonal immunoglobulin secretion by human b lymphocytes exposed to epstein-barr virus in vitro. 1979221572
lymphocyte activation by the tumor-promoting agent 12-o-tetradecanoylphorbol-13-acetate (tpa).tpa, a highly active tumor-promoting agent, is an effective mitogen for primate peripheral blood lymphocytes. optimal stimulation of human lymphocytes was obtained 4 days after the addition of tpa at a concentration of 7.5 ng/ml. lymphocyte fractionation experiments demonstrated that both t and b cells incorporated 3h-thymidine significantly in response to tpa. lymphocyte blastogenesis was not due to the reactivation of latent herpesviruses by the tumor promoter, since similar responses to tpa w ...1979221578
the effects of mitogens on the expression of epstein-barr virus antigens in human lymphoid cell lines.treatment of human lymphoblastoid cells with either phytohemagglutinin (pha), concanavalin a, staphylococcus protein a, or polyinosinic acid-polycytidylic acid, in combination with 5-iodo-2' deoxyuridine (iudr) markedly increased the expression of epstein-barr virus (ebv) early antigen (ea) relative to iudr alone. such treatment did not, however, modify the production of virus capsid antigen in any of the lymphoid cell lines tested. the effect of pha on ea induction in raji cells was not accompa ...1979221589
cross-neutralization of infectious mononucleosis and burkitt lymphoma strains of epstein-barr virus with hyperimmune rabbit antisera. 1979221591
incidence of heterophil antibody responses in children with infectious mononucleosis. 1979221629
the epstein-barr virus and eb virus infections in childhood. 1979221638
[establishment of leukaemia cell lines and their significance for clinical and experimental oncology (author's transl)].permanent in vitro growing leukemic cell lines have been established from all types of immunologically classified childhood leukemias. essential characteristics of primary blasts and cultured cells are identical. in contrast to lymphoblastoid, non-leukemic cell lines, the epstein-barr-virus specific nuclear angiten (ebna) is not detected. up to now 8 non-b-non-t cell lines (6 of them were derived from children with acute lymphoblastic leukemia, 2 from patients with chronic myeloid leukemia), 8 t ...1979221724
tumour promoter tpa enhances transformation of human leukocytes by epstein-barr virus. 1979221841
practical approach to diagnosis of infectious mononucleosis. 1979221896
detection of a nuclear antigen in herpesvirus ateles-carrying marmoset lines by the acid-fixed nuclear binding vitro binding of a herpesvirus ateles (hva)-associated soluble antigen to amphibian erythrocyte nuclei was demonstrated by the acid-fixed nuclear binding technique in combination with anticomplement immunofluorescence. incubation of concentrated salt-extracted soluble antigens derived from hva-carrying marmoset lines with methanol/acetic acid-fixed erythrocytes of frogs and salamanders resulted in a brilliant nuclear fluorescence after exposure to a live virus-boostered, anti-hvs-positive squ ...1979221913
lymphoma development in mice and humans: diversity of initiation is followed by convergent cytogenetic evolution.human b cell lymphoma and murine t cell leukemia can be initiated by several agents. the present paper formulates some thoughts on the role of cytogenetic changes in the subsequent neoplastic process. initiation creates long-lived preneoplastic cells. in some respects, they are comparable to in vitro-transformed ("immortalized") cell lines that maintain a diploid karyotype and are not tumorigenic in vivo. the development of a tumorigenic ("autonomous") clone is dependent on additional changes at ...1979221925
the epstein-barr virus. 1979221971
alterations in cell characteristics in relation to malignant transformation. 1979222016
persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). 1979222414
the ebv-carrying, beta2m/hla deficient burkitt lymphoma line daudi is sensitive to ebv-specific killer t-cells of mononucleosis patients.the beta2-microglobulin/hla deficient burkitt lymphoma line daudi was tested for sensitivity to ebv-specific cytotoxicity mediated by natural killer (nk)-depleted t-cells from acute mononucleosis patients. while the daudi line was not as sensitive as the reference ebv-genome-positive target line, it was clearly sensitive in the majority of cases. this would speak against a major role of syngeneic restriction in this system.1979222436
epstein-barr virus rna in burkitt tumor tissue. 1979222455
induction of plaque-forming cells in human blood lymphocytes cultured in the presence of antigen and epstein-barr virus: a study with normal donors and infectious mononucleosis patients.normal human peripheral blood lymphocytes, stimulated in vitro with srbc in the presence of epstein-barr virus (ebv), gave rise to plaque-forming cells (pfc) specific for the antigen. pfc levels were very low before day 4 and increased thereafter, reaching a maximum around day 8. however, the kinetics of the response varied considerably from donor to donor and from experiment to experiment. in some instances a second peak of pfc was obtained beyond day 10. large differences in the magnitude of t ...1979222519
development of antibodies reactive in antibody-dependent cellular cytotoxicity in infectious mononucleosis.serial sera from patients with infectious mononucleosis were examined for the emergence of antibodies reactive in antibody-dependent cellular cytotoxicity tests, using epstein-barr virus-superinfected raji cells as targets. for this specific purpose, the antibody-dependent cellular cytotoxicity test proved to be of limited sensitivity because only relatively high serum dilutions can be tested dependably, due to prozone effects at low serum concentrations, and because antibody-dependent cellular ...1979222678
persistent infection of a human lymphoblastoid cell line with equine herpesvirus 1.infection of a human lymphoblastoid cell line (jijoye line derived from a burkitt lymphoma which contains epstein-barr virus) with equine herpesvirus 1, maintained and observed for 53 days, was characterized by the continuous production of infectious extracellular and intracellular virus. maximum virus production correlated with active cell multiplication. less than 15% of the cells possessed viral capsid antigen at any one time. five percent of the cells in the jijoye line possess epstein-barr ...1979222688
long-term c-cell-mediated immunity to epstein-barr virus in man. ii. components necessary for regression in virus-infected leukocyte cultures.regression of eb-virus-induced transformation occurs exclusively in cultures of leukocytes from seropositive donors. studies have shown that the strength of regression could be assayed in terms of the proportion of t cells which must be added to the autologous eb virus-infected t-cell-depleted population in order to establish regression in the culture. the in vitro regression phenomenon was strongly t-cell-dependent but did not require the presence of either monocytes or, as a potential antigeni ...1979222690
long-term t-cell-mediated immunity to epstein-barr virus in man. iii. activation of cytotoxic t cells in virus-infected leukocyte cultures.experiments have been conducted to determine the role played by immune t cells in the regression of eb-virus-induced transformation which is exclusively seen in leukocyte cultures from sero-positive donors. kinetic studies suggest that, in virus-infected cultures from such donors, a population of t cells proliferates within the first 2 weeks apparently in response to the appearance of virus-infected b cells. this proliferation continues to some extent during the period of regression. nonspecific ...1979222691
[herpesviruses in man].the importance and the extent of the inter-actions between herpes viruses and man is beginning to be understood. as more is learned about the natural histories of these viruses, it is becoming apparent that they have in common: horizontal spread during childhood, often with subclinical infection. latency in the host for many years, probably for life. potential for reactivation at a later time. host immune response maintained for life. potential for oncogeneiss. a more thorough knowledge of the b ...1979222711
pleocytosis and immunoglobulin changes in cerebrospinal fluid and herpesvirus serology in patients with guillain-barré a follow-up study of 24 patients with guillain-barré syndrome, 55% developed a cerebrospinal fluid (csf) mononuclear pleocytosis, which persisted for 4 months or more. raised index values of csf-immunoglobulin g (igg), csf-iga, and csf-igm, indicating synthesis of the immunoglobulin in question in the central nervous system, were found in 63, 35, and 25%, respectively. agarose gel eletrophoresis revealed oligoclonal immunoglobulin in csf in 21% and in both csf and serum in another 21% of the ...1979222797
simultaneous radioimmunoassay for specific antibodies to members of the human herpesvirus group.a method of described for the simultaneous radioimmunoassay (ria) for antibody to members of the human herpesvirus group. the ria is compared with some of the conventional serologic techniques used to quantitate antibody to these viruses (epstein-barr virus, cytomegalovirus, herpesvirus type 1 and varicella-zoster virus). color-coded beads, each coated with the antigens of a different herpesvirus, were similtaneously placed in a well which contained a human serum to be assayed for antibody to ea ...1979222846
[eb virus and lymphocytes]. 1979222920
theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: iv. the viruses.speculations are developed for a mechanism by which oncogenic viruses can induce alterations in cells allowing them to express embryonic genes. it is suggested that if viral deoxyribonucleic acid, directly or via ribonucleic acid directed deoxyribonucleic acid polymerase activity becomes inserted at particular euchromatin - heterochromatin junctions of quasidifferentiated stem-like cells, then deheterochromatization may result, causing in turn derepression of genes for acidic protein phosphokina ...1979223021
[the role of herpes and papilloma viruses in human tumors]. 1979223047
mitogenic stimulation of leukemia cells by epstein-barr virus. 1979223056
long regions of single-stranded dna in human cells. 1979223063
infectious mononucleosis. 1979223111
sites of sequence variability in epstein-barr virus dna from different sources.the intracellular epstein-barr virus (ebv) dna present in virus-transformed cells was partly purified from 23 cell lines or biopsies of burkitt lymphoma, nasopharyngeal carcinoma, infectious mononucleosis, or healthy carrier origin. such dna was cleaved in fragments (a-k) of molecular weights between 1 x 10(6) and 30 x 10(6) with restriction enzyme ecori, and these fragments were analyzed by standard methods involving agarose gel electrophoresis, transfer to nitrocellulose filters, and hybridiza ...1979223159
a live human b-cell activator operating in isolation of other cellular influences.using a reversed haemolytic plaque assay, we demonstrate that live but not inactivated epstein-barr (eb) virus can induce human blood lymphocytes to polyclonal ig (igm and igg) secretion in vitro. unlike pokeweed mitogen this b-cell-activating effect takes place in relative absence of t cells. eb virus thus promises to be a tool for study of b cell function isolated from other cellular influences, although its mechanism of activation appears to be different from that of previously known b cell a ...1979223232
recovery of epstein-barr virus from nonproducer neonatal human lymphoid cell transformants. 1979223286
the establishment of the suspension epstein-barr virus producing cell lines from patients with tumoral diseases.isolation of the epstein-barr virus (ebv) in suspension lymphoblastoid cell lines from human patients with tumor diseases, mainly malignant lymphoma, has been described. it has been shown that the ebv was isolated from human patients with myeloid type of leukemia in 75% of cases. a similar virus was also isolated from patients with hodgkin's disease and leukemoid reaction of the myeloid type for lung cancer. morphological, cytochemical, immunological, and cytogenetic characteristics of the cell ...1979223867
expression of differentiated functions in hybrid cells. 1979223931
[studies on epstein barr virus (ebv)-related antigens: biochemical characterization and purification of ebv-determined nuclear antigen (ebna) (author's transl)]. 1979223952
triplex gene dosage effect of tpi and g3pd in a human lymphoblastoid cell line with partial trisomy 12p13 and 18p.the ebv-induced lymphoblastoid line established from a patient carrying a duplication of the distal part of chromosome 12 short arm (12p13) retained the original partial trisomy and displayed the same triplex gene dosage effect for tpi and g3pd as found in the patient's rbc and wbc.1979223966
simultaneous presence of ebna-positive and colony-forming cells in peripheral blood of patients with infectious mononucleosis.ebna-positive lymphoblast cells were detected in 0.1 to 0.9% of the t-cell-depleted lymphocytes obtained from peripheral blood samples of five patients with infectious mononucleosis (im). the same blood specimens from four of the five patients contained cells that formed ebna-positive colonies in soft agar containing ebv antibodies. the ratio of the colony formers to ebna-positive cells was higher in blood samples taken early in the disease than in those obtained in later stages of the disease. ...1979223990
relationship between the epstein-barr virus genome and nasopharyngeal carcinoma in caucasian order to explore whether undifferentiated nasopharyngeal carcinoma (npc) shows a regular association with epstein-barr virus (ebv), regardless of the geographical and ethnic origin of the patient, a correlated histopathological and nucleic acid hybridization study was performed on biopsies from caucasian patients with nasopharyngeal carcinomas and from various controls. among 12 undifferentiated npcs, 11 were positive for ebv-dna, with multiple copies of the viral genome per cell. serological ...1979223991
light and electron microscopic investigations of nasopharyngeal carcinomas with regard to the viral etiology of these tumors.five carcinomata of the nasopharynx (four lymphoepithelial carcinomata of the regaud type and one squamous cell carcinoma) were examined light and electron microscopically. in addition to the familiar histological and cytological features of these tumors, and because of an increased antibody titer against epstein-barr virus in all five patients, all those cytoplasmic and nuclear inclusions were examined which could be interpreted as indicative of a virus contact. the following structures were fo ...1979224065
[c3 receptor expression in lymphocytes]. 1979224224
[infantile cortical hyperostosis. description of a case with positive paul-bunnell-davidsohn reaction]. 1978224298
continuous culture of t cells cytotoxic for autologous human leukaemia cells. 1979224318
ebv-specific humoral antibodies in nasopharyngeal carcinoma patients in cuba.sera of 24 patients with nasopharyngeal carcinoma (npc) and of 60 healthy donors were studied for presence of igg and iga antibodies against different antigens induced by epstein--barr virus (ebv). the results obtained demonstrate that npc in cuba, as in other countries, is accompanied by elevated antibody titers against ebv-specific igg antibodies, whose levels strongly differ from those of healthy persons. patients with npc in cuba had higher titers of antibody against vca of ebv in comparison ...1979224330
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