| enzyme-linked immunosorbent assay for detection of virus-specific igm antibodies to varicella-zoster virus. | a sensitive enzyme-linked immunosorbent assay (elisa) is described for detection of varicella-zoster virus (vzv) igm antibodies. the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. the tested sera were absorbed with staphylococcus aureus (strain cowan i) before analysis. rabbit anti-human igm peroxidase conjugate was used to detect human igm bound to viral antigen. the results were compared with those obtained by the indirect fluorescent antibody to membran ... | 1980 | 6248482 |
| [facial paralysis and varicella-zoster virus (vzv): serum antibody titer and vzv skin test (author's transl)]. | | 1980 | 6248622 |
| transfer factor for the prevention of varicella-zoster infection in childhood leukemia. | sixty-one patients with leukemia and no immunity to chickenpox were given dialyzable transfer factor or placebo and followed for 12 to 30 months in a double-blind trial designed to examine the clinical efficacy of transfer factor. sixteen patients in the transfer-factor group and 15 in the placebo group were exposed to varicella zoster, and most of them had a rise in antibody titer. chickenpox developed in 13 of 15 exposed patients in the placebo group but in only one of 16 in the transfer-facto ... | 1980 | 6248780 |
| therapeutic potential of transfer factor. | | 1980 | 6248781 |
| corticosteroids and varicella disciform keratitis. | | 1980 | 6249122 |
| active immunizations for adults. | | 1980 | 6249188 |
| 2'-fluoro-5-iodo-aracytosine, a potent and selective anti-herpesvirus agent. | a newly synthesized pyrimidine analog, 2'-fluoro-5-iodo-aracytosine (fiac), suppressed by 90% the replication of various strains of herpes simplex virus types 1 and 2 at concentrations of 0.0025 to 0.0126 microm. cytotoxicity was minimal, as determined by trypan blue dye exclusion with norman vero, wi-38, and nc-37 cell proliferation; the 50% inhibitory dose was 4 to 10 microm in a 4-day assay. when compared with other antiviral drugs, fiac was active at much lower concentrations than arabinosyl ... | 1980 | 6249196 |
| virus replication and localization of varicella-zoster virus antigens in human embryonic fibroblast cells infected with cell-free virus. | when human embryonic fibroblast cells were infected with cell-free varicella-zoster virus, virus replication began between 8 and 14 h postinfection, and 4 more h werp required for the virus to infect neighboring cells. virus-specific antigens were traced by the anticomplement immunofluorescent antibody technique. virus antigen was first detectable 2 h postinfection in the cytoplasma, and diffuse fluorescence was observed in the nucleus as early as 4 h after infection. the nuclear fluorescence go ... | 1980 | 6249744 |
| comparative in vitro imunotoxicology of acyclovir and other antiviral agents. | in vitro lymphocyte blastogenic responses to the commonly employed mitogens phytohemagglutinin, pokeweed, and concanavalin a were evaluated when acyclovir, adenine arabinoside, cytosine arabinoside, and idoxuridine were added to the culture materials. similarly, specific antigen-induced blastogenic responses, including herpes group antigens, and cytotoxicity and leukocyte inhibitory factor assays with herpes group viruses were determined in the presence and absence of antiviral agents. no depres ... | 1980 | 6249751 |
| varicella vaccination: where do we go from here? | although meager knowledge of the virus raises serious safety questions, the risk of death from varicella in immunocompromised children justifies further trials. in the meantime, passive immunization is a safe and relatively effective alternative. | 1980 | 6250972 |
| varicella morbidity in czechoslovakia. | data are presented on varicella and herpes zoster morbidity notified in czechoslovakia in the years 1970 to 1978. the notified varicella incidence is compared with serologically confirmed varicella incidence among the selected groups of children up to the age of 12 from the north-moravia region. comparative analysis revealed a considerable difference between the notified and serologically detected cases of varicella. the highest rate of notified varicella was recorded in children of 3 and 4 year ... | 1980 | 6251130 |
| [facial paralysis and varicella-zoster virus (vzv); fluctuations in anti-vzv antibody titers and early diagnosis by viral specific igm antibody examination (author's transl)]. | | 1980 | 6251192 |
| classics in infectious diseases. the cultivation of the poliomyelitis viruses in tissue culture by john f. enders, frederick c. robbins, and thomas h. weller. | | 1980 | 6251529 |
| isolation and polypeptide characterization of varicella-zoster virus. | | 1980 | 6251617 |
| skin test with varicella-zoster virus antigen on herpes zoster patients. | intracutaneous injection of varicella-zoster (v-z) viral antigen was performed on 55 patients with herpes zoster. all of them reacted positively after 10 days. the patients in the acute stage responded weakly wereas those in the convalescent phase developed a stronger reaction. the intensity of the reaction did not correlate with the number of days of illness. positive reactions were observed in those patients who had suffered from herpes zoster several years ago. complement fixing antibody tite ... | 1980 | 6251751 |
| viral infection and acute peripheral facial palsy. a study with herpes simplex and varicella zoster viruses. | the role of herpes simplex virus (hsv) and varicella zoster virus (vzv) in acute peripheral facial palsy (apfp) was evaluated according to clinical symptomatology and serologic findings in a series of 70 patients seen over a one-year period. peak morbidity of apfp occurred in the 21 to 30 years age group. most patients were under 40 years of age and there were more female than male patients. concomitant dysfunction of one or more other cranial nerves was present in most patients. the sera of pat ... | 1980 | 6252119 |
| solid-phase enzyme immunoassay for determination of antibodies to cytomegalovirus. | a solid-phase enzyme immunoassay for the determination of immunoglobulin h (igg) and igm antibodies to cytomegalovirus is described. the enzyme immunoassay gave reliable and consistent results which were in concordance with those obtained by the complement fixation test and the indirect immunofluorescence test. antibodies to herpes simplex and varicella-zoster viruses did not interfere in the enzyme immunoassay for cytomegalovirus igm antibodies. in a few patients with igm antibodies to epstein- ... | 1980 | 6252241 |
| [clinical aspects of chronic virus infections]. | | 1980 | 6252452 |
| in vitro susceptibility of varicella-zoster virus to acyclovir. | the in vitro susceptibility of five strains of varicella-zoster virus to acyclovir was examined by the plaque-reduction method in human diploid lung cells. the 50% effective doses of acyclovir ranged from 2.06 microm to 6.28 microm in a 7-day assay, with a mean of 3.65 microm. irreversible inhibition of plaque formtation was achieved by drug doses exceeding the 50% effective dose for plaque reduction but nontoxic to the cells. studies on the relative in vitro susceptibility of varicella-zoster v ... | 1980 | 6252836 |
| comprehensive viral immunology of multiple sclerosis. ii. analysis of serum and csf antibodies by standard serologic methods. | sera and csfs of 85 patients with multiple sclerosis (ms), 49 patients with probable ms, and 165 control patients with other neurologic diseases were assayed for antibodies to rubella, mumps, measles, parainfluenza i (strain 6/94), herpes simplex, cytomegalovirus, varicella-zoster, and vaccinia viruses. methods included complement fixation (cf), hemagglutination inhibition (hi), and complement-dependent plaque reduction (cpr). significant differences between the groups with ms and the control gr ... | 1980 | 6252874 |
| comprehensive viral immunology of multiple sclerosis. iii. analysis of csf antibodies by radioimmunoassay. | the csf from 279 patients with multiple sclerosis (ms), probable ms, or controls was examined by radioimmunoassay (ria) for antibodies to measles, rubella, mumps, parainfluenza 1 (sendai) (strain 6/94), herpes simplex (hsv), varicella, and vaccinia viruses. significantly more patients with ms than noninflammatory control patients had antibody to measles, rubella and varicella viruses, of which antibody to measles was the most prevalent. the percentage of patients with ms with two or more csf ant ... | 1980 | 6252875 |
| herpes zoster in a husband and wife. | the wife of a 75-year-old man with zoster began to suffer from this disease some 24 days after is appeared in her husband. although zoster is generally regarded to be an endogenously noncommunicable disease, the case reported herein suggests that the infection was exogenously communicated. the literature pointing up that zoster may possibly be acquired exogenously, particularly in immunosuppressed patients, is extensively reviewed. | 1980 | 6253406 |
| cryopreservation of virus-infected cells for use in the fluorescent antibody to membrane antigen test. | tissue culture cells infected with varicella-zoster, respiratory syncytial, para-influenza types 1, 2, and 3, and influenza a/texas/1/77 and a/ussr/90/77 viruses were exposed to ultraviolet light and frozen in the presence of a final concentration of 20% glycerol. these cells were quick thawed at 37 degrees c and compared with freshly prepared, living infected tissue culture cells in assays of fluorescence antibodies to membrane antigens of the infecting agents in serum, nasal wash secretions, c ... | 1980 | 6253517 |
| [viral neuraxitis. further studies of the current epidemiological and clinical aspects]. | an epidemiological and clinical investigation upon ten cases of acute viral neuraxitis observed in the country of alessandria between january 1978 - january 1979, is reported. four cases of neuraxitis were due to coxsackie b4 virus, two to coxsackie b3, two to influenza type a and one to influenza type b virus, one to rubella and one to varicella-zooster virus. in all the cases blood chemistry, clinical and instrumental examinations (eegraphy, cerebral scintigraphy and angiography) were coupled ... | 1980 | 6253851 |
| [radioprotective effectiveness of the paramunity inducer "pind-avi": investigation using the model "protective tetanic vaccination in mice" after exposure to x-rays (author's transl)]. | the model "protective tetanic vaccination in mice" was used in order to try whether the paramunity inducer pind-avi may diminish or suspend radiation-induced immunosuppression. the radioprotective effect was ascertained from the different results of immunization in mice exposed to radiation after pretreatment with either pind-avi or placebo. the dose delivered amounted to 300 r. tetanus toxoid was used for immunization. effectiveness of the tetanic vaccination was analysed by means of load tests ... | 1980 | 6254210 |
| an outbreak of varicella-zoster virus infection among cancer patients. | an outbreak of varicella-zoster infection occurred among patients of the medicine branch, national cancer institute, national institutes of health. epidemiologic investigation suggested that the outbreak was due to two distinct types of disease. one type was acquired without previous exposure to other diseased patients and invariably associated with dermatomal lesions. the other, an a typical form, was associated with person-to-person transmission and equivocal initial dermatomal distribution an ... | 1980 | 6254415 |
| viral and bacterial antibody responses in multiple sclerosis. | an imprint electroimmunofixation method (ieif) was used to characterize antibodies to eight viral antigens (measles, mumps, rubella, herpes simplex type 1, varicella-zoster, vaccinia, cytomegalovirus, adenovirus) and four bacterial antigens (beta-hemolytic streptococcus, hemophilus influenzae type b, escherichia coli, enterococcus) in serum and cerebrospinal fluid (csf) of 12 patients with multiple sclerosis (ms). twelve patients matched for age and sex sex served as controls. evidence for intra ... | 1980 | 6254433 |
| immune response of guinea pigs to varicella vaccine strain (oka) and wild strains. | an immune response (neutralizing and complement fixing antibodies) was detected in guinea pigs to varicella vaccine strain (oka), but not to 2 wild strains. the possible use of guinea pigs and the vaccine strain (oka) as a model system is discussed. | 1980 | 6254499 |
| the age distribution of neutralizing antibodies against varicella-zoster virus in healthy individuals. | a seroepidemiological survey of varicella was made in the nagoya area by the neutralization (nt) test. of 1,473 recorded cases of varicella, 81.4% were under 6 years old and 9.6% were under one year old; of the 168 recorded cases under one year old, about 30% were under 5 months old. examination of 11 pairs of mother and cord sera and 13 pairs of mother and infant sera showed that transfer of nt antibody was in general good, even in babies that were small for their age or smnall at birth after 2 ... | 1980 | 6254500 |
| experimental infection of guinea pigs with varicella-zoster virus. | weanling guinea pigs are susceptible to infection with varicella-zoster virus (vzv). animals inoculated intranasally or subcutaneously with vzv grown in fetal guinea pig tissue culture shed virus from the nasopharynx and seroconverted to vzv. viremia occurred in some animals. animal-to-animal transmission of vzv was observed. infection of weanling guinea pigs with vzv should allow assessment of the pathophysiology of viral infection in immunocompetent and specifically immunologically modified an ... | 1980 | 6255038 |
| primary epstein-barr virus infection in association with reye syndrome. | | 1980 | 6255120 |
| impact of hodgkin's disease upon the immune system. | | 1980 | 6255607 |
| rapid random-donor screening for the selection of viral antibody-containing blood donations. | we describe a 3 1/2 year trial in which we used immunofluorescence to detect and titrate antibody to six common viruses in normal healthy blood donors drawn at random from west and south east scotland. the trial was designed to allow selection of blood donations rich in specific antiviral antibody for inclusion in the plasma pool which would, in turn, be used for the preparation of specific antiviral immunoglobulin. the results show the natural frequency of occurrence of antibody to different vi ... | 1980 | 6255854 |
| recurrent herpes zoster encephalitis. a complication of systemic lupus erythematosus. | a middle-aged woman had five discrete episodes of herpes zoster. the first attack consisted of uncomplicated herpes zoster ophthalmicus. the subsequent four episodes involved thoracic, cervical, and finally sacral dermatomes and were complicated by myelitis or encephalomyelitis. during the most recent attack, while she was receiving corticosteroids, varicella-zoster virus was cultured from the csf. in addition, the patient had strong evidence of systemic lupus erythematosus, with a history of ra ... | 1981 | 6257212 |
| prevention of varicella in urgent cases by passive transfer of vaccine-induced immunity. | for the purpose of preventing spread of infection to high risk children whose immunities were severely impaired by intensive chemotherapy or for some other reason, when cases of varicella occurred in a children's ward or in a family, healthy adults (mothers and a doctor) were immediately given live varicella vaccine, blood was collected from these adults 5 to 7 days after vaccination and the whole blood or plasma including the buffy coat was transferred in the high risk children. subsequently th ... | 1980 | 6257226 |
| humoral and cellular immune response to varicella-zoster virus in children inoculated with live attenuated varicella vaccine. | twenty-seven hospitalized children without a history of varicella were vaccinated with 500 plaque forming units of live attenuated varicella vaccine (oka strain) and followed for four to five weeks at weekly intervals for the development of virus-specific cell-mediated immunity (cmi) and neutralizing (nt) antibody activity. these children had suffered from heterogeneous underlying diseases, such as acute leukemia, lymphoma, the nephrotic syndrome, and other chronic illnesses and were in various ... | 1980 | 6257227 |
| spread of varicella in hospitalized children having no direct contact with an indicator zoster case and its prevention by a live vaccine. | varicella spread from a child with zoster to a total of 3 susceptible infants in another room in a children's ward, although they had been strictly isolated. to prevent spread of the disease, the staffs and patients were doing their own washing and no source of natural infection could be found. the cases indicate that it is difficult to predict nosocomial varicella infection or to prevent spread of the disease simply by isolation in a children's ward. a total of 11 other children without history ... | 1980 | 6257228 |
| studies on the polypeptides of varicella-zoster (v-z) virus. ii. syntheses of viral polypeptides in infected cells. | synthesis of varicella-zoster virus (vzv)-induced polypeptide was examined at intervals after infection by immunoprecipitation, sds-polyacrylamide gel electrophoresis (page) and fluorography. human embryonic lung (hel) cells were inoculated with vzv-infected cells at a ratio of about 1 infected cell to 5 uninfected cells and pulse-labelled with 35s-methionine or 14c-glucosamine. under this experimental condition, virus-induced protein and glycoprotein began to appear 4 to 6 and 18 to 20 h after ... | 1980 | 6257229 |
| [changes in antibody titre after rubella vaccination in primarily rubella-sera positive parturient women. demonstration of antigen-specific (rubella) and non-specific stimulated antibody titres (author's transl)]. | | 1980 | 6257468 |
| varicella-zoster virus transformation of hamster embryo cells. | varicella-zoster virus infection of primary hamster embryo cells resulted in oncogenic transformation. these transformed cells exhibited virus-specific antigens by immunofluorescence and developed surface fc receptors. they induced aggressive fibrosarcomas when injected into inbred hamsters. the tumour-bearing hamsters develop antibodies to varicella-zoster antigens. | 1980 | 6257832 |
| radiology in inflammatory disease of the colon: an area of increased interest for the modern clinician. | | 1980 | 6259078 |
| clinical and laboratory studies of kmcc strain live attenuated varicella virus. | | 1981 | 6259644 |
| new developments with new vaccines. | | 1980 | 6259664 |
| [clinico-pathogenetic variants of peripheral nervous system diseases of herpetic etiology]. | of 340 patients with various manifestations of herpetic disease, 261 showed predominant involvement of the peripheral nervous system. apart from ganglioneuritis of the cranial and spinal nerves there occurred forms accompanied by inflammatory changes in the liquor, commissural processes in the meninges, involvement of the anterior roots and vegetative ganglions. the results of clinicovirological comparisons and differentiation of herpetic diseases as well as their initial manifestations are repo ... | 1980 | 6259855 |
| [an immunological study of herpes zoster patients iii--specific immunity (author's transl)]. | | 1981 | 6260390 |
| immune response after exposure to varicella zoster virus: characterization of virus-specific antibodies and their corresponding antigens. | fourteen varicella zoster virus antigens were identified that induce antibodies during primary and recurrent infections. these antigens, which included the major nucleocapsid polypeptide (molecular weight, 155,000) and three glycoproteins (molecular weights, 130,000, 88,000, and 60,000, respectively) plus a number of minor antigens, were identified in radioimmunoprecipitation assays, using [35s]methionine-labeled extracts of cells infected with varicella zoster virus and sera from patients with ... | 1981 | 6260665 |
| epstein-barr virus antibodies in children with atopic disease. | the prevalence and titers of antibodies to human herpetoviruses as well as rubella and mumps viruses were determined in 98 children with atopic diseases and in 55 nonatopic control children. the only significant difference between the two groups was found as regards epstein-barr virus (ebv) antibodies, which were found in higher titers in atopic children of all ages. increased levels of ebv antibodies were found both in children with asthma and atopic dermatitis. the frequency of seropositivity ... | 1981 | 6260689 |
| immunoglobulins m and g to varicella-zoster virus measured by solid-phase radioimmunoassay: antibody responses to varicella and herpes zoster infections. | both immunoglobulin m (igm) and igg antibodies to varicella-zoster virus (vzv) were detectable in a solid-phase radioimmunoassay with 125i-labeled goat antisera to human immunoglobulins. primary infection with vzv was associated with early production of igm and igg antibodies and rapid development of lymphocyte transformation to vzv antigen. among eight subjects with varicella tested 1 to 4 days after onset, seven patients had igg and six patients had igm antibodies; all patients had both igg an ... | 1980 | 6260833 |
| rapid diagnosis of varicella-zoster virus infections by countercurrent immunoelectrophoresis. | a simple, accurate, and rapid method for laboratory diagnosis of varicella-zoster virus (vzv) infections using countercurrent immunoelectrophoresis (cie) is described. cie uses zoster convalescent-phase serum to detect vzv antigen in vesticular fluid. eighty-six patients were studied, 58 with vzv infections and 28 with vesicular or bullous rashes due to causes other than vzv infection. all patients with documented vzv infection had positive cie tests for vzv, and the controls were uniformly nega ... | 1981 | 6260875 |
| septic and aseptic arthritis complicating varicella. | | 1981 | 6260891 |
| [varicella-zoster virus infection in children with leukemia]. | | 1980 | 6261111 |
| multifocal varicella-zoster virus leukoencephalitis temporally remote from herpes zoster. | two patients with cancer, one with hodgkin's disease and the other with a granulosa cell tumor of the ovary, developed a progressive, eventually fatal infection of the central nervous system exhibiting multifocal symptoms and signs. pathologically, gross abnormalities of the brain resembled those in progressive multifocal leukoencephalopathy (pml), with discrete and confluent plaque-like lesions concentrated in the white matter, particularly along the gray-white junction. microscopically, pathol ... | 1981 | 6261672 |
| enhancement of varicella-zoster virus replication in cultured human embryonic lung cells treated with the pesticide carbaryl. | in studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (vz) virus-infected human embryonic lung cells with the pesticide carbaryl. the replication of the cell-associated vz virus was enhanced 2- to 13-fold as compared to control cultures in sevin 4 oil-treated cultures and in cultures treated with the pesticide's active ingredient, carbaryl. the replication of vz virus in cultures teated ... | 1981 | 6261690 |
| immunogenic glycoproteins of laboratory and vaccine strains of varicella-zoster virus. | high-titered antisera were prepared in guinea pigs and rabbits against two strains of varicella-zoster virus (vzv): vzv-32, a low-passage laboratory strain, and vzv-oka, a vaccine strain attenuated by passage in both human and guinea pig embryo cells. when the animal vzv-immune sera, as well as a human zoster serum, were used to precipitate radiolabeled glycoproteins from vzv-infected cells and the immune precipitates were analyzed by polyacrylamide gel electrophoresis and fluorography, it was o ... | 1981 | 6262245 |
| an interchangeable elisa for cytomegalovirus antigen and antibody. | an enzyme-linked immunosorbent assay (elisa) was developed to demonstrate cytomegalovirus (cmv) antibodies and antigen. a nuclear antigen from cmv-infected cells was used for detection of igg and igm antibodies. significant rises of cmv-igg and significant levels of cmv-igm were found in sera from patients with acute cmv only, and not with other herpesvirus infections. cmv antigens were demonstrated in cells and in culture medium by a sandwich or by an inhibition elisa technique. in the sandwich ... | 1981 | 6262352 |
| detection of human cytomegalovirus-specific igg antibodies by a sensitive solid-phase radioimmunoassay and by a rapid-screening test. | a radioimmunoassay (ria) for the detection of human igg antibodies to cytomegalovirus (cmv) which utilizes extracts of cmv-infected cells as antigen is described. sera of 66 healthy adults were titered by the ria and by the complement fixation (cf) test. these same subjects were also used in the evaluation of a rapid screening radioimmunoassay (rsria) developed to detect igg antibodies to cmv in small serum samples. the specificity of the ria was evaluated by testing sera of seven cmv patients a ... | 1980 | 6262448 |
| anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy. | e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ... | 1980 | 6263181 |
| soluble skin test antigen of varicella-zoster virus prepared from the fluid of infected cultures. | a soluble varicella virus antigen for skin testing was developed using culture fluid from varicella-zoster virus-infected human diploid cells. the culture fluid was centrifuged at a high speed (100,000 g for 2 hr) to remove viral particles, and the supernatant, after heating at 56 c for 30 min, was used as the soluble skin test antigen. no virion or nucleocapsid was found by electron microscopy, and very little 3 h-labeled dna in the soluble antigen hybridized with viral dna. by immunoprecipitat ... | 1981 | 6263986 |
| a rapid radioimmunoassay using 125i-labeled staphylococcal protein a for antibody to varicella-zoster virus. | a sensitive radioimmunoassay for serum antibody to varicella-zoster virus is described; it uses 125i-labeled staphylococcal protein a and a specially designed immunofiltration apparatus. the assay accurately distinguishes between individuals who are susceptible and those who are immune to infection with varicella-zoster virus. in addition, it can detect passive antibody in recipients of varicella-zoster immune globulin. this radioimmunoassay also detects the heterologous antibody responses that ... | 1981 | 6263987 |
| [infections due to herpes-varicella viruses in hodgkin's disease (author's transl)]. | the infections due to herpes-varicella viruses occurring in 191 patients with hodgkin's disease form the basis of this report. there were overall 41 episodes (26.7%) in 40 patients, distributed as follows: varicella in three cases, atypical herpes-varicella in two cases, and herpes zoster in 36 cases, the latter showing systemic spread in seven instances, one to the central nervous system (myelitis) and six to the skin. the mortality was 2.5% of all infections, and 33% of the varicella cases. mo ... | 1981 | 6264237 |
| thygeson's superficial punctate keratitis. | thygeson's superficial keratitis is a chronic disease of the cornea characterized by focal epithelial lesions. we studied 45 cases of thygeson's superficial punctate keratitis. twenty-eight were male and 17 were female patients. the disease had been active in our patients from one month to 24 years. the clinical findings, course, outcome, and response to therapy were studied. viral cultures and microscopic studies were performed on the corneal epithelial scrapings from ten cases. the average cou ... | 1981 | 6264365 |
| recovery of cell-free varicella virus from vero cells. | | 1981 | 6264893 |
| antisera to late cytomegalovirus nuclear antigens produced in cynomolgus monkeys. | | 1981 | 6265560 |
| production of monospecific antibodies to varicella zoster virus antigen in rabbits tolerant to human igg and immunized with antigen immunoprecipitated with human igg from zoster reconvalescent sera. | a method for production of antisera specific to a single antigen of a human infectious agent is described with varicella zoster virus (vzv) as a model. microisolation of immunochemically pure antigen contained in defined immunoprecipitates was achieved by crossed immunoelectrophoresis of a crude antigenic preparation (detergent solubilized, vzv infected cells) into agarose gel containing zoster reconvalescent immunoglobulin. immunization of rabbits made tolerant to human igg with immunoprecipita ... | 1981 | 6265561 |
| varicella-zoster antibodies in patients with hodgkin's disease. | statistically significant differences in mean varicella-zoster antibody titre was found between the normal population and hodgkin's disease group. no difference in mean antibody titre between the four histological categories of the disease was found. | 1980 | 6265816 |
| cytological and immunological examination of cerebrospinal fluid in 9 patients with ramsay hunt's syndrome. | the purpose of the present study was to carry out the immunological and cytological examination of cerebrospinal fluid in 9 patients with ramsay hunt's syndrome. the results obtained were as follows: among the serum immunoglobulins, igg in 1 and igm in 5 of 9 cases increased. the varicella zoster antibody titer was significantly elevated in sera of all 9 cases and in csf of all 7 examined cases. there ws positive fluorescence to varicella zoster virus in 2 (cases 8 and 9) of 4 cases in which csf ... | 1981 | 6267732 |
| screening blood donors for high titre antibody to herpes varicella-zoster. | as a method of increasing supplies of varicella-zoster immunoglobulin, semi-automated screening of blood donors for high titre complement-fixing antibody was initiated. this was compared with the previously used method of donor selection based on a history of varicella or zoster in the past 6 months. about one-third of the "history" donors had titres of greater than or equal to 1 in 64 and generally these levels declined rapidly. in contrast, although only 1.1% of donors in random screening had ... | 1980 | 6267817 |
| viral infections in pediatric burn patients. | bacterial and fungal infections are frequent complications of burns, but the frequency of viral infections has not been defined. in a retrospective survey of serum for viral antibodies in pediatric burn patients, 22% had fourfold increases in antibodies to cytomegalovirus (cmv); 8% had increases to herpes simplex virus and to epstein-barr virus; and 5% had increases to varicella-zoster virus. none of the patients had evidence of adenovirus or hepatitis b virus infection. on the basis of these ob ... | 1981 | 6267934 |
| antibody responses to varicella-zoster virus and the role of antibody in host defense. | antibody titers to varicella-zoster (vz) virus in persons aged 1-85 years were measured. through age 50, the percent of seropostitive individuals continued to rise. there was no fall in geometric mean titer with aging, and there may have been an increase in vz antibody titer in older persons. thus, a fall in vz antibody with increasing age does not seem likely to account for the increased incidence of zoster in the elderly. similarly, immunocompromised patients who are more likely to develop zos ... | 1981 | 6267940 |
| [experiences with the elisa at the serodiagnosis of infections with viruses of the herpes-group (author's transl)]. | a commercially available and standardized enzymeimmunoassay was applied at 737 patients in order to diagnose immunologically a recent or reactivated infection with herpes simplex, herpes zoster v. and/or cytomegalovirus. during this course of investigation rises or falls of the titer of virus specific igg between the range of 1 : 320, 1 : 1280 and greater than or equal to 1 : 5120 turned out to mark mainly recent infections with one of the three herpes viruses. statistical evaluations of the tes ... | 1981 | 6268528 |
| differentiation of members of the human herpesviridae family by radioimmunoassay. | many individuals who are seronegative for one member of the human herpesviridae family are strongly seropositive for other members. using sera from such individuals, the radioimmunoassay technique demonstrated absence of antigen-antibody cross-reactions between varicella-zoster virus (vzv) and herpes simplex virus (hsv) at levels of less than one part in 1,000. sera containing antibody to both hsv and vzv were absorbed with antigens of one agent without significantly altering the amount of remai ... | 1981 | 6268545 |
| inactivation of varicella zoster virus in vitro: effect of leukocytes and specific antibody. | inactivation of varicella zoster virus in vitro by nonadherent, mononuclear peripheral blood leukocytes and antibody is described. when leukocytes and specific antibody were incubated with this virus, marked inactivation of the virus occurred. in contrast, leukocytes alone or with serum devoid of varicella zoster antibody caused only a small degree of inactivation of varicella zoster virus. the leukocytes involved appeared not to be monocyte-macrophages or t or b lymphocytes, and only minute amo ... | 1981 | 6268546 |
| the development and evaluation of a solid-phase radioimmunoassay (ria) procedure for the determination of susceptibility to varicella. | the development of a sensitive solid-phase radioimmunoassay (ria) procedure for the detection of specific antibodies against varicella-zoster (vzv) is described. the test proved to be about 200 times more sensitive than complement fixation (cf) and far more efficient at detecting low levels of antibody in the sera of individuals with past history of varicella. it is suggested that this ria-vzv test would be suitable for distinguishing between individuals who are either immune or susceptible to v ... | 1981 | 6268649 |
| direct immunofluorescence staining for detection of herpes simplex and varicella-zoster virus antigens in vesicular lesions and certain tissue specimens. | direct immunofluorescence (if) staining was compared with virus isolation for detection of herpes simplex viruses (hsv) and varicella-zoster virus (vzv) directly in clinical materials. these included 199 vesicular lesion specimens and 280 tissue specimens. correspondence between if and isolation results was 88% in testing for hsv in lesion specimens and 98% in testing for hsv in various tissue (mostly brain) specimens. overall, if was positive for 82% of the specimens in which hsv was demonstrat ... | 1980 | 6268653 |
| immunization of inbred guinea pigs with varicella-zoster virus grown in a syngeneic transformed embryo cell line. | varicella-zoster virus was serially propagated in a chemically transformed and cloned line of embryo cells (designated "104 c1") derived from the inbred strain 2 guinea pig. when strain 2 guinea pigs were immunized with varicella-zoster virus subcultivated in the syngeneic cell line, they produced high-titer virus-specific antiserum which lacked antibody against cellular determinants. this immunization procedure offers both practical and theoretical advantages over prior methods which involved i ... | 1981 | 6268659 |
| [the elimination of nonspecific reaction in elisa --for the application of routine serological test on varicella zoster, measles and rubella virus (author's transl)]. | | 1981 | 6268717 |
| xbai, psti, and bglii restriction enzyme maps of the two orientations of the varicella-zoster virus genome. | cleavage of varicella-zoster virus dna with the restriction endonucleases psti, xbai, and bglii resulted in 18, 22, and 20 fragments, respectively. based on the molecular weights and molarities of these fragments, a molecular weight of 84 x 10(6) could be calculated for the varicella-zoster virus genome. in both the xbai and the bglii patterns, four 0.5 m fragments were identified. the arrangement of the fragments was determined by molecular hybridization techniques, and the terminal fragments w ... | 1981 | 6268830 |
| vestibular vertigo. a form of polyneuritis? | vestibular vertigo traditionally has been considered to be of viral origin and some forms (acute vestibular neuronitis, acute labyrinthitis, acute vertigo) may be manifestations of a viral cranial polyneuritis. to test this hypothesis, otoneurologic signs and serum complement-fixation titers to herpes simplex and herpes zoster were determined in 16 patients with acute vertigo during their attack and several weeks later. one hundred unselected patients seen for nonotoneurologic problems served as ... | 1981 | 6268853 |
| varicella-zoster virus in the human corneal endothelium: a case report. | | 1980 | 6269677 |
| herpes zoster ophthalmicus in children (reports of two cases). | | 1981 | 6269994 |
| cryopreservation of varicella-zoster virions without loss of structural integrity or infectivity. | varicella-zosterer virions present in infected cells or in a cell-free state were freeze-dried without loss of structural integrity of infectivity. generally, yields of greater than 5 log10 foci/ml (infected cells) or greater than 4 log10 pfu/ml (cell-free virus) were recovered from varicella-zoster virus-infected human melanoma cells both before and after lyophilization in phosphate-buffered media containing 0.1-1.0 m sucrose. virus frozen in solutions lacking sugar had little or no residual in ... | 1981 | 6270035 |
| varicella-zoster virus infection of diploid and chemically transformed guinea-pig embryo cells: factors influencing virus replication. | factors influencing the replication of varicella-zoster virus (vzv) in guinea-pig embryo cells were evaluated using both diploid cells (gpec) and a chemically transformed cell line (gpt). wild-type and vaccine strains of vzv were successfully isolated and serially propagated in gpec prepared from early gestation whole embryos (less than 2 cm in length). low passage gpec (less than or equal to 5 subcultivations) were more susceptible to vzv infection than high passage gpec (greater than 5 subcult ... | 1981 | 6270257 |
| varicella-zoster virus vaccine dna differs from the parental virus dna. | the dnas of a varicella-zoster virus vaccine and its parental virus were compared by cscl buoyant density centrifugation and restriction enzyme cleavage analysis. the varicella-zoster virus vaccine dna showed a heterogeneous buoyant profile and altered restriction enzyme cleavage patterns. these changed properties are probably the result of the accumulation of virus containing defective varicella-zoster virus dna during extensive cell culture passage of the vaccine virus. | 1981 | 6270384 |
| differential affinities of 5-(2-halogenovinyl)-2'-deoxyuridines for deoxythymidine kinases of various origins. | | 1981 | 6270535 |
| the immune system in slow disease due to persistent submicrobial (viral) infection. | | 1981 | 6270625 |
| identification of a primate cytomegalovirus group-common protein antigen. | | 1981 | 6270897 |
| varicella. | | 1981 | 6271007 |
| varicella-zoster virus infection among cancer patients. | | 1981 | 6271032 |
| the plaque-forming factor for mink lung cells present in cytomegalovirus and herpes-zoster virus stocks identified as mycoplasma hyorhinis. | previous investigation of the ability of cytomegalovirus and varicella-zoster virus to replicate in a variety of cell lines suggested that both virus types plaqued with high efficiency in mink lung cells. however, many of the virus isolates used appeared to be contaminated with mycoplasma. we now report that the observed cytopathic effect is due to a mycoplasma which grows lytically to high titre in mink lung cells, but is difficult to cultivate in cell-free media. the mycoplasma was plaque-puri ... | 1981 | 6271903 |
| restriction endonuclease analysis of the dna from varicella-zoster virus: stability of the dna after passage in vitro. | dna was extracted from nucleocapsids isolated from wi-38 cells infected with two different strains of varicella-zoster virus (vzv). the dnas were treated with each of six restriction endonucleases (ecori, hindiii, bg/i, bg/ii, sal i and bam-hi) and small, but reproducible differences in restriction endonuclease patterns were observed. these strains were passaged in wi-38 cells and in primary guinea-pig embryo (gpe) cells, followed by restriction endonuclease analysis of the dnas. no changes were ... | 1981 | 6271904 |
| detection of antibody to varicella-zoster virus by competitive and igm-antibody capture immunoassay. | a simple, sensitive, and specific competitive solid phase immunoassay for antibody to varicella-zoster virus (anti-vzv) is described. the assay uses reagents that can easily be prepared and is sparing of viral antigen. using a solid phase coated with sheep igg from a serum raised against human mu-fc, the same reagents will accurately detect anti-vzv igm. the competitive assay divided sera from children from adults into immune and nonimmune groups that closely correlated with a history of previou ... | 1981 | 6271923 |
| varicella-zoster-associated encephalitis: detection of specific antibody in cerebrospinal fluid. | varicella-zoster (vz) antibody measured by indirect immunofluorescence was used to identify patients with encephalitis due to vz virus. in 15 of 16 (94%) patients with vz infection and clinical evidence of central nervous system involvement, vz antibody was present at a titer of 1:2 or greater in cerebrospinal fluid. in contrast, no vz antibody was detected in the cerebrospinal fluid of seven patients with vz infections without clinical signs of central nervous system involvement, nor was any de ... | 1980 | 6273449 |
| comparison of anticomplement immunofluorescence and fluorescent antibody-to-membrane antigen tests for determination of immunity status to varicella-zoster virus and for serodifferentiation of varicella-zoster and herpes simplex virus infections. | the anticomplement immunofluorescence (acif) test was compared with the fluorescent antibody-to-membrane antigen (fama) test for determining varicella-zoster virus antibody levels as a measure of varicella-zoster virus immunity status. the acif test was found to be comparable to the fama test in sensitivity and could be used for examining sera at low dilutions of 1:2 and 1:4. in addition, the acif method proved to be a more economical procedure in terms of antigen required and personnel time nec ... | 1981 | 6273453 |
| application of a live varicella vaccine in children with acute leukemia or nephrotic syndrome. | | 1981 | 6273490 |
| biochemical transformation of mouse cells by varicella-zoster virus. | mouse l cells lacking the enzyme thymidine kinase (ltk-) were infected with varicella-zoster virus (vzv). even though virus did not replicate in ltk- cells, the presence of virus antigen could be observed by use of an anti-complement immunofluorescent technique at 4 h post-infection and the vzv-specific thymidine kinase could be detected in vzv-infected ltk- cells. ltk-cells were converted to a tk+ phenotype (ltk+) by infection with cell-associated vzv. clones possessing the ability to grow in s ... | 1981 | 6273503 |
| variation on a theme by fenner: the pathogenesis of chickenpox. | | 1981 | 6273782 |
| intrauterine varicella: a report of two cases associated with hyper-a-immunoglobulinemia. | varicella during pregnancy may occasionally harm the fetus. this report described one case of congenital varicella syndrome and another of infantile herpes zoster after maternal varicella in the 16th and 29th week of pregnancy, respectively. a remarkable finding was that both mothers and infants had raised levels of serum iga. this suggests that varicella is prone to lead to congenital disease in infants of pregnant women with an immunologic, perhaps genetically determined abnormality. | 1981 | 6274000 |
| preparation of varicella-herpes zoster immunoglobulin. | the efficacy of zoster immunoglobulin in prophylaxis of varicella and herpes zoster is reported by many authors. the screening for zoster's antibodies is performed in the blood donor population and in persons convalescing from herpes zoster. the results show that 25 per cent of blood donors and 83.5 per cent of convalescents, respectively, have cf antibody titer of l6. varicella zoster immunoglobulin prepared by alcohol fractionation has a fluorescent antibody titer of 1,024 and a cf antibody ti ... | 1981 | 6274067 |
| antibodies in oligoclonal immunoglobulins in csf from patients with acute cerebrovascular disease. | thin-layer polyacrylamide gel isoelectric focusing (pag ief) of csf and serum, and subsequent immunofixation with viral and structural brain components followed by autoradiography revealed in eight out of nine selected patients with oligoclonal csf igg and cerebrovascular disease local synthesis within the cns of antibodies against one or more of the viruses tested: six patients against measles, five against herpes simplex virus type 1, and two against varicella virus. this finding may reflect a ... | 1981 | 6274124 |
| structure of varicella-zoster virus dna. | varicella-zoster virus (vzv) dna was prepared from nucleocapsids and from enveloped virions of a laboratory strain (ellen) and directly from the vesicle fluids of patients with zoster infections. vzv ellen nucleocapsid dna was cleaved with 11 different restriction endonucleases and electrophoresed in agarose gels. the restriction profiles of the nucleocapsid dna were identical to those of the dna recovered from purified virions, but differed from those of another vzv strain (km). in vitro-labele ... | 1981 | 6275100 |