Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| viral reactivation as a cause of unexplained fever in patients with progressive metastatic breast cancer. | patients suffering from metastatic breast cancer and recurrent fever were investigated for viral reactivation or new viral infection as a possible cause of these febrile episodes. three groups of patients were included in the study: (a) patients under adjuvant chemotherapy with cyclophosphamide, methotrexate and fluoruracil, (b) patients with stable metastatic disease treated with cyclophosphamide, fluoruracil and adriamycin or mitoxantrone and (c) patients with progressive metastatic disease wh ... | 1990 | 2159848 |
| [diagnosis of varicella-zoster virus (vzv) infection by using fitc-labeled monoclonal antibodies]. | anti-varicella zoster virus (vzv) mouse monoclonal antibodies conjugated with fluorescein isothiocyanate were evaluated for their usefulness as a practical diagnostic tool in the clinical field by examining cells infected with isolated herpes viruses and 431 clinical samples. the kit stained clearly the cells infected with 14 isolated vzv strains without cross reaction to 15 isolated herpes simplex virus type-1 strains (hsv-1) and 14 type-2 (hsv-2) strains. in clinical specimens, viral antigens ... | 1990 | 2159967 |
| [a clinical and pathological study on varicella-zoster virus pneumonia]. | a 48-year-old male with adult t-cell leukemia (case 1) and a 57-year-old with acute lymphocytic leukemia (case 2) died of rapid progressive pneumonia and pleuritis. histopathological findings of the lungs were multifocal hemorrhagic coagulation necrosis, and typical cowdry type a and full type intranuclear inclusion bodies were observed in alveolar cells, bronchial cells, fibroblast, endothelial cells of small vessels, bronchial gland cells and pleural cells. antigen against varicella-zoster vir ... | 1990 | 2159968 |
| [varicella vaccination]. | basing of a review of the world literature the problems of the live attenuated varicella vaccine are presented. the vaccine is well tolerated and immunogenic. side effects are unimportant. the vaccination is associated with a decrease of the chickenpox attack rate following an intensive exposure to varicella-zoster virus conferring about 80% protection in immunocompromised children over a period of 3-5 years. the vaccination provide an excellent protection against severe varicella in children wi ... | 1990 | 2160635 |
| viral pneumonia in recipients of solid organ transplants. | viral pulmonary infections are a major cause of morbidity and mortality in solid organ transplant recipients. the herpes viruses-cytomegalovirus, herpes simplex virus, varicella zoster virus, and epstein-barr virus--cause most of the viral infections in this population. respiratory syncytial virus, adenovirus, and human immunodeficiency virus also cause pneumonitis in the transplant recipient. differences in the clinical and laboratory presentation of pneumonitis due to the various viral agents ... | 1990 | 2160718 |
| severity of viremia and clinical findings in children with varicella. | the number of varicella zoster virus-infected mononuclear cells in blood samples obtained during 3 days before appearance of vesicular skin rashes could be estimated in 13 of 21 immunocompetent children with varicella and viremia (range, 1-83 per 10(7) mononuclear cells). the virus was isolated from 5% of adherent cells (1/21), 71% of nonadherent cells (12/17), 43% of t lymphocytes (9/21), and 33% of b lymphocytes (7/21). the number of vesicles on the body correlated with the maximum body temper ... | 1990 | 2161037 |
| viral vaccines of the future. | varicella vaccine seems close to licensure in the united states. it is likely to be recommended for routine use in healthy children, either administered singly or as a combination of mmr. healthy children who have been immunized develop excellent antibody and cell-mediated responses to vzv in the absence of significant adverse effects, and they are well protected against subsequent infection with vzv. although it will not be known for certain for many years, it seems most unlikely that immunizat ... | 1990 | 2161508 |
| detection of specific iga antibodies to varicella zoster virus in serum of patients with ramsay hunt syndrome. | varicella zoster virus (vzv)-specific igg and iga antibody titers were determined in serial serum samples of 23 patients with ramsay hunt syndrome by the immunoperoxidase assay. varicella zoster virus-specific igg antibodies were found in the first serum samples of all the patients. in 80% of 20 patients in whom a serum sample was available within 5 days after the onset of the disease. vzv-specific iga antibodies were detected. the second serum sample was vzv-specific iga-positive in all of the ... | 1990 | 2161635 |
| murine herpesvirus 68 is genetically related to the gammaherpesviruses epstein-barr virus and herpesvirus saimiri. | short nucleotide sequence analysis of seven restriction fragments of murine herpesvirus 68 (mhv-68) dna has been undertaken and used to determine the overall genome organization and relatedness of this virus to other well characterized representatives of the alpha-, beta- and gammaherpesvirus subgroups. nine genes have been identified which encode amino acid sequences with greater similarity to proteins of the gammaherpesvirus epstein-barr virus (ebv) than to the homologous products of the alpha ... | 1990 | 2161903 |
| two binding sites for the herpes simplex virus type 1 ul9 protein are required for efficient activity of the oris replication origin. | two sites within the short region origin of dna replication (oris) in herpes simplex virus type 1 (hsv-1) which bind the product of the ul9 gene have previously been identified. one of these sites (site i) contains an 11 bp sequence which is also present in oris of varicella-zoster virus, and the other (site ii) includes a related element differing in two positions. a third sequence (motif iii), which lies close to binding site i, differs from the site i element at only a single position. we hav ... | 1990 | 2161905 |
| a dot-elisa for the detection of igg antibodies to mumps and varicella viruses. | an enzyme-linked immunosorbent assay using nitrocellulose strips (dot-elisa) for the routine laboratory detection of igg antibodies to mumps and varicella viruses is described. the virus antigens are dotted onto nitrocellulose strips, and the dotted strips are incubated with the sera to be tested. the bound antibodies are revealed using enzyme-labeled antihuman igg antibodies. reliable results are obtained when the assay is carried out at 37 degrees c. the reported data indicate that the dot-eli ... | 1990 | 2161916 |
| detection of varicella-zoster virus dna by field-inversion gel electrophoresis. | a new method for detection of varicella-zoster virus (vzv) dna using field-inversion gel electrophoresis (fige) was devised. vzv-genomic dna could be differentiated from the host cell dna of human embryonic lung (hel) fibroblasts infected with vzv under electrophoretic conditions allowing resolution of linear and double-stranded dnas in the 49-230 kilobase pairs (kb) range. the detection of vzv-genomic dna from infected hel cells was successful regardless of whether the vzv was a laboratory stra ... | 1990 | 2161996 |
| new acquisitions in the chemotherapy of viral infections. | the development of new antiviral agents has gained increasing momentum. it has kept pace with the identification of specific sites ("targets") in the virus replicative cycle at which potential antiviral drug can interact. the current armamentarium of available antiviral drugs consists of amantadine and rimantadine (against influenza a), ribavirin (against respiratory syncytial virus infection), idoxuridine and trifluridine (against herpetic keratitis), vidarabine and acyclovir (against herpes si ... | 1990 | 2162118 |
| [serodiagnosis of varicella zoster virus infection]. | 1990 | 2162422 | |
| viral infections associated with bone marrow transplantation. | bone marrow transplantation is complicated by a sequential occurrence of viral infections, the predictability of which influences disease management. among these infections are herpes simplex virus, cytomegalovirus, varicella zoster virus, epstein-barr virus, respiratory viral infections, hepatitis viral infections, and gastrointestinal infections. the approach to the treatment and prevention of these infections is discussed. | 1990 | 2162815 |
| acute and persistent varicella-zoster virus infection of human and murine neuroblastoma cell lines. | human and murine neuroblastoma cell lines were infected in vitro with varicella-zoster virus (vzv). infected human neuroblastoma cells (imr-32) supported the synthesis of abundant viral antigens as detected by indirect immunoperoxidase labeling using human serum rich in anti-vzv antibodies and did not survive the infection. in situ hybridization (ish) with vzv-cloned probes revealed a strong hybridization signal in these infected cells. during cultivation, the virus was released in the culture m ... | 1990 | 2162972 |
| a new method of detecting hyperimmune anti-varicella zoster virus. | 1990 | 2163128 | |
| fine specificities of antibodies in sera and cerebrospinal fluid in herpes virus infections of the central nervous system as detected by the antigen variable immunoblot technique. | applying the immunoblot technique a sensitive and specific method was developed for the detection of intrathecally synthesized antibodies against individual specific proteins that are antigens of various infectious agents causing encephalitis. paired serum and cerebrospinal fluid (csf) samples from five patients with herpes virus infections of the central nervous system (cns) (three herpes simplex virus encephalitis, one varicella zoster virus encephalitis, one zoster ganglionitis) were investig ... | 1990 | 2163408 |
| drug susceptibilities of isolates of varicella-zoster virus in a clinical study of oral brovavir. | susceptibilities to brovavir [1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)-uracil] and acyclovir of clinical isolates of varicella-zoster virus obtained from 58 patients with herpes zoster, included in a clinical trial of oral brovavir, were tested by a plaque reduction method. all 101 isolates were significantly susceptible to brovavir; 50% effective dose of brovavir for these isolates ranged between 0.6-4.0 ng/ml (average: 1.29 ng/ml). brovavir was about 3,000 times more potent than acyclovir ... | 1990 | 2163486 |
| identification of the cross-reactive antigens between marek's disease virus and pseudorabies virus by monoclonal antibodies. | among the 33 monoclonal antibodies (mabs) against pseudorabies virus (prv) examined, three mabs (24-17, 74-26, and 8) were found to react with cells infected with marek's disease virus (mdv)-related viruses by immunofluorescence test. two of the mabs (24-17 and 74-26) reacted with the nuclei of cells infected with mdv serotype 1 (mdv1), mdv serotype 2 (mdv2), and herpesvirus of turkeys (hvt), whereas mab 8 reacted with the cytoplasm of mdv2- and hvt-infected cells. however, none of the mabs agai ... | 1990 | 2164393 |
| in situ hybridisation in herpetic lesions using a biotinylated dna probe. | in situ hybridisation was performed with a biotinylated dna probe for herpes simplex virus (hsv) using high temperature denaturation on formalin fixed, paraffin wax sections of lung, brain, ganglion and keratinising and non-keratinising squamous epithelia. eosinophilic viral nuclear inclusions or characteristically moulded multiple nuclei with altered chromatin, which were present in two cases of hsv encephalitis and one case of viral pneumonitis, all showed complete hybridisation visualised by ... | 1990 | 2164533 |
| analysis of the binding sites for the varicella-zoster virus gene 51 product within the viral origin of dna replication. | the c-terminal 322 amino acids of the varicella-zoster virus (vzv) gene 51 product were expressed in escherichia coli and shown to bind to specific dna sequences within the vzv origin of dna replication. the gene 51 product and its herpes simplex virus type 1 homolog (the ul9 protein) are capable of recognizing identical dna sequences but the arrangement of binding sites within the origin regions of the two viruses differs. three binding sites for the vzv gene 51 protein were identified within t ... | 1990 | 2164726 |
| effect of influenza virus strains on lipid metabolism of infected hek cells. | we have shown previously that in infected hel cells varicella-zoster virus (vzv) causes a shift from polar to neutral lipid synthesis and that some strains of the virus depressed total lipid synthesis. in this report we show that vzv produces a similar effect on the lipid metabolism of infected human embryonic kidney cells. the pattern of lipid synthesis in human embryonic kidney cells infected with either of two strains of influenza type a virus was similar to that of control uninfected cells, ... | 1990 | 2165041 |
| strategies for the management of varicella-susceptible healthcare workers after a known exposure. | three different sequentially applied post-varicella zoster virus (vzv) exposure management strategies were employed over a 43-month period. we began by using a standard post-exposure protocol in which 50 susceptible healthcare workers (hcw) involved in hospital exposures were furloughed from work at a loss to the hospital of 424 workdays and $46,000. of the eight nosocomial cases of vzv infection in hcws, four (50%) caused future hcw and patient exposure. in trial i, we substituted a post-exposu ... | 1990 | 2165080 |
| hsv-1 gb and vzv gp-ii crossreactive antibodies in human sera. | the specificity and prevalence of human igg antibodies crossreactive between hsv-1 (ang) and vzv (ellen) was examined in immunoblots. using antibody fractions purified on hsv- and vzv-coated affinity chromatography columns and by preadsorption of sera with hsv and/or vzv lysates a crossreactivity between hsv-1 gb and vzv gp-ii was demonstrated. crossreaction of human igg antibodies among other structural and nonstructural viral proteins, however, was not detected. the frequency of human igg anti ... | 1990 | 2165766 |
| other common types of viral hepatitis and exotic infections. | this chapter discusses two distinct sets of viruses which cause hepatitis. the first include common virus infections which cause mild hepatitis rarely in individuals with normal immunity but which may cause severe life-threatening hepatitis in the increasing number of patients with depressed cell mediated immune responses. the second comprise exotic virus infections which are prevalent in particular geographical areas and which frequently involve the liver with high mortality. for each of these ... | 1990 | 2165845 |
| microplate hybridization of amplified viral dna segment. | we have developed a simple hybridization method for a dna segment which is amplified by the polymerase chain reaction: after heat denaturation, the amplified dna segment with a length of more than 300 bases is adsorbed to microplate wells in the presence of 1.5 m nacl or 0.5 m ammonium sulfate; the immobilized dna is hybridized with a biotin-labeled dna probe; then, the hybridization signal is detected by streptavidin-conjugated beta-galactosidase or peroxidase. this method has several advantage ... | 1990 | 2166086 |
| unusual varicella zoster virus infection in a patient with colon carcinoma and evans syndrome--delayed virus shedding generalized recurrent necrotic herpes zoster. | a 53-year-old japanese woman with evans syndrome and colon cancer had two episodes of herpes zoster. the first painful vesicular rashes involved the right lower abdomen and buttock and healed in one month. after one more month, a second attack occurred on the right thigh and leg and developed into generalized hemorrhagic lesions, which became crusted in about 90 days. the patient died 131 days after the second attack, when the lesions had almost subsided. varicella-zoster virus (vzv) was isolate ... | 1990 | 2166097 |
| varicella in patients infected with the human immunodeficiency virus. | in a retrospective study of 421 patients infected with human immunodeficiency virus, 15 (3.5%) had varicella. twelve patients had a typical varicella. complications were as follows: profuse eruption, 6; hemorrhagic eruption, 1; hepatitis, 5; and pulmonary involvement, 1; 1 patient developed an intravascular disseminated coagulation and died of varicella. three patients with acquired immunodeficiency syndrome, having a history of varicella, presented with an atypical form of varicella with a smal ... | 1990 | 2166482 |
| prolonged cutaneous herpes zoster in acquired immunodeficiency syndrome. | we described the development of prolonged disseminated cutaneous herpes zoster in two patients with acquired immunodeficiency syndrome. both patients developed hyperkeratotic, verrucous lesions that progressed despite acyclovir therapy. the biopsy specimens were typical of herpes infection. the development of acyclovir-resistant varicella-zoster virus during therapy was suspected clinically in the first patient and documented in vitro in the second patient. the inability to mount an effective ce ... | 1990 | 2166483 |
| detection of locally produced antibodies to herpes viruses in the aqueous of patients with acquired immune deficiency syndrome (aids) or acute retinal necrosis syndrome (arn). | intraocular synthesis of igg antibodies against hsv (herpes simplex virus), cmv (cytomegalovirus) and vzv (varicella zoster virus) is considered as an indirect proof of uveoretinal infection. paired serum and aqueous samples obtained from 16 patients with retinitis associated with aids, 3 patients with arn, 8 patients with posterior uveitis not related to aids or arn and 5 patients with senile cataract were tested for total immunoglobulin g levels and antibodies to hsv, cmv and vzv by the fixed ... | 1990 | 2166640 |
| the glycoprotein products of varicella-zoster virus gene 14 and their defective accumulation in a vaccine strain (oka). | many characteristics of the putative protein encoded by varicella-zoster virus (vzv) open reading fram (orf) 14 indicate that it is a glycoprotein, which has been designated gpv. to identify the protein products of the gene, the coding sequences were placed under the control of the vaccinia virus p7.5 promoter and recombinant vaccinia viruses were constructed. heterogeneous polypeptides with molecular weights of 95,000 to 105,000 (95k to 105k polypeptides) were expressed in cells infected by a v ... | 1990 | 2166829 |
| latent varicella-zoster viral dna in human trigeminal and thoracic ganglia. | some human herpesviruses become latent in dorsal-root ganglia. primary infection with the varicella-zoster virus causes chickenpox, followed by latency, and subsequent reactivation leading to shingles (zoster), but the frequency and distribution of latent virus have not been established. | 1990 | 2166914 |
| [acyclovir and specific anti-varicella-herpes zoster immunoglobulins in the treatment of varicella-zoster virus infection in 113 adults]. | from january 1978 to december 1988, 54 males and 59 females were treated for varicella, zoster and disseminated zoster by varicella-zoster immunoglobulin (group i) or acyclovir (group ii). 67 patients had immune deficiency disease. treatment was successful for 92/100 patients in group i and for 100/100 patients in group ii. thrombophlebitis and renal failure were observed in group ii and regressed when acyclovir was stopped. varicella-zoster immunoglobulin and acyclovir are two effective therape ... | 1990 | 2166935 |
| monoclonal antibody to immediate early protein encoded by varicella-zoster virus gene 62. | monoclonal antibodies (mabs) were prepared against varicella-zoster virus (vzv)-infected cell proteins, and 10 mabs which reacted with nuclear antigens were selected. these mabs recognized a major 175-180 kda and three minor vzv-specific phosphoprotein species. immunofluorescence staining of vzv-infected cells showed that the 175-180 kda protein was synthesized within 6 h after infection. the synthesis of this protein was inhibited by cycloheximide (ch); however, reversal of ch treatment and add ... | 1990 | 2166981 |
| emergence of acyclovir-resistant varicella zoster virus in an aids patient on prolonged acyclovir therapy. | we demonstrate for the first time the appearance of acyclovir resistance in serial varicella zoster isolates from a patient treated with acyclovir. we recovered varicella zoster virus three times over a period of 5 months from the skin lesions of this patient with aids who was treated with three courses of intravenous acyclovir and prolonged low-dose oral acyclovir. the isolate recovered from a typical zoster lesion before acyclovir, and one obtained from a hyperkeratotic lesion 2 months later, ... | 1990 | 2167103 |
| [a case of unilateral viiith, ixth and xth cranial nerve involvement with herpes zoster]. | a 46-year-old healthy man suffered from sore throat, fever and right otalgia. on the next day, he developed hoarseness and difficulty in swallowing. on the 6th day, he suffered from vertigo, nausea and vomiting associated with unsteady gait. he was admitted to the otorhinolaryngology department in our hospital and pointed out to have vesicles at his right ear. on the 13th day, he was referred to our service. on admission, no vesicles were noted at the right ear or pharynx. neurological examinati ... | 1990 | 2167188 |
| subclass distribution of the serum and intrathecal igg antibody response in varicella-zoster virus infections. | the subclass distribution of the varicella-zoster virus (vzv)-specific igg antibody response was studied in serum samples from 22 patients with primary varicella and 34 with recurrent vzv infections and in cerebrospinal fluid (csf) samples from 22 patients with recurrent infection who presented with symptoms of aseptic meningitis. igg1 and 3 were the dominant subclasses among patients with primary and recurrent infections; igg1 was also prevalent in the csf samples. the vzv igg subclass distribu ... | 1990 | 2167334 |
| antibodies to varicella-zoster virus glycoproteins i, ii, and iii in leukemic and healthy children. | a dot elisa was used to analyze the antibody response to varicella-zoster virus glycoproteins i, ii, and iii in leukemic and healthy children who either developed natural varicella or received the live attenuated varicella vaccine. both groups of children showed an antibody response to these viral glycoproteins. the antibody response to all three glycoproteins showed excellent persistence over the 3-year period of study. none of the glycoproteins provoked an antibody response that could be shown ... | 1990 | 2167335 |
| a rapid and simplified micromethod for subtyping varicella-zoster virus. | a simplified and rapid micromethod based on restriction endonuclease analysis of radiolabelled varicella-zoster virus (vzv) dna is described and applied to strains for comparison. this procedure is cheaper and less time consuming than that requiring viral nucleocapsid purification (macromethod). the micromethod is suitable for routine dna analysis of vzv isolates, allows differentiation of vaccine strain from wild strains, and provides evidence for variability of wild strains. | 1990 | 2167348 |
| cell surface expression of the varicella-zoster virus glycoproteins and fc receptor. | varicella-zoster virus (vzv) specifies the synthesis of viral glycoproteins which are important antigens for induction of the host immune response. in this report the technology of laser-activated flow cytometry has been employed to measure the membrane expression of vzv glycoproteins gpi, gpii, gpiii, and gpiv. by use of biotinylated monoclonal antibodies as probes, all four glycoproteins were demonstrated on the infected cell surface. the temporal appearance of the viral glycoproteins was defi ... | 1990 | 2167554 |
| identification of two genes in the unique short region of pseudorabies virus; comparison with herpes simplex virus and varicella-zoster virus. | we have determined the nucleotide sequence of two genes in the unique short region of the genome of pseudorabies virus (prv). near the internal repeat, upstream of the gene encoding glycoprotein gx, we identified an open reading frame (orf) encoding a protein of 390 amino acids. we designated this gene pk because the predicted protein contains most of the conserved motifs of a eukaryotic protein kinase. the protein shares amino acid homology with the protein kinases encoded by gene us3 of herpes ... | 1990 | 2167928 |
| the nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein h gene. | the equine herpesvirus 4 (ehv-4) gene glycoprotein h (gh) gene homologue was localized by virtue of the conserved genomic position of this gene throughout members of the herpesvirus family. the gene maps immediately downstream of the thymidine kinase gene at approximately 0.49 to 0.51 map units within genomic fragment bamh1 c. the ehv-4 gh primary translation product is predicted to be a polypeptide of mr 94,100, 855 amino acids long, which possesses features characteristic of a membrane glycopr ... | 1990 | 2167933 |
| synthesis and antiviral activity of some 3'-c-difluoromethyl and 3'-deoxy-3'-c-fluoromethyl nucleosides. | the synthesis and antiviral activity of a number of 3'-c-difluoromethyl and 3'-deoxy-3'-c-fluoromethyl nucleosides are reported. the 3'-c-difluoromethyl nucleosides 26a and 26b were obtained by treatment of the corresponding 2',5'-di-o-protected-3'-c-formyl nucleosides 25a and 25b with (diethylamino)sulfur trifluoride (dast). removal of the 2'-o-protecting group from 26a and subsequent reaction with dast furnished the 2'-deoxy-2'-fluoro-beta-d-ribo-pentofuranosyl nucleoside 29. selective fluorin ... | 1990 | 2167980 |
| synthesis and antiviral activity of 3'-deoxy-3'-c-hydroxymethyl nucleosides. | a series of 3'-branched-chain sugar nucleosides, in particular 3'-deoxy-3'-c-hydroxmethyl nucleosides, have been synthesized and evaluated as antiviral agents. reaction of 1-(2,3-epoxy-5-o-trityl-beta-d-lyxo-pentofuranosyl) derivatives 12 and 13, of uracil and thymine, respectively, with 5,6-dihydro-2-lithio-5-methyl-1,3,5-dithiazine 14 afforded the corresponding 3'-functionalized nucleosides 15 and 16, respectively. replacement of the trityl group with tertbutyldiphenylsilyl allowed high yieldi ... | 1990 | 2167981 |
| high incidence of herpes zoster in patients with systemic lupus erythematosus: an immunological analysis. | the incidence of herpes zoster was determined in patients with systemic lupus erythematosus (sle) and the cellular and humoral immunity to varicella zoster virus (vzv) investigated in 45 of these 92 patients. the incidence of herpes zoster was high, occurring in 40 patients (43%), though it was benign in all. patients with sle who had had zoster showed significantly higher antibody titres than normal subjects. on the other hand, only 13 of 43 (30%) patients with sle showed positive delayed hyper ... | 1990 | 2168693 |
| cerebral vasculopathy associated with primary varicella infection. | a previously healthy 5-year-old boy developed cerebral vasculopathy, presenting as two episodes of acute hemiparesis 3 and 9 months, respectively, after a primary varicella infection (chickenpox). this association has not been reported before, to our knowledge, although cerebral vasculopathy is a well-known complication of herpes zoster ophthalmicus. the diagnosis was based on the presence of oligoclonal varicella-specific igg in the cerebrospinal fluid and angiographic findings. clinical and an ... | 1990 | 2168697 |
| [varicella hepatitis]. | 1990 | 2169050 | |
| [intrathecal secretion of antiviral antibodies in multiple sclerosis in patients in seville]. | to assess the presence of antiviral antibody synthesis in the cerebrospinal fluid in patients with multiple sclerosis, concurrent plasma and spinal fluid determination of antibody titers against measles, varicella-zoster, rubella, mumps, cytomegalovirus, and herpes viruses were performed in 29 samples and were compared with a control group. the study revealed an increased titre of antiviral antibodies in the spinal fluid in patients with multiple sclerosis. this increased activity was markedly s ... | 1990 | 2169273 |
| cell-mediated immune responses after immunization of healthy seronegative children with varicella vaccine: kinetics and specificity. | humoral and cell-mediated immune responses were determined in seronegative children immunized with live attenuated oka strain varicella vaccine. at 2 weeks after immunization, 80% of children had detectable lymphocyte proliferation to varicella-zoster virus (vzv) antigens, while only 40% had antibodies to vzv as detected by elisa. by 6 weeks after immunization, 97% of children seroconverted, and 95% of these responded to vzv antigens in the proliferation assay. a high proportion of immunized chi ... | 1990 | 2169495 |
| endemic kaposi's sarcoma in human immunodeficiency virus type 1-seronegative persons: demonstration of retrovirus-like particles in cutaneous lesions. | in 1984, greek physicians reported on the clustering of cases of kaposi's sarcoma (ks) on the peloponnesus peninsula. to gain more insight into its pathogenesis, we studied the seroepidemiologic and clinicopathologic characteristics of 12 greek ks patients (eight male/four female) five of whom were residents of an endemic area on the peloponnesus. these patients were in good general health with ages ranging from 48 to 80 years, had no clinical signs of immunodeficiency, and combined the features ... | 1990 | 2170537 |
| induction of cervical neoplasia in the mouse by an extract of cells infected by varicella-zoster virus. | since several human herpesviruses, including varicella-zoster virus (vzv), have been demonstrated to transform mammalian cells in vitro, vzv was tested in a mouse model of virus-induced cervical neoplasia to determine whether it is oncogenic in vivo. herpes simplex viruses types 1 and 2 and cytomegalovirus have been previously shown to induce cervical neoplasia in this mouse model. vzv was propagated in wi-38 cell cultures and inactivated by ultraviolet irradiation. control material was prepared ... | 1990 | 2170577 |
| [detection of herpes simplex virus, varicella zoster virus and cytomegalovirus in aphthous stomatitis]. | attempts were made to demonstrate herpes simplex virus (hsv) type 1 and type 2, varicella zoster virus (vzv) and cytomegalovirus (cmv) in specimens obtained from aphthous ulceration lesions by the immunofluorescent method using fluorescein-labeled monoclonal antibodies. hsv-1 and vzv were detected in 2 and 4 out of 30 patients, respectively. although almost all viruses that can infect the oral cavity could occasionally cause stomatitis, neither hsv-2 nor cmv was not found in this study. vzv was ... | 1990 | 2170607 |
| [herpesvirus latency]. | the article reviews present knowledge about the molecular mechanisms involved in herpes virus latency. the work on herpes virus latency has created a foundation for much of our knowledge about viral latency in general. herpes simplex virus is perhaps best known in this respect. this virus resides latently in sensory ganglia. no herpes simplex-specific proteins have been found, but a specific rna transcript has been identified. in varicella-zoster virus latency several latency-associated transcri ... | 1990 | 2171156 |
| the nucleotide sequence of the equine herpesvirus 4 gc gene homologue. | the genomic position of an equine herpesvirus 4 (ehv-4) gene homologue of the herpes simplex virus 1 (hsv-1) gc gene was determined by southern analysis and dna sequencing. the gene lies within a 2-kbp bg/ii-ecori fragment mapping between 0.15 and 0.17 within the long unique component of the ehv-4 genome and is transcribed from right to left. putative promoter elements were identified upstream of the 1455-bp open reading frame which encodes a 485-amino-acid protein of unglycosylated molecular we ... | 1990 | 2171212 |
| molecular biology of varicella zoster virus. | 1990 | 2171303 | |
| central nervous system lymphoma in the acquired immunodeficiency syndrome. | primary central nervous system (cns) lymphomas were studied in fifteen autopsied patients with the acquired immunodeficiency syndrome (aids). using the working formulation for non-hodgkin's lymphomas, the tumors were classified as large cell (7 patients), mixed large and small cell (6 patients), small cleaved cell (1 patient), and unclassifiable (1 patient). the mixed lymphomas displayed unusual features characterized by a high mitotic rate and the presence of numerous medium-sized cells (5 to 1 ... | 1990 | 2171824 |
| acyclovir treatment for varicella does not lower gpi and ie-62 (p170) antibody responses to varicella-zoster virus in normal children. | the varicella-zoster virus (vzv) membrane glycoprotein gpi elicits a major immunoglobulin g antibody response after naturally acquired vzv infection; antibody to a nonglycosylated immediate-early protein, ie-62 (p170), represents a response to a nonmembrane vzv component. we evaluated antibody response to vzv gpi and ie-62 (p170) at 28 days and 1 year following infection in 34 children (ages 5 to 16 years) enrolled in a randomized placebo-controlled study of oral acyclovir for the treatment of v ... | 1990 | 2172288 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| the immunogenicity of the oka/merck varicella vaccine in relation to infectious varicella-zoster virus and relative viral antigen content. | humoral and cellular immune responses to whole varicella-zoster virus (vzv) antigen and to the vzv glycoprotein i (gpi) and immediate early protein (ie-62) were compared in two populations of healthy children who received different lots of the oka/merck varicella vaccine. children who were given vaccine containing 950 pfu with a relative antigen content of 1.0 (lot c-k472) per dose, in an earlier protocol, had an initial seroconversion rate of 87% but vzv cell-mediated immunity was diminished at ... | 1990 | 2172393 |
| evolutionary relationships of virion glycoprotein genes in the s regions of alphaherpesvirus genomes. | the short region in the genome of herpes simplex virus type 1 contains a contiguous array of five genes (us4, us5, us6, us7 and us8) which encode known or proposed virion-surface glycoprotein species. counterparts for certain of these have been described in the genomes of other alphaherpesviruses, namely herpes simplex virus type 2, pseudorabies virus and varicella-zoster virus. within each of the us4-, us6- and us7-related sets, the amino acid sequences are most conserved in a region containing ... | 1990 | 2172448 |
| nucleotide sequence of a cytomegalovirus single-stranded dna-binding protein gene: comparison with alpha- and gammaherpesvirus counterparts reveals conserved segments. | the genomic sequence encoding a cytomegalovirus strain colburn homologue (db129) of the herpes simplex virus major dna-binding protein (icp8) was determined. multiple alignments of the deduced db129 amino acid sequence and three alpha- and gammaherpesvirus homologues revealed that 56% of the amino acid residues identical in all four homologues are contained within 12 relatively conserved segments, which together constitute only 11.2% of the shortest aligned sequence. in light of published icp8 d ... | 1990 | 2172458 |
| antibodies to viral antigens, xenoantigens, and autoantigens in alzheimer's disease. | sera from 19 patients with alzheimer's disease (ad) and 21 control subjects were studied by immunofluorescence and enzyme immunoassay for antibody activity against various viruses and 12 self- and non-self-antigens. total igg mean level was significantly higher in the ad group; the igg level was above 15 g/l in 52.8% of ad patients versus 14.3% of control subjects. antiviral antibody titers showed no significant differences except for antibodies to herpes simplex virus-1, which were increased in ... | 1990 | 2172499 |
| [a complicated varicella infection]. | 1990 | 2172844 | |
| studies on the pathogenesis of neurological diseases associated with varicella-zoster virus. | immunocytochemical techniques and in situ hybridization with three different varicella-zoster virus (vzv)-specific rna probes have been used to study the pathogenesis of vzv-associated neurological syndromes. varicella-zoster virus antigens were not detected using the avidin-biotin peroxidase technique with a polyvalent anti-vzv antibody in any of the formalin-fixed tissue sections from eight cases of vzv-associated neurological disease (encephalitis, myelitis, ganglionitis); one case was immuno ... | 1990 | 2172854 |
| varicella-zoster virus infection of human astrocytes, schwann cells, and neurons. | human fetal cell cultures enriched for astrocytes, schwann cells, or dorsal root ganglia neurons were infected with cell-free varicella-zoster virus (vzv), and the course of these infections was compared with that in fetal fibroblasts. virus replication was detected in each neural cell type as early as 10-16 hr postinfection. permissiveness of each cell type was confirmed by electron microscopy. however, the kinetics of virus spread varied between the neural cell types. moreover, the accumulatio ... | 1990 | 2173263 |
| adenovirus infection in patients with kawasaki disease. | two outbreaks of kawasaki disease at different times and areas (kyoto in 1982 and sapporo in 1985) of japan were studied retrospectively for the presence of antibodies to adenoviruses and herpesviruses. only 2 of 12 (16.7%) consecutive acute phase sera of patients from the outbreak in 1982 and 1 of 10 (10.0%) sera from 1985 showed positive antibodies for the common adenovirus antigen by a complement fixation (cf) test, whereas 10 of 16 (62.5%) age- and sex-matched controls during the outbreak of ... | 1990 | 2173738 |
| the possible role of herpes viruses in multifocal choroiditis and panuveitis. | 7 cases of multifocal choroiditis and panuveitis are reported here (6 females, 1 male). all clinical data were carefully considered. in all cases an aqueous sampling was made for the detection of anti-herpes virus antibodies in aqueous and serum. 3 specificities were tested: herpes simplex (hsv), herpes zoster (hvz) and cytomegalovirus (cmv). an intraocular synthesis of specific antibodies was found against vzv in 2 cases and against hsv in 1 case. there was another presumptive case for hsv. | 1990 | 2174419 |
| [acute retinal necrosis--a herpetic retinitis?]. | acute retinal necrosis (arn) is characterized by unilateral or bilateral severe vaso-occlusive retinitis. in a large number of cases the patients become blind in the affected eyes as a direct result of the acute inflammation or the subsequent retinal detachment. the etiology is poorly understood; herpesvirus-induced vasculitis is presumed and has been demonstrated by some authors. however, the author's own investigations in seven patients show that even vitreous biopsy reveals a herpetic origin ... | 1990 | 2174421 |
| glycoproteins encoded by varicella-zoster virus: biosynthesis, phosphorylation, and intracellular trafficking. | this article reviews major developments concerning the vzv glycoproteins. very little was published about these viral products prior to the early 1980s. vzv is now known to encode five glycoproteins that have been designated gpi through gpv. the viral glycoproteins are present within the envelope of the complete virion; in addition, they are inserted in the plasma membrane of the infected cell. studies of the assembly and processing of three of the vzv glycoproteins have demonstrated that they m ... | 1990 | 2174668 |
| postsurgical zosteriform herpes simplex 2 in noncontiguous dermatomes. | 1990 | 2174931 | |
| a herpes simplex virus type 1 recombinant with both copies of the vmw175 coding sequences replaced by the homologous varicella-zoster virus open reading frame. | varicella-zoster virus (vzv) gene 62 encodes a protein with a predicted mr of 140,000 (vzv 140k) that shares considerable amino acid homology with the immediate early (ie) regulatory protein vmw175 of herpes simplex virus type 1 (hsv-1) and is believed to be its functional equivalent. we have tested this hypothesis by insertion of vzv gene 62 (expressed from the hsv-1 ie3 promoter) into both ie3 gene loci in the short region repeats of the hsv-1 genome. the parent virus used for this manipulatio ... | 1990 | 2174959 |
| early diagnosis of enteroviral meningitis by a solid-phase reverse immunosorbent test and virus isolation. | 45 cases of aseptic meningitis/meningoencephalitis were studied with regard to enteroviral etiology by virus isolation and solid-phase reverse immunosorbent test (sprist), a cross-reacting test for enterovirus igm. an etiological diagnosis was reached in 37/45 (82%) patients. etiological diagnoses other than enteroviruses were found in 8 patients: borrelia burgdorferi in 4, varicella-zoster virus in 2, herpes simplex virus in 1 and mumps virus in 1 patient. enteroviruses (echovirus 6, 21 and 30) ... | 1990 | 2175448 |
| virus detection in the fetal tissue of a premature delivery with a congenital varicella syndrome. a case report. | cases of congenital varicella syndrome have been published, to date, a single case reports. isolation attempts of varicella-zoster virus from fetal tissues have, thus far, been unsuccessful. this is a first report of detection of varicella-zoster virus in fetal tissue by means of dna hybridization technique in a typical case of congenital varicella syndrome in a premature delivery of the 27th gestational week. the case is documented anamnestically, sonographically, pathologically and virological ... | 1990 | 2175786 |
| a.i.d.s. patients. variable test results. | 1990 | 2176278 | |
| equine herpesvirus type 1 unique short fragment encodes glycoproteins with homology to herpes simplex virus type 1 gd, gi and ge. | the nucleotide sequence of a 6.4 kbp portion of the 10.6 kbp bamhi fragment d contained in the unique short region of the equine herpesvirus type 1 (ehv-1) genome has been determined. analysis of this sequence revealed five open reading frames (orfs), four complete and one incomplete, which were encoded by the same sense strand. comparison of the ehv-1 dna sequence with that encoding glycoproteins of other alphaherpesviruses has revealed no significant homologies. comparison at the amino acid le ... | 1990 | 2177089 |
| the product of varicella-zoster virus gene 62 autoregulates its own promoter. | varicella-zoster (vzv) gene 62 encodes a protein with a predicted mr of 140,000 (140k) which has considerable amino acid identity with the major immediate early (ie) protein vmw175 (icp4) of herpes simplex virus type i (hsv-1). vmw175 is an essential virus polypeptide with a pivotal role in the activation of early and late viral gene expression and also in the repression of ie gene expression. the vzv 140k protein has been shown to function as a strong transcriptional activator in transfection a ... | 1990 | 2177091 |
| establishment of pcr for the early diagnosis of herpes simplex encephalitis. | the early detection of herpes simplex encephalitis (hse) represents a major problem in viral diagnostics. we have now established a test system based on pcr for the specific amplification of hsv-dna in cerebrospinal fluid (csf) samples followed by oligonucleotide hybridization. the test proved to be very sensitive. five molecules of hsv 1 dna still yielded positive signals after hybridization. the assay was applied to csf samples from 6 patients with confirmed hse. all except one csf sample obta ... | 1990 | 2177775 |
| sensitive enzyme-linked immunosorbent assay for antibody to varicella-zoster virus using purified vzv glycoprotein antigen. | the development of an elisa of increased sensitivity has permitted a more critical evaluation of human humoral immune responses to the live attenuated varicella (oka/merck) vaccine. for use as a solid-phase antigen, the glycoprotein (gp) antigens are prepared by lectin-affinity chromatography from lysates of vzv-infected mrc-5 cells. the lot-to-lot variation in vzv gp content is controlled by standardization of antigen against a panel of human serum providing antigen-coated plates of consistent ... | 1990 | 2177782 |
| varicella vaccine. | 1990 | 2177874 | |
| the value of laboratory testing in uveitis. | accurate diagnosis of uveitis is of great importance since the treatment for the various uveitis entities may differ considerably. in a large number of cases the clinical picture is sufficient to make an adequate diagnosis. there are cases in which the diagnosis cannot be made on clinical grounds alone and support is needed from laboratory tests. only a limited number of tests have been proven to be useful as a diagnostic or prognostic aid. these include hla-b27 typing in patients presenting wit ... | 1990 | 2178095 |
| treatment of acute herpetic neuralgia. a case report and review of the literature. | herpes zoster (shingles) is a viral infection that results from a reactivation of a dormant varicella zoster virus. it has been estimated that more than 300,000 new cases are seen in the united states each year. several factors influence the incidence of infection, with increasing age being the most consistent. postherpetic neuralgia is the no. 1 cause of intractable, debilitating pain in the elderly and is the leading cause of suicide in chronic pain patients over the age of 70. | 1990 | 2186265 |
| varicella with delayed contralateral hemiparesis detected by mri. | we report a 3 1/2-year-old boy who developed a hemiparesis 4 weeks after the onset of a varicella infection. in previously described cases of varicella with delayed contralateral hemiparesis, computed tomography typically revealed infarcts in the basal ganglia and internal capsule. to our knowledge, our patient is the first studied by magnetic resonance imaging. the pathogenesis of this syndrome may be a varicella zoster virus-related vasculopathy similar to that observed in herpes zoster ophtha ... | 1990 | 2187441 |
| varicella-zoster virus disease in patients with human immunodeficiency virus infection. | 1990 | 2200349 | |
| [acute meningitis without cutaneous manifestations caused by varicella zoster virus--intrathecal synthesis of specific antibodies]. | the results of virusologic and cytobiochemical evaluation of csf and serum samples of four patients with acute viral meningitis (avm), most probably induced by varicella zoster virus (vzv), are reported. in no case vzv infection was not cutaneously manifested. aetiologic diagnosis was established according to the presence of specific anti vzv avm, in spite of their presence in the sera. on samples detected by indirect enzyme immunoassay (eia). four different antibody indexes were used to prove t ... | 1990 | 2218729 |
| viral pneumonias. a diagnostic and therapeutic challenge. | viral pneumonias are both a diagnostic and a therapeutic challenge for primary care physicians. the illness should be suspected when an upper respiratory tract infection progresses to include dyspnea and cyanosis. rapid diagnostic tests are now available to detect most of the viruses that cause pneumonias. fortunately, viral pneumonias usually resolve without specific antiviral therapy; however, ribavirin is indicated for respiratory syncytial virus pneumonia in children and ganciclovir sodium ( ... | 1990 | 2235793 |
| intraperitoneal administration of acyclovir in patients receiving continuous ambulatory peritoneal dialysis. | in subjects with normal renal function, acyclovir is rapidly removed from the body via the kidneys. in subjects with end-stage renal disease, the half-life is significantly prolonged. the half-life in subjects receiving hemodialysis and continuous ambulatory peritoneal dialysis (capd) is similarly prolonged (10.0 +/- 2.2 and 13.2 +/- 4.7 hours, respectively). after intravenous dosage, peritoneal clearance was 3.4 +/- 0.2 ml/min. intraperitoneal dosing in subjects receiving capd resulted in a bio ... | 1990 | 2243158 |
| central nervous system vasculitis after chickenpox--cause or coincidence? | a 7.5 year old boy, known to have a seizure disorder, presented with an infarct in the left middle cerebral artery territory, 10 weeks after severe chickenpox. immunofluorescent antibody titre to the varicella zoster virus in the cerebrospinal fluid was 1:32. cerebral angiography showed evidence of focal vasculitis. he presented again seven months later with an acute exacerbation of seizures. magnetic resonance imaging of the brain showed an old posterior extension of the infarct, but a repeated ... | 1990 | 2248537 |
| early relapses of varicella-zoster virus infection in immunocompromised children treated with acyclovir. | authors observed one or more early vzv relapses in 8 out of 98 acyclovir treated immunocompromised children with varicella. none of the 8 children developed vzv antibodies by the end of the 5-day acv treatment. all vzv relapses were successfully treated with acv or vidarabine, but were stopped only after the appearance of vzv antibodies in the patients' sera. the possible role of acv treatment in pathogenesis of early vzv relapses could be excluded by comparing the vzv antibody production of pat ... | 1990 | 2248805 |
| [active immunization against varicella-zoster virus]. | 1990 | 2249295 | |
| transient diabetes following chicken pox. | the paper describes two individuals presenting with acute insulin dependent diabetes mellitus for a brief and transient period. both had had chicken pox infection in the immediate past. after establishing good diabetic control, insulin was withdrawn over a few weeks. follow-up for the next two years did not reveal recurrence of diabetes. a causal relation between varicella zoster virus and the onset of diabetes is suggested. | 1990 | 2266090 |
| [viral diseases of the outer eye--rapid diagnosis by immunohistochemistry]. | rapid diagnosis of viral diseases of the outer eye was attempted by means of immunohistochemical methods. specimens were obtained with a nitrocellulose membrane, used as a blotter of immunoblotting test. an impression of the corneal or conjunctival surface was obtained by anesthetizing the eye and lightly pressing the membrane against the tissues. in some cases, scraping materials were taken on a slide glass. viral antigens in the specimens were detected by peroxidase-antiperoxidase (pap) method ... | 1990 | 2278234 |
| acyclovir-resistant varicella zoster virus infection after chronic oral acyclovir therapy in patients with the acquired immunodeficiency syndrome (aids). | four patients with human immunodeficiency virus (hiv) infection who received chronic oral acyclovir therapy for suppression of recurrent varicella zoster or herpes simplex virus infection developed persistent disseminated hyperkeratotic papules that failed to heal with intravenous or high-dose oral acyclovir therapy. varicella zoster virus, resistant to acyclovir in vitro, was isolated from skin lesions of all four patients. three patients were adults in whom the acquired immunodeficiency syndro ... | 1990 | 2297195 |
| varicella zoster virus transverse myelitis without cutaneous rash. | 1990 | 2337114 | |
| immunoglobulin prophylaxis for infants exposed to varicella in a neonatal unit. | the investigation and prophylaxis with anti-varicella-zoster immunoglobulin (zig) of 15 preterm infants (24-33 weeks gestation and 1-300 days postnatal age) exposed to varicella on a neonatal intensive care unit are described. varicella-zoster virus (vzv) igg was detectable in 13 infants (24-28 weeks gestation) less than 105 days of age and born to seropositive mothers. current guidelines recommend zig for all preterm infants of less than 28 weeks gestation following exposure to vzv. our finding ... | 1990 | 2341729 |
| herpes zoster: etiology, clinical course, and suggested management. | herpes zoster is an acute viral infection that results from reactivation of a latent varicella-zoster virus often acquired as chickenpox during childhood. fifty percent of all people living to the age of 85 will have an attack of zoster. the goal of intervention is to reduce associated pain and discomfort. a prompt diagnosis and appropriate management can best be achieved by understanding the disease and treatment options. while herpes zoster occurs with greater frequency in the geriatric popula ... | 1990 | 2354080 |
| an in vivo model of varicella-zoster virus latent infection of dorsal root ganglia. | we describe here the first in vivo model of varicella-zoster virus (vzv) latent infection in the adult rat peripheral nervous system. infected mewo cells were injected subcutaneously along the spine of healthy adult rats. no clinical sign of infection was observed even 9 months after inoculation. humoral immune response to vzv was detected in all infected animals throughout the study (9 months). the presence of viral material in dissociated and cultured dorsal root ganglia (drg) from inoculated ... | 1990 | 2359148 |
| [granulomatous angiitis of the basilar artery related to herpes zoster of the 7th cranial nerve]. | granulomatous angiitis of the central nervous system is an uncommon condition characterized by vascular wall necrosis, inflammatory exudate and development of giant cells in medium and small size vessels. the pathogenesis of this disease remains unknown, but it has been associated with immune complexes, mechanical factors and infection by the varicella-zoster virus. we report a young patient who presented with herpes zoster involving the vii cranial nerve and contralateral hemiplegia. subsequent ... | 1990 | 2361047 |
| is there an association between fetal viral infection and fetal malformation? i. detection of specific igm antibodies in the serum of malformed fetuses. | determinations of igg and igm antibodies specific for cytomegalovirus (cmv), herpes simplex virus (hsv1), herpes simplex virus (hsv2), varicella-zoster virus (vzv), rubella, echo, coxsackie and morbilli viruses were performed in 20 sera from malformed fetuses. demonstration of a fetal infection by increased fetal serum igm permits linkage to a detected fetal malformation. in parallel, 14 maternal sera and 17 amniotic fluid samples were examined. laser nephelometry (a quantitative method) was use ... | 1990 | 2366130 |
| use of serum substitutes for the growth of four cell lines commonly used for virus isolation. | four serum extenders (opti-mem i, biorich, excyte i and excyte iii) and two serum substitutes (omni serum and serum plus) were evaluated for the reduction or replacement of fbs for the growth and maintenance of four representative cell lines used for virus isolation. mrc-5, human fetal foreskin fibroblast (hf), a549 and hep-2 cells were seeded into culture flasks containing mem with 10% fbs or with the serum extenders or substitutes and subcultured into 16 x 125 mm glass tubes and 1-dram shell v ... | 1990 | 2370288 |