Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| growth of recombinant equine herpesvirus 1 (ehv-1) replaced with passage-induced mutant gene 1 and gene 71 derived from an attenuated ehv-1 in cell cultures and in the lungs of mice. | the relationship of passage-induced mutant genes 1 and 71 of an attenuated equine herpesvirus 1 (ehv-1) with virulence was analysed by constructing nine recombinant ehv-1 viruses by homologous recombination. gene 1 or/and gene 71 of a virulent ehv-1 strain, hh1, was replaced by a mutant gene 1 or/and 71 of an attenuated hh1 strain, bk343, respectively. the beta-galactosidase gene of escherichia coli was inserted within the gene 1 or 71 coding sequence of hh1 to inactivate the genes. virus replic ... | 2003 | 12935744 |
| detection of equine herpesvirus-1 in the fetal membranes of aborted equine fetuses by immunohistochemical and in-situ hybridization techniques. | formalin-fixed, paraffin wax-embedded fetal membranes from 76 cases of equine abortion were examined immunohistochemically for equine herpesvirus (ehv)-1 antigen. of the 76 cases, 11 had been proved ehv-1-positive by diagnostic methods applied to the aborted fetuses (viral isolation in tissue culture, or immunohistochemical examination, or both). of the 11 fetal membranes from the virus-positive animals, five gave positive results on immunohistochemical examination, and three on in-situ hybridiz ... | 2003 | 12921720 |
| detection of ehv-1 and ehv-4 in placental sections of naturally occurring ehv-1- and ehv-4-related abortions in the uk: use of the placenta in diagnosis. | ehv-1 and ehv-4 abortion diagnosis is based upon detailed examination of the aborted fetus. however, in some cases, only the placenta is available for examination. furthermore, the contribution of lesions in the placenta to pathogenesis and diagnosis of ehv-1 and ehv-4 abortion has been neglected. | 2003 | 12875318 |
| in vitro characterisation of high and low virulence isolates of equine herpesvirus-1 and -4. | basic in vitro characteristics of high and low virulence isolates of equine herpesviruses-1 and -4 were investigated with particular reference made to the ab4 and v592 isolates of ehv-1 as both have distinct endotheliotropism and clinical outcomes in pony challenge studies. additionally, some ehv-4 isolates that showed variations in clinical outcome were included in some experiments. the aim of the study was to identify an in vitro characteristic that would differentiate strains of known virulen ... | 2003 | 12801466 |
| multiple determinants contribute to the virulence of hsv ocular and cns infection and identification of serine 34 of the us1 gene as an ocular disease determinant. | the virulence of any given strain of herpes simplex virus (hsv) is probably due to the effects of the constellation of genes in that strain and how they act in concert to promote disease. the goal of this work was to develop a system to identify and study the role of multiple genes in hsv disease. | 2003 | 12766070 |
| comparison of methods for the diagnosis of equine herpesvirus type 1 infection. | the objective of the investigations was to study the occurrence of the equine herpesvirus type 1 (ehv-1) infection in aborted equine fetuses and in newborn foals and to compare the sensitivity of virus isolation, immunohistochemistry and histology in 101 cases and of fetal serology in 68 cases in the diagnosis of the infection. out of the 93 aborted equine fetuses and 8 weak foals, 15 (14.9%) (14 fetuses and 1 foal) proved to be ehv-1 infected by immunohistochemical and 13 (12.9%) by virological ... | 2003 | 12737042 |
| ehv paralytic disease in the south of england. | 2003 | 12708596 | |
| the equine herpesvirus 1 ul11 gene product localizes to the trans-golgi network and is involved in cell-to-cell spread. | experiments were conducted to identify and characterize the equine herpesvirus type 1 (ehv-1) ul11 homologous protein. at early-late times after ehv-1 infection of rk13 cells several proteins at an m(r) of 8000 to 12,000 were detected using a ul11 protein-specific antiserum. particularly, an m(r) of 11,000 protein was found abundantly in purified virions and could be assigned to the tegument fraction. as demonstrated by confocal laser scanning microscopy, ul11 reactivity localized predominantly ... | 2003 | 12706087 |
| herpes simplex virus 1 activates cdc2 to recruit topoisomerase ii alpha for post-dna synthesis expression of late genes. | a subset (gamma(2)) of late herpes simplex virus 1 genes depends on viral dna synthesis for its expression. for optimal expression, a small number of these genes, exemplified by u(s)11, also requires two viral proteins, the alpha protein infected cell protein (icp) 22 and the protein kinase u(l)13. earlier we showed that u(l)13 and icp22 mediate the stabilization of cdc2 and the replacement of its cellular partner, cyclin b, with the viral dna polymerase processivity factor u(l)42. here we repor ... | 2003 | 12665617 |
| divergence of reiterated sequences in a series of genital isolates of herpes simplex virus type 1 from individual patients. | both serotypes of herpes simplex virus (hsv), hsv-1 and hsv-2, are aetiological agents of genital herpes, although genital herpes caused by hsv-1 recurs less frequently. the hsv-1 genome contains a number of short, tandemly repeated sequences, and some reiterated sequences can serve as sensitive markers for the differentiation of hsv-1 strains. in the present study, variation in reiterations (assumed to be due to different copy numbers of tandemly repeated sequences) was examined in hsv-1 isolat ... | 2003 | 12655092 |
| intercellular trafficking and enhanced in vivo antitumour activity of a non-virally delivered p27-vp22 fusion protein. | vp22, a structural protein from herpes simplex virus type i, exhibits the unique property of intercellular trafficking. this protein is exported from primary expressing cells and subsequently imported into neighbouring cells. this property is conserved when vp22 is genetically fused to a protein, making it a promising tool to enhance the delivery of a gene product. we chose to study the intercellular transport and biological effect of a fusion protein between the putative tumour suppressor gene ... | 2003 | 12595890 |
| glycoprotein g isoforms from some alphaherpesviruses function as broad-spectrum chemokine binding proteins. | mimicry of host chemokines and chemokine receptors to modulate chemokine activity is a strategy encoded by beta- and gammaherpesviruses, but very limited information is available on the anti-chemokine strategies encoded by alphaherpesviruses. the secretion of chemokine binding proteins (vckbps) has hitherto been considered a unique strategy encoded by poxviruses and gammaherpesviruses. we describe a family of novel vckbps in equine herpesvirus 1, bovine herpesvirus 1 and 5, and related alphaherp ... | 2003 | 12574120 |
| infected cell protein no. 22 is subject to proteolytic cleavage by caspases activated by a mutant that induces apoptosis. | earlier reports have shown that the d120 mutant of herpes simplex virus 1 lacking both copies of the gene encoding the infected cells protein no. 4 (icp4) induces apoptosis in a variety of cell lines. the programmed cell death induced by this mutant is blocked by overexpression of bcl-2 or by transduction of infected cells with the gene encoding the viral u(s)3 protein kinase. hep-2 cells infected with the d120 mutant express predominantly alpha proteins. studies on these proteins revealed the a ... | 2003 | 12573581 |
| down-regulation of mhc class i expression by equine herpesvirus-1. | there is good evidence that cytotoxic t lymphocytes play an important role in the clearance of equine herpesvirus-1 (ehv1) in horses. we have demonstrated that, in common with other alphaherpesviruses, ehv1 infection can lead to dramatic down-regulation of mhc class i expression at the cell surface, a common strategy for pathogen evasion of the host immune response. this down-regulation is specific for mhc class i and does not reflect a general shut-off of host-cell protein synthesis. the use of ... | 2003 | 12560560 |
| occurrence of infectious upper respiratory tract disease and response to vaccination in horses on six sentinel premises in northern colorado. | horses vaccinated against common agents of infectious upper respiratory disease (iurd) may not have detectable serum antibody and may not be protected from clinical disease. | 2003 | 12553466 |
| interaction of the equine herpesvirus 1 eicp0 protein with the immediate-early (ie) protein, tfiib, and tbp may mediate the antagonism between the ie and eicp0 proteins. | the equine herpesvirus 1 (ehv-1) immediate-early (ie) and eicp0 proteins are potent trans-activators of ehv-1 promoters; however, in transient-transfection assays, the ie protein inhibits the trans-activation function of the eicp0 protein. assays with ie mutant proteins revealed that its dna-binding domain, tfiib-binding domain, and nuclear localization signal may be important for the antagonism between the ie and eicp0 proteins. in vitro interaction assays with the purified ie and eicp0 protein ... | 2003 | 12552007 |
| absence of viral antigens on the surface of equine herpesvirus-1-infected peripheral blood mononuclear cells: a strategy to avoid complement-mediated lysis. | equine herpesvirus-1 (ehv-1) may cause abortion in vaccination- and infection-immune horses. ehv-1-infected peripheral blood mononuclear cells (pbmcs) play an important role in virus immune evasion. the mechanisms by which infected pbmcs can avoid destruction by ehv-1-specific antibody and equine complement were examined. the majority of ehv-1-infected pbmcs (68.6 %) lacked surface expression of viral antigens and these cells were not susceptible to complement-mediated lysis. in infected pbmcs w ... | 2003 | 12533704 |
| requirements for transcriptional repression and activation by engrailed in drosophila embryos. | genetic analysis shows that engrailed (en), a homeodomain-containing transcription factor, has both negative and positive targets. negative regulation is expected from a factor that has a well-defined repressor domain but activation is harder to comprehend. we used vp16en, a form of en that had its repressor domain replaced by the activation domain of vp16, to show that en activates targets using two parallel routes, by repressing a repressor and by being a bona fide activator. we identified the ... | 2003 | 12506003 |
| the development of a competitive pcr-elisa for the detection of equine herpesvirus-1. | equine herpesvirus-1 (ehv-1) infection is of significant animal welfare and economic importance. yet, no standardised molecular techniques are available for diagnosis or confirmation of viral infection. the purpose of this study was to develop a standardised and quantitative assay system for the reliable detection of ehv-1 infection which was capable of eliminating the likelihood of false negative results. a region within the ehv-1 glycoprotein b gene was amplified by polymerase chain reaction ( ... | 2003 | 12505639 |
| mutation of the protein tyrosine kinase consensus site in the herpes simplex virus 1 alpha22 gene alters icp22 posttranslational modification. | we previously reported that at least eight hsv-1 and five hsv-2 proteins were tyrosine phosphorylated in infected human and mouse cells and the first phosphotyrosine-modified gene product identified was the icp22 regulatory protein (blaho, j. a., zong, c. s., and mortimer, k. a., 1997, j. virol. 71, 9828-9832). all electrophoretic forms of icp22 are tyrosine phosphorylated with the exception of the fastest migrating (unmodified) isoform. we now report the following. (i) icp22 that reacted with a ... | 2003 | 12504549 |
| equid herpesvirus (ehv-1) live vaccine strain c147: efficacy against respiratory diseases following ehv types 1 and 4 challenges. | the temperature sensitive and host range mutant clone 147 of equine herpesvirus 1 (ehv-1) was assessed for its ability to protect conventional, susceptible adult horses against respiratory infection by ehv-1 and equine herpesvirus 4 (ehv-4). intranasal (in) vaccination with 5.2 log(10) tcid(50) did not cause adverse clinical reactions although a limited virus shedding and viraemia (leukocytes) was observed in 11 of 15 and 10 of 15 vaccinated horses respectively. all 15 vaccinated horses showed a ... | 2003 | 12488066 |
| contribution of gene products encoded within the unique short segment of equine herpesvirus 1 to virulence in a murine model. | the pathogenesis of three equine herpesvirus 1 (ehv-1) recombinants was assessed in a cba mouse model. sequences encoding the majority of glycoproteins i (gi) and e (ge) were deleted from the pathogenic ehv-1 strain racl11 (l11deltagideltage), and sequences comprising the 3859 bp deletion within the strain kya u(s) segment, which includes genes 73 (gi), 74 (ge), and 75 (putative 10 kda protein 75), were re-inserted into attenuated kya (kgi/ge/75). in addition, genes ge and 75 were inserted into ... | 2002 | 12457983 |
| serum amyloid a (saa) as an aid in the management of infectious disease in the foal: comparison with total leucocyte count, neutrophil count and fibrinogen. | differentiation between infectious and noninfectious disease and rapid initiation of accurate treatment are essential in managing diseases in the neonatal and young foal. identification of useful inflammatory markers for these purposes is, therefore, of great importance. the aim of this study was to compare the responses of the acute phase protein serum amyloid a (saa) with the responses of fibrinogen and total leucocyte and neutrophil counts in infectious diseases encountered in the young foal, ... | 2002 | 12455840 |
| detection and isolation of equine herpesviruses 1 and 4 from horses in normandy: an autopsy study of tissue distribution in relation to vaccination status. | equine herpesviruses type 1 and 4 (ehv-1 and ehv-4) are ubiquitous in the equine population. one of their main properties is their ability to establish life-long latent infections in their hosts even in those with natural or vaccine-induced immunity. however, effect of vaccination status on prevalence and tissue tropism was not established. in this study, ehv-1 and ehv-4 were detected by polymerase chain reaction and by classical virus isolation from neural, epithelial and lymphoid tissues colle ... | 2002 | 12449249 |
| derivation and characterisation of a live equid herpes virus-1 (ehv-1) vaccine to protect against abortion and respiratory disease due to ehv-1. | a german abortion isolate of ehv-1 (strain m8) was grown in equine dermal (ed) cells at a low multiplicity of infection in presence of 5-bromo-2-deoxy uridine. the resulting stock was dialysed, titrated and cloned by terminal dilution in ed cells grown in 96-well microtitration plates. of 192 clones each originating from a single focus, clone 147 (c147) was found to be restricted for growth at and above temperatures of 38.5 degrees c. it was also restricted for growth at 37 degrees c in rabbit k ... | 2003 | 12441229 |
| passage of equine herpesvirus-1 in suckling mouse brain enhances extraneural virus growth and subsequent hematogenous neuroinvasion. | intracerebral inoculation of field-isolates as well as established strains of equine herpesvirus-1 (ehv-1) in suckling mice results in viral replication in neurons and glial cells and induces encephalitis. by intraperitoneal (i.p.) inoculation, no histological lesion was observed in the central nervous system (cns) in suckling mice with the ehv-1 hh1 strain (hh1), whereas a neuroadapted variant (nhh1) produced by serial passage of hh1 in the mouse brain caused severe encephalomyelitis after i.p. ... | 2002 | 12419867 |
| equine herpesvirus-1-induced encephalomyelitis in mice: a comparative study of neuroadapted virus and its parental strain. | little is known about the neuropathogenicity of equine herpesvirus-1 (ehv-1) in mice. no neurological signs were observed in 6-day-old mice inoculated intracerebrally with the hh1 strain (hh1) of ehv-1. however,6-day-old mice inoculated intracerebrally with a variant derived by serial passage of hh1 in mouse brain showed severe neurological symptoms and eventually died. histological analyses were performed on 6-day-old mice inoculated with the neuroadapted hh1 (nhh1) and the parental hh1 strain ... | 2002 | 12354522 |
| the equine herpesvirus 1 ul34 gene product is involved in an early step in virus egress and can be efficiently replaced by a ul34-gfp fusion protein. | the structure and function of the equine herpesvirus type 1 (ehv-1) ul34 homologous protein were characterized. a ul34 protein-specific antiserum reacted with an m(r)28,000 protein that could not be detected in purified extracellular virions. confocal laser scanning microscopy demonstrated that ul34 reactivity mainly concentrated at the nuclear rim, which changed into a punctuate and filamentous pattern at late times after infection. these changes in ul34 distribution were especially prominent w ... | 2002 | 12350350 |
| equid herpesvirus 1 infection of endothelial cells requires activation of putative adhesion molecules: an in vitro model. | antisera to activated equine endothelial cells, which detected surface molecules of 116 kd, 97 kd, 42 kd and 38 kd, were made to investigate the role of endothelial adhesion molecules in equid herpes virus 1 infection. these putative adhesion molecules could be induced by 17-beta oestradiol, chorionic gonadotrophin, or il-2, as well as by lps and pwm. in an in vitro flow system, using equine veins or arteries, equid herpesvirus 1 in leucocytes was only transferred to infect endothelial cells if ... | 2002 | 12165084 |
| serological responses of mares and weanlings following vaccination with an inactivated whole virus equine herpesvirus 1 and equine herpesvirus 4 vaccine. | equine herpesvirus 1 (ehv-1) is a major cause of respiratory disease and abortion in horses worldwide. although some vaccines have been shown experimentally to reduce disease, there are few reports of the responses to vaccination in the field. this study measured antibody responses to vaccination of 159 mares (aged 4-17 years) and 101 foals (aged 3-6 months) on a large stud farm with a killed whole virus ehv-1/4 vaccine used as per the manufacturer's recommendations. using an ehv glycoprotein d ... | 2002 | 12119135 |
| equine herpesvirus 1 and 4 infections: an update. | equine herpesvirus 1 (ehv1) and equine herpesvirus 4 (ehv4) are important ubiquitous equine viral pathogens, causing much damage to the horse industry. ehv1 strains are associated with respiratory disease, abortion, and paresis/paralysis, whereas ehv4 strains are predominantly associated with respiratory disease. in the past decades much research effort has been put into improving knowledge about these viruses. in this paper the current state of knowledge of these viruses and the most important ... | 2002 | 12095082 |
| equid herpesvirus 1 is neurotropic in mice, but latency from which infectious virus can be reactivated does not occur. | equid herpesvirus 1 (ehv-1) is the most common cause of virus-induced abortion in horses. after primary infection the virus becomes latent predominantly in the respiratory tract lymph nodes and the genome can also be detected in the peripheral nervous system. the role of mouse as a feasible model for the establishment of latency and reactivation of ehv-1 was investigated. intracerebral and intranasal infections of 3- and 17-day-old mice were made and virus replication was confirmed by virus isol ... | 2002 | 12061230 |
| identification of two nuclear import signals in the alpha-gene product icp22 of herpes simplex virus 1. | the herpes simplex virus 1 (hsv-1) infected cell protein 22 (icp22) is a multifunctional viral regulator that localizes in the nucleus of infected cells. icp22 is required for optimal virus replication in certain cell types and is subject to extensive posttranslational modification. to map the signals in icp22 which mediate its efficient nuclear localization, we investigated the nuclear import of fusion proteins comprising various fragments of icp22 fused to green fluorescent protein (gfp) or be ... | 2002 | 12033795 |
| the c-terminal regions of the envelope glycoprotein gp2 of equine herpesviruses 1 and 4 are antigenically distinct. | the unusual mucin-like high molecular mass (mr) glycoprotein 2 (gp2) has only been described in the equid alphaherpesviruses, among which there is considerable antigenic cross-reactivity. equine herpesvirus 1 (ehv-1) gp2 is cleaved into a highly glycosylated n-terminal subunit and a 42 kda c-terminal cleavage product. in order to investigate their antigenic recognition by horses naturally infected with ehv-1 and/or equine herpesvirus 4 (ehv-4), the c-terminal cleavage product and high mr gp2 wer ... | 2002 | 11958459 |
| ehv-1 gene63 is not essential for in vivo replication in horses and mice, nor does it affect reactivation in the horse: short communication. | the aim of this study was to investigate the role of immediate early gene (gene63) in the pathogenesis of equine herpesvirus 1 (ehv-1) acute and latent infections in equine and murine models. ehv-1 gene63 mutant virus (g63mut) along with ehv-1 (ab4) was used for intracerebral and intranasal infection of 3 and 17-day-old mice. both viruses were recovered at the same frequency from tissues after infection. two welsh ponies were infected via the intranasal route with each of the viruses. acute infe ... | 2001 | 11942126 |
| equine herpesvirus type 1 (ehv-1) myeloencephalopathy: a case report. | an outbreak of neurological disease occurred in a well-managed riding school. ataxia and paresis were observed in several horses, five of which became recumbent and were euthanized. post-mortem analysis revealed scattered haemorrhages along the spinal cord, that were reflected by multiple haemorrhagic foci on formalin-fixed sections, with the thoracic and lumbar segments being the most affected. pathohistologically, perivascular mononuclear cuffing and axonal swelling, especially in the white ma ... | 2002 | 11911591 |
| cloning of the genomes of equine herpesvirus type 1 (ehv-1) strains kya and racl11 as bacterial artificial chromosomes (bac). | the genome of equine herpesvirus type 1 (ehv-1) strain racl11, a highly virulent isolate obtained from an aborted foal, and that of the modified live vaccine strain kya, were cloned as bacterial artificial chromosomes (bac) in eseherichia coli. mini f plasmid sequences were inserted into the viral genomes by homologous recombination instead of the gene 71 (eus4) open reading frame after co-transfection of viral dna and recombinant plasmid pdelta71-pha2 into rk13 cells. after isolation of recombi ... | 2002 | 11911590 |
| ehv-1 eicp22 protein sequences that mediate its physical interaction with the immediate-early protein are not sufficient to enhance the trans-activation activity of the ie protein. | the early 293 amino acid eicp22 protein (eicp22p) of equine herpesvirus 1 localizes within the nucleus and functions as an accessory regulatory protein (j. virol. 68 (1994) 4329). transient transfection assays indicated that although the eicp22p by itself only minimally trans-activates ehv-1 promoters, the eicp22p functions synergistically with the immediate-early protein (iep) to enhance expression of ehv-1 early genes (j. virol. 71 (1997) 1004). we previously showed that the eicp22 protein enh ... | 2002 | 11900834 |
| increased susceptibility of peripheral blood mononuclear cells to equine herpes virus type 1 infection upon mitogen stimulation: a role of the cell cycle and of cell-to-cell transmission of the virus. | equine herpesvirus-1 (ehv-1) is an important pathogen of horses, causing abortion and nervous system disorders, even in vaccinated animals. during the cell-associated viremia, ehv-1 is carried by peripheral blood mononuclear cells (pbmc), mainly lymphocytes. in vitro, monocytes are the most important fraction of pbmc in which ehv-1 replicates, however, mitogen stimulation prior to ehv-1 infection increases the percentage of infected lymphocytes. the role of the cell cycle in viral replication an ... | 2002 | 11888698 |
| temporary disturbance of actin stress fibers in swine kidney cells during pseudorabies virus infection. | rounding and loosening of cells is a consequence of infection with pseudorabies virus (prv), both in vitro and in vivo. these changes in the normal structure of the cell may be the result of cytoskeletal changes. immunofluorescence staining of actin filaments and microtubule bundles was performed to examine whether prv induces a reorganization of these cytoskeletal components in infected swine kidney (sk) cells. every 2h until 12h post-inoculation (p.i.), cells were washed in cytoskeleton stabil ... | 2002 | 11888692 |
| equine herpesvirus type 1 devoid of gm and gp2 is severely impaired in virus egress but not direct cell-to-cell spread. | experiments were conducted to analyze the effects of a simultaneous deletion of glycoprotein m (gm) and glycoprotein 2 (gp2) of equine herpesvirus type 1 (ehv-1). ehv-1 strain rach was cloned as a bacterial artificial chromosome (prach) by homologous recombination of a mini f plasmid into the unique short region of the genome, thereby deleting gene 71 encoding gp2. upon transfection of the prach dna into rabbit kidney rk13 cells, virus plaques were visible from day 1 after transfection. the muta ... | 2002 | 11886256 |
| herpes simplex virus gene products required for viral inhibition of expression of g1-phase functions. | hsv infection blocks g1 events in the cell cycle and arrests host cell growth in the g1 phase. to further define the mechanism of the effect and determine the viral gene product(s) responsible, we examined various mutant viruses for their effects on cell cycle regulatory proteins (prb, cyclin d1, and cdk4) and on cell cycle progression into s phase. unlike the wild-type virus, the icp27 mutant virus was defective for blocking the phosphorylation of prb proteins, and the normal prb pattern was re ... | 2001 | 11883196 |
| the gene 10 (ul49.5) product of equine herpesvirus 1 is necessary and sufficient for functional processing of glycoprotein m. | the functional cooperation of equine herpesvirus 1 (ehv-1) glycoprotein m (gm) and the gene 10 (ul49.5) product was analyzed. transient-transfection experiments using gm and ul49.5 expression plasmids as well as rk13 cell lines constitutively expressing ul49.5 (rk49.5) or gm (rkgm) demonstrated that the endo-beta-n-acetylglucosaminidase h (endo h)-resistant mature form of gm was detectable only after coexpression of the two proteins. deletion of the ehv-1 ul49.5-homologous gene 10 in strain kya ... | 2002 | 11861861 |
| identification and characterization of marek's disease virus serotype 1 (mdv1) icp22 gene product: mdv1 icp22 transactivates the mdv1 icp27 promoter synergistically with mdv1 icp4. | a previous report [virus genes 6 (1992) 365-378] has shown that the us1 gene of marek's disease virus serotype 1 (mdv1) encodes a homologue of herpes simplex virus type 1 infected cell protein no. 22 (icp22). in the present study, we expressed and identified a product of the mdv1 us1 gene in chicken embryo fibroblasts (cefs) with the aid of a recombinant baculovirus expressing a flag epitope-tagged mdv1 us1 gene, under control of the sralpha promoter (composed of the enhancer region of the simia ... | 2002 | 11856580 |
| polymorphism of open reading frame 71 of equine herpesvirus-4 (ehv-4) and ehv-1. | open reading frame (orf) 71 genes of both equine herpesvirus-1 (ehv-1) and ehv-4 encode a unique glycoprotein, which has been described to vary in molecular mass from 200 to 450 kda. using pcr and nucleotide sequence analysis, it was shown that the orf 71 genes of ehv-1 and ehv-4 are polymorphic due to a variable number of reiterated sequences in two regions, designated regions a and b. region a was threonine-rich and was located near the n terminus. region b comprised a 38 amino acid repeat nea ... | 2002 | 11842247 |
| synthesis and processing of equine herpesvirus 1 glycoprotein d. | previous studies (c. c. flowers and d. j. o'callaghan, 1992, virology 190, 307-315) employed peptide-specific antibodies to identify the product of the glycoprotein d (gd) gene of equine herpesvirus 1 strain kentucky a (kya). gd polypeptides of 55 and 58 kda were detected in ehv-1-infected l-m cells, and the 58-kda protein was observed in the membrane fraction of ehv-1 virions. in this report, the kinetics of synthesis and processing of gd polypeptides are described. one-hour pulse-labeling of e ... | 1995 | 11831735 |
| vaccination of foals and pregnant mares with duvaxyn ehv1, 4 vaccine. | 2002 | 11803056 | |
| a study of the pathogenesis of equid herpesvirus-1 (ehv-1) abortion by dna in-situ hybridization. | the polymerase chain reaction and dna in-situ hybridization were used to study sections of uterine tissue collected from mares near the time of abortion due to equid herpesvirus-1 (ehv-1) infection. these techniques revealed viral nucleic acids in endothelial cells of endometrial arterioles, in accordance with previously published immunohistological data. in addition, however, they revealed nucleic acids in cellular debris within endometrial glands and diffusing across the placenta at sites of m ... | 2001 | 11798247 |
| sequence and genetic arrangement of the u(s) region of the monkey b virus (cercopithecine herpesvirus 1) genome and comparison with the u(s) regions of other primate herpesviruses. | the sequence of the unique short (u(s)) region of monkey b virus (bv) was determined. the 13 genes identified are arranged in the same order and orientation as in herpes simplex virus (hsv). these results demonstrate that the bv u(s) region is entirely colinear with that of hsv type 1 (hsv-1), hsv-2, and simian agent 8 virus. | 2002 | 11773425 |
| the mucosal humoral immune response of the horse to infective challenge and vaccination with equine herpesvirus-1 antigens. | equine herpesvirus-1 (ehv-1) remains a frequent cause of upper respiratory tract infection and abortion in horses worldwide. however, little is known about the local antibody response elicited in the upper airways of horses following exposure to ehv-1. this study analysed the mucosal humoral immune response of weanling foals following experimental infection with virulent ehv-1, or vaccination with either of 2 commercial vaccines. twenty weanlings were assigned to 5 groups and were inoculated wit ... | 2001 | 11770985 |
| neutralizing and complement-fixing monoclonal antibodies as an aid to the diagnosis of equine herpesvirus-1 infection. | one complement-fixing (c-mab) and three complement-dependent neutralizing monoclonal antibodies (n-mabs) were raised against hisar-90-7 equine herpesvirus-1 (ehv-1) strain. the target antigen of the c-mab (2a5) and two of the n-mabs (1h6, 9c4) was identified as a 140 kda polypeptide in western blotting. the target antigen of n-mab (9c6) could not be identified. purified polypeptides of five ehv-1 strains isolated from different regions and at different times gave intense bands at 140 kda when re ... | 2001 | 11767013 |
| duration of immunity induced by an adjuvanted and inactivated equine influenza, tetanus and equine herpesvirus 1 and 4 combination vaccine. | an adjuvanted vaccine containing inactivated equine influenza, herpesvirus antigens, and tetanus toxoid was administered to young seronegative foals of 8 months of age by deep intramuscular injection in the neck (group a). the first two vaccinations were given 4 weeks apart. the third was administered 6 months later. another group of foals (group b) was vaccinated according to the same scheme at the same time with monovalent equine herpes virus (ehv) vaccine (ehv1.4) vaccine. antibody responses ... | 2001 | 11765243 |
| u(s)3 protein kinase of herpes simplex virus 1 blocks caspase 3 activation induced by the products of u(s)1.5 and u(l)13 genes and modulates expression of transduced u(s)1.5 open reading frame in a cell type-specific manner. | the coding domain of the herpes simplex virus type 1 (hsv-1) alpha22 gene encodes two proteins, the 420-amino-acid infected-cell protein 22 (icp22) and u(s)1.5, a protein colinear with the carboxyl-terminal domain of icp22. in hsv-1-infected cells, icp22 and u(s)1.5 are extensively modified by the u(l)13 and u(s)3 viral protein kinases. in this report, we show that in contrast to other viral proteins defined by their properties as alpha proteins, u(s)1.5 becomes detectable and accumulated only a ... | 2002 | 11752164 |
| identification of equine herpesviruses 1 and 4 by polymerase chain reaction. | to develop and validate specific, sensitive and rapid (< 8 hour) diagnostic tests using polymerase chain reaction (pcr) for the diagnosis of abortion and respiratory disease caused by equine herpesvirus 1 (ehv1; equine abortion virus) and ehv4 (equine rhinopneumonitis virus). | 2001 | 11599819 |
| mapping the sequences that mediate interaction of the equine herpesvirus 1 immediate-early protein and human tfiib. | the sole immediate-early (ie) gene of equine herpesvirus 1 encodes a 1,487-amino-acid (aa) regulatory phosphoprotein that independently activates expression of early viral genes. coimmunoprecipitation assays demonstrated that the ie protein physically interacts with the general transcription factor tfiib. using a variety of protein-binding assays that employed a panel of ie truncation and deletion mutants expressed as in vitro-synthesized or glutathione s-transferase fusion proteins, we mapped a ... | 2001 | 11581390 |
| equine herpesvirus 1 glycoprotein d expressed in pichia pastoris is hyperglycosylated and elicits a protective immune response in the mouse model of ehv-1 disease. | equine herpesvirus 1 glycoprotein d (ehv-1 gd) has been shown in mouse models and in the natural host to have potential as a subunit vaccine, using various expression systems that included escherichia coli, baculovirus and plasmid dna. with the aim of producing secreted recombinant protein, we have cloned and expressed ehv-1 gd, lacking its native signal sequence and c-terminal transmembrane region, into the methylotrophic yeast pichia pastoris. the truncated glycoprotein d (gd) gene was placed ... | 2001 | 11551653 |
| a polymerase chain reaction for detection of equine herpesvirus-1 in routine diagnostic submissions of tissues from aborted foetuses. | equine herpesvirus 1 (ehv-1) is the causative agent of abortion, perinatal foal mortality, neurological and acute respiratory diseases in horses. conventional laboratory diagnosis involving viral isolation from aborted foetuses is laborious and lengthy and requires processing of samples within 24 h of collection, which is problematic for samples that come from long distances. the aim of this study was to develop a polymerase chain reaction (pcr) assay useful in argentina to detect dna sequences ... | 2001 | 11471844 |
| pulmonary vasculotropic ehv-1 infection in equids. | 2001 | 11467487 | |
| mitogen stimulation favours replication of equine herpesvirus-1 in equine blood mononuclear cells by inducing cell proliferation and formation of close intercellular contacts. | in the present study, equine herpesvirus-1 (ehv-1)-infected cells were identified in ionomycin/phorbol dibutyrate (iono/pdb)-stimulated peripheral blood mononuclear cells (pbmc) and the mechanism by which stimulation increases the percentage of infected cells was examined. in the population of viral antigen-positive pbmc, 38.4+/-4.5% were cd5(+) t-lymphocytes (18.1+/-3.2% cd4(+) 13.6+/-1.8% cd8(+)), 18.1+/-5.4% were b-lymphocytes, 8.5+/-3.9% were monocytes and 35% remained unidentified. the role ... | 2001 | 11458002 |
| clinical and virological evaluation of the efficacy of an inactivated ehv1 and ehv4 whole virus vaccine (duvaxyn ehv1,4). vaccination/challenge experiments in foals and pregnant mares. | pregnant mares and young foals were vaccinated with duvaxyn ehv1,4, an inactivated and adjuvanted vaccine containing both the ehv-1 and 4 antigens. sn and cf antibody titres were induced two weeks after first vaccination. antibody levels were boosted after second vaccination, however they never reached the levels induced after virus challenge. young foals were challenged with virulent ehv-1 and ehv-4 field viruses. pregnant mares were challenged with the highly abortigenic ehv-1 strain ab4. vacc ... | 2001 | 11457558 |
| molecular characterizations of the equine herpesvirus 1 etif promoter region and translation initiation site. | the equine herpesvirus 1 (ehv-1) homolog of the herpes simplex virus type 1 (hsv-1) tegument phosphoprotein, alphatif (vmw65; vp16), was identified previously as the product of open reading frame 12 (orf12), was shown to trans-activate immediate-early (ie) gene promoters, and was described as a 60-kda virion component designated etif. however, the etif promoter region and transcription initiation site were not identified. the poly(a) signal of the gene 11 (ul49 homolog) lies just upstream of the ... | 2001 | 11448176 |
| deletion of gene 52 encoding glycoprotein m of equine herpesvirus type 1 strain rach results in increased immunogenicity. | the immunogenicity of equine herpesvirus type 1 (ehv-1) strain rach was compared to a rach virus in which gene 52 encoding glycoprotein m (gm) was interrupted by insertion of lacz (hdeltagm-ins) and a rach with 75% of gene 52 was deleted and replaced by lacz (hdeltagm-hs). hdeltagm-ins failed to produce full-length gm, but the carboxy-terminal portion was still expressed. no gm expression was detected in hdeltagm-hs-infected cells. mice were immunised once with 1x10(3) to 1x10(5) plaque-forming ... | 2001 | 11390105 |
| herpes simplex inhibits the capacity of lymphoblastoid b cell lines to stimulate cd4+ t cells. | hsv establish a lifelong persistent infection in their host even among immunocompetent persons. the viruses use a variety of immune evasion strategies, presumably to assist persistent replication in the human host. we have observed that infection of human b lymphoblastoid cells (b-lcl) by hsv resulted in a strong inhibition of their ability to induce cd4(+) t cell clone proliferation and cytokine secretion. this inhibitory effect occurs in a variety of both hsv- and hiv-specific clones from thre ... | 2001 | 11342647 |
| multiple immediate-early gene-deficient herpes simplex virus vectors allowing efficient gene delivery to neurons in culture and widespread gene delivery to the central nervous system in vivo. | herpes simplex virus (hsv) has several potential advantages as a vector for delivering genes to the nervous system. the virus naturally infects and remains latent in neurons and has evolved the ability of highly efficient retrograde transport from the site of infection at the periphery to the site of latency in the spinal ganglia. hsv is a large virus, potentially allowing the insertion of multiple or very large transgenes. furthermore, hsv does not integrate into the host chromosome, removing a ... | 2001 | 11287583 |
| neurological disease associated with ehv-1-infection in a riding school: clinical and virological characteristics. | an outbreak of neurological disease caused by ehv-1 infection is described with emphasis on diagnosis and prognosis for recumbent horses. in april 1995, an outbreak of the neurological form of equine herpesvirus type 1 (ehv-1) occurred in a well-managed riding school with 41 horses: 34 horses showed a temperature spike and 20 some degree of neurological signs, of which 10 were nursed intensively in the indoor arena of the riding school for 3 to 20 days, 8 having to be maintained in slings for 2- ... | 2001 | 11266070 |
| infection of endothelial cells with equine herpesvirus-1 (ehv-1) occurs where there is activation of putative adhesion molecules: a mechanism for transfer of virus. | evidence is presented to show that activation of endothelial and leucoyte adhesion molecules is a key step in transferring virus from infected leucocytes; and determines the restricted tissue tropism. a range of tissues from 2 experimentally infected mares in late pregnancy at 4 and 8 days after infection with ehv-1 were compared with those from normal pregnant and nonpregnant mares. rabbit antisera to equine activated endothelial cell molecules were used to identify which tissues expressed thes ... | 2001 | 11266062 |
| equine herpesvirus myeloencephalopathy in a 14-year-old quarter horse stallion. | a 14-year-old, quarter horse stallion was presented in lateral recumbency, unable to rise. equine herpesvirus myeloencephalopathy was diagnosed, based on presentation, clinical signs, and the ruling out of other possibilities. after initial rapid improvements, ataxia remained, as did chronic cystitis secondary to bladder paralysis. he was euthanized after 2 months. | 2001 | 11265193 |
| egress of alphaherpesviruses: comparative ultrastructural study. | egress of four important alphaherpesviruses, equine herpesvirus 1 (ehv-1), herpes simplex virus type 1 (hsv-1), infectious laryngotracheitis virus (iltv), and pseudorabies virus (prv), was investigated by electron microscopy of infected cell lines of different origins. in all virus-cell systems analyzed, similar observations were made concerning the different stages of virion morphogenesis. after intranuclear assembly, nucleocapsids bud at the inner leaflet of the nuclear membrane, resulting in ... | 2001 | 11264357 |
| neurological signs in a horse due to metastases of an intestinal adenocarcinoma. | a 22-year-old dutch warmblood mare was referred to utrecht university with progressive left hind limb paresis and hyporeflexia. the preliminary clinical diagnosis was the neurological form of equine herpes virus (ehv-1) infection. within 1 day of admission, the mare became recumbent and deteriorated rapidly. postmortem examination revealed an adenocarcinoma of the caecum, with metastases in all regional lymph nodes and extending from the lumbar nodes into the vertebral canal, causing spinal cord ... | 2001 | 11206003 |
| ehv-1 glycoprotein d (ehv-1 gd) is required for virus entry and cell-cell fusion, and an ehv-1 gd deletion mutant induces a protective immune response in mice. | insertional mutagenesis was used to construct an equine herpesvirus 1 (ehv-1) mutant in which the open reading frame for glycoprotein d was replaced by a lacz cassette. this gd deletion mutant (delta gd ehv-1) was unable to infect normally permissive rk cells in culture, but could be propagated in an ehv-1 gd-expressing cell line (rk/gd). phenotypically complemented delta gd ehv-1 was able to infect rk cells, but did not spread to form syncytial plaques as seen with wild type ehv-1 or with delta ... | 2000 | 11205124 |
| characterisation of ie and ul5 gene products of equine herpesvirus 1 using dna inoculation of mice. | the equine herpesvirus 1 (ehv-1) strain hvs25a regulatory genes ie and ul5, encoding homologues of herpes simplex virus 1 (hsv-1) icp4 and icp27 respectively, were cloned into a eukaryotic expression vector and the dna injected intramuscularly into mice. antibodies produced in this way detected the ie or ul5 gene products as diffuse material in nuclei of rk13 cells transfected with the individual genes but as discrete punctate or large aggregates in rk13 cells infected with ehv-1. western blotti ... | 2000 | 11205113 |
| rapid acquisition of entire dna polymerase gene of a novel herpesvirus from green turtle fibropapilloma by a genomic walking technique. | a 4837-bp sequence of a newfound green turtle herpesvirus (gthv), implicated in the etiology of green turtle fibropapilloma, was obtained from tumor tissues of a green turtle with fibropapilloma using a genomic walking method based on restriction enzyme digestion, self-ligation and inverse polymerase chain reaction (ipcr). the 4837-bp sequence was 56.23% g/c rich and contained three nonoverlapping open reading frames (orf). the largest orf (3507-bp) encoded the dna polymerase gene (pol gene), wh ... | 2001 | 11164500 |
| the equine herpesvirus 1 ul45 homolog encodes a glycosylated type ii transmembrane protein and is involved in virus egress. | experiments to analyze the product of the equine herpesvirus type 1 (ehv-1) ul45 homolog were conducted. using an antiserum generated against the carboxylterminal 114 amino acids of the ehv-1 ul45 protein, proteins of m(r) 32,000, 40,000, and 43,000 were detected specifically in ehv-1-infected cells. neither form of the protein was located in purified virions of ehv-1 wild-type strain racl22 or the modified live vaccine strain rach, but ul45 was demonstrated to be expressed as a late (gamma-2) p ... | 2001 | 11145911 |
| the equine herpesvirus 1 immediate-early protein interacts with eap, a nucleolar-ribosomal protein. | the equine herpesvirus 1 (ehv-1) immediate-early (ie) phosphoprotein is essential for the activation of transcription from viral early and late promoters and regulates transcription from its own promoter. the ie protein of 1487 amino acids contains a serine-rich tract (srt) between residues 181 and 220. deletion of the srt decreased transactivation activity of the ie protein. previous results from investigation of the icp4 protein, the ie homolog of herpes simplex virus 1 (hsv-1), revealed that ... | 2001 | 11145900 |
| equine herpesvirus 1 (ehv-1) glycoprotein m: effect of deletions of transmembrane domains. | equine herpesvirus 1 (ehv-1) recombinants that carry either a deletion of glycoprotein m (gm) or express mutant forms of gm were constructed. the recombinants were derived from strain kentucky a (kya), which also lacks genes encoding ge and gi. plaques on rk13 cells induced by the gm-negative kya were reduced in size by 80%, but plaque sizes were restored to wild-type levels on gm-expressing cells. electron microscopic studies revealed a massive defect in virus release after the deletion of gm i ... | 2000 | 11118370 |
| solid matrix-antibody-antigen complexes incorporating equine herpesvirus 1 glycoproteins c and d elicit anti-viral immune responses in balb/c (h-2k(d)) and c3h (h-2k(k)) mice. | glycoproteins c and d (gc and gd) derived from equine herpesvirus 1 (ehv-1)-infected cells were incorporated into individual solid matrix-antibody-antigen (smaa) complexes and administered to balb/c (h-2k(d)) and c3h (h-2k(k)) mice. antibodies against each of the glycoproteins were produced that neutralised virus infectivity and mediated the lysis of ehv-1-infected target cells in the presence of complement. immunoglobulin (ig)g2b was the predominant antibody isotype produced in balb/c mice agai ... | 2000 | 11115713 |
| fatal nonneurological ehv-1 infection in a yearling filly. | a case of fatal nonneurological equine herpesvirus 1 (ehv-1) infection in a yearling filly is described. gross lesions included extensive pulmonary edema, prominent laryngeal lymphoid follicles, and congestion and edema of the dorsal third ventricle choroid plexus. histologically, there was vasculitis, hemorrhage, and edema in the lungs and dorsal third ventricle choroid plexus as well as mild intestinal crypt necrosis with occasional intranuclear inclusion bodies. the perivascular and vascular ... | 2000 | 11105961 |
| prevalence of equine herpesvirus type 1 latency detected by polymerase chain reaction. | in this study, an improved polymerase chain reaction (pcr) was used for detection of dna of latent ehv-1 strains from several sources. three pairs of oligonucleotide primers spanning fragments of 333 bp, 226 bp and 268 bp of the thymidine kinase (tk) gene, and one primer pair spanning 225 bp of the glycoprotein c (gc) gene were used in specific amplifications. primers for ehv-4 pcr were also designed. restriction digests with taqi confirmed the identity of tk pcr fragments from ehv-1. the sensit ... | 2000 | 11043940 |
| demonstration of equine herpesvirus-1 gene expression in the placental trophoblasts of naturally aborted equine fetuses. | equine herpesvirus-1 (ehv-1) infection was demonstrated in the lung tissue of seven aborted fetuses by immunohistochemical labelling and polymerase chain reaction. the placentas of the fetuses were also examined by non-isotopic in-situ hybridization for the ehv-1 glycoprotein b (gb) gene. positive hybridization signals were observed in the cytoplasm of trophoblasts, especially in microcotyledons, of all seven placentas, and in villous epithelium of the allantochorion of six placentas. despite th ... | 2000 | 11032664 |
| differences in determinants required for complex formation and transactivation in related vp16 proteins. | vp16-h is an essential structural protein of herpes simplex virus type 1 (hsv-1) and is also a potent activator of virus immediate-early (ie) gene expression. current models of functional determinants within vp16-h indicate that it consists of two domains, an n-terminal domain involved in recruiting vp16-h to a multicomponent dna binding complex with two host proteins, oct-1 and host cell factor (hcf), and an acidic c-terminal domain exclusively involved in transactivation. vp16-e, from equine h ... | 2000 | 11024140 |
| severe murine lung immunopathology elicited by the pathogenic equine herpesvirus 1 strain racl11 correlates with early production of macrophage inflammatory proteins 1alpha, 1beta, and 2 and tumor necrosis factor alpha. | the cba mouse model was used to investigate the immunopathology induced in the lung by the pathogenic equine herpesvirus 1 (ehv-1) strain racl11 in comparison to infection with the attenuated vaccine candidate strain kya. intranasal infection with kya resulted in almost no inflammatory infiltration in the lung. in contrast, infection with the pathogenic racl11 strain induced a severe alveolar and interstitial inflammation, consisting primarily of lymphocytes, macrophages, and neutrophils. infect ... | 2000 | 11024132 |
| immunization of balb/c mice with dna encoding equine herpesvirus 1 (ehv-1) glycoprotein d affords partial protection in a model of ehv-1-induced abortion. | dna-mediated immunization was assessed in a murine model of equine herpesvirus 1 (ehv-1) abortion. whilst there are differences between the model and natural infection in the horse, literature suggests that ehv-1 infection of pregnant mice can be used to assess the potential ability of vaccine candidates to protect against abortion. female balb/c mice were inoculated twice, 4 weeks apart, with an expression vector encoding ehv-1 glycoprotein d (gd dna). they were mated 15 days after the second i ... | 2000 | 10973696 |
| equine herpesvirus 1 (ehv-1) glycoprotein d dna inoculation in horses with pre-existing ehv-1/ehv-4 antibody. | we have shown previously that equine herpesvirus 1 (ehv-1) glycoprotein d (gd) dna elicited protective immune responses against ehv-1 challenge in murine respiratory and abortion models of ehv-1 disease. in this study, 20 horses, all with pre-existing antibody to ehv-4 and two with pre-existing antibody to ehv-1, were inoculated intramuscularly with three doses each of 50, 200 or 500microg ehv-1 gd dna or with 500microg vector dna. in 8 of 15 horses, inoculation with ehv-1 gd dna led to elevated ... | 2000 | 10946142 |
| application of a type-specific enzyme-linked immunosorbent assay for equine herpesvirus types 1 and 4 (ehv-1 and -4) to horse populations inoculated with inactivated ehv-1 vaccine. | a type-specific enzyme-linked immunosorbent assay (elisa) using equine herpesvirus types 1 (ehv-1) and 4 (ehv-4) glycoprotein g was applied for sero-epizootiology of ehv infections in japan. recently, an inactivated ehv-1 vaccine has been administered to racehorses for prevention of upper respiratory disease. to examine the effect of the vaccination on the result of the elisa, 6 horses were experimentally inoculated three times intramuscularly or intranasally with inactivated ehv-1 vaccine. sera ... | 2000 | 10945284 |
| utilisation of bacteriophage display libraries to identify peptide sequences recognised by equine herpesvirus type 1 specific equine sera. | three filamentous phage random peptide display libraries were used in biopanning experiments with purified igg from the serum of a gnotobiotic foal infected with equine herpesvirus-1 (ehv-1) to enrich for epitopes binding to anti-ehv-1 antibodies. the sequences of the amino acids displayed were aligned with protein sequences of ehv-1, thereby identifying a number of potential antibody binding regions. presumptive epitopes were identified within the proteins encoded by genes 7 (dna helicase/prima ... | 2000 | 10921846 |
| development of a differential multiplex pcr assay for equine herpesvirus 1 and 4 as a diagnostic tool. | in this study, a multiplex polymerase chain reaction (pcr) procedure was developed for differentiation of strains and field isolates of equine herpesvirus type 1 (ehv-1) and type 4 (ehv-4). specific oli-gonucleotide primers were combined to amplify the thymidine kinase (tk) gene region of ehv-1 and ehv-4, which would yield fragments of different lengths for each virus in the same amplification reaction. the specificity of the largest pcr amplicon for ehv-4 was confirmed by restriction digestion ... | 2000 | 10900826 |
| the catalytic subunit of herpes simplex virus type 1 dna polymerase contains a nuclear localization signal in the ul42-binding region. | the herpes simplex virus type 1 dna polymerase consists of a catalytic subunit (pol or ul30) and a processivity factor (ul42). the pol/ul42 interaction, which occurs through the extreme c-terminus of pol, is essential for hsv-1 replication and thus represents a valid target for drug inhibition. we recently showed (a. loregian et al. (1999) proc. natl. acad. sci. usa 96, 5221-5226) that an oligopeptide corresponding to the 27 c-terminal amino acids of pol, when delivered into herpes simplex virus ... | 2000 | 10891416 |
| a rapid and sensitive pcr-based diagnostic assay to detect bovine herpesvirus 1 in routine diagnostic submissions. | we describe a rapid, sensitive and specific polymerase chain reaction (pcr) assay for the detection of bhv1 dna in a range of routine diagnostic submissions without the need for prior virus isolation. the assay, which is based on the selected amplification of a portion of the viral tk gene, detected both bhv1.1 and bhv1.2 subtypes in a panel of 15 characterised field isolates, and its sensitivity was estimated to be <0.125 tcid(50). bhv2, alcephaline herpesvirus, bhv4, equine herpesvirus 1 (ehv1 ... | 2000 | 10889405 |
| equid herpesvirus 1: platelets and alveolar macrophages are potential sources of activated tgf-b1 in the horse. | cell mediated responses to equid herpesvirus 1 (ehv-1) are of short duration in vivo and require considerable expansion to be detected in vitro. raised serum levels of active transforming growth factor b (tgf-b1) have been shown to depress proliferative t cell responses in experimental infections with ehv-1 in ponies. the present work indicates that latent transforming growth factor b (tgf-b1) is present in circulating platelets, lymph node, bronchial epithelium and alveolar macrophages. activat ... | 2000 | 10889300 |
| pseudorabies virus glycoprotein m inhibits membrane fusion. | a transient transfection-fusion assay was established to investigate membrane fusion mediated by pseudorabies virus (prv) glycoproteins. plasmids expressing prv glycoproteins under control of the immediate-early 1 promoter-enhancer of human cytomegalovirus were transfected into rabbit kidney cells, and the extent of cell fusion was quantitated 27 to 42 h after transfection. cotransfection of plasmids encoding prv glycoproteins b (gb), gd, gh, and gl resulted in formation of polykaryocytes, as ha ... | 2000 | 10888614 |
| incidence of equine herpesvirus 1 infection in thoroughbred weanlings on two stud farms. | 2000 | 10840577 | |
| virulence of the v592 isolate of equid herpesvirus-1 in ponies. | the v592 strain of equid herpesvirus-1 (ehv-1), which was originally isolated from a fetus during an abortion epizootic, has proved to be of low virulence in infection studies. five welsh mountain pony mares and one foal were challenged intranasally or by aerosol with this isolate, and monitored clinically and virologically. all six animals shed virus in nasopharyngeal mucus, and viraemia was recorded from day 7 post-infection (pi). pathological investigations revealed mild rhinitis and bronchio ... | 2000 | 10805982 |
| differentiation and genomic and antigenic variation among fetal, respiratory, and neurological isolates from ehv1 and ehv4 infections in the netherlands. | ten monoclonal antibodies (mabs) were produced against equine herpes virus type 1 (ehv1). two appeared type-specific, while the other eight were directed against epitopes common to both ehv1 and ehv4. two mabs directed against the glycoprotein gp2 recognized linear epitopes, as demonstrated by western blotting. with pools of type-specific mabs, 282 field isolates were typed in an immunoperoxidase monolayer assay (ipma). from a total of 254 fetal or neonatal isolates, 244 (96%) were typed as ehv1 ... | 2000 | 10789516 |
| an equine herpesvirus 1 (ehv1) abortion storm at a riding school. | an outbreak of ehv1 abortions occurred at a riding school in the netherlands in 1991. seven of twelve pregnant mares aborted, and another foal died at 8 days of age. six abortions occurred within 12 days in march after an initial abortion on 8 february. four mares delivered live foals. virological examination of four aborted foals revealed an ehv1 infection. serological results for paired sera from 17 horses suggested, that the initial abortion on 8 february was the index case, and probably caus ... | 2000 | 10789515 |
| efficacy of an inactivated vaccine for equine herpesvirus type 1 in a novel hamster model. | a vaccine designated f.ehv1(s(-))bhk, which was prepared by formaldehyde treatment of equine herpesvirus type 1 (ehv1)-infected baby hamster kidney cells, stimulated neutralising antibodies in hamsters and rabbits and protected new-born hamsters against a lethal challenge with ehv1 by vaccination of their pregnant mothers. the preparative method was then modified to eliminate virus particles and intraparticulate dna, producing a vaccine designated bg.f.ehv1(s(-))bhk. this modification stimulated ... | 2000 | 10773735 |
| ataxia and paresis with equine herpesvirus type 1 infection in a herd of riding school horses. | an outbreak of neurologic disease associated with serologic evidence of equine herpesvirus type 1 (ehv-1) infection occurred in a herd of 46 riding school horses. ataxia and paresis were observed in 14 geldings and 5 barren mares. eight affected horses had distal limb edema, 1 horse had a head tilt, and 3 others had urinary incontinence. other clinical signs included fever, depression, and inappetance in 30 horses. seven horses with neurologic signs were treated with acyclovir. serum neutralizin ... | 2000 | 10772493 |
| equine immunity to viruses. | the identification of some of the adaptive immune responses to infection with equine viruses has been the first step toward rational immunoprophylactic design. sufficient knowledge of infection-induced immunity and informed estimates of the requirements for long-term immunity for eiv have now been obtained. thus, the future for inactivated eiv vaccines is promising now that new adjuvants have been applied to induce cellular immunity and safe methods have been designed to stimulate virus-neutrali ... | 2000 | 10752138 |
| [relevance of infection with equine herpesvirus 1 (ehv-1) in a german thoroughbred stud: vaccination, abortion and diagnosis]. | the aim of the present study was to clarify whether an ehv-1 induced abortion can be prognosticated by an increase of antibody titres, virus shedding and/or viraemia and whether the current abortion diagnostic is suitable. in this context the immune response post immunization and a possible reactivation were of great interest. for this purpose blood samples of 32 mares between the ages of 5-21 years were regularly investigated during a period of two years before and after vaccination and pregnan ... | 2000 | 10726362 |
| shivering in a thoroughbred mare. | an 11-year-old mare presented with neuromuscular deficits and what resembled shivering in the left hind limb. on necropsy, there was no evidence of denervation atrophy of the left hind gastrocnemius muscle. the spinal cord had a small, right-sided lesion at c3-c4 and c4-c5. tests for equine herpesvirus-1 and sarcocystis spp. were negative. | 2000 | 10723600 |
| quantitation of virus-specific classes of antibodies following immunization of mice with attenuated equine herpesvirus 1 and viral glycoprotein d. | the antibody responses of cba/j mice infected intranasally (i.n.) with either the attenuated kya strain or the pathogenic racl11 strain of equine herpesvirus 1 (ehv-1) or immunized with recombinant glycoprotein d (rgd) were investigated using the elispot assay to measure ehv-1-specific antibody-secreting cells (asc) in the regional lymphoid tissue of the respiratory tract. igg, iga, and igm asc specific for ehv-1 were detected in the mediastinal lymph nodes (mln) and lungs 2 weeks after i.n. inf ... | 2000 | 10704356 |