Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| identification of the gb homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies. | equine herpesvirus types 1 and 4 (ehv-1 and ehv-4) labelled with [14c]glucosamine were purified from infected cell culture medium and profiles of their structural proteins were obtained that enabled identification of the major glycoproteins. nine glycosylated polypeptides were identified for each virus. preparations of the purified viruses each contained a glycoprotein which was linked by disulphide bonds, as determined by diagonal gel electrophoresis under reducing/non-reducing conditions. high ... | 1989 | 2543773 |
| the characterization of neutralizing and non-neutralizing monoclonal antibodies against equid herpesvirus type 1. | seven monoclonal antibodies (mabs) were produced which recognized equid herpesvirus type 1 (ehv-1). three mabs neutralized the subtype 1 virus (strain army 183) in the presence of complement, but did not neutralize the subtype 2 virus (strain md). all three mabs immunoprecipitated an mr 83k glycoprotein from a detergent-solubilized virion envelope preparation of the subtype 1 virus. the target antigens of the four non-neutralizing mabs (6f11, 2a4, 1f10 and 8d9) were identified by immunoblotting ... | 1989 | 2543748 |
| identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gb. | a gene in equine herpesvirus 1 (ehv-1; equine abortion virus) equivalent to the gb glycoprotein gene of herpes simplex virus (hsv) has been identified by dna hybridization and nucleotide sequencing. a 4.3 kbp ehv-1 psti-clai sequence (0.40 to 0.43 map units) contained an open reading frame flanked by appropriate control elements and was capable of encoding a polypeptide of 980 amino acids. this had 50 to 60% identity over a 617 amino acid conserved region with the gb gene products of hsv and thr ... | 1989 | 2543744 |
| equine herpesvirus outbreaks. | 1989 | 2543120 | |
| ehv-1: a recurrent problem. | 1989 | 2543119 | |
| equine rhinopneumonitis. | 1989 | 2543118 | |
| hemin and cyclic amp stimulate message-dependent translation in lysates from friend erythroleukemia cells. | a message-dependent, cell-free translation system was prepared from both uninduced and n,n'-hexamethylene-bis-acetamide-induced friend erythroleukemia cells following modification of standard protocols. active extracts were prepared by lysing friend erythroleukemia cells in hypotonic buffer containing 50 microm hemin and 10 mm cyclic adenosine monophosphate (camp), and assaying translation in vitro in the absence of exogenous nucleoside triphosphates. both hemin and camp were required for full a ... | 1989 | 2541008 |
| [training of the immune system of foals against erp virus infections by frequent vaccination with presently available commercial vaccines]. | during 3 foaling seasons around 150 lipizzaner foals were vaccinated against erp with commercial vaccines and groups thereof were serotested in cf and sn for their humoral immune response. in addition, 6 horses of cheaper common breeds were vaccinated on the university premises, were continuously serologically screened and subjected to virulent nasal test infection. the live-virus vaccine prevaccinol interfered so profoundly and up to the 20th week of life with maternal antibodies that its furth ... | 1989 | 2540944 |
| serum amyloid a protein (saa) in horses: objective measurement of the acute phase response. | a sensitive and precise immunoassay for equine serum amyloid a protein (saa) was established and used to determine, for the first time, the circulating concentration of this protein in health and disease. as in other species, equine saa was present only at trace levels in healthy animals but behaved as an extremely sensitive and rapidly responding acute phase reactant following most forms of tissue injury, infection and inflammation, objectively reflecting the extent and activity of disease. mea ... | 1989 | 2539996 |
| a neutrophil-derived antiviral protein: induction requirements and biological properties. | polymorphonuclear neutrophilic granulocytes (pmn) have been implicated as playing a role in antiviral defense. in addition to having phagocytic and cytotoxic activities, pmn may produce an antiviral substance with interferon (ifn)-like activity. the product, for which the name polyferon (pf) has been coined, is produced upon direct encounter of pmn with bovine herpesvirus 1 (bhv-1)-infected bovine cells or membranes thereof. exposure to purified virus only does not induce pf. the intimate intera ... | 1989 | 2539494 |
| latency of equine herpesvirus 4. | 1989 | 2538953 | |
| monoclonal antibodies with neutralizing activity to equine herpesvirus 1. | seven monoclonal antibodies with neutralizing activity to equine herpesvirus-1 were produced. these recognized either an 81 kd or an 88 kd viral glycoprotein. some of the antibodies were shown to protect hamsters in a passive immunization experiment. | 1989 | 2538111 |
| functional mapping and dna sequence of an equine herpesvirus 1 origin of replication. | the genome of equine herpesvirus 1 (ehv-1) defective interfering (di) particle dna originates from discrete regions within the standard (std) ehv-1 genome: the left terminus (0.0 to 0.04 map units) and the inverted repeats (0.78 to 0.79 and 0.83 to 0.87 map units of the internal inverted repeat; 0.91 to 0.95 and 0.99 to 1.00 map units of the terminal inverted repeat). since di dna must contain cis-acting dna sequences, such as replication origins, which cannot be supplied in trans by the std ehv ... | 1989 | 2536833 |
| equine interferons following exposure to equid herpesvirus-1 or -4. | when 23 ponies were infected with equid herpesvirus-1 or -4 (ehv-1 or ehv-4), nasal shedding of interferon (ifn) correlated closely with the duration of viral excretion. equine interferon (eqifn) was detected in the serum only from animals infected with the ehv-1 virus, and here high levels correlated with clinical symptoms of locomotor disorder and indicated a poor prognosis. low levels of ifn were detected in explanted mononuclear cells from ponies infected with either virus. | 1989 | 2474039 |
| a hamster model of equine herpesvirus type 1 (ehv-1) infection; passive protection by monoclonal antibodies to ehv-1 glycoproteins 13, 14 and 17/18. | following intraperitoneal or intranasal inoculation of the syrian hamster with equine herpesvirus type 1 (ehv-1), strain kentucky d, virus replicated in the liver and lungs reaching a peak at 4 days post-infection (p.i.). by day 6 p.i. virus titres in these organs had reduced and the spleen contained virus-specific cytotoxic cells. this cytotoxicity was mediated by t cells since treatment of effector cells with a monoclonal antibody to hamster t lymphocytes inhibited the effect. an antiviral hum ... | 1989 | 2471805 |
| characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gp13) with homology to herpes simplex virus glycoprotein c. | the molecular structure of the equine herpesvirus type 1 (ehv-1) gene encoding glycoprotein 13 (gp13) was analyzed. the gene is contained within a 1.8-kilobase acci-ecori restriction fragment mapping at map coordinates 0.136 to 0.148 in the ul region of the ehv-1 genome and is transcribed from right to left. determination of the nucleotide sequence of the dna fragment revealed a complete transcriptional unit composed of typical regulatory promoter elements upstream to a long open reading frame ( ... | 1988 | 2455821 |
| rapid diagnosis and characterization of equid herpesvirus 1 using monoclonal antibodies. | twelve monoclonal antibodies (mabs) were produced against the attenuated rach subtype 1 strain of equid herpesvirus 1. nine were subtype specific by immunofluorescence or immunoperoxidase, while the other three were against epitopes common to both subtypes. use of these mabs allowed isolates to be rapidly and easily subtyped. immunoblotting indicated that the subtype common mabs were against viral proteins of mw 140 k and 90 k (vp 9 and 14), while the subtype-specific mabs were against the glyco ... | 1987 | 2438808 |
| innate immunity during equid herpesvirus 1 (ehv-1) infection. | intrinsic phagocytosis and killing of c. albicans by equine monocytes and polymorphonuclear leucocytes (pmn) was examined during equid herpesvirus 1 (ehv-1) (subtypes 1 or 2) and adenovirus infections. monocyte function increased during ehv-1 subtype 2 and adenovirus infection. conversely, there was an impairment of monocyte ingestion during ehv-1 subtype 1 infection which was ascribed to virus replication in peripheral blood mononuclear cells. pmn phagocytosis was not decreased in any of the in ... | 1986 | 2431815 |
| infarction of the pons and medulla oblongata caused by arteriolar thrombosis in a horse. | infarction of the pons and rostral medulla secondary to arteriolar thrombosis was documented histologically in a 17-year-old mixed-arabian female horse. clinically, the animal experienced a sudden onset of a head tilt and subsequent non-controllable seizures. there was no historical, clinical or histological evidence to suggest the presence of infection of equine herpesvirus-1 or the feeding of corn contaminated by fusarium moniliforme. | 1990 | 2364707 |
| the immunodominant glycoprotein complex of equid herpesvirus 1 (ehv-1) and the counterpart of ehv-4. | 1990 | 2177803 | |
| antiviral activity of 9-(2-hydroxyethoxymethyl) guanine against equine herpesvirus type 1 hh-1 strain and pseudorabies virus ys-81 strain. | 1990 | 2177802 | |
| equine herpesvirus type 1 unique short fragment encodes glycoproteins with homology to herpes simplex virus type 1 gd, gi and ge. | the nucleotide sequence of a 6.4 kbp portion of the 10.6 kbp bamhi fragment d contained in the unique short region of the equine herpesvirus type 1 (ehv-1) genome has been determined. analysis of this sequence revealed five open reading frames (orfs), four complete and one incomplete, which were encoded by the same sense strand. comparison of the ehv-1 dna sequence with that encoding glycoproteins of other alphaherpesviruses has revealed no significant homologies. comparison at the amino acid le ... | 1990 | 2177089 |
| protection against lethal equine herpes virus type 1 (subtype 1) infection in hamsters by immune stimulating complexes (iscoms) containing the major viral glycoproteins. | an experimental iscom vaccine has been prepared from gradient purified equine herpes virus type 1 (ehv-1). radiolabelling studies demonstrated that this vaccine contained all the major viral glycoproteins in relative amounts similar to those found in non-detergent disrupted viral preparations. this ehv-1 iscom vaccine generated fully protective responses in hamsters challenged with an otherwise lethal dose of the hamster-adapted ehv-1 strain rach. | 1990 | 2174599 |
| organization and function of the oris sequence in the genome of ehv-1 di particles. | equine herpesvirus type 1 (ehv-1) cultures enriched for defective interfering particles (dip) mediate oncogenic transformation and persistent infection in permissive hamster embryo fibroblasts. we have recently demonstrated that an origin of replication (ori) is located within the central portion (map units 0.828 and 0.948) of the inverted repeat sequence (irs) of the short region of the standard ehv-1 genome. in the generation of the genome of ehv-1 di particles, sequences from this internal po ... | 1990 | 2173265 |
| characterization of the high mr glycoprotein (gp300) of equine herpesvirus type 1 as a novel glycoprotein with extensive o-linked carbohydrate. | the high mr glycoprotein (gp300) of equine herpesvirus type 1 was found to have an mr, estimated by sds-page, of over 400,000 and was confirmed as being a surface glycoprotein by 125i-labelling. in contrast to [3h]glucosamine, gp300 showed very low levels of [3h]glucosamine, gp300 showed very low levels of [3h]mannose incorporation. the mr of gp300 showed no detectable change upon treatment of purified virus with n-glycanase, and showed only a small change in virus-infected cells treated with tu ... | 1990 | 2172454 |
| the nucleotide sequence of the equine herpesvirus 4 gc gene homologue. | the genomic position of an equine herpesvirus 4 (ehv-4) gene homologue of the herpes simplex virus 1 (hsv-1) gc gene was determined by southern analysis and dna sequencing. the gene lies within a 2-kbp bg/ii-ecori fragment mapping between 0.15 and 0.17 within the long unique component of the ehv-4 genome and is transcribed from right to left. putative promoter elements were identified upstream of the 1455-bp open reading frame which encodes a 485-amino-acid protein of unglycosylated molecular we ... | 1990 | 2171212 |
| [enzootic viral abortion on a stud farm in east switzerland]. | an outbreak of abortion due to the equine herpesvirus-1 (ehv-1) in the eastern part of switzerland is reviewed. seven of eleven pregnant mares aborted within twenty-three days in january 1989. four weeks later another foal died a few minutes after parturition. three mares delivered live foals in february, march and april without any complications. the examination of the eight dead foals revealed an ehv-1 infection. the clinical signs and the pathology are discussed. severe complications during t ... | 1990 | 2171142 |
| comparative studies of the proteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3: antibody response of the natural hosts. | proteins of purified virions of equine herpesvirus 4 (ehv-4; equine rhinopneumonitis), ehv-1 (equine abortion virus) and asinine herpesvirus 3 (ahv-3) were compared by metabolic labelling with [35s]methionine or [14c]glucosamine during growth of low passage virus in natural host cells (horse or donkey) and high passage virus in an appropriate cell line and analysis by sds-page. approximately 25 different proteins (mr 300k to 21.5k) were clearly resolved for each virus. the three viruses had simi ... | 1990 | 2170572 |
| the nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein h gene. | the equine herpesvirus 4 (ehv-4) gene glycoprotein h (gh) gene homologue was localized by virtue of the conserved genomic position of this gene throughout members of the herpesvirus family. the gene maps immediately downstream of the thymidine kinase gene at approximately 0.49 to 0.51 map units within genomic fragment bamh1 c. the ehv-4 gh primary translation product is predicted to be a polypeptide of mr 94,100, 855 amino acids long, which possesses features characteristic of a membrane glycopr ... | 1990 | 2167933 |
| equine herpesvirus type 1: detection of viral dna sequences in aborted fetuses with the polymerase chain reaction. | primers and probes were selected from the gene encoding glycoprotein 13 (gp 13) of equine herpesvirus 1 (ehv-1). the polymerase chain reaction (pcr) was run on infected and noninfected cultured cells and on 63 specimens from 29 aborted equine fetuses. the results were evaluated by electrophoresis and dot-blot hybridization using an oligonucleotide probe labeled with biotin. in the infected samples electrophoresis showed a pcr product of about 280 base pairs. the dot-blot hybridization confirmed ... | 1990 | 2163562 |
| viraemia and abortions are not prevented by two commercial equine herpesvirus-1 vaccines after experimental challenge of horses. | eighteen horses, vaccinated on a number of occasions over a period of 12 to 20 months with either a live equine herpesvirus-1 (ehv-1) or an inactivated ehv-1 vaccine, were challenged by the intranasal instillation of the subtype 1 virus isolated from the 1983 outbreak of abortion and paralytic disease at the lipizzan stud, piber, austria. the prechallenge serum titres of all vaccinated horses were remarkably low, although most horses had received their last vaccine dose only 3 weeks before test- ... | 1990 | 2163560 |
| effective chemotherapy of equine herpesvirus 1 by phosphonylmethoxyalkyl derivatives of adenine demonstrated in a novel murine model for the disease. | equine herpesvirus 1 was established in adult mice by means of intranasal inoculation. a disease developed that showed several features closely resembling the infection in the natural host. these included the restriction of virus replication to the respiratory tract and blood, the replication of virus in ciliated mucosa, and development of viremia for several days during the acute phase of the infection. infected mice were treated with the antiviral agent (s)-9-(3-hydroxy-2-phosphonylmethoxyprop ... | 1990 | 2163242 |
| safety and efficacy of a thymidine kinase negative equine herpesvirus-1 vaccine in young horses. | a drug induced equine herpesvirus-1 (ehv-1) mutant lacking thymidine kinase inducing activity was developed and evaluated as a vaccine. the safety and effectiveness of the vaccine to protect against experimentally induced ehv-1 respiratory disease were evaluated in weanling horses free of ehv-1 neutralizing antibody. the vaccine was safe when administered either intramuscularly or intravenously, and ehv-1 was not shed intranasally during the 12 days following administration. intranasal challenge ... | 1990 | 2162730 |
| inhibition of equine herpesvirus type 1 subtype 1-induced ribonucleotide reductase by the nonapeptide yagavvndl. | the synthetic nonapeptide yagavvndl [identical to the nine carboxy-terminal amino acids of the small subunit of herpes simplex virus (hsv)-encoded ribonucleotide reductase (rr)] was found to inhibit the rr activity induced by equine herpesvirus type 1 subtype 1 (ehv-1). parallel experiments with hsv type 1 (hsv-1)-encoded rr established that the concentration of peptide required to inhibit 50% of the rr activity was 28 microm for both enzymes. the optimum ph for the ehv-1 enzyme was found to be ... | 1990 | 2161904 |
| the pathogenesis of equine herpesvirus type 1 in the mouse: a new model for studying host responses to the infection. | an infection was established in adult balb/c mice by means of intranasal inoculation of the ab4 strain of equine herpesvirus type 1 (ehv-1). the acute infection was confined to the respiratory tract and blood. virus was shown to replicate in the nasal mucosa, trachea and lung for several days producing clinical signs of disease. viraemia was also detected and a small proportion of peripheral blood cells contained virus at the peak of the infection. histological and electron microscopic evidence ... | 1990 | 2161048 |
| transcript analysis of the equine herpesvirus 1 glycoprotein b gene homologue and its expression by a recombinant vaccinia virus. | transcript mapping of the equine herpesvirus 1 (ehv-1) glycoprotein b (gb) gene homologue by northern blot, s1 nuclease and primer extension analyses indicated that two overlapping transcripts of 3.4 and 4.6 kb originated from the same strand and were transcribed from left to right between coordinates 0.40 and 0.43 of the ehv-1 genome. the 3.4 kb transcript encoded ehv-1 gb and the 5' rna terminus was located approximately 30 bases downstream from a probable tata element. the coding region of th ... | 1990 | 2161047 |
| haematological measurements as an aid to early diagnosis and prognosis of respiratory viral infections in thoroughbred horses. | in late november 1988 large numbers of thoroughbred horses in training in hong kong developed a transient pyrexia with, in some cases, the clinical signs of viral respiratory disease. serial blood samples for haematological examination were taken from 10 of the horses which were stabled in six different blocks. they had developed a high temperature within three days of each other and subsequently seroconverted to equine herpes virus 1 (ehv1). the absolute monocyte count was more than 0.5 x 10(9) ... | 1990 | 2159670 |
| one way protection between equid herpesvirus 1 and 4 in vivo. | two groups each of six sibling ponies were exposed to sequential infections with equid herpesvirus 1 or 4 (ehv-1 or ehv-4) at four or five month intervals. two exposures to ehv-4 did not significantly reduce virus shedding or pyrexia when the ponies were subsequently exposed to ehv-1. however, two sequential infections with ehv-1 completely protected against challenge with ehv-4. virus neutralising antibody in each group did not increase until 21 days after primary exposure and was subtype speci ... | 1990 | 2159176 |
| identification of the site of recombination in the generation of the genome of di particles of equine herpesvirus type 1. | defective interfering particles (dips) are generated by serial, undiluted propagation of equine herpesvirus type 1 (ehv-1). dip-rich preparations of ehv-1 mediate oncogenic transformation and persistent infection in permissive hamster embryo fibroblasts. the defective genomes consist of reiterations of sequences from the left terminus (0.00 to 0.04 map units) of the long (l) region covalently linked to sequences from the inverted repeats (0.78 to 0.79, 0.83 to 0.87, 0.91 to 0.95, and 0.99 to 1.0 ... | 1990 | 2158182 |
| coexpression by vaccinia virus recombinants of equine herpesvirus 1 glycoproteins gp13 and gp14 results in potentiated immunity. | the equine herpesvirus 1 glycoprotein 14 (ehv-1 gp14) gene was cloned, sequenced, and expressed by vaccinia virus recombinants. recombinant virus vp613 elicited the production of ehv-1-neutralizing antibodies in guinea pigs and was effective in protecting hamsters from subsequent lethal ehv-1 challenge. coexpression of ehv-1 gp14 in vaccinia virus recombinant vp634 along with ehv-1 gp13 (p. guo, s. goebel, s. davis, m. e. perkus, b. languet, p. desmettre, g. allen, and e. paoletti, j. virol. 63: ... | 1990 | 2157895 |
| sequence and organization of the genomic termini of equine herpesvirus type 1. | the nucleotide sequence and organization of the genomic termini and of the junction of the long (l) and short (s) regions of the equine herpesvirus type 1 genome were determined. sequencing of the xbai-q fragment (1441 nucleotides) revealed that the left terminus contains sets of inverted repeat and direct repeat sequences. the terminal sequence is described as dr1-uc-dr4 (18, 60, and 16 nucleotides, respectively) because of its homology to these elements of the 'a' sequence of herpes simplex vi ... | 1990 | 2157316 |
| temporal regulation of equine herpesvirus type 3 transcription. | the transcription of equine herpesvirus type 3 (ehv-3; equine coital exanthema virus) has been examined and found to be temporally regulated into three classes: immediate early (ie), early (e), and late (l). hybridization of in vivo 32po4-labeled transcripts revealed that ie transcript(s) are derived exclusively from the inverted repeat segments (irs) of the viral genome, while e and l transcripts are not restricted to any specific region of the genome. northern blot analysis of ehv-3 ie rna rev ... | 1990 | 2157315 |
| genomic termini of equine herpesvirus 1. | after cell infection with the equine herpesvirus 1 (ehv-1), the termini of the linear double-stranded dna genome fuse to form circular forms. to investigate the mechanisms in the generation and cleavage of such replicative-form dnas, the genomic termini, the fusion of termini from replicative-form molecules, and the junction between the short and long genome segments have been analyzed by restriction mapping, blot hybridizations, cloning, and sequencing. the data suggest that the genome ends are ... | 1990 | 2153249 |
| three-dimensional structures of maturable and abortive capsids of equine herpesvirus 1 from cryoelectron microscopy. | cryoelectron microscopy and three-dimensional computer reconstruction techniques have been used to compare the structures of two types of dna-free capsids of equine herpesvirus 1 at a resolution of 4.5 nm. "light" capsids are abortive, whereas "intermediate" capsids are related to maturable intracellular precursors. their t = 16 icosahedral outer shells, approximately 125 nm in diameter, are indistinguishable and may be described in terms of three layers of density, totalling 15 nm in thickness. ... | 1990 | 2153224 |
| equine herpes virus 1 and pseudorabies virus resistance to 2'-fluoropyrimidine analogs and to bromovinyldeoxyuridine: implications for dtmp kinase activity. | the 2'-fluoropyrimidine nucleoside analogs 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac). 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau), and 1(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau) showed higher in vitro activity against herpes simplex virus type 1 (hsv-1), than equine herpesvirus 1 (ehv-1) or pseudorabies virus (prv). comparison of the 50% plaque inhibitory doses for hsv-1 and its mutant mmdur-20 in cell cultures with inhibition c ... | 1990 | 1983184 |
| effects of human alpha interferon on experimentally induced equine herpesvirus-1 infection in horses. | the immunotherapeutic effect of low-dose human alpha interferon on viral shedding and clinical disease was evaluated in horses inoculated with equine herpesvirus-1 (ehv-1). eighteen clinically healthy weanling horses, 5 to 7 months old, were allotted to 3 equal groups. two groups were treated orally with human alpha-2a interferon (0.22 or 2.2 u/kg of body weight), on days 2 and 1 before inoculation with ehv-1, the day of inoculation, and again on postinoculation day 1. the horses of the remainin ... | 1990 | 1964771 |
| structure and physical map of the genome of parma wallaby herpesvirus. | the dna of parma wallaby herpesvirus (macropodid herpesvirus 1; mhv-1) was analysed by restriction mapping and southern hybridizations, and clones of ecori and sali fragments were prepared which represented approximately 85% of the genome. it has a length of about 135 kbp consisting of a long unique sequence joined to a short unique sequence bounded by inverted repeat sequences, and occurs as 2 isomers. these findings place mhv-1 in the herpesvirus structural classification group d, along with e ... | 1990 | 1964521 |
| antigenic and protein sequence homology between vp13/14, a herpes simplex virus type 1 tegument protein, and gp10, a glycoprotein of equine herpesvirus 1 and 4. | monospecific polyclonal antisera raised against vp13/14, a major tegument protein of herpes simplex virus type 1 cross-reacted with structural equine herpesvirus 1 and 4 proteins of mr 120,000 and 123,000, respectively; these proteins are identical in molecular weight to the corresponding glycoprotein 10 (gp10) of each virus. using a combination of immune precipitation and western immunoblotting techniques, we confirmed that anti-vp13/14 and a monoclonal antibody to gp10 reacted with the same pr ... | 1991 | 1850013 |
| properties and evolutionary relationships of the marek's disease virus homologues of protein kinase, glycoprotein d and glycoprotein i of herpes simplex virus. | the deduced amino acid sequences of the open reading frames (orfs) mapping in the short unique segment (us) of marek's disease virus (mdv) reported in the accompanying paper have been analysed using computer programs to determine their relationships to herpesvirus proteins. analysis of the catalytic domains of protein kinases showed that the mdv kinase (mdv pk) was closely related to the alphaherpesvirus protein kinase mapping in us. the results also showed that the mdv pk was more closely relat ... | 1991 | 1849976 |
| equine herpesvirus: new approaches to an old problem. | 1991 | 1849814 | |
| the conserved dna-binding domains encoded by the herpes simplex virus type 1 icp4, pseudorabies virus ie180, and varicella-zoster virus orf62 genes recognize similar sites in the corresponding promoters. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), pseudorabies virus (prv), varicella-zoster virus (vzv), and equine herpesvirus 1 (ehv-1) are all classified as alphaherpesvirinae. each of these five viruses encodes an essential immediate-early (ie) regulatory protein referred to as hsv-1 icp4, hsv-2 icp4, prv ie180, vzv orf62 protein, and ehv-1 ie1, respectively. these five proteins share extensive homology with each other in domains referred to as regions 2 and 4. the hsv-1 icp4 region 2 d ... | 1991 | 1847444 |
| the three major immediate-early transcripts of bovine herpesvirus 1 arise from two divergent and spliced transcription units. | among 54 transcripts expressed in a temporal cascade during lytic infection with bovine herpesvirus 1, we have previously identified three major immediate-early (ie) rnas, ier4.2 (4.2 kb), ier2.9 (2.9 kb), and ier1.7 (1.6 to 1.8 kb depending on the virus strain) transcribed from the hindiii c genome region (u. v. wirth, k. gunkel, m. engels, and m. schwyzer, j. virol. 63:4882-4889, 1989). northern (rna) blot, s1 nuclease protection, and primer extension analysis used in the present study demonst ... | 1991 | 1845884 |
| translational control of equine herpesvirus type 1 gene expression. | translational control mechanisms modulate gene expression in a variety of cellular and viral systems. using hypertonic conditions to block protein synthesis in vivo, we observed that the synthesis of several major equine herpesvirus type 1 proteins was selectively inhibited. although sensitivity to hypertonic conditions was graded across a continuum, messages coding for proteins of 203, 130.5, and 31.5 kda were significantly more resistant to higher salt concentrations in vivo than those coding ... | 1991 | 1845836 |
| sequence analysis of a glycoprotein d gene homolog within the unique short segment of the ehv-1 genome. | dna sequence analysis of one-third of the unique short (us) segment of the equine herpesvirus type 1 (ehv-1) genome revealed an open reading frame (orf) whose translated sequence exhibits significant homology to glycoprotein d of herpes simplex virus (hsv) types 1 and 2 and to pseudorabies virus (prv) glycoprotein 50, the gd equivalent. the orf of the ehv-1 gd homolog lies within the psz-4 bamhi/kpni fragment (map units 0.865 to 0.872 and 0.869 to 0.884) and is capable of encoding a polypeptide ... | 1991 | 1845821 |
| characterization of the major glycoproteins of equine herpesviruses 4 and 1 and asinine herpesvirus 3 using monoclonal antibodies. | a panel of 14 monoclonal antibodies (mabs) was used to characterize the high abundance glycoproteins of equine herpesviruses 4 (ehv-4) and 1 (ehv-1), and asinine herpesvirus 3 (ahv-3). the specificities of the mabs, which had been determined previously for strains of ehv-4 and -1 from the u.s.a., in general were confirmed by elisa for australian strains of these viruses. of the 14 mabs seven were ehv-4 and -1 type-common and cross-reacted with ahv-3. of the five mabs that were ehv-1 type-specifi ... | 1991 | 1716650 |
| studies on glycoprotein 13 (gp13) of equid herpesvirus 1 using affinity-purified gp13, glycoprotein-specific monoclonal antibodies and synthetic peptides in a hamster model. | hamsters were immunized with either an affinity-purified preparation of equid herpesvirus 1 (ehv-1) glycoprotein 13 (gp13) or synthetic peptides representing three sequences within the homologous glycoprotein of ehv-4, resulting in the production of anti-peptide (in the case of peptide-immunized animals) or antivirus antibodies. the sera from gp13-immunized hamsters contained antibodies which showed virus-neutralizing activity and complement-mediated antibody lysis of ehv-1-infected target cells ... | 1991 | 1707948 |
| evidence for allelic exclusion in chinese hamster ovary cells. | earlier results suggested that the functional hemizygosity of genes in pseudodiploid chinese hamster ovary (cho) cells is due to the silencing of one allele by dna methylation. from this one could make a strong prediction that we have now been able to confirm by genetic experiments, using thymidine kinase (tk) alleles. tk- mutants induced by ethylmethane sulphonate (ems) were all revertible to tk+ at high frequency by the demethylating agent 5-azacytidine (5-aza-cr). this revertibility was due t ... | 1990 | 1704254 |
| characterization of the gene and an antigenic determinant of equine herpesvirus type-1 glycoprotein 14 with homology to gb-equivalent glycoproteins of other herpesviruses. | the gene encoding glycoprotein 14 (gp14) of equine herpesvirus type 1 was sequenced. nucleotide sequence analysis revealed a complete transcription unit composed of a cat box, a tata box, a ribosome-binding sequence, a polyadenylation signal and an open reading frame (orf) of 2940 bp transcribed from left to right. the amino acid (aa) sequence deduced from this orf corresponded to that of a protein with 979 aa and had the characteristic features of membrane gp including a 20-aa signal sequence a ... | 1990 | 1692002 |
| amplification and differentiation of the dna of an abortigenic (type 1) and a respiratory (type 4) strain of equine herpesvirus by the polymerase chain reaction. | unpurified dna derived from cultures of equine fetal kidney cells infected with either equine herpesvirus type 1 or equine herpesvirus type 4 was amplified by the polymerase chain reaction using one pair of oligonucleotide primers. restriction endonuclease digestion of the amplified segments with pvuii, followed by electrophoresis, revealed restriction fragment length polymorphisms which enabled the two virus types to be differentiated. | 1991 | 1679247 |
| sequence characteristics of a gene in equine herpesvirus 1 homologous to glycoprotein h of herpes simplex virus. | a gene in equine herpesvirus 1 (ehv-1, equine abortion virus) homologous to the glycoprotein h gene of herpes simplex virus (hsv) was identified and characterised by its nucleotide and derived amino acid sequence. the ehv-1 gh gene is located at 0.47-0.49 map units and contains an open reading frame capable of specifying a polypeptide of 848 amino acids, including n- and c-terminal hydrophobic domains consistent with signal and membrane anchor regions respectively, and 11 potential sites for n-g ... | 1991 | 1666854 |
| identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein d gene. | a homologue of the herpes simplex virus (hsv) glycoprotein d gene has been identified in the genome of equine herpesvirus-1 (ehv-1, equine abortion virus). an open reading frame in the middle of the short unique (us) region is capable of encoding a polypeptide of 402 amino acids that has 26% and 20% of its residues matching pseudorabies virus (prv) gp50 and hsv-1 gd, respectively. despite this low level of similarity, the positional identity of six cysteine residues and certain motifs, and the l ... | 1991 | 1665613 |
| studies of bone marrow and leucocyte counts in peripheral blood in fetal and newborn foals. | clinical and pathological records of 124 foals were studied. the foals were assigned to six groups; normal, premature, dysmature, bacterially infected, neonatal maladjustment syndrome and equid herpesvirus type 1 (ehv-1) infected. also, 6 pony fetuses were sampled via catheters in the umbilical vein and artery between 280 and 310 days gestation. bone marrow aspiration was performed on a further 14 foals. premature foals had significantly lower neutrophil counts than normal foals up to 5 h. foals ... | 1991 | 1665520 |
| clinical signs and humoral immune response in horses following equine herpesvirus type-1 infection and their susceptibility to equine herpesvirus type-4 challenge. | a group of three horses was experimentally infected with equine herpesvirus type 1 (ehv-1) and showed clinical signs characterised by a biphasic febrile response, leucopenia and cell associated viraemia accompanied by virus shedding from the nasopharynx. a second exposure to the virus 18 days later resulted in the isolation of virus from the nasopharynx of one horse. this and a further group of three ehv-1 seropositive horses were subsequently infected with equine herpesvirus type 4 (ehv-4) 147 ... | 1991 | 1664967 |
| isolation of equine herpesvirus-1 mutants in the presence of (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine: demonstration of resistance in vitro and in vivo. | the compound (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine (hpmpa) had been previously shown to be highly effective in treatment of ehv-1 in a murine model for the equine disease. this paper describes the isolation of a series of mutants resistant to the drug. resistance was demonstrated in cell culture and one mutant was tested in a murine model. the resistant mutant was pathogenic for mice; infectious virus was recovered from respiratory tissues and blood at levels similar to the parental ... | 1991 | 1663728 |
| attempts to immunoprotect adult horses, specifically pregnant mares, with commercial vaccines against clinical disease induced by equine herpesvirus-1. | in a project lasting 4 years more than 300 lipizzans, around 180 of them adults, were vaccinated systematically against equine herpesvirus-1 (ehv-1) and representative groups thereof were serologically controlled for their antibody responses. in part, vaccination intervals recommended on packing slips were followed, in part other intervals, implicated by intermediary results, were used. a live virus vaccine proved ineffective if humoral antibodies were present. an oil-adjuvanted vaccine proved o ... | 1991 | 1659067 |
| location of open reading frames coding for equine herpesvirus type-1 glycoproteins with homology to ge and gi of herpes simplex virus. | the dna fragments representing the entire short unique region and part of the repeat sequences of the equine herpesvirus type-1 genome were cloned into plasmid vectors. the approximate positions of the junctions between the short unique region and the inverted repeats were then located by restriction endonuclease mapping. two open reading frames coding for potential glycoproteins have been identified within the short unique region, using dna sequence analysis. the predicted amino acid sequences ... | 1991 | 1656822 |
| a murine model for studying ehv-1-induced abortion. | balb/c mice were infected with two abortigenic strains of equine herpesvirus-1 (ehv-1) by intranasal inoculation. the inoculation of one strain produced subclinical disease while the other produced a disease characterised by weight loss, constitutional signs, and death in the most severely affected animals. when pregnant mice were infected by the same method of inoculation, one strain of virus produced premature parturition; both strains produced fetal abnormalities. in some cases, virus could b ... | 1991 | 1654586 |
| experimental equine herpesvirus-1 infection in llamas (lama glama). | three llamas (lama glama) were experimentally infected intranasally with an isolate of equine herpesvirus-1 (ehv-1) from the brain of an alpaca that had experienced severe neurologic signs. two of the 3 llamas developed severe neurologic disorders following inoculation; 1 died, and 1 was euthanized in a moribund state. the third llama showed only mild neurologic signs. the euthanized llama had preexisting antibodies to ehv-1, and the remaining 2 llamas were seronegative (virus neutralization tit ... | 1991 | 1654133 |
| the role of endothelial cell infection in the endometrium, placenta and foetus of equid herpesvirus 1 (ehv-1) abortions. | one of three mares in the last trimester of pregnancy became paraplegic 7 days after experimental infection with ehv-1 and was killed 10 days after infection (d.p.i.). the other two mares aborted foetuses at 12 and 14 d.p.i. in the first mare, virus was detected by immunofluorescence (iif) and immunoperoxidase (ip) staining in endothelial cells of the endometrium, placenta and umbilical vein, but not in any other foetal tissues. in the experimentally aborted foetuses, and in two other independen ... | 1991 | 1651960 |
| effect of tunicamycin and monensin on fusion and hemadsorptive activities of equid herpesvirus 1. | 1991 | 1650594 | |
| transcriptional analysis of the ul1 gene of equine herpesvirus 1: a gene conserved in the genome of defective interfering particles. | defective interfering particles (dips) of equine herpesvirus type 1 (ehv-1) are biologically active, in that they mediate the coestablishment of oncogenic transformation and persistent infection in permissive, primary hamster embryo fibroblasts. the dip genome is composed of ehv-1 sequences originating from the l-terminus (mapping units (m.u.) 0.00-0.023), the junction of the unique long (ul) region and the internal inverted repeat (ir) (m.u. 0.78-0.79 and 0.99-1.00), and the central portion of ... | 1991 | 1649513 |
| sequence analysis of the 4.7-kb bamhi-ecori fragment of the equine herpesvirus type-1 short unique region. | to localize gene that may encode immunogens potentially important for recombinant vaccine design, we have analysed a region of the equine herpesvirus type-1 (ehv-1) genome where a glycoprotein-encoding gene had previously been mapped. the 4707-bp bamhi-ecori fragment from the short unique region of the ehv-1 genome was sequenced. this sequence contains three entire open reading frames (orfs), and portions of two more. orf1 codes for 161 amino acids (aa), and represents the c terminus of a possib ... | 1991 | 1647359 |
| the epidemiology of equid herpesvirus abortion: a tantalizing mystery. | 1991 | 1646104 | |
| the raising of equine colostrum-deprived foals; maintenance and assessment of specific pathogen (ehv-1/4) free status. | over a period of two years, a total of 22 full term foals from welsh mountain pony mares were raised in conditions that were free from infection by equid herpesvirus (ehv-1/4). parturition dates were predicted by monitoring colostrum electrolytes, and the mares allowed to foal naturally under supervision or following induction with intravenous oxytocin. immediately following birth, foals were separated from their dams and transferred to a specially built, positive pressure isolation unit. they w ... | 1991 | 1646103 |
| an outbreak of equid herpesvirus abortion in new south wales. | thirty-three of the 44 mares on a thoroughbred stud in new south wales aborted or lost foals within one day of birth. gross pathological and histological changes were in keeping with equid herpesvirus i (ehv-1) abortion. in the six foals that underwent virological examination, ehv was isolated and typed as ehv-1 by restriction endonuclease analysis. ehv-1 abortion had not occurred previously on this stud and the source of the infection was not identified. | 1991 | 1646102 |
| [year-round antibody profile of groups of horses of a herd kept in isolation after differently terminating use of an experimental viral combination vaccine]. | the commercial vaccine "resequin f konz." devised against viral respiratory infections of horses contains the abortigenic equine herpesvirus-1 (ehv-1). therefore we had used it in our protection project of the austrian lipizzaners+ primarily to prevent abortions. taking into account the recent perception that for young horses the respiratory-pathogenic ehv-4 type is essential behringwerke marburg added this particular virus to their market product to produce a multicomponent experimental vaccine ... | 1991 | 1646100 |
| equine herpesvirus 1 sequence near the left terminus codes for two open reading frames. | we have previously reported the sequence of the equine herpesvirus one genomic termini that are homologous to the genomic termini of other herpesviruses. in this paper, we present the nucleotide sequence adjacent to the left terminus sequence (map units 0.0087 to 0.0237). this sequence codes for two open reading frames (orf) which are homologous to orf2 and orf3 of the varicella-zoster virus genome and are located at colinear positions. the l region sequence presented here also contains a segmen ... | 1991 | 1645901 |
| an early gene maps within and is 3' coterminal with the immediate-early gene of equine herpesvirus 1. | the immediate-early (ie) gene (ir1 gene) of equine herpesvirus 1 (ehv-1) encodes a single, spliced 6.0-kb mrna during cytolytic infection. however, under early (in the presence of phosphonoacetic acid) and late (8 h postinfection; no metabolic inhibitors) conditions, in addition to the 6.0-kb ie mrna, a 4.4-kb early (e) mrna is transcribed from the ie gene region beginning at approximately 4 h postinfection. to map and characterize the 4.4-kb e mrna and the protein product of this early gene (ir ... | 1991 | 1645793 |
| [virologico-serologic studies in horses with respiratory tract diseases]. | of 1081 acute and chronically respiratory diseased as well as clinically normal horses 824 sera and 257 paired serum samples collected 1986 and 1987 were tested for antibodies against several different respiratory viruses such as influenza virus a/equi 1 and 2 (influenza 1 a. 2), equine herpesvirus type 1/4 (ehv 1/4), mammalian reovirus type 1-3 (reovirus 1-3), equine rhinovirus type 1 (erv 1), equine adenovirus type 1 (eadv 1), and equine arteritis virus (eav). the investigations resulted in an ... | 1992 | 1558530 |
| identification and control of the cis-acting elements of the immediate early gene of equid herpesvirus type 1. | consensus cis-acting dna sequences upstream of the immediate early (ie) gene of equid herpesvirus type 1 (ehv-1, strain ab4) were identified. one copy of the conserved motif taatgarattc, which is the binding site for the host cellular factor oct-1 and herpes simplex virus type 1 (hsv-1) virion protein, vmw65, complex, was identified at positions -630 to -620. using transient transfections and chloramphenicol acetyltransferase assays the ie promoter of ehv-1 was shown to be trans-activated by vmw ... | 1992 | 1545217 |
| identification of equine herpesvirus 4 glycoprotein g: a type-specific, secreted glycoprotein. | equine herpesvirus 4 (ehv4) glycoproteins of m(r) 63k and 250k were identified in the supernatant of infected cell cultures. the 63k glycoprotein was type-specific; that is, it reacted with monospecific sera from horses that had been immunized or infected with ehv4, but not with monospecific sera from horses immunized or infected with ehv1, a closely related alphaherpesvirus. it was postulated that the secreted protein may be the homologue of similarly secreted glycoproteins of herpes simplex vi ... | 1992 | 1529525 |
| [causes of prenatal foal loss in switzerland]. | in switzerland during the foaling season 1988 and 1989 the cause of abortion in 60 foals was investigated. special attention was paid to infections with equine herpesvirus 1 (ehv 1). diagnosis were based on post-mortem, histopathological, bacteriological and immunofluorescence investigation. the results confirm data from other countries, that ehv 1 is the most prevalent viral (20%) cause of abortion, followed by various bacterial agents (12%). other causes were umbilical torsion, twin pregnancy ... | 1992 | 1455212 |
| equine herpesvirus 1 glycoprotein d: mapping of the transcript and a neutralization epitope. | studies with molecular and immunological techniques identified and mapped the transcript encoding glycoprotein d (gd) of equine herpesvirus 1 kya, as well as two continuous gd antigenic determinants. three mrna species of 5.5, 3.8, and 1.7 kb overlap the gd open reading frame and are transcribed from the dna strand encoding gd. northern (rna) blot hybridization with both dna clones and riboprobes, as well as s1 nuclease analyses, showed the 3.8-kb mrna to encode gd and to be synthesized as a lat ... | 1992 | 1383565 |
| icp22 homolog of equine herpesvirus 1: expression from early and late promoters. | the complete nucleotide sequence of the short region, made up of a unique segment (us; 6.5 kb) bracketed by a pair of inverted repeat sequences (ir; 12.8 kb each), of the equine herpesvirus 1 (ehv-1) genome has been determined recently in our laboratory. analysis of the ir segment revealed a major open reading frame (orf) designated ir4. the ir4 orf exhibits significant homology to the immediate-early gene us1 (icp22) of herpes simplex virus type 1 and to the icp22 homologs of varicella-zoster v ... | 1992 | 1370553 |
| latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues. | the polymerase chain reaction (pcr) and co-cultivation were used to identify the lymphoreticular system as the site of latency of equid herpesvirus i (ehv-1). primers for pcr were designed from aligned nucleotide sequences of the glycoprotein gb genes to amplify the same region of both the ehv-1 and ehv-4 genomes. subsequent restriction digests using specific enzymes distinguished the amplified fragments of the ehv-1 genome from those of the ehv-4 genome. ten weeks following an experimental infe ... | 1992 | 1347078 |
| detection of equine herpesvirus and differentiation of equine herpesvirus type 1 from type 4 by the polymerase chain reaction. | although both equine herpesvirus type 1 (ehv-1) and equine herpesvirus type 4 (ehv-4) can be associated with respiratory disease, epizootics caused by ehv-1 are much more serious because the virus can cause abortions and paralysis. it is, therefore, important to identify the type of ehv involved in an outbreak by a test that is quick, sensitive, and reliable. we have adapted the polymerase chain reaction (pcr) to detect and distinguish between ehv-1 and ehv-4 in the same reaction. primers for pc ... | 1992 | 1335829 |
| immunokinetics of equine herpesvirus 1 in donkey mares: suppression of secondary cell-mediated response. | to study the immunokinetics of equine herpesvirus 1 (ehv1), donkey mares were immunised with a laboratory strain of ehv1, or with recommended doses of pneumabort-k vaccine (ehv1 army 183 strain, formalin-inactivated, with an oil adjuvant) and a booster was given after three months. humoral immune responses were studied by employing a virus neutralisation (vn) test. a leucocyte migration inhibition test (lmit) was employed for the assay of cellular immune responses. the vn antibody titre reached ... | 1992 | 1335311 |
| the ir3 gene of equine herpesvirus type 1: a unique gene regulated by sequences within the intron of the immediate-early gene. | the complete nucleotide sequence of the inverted repeat component (ir; 12,776 bp each) of the genome of equine herpesvirus type 1 (ehv-1) has been determined. transcription analyses have revealed that the ehv-1 ir sequence encodes at least 6 genes. in this report, we present the dna sequence and transcriptional characterization of a gene (ir3) that maps entirely within the ir sequences. the ir3 open reading frame (orf) is located between nucleotides (nt) 6123-6411 of the ir sequence and possesse ... | 1992 | 1335300 |
| serological evidence of equine herpesvirus type 1 (ehv-1) activity in polo horses in nigeria. | serological evidence of equine herpes virus type 1 (ehv-1) activity in polo horses in nigeria is reported for the first time. eighty-two percent of horses tested with known antigen had precipitating antibodies to ehv-1 while 43% of sera tested against antigen prepared from nasal discharges were positive suggesting that the virus was being excreted in the nasal discharges and probably acting as a source of infection for incontact animals as occurs in on-going acute infections. the result of this ... | 1992 | 1334303 |
| serological responses of specific pathogen-free foals to equine herpesvirus-1: primary and secondary infection, and reactivation. | serum antibody (virus neutralisation, complement fixation, igm and igg) responses to equine herpesvirus-1 (ehv-1) infection were measured in six foals which were initially free from ehv-1 and ehv-4 infection and maternally-derived antibodies. following primary infection, high titres of virus neutralisation and complement fixation antibodies were detectable against ehv-1, however, corresponding antibody levels against ehv-4 were low or inapparent, although the two viruses share a number of cross- ... | 1992 | 1333670 |
| identification and transcriptional mapping of genes encoded at the ir/us junction of equine herpesvirus type 1. | two open reading frames (orfs) encoded at the inverted repeat unique short (us) junction of the short (s) region of the equine herpesvirus type 1 genome were identified by dna sequencing of a 2876 base pair (bp) genomic segment, and transcripts encoding these orfs were characterized by northern blot, s1 nuclease, and primer extension analyses. these studies also established the size of each inverted repeat to be 12,768 nucleotides (nts). the ir6 orf (816 bp), mapping at nts 12,317-11,502 of the ... | 1992 | 1333117 |
| the activity of (s)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (hpmpc) against equine herpesvirus-1 (ehv-1) in cell cultures, mice and horses. | the activity of the nucleotide analogue, (s)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (hpmpc), against equine herpesvirus-1 (ehv-1) was tested in cell culture, mice and foals. the ed50 for plaque reduction was found to be 0.07 and 0.03 microgram/ml in rk-13 and eel cells respectively. in mice, a single administration of hpmpc (20 mg/kg, s.c.) was very effective at reducing clinical signs and virus replication if given on the day before intranasal inoculation with ehv-1. treatment on ... | 1992 | 1332605 |
| chorioretinopathy associated with neuropathology following infection with equine herpesvirus-1. | 1992 | 1332242 | |
| glycoprotein 300 is encoded by gene 28 of equine herpesvirus type 1: a new family of herpesvirus membrane proteins? | a portion of equine herpesvirus type 1 (ehv-1) gene 28, which is homologous to herpes simplex virus type 1 gene ul32, was expressed using a prokaryotic system to yield a fusion protein which reacted on western blots with p19, a monoclonal antibody (mab) that reacts with ehv-1 glycoprotein 300 (gp300), confirming that this gene encodes gp300. hydrophobicity analysis showed that gp300 is a glycoprotein with multiple hydrophobic domains that might interact with, or span, the membrane several times. ... | 1992 | 1331295 |
| rapid detection of equine herpesvirus type-1 antigens in nasal swab specimens using an antigen capture enzyme-linked immunosorbent assay. | an antigen capture enzyme-linked immunosorbent assay (elisa) was developed for the detection of equine herpesvirus type-1 (ehv-1) antigens in nasal swab specimens. the test was designed as a solid phase, amplified sandwich assay in which an ehv-1 specific monoclonal antibody was used to capture virus antigen and polyclonal antisera used to detect antigen bound to the test plates. eight monoclonal antibodies were tested for their ability to capture virus antigen and one was selected for routine u ... | 1992 | 1331153 |
| the acyclic nucleoside analogue penciclovir is a potent inhibitor of equine herpesvirus type 1 (ehv-1) in tissue culture and in a murine model. | equine herpesvirus type 1 (ehv-1) was sensitive to the nucleoside analogue penciclovir (pcv) when tested in tissue culture; the ed50 was 1.6 micrograms/ml. drug-resistant mutants were selected which were found to be tk-defective and approx. 45-fold less sensitive to pcv compared with the parental strain. pcv was compared with the phosphonyl derivative, hpmpa in mice infected with ehv-1. both drugs were shown to be effective in vivo, limiting wild-type virus replication in respiratory tissues, an ... | 1992 | 1329646 |
| identification and expression of the ul1 gene product of equine herpesvirus 1. | sequences encoding the ul1 gene of equine herpesvirus type 1 (ehv-1) are conserved in the genome of ehv-1 defective interfering particles (dips) that mediate oncogenic transformation and persistent infection. the ul1 protein was identified by in vitro transcription/translation and hybrid-arrest translation analyses which employed a ul1/pgem-3z construct designated pgeml1. sds-page analyses of in vitro translation products synthesized from ul1-specific rna revealed that the ul1 orf encodes a 30 k ... | 1992 | 1329372 |
| the equine herpesvirus type 1 (ehv-1) homolog of herpes simplex virus type 1 us9 and the nature of a major deletion within the unique short segment of the ehv-1 kya strain genome. | the dna sequence of the short (s) genomic component of the equine herpesvirus type 1 (ehv-1)kya strain has been determined recently in our laboratory. analysis of a 1353-bp bamhi/pvuii clone mapping at the unique short/terminal inverted repeat (us/tr) junction revealed 507 bp of us and 846 bp of tr sequences as well as an open reading frame (orf) that is contained entirely within the us. this orf encodes a potential polypeptide of 219 amino acids that shows significant homology to the us9 protei ... | 1992 | 1326805 |
| vaccination of mares against equine herpesvirus-1. | 1992 | 1324543 | |
| identification and transcriptional analyses of the ul3 and ul4 genes of equine herpesvirus 1, homologs of the icp27 and glycoprotein k genes of herpes simplex virus. | the dna sequence of 3,240 nucleotides of the xbai g fragment located in the unique long (ul) region of the equine herpesvirus 1 genome revealed two major open reading frames (orfs) designated ul3 and ul4. the ul3 orf of 470 amino acids (aa) maps at nucleotides (nt) 4450 to 3038 from the long terminus, and its predicted 51.4-kda protein product exhibits significant homology to the icp27 alpha regulatory protein of herpes simplex virus type 1 (hsv-1; 32% identity) and to the orf4 protein of varice ... | 1992 | 1323700 |