TitleAbstractYear(sorted ascending)
fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein d.the fine structure of the antigenic determinants of herpes simplex virus type 1 and 2 glycoprotein d (gd) was analyzed to determine whether structural differences underlie the differential immunogenicity of these glycoproteins. a region common to herpes simplex virus type 1 and 2 gd (amino acid residues 11 to 19) and two sites specific for herpes simplex virus type 2 gd (one determined by proline at position 7, the other determined by asparagine at position 21) were localized within the n-termin ...19846208376
sequence of the putative origin of replication in the ul region of herpes simplex virus type 1 ang dna.interest has been stimulated concerning the region mapping between 0.38 and 0.42 on the prototype configuration of the herpes simplex virus type 1 (hsv-1) genome due to the high probability of the presence there of a second origin of dna replication. a 960 bp restriction fragment (hinfi e) of a class ii defective hsv-1 ang dna has been sequenced using the viral dna rather than molecularly cloned dna. this fragment includes the bamhi u/r cleavage site, mapping at approximately 0.4. part of the se ...19846096489
pathogenesis of genital herpes simplex virus infection in mice. iii. comparison of the virulence of wild and mutant strains.this study was undertaken to establish the role of virulence of various herpes simplex virus (hsv) strains in the course of infection when applying the virus to the non-injured mucous membranes of mice. wild-type hsv-type 1 (hsv-1) strains with marked differences in their neurovirulence following intracerebral inoculation showed minor differences in virulence after vaginal inoculation, but essentially their neurovirulence in cerebral infection corresponded to their virulence on the mucous membra ...19846096684
metabolic fate of (e)-5-(2-bromovinyl)-2'-deoxyuridine in herpes simplex virus- and mock-infected cells.(e)-5-(2-bromovinyl)-2'-deoxyuridine is a potent antiherpes compound with far better activity against herpes simplex virus type 1 than type 2. to understand the role of drug metabolism in this differential antiviral activity, we examined the metabolic fate of this drug in virus-infected and mock-infected vero cells by high-pressure liquid chromatography. after 8 h of incubation in which cells were exposed to 10 micrograms of the drug per ml, 63 pmol/10(6) cells of the parent compound was detecte ...19846097176
alteration of lymphocyte transformation response to herpes simplex virus infection by acyclovir evaluate the effect of acyclovir (acv) therapy on the cellular immune response, we sequentially followed 43 patients with culture-proven first episodes of genital herpes simplex virus (hsv) infection. twenty-three patients who were treated with acv and 20 who received placebo had blood obtained weekly during the first 6 weeks after onset of lesions and had their in vitro lymphocyte transformation (lt) response to inactivated hsv antigens measured. the mean stimulation index to hsv antigens at ...19846098221
monoclonal antibodies against herpes simplex viruses.hybridoma cell lines secreting monoclonal antibodies against hsv-1 and 2 have been produced. balb/c mice were immunized by footpad infection and boosted by i.v. injection of viral antigens extracted from virus infected balb/c fibroblasts. after fusion hybrid cells were screened for antibody production by elisa and indirect immunofluorescence (iif). in our hands, iif had similar sensitivity and higher specificity than elisa but was more time-consuming. selected hybridomas have been characterized ...19846098497
[studies of the nature of tubular structures which appear in herpes simplex virus type 2-infected cells. studies with temperature-sensitive mutants]. 19846099328
use of aggregating brain cultures to study the replication of herpes simplex virus types 1 and 2 in central nervous system tissue.a novel tissue culture system consisting of reaggregated embryonic mouse brain cells was used to examine the replication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) within central nervous system tissue. brain aggregates cultured 30-40 days in vitro demonstrated progressive maturation and differentiation into cells recognizable as neurons, astrocytes and oligodendrocytes, with the latter cell type exhibiting myelin production. mature aggregates were infected with hsv and sampled at ...19846099372
herpes simplex virus genital infection of the female guinea pig as a model for the evaluation of an experimental vaccine.substantial protection against herpes simplex type 2 (hsv 2) infection of the female guinea pig genital tract was provided by immunization with an experimental hsv 2 vaccine prepared from the plasma membranes of infected mrc-5 cells. protection was evaluated in terms of the modification of histopathological lesions and clinical signs and in changes in viral replication and serological responses in vaccinated and control animals.19846099646
antibodies to membrane antigens of herpes simplex virus type-2 infected cells and hsv-2 specific antibodies in patients with cervical cancer in a geographical study involving the united kingdom, sudan, sri lanka and malawi, sera were taken from 27 malawian patients with cervical cancer and 18 controls matched for age, ethnic origin and socioeconomic status. the sera were tested by indirect immunofluorescence for igg and iga antibodies to membrane antigens of herpes simplex virus type-2 (hsv-2). the results showed that there was no significant difference at titres 1:4 between malawian patients and controls, but at titres 1:16 the diff ...19846100279
role of cytomegalovirus in kaposi's sarcoma.previous studies have established an association between cytomegalovirus (cmv) and classic and endemic kaposi's sarcoma (ks) which can be extended to include the epidemic form of ks. the identification of nucleic acid sequences homologous to cmv but not to epstein-barr virus (ebv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) as well as the detection of cmv gene products, particularly early antigens, in tumour biopsies and/or early cultures derived from them is an important criterio ...19846100282
herpes simplex virus type 2 and cervical cancer. 19846100718
demonstration of a herpes simplex virus type 2-induced deoxyuridine triphosphate nucleotidohydrolase in infected kb cells and in biochemically transformed hela cells.the deoxyuridine triphosphate nucleotidohydrolase (dutpase) activity which is induced in kb cells infected with herpes simplex virus type 2 (hsv-2) and expressed in biochemically transformed hela cells was separated from the host dutpase activity using either gel filtration or chromatofocusing chromatography. the hsv-2-induced dutpase differed from the host dutpase activity in its molecular weight (53000 versus 46000) and isoelectric point (pi 5 x 9 versus 6 x 4). however, it was similar to the ...19846141216
antiherpes activity of some novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (e-brvudr) in two different cell a series of 5-vinyl-2'-deoxyuridine (vudr) analogues (5-(2-x-vinyl)-udrs) the (e)-5-(2-bromovinyl)-udr (e-brvudr) proved the most potent inhibitor of plaque formation of two herpes simplex virus type 1 (hsv-1) strains in human embryonic lung fibroblast (helf) and african green monkey kidney (vero) cell cultures. the (z)-5-(2-bromovinyl)-udr (z-brvudr) isomer and the 5-(2,2-dibromovinyl)-udr (br2vudr) analogue were 10-20 times less efficient, whereas the (e)-5-(2-cyanovinyl)-udr (cnvudr) and t ...19846143491
effects of herpes simplex virus strains on human fibroblast and lymphocyte chromosomes and the localization of chromosomal aberrations.the effects on human chromosomes of types 1, 2 and of intermediate serotype of herpes simplex virus (hsv) was compared in fibroblast and lymphocyte cultures. the karyological changes due to hsv were shown to depend on the serotype used as well as on the kind of cells examined (agent-specificity and cell-reaction specificity). differences were noted among the strains in relation to the degree and character of the aberrations induced. conventional giemsa staining and the trypsin g-banding techniqu ...19846145351
differential antiherpes activity of the (e)-and (z)-isomers of 5-(2-fluorovinyl)-2'-deoxyuridine (fvudr).four novel analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine [(e)-brvudr]--the (e)- and (z)-isomer of 5-(2-fluorovinyl)-udr (fvudr), (z)-5-(2-carboxy-2-fluorovinyl)-udr [(z)-cooh-fvudr], and (e)-5-(2-ethoxyvinyl)-udr [(e)-eovudr] were compared with the reference compounds (e)-brvudr and 5-vinyl-udr (vudr) for their inhibitory effects on plaque formation of herpes simplex virus type 1 (hsv-1 strain 77) and type 2 (hsv-2 strain 82) in human embryonic lung fibroblast (helf) cell cultures. (z)-fvudr ...19846148852
purification of herpes simplex virus-specified major dna-binding proteins and detection of antibodies to them in human and rabbit sera.we purified herpes simplex virus type 1 and 2-specified nonstructural dna-binding proteins (icp8 and icsp 11/12, respectively) from infected vero cells and applied them in elisa test to analyse human and rabbit sera. only a weak immune response to the icp8 and icsp 11/12 could be demonstrated following experimental hsv-1 or hsv-2 infections of rabbits. low levels of icsp8 antibodies were found also in acute hsv infections of man.19846152122
comparative activities of selected combinations of acyclovir, vidarabine, arabinosyl hypoxanthine, interferon, and polyriboinosinic acid-polyribocytidylic acid complex against herpes simplex virus type 2 in tissue culture and intravaginally inoculated mice.the effects of double and triple combinations of acyclovir (acv), adenine arabinoside (ara-a), arabinosyl hypoxanthine, or interferon on herpes simplex virus type 2 in mouse embryo fibroblasts were measured. these in vitro data were compared with results obtained in mice infected intravaginally with herpes simplex virus type 2 and treated intraperitoneally with low- and high-dose combinations of acv, ara-a, or polyriboinosinic-polyribocytidylic acid(poly-l-lysine)carboxymethylcellulose complex [ ...19846083754
[detection of the virus-specific antigens of adenoviruses and herpes simplex virus in patients with malignant genital diseases].the possibility of detecting virus-specific antigens in women with various pathological changes of the genitalia (cancer and pre-cancer conditions) was demonstrated. comparative examinations of specimens from patients with cancer diseases of the genitalia for the presence of structural and virus-induced early antigens of hsv-2 demonstrated immunofluorescence test (ift) to be the diagnostic test for the detection of hsv antigens in patients with cervical carcinoma. the rate of virus-induced antig ...19846084901
prevalence of antibodies to herpes simplex virus and frequency of hla antigens in patients with preinvasive and invasive cervical cancer.previous herpes simplex virus type 2 (hsv-2) infections are known to predispose women for the development of cervical cancer, but causal relationship between the virus and the cancer has never been proven. forty-six patients with cervical carcinoma (13 with preinvasive lesions and 33 with various stages of invasive disease) were selected for the present study. among the patients 96% were seropositive for the presence of antibodies to herpes simplex virus compared to 87% of the 30 controls. antib ...19846086470
human lymphoblastoid cell lines established from peripheral blood lymphocytes secreting immunoglobulins directed against herpes simplex virus.peripheral blood lymphocytes (pbl) from genital herpes simplex virus type 2 (hsv-2) patients were transformed with epstein-barr virus. using conditions optimized for successful transformation, two lymphoblastoid cell lines (lcl) have been established, which secrete immunoglobulin g (igg) to hsv. these results suggest that pbl from patients suffering recurrent herpes virus infections can be used to establish lcl secreting hsv antigen specific human immunoglobulins.19846086505
rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay.a sensitive enzyme-linked immunosorbent capture assay with biotin and streptavidin (capture b/sa elisa) was developed to detect herpes simplex virus (hsv) antigen. rabbit anti-hsv antibody (immunoglobulin g fraction) was coated on flat-bottom, irradiated, 96-well polystyrene microtiter plates and served to capture hsv antigen. clinical specimens from patients with genital herpes were added. biotin-linked rabbit anti-hsv immunoglobulin g was used as the second antibody. the antigen-antibody compl ...19846086705
antibodies to a synthetic oligopeptide that react with herpes simplex virus type 1 and 2 glycoprotein c.nucleotide sequence and mrna localization studies have allowed the prediction of the amino acid sequence of herpes simplex virus type 1 (hsv-1) glycoprotein c (gc). we immunized a rabbit with a conjugate of bovine serum albumin and a synthetic peptide having the same sequence as that deduced for amino acids 128 through 139 of hsv-1 gc. a very similar amino acid sequence has been predicted to exist in the related product, herpes simplex virus type 2 (hsv-2) gc, which was formerly designated gf. p ...19846086954
small fragments of herpesvirus dna with transforming activity contain insertion sequence-like structures.a 737-base-pair fragment of herpes simplex virus type 2 dna with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the bgl ii n fragment region (map position 0.58-0.625), which were constructed in vitro. the transforming sequences lie within the coding region for a mr 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. contained within the transforming fragment are sequences that can b ...19846087345
reliable identification of herpes simplex viruses by dna restriction endonuclease analysis with ecori.a simple procedure that permits the rapid identification of clinical isolates of herpes simplex virus (hsv, type 1 or type 2) on the basis of stable and type-specific differences in the ecori restriction endonuclease patterns of the hsv-1 and hsv-2 genomes was tested for reliability. complete concordance was first obtained for 298 hsv isolates typed by standard laboratory procedures and by analysis of the dna profiles. two other hsv isolates showing intermediate biologic, immunologic, and/or bio ...19846087480
inapparent genital herpes simplex infection in women attending a venereal disease clinic.the purpose of this study was to determine the prevalence of asymptomatic genital infection with herpes simplex virus in women attending a venereal disease clinic in puerto rico. genital samples were obtained from 123 consecutively enrolled patients. vero cells were used for viral isolation; isolates demonstrating cytopathic effect were stored at -70 c and were typed by use of monoclonal antibodies. herpes simplex virus was isolated from six patients, three of whom were free of any possible comp ...19846087481
herpes simplex virus transmission: condom studies.the incidence of genital herpes simplex infections has risen at a rapid rate over the past 20 years. sexually active young adults ask whether condoms will effectively block the transmission of herpes simplex virus from one individual to another. using a laboratory plunger model to stimulate human coitus, the authors have shown that high concentrations of hsv-2 did not cross the latex or natural membrane condoms that were tested. we have also shown that high concentrations of hsv-2 left in contac ...19846087482
herpes simplex virus type-2 glycoprotein-subunit vaccine: tolerance and humoral and cellular responses in humans.twenty-three subjects were administered three doses of inactivated herpes simplex virus (hsv) type 2 (hsv-2) glycoprotein-subunit vaccine, with doses 2 and 3 being given four and 22 weeks after dose 1. both hsv-neutralizing antibody and antibody effective in antibody-dependent cell-mediated cytotoxicity were detected in serum samples of 12 of the 13 initially seronegative subjects by week 8. the geometric mean titers of both antibody types decreased between weeks 8 and 22 but increased one week ...19846088648
immunity in the female genital tract after intravaginal vaccination of mice with an attenuated strain of herpes simplex virus type 2.herpes simplex virus type 2 is a common human venereal pathogen which causes lethal neurological illness after intravaginal inoculation into balb/cj mice. to investigate whether an attenuated, nonlethal strain of this virus would confer immunity after inoculation of mice, we constructed a strain containing a partial deletion of the thymidine kinase gene, which is necessary for viral replication and spread in sensory ganglia. unlike its wild-type counterpart, this deletion-containing strain of he ...19846088797
rapid identification of cloned hsv2 dna fragments.randomly generated herpes simplex virus type 2 (hsv2) dna fragments can be localized on the viral genome by digestion with smai and subsequent comparison of the resulting fragment patterns with a reference picture of smai-digested recombinant plasmids that contain inserts representing the entire hsv2 (strain 333) genome as adjacent fragments. the feasibility of the technique, which we call 'sma fingerprinting' and which is much faster than fragment identification by blot hybridization, is demons ...19846090338
the immunocytochemical detection of herpes simplex virus in cervical smears--a valuable technique for routine use.herpes simplex virus type 2 is demonstrated in routine cervical smears by immunocytochemistry. optimum antibody dilution and suitable fixation were determined and immunostaining results were found to compare favourably with those obtained by screening papanicolaou-stained smears or by virus culture methods (virus isolation). results obtained for immunostaining of smears from 158 patients agreed with those of virus isolation in 47 of 50 cases where virus isolation was performed. it is suggested t ...19846090625
characterization of herpes simplex virus 1 alpha proteins 0, 4, and 27 with monoclonal antibodies.analyses of the reactivity and patterns of synthesis of infected cell polypeptides (icps) specified by herpes simplex virus (hsv) 1 and 2 and by hsv-1 x hsv-2 recombinants indicated that monoclonal antibody h1183 reacted with hsv-1 alpha icp0, whereas monoclonal antibody h1113 reacted with both hsv-1 and hsv-2 alpha icp27. h1083 and h1113 and a monoclonal antibody to icp4 (h640) similar to one previously described (d. k. braun et al., j. virol. 46:103-112.) were then used to study the properties ...19846090689
extensive homology between the herpes simplex virus type 2 glycoprotein f gene and the herpes simplex virus type 1 glycoprotein c gene.the region of the herpes simplex virus type 2 (hsv-2) genome which maps colinearly with the hsv-1 glycoprotein c (gc) gene has been cloned, and the dna sequence of a 2.29-kilobase region has been determined. contained within this sequence is a major open reading frame of 479 amino acids. the carboxyterminal three-fourths of the derived hsv-2 protein sequence showed a high degree of sequence homology to the hsv-1 gc amino acid sequence reported by frink et al. (j. virol. 45:634-647, 1983). the am ...19846090692
complement-requiring neutralizing antibody in guinea pigs with primary and recurrent genital herpes.guinea pigs inoculated intravaginally with herpes simplex virus type 2 (hsv-2) strain 1868 produced a serum complement-requiring neutralizing (crn) antibody during primary acute infection, i.e., 10 days postinoculation. the crn antibody titers in the guinea pig sera decreased to less than 1:10 after heating at 56 degrees c for 30 min. it was found that 32 units of complement were necessary to obtain a satisfactory hsv-2 neutralizing antibody titer. nonheated sera significantly reduced virus infe ...19846091148
more rapid isolation of herpes simplex virus in a continuous line of mink lung cells than in vero or human fibroblast cells.herpes simplex virus (hsv) was isolated from clinical specimens more rapidly in mink lung (ml) cells, a continuous cell line available from a commercial supplier, than in vero cells or human fibroblast (hf) cells. stock strains of hsv type 1 (hsv-1) and hsv-2 titered higher in ml cells than in vero or hf cells. ml cells were equivalent to rabbit kidney (rk) cells in the isolation of hsv in clinical specimens, but titers of stock hsv strains were lower. ml cells could be employed to type strains ...19846091988
the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine.the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ...19846092320
expression of recombinant genes containing herpes simplex virus delayed-early and immediate-early regulatory regions and trans activation by herpesvirus infection.the promoter-regulatory regions from the herpes simplex virus type 1 (hsv-1) gene for the immediate-early, 175,000-molecular-weight (175k) protein and the hsv-2 delayed-early gene for a 38k protein were linked to the readily assayable bacterial gene for the enzyme chloramphenicol acetyltransferase (cat). unexpectedly, in measurements of the constitutive expression of the recombinant genes 40 to 50 h after transfection of vero cells, enzyme levels expressed from the delayed-early 38k-promoter-cat ...19846092673
herpes simplex virus types 1 and 2 homology in the region between 0.58 and 0.68 map units.the homology between herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2, respectively) dna between 0.58 and 0.674 map units was compared by southern and dot blot analysis with dna of one type of virus as a hybridization probe against the other type. regions of high homology were interspersed with regions of detectably lower homology. however, only one region (between 0.647 and 0.653 map units) contained few or no homologous sequences. in situ rna blot hybridization demonstrated that the mrn ...19846092683
ribonucleotide reductase of herpes simplex virus type 2 resembles that of herpes simplex virus type 1.the ribonucleotide reductase (ribonucleoside-diphosphate reductase; ec induced by herpes simplex virus type 2 infection of serum-starved bhk-21 cells was purified to provide a preparation practically free of both eucaryotic ribonucleotide reductase and contaminating enzymes that could significantly deplete the substrates. certain key properties of the herpes simplex virus type 2 ribonucleotide reductase were examined to define the extent to which it resembled the herpes simplex virus t ...19846092726
the nervous system in genital herpes simplex virus type 2 infections in mice. lethal panmyelitis or nonlethal demyelinative myelitis or meningitis.female mice were inoculated vaginally with the ms strain of herpes simplex virus type 2, and serially positive vaginal cultures were used to confirm infection. the proportion of mice infected and the mortality rate in infected mice decreased with increasing age. in mice 12 weeks old, clinical, neuropathologic, and virologic criteria defined four patterns of disease. moribund mice had severe genital lesions, hindleg paralysis, and urinary and fecal retention, and most died during the second week ...19846092778
characterisation of a herpes simplex virus type 1 mutant which has a temperature-sensitive defect in penetration of cells and assembly of capsids.a herpes simplex virus type 1 (hsv-1) mutant, ts1204, which has a temperature-sensitive (ts) mutation located within genome map coordinates 0.318 to 0.324, close to but outside the coding sequences of the glycoprotein gb gene, has been characterised. although this mutant adsorbed to the cell surface at the nonpermissive temperature (npt), it failed to penetrate the cell membrane. as a consequence of this defect, high multiplicities of infection of ts1204 blocked subsequent infection of cells by ...19846093367
interactions between herpes simplex virus dna-binding proteins.monoclonal antibodies directed against several herpes simplex virus (hsv)-induced dna-binding proteins were used to investigate protein interactions in hsv-infected cells. q1 monoclonal antibody, which is specific for the hsv-induced alkaline nuclease, when used in an immunoadsorbant column resulted in the purification of the alkaline nuclease, to which large quantities of the major dna-binding protein were bound. conversely, when a monoclonal antibody to the major dna-binding protein was used i ...19846094715
infection of murine hepatocyte cultures by herpes simplex virus (hsv) 1 and 2.a study was undertaken of the interaction between liver cells and herpes simplex virus (hsv) in vitro. hepatocytes were obtained from hsv-resistant (c57/b16) and from hsv-susceptible (balb/c, a/j, c3h) mouse strains and cultured according to standard methods. each culture was infected with several strains of hsv-type 1 or of hsv-type 2, respectively. the multiplicity of infection was 5. the cytopathic effect was evaluated by light- and electron-microscopy. the number of infectious particles was ...19846094943
herpes simplex virus latency in immunosuppressed determine the role of antibodies in establishing herpes simplex virus (hsv) latency, immunosuppressed swiss mice were experimentally infected in the right hind footpad with a hsv-1 x hsv-2 recombinant (c6d) with low virulence. immunosuppression was induced by repeated intraperitoneal inoculations of cyclophosphamide (cy) and the production of antibodies to c6d in immunosuppressed animals was monitored by enzyme linked immunosorbent assay (elisa). within 21 days after inoculation, c6d was able ...19846094985
immunotoxic effects of diethylstilbestrol on host resistance: comparison with evaluate the usefulness of host resistance assays for measurement of immunotoxicologic effects of chemicals, the immunosuppressive effects of exposure to diethylstilbestrol (des) were compared with the effects of treatment with the known immunosuppressive drug cyclophosphamide (cps). a panel of six host resistance models was evaluated, including infection with the bacterium listeria monocytogenes, herpes simplex virus type 2 (hsv-2), and encephalomyocarditis virus (emc), the yeast cryptococcu ...19846323603
characterization of the 92,000-dalton glycoprotein induced by herpes simplex virus type 2.evidence is presented showing that the 92,000-dalton glycoprotein (g92k) induced by herpes simplex virus (hsv) type 2 has properties distinct from those assigned to any other hsv glycoprotein. first, the carbohydrate composition and extent of sulfation differ from those of glycoproteins d and e. second, two clonally unrelated monoclonal antibodies, ap1 and lp5, shown in this paper to specifically immunoprecipitate g92k, do not react with any of the known processed forms of glycoproteins b, c, d, ...19846323754
resistance of herpes simplex virus to 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine: physical mapping of drug synergism within the viral dna polymerase locus.a herpes simplex virus type 2 (hsv-2) mutant ts6 (strain hg52) induces a heat-labile viral dna polymerase at the nonpermissive temperature and is markedly resistant to 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]-guanine [2'-nor-2'-deoxyguanosine; 2'ndg]. this antiviral drug requires hsv thymidine kinase for phosphorylation to an active inhibitor (2'ndg-triphosphate), and thymidine kinase-deficient mutants of hsv exhibit varying degrees of resistance to 2'ndg, with the hsv type 1 (hsv-1) b2006 ...19846324203
herpes simplex virus infection causes the accumulation of a heat-shock protein.a monoclonal antibody, produced from mice immunized with a herpes simplex virus (hsv)-infected cell extract, reacts with a molecule which is present in uninfected cells and which accumulates in large amounts during hsv 2 infection. in uninfected cells this molecule is growth regulated, in that exponentially growing cells have intense nuclear immunofluorescence, whereas confluent quiescent cells have little. it has a mol. wt. of 57 000 (p57) in exponential cells, and one of 61 000 (p61) in quiesc ...19846325164
synthesis of some phosphonates with antiherpetic activity.several keto phosphonates, phosphonoacetates , and dialkyl phosphonates containing (aryloxy)aryl groups were synthesized and evaluated for antiherpteic activity. two of the most active compounds, 12 and 16, were evaluated topically in the mouse vaginal model against herpes simplex virus (hsv) type 2. compound 16 exhibited an increased survival rate, as well as increased survival time. evaluation of 16 in the guinea pig skin test against hsv-2 produced a reduction in virus titer, as well as in me ...19846325695
"recurrent herpes zoster": an unproved entity?physicians sometimes misdiagnose recurrent zosteriform herpes simplex virus (hsv) skin infections as "zoster" or "recurrent zoster." misdiagnosis can lead to inappropriate therapy with potentially harmful consequences, particularly in patients with ophthalmitis or immunosuppression, in which early institution of the correct antiviral therapy may be crucial. we report three patients who were originally misdiagnosed to have recurrent herpes zoster skin infections before we cultured hsv from their ...19846327783
secretory iga antibody in cervicovaginal secretions from women with genital infection due to herpes simplex virus.sequential samples of cervicovaginal secretions from women with untreated first and recurrent episodes of genital infection due to herpes simplex virus type 2 (hsv-2) were assayed for iga antibodies to hsv-2 by using fluorescent antibodies to human secretory piece (siga) and human iga. among women with first-episode genital herpes, siga antibody to hsv-2 was detected in 20 of 31 women from whom hsv was isolated from the cervix, compared with four of 13 women from whom it was not (p less than .05 ...19846327834
immunogenicity of herpes simplex virus glycoproteins gc and gb and their role in protective immunity.the relative antigenicity of the individual herpes simplex virus type 1 (kos) glycoproteins gc and gb was analyzed in balb/c mice by using kos mutants altered in their ability to present these antigens on cell surface membranes during infection. the mutants employed were as follows: syn ld70 , a non-temperature-sensitive mutant defective in the synthesis of cell surface membrane gc; tsf13 , a temperature-sensitive mutant defective in the processing of the precursor form of gb to the mature cell ...19846328010
physical mapping of the herpes simplex virus type 2 nuc- lesion affecting alkaline exonuclease activity by using herpes simplex virus type 1 deletion clones.the nuc- lesion affecting alkaline exonuclease activity in the herpes simplex virus type 2 (hsv-2) mutant ts1348 had previously been mapped to the ecori-d restriction enzyme fragment of hsv-1. eight clones with deletions representing most of hsv-1 ecori fragment d were selected with lambda gtwes hybrids. these clones were tested for their ability to rescue the alkaline exonuclease activity of hsv-2 nuc- ts1348 virus. the sequences colinear with the hsv-2 nuc- lesion were found to map between 0.1 ...19846328043
glycoprotein c of herpes simplex virus 1 acts as a receptor for the c3b complement component on infected cells.receptors for the fc portion of immunoglobulins or for the third component of complement (c3) are present on a variety of circulating and fixed tissue cells including granulocytes, monocytes, lymphocytes and glomerular epithelial cells. cells which lack fc receptors may express them after infection by herpes simplex virus (hsv)-1, hsv-2, cytomegalovirus or varicella zoster virus. we recently reported that infection by hsv-1 induces both fc and c3 receptors on human endothelial cells. glycoprotei ...19846328323
characterization of an aphidicolin-resistant mutant of herpes simplex virus type 2 which induces an altered viral dna polymerase.the replication of wild-type herpes simplex virus type 2 (hsv-2) was very sensitive to aphidicolin, a specific inhibitor of eukaryotic alpha-type dna polymerases; viral dna synthesis was strongly inhibited by 1 microgram/ml of aphidicolin, but the synthesis of early viral polypeptides was not affected. using aphidicolin as the selective agent, aphidicolin-resistant ( aphr ) viruses were isolated from hsv-2 strain 186. all of these plaque isolates induced altered viral dna polymerases which were ...19846328755
phosphorylation of four acyclic guanosine analogs by herpes simplex virus type 2 thymidine kinase.four acyclic guanosine analogs, the (r) and (s) enantiomers of 9-(3,4-dihydroxybutyl)guanine, 9-(4-hydroxybutyl)guanine, and acyclovir were compared in enzyme kinetic experiments, using purified herpes simplex virus type 2 thymidine kinase. all four analogs showed competitive patterns of inhibition in the phosphorylation of thymidine by the viral thymidine kinase, but different affinities and relative rates of phosphorylation were observed.19846329085
direction of synthesis of the message for a 35 000 polypeptide of herpes simplex virus type 2.a restriction endonuclease map of the coding sequences for the transformation-related 35 000 polypeptide of herpes simplex virus type 2 is reported, using 11 enzymes that cut dna infrequently. hybrid-arrested translation with small dna fragments was used to locate the 5' terminus of these coding sequences. a novel procedure involving translation of the 35 000 mrna selected with cloned viral dna that had been digested with exonuclease was employed to confirm that this message is transcribed from ...19846329490
herpes virus antigens and antibodies in kidney biopsies and sera of iga glomerulonephritic patients.antibody titers against 4 viruses of the herpes group have been studied in the sera of 54 patients with iga glomerulonephritis (gn). the incidence of antibodies to herpes simplex virus type-1 (hsv-1), hsv-2, cytomegalovirus (cmv), epstein-barr virus nuclear antigen (ebna), and that of igg antibodies to ebv virus capsid antigen (vca) were similar in patients and in controls. however, elevated antibody titers (greater than or equal to 64) to hsv and cmv occurred more frequently in patients with ig ...19846329570
expression of a herpes simplex virus glycoprotein in two clonal lines of hamster cells transformed by herpes simplex virus type 2.expression of herpes simplex virus type 2 (hsv-2) glycoproteins on the cell surface of two clones of a hamster cell line transformed by hsv-2 was examined by immunofluorescence tests with hamster antisera prepared against three size-class glycoprotein fractions obtained from hamster embryo fibroblast cells infected with hsv-2. enhanced expression of one of the three size-class glycoprotein fractions (molecular weight, 82,000-94,000) was found in one clone (155-4-213) after replating of the cells ...19846329882
structural organization of human herpesvirus dna molecules.the herpesviruses are among the largest and most complex of all dna viruses, and their genomes display an astonishing diversity in size, structure, and organization. in 1974, the features of large inverted repeats and structural isomerization were first discovered, and these proved to be characteristic properties of many herpesvirus genomes. since then, research using the powerful techniques of modern molecular biology has revealed a great deal of comparative structural information about the arr ...19846330219
genital herpes simplex infections: effect of asymptomatic shedding and latency on management of infections in pregnant women and neonates.asymptomatic shedding of herpes simplex virus (hsv) at delivery occurred in 2.4% of women with a past history of recurrent genital infections; using current methods, this could not be predicted in advance. in addition, only 22% of the mothers of infected infants had an elicitable history of recurrent genital infections. passively derived neutralizing antibody titers of 1:106 to hsv-1 and 1:67 to hsv-2 were found in 22 exposed infants who remained asymptomatic as compared with 1:8 and 1:8, respec ...19846330222
herpes simplex virus type 2 establishes latency in the mouse footpad and in the sensory ganglia.three mouse strains as well as wt and 7 ts mutants of herpes simplex virus (hsv) type 2 (strain hg52) have been used to investigate latency. the mice were inoculated in the right rear footpad. virus reactivation following explanation and culture of the dorsal root ganglia and the footpad was scored. the results show that: hsv-2 can be maintained in the mouse footpad in a state indistinguishable from latency; virus gene functions necessary for latency can be identified by the use of ts mutants; a ...19846330225
hsv, cmv, and hpv in human neoplasia.we are studying the role of sexually transmitted viruses in the development of human tumors. the persistence of herpes simplex virus, cytomegalovirus, and human papillomavirus nucleic acid sequences has been examined using cloned viral dna sequences as probes. the relationship of the viruses to various stages in the progression of neoplasia is examined, with particular reference to the role of viral and/or cellular genes in the initiation, promotion, and maintenance of the neoplastic phenotype. ...19846330227
determination of herpes simplex virus type-specific antibodies by solid-phase ria on helix pomatia lectin-purified antigens.herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with helix pomatia lectin/sepharose 4b-purified antigens from hsv-1- and hsv-2-infected cells. a low but constant heterotypic reactivity was found with hsv-1 antigen, whereas hsv-2 antigen was type specific, as evidenced by (1) its reactivity with sera from hsv-2- but not with those from hsv-1-immunized animals, (2) its reactivity with monoclonal antibod ...19846330240
a rapid method for restriction endonuclease analysis of dna from herpes simplex virus type 2. 19846330274
evaluation of the effectiveness of 2h-1,3-oxazine-2,6(3h)-dione (oxauracil) in the treatment of herpes simplex virus (hsv)-induced acute encephalitis in mice.treatment of suckling mice with the pyrimidine analogue 2h-1,3-oxazine-2,6(3h)-dione (oxauracil) proved successful in reducing mortality associated with hsv-2-induced encephalitis. oxauracil did not decrease mortality associated with hsv-1 infection; however, there was an increased survival time and a decrease in the amount of infectious virus recovered from the brains of hsv-1-infected animals.19846330291
homology between two ebv early genes and hsv ribonucleotide reductase and 38k of amino acid sequences predicted from the complete ebv dna sequence against the known hsv gene sequences has revealed significant homology between two ebv reading frames and the hsv1 and hsv2 140k and 38k proteins which are associated with ribonucleotide reductase activity. the two genes are arranged tandemly as in hsv though it appears that, unlike hsv, the two mrnas are not 3' co-terminal. we have mapped two promoters predicted from the dna sequence for these genes and shown ...19846330697
nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide.the nucleotide sequence of a 2549-bp dna fragment containing the entire coding region of the marmoset herpesvirus (marhv) thymidine kinase gene (tk) and the flanking sequences was determined by the dideoxynucleotide chain termination method. the marhv thymidine kinase polypeptide predicted from the nucleotide sequence contained 376 amino acids and had a molecular weight of 41,281. the sequencing data also reveal that the coding portion of another marhv gene probably begins only 292 nucleotides d ...19846330976
enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with alpha-interferon against herpes simplex virus type 2 in mice.the acyclic nucleoside dhpg [9-(1,3-dihydroxy-2-propoxymethyl)guanine] and recombinant human alpha-interferon of clones a/d potentiate each other's antiviral activity against a systemic infection with herpes simplex virus type 2. the effective dose at which 50% of the mice survived was lowered approximately 10-fold for dhpg when it was given in combination with a marginally effective dose of alpha-interferon and greater than 10-fold for alpha-interferon when it was given in combination with a no ...19846335382
early interactions between human cytomegalovirus and cells.adsorption of cytomegalovirus (cmv) to human fibroblasts was not inhibited by preincubation with other herpes viruses (hsv-1, hsv-2, vzv). transport of virus to the nucleus was studied using virus labelled with 3h-thymidine. radioactivity was found in the nucleus 20 minutes after virus had been added to the cells. in order to assess the expression of the virus genome, synthesis of early (ea) and late (la) antigens was studied. assayed on permissive human lung fibroblasts, a plaque purified virus ...19846320772
dna polymerase in nuclei isolated from herpes simplex virus type-2-infected cells. characterization of the reaction product and inhibition by substrate analogs.nuclei isolated from herpes simplex virus (hsv) type 2-infected kb cells were examined for their capacity to serve as an in situ source of herpes dna polymerase. in contrast to purified enzymes with added template, approx. 80% of the dna synthesized in isolated nuclei was viral. the average size of dna fragments labeled in vitro was 3.2 x 10(6) da. based on an increase in dna density when nuclei were incubated in the presence of brdutp rather than dttp, 16% of the nucleotides were added during t ...19846320890
prospective study on the relationship between cervical neoplasia and herpes simplex type-2 virus. ii. herpes simplex type-2 antibody presence in sera taken at enrollment.sera obtained at enrollment in the study from patients suffering from moderate to sever dysplasia (cervical intraepithelial neoplasia grade ii), carcinoma in situ (cervical intraepithelial neoplasia grade iii) and invasive carcinoma, or developing any of these conditions in the course of the prospective study, and from control subjects, were examined for herpes simplex type-2 (hsv-2) antibody presence. the controls were matched with the patients by age, age at first intercourse, number of sexual ...19846319297
recovery of herpes simplex virus genetic information from human trigeminal ganglion cells following superinfection with herpes simplex virus type 2 temperature-sensitive mutants.explant cultures of human trigeminal ganglia were derived from 36 individuals. those cultures which failed to release herpes simplex virus (hsv) spontaneously were superinfected at various times after establishment in vitro with a range of hsv-2 temperature-sensitive (ts) mutants. eight cultures from six individuals contained hsv-specific genetic information which could be detected or rescued following superinfection. restriction enzyme analysis of ts+ virus recovered from the ganglia of two ind ...19846319568
class-specific antibody responses to early and late antigens of varicella and herpes simplex viruses.antibody responses to early antigens of varicella-zoster virus (vzv), simian varicella virus, and herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were studied in primary, secondary, and latent infections. igg antibody responses to the early antigens occurred in primary and secondary vzv and hsv infections, and antibodies to early antigens were also demonstrable in healthy individuals with latent vzv and hsv infections, indicating that the presence of antibodies to early antigens cannot be t ...19846319583
incidence of herpes simplex virus types 1 and 2 in penile lesions of college men.herpes simplex virus (hsv) was isolated from penile lesions of 15 college men. using (e)-5-(2-bromovinyl)-2'-deoxyuridine sensitivity, monoclonal antibody immunofluorescence, and restriction endonuclease ecori digestion of viral dna, 4 of 15 (26%) isolates were found to be hsv-1, and 11 of 15 (74%) isolates were found to be hsv-2. it is likely that some of the genital hsv-1 infections are related to oral genital contact, but this fact could not be established for all cases, since the females in ...19846319589
amino-terminal sequence of glycoprotein d of herpes simplex virus types 1 and 2.glycoprotein d (gd) of herpes simplex virus is a structural component of the virion envelope which stimulates production of high titers of herpes simplex virus type-common neutralizing antibody. we carried out automated n-terminal amino acid sequencing studies on radiolabeled preparations of gd-1 (gd of herpes simplex virus type 1) and gd-2 (gd of herpes simplex virus type 2). although some differences were noted, particularly in the methionine and alanine profiles for gd-1 and gd-2, the amino a ...19846317892
treatment of primary acute genital herpes in guinea pigs by intraperitoneal administration of fluoropyrimidines.fiac [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine], fiau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil], and fmau [1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil] were evaluated for their efficacies in the treatment of genital infections with herpes simplex virus type 2 in guinea pigs. intraperitoneal administration of these drugs in daily doses of 100 mg/kg of body weight initiated 24 h after virus inoculation and repeated 2 successive days therea ...19846239591
clonal analysis of t-cell responses to herpes simplex virus: isolation, characterization and antiviral properties of an antigen-specific helper t-cell clone.a herpes simplex virus (hsv)-specific long-term t-cell clone has been established from the draining lymph node cells of balb/c mice; the cells required repeated in vitro restimulation with uv-irradiated virus. the established t-cell clone expresses the thy-1 and lyt-1+2,3- surface antigens. for optimal proliferation of the cloned cells, both the presence of specific antigen and an exogenous source of t-cell growth factor are required. the proliferative response of the cloned t cells was found to ...19846209206
immunofluorescent evidence of prior herpes simplex virus type-2 infection in prostate carcinoma.the finding of herpes simplex virus type-2 (hsv-2) particles in prostatic carcinoma (pca) tissue has led to speculation that the virus might cause this disease. we studied 27 pca and 33 benign prostatic hyperplasia (bph) specimens for the presence of hsv-2 antigens by indirect immunofluorescent staining to hsv-2 using commercially prepared rabbit anti-hsv-2 and fluorescein-tagged goat and anti-rabbit antibody. the slides were randomly number coded by an impartial referee then read independently ...19846209848
ultrastructural changes during herpes simplex virus type 2 latency and reactivation in in vitro latency system using herpes simplex virus type 2(hsv2)-infected human embryonic lung cells treated with cytosine arabinoside (ara-c) and incubated at 39.5 degrees after drug removal was used to examine the ultrastructure of the infected cells during (i) ara-c treatment, (ii) the latent period after ara-c removal and temperature elevation, and (iii) reactivation after superinfection with human cytomegalovirus. in the presence of ara-c 'empty', nonenveloped, herpesvirus-like particles ...19846698759
systemic herpes simplex virus type 2 infection. proctitis, urinary retention, arthralgias, and meningitis in the absence of primary mucocutaneous lesions.a 24-year-old heterosexual man had severe proctalgia associated with nonspecific proctitis. within a few days, perineal and lower extremity paresthesias, intermittent urinary retention, inguinal lymphadenopathy, lower extremity arthralgias, and aseptic meningitis developed. serologic studies demonstrated a fourfold rise in convalescent antibody titer to herpes simplex virus (hsv) consistent with an initial type 2 infection. six months later, hsv type 2 was isolated from perianal vesicles, which ...19846712380
thymine arabinoside (ara-t) topical and iontophoretic application for herpes simplex virus type 1 and type 2 skin infections in hairless mice.the antiviral agent thymine arabinoside (9-beta-d-arabinofuranosyl thymine, ara-t) was applied topically and by anodal (+) iontophoresis in a nacl solution to herpes simplex virus type 1 (hsv-1), and herpes simplex virus type 2 (hsv-2) skin infections in hairless mice. the chemotherapeutic efficacy of ara-t was evaluated employing the parameters of mean lesion score, number of mice with signs of neurological involvement (paralysis), mortality and mean survival time. anodal (+) iontophoresis of 0 ...19846717166
heterogeneity in subregions of the terminal repeats of herpes simplex virus type 2 dna.we constructed recombinant plasmids from the 0.5 molar terminal fragments of herpes simplex virus type 2 (hsv2) dna. physical mapping of three different l-terminal clones demonstrated one, two or three copies of the terminally redundant a repeat. the nucleotide sequence at the end of the terminal a repeats was shown to be identical in these three cloned hsv2 genomic l-termini and closely resembled the internal a' repeat sequence reported previously by davison and wilkie. virtually all clones tha ...19846321396
herpes simplex virus type-selective enzyme-linked immunosorbent assay with helix pomatia lectin-purified antigens.helix pomatia lectin-purified antigens with specific reactivity to herpes simplex virus type 1 (hsv-1) and hsv-2 antibodies in human sera were used in an enzyme-linked immunosorbent assay. the type specificity of the antigens was assessed by double immunodiffusion precipitation in gel against rabbit hsv-1 and hsv-2 hyperimmune sera, and by enzyme-linked immunosorbent assay with human reference sera containing antibodies to either type of hsv. fifty-two sera from patients with documented infectio ...19846321548
the junctions between the repetitive and the short unique sequences of the herpes simplex virus genome are determined by the polypeptide-coding regions of two spliced immediate-early mrnas.immediate-early (ie) mrnas-4 and -5 of herpes simplex virus type 2 (hsv-2) are transcribed from the irs/trs genome regions towards the us region. each of these spliced mrnas has an untranslated leader sequence of 249 bases and a single intron of approximately 540 bases which are contained entirely within trs/irs sequences. the dna sequence of the intron largely comprises tandem reiterations of three distinct short sequences. upstream of the common 5' mrna termini the dna sequence contains region ...19846321634
differential substrate specificity of dna polymerase beta and of a dna polymerase induced by herpes simplex virus type 2 towards thymidine triphosphate analogues.several triphosphates of 5-substituted deoxyuridine (du), such as 5-ethyl-, 5-n-propyl-, 5-n-hexyl- and 5-isopropyldeoxyuridine triphosphates and 5-trifluorothymidine triphosphate are substrates for hela cell dna polymerase beta (2'-deoxynucleoside-5'-triphosphate:dna-deoxynucleotidyltransferase, ec and for a dna polymerase isolated from hela cells infected with herpes simplex virus type 2 (hsv-2) strain 75. at the concentration tested (50 microm), all these analogues were incorporated ...19846321635
intra-axonal virus in demyelinative lesions of experimental herpes simplex type 2 infection.three-week-old mice which had been infected intracerebrally with herpes simplex virus type 2 (hsv-2) were examined electron-microscopically for the presence of intra-axonal virus in or near optic nerve and spinal cord demyelinative lesions. acute lesions and their margins frequently contained a very small proportion of abnormal axons, and in a few of these mature virus particles, nucleocapsids, or other incomplete forms were found. a similar range of particle morphology was present in the cytopl ...19846321668
synthesis of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine and related analogues: potent and unusually selective antiviral activity of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine against herpes simplex virus type 1.syntheses of (e)-5-(3,3,3-trifluoro-1-propenyl)-2'-deoxyuridine (tfpe-durd) (1), 5-(3,3,3-trifluoro-1-propyl)-2'-deoxyuridine (11), 5-(3,3,3-trifluoro-1-methoxy-1-propyl)-2'-deoxyuridine (8), and 5-(3,3,3-trifluoro-1-hydroxy-1-propyl)-2'-deoxyuridine (10) from 5-chloromercuri-2'-deoxyuridine are described. the antiviral activity of tfpe-durd was determined in cell culture against herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), and vaccinia virus and compared concurrentl ...19846321735
organization of the left-hand end of the herpes simplex virus type 2 bglii n fragment.we have determined the complete nucleotide sequence surrounding the region coding for a 38,000-dalton protein of herpes simplex virus type 2 strain 333 that is encoded between map coordinates 0.58 to 0.595 on the viral genome. the sequence data have revealed an open translational reading frame of 1,011 nucleotides encoding a protein of 337 amino acids. upstream of those sequences, transcriptional regulatory signals could be identified that overlap another open reading frame of 489 nucleotides, w ...19846321759
mapping of the structural gene for the herpes simplex virus type 2 counterpart of herpes simplex virus type 1 glycoprotein c and identification of a type 2 mutant which does not express this glycoprotein.the gene encoding glycoprotein f (gf) of herpes simplex virus type 2 (hsv-2) was mapped to the region of the viral genome from 0.62 to 0.64 map units. this region is colinear with, and partially homologous to, the region of the hsv-1 genome previously shown to encode gc. mapping of the gf gene was done by insertion of hsv-2 dna fragments into the thymidine kinase gene of an hsv-1 virus and screening of the resultant recombinant viruses for the expression of gf. in this way, dna sequences necessa ...19846321760
mapping of a herpes simplex virus type 2-encoded function that affects the susceptibility of herpes simplex virus-infected target cells to lysis by herpes simplex virus-specific cytotoxic t lymphocytes.a function(s) involved in the altered susceptibility of herpes simplex virus type 2 (hsv-2)-infected cells to specific lysis by cytotoxic t lymphocytes was mapped in the s component of hsv-2 dna by using hsv-1 x hsv-2 intertypic recombinants (rh1g44, rs1g25, r50bg10, a7d, and c4d) and hsv-1 mp. target cells infected with r50bg10, a7d, and c4d exhibited reduced levels of cytolysis, as did hsv-2-infected cells, whereas rh1g44 and rs1g25 recombinant-infected and hsv-1 mp-infected cells showed level ...19846321762
limited proteolysis of herpes simplex virus glycoproteins that occurs during their extraction from vero cells.herpes simplex virus glycoproteins extracted from infected vero cells can be smaller in apparent size than the same viral products extracted from infected hep-2 cells. here we show that the differences in size result primarily from limited proteolysis, during or after their extraction, of the viral glycoproteins made in vero cells. in the absence of appropriate protease inhibitors, both mature and immature forms of four different glycoproteins specified by herpes simplex virus type 2 were signif ...19846321794
replication origins and a sequence involved in coordinate induction of the immediate-early gene family are conserved in an intergenic region of herpes simplex virus.we have determined the structure of the 5' portion of herpes simplex virus type 2 (hsf-2) immediate-early (ie) mrna-3 and have obtained the dna sequence specifying the n terminus of its encoded polypeptide, vmw182, its untranslated leader and the intergenic region between iemrnas-3 & 4/5. comparison of the hsv-2 intergenic sequences with the hsv-1 equivalent region identifies several conserved regions: (1) an at-rich element with core consensus taatgarat which is likely to be the 'activator' seq ...19846322134
polypeptide specificity of the early antibody response following primary and recurrent genital herpes simplex virus type 2 infections.relative igg titres to specific viral proteins in acute and convalescent sera from patients with primary and recurrent genital herpes simplex virus type 2 (hsv-2) infections were determined using an immunoblot assay. patients with recurrent genital infections generally had high titres of antibody reactive with two of the three major viral glycoproteins (gd and gg) as well as with several viral capsid proteins in both acute and convalescent sera. in contrast, patients experiencing primary genital ...19846387042
epidemiological aspects of cervical cancer in tropical africa.cervical cancer is one of the most common malignant tumours in tropical africa accounting, for example, for nearly one-quarter of female cancer cases overall in uganda. the disease is likely to be under-reported and available incidence rates are probably gross underestimates. spread is through sexual contacts. both female and male promiscuity and a low standard of sexual hygiene lead to a high incidence within a given community. a number of investigations show that the risk of cervical cancer va ...19846399280
carcinoma of the cervix: can a viral etiology be confirmed?the etiological role of viruses in various animal cancers is no longer in doubt; a similar role in human cancers is, however, yet to be confirmed. indeed, until recently, virus infections were considered to be responsible for only a small proportion of the clinical states encountered in humans and the view that viruses may be etiological agents for human malignant tumours was not seriously entertained. with the introduction of more advanced techniques in immunovirology, the role of viruses in cl ...19846399281
the presence of rna complementary to hsv-2 (herpes simplex virus) dna in cervical intraepithelial neoplasia after laser hybridization of radiolabelled dna probes to tissue sections detected rna complementary to hsv-2 (herpes simplex virus) dna in 65% (22 of 34) biopsies from cervical intraepithelial neoplasia (cin) and in none of internally paired benign epithelia from individuals before treatment by laser vaporization cone. laser therapy did not produce an increase in clinical or subclinical hsv infections of the cervix. of 18 patients with rna complementary to hsv-2 dna in cin biopsies before treatment, ...19846322834
calcium ionophore a-23187 and 12-o-tetradecanoyl-phorbol-13-acetate stimulation of prostaglandin synthesis in herpes simplex virus type 2-transformed rat cells.the purpose of this investigation was to determine whether cells transformed by herpes simplex virus type 2 (hsv-2) can be stimulated to synthesize prostaglandins (pg). stimulation was determined by measuring the release of pg into overlay fluids from cell monolayers prelabeled with [3h]arachidonic acid. results showed that ca2+ ionophore a-23187 markedly stimulated arachidonic acid release starting 30 min after treatment of hsv-2-transformed and nontransformed rat embryo fibroblast cells. howev ...19846322982
enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with beta-interferon against herpes simplex virus type 2 in mice.the acyclic nucleoside 9-(1,3-dihydroxy-2- propoxymethyl )guanine (dhpg) and natural mouse interferon beta ( muifn -beta) were evaluated for their efficacy alone and in combination against herpes simplex virus type 2 systemic infections in mice. intraperitoneally infected animals were treated once a day with the drugs at various concentrations for 5 days starting 24 h after inoculation. dhpg was injected subcutaneously at doses of 0.7 to 6 mg/kg. muifn -beta was given intraperitoneally at doses ...19846610387
detection of herpes simplex virus intertypic recombinant genomes in infected cell dna.intertypic recombination between herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was detected using dna from mixedly infected cells. because hsv-1 and hsv-2 share a 50% base sequence homology along the genome but have markedly different dna restriction enzyme cleavage patterns, recombination events can be detected and quantified by analysis of restriction endonuclease digests for the presence of novel dna fragments. we have used this technique to quantify the degree of interference by hsv-2 ...19846323508
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