Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| induction of interferon-λ contributes to tlr3 and rig-i activation-mediated inhibition of herpes simplex virus type 2 replication in human cervical epithelial cells. | is it possible to immunologically activate human cervical epithelial cells to produce antiviral factors that inhibit herpes simplex virus type 2 (hsv-2) replication? | 2015 | 26502803 |
| detection of virus in csf from the cases with meningoencephalitis by next-generation sequencing. | we screened for viral dna in cerebrospinal fluid samples using next-generation sequencing (ngs) technology to diagnose cns viral infections. we collected csf samples from four cases with clinically suspected viral meningoencephalitis. dna extracted from the samples was analyzed with ngs, and the results were further validated using pcr. herpes simplex virus 1 (hsv-1) was detected in the csf of two patients, hsv-2 and human herpes virus type 3 (hhv-3, vzv) in the csf of two other patients separat ... | 2016 | 26506841 |
| global and regional estimates of prevalent and incident herpes simplex virus type 1 infections in 2012. | herpes simplex virus type 1 (hsv-1) commonly causes orolabial ulcers, while hsv-2 commonly causes genital ulcers. however, hsv-1 is an increasing cause of genital infection. previously, the world health organization estimated the global burden of hsv-2 for 2003 and for 2012. the global burden of hsv-1 has not been estimated. | 2015 | 26510007 |
| herpes simplex virus and varicella zoster virus: recent advances in therapy. | the mainstay of antiviral therapy for the alpha-herpesviruses [herpes simplex virus (hsv)-1, hsv-2, and varicella zoster virus (vzv)] over the past 40 years has been the nucleoside analogues such as aciclovir. although conventional antiviral therapy has reduced mortality in severe disease, novel agents are needed to address the emergence of resistance and toxicity associated with current second-line therapy. treatment and prophylaxis of vzv and hsv reactivations remains a challenge. | 2015 | 26524331 |
| multiplex pcr testing for nine different sexually transmitted infections. | current sexually transmitted infection (sti) testing is not optimal due to delays in reporting or missed diagnoses due to a lack of comprehensive testing. the filmarray® (biofire diagnostics, llc, salt lake city, utah) is a user-friendly, fully automated, multiplex pcr system that is being developed for rapid point-of-care use. a research-use-only sti panel including multiple pcr primer sets for each organism was designed to detect chlamydia trachomatis, neisseria gonorrhoeae, treponema pallidum ... | 2016 | 26538551 |
| herpes simplex virus type 2 encephalitis as a cause of ischemic stroke: case report and systematic review of the literature. | our objective is to describe a patient who developed an ischemic stroke as a complication of herpes simplex virus type 2 (hsv-2) encephalitis and to review the literature. | 2016 | 26542825 |
| herpes simplex virus downregulation of secretory leukocyte protease inhibitor enhances human papillomavirus type 16 infection. | herpes simplex virus (hsv) was originally implicated in the aetiology of cervical cancer, and although high-risk human papillomavirus (hpv) is now the accepted causative agent, the epidemiological link between hsv and hpv-associated cancers persists. the annexin a2 heterotetramer (a2t) has been shown to mediate infectious hpv type 16 (hpv16) uptake by human keratinocytes, and secretory leukocyte protease inhibitor (slpi), an endogenous a2t ligand, inhibits hpv16 uptake and infection. interesting ... | 2016 | 26555393 |
| simultaneous quantification of the 8 human herpesviruses in allogeneic hematopoietic stem cell transplantation. | human herpesviruses may cause severe complications after allogeneic hematopoietic stem cell transplantation (hsct). however, the impact of some of these infections on transplant outcomes is still unclear. a prospective survey on the incidence and clinical features of herpesviruses infections after hsct has not yet been conducted in brazilian patients, and the impact of these infections on hsct outcome remains unclear. | 2016 | 26555946 |
| gene expression profiles from disease discordant twins suggest shared antiviral pathways and viral exposures among multiple systemic autoimmune diseases. | viral agents are of interest as possible autoimmune triggers due to prior reported associations and widely studied molecular mechanisms of antiviral immune responses in autoimmunity. here we examined new viral candidates for the initiation and/or promotion of systemic autoimmune diseases (said), as well as possible related signaling pathways shared in the pathogenesis of those disorders. rna isolated from peripheral blood samples from 33 twins discordant for said and 33 matched, unrelated health ... | 2015 | 26556803 |
| the "knife-cut sign" revisited: a distinctive presentation of linear erosive herpes simplex virus infection in immunocompromised patients. | the "knife-cut sign" is a distinctive presentation of linear erosive herpes simplex virus infection in immunocompromised patients. | 2015 | 26557219 |
| seroprevalence of herpes simplex virus infection in hiv coinfected individuals in eastern india with risk factor analysis. | herpes simplex virus type 2 (hsv-2) is the cause of most genital herpes while hsv-1 is responsible for orolabial and facial lesions. in immunocompromised individuals, like hiv patients, impaired immunity leads to more frequent symptomatic and asymptomatic hsv infection. fifty-two blood samples from hiv patients with clinically diagnosed hsv infection were taken as cases, while 45 blood samples each from hiv-infected (hiv control) and noninfected patients without any herpetic lesion (non-hiv cont ... | 2015 | 26557849 |
| herpes simplex virus 1 (hsv-1) and hsv-2 mediate species-specific modulations of programmed necrosis through the viral ribonucleotide reductase large subunit r1. | receptor-interacting protein kinase 3 (rip3) and its substrate mixed-lineage kinase domain-like protein (mlkl) are core regulators of programmed necrosis. the elimination of pathogen-infected cells by programmed necrosis acts as an important host defense mechanism. here, we report that human herpes simplex virus 1 (hsv-1) and hsv-2 had opposite impacts on programmed necrosis in human cells versus their impacts in mouse cells. similar to hsv-1, hsv-2 infection triggered programmed necrosis in mou ... | 2015 | 26559832 |
| a herpes simplex virus 2 (hsv-2) gd mutant impaired for neural tropism is superior to an hsv-2 gd subunit vaccine to protect animals from challenge with hsv-2. | a recent phase 3 trial with soluble herpes simplex virus 2 (hsv-2) glycoprotein d (gd2t) in adjuvant failed to show protection against genital herpes. we postulated that live attenuated hsv-2 would provide more hsv antigens for induction of virus-specific antibodies and cellular immunity than would gd2t. we previously reported an hsv-2 mutant, hsv2-gd27, in which the nectin-1 binding domain of gd2 is altered so that the virus is impaired for infecting neural cells, but not epithelial cells, in v ... | 2015 | 26559846 |
| current concepts for genital herpes simplex virus infection: diagnostics and pathogenesis of genital tract shedding. | herpes simplex virus 2 (hsv-2) is a dna virus that is efficiently transmitted through intimate genital tract contact and causes persistent infection that cannot be eliminated. hsv-2 may cause frequent, symptomatic self-limited genital ulcers, but in most persons infection is subclinical. however, recent studies have demonstrated that the virus is frequently shed from genital surfaces even in the absence of signs or symptoms of clinical disease and that the virus can be transmitted during these p ... | 2016 | 26561565 |
| high rate of β-globin dna detection validates self-sampling in herpes simplex virus shedding studies. | self-sampling is a convenient, feasible, and acceptable way of collecting genital specimens, but the veracity of reported self-collection is difficult to verify. we investigated whether a host gene, β-globin, can be used to confirm adequacy of self-collected mucosal and skin genital specimens in studies of genital herpes simplex virus type 2 (hsv-2) infection. | 2015 | 26562701 |
| predictors of outcome in hsv encephalitis. | this study aims to explore the clinical features, radiological findings, management and the factors influencing prognosis in pcr-confirmed herpes simplex virus encephalitis (hse). this is a retrospective review of consecutive patients diagnosed with hse at mayo clinic, rochester, mn, between january 1995 and december 2013. only hse cases confirmed by pcr were included. univariate and multivariate analysis was used to identify factors associated with good (modified rankin scale of 0-2) or poor ou ... | 2016 | 26568560 |
| study of the frequency of herpesvirus infections among patients suspected aseptic meningitis in the west of iran. | aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). | 2015 | 26568804 |
| a novel hsv-2 subunit vaccine induces gla-dependent cd4 and cd8 t cell responses and protective immunity in mice and guinea pigs. | there is currently no licensed prophylactic or therapeutic vaccine for hsv-2 infection. | 2016 | 26571309 |
| hexagonal-shaped chondroitin sulfate self-assemblies have exalted anti-hsv-2 activity. | the initial step in mucosal infection by the herpes simplex virus type 2 (hsv-2) requires its binding to certain glycosaminoglycans naturally present on host cell membranes. we took advantage of this interaction to design biomimetic supramolecular hexagonal-shaped nanoassemblies composed of chondroitin sulfate having exalted anti-hsv-2 activity in comparison with native chondroitin sulfate. nanoassemblies were formed by mixing hydrophobically-modified chondroitin sulfate with α-cyclodextrin in w ... | 2016 | 26572336 |
| effect of condom use on per-act hsv-2 transmission risk in hiv-1, hsv-2-discordant couples. | the efficacy of condoms for protection against transmission of herpes simplex virus type 2 (hsv-2) has been examined in a variety of populations with different effect measures. often the efficacy has been assessed as change in hazard of transmission with consistent vs inconsistent use, independent of the number of acts. condom efficacy has not previously measured on a per-act basis. | 2016 | 26578538 |
| [novel multiplex real-time pcr assay for detection and differentiation of herpes simplex virus type 1 and 2 dna]. | herpes simplex viruses type 1 and 2 are the cause of world spread multiple infections with different course and severity. the aim of this work was to design and to optimize multiplex real-time pcr assay for simultaneous detection of hsv-1 and hsv-2. the second aim of the project was to check if the designed method is laboratory useful analyzing different clinical specimens. | 2015 | 26591664 |
| multipurpose prevention approaches with antiretroviral-based formulations. | we compared the preclinical safety and efficacy of tenofovir (tfv) 1% gel with that of mzc gel [containing 50 μm miv-150, 14 mm zn(o2cch3)2(h2o)2, and 3% carrageenan] through a series of in vitro, ex vivo, and in vivo assays. the two gels showed good antiviral therapeutic indexes (50% cytotoxic concentration/50% effective concentration ratios; range, >25 to 800). mzc showed greater anti-simian-human immunodeficiency virus reverse transcriptase (shiv-rt) activity than tfv 1% gel in rhesus macaque ... | 2016 | 26596943 |
| do viral infections have a role in benign paroxysmal positional vertigo? | to investigate the role of viral infection in benign paroxysmal positional vertigo (bppv). | 2015 | 26601554 |
| δpk oncolytic activity includes modulation of the tumour cell milieu. | oncolytic virotherapy is a unique cancer therapeutic that encompasses tumour cell lysis through both virus replication and programmed cell death (pcd) pathways. nonetheless, clinical efficacy is relatively modest, likely related to the immunosuppressive tumour milieu. our studies use the herpes simplex virus type 2 (hsv-2)-based oncolytic virus δpk that has documented anti-tumour activity associated with virus replication, pcd and cancer stem cell lysis. they are designed to examine whether δpk- ... | 2016 | 26602205 |
| protection from genital herpes disease, seroconversion and latent infection in a non-lethal murine genital infection model by immunization with an hsv-2 replication-defective mutant virus. | viral vaccines have traditionally protected against disease, but for viruses that establish latent infection, it is desirable for the vaccine to reduce infection to reduce latent infection and reactivation. while seroconversion has been used in clinical trials of herpes simplex virus (hsv) vaccines to measure protection from infection, this has not been modeled in animal infection systems. to measure the ability of a genital herpes vaccine candidate to protect against various aspects of infectio ... | 2016 | 26609935 |
| herpes simplex virus seroprevalence and seroconversion among active duty us air force members with hiv infection. | herpes simplex virus (hsv) infection is associated with an increased risk of both hiv transmission and acquisition. we evaluated longitudinal hsv serology and sexually transmitted infections (stis) among active duty us air force (usaf) members with hiv infection. | 2016 | 26615389 |
| toxicity and antiviral activity of the extracts of submerged mycelium of nematophagous duddingtonia flagrans fungus in vero cell culture. | we studied toxicity and antiviral activity of aqueous and ethanol extracts of bioactive substances from the biomass of nematophagous fungus duddingtonia flagrans prepared by submerged culturing of the mycelium. it is found that both extracts were characterized by low toxicity for cultured vero cells and inhibited reproduction of dna-viruses in this cell line. ethanol extract of the fungus exhibited higher in vitro antiviral activity against herpes simplex virus type 2, ectromelia virus, and vacc ... | 2015 | 26621278 |
| low prevalences of hiv infection and hsv genital shedding in the general adult female population in senegal. | herpes simplex virus (hsv) is the main co-factor for heterosexual transmission of the human immunodeficiency virus (hiv) in sub-saharan africa, and could be involved in the dynamics of the hiv epidemic in senegal. | 2015 | 26623637 |
| antiviral drug-resistance typing reveals compartmentalization and dynamics of acyclovir-resistant herpes simplex virus type-2 (hsv-2) in a case of neonatal herpes. | a neonate suffering from herpes simplex virus type 2 disease with central nervous system involvement developed an early recurrence under acyclovir therapy. isolates from the cerebrospinal fluid and skin lesions were acyclovir resistant, while viruses from blood and trachea were not. acyclovir combined with foscavir followed by long-term suppressive acyclovir therapy supported normal neurological development. | 2014 | 26625375 |
| herpes simplex virus infections of the central nervous system. | this article summarizes knowledge of herpes simplex virus (hsv) infections of the central nervous system (cns). disease pathogenesis, detection of dna polymerase chain reaction (pcr) for diagnosis and prognosis, and approaches to therapy warrant consideration. | 2015 | 26633784 |
| antiviral action of diphenyl diselenide on herpes simplex virus 2 infection in female balb/c mice. | diphenyl diselenide, (phse)2 , is an organoselenium compound with pharmacological actions mostly related to antioxidant and anti-inflammatory properties. the study investigated its antiviral and virucidal actions against herpes simplex virus 2 (hsv-2) infection in vitro and in a vaginal infection model in mice. the plaque reduction assay indicated that (phse)2 showed virucidal and antiviral actions reducing infectivity in 70.8% and 47%, respectively. the antiviral action of (phse)2 against hsv-2 ... | 2016 | 26639776 |
| the cholestanol-conjugated sulfated oligosaccharide pg545 disrupts the lipid envelope of herpes simplex virus particles. | herpes simplex virus (hsv) and many other viruses, including hiv, initiate infection of host cells by binding to glycosaminoglycan (gag) chains of cell surface proteoglycans. although gag mimetics, such as sulfated oligo- and polysaccharides, exhibit potent antiviral activities in cultured cells, the prophylactic application of these inhibitors as vaginal microbicides failed to protect women upon their exposure to hiv. a possible explanation for this failure is that sulfated oligo- and polysacch ... | 2016 | 26643323 |
| decrease in seroprevalence for herpesviruses among pregnant women in finland: cross-sectional study of three time points 1992, 2002 and 2012. | maternal herpesvirus infections during pregnancy may cause fetal and neonatal infections. we investigated the seroprevalence of five herpesviruses: cytomegalovirus (cmv), herpes simplex virus (hsv) 1 and 2, varicella zoster virus (vzv) and epstein-barr virus (ebv) in randomly selected samples from pregnant finnish women from the years 1992, 2002 and 2012. over 20 years, the seroprevalences decreased significantly for cmv from 84.5% to 71.5% (p = 0.007) and hsv-1 from 69.5% to 45% (p < 0.001). th ... | 2016 | 26654892 |
| higher throughput quantification of neutralizing antibody to herpes simplex viruses. | we report a rapid, higher throughput method for measuring neutralizing antibody to herpes simplex virus (hsv) in human sera. clinical isolates and sera from the herpevac trial for women were used in a colorimetric assay in which infection of tissue culture (lack of neutralization) was indicated by substrate metabolism by beta-galactosidase induced in the elvis cell line. the neutralization assay was optimized by addition of guinea pig complement, which particularly enhanced neutralizing antibody ... | 2015 | 26658766 |
| evaluation of an automated chemiluminescent immunoassay in typing detection of igg antibodies against herpes simplex virus. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) are common infectious agents worldwide and the primary infection of hsv remains a major problem in the pregnant women in china nowadays. at present, typing detection of hsv is mainly based on elisa in china. | 2016 | 26666528 |
| pericoital application of tenofovir gel reduced risk for herpes simplex virus type 2 in hiv-negative women. | 2015 | 26666808 | |
| antibodies are required for complete vaccine-induced protection against herpes simplex virus 2. | herpes simplex virus 2 (hsv-2) 0δnls is a live hsv-2 icp0- mutant vaccine strain that is profoundly attenuated in vivo due to its interferon-hypersensitivity. recipients of the hsv-2 0δnls vaccine are resistant to high-dose hsv-2 challenge as evidenced by profound reductions in challenge virus spread, shedding, disease and mortality. in the current study, we investigated the requirements for hsv-2 0δnls vaccine-induced protection. studies using (uv)-inactivated hsv-2 0δnls revealed that self-lim ... | 2015 | 26670699 |
| bacterial exopolysaccharide of shallow marine vent origin as agent in counteracting immune disorders induced by herpes virus. | herpes simplex virus type 2 (hsv-2) is responsible of the continuously increasing viral infections in humans. in a previous study we demonstrated that the exopolysaccharide produced by bacillus licheniformis strain b3-15 (eps-b3-15), was able to hinder the hsv-2 replication in peripheral blood mononuclear cells (pbmc) and this antiviral activity appear to be related to a significant stimulation of the th1-cytokines. in this study we analyse the role of eps-b3-15 on th2 cytokine production by pbm ... | 2016 | 26674976 |
| association of interferon lambda-1 with herpes simplex viruses-1 and -2, epstein-barr virus, and human cytomegalovirus in chronic periodontitis. | periodontal tissues facilitate the homing of herpes viruses that elicit the immune-inflammatory response releasing the interferons (ifn). ifn lambda-1 (λ1) can suppress the replication of viruses, and induces the antiviral mechanism. the aim of the present study was to evaluate the association between ifn-λ1 and periodontal herpes viruses in the immunoregulation of chronic periodontal disease. | 2015 | 26677065 |
| diversity and combinations of infectious agents in 38 adults with an infection-triggered reactive haemophagocytic syndrome: a multicenter study. | reactive haemophagocytic syndrome (hs) is a rare condition that occurs in patients with infections, haematological malignancies or autoimmune diseases. although various microorganisms are thought to trigger hs, most of the literature data on this topic have been gathered in single-centre case series. here, we sought to characterize infectious triggers in a large, multicentre cohort of patients with hs. patients were included in the present study if hs was solely due to one or more infections. de ... | 2016 | 26686809 |
| transient increase in herpes simplex virus type 2 (hsv-2)-associated genital ulcers following initiation of antiretroviral therapy in hiv/hsv-2-coinfected individuals. | immune reconstitution inflammatory syndrome (iris) in human immunodeficiency virus (hiv)-infected persons beginning antiretroviral therapy (art) has been incompletely characterized for herpes simplex virus type 2 (hsv-2). | 2016 | 26704611 |
| temporal cognitive decline associated with exposure to infectious agents in a population-based, aging cohort. | numerous cross-sectional studies have related exposure to neurotropic infectious agents with cognitive dysfunction in older adults, however, the temporal sequence is uncertain. | 2017 | 26710257 |
| development of a multiplex real-time pcr for the simultaneous detection of herpes simplex and varicella zoster viruses in cerebrospinal fluid and lesion swab specimens. | herpes simplex viruses (hsv) and varicella zoster virus (vzv) can have very similar and wide-ranging clinical presentations. rapid identification is necessary for timely antiviral therapy, especially with infections involving the central nervous system, neonates, and immunocompromised individuals. detection of hsv-1, hsv-2 and vzv was combined into one real-time pcr reaction utilizing hydrolysis probes. the assay was validated on the lightcycler(®) (roche) and applied biosystems 7500 real-time p ... | 2016 | 26711555 |
| differential mechanisms of tenofovir and tenofovir disoproxil fumarate cellular transport and implications for topical preexposure prophylaxis. | intravaginal rings releasing tenofovir (tfv) or its prodrug, tenofovir disoproxil fumarate (tdf), are being evaluated for hiv and herpes simplex virus (hsv) prevention. the current studies were designed to determine the mechanisms of drug accumulation in human vaginal and immune cells. the exposure of vaginal epithelial or t cells to equimolar concentrations of radiolabeled tdf resulted in over 10-fold higher intracellular drug levels than exposure to tfv. permeability studies demonstrated that ... | 2015 | 26711762 |
| herpes simplex type 2 encephalitis after craniotomy: case report and literature review. | herpes simplex encephalitis (hse) after neurosurgical procedures is extremely uncommon, and the few published case reports mainly described herpes simplex virus type 1 (hsv-1) as being culpable. we present a rare case of hsv-2 encephalitis after craniotomy and describe its pathophysiology and optimal management. | 2016 | 26724620 |
| chlamydial pre-infection protects from subsequent herpes simplex virus-2 challenge in a murine vaginal super-infection model. | chlamydia trachomatis and herpes simplex virus-2 (hsv-2) genital tract co-infections have been reported in humans and studied in vitro but the clinical consequences are unknown. limited epidemiologic evidence suggests that these co-infections could be more severe than single infections of either pathogen, but the host-pathogen interactions during co-infection remain uncharacterized. to determine whether disease progression and/or pathogen shedding differs between singly-infected and super-infect ... | 2016 | 26726882 |
| sustained high prevalence of viral hepatitis and sexually transmissible infections among female sex workers in china: a systematic review and meta-analysis. | the 1980's economic boom has been associated with a rapid expansion of china's sex industry over the past three decades. consequently, the spread of sexually transmitted infections (stis) and hepatitis infections among female sex workers (fsw) has become an important public health issue in china. this study identifies prevalence and risks of hepatitis and stis in chinese fsws. | 2016 | 26732281 |
| herpes simplex virus encephalitis in peru: a multicentre prospective study. | herpes simplex virus (hsv) is one of the most commonly identified infectious aetiologies of encephalitis in north america and europe. the epidemiology of encephalitis beyond these regions, however, is poorly defined. during 2009-2012 we enrolled 313 patients in a multicentre prospective study of encephalitis in peru, 45 (14·4%) of whom had confirmed hsv infection. of 38 patients with known hsv type, 84% had hsv-1 and 16% had hsv-2. patients with hsv infection were significantly more likely to pr ... | 2016 | 26733400 |
| comparison of follicular and luteal phase mucosal markers of hiv susceptibility in healthy women. | the purpose of this study was to evaluate differences in vaginal immune cell populations, vaginal tissue gene expression, antimicrobial activity of the cervicovaginal (cv) lavage (cvl), vaginal flora, and p24 antigen production from cv tissues after ex vivo human immunodeficiency virus (hiv) infection between follicular (fol) and luteal (lut) phases of the menstrual cycle. cv tissue biopsies, cv secretions, and blood samples were obtained as part of two longitudinal clinical trials of healthy wo ... | 2016 | 26750085 |
| oasl1 deficiency promotes antiviral protection against genital herpes simplex virus type 2 infection by enhancing type i interferon production. | type i interferon (ifn) interferes with virus replication, promotes antiviral responses, and controls innate and adaptive immune responses to certain viruses. recently, we reported that 2'-5' oligoadenylate synthetase-like 1 (oasl1) negatively regulates type i ifn production by inhibiting the translation of the type i ifn-regulating master transcription factor, irf7. notably, while oasl1-deficient mice induce robust production of type i ifn and are resistant to systemic viral infection, the effe ... | 2016 | 26750802 |
| false-negative type-specific glycoprotein g antibody responses in sti clinic patients with recurrent hsv-1 or hsv-2 dna positive genital herpes, the netherlands. | herpes simplex virus (hsv) type-discriminating antibody tests (glycoprotein g (gg) directed) are used to identify naïve persons and differentiate acute infections from recurrences. we studied test characteristics of three commercially available antibody tests in patients with recurrent (established by viral pcr tests) herpes simplex virus type 1 (hsv-1) or herpes simplex virus type 2 (hsv-2) genital herpes episodes. | 2016 | 26755775 |
| herpes simplex virus (hsv) modulation of staphylococcus aureus and candida albicans initiation of hela 299 cell-associated biofilm. | although herpes simplex virus type-1 (hsv-1), and type-2 (hsv-2), staphylococcus aureus and candida albicans co-habit the oral and genital mucosa, their interaction is poorly understood. we determined the effect hsv has on bacterial and/or fungal adherence, the initial step in biofilm formation. hela229 cells were infected with hsv-1 (kos) gl86 or hsv-2 (kos) 333gj (-) at a multiplicity of infection (moi) of 50 and 10. s. aureus (atcc 25923) and/or c. albicans (yeast forms or germ tube forms) we ... | 2016 | 26758707 |
| hiv/sexually transmitted infection prevention messaging: targeting root causes of sexual risk behavior. | sexual risk behaviors (srbs) often lead to sexually transmitted infections (sti), yet little is known about what drives srb and whether this differs by sex. | 2016 | 26760178 |
| in vitro and in vivo antiherpetic effects of (1r,2r)-1-(5'-methylful-3'-yl)propane-1,2,3-triol. | in this study, we demonstrated the in vitro and in vivo antiherpetic activities of a stable furan derivative, (1r,2r)-1-(5'-methylful-3'-yl)propane-1,2,3-triol (mfpt), which had originally been isolated from streptomyces sp. strain fv60. in the present study, we synthesized mfpt from (5-methylfuran-3-yl)methanol in 6 steps for use in the experiments. mfpt showed potent in vitro antiviral activities against two acyclovir (acv)-sensitive (kos and hf) strains and an acv-resistant (a4-3) strain of h ... | 2016 | 26763002 |
| etiologies and management of aseptic meningitis in patients admitted to an internal medicine department. | several studies have focused on the clinical and biological characteristics of meningitis in order to distinguish between bacterial and viral meningitis in the emergency setting. however, little is known about the etiologies and outcomes of aseptic meningitis in patients admitted to internal medicine.the aim of the study is to describe the etiologies, characteristics, and outcomes of aseptic meningitis with or without encephalitis in adults admitted to an internal medicine department.a retrospec ... | 2016 | 26765411 |
| development and evaluation of the quantitative real-time pcr assay in detection and typing of herpes simplex virus in swab specimens from patients with genital herpes. | genital herpes (gh), which is caused mainly by herpes simplex virus (hsv)-2 and hsv-1, remains a worldwide problem. laboratory confirmation of gh is important, particularly as there are other conditions which present similarly to gh, while atypical presentations of gh also occur. currently, virus culture is the classical method for diagnosis of gh, but it is time consuming and with low sensitivity. a major advance for diagnosis of gh is to use real-time polymerase chain reaction (pcr). in this s ... | 2015 | 26770492 |
| does place of service matter? a utilisation and cost analysis of sexually transmissible infection testing from 2012 claims data. | background in this study, a previous study on the utilisation and cost of sexually transmissible infection (sti) tests was augmented by focusing on outpatient place of service for the most utilised tests. | 2016 | 26774890 |
| fulminant hepatic and multiple organ failure following acute viral tonsillitis: a case report. | pyogenic tonsillitis may often be observed in the general western population. in severe cases, it may require antibiotic treatment or even hospitalization and often a prompt clinical response will be noted. here we present an unusual case of progressive multiple organ failure including fulminant liver failure following acute tonsillitis initially mistaken for "classic" pyogenic (that is bacterial) tonsillitis. | 2016 | 26785992 |
| synthetic α-hydroxytropolones inhibit replication of wild-type and acyclovir-resistant herpes simplex viruses. | herpes simplex virus 1 (hsv-1) and hsv-2 remain major human pathogens despite the development of anti-hsv therapeutics as some of the first antiviral drugs. current therapies are incompletely effective and frequently drive the evolution of drug-resistant mutants. we recently determined that certain natural troponoid compounds such as β-thujaplicinol readily suppress hsv-1 and hsv-2 replication. here, we screened 26 synthetic α-hydroxytropolones with the goals of determining a preliminary structu ... | 2016 | 26787704 |
| the influence of hormonal factors on the risk of developing cervical cancer and pre-cancer: results from the epic cohort. | in addition to hpv, high parity and hormonal contraceptives have been associated with cervical cancer (cc). however, most of the evidence comes from retrospective case-control studies. the aim of this study is to prospectively evaluate associations between hormonal factors and risk of developing cervical intraepithelial neoplasia grade 3 (cin3)/carcinoma in situ (cis) and invasive cervical cancer (icc). | 2016 | 26808155 |
| dysbiosis-induced il-33 contributes to impaired antiviral immunity in the genital mucosa. | commensal microbiota are well known to play an important role in antiviral immunity by providing immune inductive signals; however, the consequence of dysbiosis on antiviral immunity remains unclear. we demonstrate that dysbiosis caused by oral antibiotic treatment directly impairs antiviral immunity following viral infection of the vaginal mucosa. antibiotic-treated mice succumbed to mucosal herpes simplex virus type 2 infection more rapidly than water-fed mice, and also showed delayed viral cl ... | 2016 | 26811463 |
| exposure to perinatal infections and bipolar disorder: a systematic review. | bipolar disorder (bd) is a debilitating psychiatric disorder and a growing global public health issue. notwithstanding bd has been conceptualized as a neuroprogressive illness, there are some evidences to suggest a role for neurodevelopmental pathways in the patho-etiology of this disorder. evidences on the associations between perinatal infections and risk for bipolar disorder have been inconsistent across studies. here, we performed a systematic review of observational studies on the relations ... | 2016 | 26812921 |
| clinical trials using the shang ring device for male circumcision in africa: a review. | male circumcision (mc) reduces the risk of hiv and other sexually transmitted infections (stis), including human papillomavirus (hpv) and herpes simplex virus type 2 (hsv-2), and is recommended as an important component of a comprehensive package of hiv prevention interventions. while computer modeling has demonstrated that substantial reductions in hiv could be achieved with rapid scale-up of mc services in sub-saharan africa, scale-up has lagged due to scarcity of trained providers coupled wit ... | 2014 | 26816759 |
| [erythema exudativum multiforme--case report]. | erythema exudativum multiforme (em) is an acute mucocutaneous disease. etiology of this disease is not clear. 50% - 60% cases of em is induced by hsv infection (herpes simplex virus 1, herpes simplex virus 2). em lesions may appear as red macules, erosions, ulcerations and hemorrhagic crusts. the study describes the case of 22 - year - old male who had characteristic lesions for em after hsv infection. | 2015 | 26817344 |
| h2ax phosphorylation and dna damage kinase activity are dispensable for herpes simplex virus replication. | herpes simplex virus type 1 (hsv-1) can establish both lytic and latent infections in humans. the phosphorylation of histone h2ax, a common marker of dna damage, during lytic infection by hsv-1 is well established. however, the role(s) of h2ax phosphorylation in lytic infection remain unclear. | 2016 | 26817608 |
| incidence of co-infections of hiv, herpes simplex virus type 2 and syphilis in a large cohort of men who have sex with men in beijing, china. | the hiv-epidemic among msm in china has worsened. in this key population, prevalence of hsv-2 and syphilis infection and co-infection with hiv is high. | 2016 | 26820145 |
| high conservation of herpes simplex virus ul5/ul52 helicase-primase complex in the era of new antiviral therapies. | the emergence of herpes simplex virus (hsv) resistance to current antiviral drugs, that all target the viral dna polymerase, constitutes a major obstacle to antiviral treatment effectiveness of hsv infections, especially in immunocompromised patients. a novel and promising class of inhibitors of the hsv ul5/ul52 helicase-primase (hp) complex has been reported to hinder viral replication with a high potency. in this study, we describe the low natural polymorphism (interstrain identity >99.1% at b ... | 2016 | 26826441 |
| broad and potent antiviral activity of the nae inhibitor mln4924. | in terms of infected human individuals, herpesviruses range among the most successful virus families. subclinical herpesviral infections in healthy individuals contrast with life-threatening syndromes under immunocompromising and immunoimmature conditions. based on our finding that cytomegaloviruses interact with cullin roc ubiquitin ligases (crls) in the context of interferon antagonism, we systematically assessed viral dependency on crls by utilizing the drug mln4924. crl activity is regulated ... | 2016 | 26829401 |
| characterization of the c-terminal nuclease domain of herpes simplex virus pul15 as a target of nucleotidyltransferase inhibitors. | the natural product α-hydroxytropolones manicol and β-thujaplicinol inhibit replication of herpes simplex viruses 1 and 2 (hsv-1 and hsv-2, respectively) at nontoxic concentrations. because these were originally developed as divalent metal-sequestering inhibitors of the ribonuclease h activity of hiv-1 reverse transcriptase, α-hydroxytropolones likely target related hsv proteins of the nucleotidyltransferase (ntase) superfamily, which share an "rnase h-like" fold. one potential candidate is pul1 ... | 2016 | 26829613 |
| neonatal herpes simplex virus presenting with isolated liver failure. | disseminated neonatal herpes simplex virus infection usually presents with multi-organ involvement. untreated, this disease has a mortality rate of approximately 80%. here, we describe a well-appearing 3-week old infant with isolated compensated hepatic failure caused by hsv-2. | 2014 | 26839769 |
| mathematical modeling of herpes simplex virus-2 suppression with pritelivir predicts trial outcomes. | pharmacokinetic and pharmacodynamic models estimate the potency of antiviral agents but do not capture viral and immunologic factors that drive the natural dynamics of infection. we designed a mathematical model that synthesizes pharmacokinetics, pharmacodynamics, and viral pathogenesis concepts to simulate the activity of pritelivir, a dna helicase-primase inhibitor that targets herpes simplex virus. our simulations recapitulate detailed viral kinetic shedding features in five dosage arms of a ... | 2016 | 26843190 |
| the agma1 poly(amidoamine) inhibits the infectivity of herpes simplex virus in cell lines, in human cervicovaginal histocultures, and in vaginally infected mice. | the development of topical microbicides is a valid approach to protect the genital mucosa from sexually transmitted infections that cannot be contained with effective vaccination, like hsv and hiv infections. a suitable target of microbicides is the interaction between viral proteins and cell surface heparan sulfate proteoglycans (hspgs). agma1 is a prevailingly cationic agmatine-containing polyamidoamine polymer previously shown to inhibit hspgs dependent viruses, including hsv-1, hsv-2, and hp ... | 2016 | 26854390 |
| antiviral activity of hatay propolis against replication of herpes simplex virus type 1 and type 2. | background propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. in this study we aimed to investigate: i) the antiviral activities of hatay propolis samples against hsv-1 and hsv-2 in hep-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses. material and methods all experiments were carried out in hep-2 cell cultures. proliferation assays were performed in 24-well flat bottom microplates. w ... | 2016 | 26856414 |
| treatment of colon cancer with oncolytic herpes simplex virus in preclinical models. | cancer stem cells (cscs), which are a rare population in any type of cancer, including colon cancer, are tumorigenic and responsible for cancer recurrence and metastasis. cscs have been isolated from a number of different solid tumors recently, although the isolation of cscs in colon cancer is still challenging. we cultured colon cancer cells in stem cell medium to obtain colonosphere cells. these cells possessed the characteristics of cscs, with a high capacity of tumorigenicity, migration and ... | 2016 | 26871935 |
| bacterial vaginosis: a synthesis of the literature on etiology, prevalence, risk factors, and relationship with chlamydia and gonorrhea infections. | bacterial vaginosis (bv) is a common vaginal disorder in women of reproductive age. since the initial work of leopoldo in 1953 and gardner and dukes in 1955, researchers have not been able to identify the causative etiologic agent of bv. there is increasing evidence, however, that bv occurs when lactobacillus spp., the predominant species in healthy vaginal flora, are replaced by anaerobic bacteria, such as gardenella vaginalis, mobiluncus curtisii, m. mulieris, other anaerobic bacteria and/or m ... | 2016 | 26877884 |
| seroprevalence of cmv, hsv-2 and hbv among hiv-infected malawian children: a cross-sectional survey. | little is known about viral co-infections in african human immunodeficiency virus (hiv)-infected children. we examined the prevalence of seromarkers for cytomegalovirus (cmv), herpes simplex virus type 2 (hsv-2) and hepatitis b virus (hbv) infections among hiv-infected, antiretroviral treatment (art)-naïve children in lilongwe, malawi. | 2016 | 26884443 |
| parents' reactions to testing for herpes simplex virus type 2 as a biomarker of sexual activity in botswana junior secondary school students. | background use of sexual activity biomarkers in hiv prevention trials has been widely supported to validate self-reported data. when such trials involve minors, researchers may face challenges in obtaining parental buy-in, especially if return of results procedures uphold the confidentiality and privacy rights of minors and preclude parental access to test results. in preparation for a randomised controlled trial (rct) with junior secondary school (jss) students in botswana, a formative assessme ... | 2016 | 26886026 |
| absent anti-n-methyl-d-aspartate receptor nr1a antibodies in herpes simplex virus encephalitis and varicella zoster virus infections. | a 2012 report and subsequent case series described anti-n-methyl-d-aspartate receptor (nmdar) antibodies in patients during the acute phase and relapse of herpes simplex virus 1 (hsv1) encephalitis (hsv1e). however, the prevalence of this phenomenon is unknown and systematic studies on other viral infections of the nervous system are missing. | 2017 | 26887329 |
| evolution of rational vaccine designs for genital herpes immunotherapy. | immunotherapeutic vaccines have emerged as a novel treatment modality for genital herpes, a sexually transmitted disease mainly caused by herpes simplex virus type 2. the approaches to identify potential vaccine antigens have evolved from classic virus attenuation and characterization of antibody and t cell responses in exposed, but seronegative individuals, to systematic screens for novel t cell antigens. combined with implementation of novel vaccine concepts revolving around immune evasion and ... | 2016 | 26896782 |
| smart cup: a minimally-instrumented, smartphone-based point-of-care molecular diagnostic device. | nucleic acid amplification-based diagnostics offer rapid, sensitive, and specific means for detecting and monitoring the progression of infectious diseases. however, this method typically requires extensive sample preparation, expensive instruments, and trained personnel. all of which hinder its use in resource-limited settings, where many infectious diseases are endemic. here, we report on a simple, inexpensive, minimally-instrumented, smart cup platform for rapid, quantitative molecular diagno ... | 2016 | 26900258 |
| subclinical generation of acyclovir-resistant herpes simplex virus with mutation of homopolymeric guanosine strings during acyclovir therapy. | suppressive therapy in patients with genital herpes has been used in japan since 2006. susceptibility and resistance of herpes simplex virus (hsv)-2 to acyclovir were examined in genital isolates from patients receiving suppressive therapy and compared with those from those naïve to acyclovir and receiving episodic treatment with acyclovir. | 2016 | 26917345 |
| an empiric hiv risk scoring tool to predict hiv-1 acquisition in african women. | to develop and validate an hiv risk assessment tool to predict hiv acquisition among african women. | 2016 | 26918545 |
| complement opsonization promotes herpes simplex virus 2 infection of human dendritic cells. | herpes simplex virus 2 (hsv-2) is one of the most common sexually transmitted infections globally, with a very high prevalence in many countries. during hsv-2 infection, viral particles become coated with complement proteins and antibodies, both present in genital fluids, which could influence the activation of immune responses. in genital mucosa, the primary target cells for hsv-2 infection are epithelial cells, but resident immune cells, such as dendritic cells (dcs), are also infected. dcs ar ... | 2016 | 26937039 |
| pseudotumoral recto-sigmoid herpes simplex virus type 2 in an hiv-infected patient: dramatic improvement with thalidomide. | 2016 | 26946154 | |
| the association between cervical human papillomavirus infection and subsequent hiv acquisition in tanzanian and ugandan women: a nested case-control study. | this study was performed to analyze the associations between cervical human papillomavirus (hpv) infection and human immunodeficiency virus (hiv) acquisition, using cervical samples from previous studies in tanzania and uganda. | 2016 | 26951818 |
| seropositivity and determinants of immunoglobulin-g (igg) antibodies against herpes simplex virus (hsv) types -1 and -2 in pregnant women in port harcourt, nigeria. | this study is the first documented prevalence of igg antibody against hsv-1&-2 in port harcourt, nigeria and thus provides baseline data for future in-depth studies on hsv infection in south-south, nigeria. | 2015 | 26957960 |
| lymphocytic meningitis caused by herpes simplex virus type 2. | 2016 | 26964890 | |
| status of vaccine research and development of vaccines for herpes simplex virus. | herpes simplex virus type-1 (hsv-1) and -2 (hsv-2) are highly prevalent global pathogens which commonly cause recurrent oral and genital ulcerations. less common but more serious complications include meningitis, encephalitis, neonatal infection, and keratitis. hsv-2 infection is a significant driver of the hiv epidemic, increasing the risk of hiv acquisition 3 fold. as current control strategies for genital hsv-2 infection, including antiviral therapy and condom use, are only partially effectiv ... | 2016 | 26973067 |
| novel therapeutic investigational strategies to treat severe and disseminated hsv infections suggested by a deeper understanding of in vitro virus entry processes. | the global burden of herpes simplex virus (hsv) legitimates the critical need to develop new prevention strategies, such as drugs and vaccines that are able to fight either primary hsv infections or reactivations. moreover, the ever-growing number of patients receiving transplants increases the number of severe hsv infections that are unresponsive to current therapies. finally, the high global incidence of genital hsv-2 infection increases the risk of perinatal transmission to newborns, in which ... | 2016 | 26976690 |
| seroprevalence and risk factors of herpes simplex virus-2 among pregnant women attending antenatal care at health facilities in wolaita zone, ethiopia. | herpes simplex virus type-2 is the common cause of genital ulcer disease worldwide. genital herpes infection is a major concern in pregnancy due to the risk of neonatal transmission. | 2016 | 26979484 |
| accumulation of a soluble form of human nectin-2 is required for exerting the resistance against herpes simplex virus type 2 infection in transfected cells. | cell entry of herpes simplex virus type 2 (hsv-2) requires the interaction of viral glycoprotein d (gd) with the receptor nectin-1 and herpesvirus entry mediator (hvem). in addition, it is known that nectin-2 is also functional as a receptor for hsv-2, although the binding to the gd is weak. to examine an antiviral potential of a soluble form of human nectin-2 (hnectin-2ig), transfected vero cells expressing the entire ectodomain of nectin-2 fused to the fc portion of human igg were established. ... | 2016 | 26982466 |
| food insecurity is associated with hiv, sexually transmitted infections and drug use among men in the united states. | to examine the population-level association between food insecurity, hiv risk factors, and hiv serostatus among men, the group representing the majority of hiv diagnoses in the united states. | 2016 | 26990632 |
| alphaherpesvirus subversion of stress-induced translational arrest. | in this article, we provide an overview of translational arrest in eukaryotic cells in response to stress and the tactics used specifically by alphaherpesviruses to overcome translational arrest. one consequence of translational arrest is the formation of cytoplasmic compartments called stress granules (sgs). many viruses target sgs for disruption and/or modification, including the alphaherpesvirus herpes simplex virus type 2 (hsv-2). recently, it was discovered that hsv-2 disrupts sg formation ... | 2016 | 26999187 |
| in vitro studies of the antiherpetic effect of photodynamic therapy. | the number of viral infection cases in the department of gynecology and obstetrics has tended to increase over last few years. viruses form herpesvirus and cytomegalovirus families are associated with an increased risk for recurrent pregnancy loss. photodynamic therapy (pdt) is a promising new approach to treat viral infections in which viral particles are inactivated. it exhibits great therapeutic potential, particularly among this group of patients. this study examined the use of pdt to treat ... | 2016 | 27003896 |
| regulatory t cells are essential to promote proper cd4 t-cell priming upon mucosal infection. | regulatory t cells (tregs) limit autoimmunity and immunopathology using a variety of suppressive mechanisms, but their roles during pathogen-directed immune responses remain unclear. following herpes simplex virus-2 (hsv-2) infection, mice lacking tregs fail to control viral replication, pointing to a role for tregs in facilitating productive immune responses. using adoptive transfer of t-cell receptor transgenic cd4 t cells into treg-sufficient or treg-depleted mice prior to hsv-2 infection, we ... | 2016 | 27007674 |
| medroxyprogesterone acetate and levonorgestrel increase genital mucosal permeability and enhance susceptibility to genital herpes simplex virus type 2 infection. | depot-medroxyprogesterone acetate (dmpa) is a hormonal contraceptive especially popular in areas with high prevalence of hiv and other sexually transmitted infections (sti). although observational studies identify dmpa as an important sti risk factor, mechanisms underlying this connection are undefined. levonorgestrel (lng) is another progestin used for hormonal contraception, but its effect on sti susceptibility is much less explored. using a mouse model of genital herpes simplex virus type 2 ( ... | 2016 | 27007679 |
| conserved tryptophan motifs in the large tegument protein pul36 are required for efficient secondary envelopment of herpes simplex virus capsids. | herpes simplex virus (hsv) replicates in the skin and mucous membranes, and initiates lytic or latent infections in sensory neurons. assembly of progeny virions depends on the essential large tegument protein pul36 of 3,164 amino acid residues that links the capsids to the tegument proteins pul37 and vp16. of the 32 tryptophans of hsv-1-pul36, the tryptophan-acidic motifs (1766)wd(1767) and (1862)we(1863) are conserved in all hsv-1 and hsv-2 isolates. here, we characterized the role of these mot ... | 2016 | 27009950 |
| human herpesviruses as copathogens of hiv infection, their role in hiv transmission, and disease progression. | of eight human herpesviruses (hhvs), often, only herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) find mention in medical literature as both of these viruses are commonly associated with genital lesions and oral ulcers, commonly known as cold sores. however, role of human herpesviruses as copathogens and in aggravation and in the transmission of other human diseases, especially the acquired immunodeficiency syndrome (hiv/aids) has only very recently been recognized. therefore, screening and tr ... | 2017 | 27013807 |
| the epidemiology of herpes simplex virus type 2 infections in a large cohort of hiv-infected patients, 2006-2014. | 2016 | 27030927 | |
| no evidence of pritelivir resistance among herpes simplex virus type 2 isolates after 4 weeks of daily therapy. | pritelivir is a novel helicase-primase inhibitor in clinical development for treatment of herpes simplex virus type 2 (hsv-2) infections. in preclinical work, resistance-mediating mutations were identified in the hsv-2 genome at 3 loci in the ul5 gene and 1 locus in ul52. | 2016 | 27056950 |