Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| mode of action of phosphonoformate as an anti-herpes simplex virus agent. | phosphonoformate inhibited the replication of herpes simplex virus (hsv) type 1 and type 2 in culture. the concentration required to inhibit the replication of both types of virus by 2 logs at 28 h post-infection was approximately 150 microm. it was more potent than phosphonoacetate against the growth of both virus types. a virus mutant which is resistant to phosphonoacetate was cross-resistant to phosphonoformate. arsonoacetate, at 300 microm, had no antivirus activity. phosphonoformate also in ... | 1981 | 6260189 |
| parameters distinguishing herpes simplex virus type 2-transformed tumorigenic and nontumorigenic rat cells. | 1981 | 6256070 | |
| herpesviruses and parkinsonism. herpes simplex virus types 1 and 2, and cytomegalovirus antibodies in serum and csf. | antibodies against herpes simplex virus (hsv) types 1 and 2 and cytomegalovirus (cmv) were assayed with a microindirect hemagglutination (iha) test in the serum of 67 pairs of patients with parkinson's disease and controls. cerebrospinal fluid from 30 pairs was assayed. all patient and control serum was tested with a radioimmunoassay (ria) for antibodies against hsv type 1 subunit antigens. serum iha antibody level against hsv type 1 was increased in patients with parkinson's disease and ria ant ... | 1981 | 6257211 |
| immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper. | in this paper we report that viral polypeptides from herpes simplex virus 1 (hsv-1) and 2 (hsv-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. the polyvalent hyperimmune sera against hsv-1 reacted with 17 hsv-1 polypeptide bands and 8 hsv-2 polypeptide bands. concordantly, polyvalent sera against hsv-2 ... | 1981 | 6260673 |
| molecular genetics of herpes simplex virus. vii. characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in dna synthesis and accumulation of gamma polypeptides. | we report on the properties of a temperature-sensitive mutant produced by transfection of cells with intact dna and a specific dna fragment mutagenized with low levels of hydroxylamine. the plating efficiency of the mutant at 39 degrees c relative to that at 33.5 degrees c was 5 x 10(-6). the pattern of polypeptides produced at the nonpermissive temperature was similar to that seen with wild-type virus in infected cells treated with inhibitory concentrations of phosphonoacetic acid in that alpha ... | 1981 | 6260973 |
| repair replication of viral and cellular dna in herpes simplex virus type 2-infected human embryonic and xeroderma pigmentosum cells. | 1981 | 6261452 | |
| assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera. | a reliable and reproducible method for determining specific reactivity to herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in human sera has been developed. human sera were used to immunoprecipitate hsv-specific glycoprotein antigens from both hsv-1- and hsv-2-infected cell extracts. the viral glycoproteins precipitated from these extracts were then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to detect specific reactivity of the sera with distinct type-specific anti ... | 1981 | 6262247 |
| production of hybrid cell lines secreting antibodies to herpes simplex virus type 2. | following different immunization schedules we have produced 102 myeloma--spleen cell hybrid lines (30.8% of the total) secreting antibodies to hsv-2 infected cells. the highest yield of hybrids was derived from fusion of myeloma cells with spleen cells from mice sensitized by footpad injection of formalin-inactivated virus and boosted intravenously with the same antigen. to date, twelve of the antibody-producing hybrids have been cloned by limiting dilutions to generate over 100 monoclonal lines ... | 1981 | 6262346 |
| involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2. | we have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (hsv-2) replication can be induced by human cytomegalovirus (hcmv). the mechanism of this reactivation of quiescent hsv-2 by hcmv has been further defined. the hcmv function(s) responsible for hsv-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing hsv-2 after superinfection was determined. using independent biological, genetic and molecular t ... | 1981 | 6262523 |
| aminoacyl fucosides as possible biochemical markers at tumorigenic and metastatic potential in herpes simplex virus type 2-transformed rat cells. | two classes of aminoacyl fucosides termed fl3 and fl4 were studied as possible markers of tumorigenic and metastatic potential in herpes simplex virus type 2 transformed rat cells. in the present study, clonal cell lines of transformed highly tumorigenic and metastatic (t-ref-g-1.1), weakly tumorigenic and nonmetastatic (t-ref-g-2.1), nontumorigenic (t-ref-g-2.0), and secondary nontransformed rat embryo fibroblast cells were labeled with [3h]fucose, and cell extracts were analyzed for ratio of r ... | 1981 | 6266657 |
| herpes virus inactivation by chemical carcinogens: differential inactivation of herpes simplex viruses by 4-nitroquinoline 1-oxide and related compounds. | treatment of stocks of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with the chemical carcinogen 4-nitroquinoline 1-oxide (nqo) resulted in inactivation of virus infectivity at rates which were directly dependent on the concentration of nqo and interval of exposure to nqo. hsv-1 strains were more sensitive than hsv-2 strains to inactivation by nqo, although survival curves of both hsv types were multicomponent. exposure of hsv-2 to a related group of chemicals suggested that the struct ... | 1981 | 6266824 |
| complement-fixing antibody to the ag-4 antigen in herpes simplex virus type 2-infected patients. | sera collected from confirmed herpes simplex virus type 2 (hsv-2) patients were found to be devoid of complement-fixing antibody to the ag-4 antigen at the time of the herpes lesion outbreak in 10 out of 13 cases. however, 1 to 4 weeks after hsv-2 lesion appearance, 28 out of 30 patients acquired complement-fixing antibody to the ag-4 antigen. the sera of these patients contained immunoglobulin m antibody activity and the ability to immunoprecipitate a 160,000-molecular weight early hsv-2 antige ... | 1981 | 6266964 |
| latency in vitro using irradiated herpes simplex virus. | human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ... | 1981 | 6267167 |
| physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue. | the physical mapping of six ts mutations of herpes simplex virus type 2 (hsv-2) is presented. the results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 hsv-1/hsv-2 intertypic recombinants. the order of these mutations on the physical map of hsv-2 is unambiguous and correlates almost exactly with the previously published genetic map of timbury & calder (1976). one of the mutants studied (hsv-2 ts12) has apparently two distinct conditionally lethal ts m ... | 1981 | 6267168 |
| experimental studies of acute and recurrent herpes simplex virus infections in the murine heart and dorsal root ganglia. | the multiplication of hsv-1 and hsv-2 strains in the heart and the corresponding dorsal root ganglia (drg) was examined in experimentally infected mice. infectious hsv-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the drg. both the heart and drg yielded infectious hsv-2 from the fourth to the twenty-first day after inoculation. the hsv-2, but not the hsv-1, induced recrudescent disease in chronically infected mice up to 18 months after i ... | 1981 | 6267190 |
| [new antigens induced by herpes simplex virus in human tumors]. | tissues of malignant tumors of the genitalia (cervix, uterus body, ovary) contain a specific antigen associated with herpes simplex virus type 2 (hsv-2) identical with the antigen of the infected cells. the virus-induced antigen was detected in tissues of cervical carcinoma in 35% of cases, in 15% of tumors of the corpus uteri and in 13% of ovary tumors. hsv-2 was isolated from pathologically altered cervical carcinoma cells in 2 out of 56 cases examined. these facts indicate the presence of hsv ... | 1981 | 6267821 |
| differentiation of members of the human herpesviridae family by radioimmunoassay. | many individuals who are seronegative for one member of the human herpesviridae family are strongly seropositive for other members. using sera from such individuals, the radioimmunoassay technique demonstrated absence of antigen-antibody cross-reactions between varicella-zoster virus (vzv) and herpes simplex virus (hsv) at levels of less than one part in 1,000. sera containing antibody to both hsv and vzv were absorbed with antigens of one agent without significantly altering the amount of remai ... | 1981 | 6268545 |
| protectivity of herpes simplex virus antigens: studies in mice on the adjuvant effect of piclc and on the dependence of protection on t cell competence. | the efficacy of a herpes simplex virus type 1 (hsv-1) envelope antigen (eag) preparation against hsv infection was studied in t cell competent and t cell deficient mice. immuno-competent mice were successfully protected against herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a hsv-1 envelope antigen (hsv-1 eag) preparation. since hsv-1 eag was considerably less effective than the who ... | 1981 | 6268955 |
| the influence of different modes of immunization on the experimental genital herpes simplex virus infection of mice. | previous investigations, which simulated the usual sequence of the human herpes simplex virus (hsv) infections, had shown that the oral infection of mice with hsv-1 caused only weak protection from genital infection with hsv-2, although the course of infection was attenuated and lethality diminished. this heterologous, heterotopic model was compared with a homologous, heterotopic and a heterologous, homotopic model. the results did not differ very much, although the homologous immunization prote ... | 1981 | 6268958 |
| antiherpesviral and anticellular effects of 1-beta-d-arabinofuranosyl-e-5-(2-halogenovinyl) uracils. | 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl) uracil (bv-ara-u) and 1-beta-d-arabinofuranosyl-e-5-(2-chlorovinyl)uracil (cv-ara-u) were tested for their anti-herpesviral activity in virus rating method, a plaque reduction method, and a virus yield reduction method, using human embryonic lung fibroblast (hel-f) cells, at a concentration as low as 0.1 microgram/ml, both drugs exerted a marked inhibitory effect on the development of cytopathogenic effect induced by herpes simplex virus type 1 (hsv-1 ... | 1981 | 6269482 |
| induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2. | a series of studies were performed to evaluate the oncogenic potential of inactivated herpes simplex viruses types 1 (hsv-1) and 2 (hsv-2) in the mouse cervix. hsv-1 or hsv-2 prepared in hep-2 cell cultures and inactivated by exposure to formalin or ultraviolet light was applied to the mouse cervix for periods ranging from 20 to 90 weeks. control mice were exposed for the same period to control fluids. vaginal cytologic preparations from all animals were examined weekly to detect epithelial abno ... | 1981 | 6269724 |
| recovery of mice from herpes simplex virus type 2 hepatitis: adoptive transfer of recovery with immune spleen cells. | young balb/c mice inoculated intraperitoneally with herpes simplex virus type 2 develop focal necrotizing hepatitis. after infection, the livers of these mice show increasing virus titers, which reach a maximum on day 3 after infection; this is followed by a dramatic decrease in the amount of virus recovered on days 4 and 5. this decrease in virus content is accompanied by a progressive infiltration of the lesions with mononuclear leukocytes and an apparent resolution of the lesions. adoptive tr ... | 1981 | 6269998 |
| conversion of herpetic lesions to malignancy by ultraviolet exposure and promoter application. | many lines of evidence exist associating herpes simplex virus (hsv) with the development of carcinoma, but much of this evidence is anecdotal or associative in nature and does not prove a cause and effect. the purpose of this research was to investigate the oncogenic potential of hsv type 2 (hsv-2) in vivo. a mouse model for lip carcinogenesis was designed to combine hsv-2 infection, u.v. exposure and tetradecanoyl-phorbol-acetate (tpa) application. hsv-2 inoculation on to abraded mouse lips was ... | 1981 | 6270265 |
| nucleotide sequences of the joint between the l and s segments of herpes simplex virus types 1 and 2. | the a sequence of herpes simplex virus (hsv) is present as a direct repeat at the genomic termini and also in inverted orientation at the joint between the l and s segments. dna sequences have been determined for the joint regions of the genomes of hsv-1 and hsv-2, and relative to these sequences the genomic termini are in both cases located close to a short direct repeat of 17 to 21 base pairs (bp) at the b-a and a-c junctions. the hsv-1 joint region contains three separate tandem direct reiter ... | 1981 | 6270266 |
| physical mapping of drug resistance mutations defines an active center of the herpes simplex virus dna polymerase enzyme. | the genome structures of herpes simplex virus type 1 (hsv-1)/hsv-2 intertypic recombinants have been previously determined by restriction endonuclease analysis, and these recombinants and their parental strains have been employed to demonstrate that mutations within the hsv dna polymerase locus induce an altered hsv dna polymerase activity, exhibiting resistance to three inhibitors of dna polymerase. the viral dna polymerases induced by two recombinants and their parental strains were purified a ... | 1981 | 6270349 |
| nonstructural proteins of herpes simplex virus. ii. major virus-specific dna-binding protein. | the major herpes simplex virus type 2 dna-binding infected cell-specific polypeptides 11 and 12 have been purified to homogeneity from extracts of virus-infected cells. monospecific antiserum to the purified protein has been made and used to examine virus temperature-sensitive mutants for defects in the synthesis of the protein and to probe virus dna synthesis in isolated chromatin. the purified protein acted directly on a polydeoxyadenylic acid-polydeoxythymidylic acid helix, reducing its melti ... | 1981 | 6270358 |
| identification of a virus-specific polypeptide associated with a transforming fragment (bglii-n) of herpes simplex virus type 2 dna. | the bglii n fragment of herpes simplex virus type 2 (hsv-2) dna (approximately 0.58 to 0.63 map unit) was examined for encoded products. using plasmid pgz59, which consists of bglii-n cloned in pat153, in conjunction with hybrid arrested translation, mrna selection, and in vitro protein synthesis, we found that the major translated product of this region has an approximate molecular weight of 37,800. by further mapping, coding sequences for this polypeptide were located within the region of bgli ... | 1981 | 6270369 |
| herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication. | in addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ... | 1981 | 6270377 |
| focus formation and neoplastic transformation by herpes simplex virus type 2 inactivated intracellularly by 5-bromo-2'-deoxyuridine and near uv light. | the induction of focus formation in low serum and of neoplastic transformation of syrian hamster embryo cells was examined after the expression of herpes simplex virus type 2 functions. syrian hamster embryo cells infected at a high multiplicity (5 pfu/cell) with 5-bromo-2'-deoxyuridine-labeled herpes simplex virus type 2 (11% substitution of thymidine residues) were exposed to near uv light irradiation at various times postinfection. this procedure specifically inactivated the viral genome, whi ... | 1981 | 6270382 |
| phospholipid synthesis in human embryo fibroblasts infected with herpes simplex virus type 2. | the effect of herpes simplex virus type 2 infection on the synthesis of phospholipids in human embryo fibroblasts was determined at temperatures permissive (35 c) or nonpermissive (42 c) for virus replication. incorporation of [32p]i was decreased by herpes simplex virus type 2 infection after 6 hr, which corresponds to the time of initiation of progeny virus production. no differences were observed in the relative incorporation of [32p]i phospholipid classes. in another series of experiments ce ... | 1981 | 6270493 |
| in vitro studies of a co-carcinogenic effect of vaccinia and herpes group viruses. | the results of studies of a co-carcinogenic effect of two human infectious viruses in tissue culture are reported here. viable vaccinia virus actively replicating in the cells of primary balb/c tissue culture and in a number of continuous murine cell lines has been shown to induce in them expression of major structural p30 protein of murine retroviruses. vaccinia virus has been also shown to cause biochemical transformation of murine cells. evidence for the capacity of herpes simplex virus type ... | 1981 | 6270580 |
| herpes simplex virus type 2 and cervical cancer. | 1981 | 6271502 | |
| specific lymphocyte blastogenic responses in children with cytomegalovirus and herpes simplex virus infections acquired early in infancy. | cell-mediated immune responses in 27 infants and children with cytomegalovirus (cmv) infection acquired between birth and 1 year of age were compared with responses in 13 children who had neonatal herpes simplex virus (hsv) infection. infection was asymptomatic in 25 of 27 cmv-infected children; the 13 patients with hsv infection were all ill as newborns. the median age when studied was 46 months for children infected with cmv and 24 months for those infected with hsv. we measured lymphocyte tra ... | 1981 | 6271679 |
| use of monoclonal antibody directed against herpes simplex virus glycoproteins to protect mice against acute virus-induced neurological disease. | monoclonal antibodies hcl and hd1, directed against herpes simplex virus type 1 (hsv-1) glycoproteins gc and gd, respectively, were evaluated for their ability to passively immunize mice against acute virus-induced neurological disease after footpad inoculation with hsv-1 or herpes simplex virus type 2 (hsv-2). control virus-infected mice receiving a single intraperitoneal injection of normal serum died within 7 to 10 days after the spread of virus from footpad to spinal cord and brain. however, ... | 1981 | 6271681 |
| ocular lesions associated with dissemination of type 2 herpes simplex virus from skin infection in newborn rabbits. | the subcutaneous inoculation of the backs of new zealand white rabbits 17 to 34 hr old with 10(3) 50% tissue culture infection dose (tcid50) of type 2 herpes simplex virus (hsv-2) induced cutaneous lesions within 24 hr, foci of disseminated infection in many organs (including the eye) on day 3 and thereafter, and the death of the animals on day 5 with infection of the central nervous system. infectious hsv-2 could be isolated from the mononuclear cells and plasma of the peripheral blood, indicat ... | 1981 | 6271702 |
| the detection of dna tumour virus-specific rna sequences in abnormal human cervical biopsies by in situ hybridization. | we have used the technique of in situ nucleic acid hybridization and autoradiography of thin frozen sections of human tissue to search for virus rna sequences in human cervical tumours. of cervical biopsies with abnormal cytology, 67% bound herpes simplex virus type 2 (hsv2) 3h-labelled dna probes and 39% bound adenovirus type 2 (ad2) 3h-labelled dna probes, whereas control experiments with phage lambda 3h-labelled dna probes, under the same conditions, bound to only 7% of cases. in contrast, no ... | 1981 | 6271899 |
| one functional copy of the long terminal repeat gene specifying the immediate-early polypeptide ie 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus. | the hsv-1/hsv-2 intertypic recombinant bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (irl and trl) and expresses both the immediate-early polypeptide ie 110 of hsv-1 and its functionally equivalent polypeptide ie 118 of hsv-2. the genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. subclone 14 lacked most of the irl sequence, including the region from which part of the mrna ... | 1981 | 6271901 |
| differential immunologic reactivity and processing of glycoproteins ga and gb of herpes simplex virus types 1 and 2 made in vero and hep-2 cells. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) specify five major glycoproteins designated as ga, gb, gc, gd, and ge. previous studies have shown that ga and gb differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. moreover, ga and gb of hsv-1 crossreact in serologic tests with the corresponding glycoproteins of hsv-2. in this paper, we report on the reactivities of ga and gb of hsv-1 and hsv-2 with 24 independently de ... | 1981 | 6272294 |
| consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus. | electron microscopic examination showed microtubular structures in fl cells infected with all 18 strains of hsv-2 examined, but not in cells infected with 9 strains of hsv-1. these structures were also detected in other cultured cells (vero and earle's l cells) infected with hsv-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (ddd strain) infected with this type of virus. thus the microtubules were consistently detected in ... | 1981 | 6272691 |
| "the herpesvirus hypothesis"--are koch's postulates satisfied? | 1981 | 6273266 | |
| [studies on the nature of tubular structures as a marker of herpes simplex virus type 2 (author's transl)]. | 1981 | 6273274 | |
| kaposi's sarcoma. iv. detection of cmv dna, cmv rna and cmna in tumor biopsies. | in order to determine whether human cytomegalovirus- (cmv) dna homologous sequences as well as cmv-specific rna(s) and antigen(s) exist in tumor biopsies of kaposi's sarcoma (ks) dna-dna reassociation, rna-dna in situ cytohybridization and anticomplement immunofluorescence test (acif) tests were applied. three of 10 dnas extracted from kaposi sarcoma biopsies contained dna sequences homologous to radioactively labelled human cmv dna probe. the amount of cmv dna in these sarcoma tissues was calcu ... | 1981 | 6273333 |
| evaluation of the antiherpetic activity of 2'-fluoro-5-iodo-ara-c in rabbit eyes and cell cultures. | 2-fluoro-5-iodo-ara-c (fiac), a new and potent drug, was tested for antiviral activity against several strains of herpes simplex virus (hsv), types 1 and 2. effective dose-50% (ed-50) determinations for fiac ranged from 0.023 to 0.51 mum for hsv-2. fiac-treated cells did not exhibit any toxicity until the drug concentration was increased 2000-fold above the ed-50 level. ocular herpetic keratitis in new zealand white rabbits was treated with 1.0%, 0.1%, 0.01% fiac beginning 3 days after inoculati ... | 1981 | 6273355 |
| differences in neurovirulence among isolates of herpes simplex virus types 1 and 2 in mice using four routes of infection. | differences in neurovirulence between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using recent clinical isolates and laboratory-passaged strains in intravaginal, intranasal, intraperitoneal, and intracerebral infections of mice. the hsv-2 isolates caused higher death rates in all four infections. no differences in death rate were observed between recent and passaged isolates of either hsv-1 or hsv-2. after intravaginal inoculation, hsv-1 isolates replicated to higher ... | 1981 | 6273475 |
| repression and activation of the genome of herpes simplex viruses in human cells. | we have described previously a cell culture system in which the herpes simplex virus (hsv) type 2 (hsv-2) genome is maintained in a repressed form after treatment of infected cells with 1-beta-d-arabinofuranosylcytosine and increase of incubation temperature from 37 degrees c to 39.5 degrees c. infectious hsv-2 production was activated by altering incubation temperature or by superinfecting with human cytomegalovirus. we now report the establishment of an analogous system utilizing hsv type 1 (h ... | 1981 | 6273875 |
| [possible role of herpes simplex virus type 2 in the etiology of cervical cancer]. | 1981 | 6274212 | |
| inability to rescue viral genes from human cells biochemically transformed by herpes simplex virus type 2 dna. | 1981 | 6274496 | |
| molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection. | in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ... | 1981 | 6275122 |
| [experimental endophthalmitis induced by herpes simplex virus. 2. intracarotid inoculation (author's transl)]. | 1981 | 6275689 | |
| the development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses. | two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of mrc-5 and vero cells, and herpes simplex type 2 virus. cell growth was similar in both systems giving yields of 14 x 10(5)/cm2 in microcarrier and 18 x 10(5)/cm2 in glass sphere cultures with yields of vero cells being marginally better than mrc-5 cells. virus yields were only slightly lower in these cultures than in small scale stationary cultures ... | 1981 | 6281104 |
| herpes simplex vaccine using cell membrane associated antigen in an animal model. | vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of herpes simplex virus type 2 (hsv-2) infected vero cells with dithiothreitol and formaldehyde. these preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with hsv-2. | 1981 | 6281105 |
| expression of herpes simplex virus common surface antigens and malignancy by clonal cells of a herpes simplex virus type 2-transformed line. | herpes simplex virus (hsv) type common surface antigens (csa) were examined by indirect immunofluorescence with rabbit antiserum to hsv type 1 in a clonal hamster cell line 155-4 transformed by hsv type 2. the tumor formation was examined in hamsters transplanted with various transformed and tumor cells. the examination of subclones derived from 1554-cell line gave the following results. (1) thirty subclones were isolated and classified into three phenotypes as to csa expression: (i) in csa-pos ... | 1981 | 6281115 |
| antiherpes activity of [e]-5-(1-propenyl)-2'-deoxyuridine and 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil. | 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil has been synthesized, and this compound and [e]-5-(-propenyl)-2'-deoxyuridine have been tested for inhibition of herpes virus multiplication. only [e]-5-(1-propenyl)-2'-deoxyuridine was found to be an active inhibitor reducing by 50% the plaque formation of herpes simplex virus type 1 (hsv-1) at about 1 mum. a comparison to the bromovinyl derivatives showed the following order of descending activity; [e]-5-(2-bromovinyl)-2'-deoxyuridine greater than ... | 1981 | 6280606 |
| a comparison of phosphonoacetic acid and phosphonoformic acid activity in genital herpes simplex virus type 1 and type 2 infections of mice. | the activity of phosphonoacetic acid (paa) and phosphonoformic acid (pfa) against four strains of herpes simplex virus type 1 (hsv-1) and four strains of hsv-2 were compared in tissue culture and in a murine model of genital herpes. in mouse embryo fibroblast cells, both drugs were three-fold more active against the hsv-1 strains than against the hsv-2 strains. in contrast, in the animal model infections, paa appeared to be more active against the hsv-2 strains, while pfa was equally effective a ... | 1981 | 6280607 |
| roles of polyamines in the replication of animal viruses. | several animal viruses are known to contain significant amounts of polyamines but so far the function of these viral components is poorly understood. in this study the role of polyamines in the replication of two different types of viruses, herpes simplex virus type 2 and semliki forest virus (sfv) has been investigated. purified sfv was found to contain fairly small amounts of polyamines, sufficient to neutralize only about 3% of viral nucleic acid phosphate, i.e., 1/20 of that found in herpes ... | 1981 | 6279979 |
| [use of a contraceptive suppository as chemoprophylaxis against sexually-transmitted diseases]. | the incidence of sexually transmitted diseases (std), especially gonorrhea, has risen dramatically in the past 2 decades. the population at greatest risk is also at high risk for unwanted pregnancies. since currently employed treatment methods are failing to curb the problem, a search was instituted for an available vaginal contraceptive that would have a prophylactic effect against these diseases. in vitro studies have indicated that a vaginal contraceptive made of phenylmercuric acetate is ... | 1981 | 6277015 |
| sexually transmitted infections and cervical atypia. | epidemiologic studies have long associated cervical cancer and dysplasia with sexual activity. in the past two decades an abundance of biological and epidemiologic data have indicated a relationship between herpes simplex virus type 2 (hsv-2) and these conditions. unfortunately, the strength of the evidence (albeit circumstantial) has led many researchers to focus exclusively on the putative role of hsv-2 as the carcinogen. at the same time, many studies have shown that cervical atypia, if it is ... | 1981 | 6277023 |
| [new antigen induced by herpes simplex virus in human tumors]. | the study showed the new antigen induced by herpes simplex virus type 2 (hsv-2) to be present not only in cancer tumors of the cervix and corpus uteri, and ovaries but also in malignant tumors of the mammary glands, kidneys, urinary bladder, as well as in tissues of fibrous-cystic mastopathy and fibroadenoma of the mammary glands. in malignant tumors of the cervix, however, the hsv-2-induced antigen was found more frequently and had a higher serological activity than in other tumors. in contrast ... | 1981 | 6278776 |
| [malignant transformation of newborn hamster cells by herpes simplex virus type 2]. | the oncogenic potentials of hsv-2 inactivated by ultraviolet rays in newborn hamster cell culture were studied. virus-induced morphological and malignant transformation of cells was accompanied by synthesis of virus-specific antigen, changes in morphology, formation of colonies in semi-liquid agar, and tumorigenicity of cells. the animals bearing tumors induced by transformed cells showed humoral and cellular immune responses to the virus-specific antigen. lines of transformed and tumor cells we ... | 1981 | 6278782 |
| acyclovir therapy of neonatal herpes simplex virus type 2 infections in rabbits. | new zealand white rabbits less than 30 h old were inoculated subcutaneously with 10(3) 50% tissue culture infectious doses of type 2 herpes simplex virus. the animals were randomly assigned to a treatment schedule of daily intraperitoneal injections of acyclovir, beginning on the day of virus inoculation for 6 or 12 days, on post-inoculation day 1 for 6 days, or on post-inoculation day 2 for 6 days. the acyclovir was given in doses of 50 mg/kg of body weight per day. similarly infected animals r ... | 1981 | 6282195 |
| viruses and gynecologic cancers: herpesvirus protein (icp 10/ag-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity. | the studies associating infections by herpes simplex virus type 2 (hsv-2) with carcinoma of the human uterine cervix are reviewed within the context of three possible interpretations. extensive seroepidemiologic evidence indicates that the virus does not grow preferentially in neoplastic tissue, nor is the association of hsv-2 with cervical carcinoma a reflection of their independent relationship to promiscuity. while a number of infectious agents, including other viruses, are associated with ce ... | 1981 | 7023662 |
| herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection. | women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ... | 1981 | 6941038 |
| effect of herpes simplex virus type 2 and friend erythroleukemia virus infection of ige antibody responses to ascaris antigen in mice. | the ige response to ascaris antigen, as measured by passive cutaneous anaphylactic reactions, was suppressed in mice infected with either herpes simplex virus type 2 (hsv-2) or friend leukemia virus (fv). fv was not suppressive unless infection preceded immunization whereas hsv-2 infection was immunosuppressive before or after immunization. however, the suppression by hsv-2 was relatively short-lived. | 1981 | 7228435 |
| [studies on the placental damage and iugr caused by intravaginally inoculated herpes simplex virus type-2 (hsv-2) during pregnancy (author's transl)]. | it has been recognized that intrauterine growth retardation (iugr) could be caused by viral infection during pregnancy. we have experimentally attempted the intravaginal inoculation of hsv-2 (uw-268) 1.2-2.0 x 10(6) plaque forming unit in the pregnant rat. hsv-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. the invading route of the virus was investigate ... | 1981 | 7234355 |
| antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin infection. | win 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ... | 1981 | 7247370 |
| herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association. | sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ... | 1981 | 7284955 |
| in-vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains. | intra-peritoneal (i.p.) infection of mice with herpes simplex virus type 2 (hsv 2) attracted macrophages into the peritoneum. macrophages from moderately and highly hsv 2 resistant mouse strains expressed elevated phagocytosis activity 24 hours after injection. stimulation of phagocytosis in low resistant strains was generally less effective or absent. this was, in some experiments, due to the fact that macrophages were already highly activated before the experimental infection. i.p. infection a ... | 1981 | 7319542 |
| tumorigenicity of herpesvirus-transformed cells correlates with production of plasminogen activator. | our studies first demonstrated that established hamster cell lines transformed in vitro by herpesviruses activate plasminogen more effectively than normal hamster fibroblasts. this ability is probably due to increased levels of the enzyme plasminogen activator (pa). in the studies described here, the 333-8-9 cell line, originally transformed by herpes simplex virus type 2 strain 333, was used to derive subclonal lines that maintained stable pa phenotypes over the course of long in vitro passage. ... | 1981 | 6100964 |
| herpes simplex virus types 1 and 2 and multiple sclerosis. | a hypothesis is proposed which states that multiple sclerosis (ms) is caused by herpes simplex virus type 2 (hsv-2) in persons lacking herpes simplex virus type 1(hsv-1) immunity. it is examined by comparing the epidemiology of these viruses with that of ms. the age at which hsv-2 infections are first detected, as well as the age of peak incidence are similar to those for ms. if age-specific hsv-1 immunity data from various places is compared, an inverse relationship of immunity levels with lati ... | 1981 | 6116906 |
| correlation of the chemical structure of 4-nitroquinolines inactivating human cytomegalovirus and established in vivo carcinogenicity tests. | inactivation of the infectivity of human cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) has been observed following exposure to 4-nitroquinoline 1-oxide (nqo) or its metabolite, 4-hydroxyaminoquinoline 1-oxide (haqo). the present study of the specificity of the chemical structure of 4-nitroquinolines demonstrated that both the 4-nitro and 1-oxide groups were required for inactivation of virus infectivity. reduction of the 4-nitro group to a 4-hydroxyamino group ... | 1980 | 6119808 |
| inhibition of herpes simplex virus type 2-induced biochemical transformation by interferon. | the effect of interferon on the biochemical transformation of thymidine kinase-deficient cells by uv-inactivated herpes simplex virus type 2 has been studied. transformation was much less sensitive to the action of interferon than virus multiplication. however, the continuous presence of a high dose of interferon (2,000 u) inhibited transformation almost completely. although we could not differentiate between the effect of interferon on fixation and expression of the virus thymidine kinase gene, ... | 1980 | 6154151 |
| biochemical transformation of mouse cells by a purified fragment of marmoset herpesvirus dna. | although the size of marmoset herpesvirus (marhv) dna, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (hsv-1) dna, the restriction endonuclease sites of marhv and hsv-1 dnas were quite different. a specific bamhi restriction fragment (6.2 x 10(6) daltons) of marhv dna biochemically transformed lm(tk-) mouse fibroblasts to the thymidine kinase(tk)-positive phenotype. rabbit antisera, prepared against marhv tk, inhibited marhv-induced t ... | 1980 | 6154671 |
| structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. | capsids of herpes simplex virus (hsv) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed nc-1 through nc-7 in order of descending molecular weight). antibodies prepared to hsv-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against hsv-1-infected kb cells. three of the antibodies (anti-nc-1, anti-nc-2, and anti-nc-3,4) also reacted with hsv-2-infected cells. tryptic peptide anal ... | 1980 | 6154808 |
| glycoproteins with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus types 1 and 2. | agar immunodiffusion tests demonstrated that bhk 21 cells infected with either hsv i or hsv 2 release only a few hsv-specified antigens into the extracellular fluid (infected cell released polypeptides-icrp). neutralization blocking experiments showed that the majority of antigens/(including the band ii common antigen) involved as target sites in antibody-mediated virus neutralization are present in the icrp of both hsv i and hsv 2.sds-page identified six regions of virus-specified proteins in t ... | 1980 | 6156995 |
| analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus. | peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. in contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. the effect was age dependent. macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for ... | 1980 | 7399667 |
| bilateral necrotizing retinitis complicating fatal encephalitis probably due to herpes simplex virus type 2. | the clinical history, autopsy findings and ocular pathology of a case of localized neonatal herpes simplex virus (hsv) type 2 infection is reported in which the main brunt of the infection fell on the cerebral hemispheres and the peripheral retina. hsv type 2 was isolated from skin vesicles which developed on the trunk and arms and in the mouth 10 days after birth. there was no visceral involvement. although the infant was probably infected at some time during birth, hsv could not be isolated fr ... | 1980 | 7454228 |
| topical therapeutic efficacy of 9-(2-hydroxyethoxymethyl)guanine and 5-iodo-5'-amino-2',5'-dideoxyuridine on oral infection with herpes simplex virus in mice. | the therapeutic efficacy of two new antiviral agents, 5-iodo-5'-amino-2', 5'-dideoxyuridine (aidurd) and 9-(2-hydroxyethoxymethyl)guanine (acv), in the model of mouse lip inoculated with herpes simplex virus type 2 is reported. the effects on development of clinical lesions, viral replication in the inoculated lips, and establishment of latent viral infection in the trigeminal ganglia were observed. the earlier the treatment with aidurd and acv was initiated after inoculation, the better was the ... | 1980 | 6966305 |
| relationship of antibody to outcome in neonatal herpes simplex virus infections. | neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ... | 1980 | 7216423 |
| estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method. | a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ... | 1980 | 7219207 |
| new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea and tannic acid ointment]. part ii: female patients. | clinical treatment of 147 female patients suffering with genital herpes simplex virus were conducted in two phases. the purpose of phase i was to note the efficacy of treatment with ultrasound and herpigon ointment (zinc, urea, and tannic acid) in relieving pain and shortening the length of time involved in healing. results indicated that the combined treatment significantly reduced pain and length of healing period. phase ii involved a 2-year follow-up of patients with primary and recurrent inf ... | 1980 | 6931874 |
| anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy. | e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ... | 1980 | 6263181 |
| interaction of herpesvirus with spleen cell subpopulations comparison of a neurotropic and a lymphotropic virus. | we studied the interaction of a neurotropic herpesvirus, herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2), and a lymphotropic herpesvirus, guinea pig herpes-like virus (hlv), with guinea pig spleen cells. both hsv-1 and hsv-2 and hlv can attach to and penetrate into b- or t-enriched cells. less than 1.4% of the total b- or t-enriched cell populations were susceptible to infection by hlv and to some degree to hsv-1 or hsv-2 as determined by infectious center assays. after specific antiserum ... | 1980 | 6262239 |
| effect of corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in balb/c mice. | treatment of 4- to 6-week-old, 18- to 22-g male balb/c mice with 0.6 mg of corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (hsv-2). optimal protection occurred when c. granulosum was injected 1 week before hsv-2 infection. c. granulosum-induced resistance to hsv-2 lasted up to 4.5 weeks. studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated im ... | 1980 | 6262241 |
| depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced tumors. | total hemolytic complement (ch50) levels were compared in sera from normal hamsters and hamsters bearing tumors derived from herpes simplex virus type 2-transformed cells ch50 in normal sera ranged from 160 to 212 while ch50 in tumor bearer sera ranged from 82 to 146. preincubation of tumor bearer sera with cell surface proteins (csp) from homologous herpes simplex virus type 2-derived tumor cells resulted in a 66% depletion of ch50 whereas preincubation with heterologous herpes simplex virus ty ... | 1980 | 6258785 |
| immunity to herpes simplex virus type 2 (hsv-2). i. development of virus-specific lymphoproliferative and leukocyte migration inhibition factor responses in hsv-2-infected guinea pigs. | 1980 | 6258811 | |
| expression of type-common envelope antigens by herpes simplex virus type 2-transformed hamster cells. | at least ten polypeptides were detected on the surface of the herpes simplex virus type 1 (hsv-1) virion. two of these polypeptides, having molecular weights of 30,000 and 33,000, were identified in two herpes simplex virus type 2-transformed cell lines (333-8-9 and 333-2-29) by immunoprecipitation and sds-page. the same two virion polypeptides previously were shown to be present in an hsv-1-transformed cell line (14-012-8-1, t-10). these observations suggest that genetic information coding for ... | 1980 | 6259083 |
| cloning of herpes simplex virus 2 dna fragments in a plasmid vector. | dna isolated from virions of herpes simplex type 2 (hsv-2) strain 333 was digested with various restriction enzymes and joined to the ek2 plasmid vector pbr322. the viral dna sequences present in the hybrids were analyzed by restriction enzyme mapping and hybridization to fragments of hsv-2 dna. the collection of recombinant molecules represents approx. 75% of the hsv-2-genome. in most cases, the structure of the recombinants seemed identical to the organization of authentic fragments of hsv-2 d ... | 1980 | 6260572 |
| experimental studies on genital herpetic infection in mice. | female icr mice were infected with hsv-1 and hsv-2 by inserting a cotton pellet soaked in viral solution (10(7-8) pfu/m1) into the vagina. the appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. these histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. results of fluorescent antibody studies on the appearance of vir ... | 1980 | 6266394 |
| influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine. | the inhibition of herpes simplex virus type 1 (hsv-1) plaque formation by acycloguanosine (acg) was assayed in human fetal lung fibroblasts (hl), cell lines from human cervical carcinoma, rabbit cornea, and human rhabdomyosarcoma, and three green monkey kidney cell lines. the acg concentration giving 50% plaque reduction (pr50) of hsv-1 was lowest in hl. in two green monkey kidney cell lines, hsv-1 plaque formation was relatively insensitive to acg, with pr50 of 25 and 60 mum, respectively. in t ... | 1980 | 6265398 |
| localization of the thymidine kinase gene of herpes simplex virus type 2 (333). | the thymidine kinase (tk) gene of herpes simplex virus type 2 (hsv-2) has been identified on purified restriction endonuclease fragments of hsv-2 dna. these fragments were localized on the physical map of hsv-2 dna. a detailed map of the hsv-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). mouse tk- cells that were biochemically tr ... | 1980 | 6263824 |
| comparison of foldback sequences of herpes simplex virus types 1 and 2 dna. | the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ... | 1980 | 6243346 |
| association of herpes simplex thymidine kinase gene with chromosome no. 18 in transformed human cells. | the herpes simplex virus type-2 (hsv-2)-transformed human cell line hb-2-3 was fused with thymidine kinase (tk)-deficient mouse cells [lm(tk-)], and 12 independent hybrids were isolated with the use of the hat (hypoxanthine, amethopterin, and thymidine)-ouabain selection system. discontinuous polyacrylamide gel electrophoresis studies demonstrated that the hsv-2-specific tk was the selected enzyme in the hybrids. isoenzyme analysis and karyotyping were used in the analysis of the hybrids for the ... | 1980 | 6243715 |
| suppression by progesterone of nonspecific in vitro lymphocyte stimulation in mice as a mechanism for the enhancement of herpes simplex virus type 2 vaginal infection. | the role of antibody, interferon, and cell-mediated immunity (cmi) were studied to determine the mechanisms for progesterone enhancement of vaginal herpes simplex virus type 2 (hsv 2) infection in mice. three groups of mice were studied: nonpregnant control, pregnant, and nonpregnant progesterone-treated mice. vaginal infection with hsv 2 did not elicit a neutralizing antibody or a systemic interferon response in any of the groups tested. splenic lymphocytes from noninfected and infected mice we ... | 1980 | 6243861 |
| treatment of genital herpes simplex virus in male patients. | twenty-three male patients with herpes simplex virus type 2 blisters on the prepuce, glans penis, and penile shaft were divided into 2 groups. ten patients served as controls, and thirteen patients were treated with ultrasound (1 w/cm2 for 60 sec) and herpigon on 3 consecutive days. results showed a negative virus culture after 3 days of treatment and significant reduction of the recurrent infection in the treated group while the control group experienced recurrent infection within 62--80 days. ... | 1980 | 6243914 |
| characterization of herpes simplex virus strains isolated from patients with various diseases. | herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ... | 1980 | 6243918 |
| different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits. | skin infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ... | 1980 | 6244227 |
| distribution of sequences homologous to the dna of herpes simplex virus, types 1 and 2, in the genome of pseudorabies virus. | the distribution of related sequences between the genomes of pseudorabies virus (prv) and herpes simplex virus, types 1 (hsv-1) and 2 (hsv-2), was determined. approximately 7% of the sequences in prv are shared by hsv-1 and hsv-2 dnas. by means of the southern blot technique, it was found that the homologous sequences are not sequestered in one region but are distributed throughout the prv genome. hsv-1 and hsv-2 have the greatest homology with the long unique region of prv dna and the least wit ... | 1980 | 6244240 |
| reduction of cell surface fibronectin (lets protein) correlates with tumorigenicity of hamster fibroblasts transformed by herpes simplex virus type2. | a series of herpes simplex virus type 2 (hsv-2)-transformed hamster cells has been examined using the technique of lactoperoxidase-catalyzed cell surface protein iodination. the amount of cell surface fibronectin (lets protein) was reduced after hsv-2 transformation, and this reduction correlated with increased tumor-forming ability. | 1980 | 6244242 |
| a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein. | a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ... | 1980 | 6244352 |
| analysis of viral dna sequences in hamster cells transformed by herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) dna was treated with four restriction endonucleases (ecori, hinciii, bgl ii, and xba i) and eight fragments were purified and labeled with 32p in vitro. the kinetics of renaturation of each of the fragments was measured in the presence of dna extracted from 333-8-9, a hamster cell line transformed by uv light-inactivated hsv-2 strain 333, and from a series of cloned derivatives and their tumor lines. all of the lines examined contained a partial set of viral s ... | 1980 | 6244590 |