Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| frequency of herpes simplex virus-specific helper t lymphocyte precursors in the lymph node cells of infected mice. | we have established a limited dilution assay to estimate the frequency of herpes simplex virus type 1 (hsv)-specific, interleukin 2 (il 2)-producing helper t lymphocyte precursors (htl-p). the estimated frequency of such cells in suspensions of local lymph node (ln) cells 5 days after in vivo virus infection was 1:2470 to 1:5800. frequencies of htl-p in cells from uninfected mice were below levels of detection of our system and were judged to be below 1:100,000. removal of lyt-2+ cells from resp ... | 1985 | 2579150 |
| antibody response to type-common and type-unique epitopes of herpes simplex virus polypeptides. | herpes simplex virus type 1 (hsv1) and type 2 (hsv2) polypeptides with type-common and type-unique epitopes were identified using cross-adsorbed hyperimmune rabbit sera and western blotting techniques. twelve hsv1 and fourteen hsv2 polypeptides with type-specific epitopes were identified. cross-adsorbed human sera reacted to a subset of the type-specific epitopes defined by rabbit sera. human sera with both hsv1- and hsv2-specific antibodies were identified by their reaction to hsv1-type-specifi ... | 1985 | 2580054 |
| herpes simplex virus latency in a hyperresistant clone of mouse neuroblastoma (cl300) cells. | herpes simplex virus (hsv) hyperresistant neuroblastoma cells (clone cl300 rii) were latently infected with hsv-1 if cultured in presence of hsv-neutralizing antibody for one or two passages after infection and then passaged further without antibody. by superinfecting hsv-1 latently infected cl300 rii cells with hsv-2, progeny virus with hsv-1 characteristics was regularly rescued. such retrieval of hsv-1 decreased with passage of the latently infected cells. | 1985 | 2982359 |
| protection from genital herpes simplex virus type 2 infection by vaccination with cloned type 1 glycoprotein d. | guinea pigs were vaccinated with truncated herpes simplex virus type-1 (hsv-1) glycoprotein d produced in the genetically engineered mammalian cell line gd10.2. vaccinated animals formed antibodies that neutralized both hsv-1 and herpes simplex virus type 2 (hsv-2) in an in vitro neutralization assay. vaccinated animals were challenged with hsv-2 by intravaginal infection. animals that received the immunogen in freund's complete adjuvant were completely protected from the clinical manifestations ... | 1985 | 2983428 |
| anti-herpes simplex virus and cytostatic activity of some new 5-substituted 1-(4-hydroxybutyl)-and 1-(2-hydroxyethoxymethyl) uracil nucleoside analogues. | two series of 5-substituted acyclic uracil nucleoside analogues (5-x-acyclo-u) were evaluated for their inhibitory effects against three herpes simplex virus type 1 (hsv-1) strains and one type 2 (hsv-2) strain in a plaque inhibition assay on human embryonic lung fibroblast (helf) cell cultures as well as for their ability to inhibit the proliferation of baby hamster kidney cells in suspension (bhk-s) culture. acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] and (s)-9-(2,3-dihydroxypropyl)adeni ... | 1985 | 2864819 |
| evidence for a direct role for both the 175,000- and 110,000-molecular-weight immediate-early proteins of herpes simplex virus in the transactivation of delayed-early promoters. | we reconstructed the regulated induction of delayed-early (de) transcription that occurs during herpes simplex virus (hsv) infection by using a transient expression system in which recombinant target genes were cotransfected into vero cells together with intact activating genes. plasmids containing cloned hsv-1 or hsv-2 immediate-early (ie) genes stimulated by up to 100-fold the expression from recombinant constructs containing the bacterial chloramphenicol acetyltransferase (cat) gene under the ... | 1985 | 2983086 |
| antiviral activity of cp-20,961 against herpes simplex viruses in vitro. | the antiherpesvirus activity of cp-20,961 [n,n-dioctadecyl-n',n'-bis (2-hydroxyethyl) propanediamine, or avridine] was investigated in cultured guinea pig embryo (gpe) cells. plaque formation of herpes simplex virus type 1 (hsv1) and type 2 (hsv2) was inhibited, but vesicular stomatitis virus replication was not inhibited, in gpe cells treated with cp-20,961 before infection. the id50 concentration of cp-20,961 for hsv was about 50 micrograms/ml for 3-4 days of pretreatment. after virus adsorpti ... | 1985 | 2982759 |
| herpes simplex virus type 1 infection of endothelial, epithelial, and fibroblast cells induces a receptor for c3b. | we recently demonstrated that herpes simplex virus type 1 (hsv 1) induces a receptor on human umbilical vein endothelial cells for complement component c3b (c3br). we assigned this receptor function to hsv 1 viral glycoprotein c (gc) based on several observations: tunicamycin, which prevents glycosylation and expression of n-linked glycoproteins on the surface of infected cells, markedly reduced expression of the c3br; monoclonal antibodies to hsv 1 gc blocked detection of the c3br, whereas mono ... | 1985 | 2982950 |
| the herpes simplex virus amplicon: analyses of cis-acting replication functions. | previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. the present study established that two separate cis-acting functions--a dna replication origin and a cleavage/packaging signal--are required for amplicon propagation. using deleted derivatives of cloned amplicons, we mapped one of the viral dna replication origins (ori-2 or oril) at coordin ... | 1985 | 2983310 |
| acute and latent infection by thymidine kinase mutants of herpes simplex virus type 2. | 1985 | 2982968 | |
| herpes simplex virus hepatitis in mice: effects of treatment with trisodium phosphonoformate. | clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) induced focal necrotic hepatitis in icr/schö mice. the extent of the hepatitis could be considerably reduced by trisodium phosphonoformate (pfa) administered for 3 days in a daily dose of 400-600 mg/kg. the hsv hepatitis in mice could serve as a suitable model for testing antiherpetic drugs. | 1985 | 2869661 |
| synergistic antiviral effects of acyclovir and vidarabine on herpes simplex infection in newborn mice. | 277 5-7-day-old white swiss mice were infected intranasally with herpes simplex virus type 2 (hsv2) to mimic disseminated herpetic infection in human newborns. the antiviral efficacy of acyclovir, vidarabine and specific antiserum (sas) alone and in combination was assessed. a significant increase in survival rate (52%) was observed with the combination of acyclovir (80 mg/kg/day) and vidarabine (125 mg/kg/day) compared to either compound (24 and 6%, respectively) when therapy was begun 30 h aft ... | 1985 | 4028874 |
| the effect of prostaglandin e2 on the initial immune response to herpes simplex virus infection. | numerous clinical situations, demonstrated to be associated with reactivation of herpes simplex virus infections, have also been shown to produce increases in local levels of prostaglandins. the current study was initiated to determine if prostaglandins play a role in the immune response and control of herpes simplex virus infections. lymphocytes from volunteers were stimulated with concanavalin a, herpes simplex virus 1, or herpes simplex virus 2 in the presence of prostaglandin e2 or ibuprofen ... | 1985 | 3872073 |
| human monocyte-mediated cytotoxicity against herpes simplex virus-infected cells: activation of cytotoxic monocytes by free and liposome-encapsulated lymphokines. | human peripheral blood monocytes were incubated with free or liposome-encapsulated human lymphokines containing macrophage-activating factor (maf) and tested for their effect on herpes simplex virus (hsv)-infected target cells. activated monocytes lysed allogeneic hsv type 2 (hsv-2)-infected whole human embryo cells and xenogeneic balb/c mouse embryo cells (10e2) without any significant effect on uninfected cells, as measured by release of 51cr from target cells after 18 h of cocultivation. kine ... | 1985 | 3882870 |
| viral meningitis. | viral meningitis is part of the aseptic meningitis syndrome but must be distinguished from bacterial meningitis on the basis of a careful examination of the csf and sound clinical judgment. enteroviruses probably account for the bulk of cases of aseptic meningitis that occur in the united states and which are reported to the centers for disease control each year. the seasonal pattern in the incidence of aseptic meningitis is largely due to the seasonal variation of enteroviral infections. early ... | 1985 | 3990441 |
| cigarette smoking, herpes simplex virus type 2 infection, and cervical abnormalities. | in a case-control study, we investigated the relation of cigarette smoking and genital herpes virus infection to cervical abnormalities. although cases and controls differed according to several factors, multiple logistic analyses indicated that age, education, and cigarette smoking were the only independent risk factors for histologically confirmed disease. relative risk estimates were 1.92 and 3.68 for past and current smokers, respectively. the results indicated that smoking and genital herpe ... | 1985 | 4003639 |
| nucleosides. 133. synthesis of 5-alkenyl-1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines and related pyrimidine nucleosides as potential antiviral agents. | the synthesis of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines with a halovinyl or vinyl substituent at c-5 was accomplished from the corresponding 5-iodo (fiac, 1) and/or 5-chloromercuri nucleoside analogues with use of li2pdcl4- and pd(oac)2-mediated coupling reactions. thiation of the benzoylated derivative of the 5-ethyluracil nucleoside 3 followed by s-methylation and then ammonolysis provided 5-ethyl-2'-fluoro-ara-c. 5-ethynyl-2'-fluoro-ara-c (19a) and 5-ethynyl-2'-fluoro-ara-u (19 ... | 1985 | 4009596 |
| degradation of cellular mrnas induced by a virion-associated factor during herpes simplex virus infection of vero cells. | we have used northern blot hybridization to study the accumulation of specific cellular mrnas in vero cells infected with herpes simplex virus (hsv) type 1 or type 2. hsv-1 infection decreased the cytoplasmic levels of beta- and gamma-actin, beta-tubulin, and histone h3 and h4 mrnas, though not all at the same rate. hsv-2 infection resulted in a more rapid decrease in actin and histone mrna levels compared with hsv-1 infection. the turnover rate of each type of mrna studied was accelerated in hs ... | 1985 | 4020960 |
| [herpes simplex virus infections]. | there are two serological types of herpes simplex virus, hsv-1 and hsv-2. the types can be differentiated by different methods. after a primary infection hsv reaches the sensory ganglions by the way of peripheral nervous tracts. the virus remains for life in the ganglion as virus genome. after activation and return movement a new infection can appear. a lot of various diseases are caused by primary infection, reactivation or reinfection. the diagnosis in herpes virus diseases includes the detect ... | 1985 | 4039869 |
| the effect of dimethylnitrosamine on host resistance and immunity. | adult female b6c3f1 mice were injected ip with 0.2 ml phosphate-buffered saline (pbs) only or pbs containing 1.5, 3, or 5 mg dimethylnitrosamine (dmn)/kg body wt daily for 14 days. on day 16, mice were evaluated for changes in immune status as measured by the antibody response to sheep red blood cells (srbcs), blastogenesis to t- and b-cell mitogens, natural killer (nk) cell function, delayed hypersensitivity, and alveolar macrophage (am) bactericidal activity; and for changes in host resistance ... | 1985 | 3883572 |
| [isolation of an endogenous guinea pig herpeslike virus from hartley guinea pigs with latent herpes simplex virus type 2 infection]. | 1985 | 3017012 | |
| [discovery of herpes simplex virus (hsv)-like particles in biopsy samples of cervical carcinoma]. | the tissue of cervical cancer and hsv-2 infected baby rabbit kidney (brk) cells were studied comparatively by electron microscope. in 2 out of 12 samples from patients with cervical carcinoma, some virus-like particles were observed in the cytoplasm or intercellular space. some particles seemed to be enveloped by endoplasmic reticulum. according to their size, structure and morphology, these particles resembled the herpes simplex virus. the relationship between the discovery of herpes simplex vi ... | 1985 | 3015529 |
| selective in vitro and in vivo activities of 5-(2-haloalkyl)pyrimidine nucleoside analogs, particularly 5-(2-chloroethyl)-2'-deoxyuridine, against herpes simplex virus. | 5-(2-chloroethyl)-2'-deoxyuridine (cedu), 5-(3-chloropropyl)-2'-deoxyuridine (cpdu), and 5-(2-chloroethyl)-2'-deoxycytidine (cedc) were evaluated for activity against herpes simplex virus type 1 (hsv-1) and hsv-2 in vitro. their mics for hsv-1 in primary rabbit kidney cell cultures were 0.15, 0.20, and 0.60 micrograms/ml, respectively; their mics for hsv-2 were about 10-fold higher. when tested in parallel, the reference compounds 5-ethyl-2'-deoxyuridine, 5-iodo-2'-deoxyuridine, acyclovir, and ( ... | 1985 | 3010841 |
| retention of herpes simplex virus type ii sequences in bglii n-transformed cells after co-transfection with a selectable marker. | transformation of mammalian cells by total u.v.-inactivated herpes simplex virus ii (hsvii) or cloned fragments thereof (bglii n, bglii c) has been complicated both by a low efficiency of oncogenic transformation and the disappearance of viral dna and/or viral products initially detected in the transformed cell lines. in an attempt to effect a stable integration of bglii n and to elucidate the role of hsvii in oncogenic transformation, we have co-transfected nih 3t3 cells with pag60, a plasmid w ... | 1985 | 3004960 |
| acyclovir prophylaxis in bone marrow transplant recipients. | forty-two patients undergoing bone marrow transplantation were included in a randomised, double-blind and placebo controlled trial of prolonged acyclovir prophylaxis against infections with viruses of the herpes group. twenty patients were allocated to receive acyclovir and 22 to receive placebo. acyclovir or placebo was administered i.v. at a dose of 250 mg/m2 twice daily, starting 5 days before transplantation. at 5 weeks after transplantation, administration was changed to tablets, 400 mg thr ... | 1985 | 3006228 |
| virological aspects on herpesvirus infections. | the family herpesviridae include five important human pathogens, herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv) and cytomegalovirus (cmv). the five viruses are represented in all three herpesvirus subfamilies, a subdivision based on differences in phenotypic properties such as length of replication cycle, cytotoxic properties and host cell range. the herpesviruses have dna genomes sharing characteristic anatomical features. they replicate in the nuclei of eukaryo ... | 1985 | 3006235 |
| [inhibitory effect of tai-ding-an on dna and protein synthesis of herpes simplex virus type 2 (hsv-2)]. | 1985 | 3008965 | |
| [herpes simplex virus type 2 infection. etiology, clinical aspects, diagnosis, therapy, hsv-2 and cervix cancer]. | the genital herpes infection is mostly caused by hsv-2. the analysis of epidemic data supports the common impression that herpes genitalis markedly increased. the hsv-2 is with few exceptions a sexually transmitted virus, the infectionsity is about 80%. the primary infection with hsv-2 occurs in the youth. hsv-2 survives in a latent stage in the human body for life and causes as a rule to recurrent diseases. the clinical picture is variable. the most severe complication is the sepsis of newborn. ... | 1985 | 3004067 |
| [demonstration of latent infection by herpes simplex virus type 2 (hsv-2) in the spinal cord of the guinea pig]. | 1985 | 3008964 | |
| a close association of adriamycin resistance with expression of cell surface antigens in adriamycin-resistant cell lines of herpes simplex virus type 2-transformed syrian hamster cells. | we determined the properties of three adriamycin (adm)-resistant cell lines isolated from a clone of herpes simplex virus (hsv) type 2-transformed syrian hamster cells. the adm-resistant lines (admr-6, -7 and -9) established by continuous exposure of the cells to adm were 20 to 30 times more resistant to adm than was the parent line. these resistant lines exhibited cross-resistance to daunomycin, actinomycin d, chromomycin a3 and vincristine, but not to mitomycin c, bleomycin or methotrexate. up ... | 1985 | 3005209 |
| plaque autoradiography assay for the detection and quantitation of thymidine kinase-deficient and thymidine kinase-altered mutants of herpes simplex virus in clinical isolates. | a plaque autoradiography assay to detect and quantitate thymidine kinase (tk) mutants of herpes simplex virus type 1 (hsv-1) and hsv-2 in clinical samples is described. this method utilizes the selective incorporation of [125i]iododeoxycytidine, a pyrimidine analog selectively phosphorylated by the hsv tk. only cells infected with tk-competent virus will efficiently incorporate iododeoxycytidine and are the only cells detected by autoradiography. furthermore, this assay discriminates between tk+ ... | 1985 | 3010836 |
| cellular proteins expressed in herpes simplex virus transformed cells also accumulate on herpes simplex virus infection. | the cell proteins expressed in rat embryo cells transformed by herpes simplex virus (hsv) have been analysed by immunoprecipitation assays to determine those polypeptides which can be identified by immunoprecipitation with the sera of tumour-bearing animals and also with antisera to herpes simplex infected cells. cell polypeptides commonly recognised by both these sera have been further characterised using a monoclonal antibody directed against a cellular polypeptide which accumulates on hsv-2 l ... | 1985 | 3004943 |
| mechanism of selective inhibition of human cytomegalovirus replication by 1-beta-d-arabinofuranosyl-5-fluorouracil. | four kinds of 1-beta-d-arabinofuranosyl-5-halogenouracil were examined for inhibition of human cytomegalovirus (hcmv) and herpes simplex virus type 1 (hsv-1) and 2 (hsv-2) replication. 1-beta-d-arabinofuranosyl-5-fluorouracil (ara-fu) was the most effective against hcmv, whereas 1-beta-d-arabinofuranosyl-5-bromouracil was the most effective against hsv-1 and hsv-2. the mechanism of action of ara-fu on hcmv replication was also studied. the dttp pool size in human embryonic fibroblasts was increa ... | 1985 | 3010844 |
| vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein d prevents latent herpes in mice. | in humans, herpes simplex virus causes a primary infection and then often a latent ganglionic infection that persists for life. because these latent infections can recur periodically, vaccines are needed that can protect against both primary and latent herpes simplex infections. infectious vaccinia virus recombinants that contain the herpes simplex virus type 1 (hsv-1) glycoprotein d gene under control of defined early or late vaccinia virus promoters were constructed. tissue culture cells infec ... | 1985 | 2986288 |
| synthesis and antiherpetic activity of (s)-, (r)-, and (+/-)-9-[(2,3-dihydroxy-1-propoxy)methyl]guanine, linear isomers of 2'-nor-2'-deoxyguanosine. | racemic 9-[(2,3-dihydroxy-1-propoxy)methyl]guanine [(+/-)-indg], a new analogue of acyclovir (acv) and a structural analogue of 2'-nor-2'-deoxyguanosine (2'ndg), was synthesized and found to inhibit the replication of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2). subsequently, its optical isomers, (r)- and (s)-indg, were prepared from chiral intermediates. the chloromethyl ethers of 1,2-di-o-benzyl-d- and -l-glycerol were made and reacted with tris(trimethylsilyl)guanine to give the 9-alky ... | 1985 | 2989523 |
| crossed immunoelectrophoretic analysis of herpes simplex virus type 2 proteins. characterization of antigen-5. | herpes simplex virus type 2 proteins extracted from infected cells and analysed by crossed immunoelectrophoresis identified a nonglycosylated antigen named ag-5. the antigen contained two proteins when extracted from the agarose gel and the molecular weights were 128k and 91k. both proteins are located in the nucleus of the infected cells and the 128k is identical to icp-8. the 91k protein is based on the reactivity with monoclonal antibodies most likely the alkaline exonuclease mapped by presto ... | 1985 | 2990392 |
| microcarriers in combination with enzyme immunofiltration and immunofluroescence for the detection of herpes simplex virus antigens in culture. | a microcarrier culture system in combination with enzyme immunofiltration with a herpes simplex virus (hsv) group monoclonal antibody was found to be as sensitive as immunofluorescence for the detection of hsv type 1 (hsv-1) in cell cultures and to give specific identification at the same time as the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the appearance of the cytopathic effect with very high infectious inocula and within 10 to 24 h of the ... | 1985 | 2984242 |
| problems associated with the use of (e)-5-(2-bromovinyl)-2'-deoxyuridine for typing herpes simplex virus. | when 0.5 microgram of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) per ml was incorporated directly into cell culture medium inoculated with eight known positive specimens, one herpes simplex virus type 1 (hsv-1) isolate grew in the presence of bvdu and was misidentified. by plaque assay, the titers of 15 hsv-1 strains were reduced by more than 3 log10 by bvdu, and the titers of 16 hsv-2 strains were reduced by less than 2 log10. titers of hsv-1 acyclovir-resistant strains were reduced by less th ... | 1985 | 2984249 |
| [prevention and therapy of herpesvirus infections]. | the group of the human-pathogenic herpesviruses comprises five subgroups: herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), varicella zoster virus (vzv), cytomegalovirus (cmv), and epstein-barr virus (ebv). primary infection with these ubiquitous herpesviruses usually occurs in childhood or during adolescence and frequently remains inapparent. however, it can also give rise to a variety of clinical pictures. important clinical manifestations of herpesvirus infections are ... | 1985 | 2986378 |
| synergism between recombinant human interferon and nucleoside antiviral agents against herpes simplex virus: examination with an automated microtiter plate assay. | an automated, quantitative, cytopathic effect (cpe) inhibition assay with human fibroblasts in 96-well microtiter plates was used to examine the combination of recombinant human interferon-alpha (rifn-alpha a) and acyclovir, vidarabine, or dihydroxypropoxymethyl guanine against herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) in vitro. fifty percent cpe (cpe50) end points, calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, represent ... | 1985 | 2987369 |
| enhanced efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine in combination with gamma interferon against herpes simplex virus type 2 in mice. | the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) and recombinant mouse interferon gamma (rmuifn-gamma) were evaluated for their efficacy alone and in combination against a herpes simplex virus type 2 systemic infection in mice. intraperitoneally infected animals were treated once a day with the drugs at various concentrations for 5 days starting 24 h after inoculation. dhpg was given subcutaneously and rmuifn-gamma intraperitoneally. for dhpg, the effective dose at which 50 ... | 1985 | 2992368 |
| intracellular transport of herpes simplex virus gd occurs more rapidly in uninfected cells than in infected cells. | a mouse l cell line which expresses the herpex simplex virus type 1 immediate-early polypeptides icp4 and icp47 was cotransfected with a cloned copy of the bglii l fragment of herpes simplex virus type 2, which includes the gene for gd, and the plasmid psv2neo, which contains the aminoglycosyl 3'-phosphotransferase (agpt) gene conferring resistance to the antibiotic g418. a g418-resistant transformed cell line was isolated which expressed herpes simplex virus type 2 gd at higher levels than were ... | 1985 | 2987522 |
| identification and typing of herpes simplex virus types 1 and 2 by monoclonal antibodies, sensitivity to the drug (e)-5-(2-bromovinyl)-2'-deoxyuridine, and restriction endonuclease analysis of viral dna. | with development of antiviral drugs, the need to identify a virus as to drug sensitivity becomes increasingly of importance. the compound (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) has been shown to be much more inhibitory to the replication of herpes simplex virus type 1 (hsv-1) and varicella-zoster virus as opposed to herpes simplex virus type 2 (hsv-2). we have typed over 170 isolates, using an immunofluorescent technique and sensitivity to the drug bvdu. these results were then compared to ... | 1985 | 2984324 |
| comparison of western blot analysis to microneutralization for the detection of type-specific herpes simplex virus antibodies. | a newly developed system (as described in the previous paper) employing separated herpes simplex virus (hsv) polypeptides electrophoretically transferred to nitrocellulose paper, western blot analysis (wba), was compared to microneutralization (mn) for the detection of type-specific antibody to hsv types 1 and 2 in 98 human sera. sera containing hsv1-specific antibodies reacted at least twofold more densely (as quantitated by densitometric scanning) to hsv1 than hsv2 polypeptides, while sera wit ... | 1985 | 2984325 |
| synthesis and processing of glycoprotein gg of herpes simplex virus type 2. | monoclonal antibody 13 alpha c5-1-a11 immunoprecipitated two major polypeptides of molecular weights 108,000 and 120,000 from extracts of herpes simplex virus type 2-infected bhk-21 cells labeled with [35s]methionine or [3h]glucosamine. in pulse-chase experiments, both labels were chased from the 120,000-molecular-weight peptide (120k peptide) into the 108k molecule. endoglycosidase h (endo h) reduced the 120k peptide to a 112k peptide but did not affect the 108k peptide. similar profiles were o ... | 1985 | 2987534 |
| synthesis and antitumor and antiviral activities of a series of 1-beta-d-ribofuranosyl-5-halocytosine (5-halocytidine) cyclic 3',5'-monophosphates. | a series of 1-beta-ribofuranosyl-5-halocytosine cyclic 3',5'-monophosphates (1-4) has been prepared. direct halogenation of cytidine 3',5'-monophosphate (ccmp) yielded the cl, br, and i compounds while 5-f-ccmp (1) was obtained on cyclization of the 5'-monophosphate. on in vitro testing of 1-4 against l1210 and p388 leukemias, only 1 showed significant low-level activity (id50 = 3.1 x 10(-4) mmol/l). derivatives 2-4 were inactive at 10(-1) mmol/l and also proved to have low viral ratings against ... | 1985 | 2984417 |
| synthesis and antiviral activity of 11-azapentacyclo[6.2.1.0.0.0]decane. | 11-azapentacyclo[6.2.1.0.0.0]decane (6a) as well as its 6,7-dimethyl derivative 6b was synthesized by a novel, four-step sequence that holds promise for the construction of a variety of cage compounds with bridging nitrogen atoms. the hydrochloride salt of 6a was shown to possess no antiviral activity against either the influenza virus a/victoria/3/75 or the herpes simplex viruses hsv-1 and hsv-2. | 1985 | 2989519 |
| protection of mice against fatal herpes simplex type 2 infection by liposomes containing muramyl tripeptide. | intravenous administration of liposomes containing muramyl tripeptide phosphatidylethanolamine, a lipophilic derivative of muramyl dipeptide that activates macrophages to a cytolytic state in situ, significantly protected mice against lethal challenge with herpes simplex virus type 2. these findings suggest that the systemic activation of macrophages by liposomes containing an immunomodulator can lead to prophylaxis of severe infections caused by herpesviruses. | 1985 | 2984772 |
| oral acyclovir therapy of genital herpes simplex virus type 2 infections in guinea pigs. | oral acyclovir was evaluated for its effectiveness in treating guinea pigs with primary herpes simplex virus type 2 infections. guinea pigs inoculated intravaginally with acyclovir-susceptible strains (for which 50% inhibitory concentrations of acyclovir in cell culture were found to be in the range of 0.15 to 1.2 micrograms/ml) and treated with 5.0 mg of acyclovir per ml in the drinking water beginning 48 h postinfection showed significant reductions in lesion severity. this dosage produced ser ... | 1985 | 2984984 |
| prevalence of serum antibodies to herpes simplex virus types 1 and 2: application of an elisa technique to 100 cases of anogenital herpes. | sera of 100 patients affected by anogenital herpes were tested by an enzyme-linked immunosorbent assay (elisa) technique using partially purified antigens obtained by extraction from the nuclei of infected vero cells. herpes simplex virus type 1 (hsv-1) was isolated from the anogenital lesions of 17 patients and herpes simplex virus type 2 (hsv-2) from 83 patients. the relative proportions of antibody of the igg class to hsv-1 and hsv-2 were determined and their ratio (r) was calculated, except ... | 1985 | 2988144 |
| immunologic characterization of herpes simplex virus type 2 antigens icp10 and icsp11/12. | infected cell protein 10 (icp10) or antigen 4 (ag4) and infected cell-specific protein 11/12 (icsp11/12) have been suggested as specific antigenic markers for cervical carcinoma. experiments were designed to determine whether icp10 and icsp11/12 are distinct antigens and to determine the cellular localization of icp10. results indicate that an apparent 160 kdalton (kda) protein analyzed by 8.5% polyacrylamide gels (= 144 kda protein analyzed by 7.0% polyacrylamide gels) was detected in hsv-2-inf ... | 1985 | 2988230 |
| development of molecular probes for simian herpesvirus detection. | a number of serologic procedures are available for specifically determining past infection due to h. hominis, or h. simiae (b virus). a herpesvirus isolated from primate tissues, however, does not lend itself to specific identification. procedures currently in routine use simply do not provide an unequivocal identification of the isolate. we have previously demonstrated that h. simiae contain polypeptide and glycoprotein antigens distinct from those of other herpesviruses. at selected intervals, ... | 1985 | 2989049 |
| isolation and characterization of deletion mutants of herpes simplex virus type 2 (strain hg52). | we provide evidence that: (i) two variants lacking the xbai site at map coordinate 0.7 have been selected following xbai treatment of the dna of herpes simplex virus type 2 strain hg52; (ii) one of these mutants had lost the 0.7 restriction site due to a deletion of approximately 150 base pairs and in the other the site loss was due to a similar sized sequence insertion; (iii) following xbai treatment, four variants with deletions ranging in size from 1.5 kb (in both trl and irl) to 9 kb in irl ... | 1985 | 2989417 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with trifluorothymidine, phosphonoformate and acycloguanosine against herpes simplex viruses. | methoxymethyldeoxyuridine (mmudr) when used in combination with either trifluorothymidine (f3tdr) or phosphonoformate (pfa) showed synergistic activity against herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) in vitro, whereas mmudr and acycloguanosine (acg) combination was antagonistic against herpes viruses. hsv-1 mutants resistant to acg, arabinofuranosyladenine (ara-a), mmudr or pfa were isolated. drug-resistant hsv-1 virus mutants were analyzed for cross sensitivity to acg, ara-a, f ... | 1985 | 2984988 |
| enzyme-linked immunosorbent assay for determination of antibodies against herpes simplex virus types 1 and 2 in human sera. | a rapid and reproducible enzyme-linked immunosorbent assay (elisa) is described for determining antibodies in human sera against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the sera were absorbed for 30 min with heterologous virus-infected-cell extracts to remove cross-reacting antibodies and then were applied to elisa plates containing the target antigens, immunoaffinity-purified hsv-1 glycoproteins gc and gd and hsv-2 glycoproteins gd and gf. the absorbance index, defined as the ra ... | 1985 | 2985643 |
| evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus. | an indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (hsv). the indirect immunofluorescence assay (electro-nucleonics, inc.) correctly identified 16 hsv type 2 (hsv-2) isolates, but failed to identify 4 of 14 hsv-1 isolates because of background fluorescence and instability of reagents. forty-nine hsv-1 isolates were correctly typed by direct immunofluorescence assay (kallestad laboratories, inc.), but 1 of ... | 1985 | 2985651 |
| direct inactivation of viruses by mcp-1 and mcp-2, natural peptide antibiotics from rabbit leukocytes. | six homologous peptides were purified to homogeneity from rabbit granulocytes or alveolar macrophages and tested for their ability to inactivate herpes simplex virus type 1 (hsv-1). two of the peptides, mcp-1 and mcp-2, showed considerable in vitro neutralizing activity, whereas four structurally homologous peptides (np-3a, np-3b, np-4, and np-5) were relatively ineffective. inactivation of hsv-1 by mcp-1 or mcp-2 depended on peptide concentration and on the time, temperature, and ph of the incu ... | 1985 | 2985808 |
| analysis of world-wide age-specific data for the association of cervical cancer with infections by herpes simplex virus type 2. | world-wide incidence data obtained from the scientific literature are used to expand to age-specific incidence rates the observation that the incidence of invasive cervical cancer in many human populations is related to the incidence of infections with herpes simplex virus type 2 (hsv-2) in the same community. the data for the incidence of the tumor were obtained from international registries. data for prevalence of hsv-2 antibodies were used to evaluate the incidence of past primary infections ... | 1985 | 2989664 |
| the sources of thymidine nucleotides for virus dna synthesis in herpes simplex virus type 2-infected cells. | the thymidine nucleotide sources present during herpes simplex virus type 2 (hsv-2) infection were examined. it was concluded that the source of dttp in hsv-2-infected cells is not only derived from the ribonucleotide reductase-catalyzed de novo pathway, but also from host dna. when the de novo pathway was inhibited by the addition of hydroxyurea, an inhibitor of ribonucleotide reductase, the dttp levels were maintained by a compensatory increase in dttp derived from host dna. the utilization of ... | 1985 | 2997161 |
| incidences of antibodies to hepatitis b, herpes simplex and cytomegalovirus in prostitutes. | the 170 prostitutes registered by the local public health authority (165 female, 5 male; ages 17-61) were serologically investigated by elisa and cft for the presence of hepatitis b virus (hbv) markers (n = 170), anti-cmv (n = 118) and anti-hsv 2 antibodies (n = 117). 4.7% (1.2%) were found to be hbs- (hbe-)antigen carriers; 1.2% revealed an acute hepatitis b (seroconversion of anti-hbc or igm-anti-hbc highly positive); 17.1% presented a past and resolved hepatitis b (anti-hbs positive). these r ... | 1985 | 2990124 |
| antiherpes effects and pharmacokinetic properties of 9-(4-hydroxybutyl) guanine and the (r) and (s) enantiomers of 9-(3,4-dihydroxybutyl)guanine. | three acyclic guanosine analogs with similar structures, the (r) and (s) forms of 9-(3,4-dihydroxybutyl)guanine and 9-(4-hydroxybutyl)guanine, were compared for antiherpes activity in vivo and in vitro. the three guanosine analogs were viral thymidine kinase-dependent inhibitors of virus multiplication. in cell cultures, (s)-9-(3,4-dihydroxybutyl)guanine was the least active of these three drugs against a variety of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) strains. this was also th ... | 1985 | 2990325 |
| analysis of the hsv-2 early ag-4 antigen. | genital herpes simplex virus type 2 (hsv-2) infections can be distinguished from present or past hsv-1 infections by an ag-4 antigen complement fixation assay. the assay which utilizes a 4 hour hsv-2 infected cell extract prepared at a multiplicity of infection (moi) of 1.0 pfu/cell, appears to consist of several viral proteins. studies using monoclonal antibodies, polyclonal rabbit hyperimmune serum, hsv-1 x hsv-2 intertypic recombinant viruses and polyacrylamide gel electrophoresis suggest tha ... | 1985 | 2990385 |
| the effect of triterpenoid compounds on uninfected and herpes simplex virus-infected cells in culture. i. effect on cell growth, virus particles and virus replication. | the related triterpenoid compounds carbenoxolone sodium (cbx) and cicloxolone sodium (ccx) have been investigated in clinical trials for treatment of herpes simplex virus (hsv) infections. when the drugs were tested in vitro, two dose-related effects on bhk cells became apparent: the rate of cell growth was reduced and the drugs exhibited cytotoxicity at high concentrations. flow 2002 cells, in contrast, were apparently unaffected by all drug concentrations tested. the effect of up to 3 days inc ... | 1985 | 2991440 |
| thymidine kinase-deficient herpes simplex virus type 2 genital infection in guinea pigs. | in guinea pigs, thymidine kinase-producing strains of herpes simplex virus type 2 replicated to high titer in the vagina and spinal cord, and animals developed severe clinical disease. infection with thymidine kinase-deficient virus resulted in similar vaginal virus titers; however, animals exhibited little or no clinical illness and only low titers of virus were detected in spinal cord homogenate cultures. neural and extraneural latent infection as well as recurrent infection were noted in anim ... | 1985 | 2991558 |
| drug-resistant mutants of herpes simplex virus type 2 with a mutator or antimutator phenotype. | 1985 | 2991710 | |
| metabolism of the carbocyclic analogue of (e)-5-(2-iodovinyl)-2'-deoxyuridine in herpes simplex virus-infected cells. incorporation of c-ivdu into dna. | the carbocyclic analogues of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and (e)-5-(2-iodovinyl)-2'-deoxyuridine (ivdu), in which the sugar moiety is replaced by a cyclopentane ring and which have been designated as c-bvdu and c-ivdu, respectively, are, like their parent compounds bvdu and ivdu, potent and selective inhibitors of herpes simplex virus type 1 (hsv-1) and, to a lesser extent, herpes simplex virus type 2 (hsv-2) replication. we have now synthesized the radiolabeled c-ivdu analogue, ... | 1985 | 2993282 |
| serum antibodies to the major hsv-2-specified dna-binding protein in patients with an acute hsv infection or cervical neoplasia. | the major hsv-2-specified dna-binding protein (icsp 11/12) was purified from hsv-2-infected cells. elisa and immunoblotting techniques were used to study its antigenicity in hsv-infected patients and patients with cervical neoplasia and control women. patients with an acute hsv-2 infection had clearcut antibody responses to the purified icsp 11/12 preparation. determination of the icsp 11/12 antibodies by elisa revealed considerably higher serum antibody levels in patients with cervical carcinom ... | 1985 | 2993495 |
| synthesis and antiherpes simplex virus activity of 9-[(1,3-dihydroxy-2-propylthio)methyl]guanine. | the synthesis of the thio analogue (thio-dhpg, 2) of 9-[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, 1) is described. the synthesis of 2 proceeded via the condensation of acetoxymethyl sulfide 9 with diacetylguanine 10 to give the protected nucleoside analogue 11. although catalytic hydrogenolysis failed, the benzyl ether functionalities of 11 were successfully cleaved by an acetolysis reaction to furnish 14. ammonolysis of 14 gave 2, which was also transformed to sulfoxide 15 and sulfone 16. ... | 1985 | 2993615 |
| virion component of herpes simplex virus type 1 kos interferes with early shutoff of host protein synthesis induced by herpes simplex virus type 2 186. | herpes simplex virus (hsv) strains hsv type 1 (hsv-1) kos and hsv-2 186 are representative of delayed and early shutoff strains, respectively, with regard to their ability to inhibit protein synthesis in friend erythroleukemia cells. when these cells were simultaneously infected with hsv-1 kos and hsv-2 186, hsv-1 kos interfered with the rapid suppression of globin synthesis induced by hsv-2 186. the observed interference was competitive and not due to exclusion of hsv-2 by hsv-1 at the level of ... | 1985 | 2993660 |
| cytomegalovirus and herpes simplex virus ascending myelitis in a patient with acquired immune deficiency syndrome. | progressive ascending myelitis was the presenting feature of the acquired immune deficiency syndrome (aids) in a homosexual man who also had kaposi's sarcoma, pneumocystis pneumonia, and disseminated cytomegalovirus (cmv) infection. neuropathological studies showed profuse cytomegalic cells throughout the brain and spinal cord, but no inflammatory response. at postmortem examination, cmv and herpes simplex virus, type 2 (hsv-2), were recovered from multiple sites throughout the central nervous s ... | 1985 | 2994553 |
| immunoblotting and enzyme-linked immunosorbent assay analysis of serological responses in patients infected with herpes simplex virus types 1 and 2. | serological responses to soluble membrane and cytoplasmic antigens specified by herpes simplex type 1 (hsv1) and 2 (hsv2) were studied by immunoblotting and by enzyme-linked immunosorbent assay (elisa). in the immunoblotting test, polypeptides migrating like the hsv1-specified glycoprotein c showed type-specific reactivity but could not always be detected. the immunoblotting and elisa results were in agreement when antibody responses to hsv1- and hsv2-specified antigens were compared, and they a ... | 1985 | 2995271 |
| differences in humoral immunogenicity between herpes simplex virus types 1 and 2. | infection of nmri mice with increasing doses of six different strains of herpes simplex virus type 1 (hsv-1) induced increasing levels of neutralizing antibodies. in contrast, three strains of hsv-2, irrespective of the dose, induced only marginal antibody responses. only strain hg 52 (hsv-2) at high doses of infection stimulated antibody formation. the virus content of some organs in 6- to 8-week-old mice appeared to be lower after hsv-2 than after hsv-1 infection. application of immune-modulat ... | 1985 | 2995556 |
| inhibition of herpes simplex virus replication in vitro by human cytotoxic t cell clones and natural killer cell clones. | the abilities of human cytotoxic t cell (ctl) clones and natural killer (nk) cell clones to inhibit the replication of herpes simplex virus (hsv) in vitro were shown. the specificities of clones inhibiting hsv replication were the same as those of cytotoxicity in hsv type specificity and hla restriction, i.e. hsv type 1 (hsv-1) and hsv type 2 (hsv-2) common ctl clones inhibited the replication of both hsv-1 and hsv-2 in autologous cells, but not in allogeneic cells. hsv-1-specific ctl clones inh ... | 1985 | 2995557 |
| acute and latent herpes simplex virus neurological disease in mice immunized with purified virus-specific glycoproteins gb or gd. | groups of 5-week-old balb/c mice were immunized intraperitoneally with approximately 10 micrograms of purified alum-precipitated glycoprotein gb or gd of either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) origin. control mice received injections of alum-precipitated 1% bovine serum albumin (bsa). following a second immunization 4 weeks later, seroconversion was confirmed by demonstrating the presence of glycoprotein-specific antibody by immune precipitation. all animals were challenged ... | 1985 | 2995573 |
| (+/-)-2-amino-3,4-dihydro-7-[2,3-dihydroxy-4-(hydroxymethyl)-1- cyclopentyl]-7h-pyrrolo[2,3-d]pyrimidin-4-ones: new carbocyclic analogues of 7-deazaguanosine with antiviral activity. | 5-allyl-2-amino-4,6-dihydroxypyrimidine (3) was chlorinated and ozonized to yield (2-amino-4,6-dichloro-pyrimidin-5-yl)acetaldehyde (5). acetalization of 5 with ethanol afforded a new pyrimidine intermediate 6 which can lead to 2-amino-3,4-dihydro-7-alkyl-7h-pyrrolo[2,3-d]pyrimidin-4-ones and therefore to carbocyclic analogues of 7-deazaguanosine. the 7-substituent was a cyclopentyl analogue of the arabinofuranosyl moiety in 10a, lyxofuranosyl moiety in 10b, and ribofuranosyl moiety in 10c. comp ... | 1985 | 2995667 |
| effect of dimethyl sulfoxide on interaction of human cytomegalovirus with host cell: conversion of a nonproductive state of cell to a productive state for virus replication. | the effect of dimethyl sulfoxide (dmso) on the interaction of human cytomegalovirus (hcmv) with host cell was studied. confluent state of a human rhabdomyosarcoma cell line (a204) showed a much lower susceptibility to hcmv infection when compared to that in subconfluent actively growing cell cultures. treatment of confluent cultures with dmso, however, converted many nonproductive cells in these cultures to a productive state for virus replication. infectious center assay revealed that approxima ... | 1985 | 2996216 |
| immunoperoxidase staining for the detection of herpes simplex virus antigen in cervicovaginal smears. | destained cervicovaginal smears from eight patients with herpes simplex virus (hsv) infections were stained by means of the peroxidase-antiperoxidase (pap) technique to demonstrate the presence of the hsv type 2 (hsv-2) antigen. positive results were obtained in six of the eight cases, with intense staining for the hsv-2-specific antigen throughout the cytoplasm and nuclei of cells having a ground-glass nuclear appearance as well as in multinucleated giant cells. virus isolation was successfully ... | 1985 | 2996270 |
| herpes simplex viral infection in human neonates: an immunohistochemical and electron microscopic study. | specimens obtained at autopsy from six neonates with herpes simplex virus (hsv) infections were examined microscopically, electron microscopically, and immunohistochemically. coagulative necrosis with inclusions was found in the livers and adrenal glands in all cases, as well as in various other organs, including the spleen, bone marrow, lungs, esophagus, tongue, and thymus, in some cases. distinct hemorrhagic diathesis was found in three cases. no characteristic clinical findings, such as skin ... | 1985 | 2997017 |
| retrieval of latent herpes simplex virus type 1 genetic information from murine trigeminal ganglia by superinfection with heterotypic virus in vivo. | mice previously latently infected with the f strain of herpes simplex virus type 1 (hsv-1) can be successfully colonized with a second virus strain if hsv-2 is introduced at the same peripheral site as hsv-1. on the other hand, hsv-1 strains seemed able mutually to exclude establishment of latency with each other. mice (3 months or 3 years after nasal infection) latently infected with hsv-1 were thus superinfected with hsv-2. the mice were sacrificed 2 days post-infection when hsv-2 replication ... | 1985 | 2991439 |
| the therapeutic efficacy of a xanthate compound on herpes simplex virus in skin lesions of mice and guinea-pigs. | xanthates have recently been shown to inhibit the replication of both dna and rna viruses in vitro. the antiviral activity was exerted only under acidic ph conditions. curative effects in vivo on herpes simplex virus (hsv)-induced skin lesions were only observed when the xanthate compound was administered in the form of an ointment containing acidic buffer (sodium phosphate ph 5.0). advanced hsv-2-induced skin lesions in mice were healed by topical treatment with the xanthate compound. hsv-1-ind ... | 1985 | 2993486 |
| identification of cross-reacting glycoproteins of four herpesviruses by western blotting. | monospecific rabbit antisera against purified herpes simplex virus type 1 (hsv-1) gb, gc or gd antigens or polyvalent rabbit antiserum against equine herpesvirus type 1 (ehv-1)-infected cells was used in western blotting to identify antigenically related proteins in cells infected with hsv-1, hsv-2, bovine mammillitis virus or ehv-1. monomeric and oligomeric polypeptides related to hsv-1 gb were found in cells infected with each of the four herpesviruses. the gc antiserum was specific for hsv-1 ... | 1985 | 2993488 |
| lack of oral hsv-2 in a college student population. | a population of individuals with a high incidence of genital herpes simplex virus type 1 (hsv-1), due most likely to oro-genital contact, was examined to determine the incidence of oral herpes simplex virus type 2 (hsv-2) infection. herpes simplex virus was isolated from the oral cavity of 43 college students whose symptoms ranged from singular lesions of the lips with minimal discomfort to severe oral disease with systemic involvement resulting in lymphadenopathy, chills, sweat, myalgia, and fe ... | 1985 | 2993498 |
| application of antibody to synthetic peptides for characterization of the intact and truncated alpha 22 protein specified by herpes simplex virus 1 and the r325 alpha 22- deletion mutant. | the alpha 22 protein is one of five proteins synthesized immediately after infection of permissive cells with herpes simplex virus 1 and 2 (hsv-1 and hsv-2). on the basis of the reported nucleotide sequence of the hsv-1 gene, we synthesized two peptides containing the predicted amino acids 12 through 23 (12 residues) and 21 through 36 (16 residues) in two hydrophilic domains near the n terminus of the protein. rabbit antisera made against these peptides were then used to characterize the alpha 2 ... | 1985 | 2993651 |
| humoral immune response to herpes simplex virus type 2 glycoproteins in patients receiving a glycoprotein subunit vaccine. | serial serum specimens from 22 herpes simplex virus (hsv)-seronegative recipients of an hsv type 2 (hsv-2) glycoprotein subunit vaccine were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis for the development of antibodies to hsv-2 gb, gd, and g80, a complex of gc and ge. volunteers received 50 (n = 12) or 100 micrograms (n = 10) of vaccine at days 0, 28, and 140; sera were drawn weekly for 8 weeks and again at days 140, 147, and 365. among seronegative volunteers, an ... | 1985 | 2997470 |
| evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens. | an indirect immunofluorescent antibody procedure (ifa) for the detection and typing of herpes simplex virus (hsv) and an enzyme-linked immunosorbent assay (elisa) procedure were compared with conventional viral culture. specimens for culture and elisa were inoculated into serum free viral transport medium (vtm) and, for ifa, onto slides provided in the kit. tissue cultures (mrc-5 and primary rabbit kidney) were inoculated and examined daily for cytopathogenic effect (cpe). the remaining vtm was ... | 1985 | 2998264 |
| a modular system for the assay of transcription regulatory signals: the sequence taatgarat is required for herpes simplex virus immediate early gene activation. | a modular system for assaying the activity of transcriptional regulatory signals based on herpes simplex virus (hsv) promoter and terminator sequences linked to the bacterial chloramphenicol acetyltransferase (cat) gene has been used to study activation of hsv immediate early (ie) gene expression. insertion of the sv40 72 base pair (bp) repeat increased mrna levels by 15-fold thus demonstrating the ability of the hsv ie promoter to respond to a heterologous enhancer. a fragment containing part o ... | 1985 | 2999706 |
| the cervical tumor-associated antigen (icp-10/ag-4) is encoded by the transforming region of the genome of herpes simplex virus type 2. | bglii fragment c mapping between 0.416 and 0.580 map units (mu) on the herpes simplex virus type 2 (hsv-2) genome was used for in vitro translation to identify proteins encoded on this fragment. rna homologous to the bglii c fragment directs the synthesis of three proteins with approximate molecular weights of 144,000, 52,000 and 27,000. the 27,000 dalton protein is encoded by sequences within the ecori/hindiii ae fragment (0.419-0.525 mu) that overlap the immortalizing sequences within bglii c. ... | 1985 | 3001010 |
| herpes simplex virus antigen detection in human acute encephalitis: an immunohistochemical study using avidin-biotin-peroxidase complex method. | autopsy specimens from six patients with clinically diagnosed herpes simplex virus (hsv) encephalitis were studied. since immunocytochemistry has been reported to be a more reliable and successful method to identify hsv as the etiologic agent, antitype 1 hsv (hsv1) and antitype 2 hsv (hsv2) and the avidin-biotin-peroxidase complex (abc) method were applied to brain paraffin sections. positive immunostaining was observed in front-orbital, mediobasal temporal lobes, cingulate gyrus, and insula. th ... | 1985 | 3002087 |
| antiviral activity of 5-ethyl-2'-deoxyuridine against herpes simplex viruses in cell culture, mice, and guinea pigs. | the susceptibility of 3 laboratory strains and 24 clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 5-ethyl-2'-deoxyuridine was determined in plaque reduction assays in vero cells. the median effective doses were 8.6 and 7.8 microm, respectively. the drug was less potent than acyclovir and other related antiviral drugs, but it had a high therapeutic index against both hsv-1 and hsv-2. drug-resistant viruses were readily produced in cell culture. these variants were c ... | 1985 | 3000291 |
| recurrent genital herpes simplex virus infection in guinea pigs. | after recovery from initial genital herpes simplex virus (hsv) infections, female guinea pigs developed spontaneous recurrent infections characterized by discrete erythematous or vesicular herpetic lesions on the external genital skin. hsv type 2 (hsv2) caused significantly more recurrent infections in guinea pigs than did hsv type 1 (hsv1). hsv2-infected animals demonstrated a significant decline in frequency of recurrences over time. the viral nature of the recurrent lesions was confirmed by r ... | 1985 | 3000982 |
| risk of recurrence after treatment of first-episode genital herpes with intravenous acyclovir. | to determine whether intravenous acyclovir treatment for a first episode of genital herpes could prevent or reduce subsequent recurrences, we combined and analyzed the results of two independently conducted, randomized, double-blind, placebo-controlled studies. sixty-one patients were enrolled in the two trials; 30 received the drug, and 31 received placebo. at entry the demographic, epidemiologic, and clinical features of acyclovir- and placebo-treated patients from the two centers showed no si ... | 1985 | 3001952 |
| investigation of the virulence genes of herpes simplex virus 2 by experimental infection in vivo with defined intertypic recombinants of a virulent hsv-2 x an avirulent hsv-1. | for determination of the gene loci for virulence and tropism of herpes simplex virus 2 (hsv-2) intertypic recombinants of hsv-1 strain hfem and hsv-2 strain 3345 were screened for their pathogenicity in the tree shrew. hsv-1 strain hfem is not pathogenic in the tree shrew and can be recovered as a latent virus only from the spleen of the animals; but hsv-2 strain 3345, the other parental virus of the recombinants, was found to be virulent for the tree shrew and colonized the ganglia of latently ... | 1985 | 3003551 |
| [inhibition of virus multiplication by immunoactive peptides]. | in the present study we show that peritoneal macrophages obtained from the mice treated with the immunoactive peptides inhibit the multiplication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), but not that of vesicular stomatitis virus (vsv), and that the intraperitoneal administration of the peptides suppresses the infection with hsv-1 in mice. | 1985 | 3002570 |
| multistep transformation by defined fragments of herpes simplex virus type 2 dna: oncogenic region and its gene product. | diploid syrian hamster embryo cells transfected with bgl ii c fragment of herpes simplex virus type 2 dna acquired a neoplastic phenotype. cultures transfected with its left-hand 64% subclone ecori/hindiii fragment ae (0.419-0.525 map unit) grew into established but nontumorigenic lines. transfection of ecori/hindiii ae-immortalized cells with a 4.4-kilobase sac i/bamhi subfragment within bamhi e (0.554-0.584 map unit; overlaps the right-hand 16% of bgl ii c) converted them to tumorigenicity. th ... | 1985 | 3001705 |
| typing of herpes simplex virus isolates with monoclonal antibodies and by nucleic acid spot hybridization. | fifty-one clinical isolates of herpes simplex virus (hsv) were typed by an enzyme immunoassay (eia) using mouse monoclonal antibodies, by dna spot hybridization, and by restriction enzyme analysis using restriction endonuclease eco ri. extracts of vero cells infected with the isolates were used for coating microtitre plates or denatured and spotted onto nitrocellulose filters. viral antigens passively adsorbed to microtitre plates were detected by an indirect eia using mouse monoclonal antibodie ... | 1985 | 3001118 |
| detection of herpes simplex virus type 2-specific antibody with glycoprotein g. | a recently described herpes simplex virus (hsv) type 2 (hsv-2)-specific glycoprotein (gg-2) was purified on an immunoaffinity column prepared with monoclonal antibody. this purified antigen was used in an immunodot enzymatic assay on nitrocellulose paper for the detection of hsv-2 antibodies in human serum. the test was very sensitive in that hsv-2 antibodies were detected in the convalescent sera of 132 of 134 patients with recurrent genital infections in which hsv-2 had been isolated earlier. ... | 1985 | 3001136 |
| synergistic effect of human leukocyte interferon and nonoxynol 9 against herpes simplex virus type 2. | the nonionic surfactant nonoxynol 9 (np9), in combination with human alpha interferon, synergistically reduced the titer of herpes simplex virus type 2 (hsv-2) in vitro. the degree of synergy was highest at an interferon concentration of 10(3) iu/ml and an np9 dilution of 1:1,500. we postulate that np9 inactivates extracellular hsv-2, whereas interferon inhibits hsv-2 replication at the intracellular level. | 1985 | 3000288 |
| construction of rat cell lines that contain potential morphologically transforming regions of the herpes simplex virus type 2 genome. | hybrid recombinant plasmids were constructed; they were composed of the herpes simplex virus type 2 (hsv2) thymidine kinase (tk) gene and dna sequences of hsv2 that have been reported to induce morphological and/or oncogenic transformation of rodent cells in culture. several plasmids were made in two versions, with or without the simian virus 40 enhancer sequences. these plasmids were employed to transfect contact-inhibited rat-2 tk- cells. it was demonstrated that cell lines with stably integra ... | 1985 | 3000980 |
| herpes simplex virus type 2 and human papillomavirus type 16 in cervicitis, dysplasia and invasive cervical carcinoma. | sera and biopsies of cervical lesions from 55 women with benign or malignant disease were analyzed for evidence of infection with herpes simplex virus type 2 (hsv-2) or human papillomavirus (hpv). in addition, information regarding known risk factors for cervical cancer was obtained by interview. the sera were tested for hsv-2 antibodies and the biopsies were tested for hpv or hsv dna sequences by southern blot hybridization. hsv-2 sequences were detected in 2/13 (15%) invasive neoplasms and in ... | 1985 | 2981783 |
| the consensus sequence ygtgttyy located downstream from the aataaa signal is required for efficient formation of mrna 3' termini. | our previous dna sequence comparisons of 3' terminal portions from equivalent herpes simplex virus type 1 (hsv-1) and hsv-2 genes identified a conserved sequence (consensus ygtgttyy; y = pyrimidine) located approximately 30bp downstream from the aataaa signal. we report here that this signal is located downstream from 67% of the mammalian mrna 3' termini examined. using constructions with the bacterial chloramphenicol acetyl transferase (cat) gene linked to an hsv 'terminator' fragment, we show ... | 1985 | 2987822 |