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parameters distinguishing herpes simplex virus type 2-transformed tumorigenic and nontumorigenic rat cells. 19816256070
herpesviruses and parkinsonism. herpes simplex virus types 1 and 2, and cytomegalovirus antibodies in serum and csf.antibodies against herpes simplex virus (hsv) types 1 and 2 and cytomegalovirus (cmv) were assayed with a microindirect hemagglutination (iha) test in the serum of 67 pairs of patients with parkinson's disease and controls. cerebrospinal fluid from 30 pairs was assayed. all patient and control serum was tested with a radioimmunoassay (ria) for antibodies against hsv type 1 subunit antigens. serum iha antibody level against hsv type 1 was increased in patients with parkinson's disease and ria ant ...19816257211
synthesis and antiviral properties of (z)-5-(2-bromovinyl)-2'-deoxyuridine.(z)-5-(2-bromovinyl)uracil was obtained by photoisomerization of the e. isomer. similarly, (e)-5-(2-bromovinyl)-2'-deoxyuridine gave the required z isomer. (z)-5-(2-bromovinyl)-2'-deoxyuridine is much less active against herpes simplex virus type 1 (hsv-1) and somewhat less active against herpes simplex virus type 2 than is the e isomer. both isomers show similar activity against vaccinia virus. therefore, the highly potent and selective activity of (e)-5-(2-bromovinyl)-2'-deoxyuridine against h ...19816265638
differences in the morphology of herpes simplex virus infected cells. ii. type specific membrane alterations of hsv-1 and hsv-2 infected cells.the two types of herpes simplex virus (hsv-1, hsv-2) induced significantly different alterations in the morphology and permeability of infected cells. hep-2 cells infected with hsv-1 (strain thea) were characterized by the formation of polynuclear syncytia. in contrast, after infection with hsv-2 (strain d316, dd), the cells were rounded up. the hsv-1 strains kos and ls5039 and the hsv-2 strain 196 induced both types of cytopathic effect. as shown by comparative scanning and transmission electro ...19816265748
herpesvirus-induced antigens in squamous-cell carcinoma in situ of the vulva.antigens induced by herpes simplex virus type 2 (hsv2) were found to be associated with squamous-cell carcinoma in situ of the vulva in nine of 10 patients. the hsv2-induced antigens are dna-binding proteins that are normally present in the nuclei of infected cells, but in the cells of the carcinomas in situ they were found in the cytoplasm. whole-virion structural antigens were not present, although there was serologic evidence of previous hsv2 infection in patients tested for the presence of a ...19816265780
specific phosphorylation of e-5-(2-iodovinyl)-2'-deoxyuridine by herpes simplex virus-infected cells.e-5-(2-iodovinyl)-2'-deoxyuridine (ivdurd), a highly selective and potent anti-herpes agent, is phosphorylated by primary rabbit kidney (prk) cells that have been infected with either herpes simplex virus type 1 (kos) or herpes simplex virus type 2 (g). however, ivdurd is not phosphorylated by mock-infected prk cells or prk cells that have been infected with a thymidine kinase-deficient mutant of herpes simplex virus type 1 (b 2006). these observations strengthen the concept that the selectivity ...19816263899
mechanisms of expression of herpes simplex virus-common surface antigens in clonal cells of a herpes simplex virus type 2-transformed line.rabbit antiserum hyperimmune to herpes simplex virus type 1 was used to study the expression of herpes simplex virus type-common surface antigens (csa) by indirect immunofluorescence tests in three representative cell clones isolated from a herpes simplex virus type 2-transformed hamster line, 155-4. these three clones showed different phenotypes with respect to csa expression: (i) a csa-positive type (clone (155-4-213), in which the antigens increased soon (5 h) after seeding at 37 degrees c, b ...19816264119
molecular genetics of herpes simplex virus. vi. characterization of a temperature-sensitive mutant defective in the expression of all early viral gene products.the herpes simplex virus 1 (hfem) mutant tsb7 failed to express any detectable viral polypeptides and did not significantly inhibit host cell protein synthesis in infected cells maintained at the nonpermissive temperature. the mutant could complement the growth of a coinfecting temperature-sensitive mutant virus differing in plaque phenotype and thus appeared capable of penetrating doubly infected cells. the yield of tsb7 was enhanced by the coinfecting virus but not to the extent that the coinf ...19816264126
transformation of rodent cells by a cloned dna fragment of herpes simplex virus type 2.transformation of rodent cells with isolated restriction endonuclease fragments of herpes simplex virus type 2 dna identified a region of the genome located between map positions 0.58 and 0.62. these sequences were cloned into pbr322, and the recombinant plasmid was used to transform primary rat embryo cells and nih 3t3 cells. the transformants were selected for their ability to form dense foci on a monolayer or to form colonies in semisolid medium. in contrast to the parental rat or mouse cells ...19816264141
mechanism of degradation of duplex dna by the dnase induced by herpes simplex virus.reaction intermediates formed during the degradation of linear pm2, t5, and lambda dna by herpes simplex virus (hsv) dnase have been examined by agarose gel electrophoresis. digestion of t5 dna by hsv type 2 (hsv-2) dnase in the presence of mn(2+) (endonuclease only) gave rise to 6 major and 12 minor fragments. some of the fragments produced correspond to those observed after cleavage of t5 dna by the single-strand-specific s1 nuclease, indicating that the hsv dnase rapidly cleaves opposite a ni ...19816264148
risk of recurrence after first episodes of genital herpes. relation to hsv type and antibody response.to define risk factors associated with recurrent genital herpes-simplex-virus infection caused by either type 1 or 2 herpesvirus (hsv-1 or hsv-2), we prospectively studied 137 patients with a first symptomatic episode of the disease and 87 with a recurrent episode. first episodes were divided into 78 primary infections (no antibodies to hsv in acute-phase serum) and 59 nonprimary infections (antibodies present). hsv-1 infections were less frequent and less likely to recur than hsv-2 infections. ...19816264300
characterization of rat embryo cells transformed by ts mutants and sheared dna of herpes simplex virus types 1 and 2 and a derived tumor cell line.four independent isolated lines transformed by ts mutant or sheared dna of herpes simplex virus types 1 and 2 and one tumor cell line were characterized according to their behavior in assays of aggregation, growth in low serum, doubling times, saturation density, agglutination by lectins, uptake of metabolites, and production of plasminogen activator. the criteria used to select the transformed cells used in this study were the formation of altered foci of cells followed by the formation of a co ...19816263467
induction of natural killer cells by herpes-simplex virus type 2 in resistant and sensitive inbred mouse strains.infection of mice with herpes simplex virus type 2 (hsv 2) stimulated natural killer (nk) cells in a variety of inbred mouse strains including athymic nude mice. essentially all mouse strains tested exhibited high nk activity on day four after virus inoculation. assayed 24 hours after infection, swr/j, akr/j, sjl/j and c57b1/10j mice were low or negative for these non-virus-specific cytotoxic responses. whereas the first two mouse strains were most sensitive to the lethal effects of hsv 2, the l ...19816263793
assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera.a reliable and reproducible method for determining specific reactivity to herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in human sera has been developed. human sera were used to immunoprecipitate hsv-specific glycoprotein antigens from both hsv-1- and hsv-2-infected cell extracts. the viral glycoproteins precipitated from these extracts were then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to detect specific reactivity of the sera with distinct type-specific anti ...19816262247
production of hybrid cell lines secreting antibodies to herpes simplex virus type 2.following different immunization schedules we have produced 102 myeloma--spleen cell hybrid lines (30.8% of the total) secreting antibodies to hsv-2 infected cells. the highest yield of hybrids was derived from fusion of myeloma cells with spleen cells from mice sensitized by footpad injection of formalin-inactivated virus and boosted intravenously with the same antigen. to date, twelve of the antibody-producing hybrids have been cloned by limiting dilutions to generate over 100 monoclonal lines ...19816262346
involvement of an early human cytomegalovirus function in reactivation of quiescent herpes simplex virus type 2.we have previously described an in vitro system in which the function lacking for herpes simplex virus type 2 (hsv-2) replication can be induced by human cytomegalovirus (hcmv). the mechanism of this reactivation of quiescent hsv-2 by hcmv has been further defined. the hcmv function(s) responsible for hsv-2 stimulation was examined temporally, and the fraction of cells in quiescent cultures producing hsv-2 after superinfection was determined. using independent biological, genetic and molecular t ...19816262523
immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.in this paper we report that viral polypeptides from herpes simplex virus 1 (hsv-1) and 2 (hsv-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. the polyvalent hyperimmune sera against hsv-1 reacted with 17 hsv-1 polypeptide bands and 8 hsv-2 polypeptide bands. concordantly, polyvalent sera against hsv-2 ...19816260673
molecular genetics of herpes simplex virus. vii. characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in dna synthesis and accumulation of gamma polypeptides.we report on the properties of a temperature-sensitive mutant produced by transfection of cells with intact dna and a specific dna fragment mutagenized with low levels of hydroxylamine. the plating efficiency of the mutant at 39 degrees c relative to that at 33.5 degrees c was 5 x 10(-6). the pattern of polypeptides produced at the nonpermissive temperature was similar to that seen with wild-type virus in infected cells treated with inhibitory concentrations of phosphonoacetic acid in that alpha ...19816260973
repair replication of viral and cellular dna in herpes simplex virus type 2-infected human embryonic and xeroderma pigmentosum cells. 19816261452
mode of action of phosphonoformate as an anti-herpes simplex virus agent.phosphonoformate inhibited the replication of herpes simplex virus (hsv) type 1 and type 2 in culture. the concentration required to inhibit the replication of both types of virus by 2 logs at 28 h post-infection was approximately 150 microm. it was more potent than phosphonoacetate against the growth of both virus types. a virus mutant which is resistant to phosphonoacetate was cross-resistant to phosphonoformate. arsonoacetate, at 300 microm, had no antivirus activity. phosphonoformate also in ...19816260189
tumorigenicity of herpesvirus-transformed cells correlates with production of plasminogen activator.our studies first demonstrated that established hamster cell lines transformed in vitro by herpesviruses activate plasminogen more effectively than normal hamster fibroblasts. this ability is probably due to increased levels of the enzyme plasminogen activator (pa). in the studies described here, the 333-8-9 cell line, originally transformed by herpes simplex virus type 2 strain 333, was used to derive subclonal lines that maintained stable pa phenotypes over the course of long in vitro passage. ...19816100964
herpes simplex virus types 1 and 2 and multiple sclerosis.a hypothesis is proposed which states that multiple sclerosis (ms) is caused by herpes simplex virus type 2 (hsv-2) in persons lacking herpes simplex virus type 1(hsv-1) immunity. it is examined by comparing the epidemiology of these viruses with that of ms. the age at which hsv-2 infections are first detected, as well as the age of peak incidence are similar to those for ms. if age-specific hsv-1 immunity data from various places is compared, an inverse relationship of immunity levels with lati ...19816116906
pathogenesis of herpes simplex virus infections in guinea pigs.the pathogenesis of herpes simplex virus types 1 and 2 has been studied in guinea pigs after inoculation by various routes (subcutaneous and intradermal infection in footpads and vaginal infection). clinical observations as well as virus isolation studies are reported. herpes simplex virus type 2 infection by all three routes of inoculation led to acute primary and recurrent lesions. virus persisted in the nervous system, particularly in sensory ganglia, and locally at the site of inoculation. h ...19816277787
monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein icp 4.monoclonal antibodies were prepared against herpes simplex virus type 1 (strain 14012) by two immunization procedures. procedure a utilized infectious virus propagated in mouse cells, and procedure b utilized mouse cells infected with herpes simplex virus in the presence of cycloheximide and harvested 1 h after removal of the inhibitor. a total of 52 monoclonal antibodies were obtained against 10 herpes simplex virus proteins, including four glycosylated proteins (a 110,000-molecular-weight prot ...19816277788
cell-mediated immunity to herpes simplex virus: recognition of type-specific and type-common surface antigens by cytotoxic t cell populations.in this communication, we examine the specificity of anti-herpes simplex virus (hsv) cytotoxic t lymphocytes (ctl). serological studies of the two related hsv serotypes (hsv-1 and hsv-2) have revealed both type-specific and cross-reactive antigenic determinants in the viral envelope and on the surface of infected cells. by analysis of cytotoxicity of ctl, generated in vitro by restimulation of splenocytes from mice primed with one or the other hsv serotype, the recognition of both type-specific ...19816277790
antibody responses in humans to individual proteins of herpes simplex viruses.sera from 231 women were used to examine their frequency of precipitation of various herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) proteins and to determine if there was a rank order of immune responsiveness of humans to these hsv antigens. radiolabeled viral proteins were reacted with serum and immune complexes isolated with staphylococcal protein a. individual antigens were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. as a group, these sera precipitated ...19816277791
linkage map of cotransfected viral dna sequences in a mouse 3t3 cell line.a physical map was constructed of a cotransformed 3t3 thymidine kinase (tk) negative cell line revealing a close linkage of the selectable herpes simplex virus type 2 tk gene and cotransfected simian virus 40 dna sequences in high-molecular-weight cellular dna.19816277824
microscopic examination of the intracellular fate of infectious herpes simplex virus dna.the intracellular fate of 3h-labeled exogenous herpes simplex virus type 2 dna following the exposure of recipient cells to selected dna facilitators and the ultrastructural changes which ensued in transfected cultures were examined. in most cases, electron microscopic radioautography demonstrated that exogenous dna reached the nucleus within 1 h postinoculation. at later time periods, enlargement of nuclei and margination of chromatin were noted. cells treated with polyornithine differed signif ...19816276330
[use of a contraceptive suppository as chemoprophylaxis against sexually-transmitted diseases].the incidence of sexually transmitted diseases (std), especially gonorrhea, has risen dramatically in the past 2 decades. the population at greatest risk is also at high risk for unwanted pregnancies. since currently employed treatment methods are failing to curb the problem, a search was instituted for an available vaginal contraceptive that would have a prophylactic effect against these diseases. in vitro studies have indicated that a vaginal contraceptive made of phenylmercuric acetate is ...19816277015
sexually transmitted infections and cervical atypia.epidemiologic studies have long associated cervical cancer and dysplasia with sexual activity. in the past two decades an abundance of biological and epidemiologic data have indicated a relationship between herpes simplex virus type 2 (hsv-2) and these conditions. unfortunately, the strength of the evidence (albeit circumstantial) has led many researchers to focus exclusively on the putative role of hsv-2 as the carcinogen. at the same time, many studies have shown that cervical atypia, if it is ...19816277023
[new antigen induced by herpes simplex virus in human tumors].the study showed the new antigen induced by herpes simplex virus type 2 (hsv-2) to be present not only in cancer tumors of the cervix and corpus uteri, and ovaries but also in malignant tumors of the mammary glands, kidneys, urinary bladder, as well as in tissues of fibrous-cystic mastopathy and fibroadenoma of the mammary glands. in malignant tumors of the cervix, however, the hsv-2-induced antigen was found more frequently and had a higher serological activity than in other tumors. in contrast ...19816278776
[malignant transformation of newborn hamster cells by herpes simplex virus type 2].the oncogenic potentials of hsv-2 inactivated by ultraviolet rays in newborn hamster cell culture were studied. virus-induced morphological and malignant transformation of cells was accompanied by synthesis of virus-specific antigen, changes in morphology, formation of colonies in semi-liquid agar, and tumorigenicity of cells. the animals bearing tumors induced by transformed cells showed humoral and cellular immune responses to the virus-specific antigen. lines of transformed and tumor cells we ...19816278782
antiherpes activity of [e]-5-(1-propenyl)-2'-deoxyuridine and 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil.5-(1-propenyl)-1-beta-d-arabinofuranosyluracil has been synthesized, and this compound and [e]-5-(-propenyl)-2'-deoxyuridine have been tested for inhibition of herpes virus multiplication. only [e]-5-(1-propenyl)-2'-deoxyuridine was found to be an active inhibitor reducing by 50% the plaque formation of herpes simplex virus type 1 (hsv-1) at about 1 mum. a comparison to the bromovinyl derivatives showed the following order of descending activity; [e]-5-(2-bromovinyl)-2'-deoxyuridine greater than ...19816280606
a comparison of phosphonoacetic acid and phosphonoformic acid activity in genital herpes simplex virus type 1 and type 2 infections of mice.the activity of phosphonoacetic acid (paa) and phosphonoformic acid (pfa) against four strains of herpes simplex virus type 1 (hsv-1) and four strains of hsv-2 were compared in tissue culture and in a murine model of genital herpes. in mouse embryo fibroblast cells, both drugs were three-fold more active against the hsv-1 strains than against the hsv-2 strains. in contrast, in the animal model infections, paa appeared to be more active against the hsv-2 strains, while pfa was equally effective a ...19816280607
the development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses.two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of mrc-5 and vero cells, and herpes simplex type 2 virus. cell growth was similar in both systems giving yields of 14 x 10(5)/cm2 in microcarrier and 18 x 10(5)/cm2 in glass sphere cultures with yields of vero cells being marginally better than mrc-5 cells. virus yields were only slightly lower in these cultures than in small scale stationary cultures ...19816281104
herpes simplex vaccine using cell membrane associated antigen in an animal model.vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of herpes simplex virus type 2 (hsv-2) infected vero cells with dithiothreitol and formaldehyde. these preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with hsv-2.19816281105
expression of herpes simplex virus common surface antigens and malignancy by clonal cells of a herpes simplex virus type 2-transformed line.herpes simplex virus (hsv) type common surface antigens (csa) were examined by indirect immunofluorescence with rabbit antiserum to hsv type 1 in a clonal hamster cell line 155-4 transformed by hsv type 2. the tumor formation was examined in hamsters transplanted with various transformed and tumor cells. the examination of subclones derived from 1554-cell line gave the following results. (1) thirty subclones were isolated and classified into three phenotypes as to csa expression: (i) in csa-pos ...19816281115
function, target-cell preference and cell-surface characteristics of herpes-simplex virus type-2-induced non-antigen-specific killer cells.wild-type and congenitally athymic nude mice injected with herpes-simplex virus type 2 (hsv 2) responded with a local outburst of non-antigen-specific killer cells masking any virus-specific response. cytolytic activity could be assayed on mouse-tumor cell lines and on syngeneic or allogeneic non-transformed cells from various sources. some of the tumor cell lines and proteose-peptone-induced peritoneal exudate cells were lysed more efficiently after infection with either hsv 2, vaccinia or infl ...19816165679
comparison of the effects of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate against herpes simplex virus, varicella-zoster virus, and cytomegalovirus with their effects on cellular deoxyribonucleic acid synthesis.in a single line of human foreskin fibroblasts, minimum inhibitory concentrations (mics) and the minimum intracellular virus inactivation concentrations (miics) of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate were assayed for a number of recent isolates of herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv), and cytomegalovirus (cmv). (the term miic is used here to describe the selective qualitative intracellular inhibition of the ...19816166244
detection of rna complementary to herpes simplex virus dna in human cervical squamous cell neoplasms.nonneoplastic and neoplastic cervical biopsy specimens were examined by in situ hybridization to 125i-labeled dna of herpes simplex virus (hsv), adenovirus, and bacteriophage lambda dna's, and quantitative hybridization data were obtained using a video image analyser. hsv-specific rna was detected in 72% of cervical intraepithelial neoplasia, 60% of squamous cervical carcinomas, 2% of nonneoplastic cervices, and 9% of primary adenocarcinomas of the cervix. none of the tissues gave positive hybri ...19816167349
monoclonal antibodies to two glycoproteins of herpes simplex virus type 2.monoclonal antibodies to herpes simplex virus type 2 were found to precipitate different numbers of radiolabeled polypeptides from lysates of virus-infected cells. antibodies directed against two viral glycoproteins were characterized. antibodies from hybridoma 17 alpha a2 precipitated a 60,000-molecular-weight polypeptide which chased into a 66,000- and 79,000-molecular-weight polypeptide. all three polypeptides labeled in the presence of [3h]glucosamine and had similar tryptic digest maps. the ...19816168775
characteristics of a persistent rubella infection in a human cell line.a persistent infection of the human mcf-7 cell line (mcf-7rv) was established with the dbs strain of rubella virus at a low multiplicity of infections. fluorescent antibody staining revealed that 100% of the cells were positive for rubella antigens. the infected cells were refractory to superinfection with vesicular stomatitis virus (vsv) but were susceptible to herpes simplex virus type 2 (hsv-2). no interferon could be detected in infected cell culture fluid, and continuous passage in the pres ...19816169785
herpes simplex virus type 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis.intracellular p40 is a class of protein ranging in molecular weight from 39,000 to 45,000 that is immunoprecipitated from herpes simplex virus type 1 (hsv-1)- and hsv-2-infected cell extracts by mouse monoclonal antibodies or guinea pig antisera against hsv-1 and hsv-2 nucleocapsid p40. analysis by a two-dimensional gel system showed that hsv-1 and hsv-2 intracellular p40 each consisted of three major components. however, these hsv-1 and hsv-2 proteins differed in charge and size. analysis of st ...19816172597
[incidence of herpes virus (hsv-2) alterations in squamous cells of vagina epithelium (author's transl)].the authors have analysed 17,519 cytological cervix smears. alterations characteristic of hsv infection were recorded from squamous cells of vaginal and cervical epithelia in 21 cases (0.12 per cent). incidences of cytologically detected hsv infection of the vagina and cervix were 0.17 per cent among female factory workers and 0.08 per cent among peasant women. no statistically unambiguous difference was found to exist between the incidences of genital hsv infections in the two groups examined ( ...19817198853
a study of hla and drw antigens in severe recurrent herpes progenitalis (hsv-2) infection.in this preliminary study the phenotypic distribution of hla and drw antigens was investigated in patients with recurrent herpes progenitalis. ninety-three white men between the ages of 20 and 53 years, with at least a 2-year history of recurrent herpes progenitalis infection, and 558 age-matched white men from the same area of los angeles were studied by means of a microcytotoxicity assay. statistical analysis of data indicated that there is no increased incidence of any hla antigen. the b cell ...19826954168
herpes simplex virus diagnosis. antigen detection by elisa and flow microfluorometry.rapid diagnosis of herpes virus (hsv) infection is essential, primarily in the clinical management of the obstetrical patient. we developed an elisa procedure for viral antigen detection within 24 hours. the assay is sensitive and specific, detecting as little as 2 x 10(-3) micrograms antigen protein and reactivity with mock antigen or unrelated viruses (not observed). good (91.6%) correlation is observed between elisa and virus isolation. however, direct quantitative comparison indicates that t ...19826762291
effect of monoclonal antibodies on limited proteolysis of native glycoprotein gd of herpes simplex virus type 1.we examined the properties of 17 monoclonal antibodies to glycoprotein gd of herpes simplex type 1 (hsv-1) (gd-1) and hsv-2 (gd-2). the antibodies recognized eight separate determinants of gd, based on differences in radioimmuno-precipitation and neutralization assays. the determinants were distributed as follows: three were gd-1 specific, one was gd-2 specific, and four were type common. several type-specific and type-common determinants appeared to be involved in neutralization. we developed a ...19826176725
protease inhibitors suppress fibrinolytic activity of herpesvirus-transformed cells.previous studies in this laboratory have quantified the fibrinolytic activity of herpesvirus-transformed cell lines and implicated the proteolytic capacity of cloned cell lines in the formation of primary and metastatic tumours. because of the involvement of proteases in tumourigenesis in this system, we examined the effect of various protease inhibitors (alpha-1-antitrypsin, leupeptin and alpha-2-macroglobulin), as well as hamster serum containing acute phase proteins generated in response to p ...19826178796
anti-herpesvirus activity of adenine arabinoside analogues in tissue culture and a genital infection of mice and guinea pigs.four analogues of adenine arabinoside (ara-a) were compared for activity against herpes simplex virus (hsv) in tissue culture and in a genital infection of mice and guinea pigs. these analogues, 5'-monophosphate (ara-amp), 5'-valerate ester (ara-av), 2'3'-diacetate ester (ara-ada), and 2',3',5'- triacetate ester (ara-ata) have greater water and lipid solubility and resistance to deamination than ara-a. in mouse embryo fibroblast cells, similar viral inhibitory levels were noted with ara-a, amp, ...19826179469
effect of combinations of acyclovir with vidarabine or its 5'-monophosphate on herpes simplex viruses in cell culture and in mice.the combination of various concentrations of acyclovir and vidarabine or its 5'-monophosphate usually produced an additive interaction with various strains of herpes simplex virus types 1 and 2 in vero cells. similarly, certain combinations of these drugs were more effective than the individual drugs in decreasing the mortality and increasing the mean day of death of mice inoculated intracerebrally with herpes simplex virus type 2. neither antagonism nor interference was noted for any of the in ...19826182836
herpes simplex infection simulating a positive auto-inoculation for haemophilus ducreyi.auto-inoculation from a genital ulcer suspected of being ulcus molle gave redness after 24 hours and after 48 hours vesicles and pustules appeared. cultivation from the auto-inoculation after the 48 hours was positive for herpes simplex virus type 2. our observation underlines two points: auto-inoculation for the diagnosing of haemophilus ducreyi infection may be mimicked by herpes simplex infection, and the incubation period of herpes simplex can be shorter than the 4-5 days usually given.19826183910
experimental infection of inbred mice with herpes simplex virus. iv. comparison of interferon production and natural killer cell activity in susceptible and resistant adult mice.inbred mouse strains differ in susceptibility to infection with herpes simplex virus type 1 or type 2 (hsv-1, hsv-2). in this study interferon production was tested in the peritoneal exudate of mice after intraperitoneal (i.p.) injection of hsv-1 or hsv-2. in hsv-resistant mice (c57 bl/6, c3 h/hej) high titers of interferon were already present 2 to 4 hours after injection. in comparison, less resistant mice (dba/2, akr) lacked this early response. there was no correlation between interferon tit ...19826187314
control of expression of the herpes simplex virus-induced deoxypyrimidine triphosphatase in cells infected with mutants of herpes simplex virus types 1 and 2 and intertypic recombinants.infection of cells with herpes simplex virus type 1 (hsv-1) induces high levels of deoxypyrimidine triphosphatase. the majority of the enzyme activity is found in infected cell nuclei. a similar activity is induced by hsv type 2 (hsv-2) which, in contrast to the hsv-1 enzyme, fractionates to more than 99% in the soluble cytoplasmic extract. of a series of temperature-sensitive mutants of hsv-1 studied, only the immediate-early mutants in complementation group 1-2 (strain 17 mutants tsd and tsk a ...19826128430
detection of rna complementary to herpes-simplex virus in mononuclear cells from patients with behçet's syndrome and recurrent oral ulcers.viral dna probes were used in in-situ hybridisation to detect the complementary rna in mononuclear cells from the blood of normal subjects and patients with behçet's syndrome and recurrent oral ulcers. hybridisation of 125i-labelled dna probes was detected by means of autoradiography and quantitated with a video image-analysis technique. hybridisation between herpes-simplex virus type 1 (hsv-1) dna and the complementary rna in mononuclear cells was significantly greater in 10 of 20 patients with ...19826129461
limited transcription of the herpes simplex virus genome when latent in human sensory ganglia.human paravertebral ganglia were examined for the presence of specific sequences of viral rna by in situ cytological hybridization, using as probes individual fragments of herpes simplex virus type 2 dna. ganglia were obtained at autopsy from 40 patients, and 14 were positive for herpes simplex virus rna. transcripts from the left-hand 30% of the viral genome were detected in all of the herpes simplex virus-positive cases. rna homologous to other sequences from the l component were present less ...19826281477
sex partners and herpes simplex virus type 2 in the epidemiology of cancer of the cervix.the authors examined the interaction of exposure to various numbers of sex partners and evidence of antibodies to herpes simplex virus type 2 (hsv-2) in cervical neoplasia in 181 cases of cervical dysplasia, carcinoma in situ and cancer of the cervix and 130 control patients in los angeles county, california hospitals, in 1974-1979. studies by the authors and other investigators have found that risk of cancer of the cervix was enhanced with numbers of sex partners, frequency and duration of usin ...19826282116
enhanced tumor growth in vivo by a factor in human platelets and rat liver.liver cell supernatant and platelet extract can promote the in vivo growth of herpes simplex virus type 2-transformed hamster embryo fibroblast cells. delineation of necessary growth factors for tumor cells in vivo may open up new strategies to inhibit malignant cell growth.19826280855
chemical interactions with herpes simplex type 2 virus: enhancement of transformation by hydrazine and 1,2-dimethylhydrazine.quantitative assays for the morphological transformation of 3t3 swiss mouse cells by herpes simplex type 2 virus (hsv-2) were employed to examine the effect on cell transformation of chemical carcinogens and suspected carcinogens. exposure of the cells to the chemical compound, followed by virus infection, resulted in enhancement of transformation when compared to that observed with chemical or virus alone. enhancement occurred in tests utilizing either uv light-inactivated hsv-2 (strain 333) or ...19826280884
efficacy of (e)-5-(2-bromovinyl)-2'-deoxyuridine against different herpes simplex virus strains in cell culture and against experimental herpes encephalitis in mice.(e)-5-(2-bromovinyl-2'-deoxyuridine (brvudr) showed strong antiviral activity against different laboratory strains and clinical isolates of herpes simplex virus type 1 (hsv-1) on primary rabbit testes (prt) cells with a 50% inhibition of plaque formation (id50) at 0.01-0.02 microm. one laboratory strain (hsv-1-s), however, was completely refractory even at concentrations as high as 100 microm. in contrast, the id50s for all herpes simplex virus type 2 (hsv-2) strains were about 10(2) - 10(3) tim ...19826295273
restriction endonuclease analysis of dna from genital isolates of herpes simplex virus type 2.high-resolution restriction endonuclease analysis of dna from multiple herpes simplex virus type 2 isolates from 30 patients at a single clinical center has indicated that herpes simplex virus type 2 dna sequences are highly conserved. however, the use of six endonucleases, ecori, bglii, hindiii, kpni, bamhi, and ssti, established that each patient was infected by a unique virus. comparison of virus isolates from the same patient show that the most sequence variation occurred in the terminal and ...19826295948
virus-induced thymidine kinases as markers for typing herpes simplex viruses and for drug sensitivity assays.a rapid, reproducible and objective new method for typing herpes simplex viruses type 1 (hsv-1) and type 2 (hsv-2) based on the effects of virus-induced thymidine kinases on various antiviral drugs has been developed. when several laboratory strains and clinical isolates were typed by this method and compared to the results obtained by the immunofluorescence antibody typing method, agreement was found for all the viruses. the new technique has the added advantage of determining the sensitivity o ...19826296179
monoclonal antibodies to three non-glycosylated antigens of herpes simplex virus type 2. 19826296279
neurological involvement in mice after infection with a cold-adapted herpes simplex type 2 virus.experimental intracerebral infection of 4-week-old mice with the ms strain of herpes simplex virus type 2 or its derivative cold variant was compared. the infectious process was followed in both the brain tissue and the trigeminal ganglia, using hematoxylin and eosin and antigenic tracing with indirect peroxidase-antiperoxidase staining. the wild-type virus elicited a severe meningitis and necrotic lesions by 7 days post-inoculation in the brain. the cold variant produced a mild meningitis and n ...19826279512
typing of herpes simplex virus with type-specific human immunoglobulin m in an indirect immunofluorescence assay.sera from nine individuals with suspected primary herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection were screened to identify those containing hsv type-specific immunoglobulin m (igm). selected sera were then utilized in an igm-specific indirect immunofluorescent-antibody hsv-typing assay (patent pending). to evaluate the procedure, 29 hsv isolates were grown in cultures of continuous human amnion cells, fixed, and used as substrates for indirect immunofluorescence. determination o ...19826279692
inhibiting herpes simplex virus type 2 infection in human epithelial cells by gossypol, a potent spermicidal and contraceptive agent.gossypol, a male contraceptive, has an antifertility effect of 99.9%. it has also been reported to possess antiviral activities, and to inhibit herpes simplex virus infection in mice. dorsett and kerstine found that gossypol inactivated the infectivity of the enveloped virus parainfluenza type 3 and herpes simplex for hep-2 carcinoma cells but had no effect on the infectivity of the nonenveloped poliovirus. the authors studied the effect of gossypol on infection by herpes simplex virus type 2 ...19826277198
viral receptors on isolated murine and human ependymal cells.viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. we report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. with the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. mumps virus, measles virus, parainfluenza type 3, and herpes simplex v ...19826276976
sulphated glycoproteins induced by herpes simplex virus.bhk cells infected with strain 17 syn+ (hsv-1) or hg52 (hsc-2) incorporated inorganic sulphate into polypeptides which co-migrated on sds-polyacrylamide gels with virus-induced glycoproteins. the major sulphated glycoprotein was glycoprotein e. in addition, less-intense sulphated bands co-migrated with glycoprotein d and hsv-1 glycoprotein a/b/c. sulphate label co-migrating with hsv-2 glycoprotein a/b/c was occasionally observed. we have investigated which sulphated polypeptides are excreted fro ...19826278062
the effect of a temperature-sensitive lesion in the alkaline dnase of herpes simplex virus type 2 on the synthesis of viral dna. 19826278703
activation of herpes simplex virus (hsv) type 1 genome by temperature-sensitive mutants of hsv type 2.previous studies have demonstrated that herpes simplex viruses (hsv) type 1 (hsv-1) and type 2 (hsv-2) can be maintained in a repressed form in human embryo lung cells. reducing the incubation temperature or superinfecting with a heterologous herpesvirus, human cytomegalovirus (hcmv), results in activation of virus replication. we now report that superinfection with a partially homologous herpesvirus, hsv-2, also resulted in activation of hsv-1. to minimize excessive synthesis of infectious hsv- ...19826278723
synergism among biolf-62, phosphonoformate, and other antiherpetic compounds.9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine (biolf-62) is highly synergistic with either phosphonoformate or phosphonoacetate when used in combination against herpes simplex virus types 1 and 2 in vitro. acycloguanosine did not show significant synergism with these two compounds. bromovinyldeoxyuridine and phosphonoformate were highly synergistic against herpes simplex virus type 2, but not against type 1.19826297382
properties of a herpes virus-transformed hamster cell line--i. growth and culture characteristics of sublines of high and low metastatic potential.a herpes virus hominis type 2-induced tumour line of the syrian hamster, designated hsv-2-333-2-26, exhibited a low level of spontaneous metastasis from the primary subcutaneous tumour mass. two lung foci, met a and met b, were resected and transplanted s.c.; both lines showed an elevated metastatic potential compared with the parent cell line such that all animals inoculated with these cells developed metastatic disease within 40 days after resection of the primary tumour. neither the rapidity ...19826297914
inapparent genital herpes simplex virus infection in college women.during a six-month period, 600 gynecological samples were collected from 585 women with typical herpes lesions, women with non-herpes symptoms (ie, vaginitis, moniliasis, trichomoniasis, etc), and normal women seen at the student health center gynecological clinic and processed for herpes simplex virus (hsv) isolation. from these specimens, 29 samples from 25 of the 585 women (4.3%) were positive for hsv. when these isolates were typed using plaque diameter in chick cells, heat stability of vira ...19826298359
characterization of an herpes simplex virus type 2 mutant, which is resistant to acycloguanosine and causes fusion of bsc1 cells.a mutant of herpes simplex virus type 2, which induces low levels of thymidine-kinase activity in infected bsc1 cells and consequently able to grow in the presence of acycloguanosine, was isolated. this mutant has also been shown to cause fusion of bsc1 cells. in bsc1 cells, co-infected with the wild-type strain and the mutant, the yield of each of the two viruses was normal but the rounding and aggregation of cells observed, resembled that found in wild-type infected cultures. when the mixed in ...19826299232
imide derivatives of 3-nitro-1.8-naphthalic acid: their inhibitory activity against dna viruses.the antiviral action of a new drug, 5-amino-2-(dimethylaminoethyl)-benzo-[de]-isoquinolin-1.3-dione has been studied against herpes simplex type 2 (hsv-2) and adenovirus type 5 (ad-5) grown in vero cells. the concentration of the drug which gives a 5 log10 reduction in virus titer was 4 micrograms/ml (maximum tolerated concentration) for hsv-2. the anti-hsv-2 activity of this compound was one log. more powerful than that of iododeoxyuridine (idu). at this concentration the drug shows virucidal a ...19826299235
abortive infection of neural cells by herpes simplex virus type 2.the nature of the refractoriness of c6 rat glioma cells to herpes simplex virus type 2 (hsv-2) was examined. infection of c6 cells with hsv-2 results in low virus yields, not exceeding the input virus. although virus growth studies suggested a restricted cycle of virus replication, synthesis of hsv-2 dna and hsv-2-specific antigens could not be detected. in addition, hsv-2 yields in c6 cells were unaffected by interferon, cycloheximide, tunicamycin, actinomycin d and cytosine arabinoside. howeve ...19826299239
modification of herpes 2-transformed cell-induced tumors in mice fed different sources of protein, fat and carbohydrate.the effects of different sources of protein (milk, soy, wheat, fish and beef), fat (corn oil and butter), and carbohydrate (dextrin and sucrose) on tumor development and on spleen characteristics were investigated in balb/c mice injected subcutaneously with 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells). low or high levels of protein and fat were used. several weeks post-injection results indicated that a high level of fat significantly enhanced tumor incidence. a high fat ...19826299511
different weekly changes in immune response in virus-transformed cell-injected mice fed two different diets.changes in tumor development and in certain immune responses were investigated at 7-weekly intervals after subcutaneous injection of 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells) into male balb/c mice fed 2 different diets. one diet contained 11% casein and 5% fat while the other had 11% supplemented wheat gluten and 30% fat. weanling mice (140/group) were fed one or the other of the diets for 12 weeks before injection and subsequent testing of 15 injected and 5 non-inject ...19826299512
herpes virus-related antigens in herpes simplex virus type 2-transformed cells in the course of cervical carcinoma.anticomplement immunofluorescence (acif) technique was applied to herpes simplex virus type 2 (hsv-2)-transformed cells (333-8-9) and to cells lytically infected with hsv-2 in sera from women with cervical carcinoma. there was a correlation between the positive results with both types of cells. long-term survivors suffering from cervical cancer showed a high percentage of positive immunofluorescence (if) reactions in hsv-2 transformed cells (77%) as well as in cells lytically infected with hsv-2 ...19826299751
use of monoclonal antibodies to hsv-1 and hsv-2 for serological analysis of the viral glycoproteins.herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) specify four major glycoproteins designated ga/gb, gc, gd and ge. type-specific and cross-reacting monoclonal antibodies to the viral glycoproteins have been used to probe their antigenic determinants. our studies show the following: (i) type-specific monoclonal antibodies are suitable reagents for serotyping isolates. discriminating monoclonal antibodies distinguish antigenic variants among the strains of each serotype. (ii) glycoproteins ga ...19826299824
development of sensitive and enzyme-linked immunoassays for herpesvirus antigens and some applications.sensitive enzyme-linked immunoassays for herpes simplex virus (hsv), cytomegalovirus (cmv) and varicella-zoster virus (vzv) were developed. both a sandwich technique, using antiviral antibodies from two species to detect the antigen, and an inhibition assay where the sample antigen was incubated with one antiserum, could be used. around 4-50 ng of viral antigens (measured as protein content) could be detected. the elisa inhibition technique using type-specific antisera could differentiate betwee ...19826299833
application of molecular genetics to the design of live herpes simplex virus vaccines.in principle, several avenues for attenuation of herpes simplex viruses (hsv) are now available. these include intermixing of hsv-1 and hsv-2 genes by recombination, altering the regulation of gene expression and the deletion of viral genes not required for replication of the virus in permissive cells in culture. results of analyses of hsv-1 x hsv-2 recombinants and of mutants containing a deletion in a gene expressed early in infection showed a loss of virulence when infected by intracerebral r ...19826299836
herpes simplex virus subunit vaccine: characterization of the virus strain used and testing of the vaccine.herpes simplex virus type 1 (hsv-1) used for vaccine production was isolated from a nasal recurrent infection and propagated over a limited number of passages in human diploid cells only. it was designated as hsv-1 bw3 and has been characterized by neutralizing antibodies as a typical hsv type 1 strain. in vitro transformation studies performed with this isolate in mouse or hamster cells revealed only very low, if any, transforming capacity. a preparation of hsv-1 bw3 which can be used as seed l ...19826299841
intertypic superinfection of herpes simplex virus in vivo leads to reisolation of latent viruses with newly acquired lymphotropic properties.the susceptibility of juvenile tupaias to several strains of temperature-sensitive mutants of hsv-1 and 2 was tested. it was found that in all cases the animal survived an infection of 1 x 10(7) pfu of temperature-sensitive mutants which were administered intravenously. in contrast, an infection dose of 1 x 10(3) pfu of wild-type hsv-2 and 1 x 10(2) pfu or hsv-1 was fatal for juvenile tupaias. those animals which had initially been infected with the ts-mutants of hsv-1 and/or 2 were protected ag ...19826299861
the role of herpes simplex virus in cervical and vulvar cancer.this report extends the association of herpes simplex virus type 2 (hsv2) with cervical cancer to vulvar carcinoma. in addition to seroepidemiologic observations linking prior hsv2 infection to genital cancer, the new evidence involves the neoplasms themselves. biopsies of cervical and vulvar cancers were found to contain hsv2-specified antigens. the virus-induced antigens are nonstructural dna-binding proteins normally present in the nuclei of infected cells; however, in the cancer cells they w ...19826299862
effect of acyclovir on genital herpes in guinea pigs.the chemotherapeutic efficacy of acyclovir was evaluated in guniea pigs that were inoculated intravaginally with herpes simplex virus type 2 (hsv-2). acyclovir was administered systemically (50 mg/kg of body weight per day ip) beginning three days after inoculation and continuing for 10-11 days. treatment with acyclovir reduced the incidence of paralysis of the hind limbs, mortality, and the severity and duration of genital lesions but had little effect on the excretion of virus from the genital ...19826282986
similarities and differences in the fc-binding glycoprotein (ge) of herpes simplex virus types 1 and 2 and tentative mapping of the viral gene for this glycoprotein.we performed affinity chromatography and immunoprecipitation experiments to determine whether cells infected with herpes simplex virus type 2 (hsv-2) expressed a glycoprotein that was functionally and antigenically related to the hsv-1 fc-binding glycoprotein designated ge. we found that a protein from extracts of hsv-2-infected hep-2 cells bound specifically to an fc affinity column and that the electrophoretic mobility of this protein in sodium dodecyl sulfate-acrylamide gels was slightly less ...19826283108
multimeric forms of herpes simplex virus type 2 glycoproteins.herpes simplex virus type 2-specific glycoproteins present in detergent extracts of infected cells were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under conditions designed to permit detection of multimeric forms of these glycoproteins. two high-molecular-weight glycosylated species were detected when samples were disrupted at lower temperatures or in the absence of any reducing agents. one multimer having an apparent molecular weight of 275,000 was identified as a mul ...19826283127
detection of herpes simplex virus type 2 glycoproteins expressed in virus-transformed rat cells.rat embryo fibroblasts transformed by herpes simplex virus type 2 (hsv-2) were assayed for the expression of certain virus-specific glycoproteins on the surface membranes. monospecific antisera to hsv-2-specific glycoproteins, designated gagb, gc, and gx, were used in membrane immunofluorescence studies with hsv-2-transformed cell lines tref-g-1, tref-g-2, and a tumor-derived rat fibrosarcoma cells line produced in syngeneic rats inoculated with tref-g-1 cells. analysis of the three hsv-2-transf ...19826283145
identification of proteins encoded by a fragment of herpes simplex virus type 2 dna that has transforming activity.cloned bglii fragment n (map units 0.58 to 0.625) of herpes simplex virus type 2 dna has been shown to transform rodent cells to an oncogenic phenotype (galloway and mcdougall, j. virol. 38: 749-760, 1981). rna homologous to this fragment directs the synthesis of five polypeptides in a cell-free translation system. the approximate molecular weights of these proteins are 140,000, 61,000, 56,000, 35,000, and 23,500. the 35,000-dalton protein is the major species late in infection and is the only s ...19826283171
alterations in protein and glycoprotein composition of rat embryo fibroblasts transformed by herpes simplex virus type 2.total and superficially exposed plasma membrane components of tumorigenic herpes-simplex-virus (hsv)-transformed fibroblasts were studied. as a result of oncogenic hsv transformation, a significant decrease of polypeptides 230,000, 180,000, 56,000 and 43,000 daltons was found. these changes were accompanied by a significant increase in superficial exposition of several sialopeptides and two peptides of 30,000 and 15,000 daltons. it was suggested that these two peptides may represent virus-coded ...19826283449
expression of herpes simplex virus type 2 antigens in premalignant and malignant human vulvar cells.vulvar biopsies and explants from patients with vulvitis, hyperkeratosis, condyloma acuminatum, severe dysplasia, and squamous cell carcinoma were screened for herpes simplex virus type 2 (hsv-2) antigens. immunoperoxidase staining for vp143, an early nonstructural polypeptide of hsv-2, was identified within three biopsies exhibiting severe dysplasia, three of seven with carcinoma in situ, and one of three with invasive squamous cell carcinoma. similar staining for vp143 was observed in derived ...19826283892
detection of herpes simplex virus mrna in latently infected trigeminal ganglion neurons by in situ hybridization.latent infection of the trigeminal ganglion with herpes simplex virus type 2 (hsv-2) was studied in guinea pigs by in situ dna hybridization. frozen ganglion sections from animals killed during the period of latent virus infection were studied under nondenaturing conditions. some sections were treated with deoxyribonuclease (dnase) or ribonuclease (rnase) before incubation with hsv dna probes. hsv probes consisted of viral dna nick translated and labeled in vitro with tritiated nucleotides. bact ...19826284019
comparative studies of transformed hamster cells by herpesvirus hominis types 1 and 2.hamster embryo cells transformed by herpesvirus type 2 (hsv-2) virus (two separate cell lines) and by hsv-1 virus (one cell line) were inoculated into hamsters. the morphological appearance of tumours was studied by both light and electron microscopy, and the results compared to similar studies of tumours induced by sv40 virus. the tumours were also tested for immunogenicity, and for susceptibility to contact suppression with bcg. the results indicated that the tumours induced by inoculation of ...19826284192
chemical interactions with herpes simplex type 2 virus: enhancement of transformation by norharman.the effects of the comutagen norharman on herpes simplex virus type 2 growth transformation was studied in 3t3 cells. pre-exposure but not post-exposure of the cells to 0.5 to 10 micrograms of norharman per ml of cell culture media resulted in a nearly three-fold enhancement in transforming activity. these results suggest that certain environmental chemicals may interact with cells creating an increased possibility of transformation by herpes viruses.19826284401
studies on the interrelation of resistance and immunity in a mouse model system of herpes-simplex type 2 infection.intraperitoneal (i.p.) vaccination of mice with attenuated herpes simplex virus type 2 (hsv 2) induced solid protection to i.p. infection with pathogenic virus within two days. protection was non-virus-specific until day four after sensitization but increased in specificity thereafter. normal mice could be protected by adoptively transferred spleen cells, serum, and peritoneal fluid from donors vaccinated seven days before. virus-specific effector cells induced in the spleen by in vivo i.p. sens ...19826284636
characterization of a polypeptide present in herpes simplex virus type 2-transformed and -infected hamster embryo cells.transformation of hamster embryo cells by herpes simplex virus stimulated the production of a 35-kilodalton (35k) protein that was specifically immunoprecipitated, along with other polypeptides, by rabbit hyperimmune serum. this 35k polypeptide was further analyzed by partial digestion with staphylococcus aureus v8 protease in parallel with a 35k polypeptide from herpes simplex virus type 2-infected cells. these polypeptides had almost identical partial-proteolytic cleavage maps, indicating that ...19826284964
isolation and characterization of the growth-stimulating factor in supernatants of herpes simplex virus type 2 infected cells.lytic infection of cv1 cells with herpes simplex virus type 2 does not stimulate ornithine decarboxylase activity and there is no correlation between polyamines and a growth-stimulating factor (gsf) which is present in the supernatant of the cultures. the factor was partially purified by gel filtration on sephadex g-50 and polyacrylamide gel electrophoresis. gel filtration as well as dialysis through membranes with different pore diameters indicated a molecular weight between 3,500 and 10,000 da ...19826285245
herpesvirus-specific rna and protein in carcinoma of the uterine cervix.cloned probes of herpes simplex virus type 2 dna were used in cytological hybridization experiments to detect herpesvirus rna transcripts in the neoplastic cells of tumors of the uterine cervix. virus-specific rna was shown to represent transcription of limited regions of the genome, of which one is known to code for a dna-binding protein that can be found by immunoperoxidase staining in the neoplastic cells of these tumors and has also been detected in cells transformed in vitro by this virus.19826285365
acyclovir treatment of experimental genital herpes simplex virus infections.genital herpes simplex virus type 2 (hsv-2) infections in mice and guinea pigs were used to determine the effectiveness of acyclovir administered topically, orally, or parenterally. topical treatment with 1 percent or 5 percent acyclovir begun six or 24 hours and 5 percent acyclovir begun 48 or 72 hours after intravaginal inoculation of mice with hsv-2 significantly inhibited viral replication in the genital tract. oral administration of acyclovir resulted in a significant reduction in vaginal v ...19826285699
effect of acyclovir on genital infection with herpes simplex virus types 1 and 2 in the guinea pig.the pathogenesis of genital infection with three different strains of herpes simplex virus type 1 (hsv-1) and three strains of herpes simplex virus type 2 (hsv-2) was compared in the guinea pig. strain differences in severity of clinical disease and mortality were noted. hsv-1 strains generally produced milder disease than hsv-2. both hsv-1 and hsv-2 infections resulted in acute and chronic changes in the cervix. virus recovery during latent infection was more frequently obtained from the spinal ...19826285705
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