Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein. | a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ... | 1980 | 6244352 |
| specific viral antigens in rat cells transformed by herpes simplex virus type 2 and in rat tumors induced by inoculation of transformed cells. | a rat cell line, re1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. a high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus thymidine kinase, and there is a correlation between enzyme activity in a tumor and antibody in "tumor s ... | 1980 | 6245808 |
| experimental type 2 herpes simplex ophthalmitis in the newborn rat. | an animal model for the study of type 2 herpes simplex virus (hsv2) ophthalmitis is described. wistar rats were inoculated intracerebrally with hsv2 at 0, 5, 10, 15, 20, or 30 days of age. eyes and brain from all animals, whether they survived or succumbed with encephalitis, were collected for microscopic and virologic studies. up to 20% or more of the hsv2-inoculated rats had lesions in the cornea, uveal tract, and/or retina. herpetic keratiis occurred in a few animals while the eyelids were st ... | 1980 | 6246021 |
| presence of herpes simplex virus type 2 (hsv-2) antibodies and antigens in patients with cervical carcinoma in thailand. | 1980 | 6255050 | |
| some characteristics of an early protein (icp 22) synthesized in cells infected with herpes simplex virus. | in vero cells incubated at 40 degrees c or treated with azetidine at 37 degrees c, synthesis of a polypeptide ('c') of apparent mol. wt. 66000 was stimulated. it was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. in cells infected with herpes simplex virus type 1 (hsv-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide c, was suppressed and infected cell polypeptides icp 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and ... | 1980 | 6245173 |
| different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits. | skin infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ... | 1980 | 6244227 |
| herpes simplex virus phosphoproteins. ii. characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion. | the protein kinase associated with purified herpes simplex virus 1 and 2 virions partitioned with the capsid-tegument structures and was not solubilized by non-ionic detergents and low, non-inhibitory concentrations of urea. the enzyme required mg2+ or mn2+ and utilized atp or gtp. the activity was enhanced by non-ionic detergents and by na+ even in the presence of high concentrations of of mg2+, but not by cyclic nucleotides. the enzyme associated with capsid-tegument structures phosphorylated ... | 1980 | 6252339 |
| collagenase levels in a new model of experimental herpetic interstitial keratitis. | a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ... | 1980 | 6246865 |
| macrophage extrinsic antiviral activity during herpes simplex virus infection. | peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ... | 1980 | 6247426 |
| comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice. | the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ... | 1980 | 6247441 |
| use of [125i]deoxycytidine to detect herpes simplex virus-specific thymidine kinase in tissues of latently infected guinea pigs. | the footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (hsv-2) in both hind footpads. these tissues, shown by our previous study to harbor latent hsv, were dispersed into single cells. the presence of virus-specific thymidine kinase (tk) in these cells was assayed by the uptake and phosphorylation of [125i]deoxycytidine in culture. [125i]deoxycytidine was shown to be a specific ... | 1980 | 6247502 |
| reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls. | serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ... | 1980 | 6254649 |
| [isolation of an herpes simplex virus type 2 from a human cervical cancer by means of a cell cocultivation]. | 1980 | 6247704 | |
| location and cloning of the herpes simplex virus type 2 thymidine kinase gene. | the herpes simplex virus type 2 thymidine kinase gene has been mapped to a position colinear with the herpes simplex virus type 1 thymidine kinase gene and cloned within a 4.0-kilobase fragment in pbr 322. | 1980 | 6245273 |
| oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | 5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ... | 1980 | 6248643 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2. | the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ... | 1980 | 6249191 |
| differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. | fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ... | 1980 | 6249749 |
| genetic retrieval of viral genome sequences from herpes simplex virus transformed cells. | oncogenic transformation of cultured cells by inactivated herpes simplex virus (hsv) types 1 and 2 has been demonstrated. expression of hsv information in these transformed cells has been shown by immunofluorescence studies, detection of hsv neutralizing antibody in sera from tumour-bearing animals and by hybridization of hsv-specific rna. molecular hybridization studies of dna from hsv-2 transformed hamster cells have detected up to 40% of the hsv genome present in several copies. complementati ... | 1980 | 6247659 |
| pathogenicity of wild-type and temperature-sensitive mutants of herpes simplex virus type 2 in guinea pigs. | the pathogenicity of herpes simplex virus type 2 strain 186, the wild-type (wt) strain, and four temperature-sensitive (ts) mutants was studied after genital inoculation of female guinea pigs. infection with the wt virus was generally severe, with extensive skin lesions in 89% and mortality in 37% of inoculated animals. guinea pigs inoculated with ts mutants manifest remarkably mild disease, with lesions occurring in only 16% of the guinea pits and a mortality rate of 7%. wt virus was recovered ... | 1980 | 6254877 |
| temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mrna's. | nuclear and cytoplasmic virus rnas, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus dna fragments generated by bam hi and eco ri restriction endonucleases. polyadenylated immediate early mrnas were separated on denaturing gels containing ch3hgoh giving three virus-specific mrna bands of estimate ... | 1980 | 6253886 |
| cervical carcinoma: detection of herpes simplex virus rna in cells undergoing neoplastic change. | 3h-hsv-2 dna has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. rna complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer. | 1980 | 6249760 |
| anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy. | e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ... | 1980 | 6263181 |
| ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2. | the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ... | 1980 | 6249764 |
| properties of herpes simplex virus type 1 and type 2 dna polymerase. | herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ... | 1980 | 6250618 |
| experimental infection and the state of viral latency of adult tupaia with herpes simplex virus type 1 and 2 and infection of juvenile tupaia with temperature-sensitive mutants of hsv type 2. | the susceptibility of adult tupaia belangeri to infection with herpes simplex virus (hvs) was investigated. adult animals were inoculated intraperitoneally with hsv type 1 or 2. with the exception of hsv-2, strain hg-52, 10(5)--10(6) pfu of all hsv strains caused lethal infection irrespective of the age of the animals. infections hsv was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. the dna of the recovered viruses was compared to the dna ... | 1980 | 6251787 |
| reduction of cell surface fibronectin (lets protein) correlates with tumorigenicity of hamster fibroblasts transformed by herpes simplex virus type2. | a series of herpes simplex virus type 2 (hsv-2)-transformed hamster cells has been examined using the technique of lactoperoxidase-catalyzed cell surface protein iodination. the amount of cell surface fibronectin (lets protein) was reduced after hsv-2 transformation, and this reduction correlated with increased tumor-forming ability. | 1980 | 6244242 |
| resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci. | acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ... | 1980 | 6246532 |
| elution of herpes simplex virus-specific cytotoxic antibodies from squamous cell carcinoma of uterine cervix. | there is evidence that carcinoma of the cervix uteri is associated with infection with herpes simplex virus type 2 (hsv-2), and although indirect evidence suggests an aetiological relationship, this remains controversial. one facet of the problem is that complement-dependent cytotoxic antibodies is hsv-2-infected cells have been undetectable or found only in low titre in sera from patients with progressive cervical cancer whereas higher titres have been found where cervical lesions are less adva ... | 1980 | 6250079 |
| immunity to herpes simplex virus type 2: cell-mediated immunity in latently infected guinea pigs. | cell-mediated (cmi) and humoral immunity to herpes simplex virus type 2 (hsv-2) were evaluated in infected strain 13/n guinea pigs with (45%) and without a history of recurrent herpetic disease. virus was isolated by cocultivation from active herpetic lesions (9 of 10) as well as from the footpads (17 of 38), sacral ganglia (7 of 21), and sciatic nerves (1 of 21) of asymptomatic animals. viral isolates grew in cells of human origin and were neutralized by hyperimmune anti hsv-2 sera. humoral imm ... | 1980 | 6254886 |
| comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2. | we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ... | 1980 | 6255183 |
| an evaluation of herpes simplex virus antigenic markers in the study of established and developing cervical neoplasia. | previous reports from our laboratory have shown that antiserum to "pure" ag-e, a type-common hsv antigen, specifically stains atypical cervical cells in indirect immunofluorescence. these observations have been confirmed and extended. antisera were prepared against the two protein components of pure ag-e, designated icp 12 (m. w. = 140,000) and icp 14 (m. w. = 130,000), and were purified to radiochemical homogeneity by sds-acrylamide gel electrophoresis. the antisera reacted as well as antiserum ... | 1980 | 6255843 |
| depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced tumors. | total hemolytic complement (ch50) levels were compared in sera from normal hamsters and hamsters bearing tumors derived from herpes simplex virus type 2-transformed cells ch50 in normal sera ranged from 160 to 212 while ch50 in tumor bearer sera ranged from 82 to 146. preincubation of tumor bearer sera with cell surface proteins (csp) from homologous herpes simplex virus type 2-derived tumor cells resulted in a 66% depletion of ch50 whereas preincubation with heterologous herpes simplex virus ty ... | 1980 | 6258785 |
| expression of type-common envelope antigens by herpes simplex virus type 2-transformed hamster cells. | at least ten polypeptides were detected on the surface of the herpes simplex virus type 1 (hsv-1) virion. two of these polypeptides, having molecular weights of 30,000 and 33,000, were identified in two herpes simplex virus type 2-transformed cell lines (333-8-9 and 333-2-29) by immunoprecipitation and sds-page. the same two virion polypeptides previously were shown to be present in an hsv-1-transformed cell line (14-012-8-1, t-10). these observations suggest that genetic information coding for ... | 1980 | 6259083 |
| cloning of herpes simplex virus 2 dna fragments in a plasmid vector. | dna isolated from virions of herpes simplex type 2 (hsv-2) strain 333 was digested with various restriction enzymes and joined to the ek2 plasmid vector pbr322. the viral dna sequences present in the hybrids were analyzed by restriction enzyme mapping and hybridization to fragments of hsv-2 dna. the collection of recombinant molecules represents approx. 75% of the hsv-2-genome. in most cases, the structure of the recombinants seemed identical to the organization of authentic fragments of hsv-2 d ... | 1980 | 6260572 |
| double infection of balb/c mice with rauscher murine leukemia and herpes simplex virus type 2. | when balb/c mice were first infected with rauscher murine leukemia virus (mulv-r) and then superinfected with herpes simplex virus type 2 (hsv-2) the inhibition of the evolution of splenomegaly was observed. the effect did not depend on the route of hsv-2 administration. experiments in which potent anti-interferon serum was administered to double infected mice suggested that the antagonism between mulv-r and hsv-2 was not mediated by endogenous interferon, hsv-2 was found to replicate in the lps ... | 1980 | 6255890 |
| "in vivo" and "in vitro" effects of a bacterial extract on herpes simplex virus. | the present studies were designed to evaluate the effects of substance or substances extracted from escherichia coli on herpes simplex virus. the "in vivo" assays show that bacterial extract introduced i.p. in mice simultaneously with hsv2 brought about 100% of survival, but the inoculation of crude extract after virus challenge brought about complete mortality of mice. "in vitro" assays show that the crude extract reduced significantly the numbers of pfu; better results were obtained when the c ... | 1980 | 6255968 |
| the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication. | both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed. | 1980 | 6255077 |
| quantification of plasminogen activator activity associated with herpesvirus-transformed cells. | herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ... | 1980 | 6255081 |
| localization of the thymidine kinase gene of herpes simplex virus type 2 (333). | the thymidine kinase (tk) gene of herpes simplex virus type 2 (hsv-2) has been identified on purified restriction endonuclease fragments of hsv-2 dna. these fragments were localized on the physical map of hsv-2 dna. a detailed map of the hsv-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). mouse tk- cells that were biochemically tr ... | 1980 | 6263824 |
| effect of corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in balb/c mice. | treatment of 4- to 6-week-old, 18- to 22-g male balb/c mice with 0.6 mg of corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (hsv-2). optimal protection occurred when c. granulosum was injected 1 week before hsv-2 infection. c. granulosum-induced resistance to hsv-2 lasted up to 4.5 weeks. studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated im ... | 1980 | 6262241 |
| interaction of herpesvirus with spleen cell subpopulations comparison of a neurotropic and a lymphotropic virus. | we studied the interaction of a neurotropic herpesvirus, herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2), and a lymphotropic herpesvirus, guinea pig herpes-like virus (hlv), with guinea pig spleen cells. both hsv-1 and hsv-2 and hlv can attach to and penetrate into b- or t-enriched cells. less than 1.4% of the total b- or t-enriched cell populations were susceptible to infection by hlv and to some degree to hsv-1 or hsv-2 as determined by infectious center assays. after specific antiserum ... | 1980 | 6262239 |
| immunity to herpes simplex virus type 2 (hsv-2). i. development of virus-specific lymphoproliferative and leukocyte migration inhibition factor responses in hsv-2-infected guinea pigs. | 1980 | 6258811 | |
| influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine. | the inhibition of herpes simplex virus type 1 (hsv-1) plaque formation by acycloguanosine (acg) was assayed in human fetal lung fibroblasts (hl), cell lines from human cervical carcinoma, rabbit cornea, and human rhabdomyosarcoma, and three green monkey kidney cell lines. the acg concentration giving 50% plaque reduction (pr50) of hsv-1 was lowest in hl. in two green monkey kidney cell lines, hsv-1 plaque formation was relatively insensitive to acg, with pr50 of 25 and 60 mum, respectively. in t ... | 1980 | 6265398 |
| experimental studies on genital herpetic infection in mice. | female icr mice were infected with hsv-1 and hsv-2 by inserting a cotton pellet soaked in viral solution (10(7-8) pfu/m1) into the vagina. the appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. these histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. results of fluorescent antibody studies on the appearance of vir ... | 1980 | 6266394 |
| new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea and tannic acid ointment]. part ii: female patients. | clinical treatment of 147 female patients suffering with genital herpes simplex virus were conducted in two phases. the purpose of phase i was to note the efficacy of treatment with ultrasound and herpigon ointment (zinc, urea, and tannic acid) in relieving pain and shortening the length of time involved in healing. results indicated that the combined treatment significantly reduced pain and length of healing period. phase ii involved a 2-year follow-up of patients with primary and recurrent inf ... | 1980 | 6931874 |
| bilateral necrotizing retinitis complicating fatal encephalitis probably due to herpes simplex virus type 2. | the clinical history, autopsy findings and ocular pathology of a case of localized neonatal herpes simplex virus (hsv) type 2 infection is reported in which the main brunt of the infection fell on the cerebral hemispheres and the peripheral retina. hsv type 2 was isolated from skin vesicles which developed on the trunk and arms and in the mouth 10 days after birth. there was no visceral involvement. although the infant was probably infected at some time during birth, hsv could not be isolated fr ... | 1980 | 7454228 |
| analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus. | peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. in contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. the effect was age dependent. macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for ... | 1980 | 7399667 |
| topical therapeutic efficacy of 9-(2-hydroxyethoxymethyl)guanine and 5-iodo-5'-amino-2',5'-dideoxyuridine on oral infection with herpes simplex virus in mice. | the therapeutic efficacy of two new antiviral agents, 5-iodo-5'-amino-2', 5'-dideoxyuridine (aidurd) and 9-(2-hydroxyethoxymethyl)guanine (acv), in the model of mouse lip inoculated with herpes simplex virus type 2 is reported. the effects on development of clinical lesions, viral replication in the inoculated lips, and establishment of latent viral infection in the trigeminal ganglia were observed. the earlier the treatment with aidurd and acv was initiated after inoculation, the better was the ... | 1980 | 6966305 |
| relationship of antibody to outcome in neonatal herpes simplex virus infections. | neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ... | 1980 | 7216423 |
| estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method. | a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ... | 1980 | 7219207 |
| properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype. | we have isolated cells with a thymidine kinase-negative (tk(-)) phenotype from cells which carry the herpes simplex virus type 2 tk gene by selection in 5-bromodeoxyuridine or 9-(2-hydroxyethoxymethyl)guanine. both selection routines generated revertants with a frequency of 10(-3) to 10(-4), and resistance to either compound conferred simultaneous resistance to the other. tk(-) revertants fell into three classes: (i) cells that arose by deletion of all virus sequences, (ii) cells that had lost t ... | 1980 | 16789205 |
| effect of herpes simplex virus type 2 and friend erythroleukemia virus infection of ige antibody responses to ascaris antigen in mice. | the ige response to ascaris antigen, as measured by passive cutaneous anaphylactic reactions, was suppressed in mice infected with either herpes simplex virus type 2 (hsv-2) or friend leukemia virus (fv). fv was not suppressive unless infection preceded immunization whereas hsv-2 infection was immunosuppressive before or after immunization. however, the suppression by hsv-2 was relatively short-lived. | 1981 | 7228435 |
| in-vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains. | intra-peritoneal (i.p.) infection of mice with herpes simplex virus type 2 (hsv 2) attracted macrophages into the peritoneum. macrophages from moderately and highly hsv 2 resistant mouse strains expressed elevated phagocytosis activity 24 hours after injection. stimulation of phagocytosis in low resistant strains was generally less effective or absent. this was, in some experiments, due to the fact that macrophages were already highly activated before the experimental infection. i.p. infection a ... | 1981 | 7319542 |
| viruses and gynecologic cancers: herpesvirus protein (icp 10/ag-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity. | the studies associating infections by herpes simplex virus type 2 (hsv-2) with carcinoma of the human uterine cervix are reviewed within the context of three possible interpretations. extensive seroepidemiologic evidence indicates that the virus does not grow preferentially in neoplastic tissue, nor is the association of hsv-2 with cervical carcinoma a reflection of their independent relationship to promiscuity. while a number of infectious agents, including other viruses, are associated with ce ... | 1981 | 7023662 |
| [studies on the placental damage and iugr caused by intravaginally inoculated herpes simplex virus type-2 (hsv-2) during pregnancy (author's transl)]. | it has been recognized that intrauterine growth retardation (iugr) could be caused by viral infection during pregnancy. we have experimentally attempted the intravaginal inoculation of hsv-2 (uw-268) 1.2-2.0 x 10(6) plaque forming unit in the pregnant rat. hsv-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. the invading route of the virus was investigate ... | 1981 | 7234355 |
| herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection. | women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ... | 1981 | 6941038 |
| antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin infection. | win 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ... | 1981 | 7247370 |
| herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association. | sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ... | 1981 | 7284955 |
| mechanism of degradation of duplex dna by the dnase induced by herpes simplex virus. | reaction intermediates formed during the degradation of linear pm2, t5, and lambda dna by herpes simplex virus (hsv) dnase have been examined by agarose gel electrophoresis. digestion of t5 dna by hsv type 2 (hsv-2) dnase in the presence of mn(2+) (endonuclease only) gave rise to 6 major and 12 minor fragments. some of the fragments produced correspond to those observed after cleavage of t5 dna by the single-strand-specific s1 nuclease, indicating that the hsv dnase rapidly cleaves opposite a ni ... | 1981 | 6264148 |
| [new antigen induced by herpes simplex virus in human tumors]. | the study showed the new antigen induced by herpes simplex virus type 2 (hsv-2) to be present not only in cancer tumors of the cervix and corpus uteri, and ovaries but also in malignant tumors of the mammary glands, kidneys, urinary bladder, as well as in tissues of fibrous-cystic mastopathy and fibroadenoma of the mammary glands. in malignant tumors of the cervix, however, the hsv-2-induced antigen was found more frequently and had a higher serological activity than in other tumors. in contrast ... | 1981 | 6278776 |
| experimental studies of acute and recurrent herpes simplex virus infections in the murine heart and dorsal root ganglia. | the multiplication of hsv-1 and hsv-2 strains in the heart and the corresponding dorsal root ganglia (drg) was examined in experimentally infected mice. infectious hsv-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the drg. both the heart and drg yielded infectious hsv-2 from the fourth to the twenty-first day after inoculation. the hsv-2, but not the hsv-1, induced recrudescent disease in chronically infected mice up to 18 months after i ... | 1981 | 6267190 |
| protectivity of herpes simplex virus antigens: studies in mice on the adjuvant effect of piclc and on the dependence of protection on t cell competence. | the efficacy of a herpes simplex virus type 1 (hsv-1) envelope antigen (eag) preparation against hsv infection was studied in t cell competent and t cell deficient mice. immuno-competent mice were successfully protected against herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a hsv-1 envelope antigen (hsv-1 eag) preparation. since hsv-1 eag was considerably less effective than the who ... | 1981 | 6268955 |
| aminoacyl fucosides as possible biochemical markers at tumorigenic and metastatic potential in herpes simplex virus type 2-transformed rat cells. | two classes of aminoacyl fucosides termed fl3 and fl4 were studied as possible markers of tumorigenic and metastatic potential in herpes simplex virus type 2 transformed rat cells. in the present study, clonal cell lines of transformed highly tumorigenic and metastatic (t-ref-g-1.1), weakly tumorigenic and nonmetastatic (t-ref-g-2.1), nontumorigenic (t-ref-g-2.0), and secondary nontransformed rat embryo fibroblast cells were labeled with [3h]fucose, and cell extracts were analyzed for ratio of r ... | 1981 | 6266657 |
| complement-fixing antibody to the ag-4 antigen in herpes simplex virus type 2-infected patients. | sera collected from confirmed herpes simplex virus type 2 (hsv-2) patients were found to be devoid of complement-fixing antibody to the ag-4 antigen at the time of the herpes lesion outbreak in 10 out of 13 cases. however, 1 to 4 weeks after hsv-2 lesion appearance, 28 out of 30 patients acquired complement-fixing antibody to the ag-4 antigen. the sera of these patients contained immunoglobulin m antibody activity and the ability to immunoprecipitate a 160,000-molecular weight early hsv-2 antige ... | 1981 | 6266964 |
| differential immunologic reactivity and processing of glycoproteins ga and gb of herpes simplex virus types 1 and 2 made in vero and hep-2 cells. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) specify five major glycoproteins designated as ga, gb, gc, gd, and ge. previous studies have shown that ga and gb differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. moreover, ga and gb of hsv-1 crossreact in serologic tests with the corresponding glycoproteins of hsv-2. in this paper, we report on the reactivities of ga and gb of hsv-1 and hsv-2 with 24 independently de ... | 1981 | 6272294 |
| latency in vitro using irradiated herpes simplex virus. | human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ... | 1981 | 6267167 |
| physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue. | the physical mapping of six ts mutations of herpes simplex virus type 2 (hsv-2) is presented. the results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 hsv-1/hsv-2 intertypic recombinants. the order of these mutations on the physical map of hsv-2 is unambiguous and correlates almost exactly with the previously published genetic map of timbury & calder (1976). one of the mutants studied (hsv-2 ts12) has apparently two distinct conditionally lethal ts m ... | 1981 | 6267168 |
| roles of polyamines in the replication of animal viruses. | several animal viruses are known to contain significant amounts of polyamines but so far the function of these viral components is poorly understood. in this study the role of polyamines in the replication of two different types of viruses, herpes simplex virus type 2 and semliki forest virus (sfv) has been investigated. purified sfv was found to contain fairly small amounts of polyamines, sufficient to neutralize only about 3% of viral nucleic acid phosphate, i.e., 1/20 of that found in herpes ... | 1981 | 6279979 |
| ocular lesions associated with dissemination of type 2 herpes simplex virus from skin infection in newborn rabbits. | the subcutaneous inoculation of the backs of new zealand white rabbits 17 to 34 hr old with 10(3) 50% tissue culture infection dose (tcid50) of type 2 herpes simplex virus (hsv-2) induced cutaneous lesions within 24 hr, foci of disseminated infection in many organs (including the eye) on day 3 and thereafter, and the death of the animals on day 5 with infection of the central nervous system. infectious hsv-2 could be isolated from the mononuclear cells and plasma of the peripheral blood, indicat ... | 1981 | 6271702 |
| herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication. | in addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ... | 1981 | 6270377 |
| phospholipid synthesis in human embryo fibroblasts infected with herpes simplex virus type 2. | the effect of herpes simplex virus type 2 infection on the synthesis of phospholipids in human embryo fibroblasts was determined at temperatures permissive (35 c) or nonpermissive (42 c) for virus replication. incorporation of [32p]i was decreased by herpes simplex virus type 2 infection after 6 hr, which corresponds to the time of initiation of progeny virus production. no differences were observed in the relative incorporation of [32p]i phospholipid classes. in another series of experiments ce ... | 1981 | 6270493 |
| one functional copy of the long terminal repeat gene specifying the immediate-early polypeptide ie 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus. | the hsv-1/hsv-2 intertypic recombinant bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (irl and trl) and expresses both the immediate-early polypeptide ie 110 of hsv-1 and its functionally equivalent polypeptide ie 118 of hsv-2. the genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. subclone 14 lacked most of the irl sequence, including the region from which part of the mrna ... | 1981 | 6271901 |
| in vitro studies of a co-carcinogenic effect of vaccinia and herpes group viruses. | the results of studies of a co-carcinogenic effect of two human infectious viruses in tissue culture are reported here. viable vaccinia virus actively replicating in the cells of primary balb/c tissue culture and in a number of continuous murine cell lines has been shown to induce in them expression of major structural p30 protein of murine retroviruses. vaccinia virus has been also shown to cause biochemical transformation of murine cells. evidence for the capacity of herpes simplex virus type ... | 1981 | 6270580 |
| antiherpesviral and anticellular effects of 1-beta-d-arabinofuranosyl-e-5-(2-halogenovinyl) uracils. | 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl) uracil (bv-ara-u) and 1-beta-d-arabinofuranosyl-e-5-(2-chlorovinyl)uracil (cv-ara-u) were tested for their anti-herpesviral activity in virus rating method, a plaque reduction method, and a virus yield reduction method, using human embryonic lung fibroblast (hel-f) cells, at a concentration as low as 0.1 microgram/ml, both drugs exerted a marked inhibitory effect on the development of cytopathogenic effect induced by herpes simplex virus type 1 (hsv-1 ... | 1981 | 6269482 |
| herpes virus inactivation by chemical carcinogens: differential inactivation of herpes simplex viruses by 4-nitroquinoline 1-oxide and related compounds. | treatment of stocks of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with the chemical carcinogen 4-nitroquinoline 1-oxide (nqo) resulted in inactivation of virus infectivity at rates which were directly dependent on the concentration of nqo and interval of exposure to nqo. hsv-1 strains were more sensitive than hsv-2 strains to inactivation by nqo, although survival curves of both hsv types were multicomponent. exposure of hsv-2 to a related group of chemicals suggested that the struct ... | 1981 | 6266824 |
| identification of a virus-specific polypeptide associated with a transforming fragment (bglii-n) of herpes simplex virus type 2 dna. | the bglii n fragment of herpes simplex virus type 2 (hsv-2) dna (approximately 0.58 to 0.63 map unit) was examined for encoded products. using plasmid pgz59, which consists of bglii-n cloned in pat153, in conjunction with hybrid arrested translation, mrna selection, and in vitro protein synthesis, we found that the major translated product of this region has an approximate molecular weight of 37,800. by further mapping, coding sequences for this polypeptide were located within the region of bgli ... | 1981 | 6270369 |
| the influence of different modes of immunization on the experimental genital herpes simplex virus infection of mice. | previous investigations, which simulated the usual sequence of the human herpes simplex virus (hsv) infections, had shown that the oral infection of mice with hsv-1 caused only weak protection from genital infection with hsv-2, although the course of infection was attenuated and lethality diminished. this heterologous, heterotopic model was compared with a homologous, heterotopic and a heterologous, homotopic model. the results did not differ very much, although the homologous immunization prote ... | 1981 | 6268958 |
| "the herpesvirus hypothesis"--are koch's postulates satisfied? | 1981 | 6273266 | |
| [studies on the nature of tubular structures as a marker of herpes simplex virus type 2 (author's transl)]. | 1981 | 6273274 | |
| evaluation of the antiherpetic activity of 2'-fluoro-5-iodo-ara-c in rabbit eyes and cell cultures. | 2-fluoro-5-iodo-ara-c (fiac), a new and potent drug, was tested for antiviral activity against several strains of herpes simplex virus (hsv), types 1 and 2. effective dose-50% (ed-50) determinations for fiac ranged from 0.023 to 0.51 mum for hsv-2. fiac-treated cells did not exhibit any toxicity until the drug concentration was increased 2000-fold above the ed-50 level. ocular herpetic keratitis in new zealand white rabbits was treated with 1.0%, 0.1%, 0.01% fiac beginning 3 days after inoculati ... | 1981 | 6273355 |
| [use of a contraceptive suppository as chemoprophylaxis against sexually-transmitted diseases]. | the incidence of sexually transmitted diseases (std), especially gonorrhea, has risen dramatically in the past 2 decades. the population at greatest risk is also at high risk for unwanted pregnancies. since currently employed treatment methods are failing to curb the problem, a search was instituted for an available vaginal contraceptive that would have a prophylactic effect against these diseases. in vitro studies have indicated that a vaginal contraceptive made of phenylmercuric acetate is ... | 1981 | 6277015 |
| sexually transmitted infections and cervical atypia. | epidemiologic studies have long associated cervical cancer and dysplasia with sexual activity. in the past two decades an abundance of biological and epidemiologic data have indicated a relationship between herpes simplex virus type 2 (hsv-2) and these conditions. unfortunately, the strength of the evidence (albeit circumstantial) has led many researchers to focus exclusively on the putative role of hsv-2 as the carcinogen. at the same time, many studies have shown that cervical atypia, if it is ... | 1981 | 6277023 |
| repression and activation of the genome of herpes simplex viruses in human cells. | we have described previously a cell culture system in which the herpes simplex virus (hsv) type 2 (hsv-2) genome is maintained in a repressed form after treatment of infected cells with 1-beta-d-arabinofuranosylcytosine and increase of incubation temperature from 37 degrees c to 39.5 degrees c. infectious hsv-2 production was activated by altering incubation temperature or by superinfecting with human cytomegalovirus. we now report the establishment of an analogous system utilizing hsv type 1 (h ... | 1981 | 6273875 |
| herpes simplex vaccine using cell membrane associated antigen in an animal model. | vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of herpes simplex virus type 2 (hsv-2) infected vero cells with dithiothreitol and formaldehyde. these preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with hsv-2. | 1981 | 6281105 |
| antibody responses in humans to individual proteins of herpes simplex viruses. | sera from 231 women were used to examine their frequency of precipitation of various herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) proteins and to determine if there was a rank order of immune responsiveness of humans to these hsv antigens. radiolabeled viral proteins were reacted with serum and immune complexes isolated with staphylococcal protein a. individual antigens were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. as a group, these sera precipitated ... | 1981 | 6277791 |
| linkage map of cotransfected viral dna sequences in a mouse 3t3 cell line. | a physical map was constructed of a cotransformed 3t3 thymidine kinase (tk) negative cell line revealing a close linkage of the selectable herpes simplex virus type 2 tk gene and cotransfected simian virus 40 dna sequences in high-molecular-weight cellular dna. | 1981 | 6277824 |
| [malignant transformation of newborn hamster cells by herpes simplex virus type 2]. | the oncogenic potentials of hsv-2 inactivated by ultraviolet rays in newborn hamster cell culture were studied. virus-induced morphological and malignant transformation of cells was accompanied by synthesis of virus-specific antigen, changes in morphology, formation of colonies in semi-liquid agar, and tumorigenicity of cells. the animals bearing tumors induced by transformed cells showed humoral and cellular immune responses to the virus-specific antigen. lines of transformed and tumor cells we ... | 1981 | 6278782 |
| [new antigens induced by herpes simplex virus in human tumors]. | tissues of malignant tumors of the genitalia (cervix, uterus body, ovary) contain a specific antigen associated with herpes simplex virus type 2 (hsv-2) identical with the antigen of the infected cells. the virus-induced antigen was detected in tissues of cervical carcinoma in 35% of cases, in 15% of tumors of the corpus uteri and in 13% of ovary tumors. hsv-2 was isolated from pathologically altered cervical carcinoma cells in 2 out of 56 cases examined. these facts indicate the presence of hsv ... | 1981 | 6267821 |
| the development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses. | two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of mrc-5 and vero cells, and herpes simplex type 2 virus. cell growth was similar in both systems giving yields of 14 x 10(5)/cm2 in microcarrier and 18 x 10(5)/cm2 in glass sphere cultures with yields of vero cells being marginally better than mrc-5 cells. virus yields were only slightly lower in these cultures than in small scale stationary cultures ... | 1981 | 6281104 |
| molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection. | in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ... | 1981 | 6275122 |
| microscopic examination of the intracellular fate of infectious herpes simplex virus dna. | the intracellular fate of 3h-labeled exogenous herpes simplex virus type 2 dna following the exposure of recipient cells to selected dna facilitators and the ultrastructural changes which ensued in transfected cultures were examined. in most cases, electron microscopic radioautography demonstrated that exogenous dna reached the nucleus within 1 h postinoculation. at later time periods, enlargement of nuclei and margination of chromatin were noted. cells treated with polyornithine differed signif ... | 1981 | 6276330 |
| a comparison of phosphonoacetic acid and phosphonoformic acid activity in genital herpes simplex virus type 1 and type 2 infections of mice. | the activity of phosphonoacetic acid (paa) and phosphonoformic acid (pfa) against four strains of herpes simplex virus type 1 (hsv-1) and four strains of hsv-2 were compared in tissue culture and in a murine model of genital herpes. in mouse embryo fibroblast cells, both drugs were three-fold more active against the hsv-1 strains than against the hsv-2 strains. in contrast, in the animal model infections, paa appeared to be more active against the hsv-2 strains, while pfa was equally effective a ... | 1981 | 6280607 |
| expression of herpes simplex virus common surface antigens and malignancy by clonal cells of a herpes simplex virus type 2-transformed line. | herpes simplex virus (hsv) type common surface antigens (csa) were examined by indirect immunofluorescence with rabbit antiserum to hsv type 1 in a clonal hamster cell line 155-4 transformed by hsv type 2. the tumor formation was examined in hamsters transplanted with various transformed and tumor cells. the examination of subclones derived from 1554-cell line gave the following results. (1) thirty subclones were isolated and classified into three phenotypes as to csa expression: (i) in csa-pos ... | 1981 | 6281115 |
| antiherpes activity of [e]-5-(1-propenyl)-2'-deoxyuridine and 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil. | 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil has been synthesized, and this compound and [e]-5-(-propenyl)-2'-deoxyuridine have been tested for inhibition of herpes virus multiplication. only [e]-5-(1-propenyl)-2'-deoxyuridine was found to be an active inhibitor reducing by 50% the plaque formation of herpes simplex virus type 1 (hsv-1) at about 1 mum. a comparison to the bromovinyl derivatives showed the following order of descending activity; [e]-5-(2-bromovinyl)-2'-deoxyuridine greater than ... | 1981 | 6280606 |
| acyclovir therapy of neonatal herpes simplex virus type 2 infections in rabbits. | new zealand white rabbits less than 30 h old were inoculated subcutaneously with 10(3) 50% tissue culture infectious doses of type 2 herpes simplex virus. the animals were randomly assigned to a treatment schedule of daily intraperitoneal injections of acyclovir, beginning on the day of virus inoculation for 6 or 12 days, on post-inoculation day 1 for 6 days, or on post-inoculation day 2 for 6 days. the acyclovir was given in doses of 50 mg/kg of body weight per day. similarly infected animals r ... | 1981 | 6282195 |
| cell-mediated immunity to herpes simplex virus: recognition of type-specific and type-common surface antigens by cytotoxic t cell populations. | in this communication, we examine the specificity of anti-herpes simplex virus (hsv) cytotoxic t lymphocytes (ctl). serological studies of the two related hsv serotypes (hsv-1 and hsv-2) have revealed both type-specific and cross-reactive antigenic determinants in the viral envelope and on the surface of infected cells. by analysis of cytotoxicity of ctl, generated in vitro by restimulation of splenocytes from mice primed with one or the other hsv serotype, the recognition of both type-specific ... | 1981 | 6277790 |
| pathogenesis of herpes simplex virus infections in guinea pigs. | the pathogenesis of herpes simplex virus types 1 and 2 has been studied in guinea pigs after inoculation by various routes (subcutaneous and intradermal infection in footpads and vaginal infection). clinical observations as well as virus isolation studies are reported. herpes simplex virus type 2 infection by all three routes of inoculation led to acute primary and recurrent lesions. virus persisted in the nervous system, particularly in sensory ganglia, and locally at the site of inoculation. h ... | 1981 | 6277787 |
| monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein icp 4. | monoclonal antibodies were prepared against herpes simplex virus type 1 (strain 14012) by two immunization procedures. procedure a utilized infectious virus propagated in mouse cells, and procedure b utilized mouse cells infected with herpes simplex virus in the presence of cycloheximide and harvested 1 h after removal of the inhibitor. a total of 52 monoclonal antibodies were obtained against 10 herpes simplex virus proteins, including four glycosylated proteins (a 110,000-molecular-weight prot ... | 1981 | 6277788 |
| specific lymphocyte blastogenic responses in children with cytomegalovirus and herpes simplex virus infections acquired early in infancy. | cell-mediated immune responses in 27 infants and children with cytomegalovirus (cmv) infection acquired between birth and 1 year of age were compared with responses in 13 children who had neonatal herpes simplex virus (hsv) infection. infection was asymptomatic in 25 of 27 cmv-infected children; the 13 patients with hsv infection were all ill as newborns. the median age when studied was 46 months for children infected with cmv and 24 months for those infected with hsv. we measured lymphocyte tra ... | 1981 | 6271679 |
| consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus. | electron microscopic examination showed microtubular structures in fl cells infected with all 18 strains of hsv-2 examined, but not in cells infected with 9 strains of hsv-1. these structures were also detected in other cultured cells (vero and earle's l cells) infected with hsv-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (ddd strain) infected with this type of virus. thus the microtubules were consistently detected in ... | 1981 | 6272691 |