TitleAbstractYear(sorted ascending)
analysis of viral dna sequences in hamster cells transformed by herpes simplex virus type 2.herpes simplex virus type 2 (hsv-2) dna was treated with four restriction endonucleases (ecori, hinciii, bgl ii, and xba i) and eight fragments were purified and labeled with 32p in vitro. the kinetics of renaturation of each of the fragments was measured in the presence of dna extracted from 333-8-9, a hamster cell line transformed by uv light-inactivated hsv-2 strain 333, and from a series of cloned derivatives and their tumor lines. all of the lines examined contained a partial set of viral s ...19806244590
cytomegalovirus and dual infection in four unrelated infants without underlying immunodeficiency, dual infections with cytomegalovirus (cmv) and another microorganism developed. the patients included the following: (1) a 3-month-old girl with congenital cmv and perinatal cutaneous herpes simplex virus; (2) a 5-week-old girl with cmv and pneumocystis carinii; (3) a 12-week-old girl with cmv and haemophilus influenzae meningitis; and, (4) a 2 1/2-month-old girl with cmv and escherichia coli meningitis. in all four cases, the patien ...19806244804
some characteristics of an early protein (icp 22) synthesized in cells infected with herpes simplex vero cells incubated at 40 degrees c or treated with azetidine at 37 degrees c, synthesis of a polypeptide ('c') of apparent mol. wt. 66000 was stimulated. it was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. in cells infected with herpes simplex virus type 1 (hsv-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide c, was suppressed and infected cell polypeptides icp 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and ...19806245173
identification of the herpes simplex virus dna sequences present in six herpes simplex virus thymidine kinase-transformed mouse cell lines.we have used a novel filter hybridization approach to detect and map the herpes simplex virus (hsv) dna sequences which are present in four hsv thymidine kinase (hsvtk+)-transformed cell lines which were derived by exposure of thymidine kinase negative (tk-) mouse cells to uv light-irradiated hsv type 2 (hsv-2). in addition, we have mapped the hsv-1 dna sequences which are present in two hsv-1tk+-transformed cell lines produced by transfection of tk- mouse cells with sheared hsv-1 dna. the resul ...19806245232
location and cloning of the herpes simplex virus type 2 thymidine kinase gene.the herpes simplex virus type 2 thymidine kinase gene has been mapped to a position colinear with the herpes simplex virus type 1 thymidine kinase gene and cloned within a 4.0-kilobase fragment in pbr 322.19806245273
specific viral antigens in rat cells transformed by herpes simplex virus type 2 and in rat tumors induced by inoculation of transformed cells.a rat cell line, re1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. a high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus thymidine kinase, and there is a correlation between enzyme activity in a tumor and antibody in "tumor s ...19806245808
experimental type 2 herpes simplex ophthalmitis in the newborn animal model for the study of type 2 herpes simplex virus (hsv2) ophthalmitis is described. wistar rats were inoculated intracerebrally with hsv2 at 0, 5, 10, 15, 20, or 30 days of age. eyes and brain from all animals, whether they survived or succumbed with encephalitis, were collected for microscopic and virologic studies. up to 20% or more of the hsv2-inoculated rats had lesions in the cornea, uveal tract, and/or retina. herpetic keratiis occurred in a few animals while the eyelids were st ...19806246021
tubular structures in the cervix of mice experimentally infected with herpes simplex virus type 2.experimental infection of the c3h/n mouse genital tract was demonstrated after intravaginal inoculation with herpes simplex virus type 2 (hsv-2). about 75% of the infected animals died by day 7, and 75% of the surviving animals had severe vaginitis or neurological signs on day 7. titers of the virus recovered from vaginal secretions of infected animals reached a maximum on day 2 and gradually decreased until day 7. on the other hand, under the electron microscope, virus particles and tubular str ...19806246195
resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci.acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ...19806246532
tumorigenic transformation induced by a specific fragment of dna from herpes simplex virus type 2.transfection of syrian hamster embryo cells with limit digests of bgl ii-, hpa i-, or bgl ii/hpa i-cleaved dna from herpes simplex virus type 2 (strain s-1) but not with salmon sperm dna resulted in the appearance of refractile, morphologically altered cells at a frequency of 10(-5)/0.005 microgram of viral dna within two to four passages. transformed lines manifested reduced serum requirement and anchorage-independent growth and were tumorigenic in newborn hamsters. they expressed icp10, a vira ...19806246534
herpes simplex virus infection: problems and prospects as perceived by a peripatetic pediatrician.the multivaried aspects of the herpes simplex viruses (hsv) types 1 and 2 and the infections they produce are discussed. points emphasized are: (1) the need for considering these (and other viruses) from an evolutionary perspective; (2) the necessity of disseminating current methods for virus identification; (3) the great progress in molecular-virological aspects and in the genetics of the virus which provide new tools for epidemiological and immunological studies and define more convincingly th ...19806246686
comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains.this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ...19806246854
collagenase levels in a new model of experimental herpetic interstitial keratitis.a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ...19806246865
macrophage extrinsic antiviral activity during herpes simplex virus infection.peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ...19806247426
comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice.the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ...19806247441
use of [125i]deoxycytidine to detect herpes simplex virus-specific thymidine kinase in tissues of latently infected guinea pigs.the footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (hsv-2) in both hind footpads. these tissues, shown by our previous study to harbor latent hsv, were dispersed into single cells. the presence of virus-specific thymidine kinase (tk) in these cells was assayed by the uptake and phosphorylation of [125i]deoxycytidine in culture. [125i]deoxycytidine was shown to be a specific ...19806247502
genetic retrieval of viral genome sequences from herpes simplex virus transformed cells.oncogenic transformation of cultured cells by inactivated herpes simplex virus (hsv) types 1 and 2 has been demonstrated. expression of hsv information in these transformed cells has been shown by immunofluorescence studies, detection of hsv neutralizing antibody in sera from tumour-bearing animals and by hybridization of hsv-specific rna. molecular hybridization studies of dna from hsv-2 transformed hamster cells have detected up to 40% of the hsv genome present in several copies. complementati ...19806247659
[isolation of an herpes simplex virus type 2 from a human cervical cancer by means of a cell cocultivation]. 19806247704
[cellular immune response detected in blast transformation test with lymphocytes from rabbits immunized with nucleocapsid of herpes simplex type 2 virus].a preparation of herpes simplex virus type 2 (hsv-2) nucleocapsid (nc) was obtained after treatment of intracellular virus with detergents followed by centrifugation in linear sucrose density gradient. electron microscopic analysis revealed polymorphism of hsv-2 nc population. three main types of capsid structures were found: empty capsids, nc containing various amounts of nucleoid material, and nc with complete nucleoid. morphological heterogeneity correlated with heterogenous sedimentation pro ...19806247843
oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition.5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ...19806248643
immunoprecipitation of polypeptides from hamster embryo cells transformed by herpes simplex virus type 2. 19806249032
combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2.the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ...19806249191
differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells.fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ...19806249749
cervical carcinoma: detection of herpes simplex virus rna in cells undergoing neoplastic change.3h-hsv-2 dna has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. rna complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer.19806249760
ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2.the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ...19806249764
elution of herpes simplex virus-specific cytotoxic antibodies from squamous cell carcinoma of uterine cervix.there is evidence that carcinoma of the cervix uteri is associated with infection with herpes simplex virus type 2 (hsv-2), and although indirect evidence suggests an aetiological relationship, this remains controversial. one facet of the problem is that complement-dependent cytotoxic antibodies is hsv-2-infected cells have been undetectable or found only in low titre in sera from patients with progressive cervical cancer whereas higher titres have been found where cervical lesions are less adva ...19806250079
[effect of the physiological status of culture on herpes simplex virus type 2 infection of synchronized cells bnk-21].infecting of synchronized cells-bnk-21 by herpes simplex virus type 2 in the s period resulted in a secondary rise of the labeled cells index and shortened time of the cell cycle as a whole. while infecting of the cells during the s period some morphological transformation was observed. the infecting of the cells in the periods m, g1, g2 revealed no essential changes in cell dna synthesis and failed to result in any cell transformation.19806250284
properties of herpes simplex virus type 1 and type 2 dna polymerase.herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ...19806250618
experimental infection and the state of viral latency of adult tupaia with herpes simplex virus type 1 and 2 and infection of juvenile tupaia with temperature-sensitive mutants of hsv type 2.the susceptibility of adult tupaia belangeri to infection with herpes simplex virus (hvs) was investigated. adult animals were inoculated intraperitoneally with hsv type 1 or 2. with the exception of hsv-2, strain hg-52, 10(5)--10(6) pfu of all hsv strains caused lethal infection irrespective of the age of the animals. infections hsv was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. the dna of the recovered viruses was compared to the dna ...19806251787
herpes simplex virus phosphoproteins. ii. characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion.the protein kinase associated with purified herpes simplex virus 1 and 2 virions partitioned with the capsid-tegument structures and was not solubilized by non-ionic detergents and low, non-inhibitory concentrations of urea. the enzyme required mg2+ or mn2+ and utilized atp or gtp. the activity was enhanced by non-ionic detergents and by na+ even in the presence of high concentrations of of mg2+, but not by cyclic nucleotides. the enzyme associated with capsid-tegument structures phosphorylated ...19806252339
temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mrna's.nuclear and cytoplasmic virus rnas, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus dna fragments generated by bam hi and eco ri restriction endonucleases. polyadenylated immediate early mrnas were separated on denaturing gels containing ch3hgoh giving three virus-specific mrna bands of estimate ...19806253886
responses of mouse spleen morphology to the growth of subcutaneously injected virally transformed cells.the changes in the fractional volume of six structural components in the spleens of balb/c mice injected with herpes simplex virus type 2-transformed cells (h238 tumor cells) were quantitated during progressive tumor growth. spleen stereology was performed at three time intervals during the early stages of tumor development. the results revealed that the volume of the compact myeloid tissue and reaction center of lymphoid nodules increased about four- to five-fold from 10 to 33 days after h238 t ...19806254396
comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections.indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ...19806254476
reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls.serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ...19806254649
pathogenicity of wild-type and temperature-sensitive mutants of herpes simplex virus type 2 in guinea pigs.the pathogenicity of herpes simplex virus type 2 strain 186, the wild-type (wt) strain, and four temperature-sensitive (ts) mutants was studied after genital inoculation of female guinea pigs. infection with the wt virus was generally severe, with extensive skin lesions in 89% and mortality in 37% of inoculated animals. guinea pigs inoculated with ts mutants manifest remarkably mild disease, with lesions occurring in only 16% of the guinea pits and a mortality rate of 7%. wt virus was recovered ...19806254877
immunity to herpes simplex virus type 2: cell-mediated immunity in latently infected guinea pigs.cell-mediated (cmi) and humoral immunity to herpes simplex virus type 2 (hsv-2) were evaluated in infected strain 13/n guinea pigs with (45%) and without a history of recurrent herpetic disease. virus was isolated by cocultivation from active herpetic lesions (9 of 10) as well as from the footpads (17 of 38), sacral ganglia (7 of 21), and sciatic nerves (1 of 21) of asymptomatic animals. viral isolates grew in cells of human origin and were neutralized by hyperimmune anti hsv-2 sera. humoral imm ...19806254886
presence of herpes simplex virus type 2 (hsv-2) antibodies and antigens in patients with cervical carcinoma in thailand. 19806255050
the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication.both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed.19806255077
quantification of plasminogen activator activity associated with herpesvirus-transformed cells.herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ...19806255081
comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2.we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ...19806255183
an evaluation of herpes simplex virus antigenic markers in the study of established and developing cervical neoplasia.previous reports from our laboratory have shown that antiserum to "pure" ag-e, a type-common hsv antigen, specifically stains atypical cervical cells in indirect immunofluorescence. these observations have been confirmed and extended. antisera were prepared against the two protein components of pure ag-e, designated icp 12 (m. w. = 140,000) and icp 14 (m. w. = 130,000), and were purified to radiochemical homogeneity by sds-acrylamide gel electrophoresis. the antisera reacted as well as antiserum ...19806255843
double infection of balb/c mice with rauscher murine leukemia and herpes simplex virus type 2.when balb/c mice were first infected with rauscher murine leukemia virus (mulv-r) and then superinfected with herpes simplex virus type 2 (hsv-2) the inhibition of the evolution of splenomegaly was observed. the effect did not depend on the route of hsv-2 administration. experiments in which potent anti-interferon serum was administered to double infected mice suggested that the antagonism between mulv-r and hsv-2 was not mediated by endogenous interferon, hsv-2 was found to replicate in the lps ...19806255890
"in vivo" and "in vitro" effects of a bacterial extract on herpes simplex virus.the present studies were designed to evaluate the effects of substance or substances extracted from escherichia coli on herpes simplex virus. the "in vivo" assays show that bacterial extract introduced i.p. in mice simultaneously with hsv2 brought about 100% of survival, but the inoculation of crude extract after virus challenge brought about complete mortality of mice. "in vitro" assays show that the crude extract reduced significantly the numbers of pfu; better results were obtained when the c ...19806255968
experimental studies on genital herpetic infection in mice.female icr mice were infected with hsv-1 and hsv-2 by inserting a cotton pellet soaked in viral solution (10(7-8) pfu/m1) into the vagina. the appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. these histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. results of fluorescent antibody studies on the appearance of vir ...19806266394
new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea and tannic acid ointment]. part ii: female patients.clinical treatment of 147 female patients suffering with genital herpes simplex virus were conducted in two phases. the purpose of phase i was to note the efficacy of treatment with ultrasound and herpigon ointment (zinc, urea, and tannic acid) in relieving pain and shortening the length of time involved in healing. results indicated that the combined treatment significantly reduced pain and length of healing period. phase ii involved a 2-year follow-up of patients with primary and recurrent inf ...19806931874
topical therapeutic efficacy of 9-(2-hydroxyethoxymethyl)guanine and 5-iodo-5'-amino-2',5'-dideoxyuridine on oral infection with herpes simplex virus in mice.the therapeutic efficacy of two new antiviral agents, 5-iodo-5'-amino-2', 5'-dideoxyuridine (aidurd) and 9-(2-hydroxyethoxymethyl)guanine (acv), in the model of mouse lip inoculated with herpes simplex virus type 2 is reported. the effects on development of clinical lesions, viral replication in the inoculated lips, and establishment of latent viral infection in the trigeminal ganglia were observed. the earlier the treatment with aidurd and acv was initiated after inoculation, the better was the ...19806966305
relationship of antibody to outcome in neonatal herpes simplex virus infections.neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ...19807216423
estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method.a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ...19807219207
analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus.peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. in contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. the effect was age dependent. macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for ...19807399667
bilateral necrotizing retinitis complicating fatal encephalitis probably due to herpes simplex virus type 2.the clinical history, autopsy findings and ocular pathology of a case of localized neonatal herpes simplex virus (hsv) type 2 infection is reported in which the main brunt of the infection fell on the cerebral hemispheres and the peripheral retina. hsv type 2 was isolated from skin vesicles which developed on the trunk and arms and in the mouth 10 days after birth. there was no visceral involvement. although the infant was probably infected at some time during birth, hsv could not be isolated fr ...19807454228
properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.we have isolated cells with a thymidine kinase-negative (tk(-)) phenotype from cells which carry the herpes simplex virus type 2 tk gene by selection in 5-bromodeoxyuridine or 9-(2-hydroxyethoxymethyl)guanine. both selection routines generated revertants with a frequency of 10(-3) to 10(-4), and resistance to either compound conferred simultaneous resistance to the other. tk(-) revertants fell into three classes: (i) cells that arose by deletion of all virus sequences, (ii) cells that had lost t ...198016789205
effect of herpes simplex virus type 2 and friend erythroleukemia virus infection of ige antibody responses to ascaris antigen in mice.the ige response to ascaris antigen, as measured by passive cutaneous anaphylactic reactions, was suppressed in mice infected with either herpes simplex virus type 2 (hsv-2) or friend leukemia virus (fv). fv was not suppressive unless infection preceded immunization whereas hsv-2 infection was immunosuppressive before or after immunization. however, the suppression by hsv-2 was relatively short-lived.19817228435
[studies on the placental damage and iugr caused by intravaginally inoculated herpes simplex virus type-2 (hsv-2) during pregnancy (author's transl)].it has been recognized that intrauterine growth retardation (iugr) could be caused by viral infection during pregnancy. we have experimentally attempted the intravaginal inoculation of hsv-2 (uw-268) 1.2-2.0 x 10(6) plaque forming unit in the pregnant rat. hsv-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. the invading route of the virus was investigate ...19817234355
antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ...19817247370
herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association.sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ...19817284955
in-vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains.intra-peritoneal (i.p.) infection of mice with herpes simplex virus type 2 (hsv 2) attracted macrophages into the peritoneum. macrophages from moderately and highly hsv 2 resistant mouse strains expressed elevated phagocytosis activity 24 hours after injection. stimulation of phagocytosis in low resistant strains was generally less effective or absent. this was, in some experiments, due to the fact that macrophages were already highly activated before the experimental infection. i.p. infection a ...19817319542
herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection.women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ...19816941038
viruses and gynecologic cancers: herpesvirus protein (icp 10/ag-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity.the studies associating infections by herpes simplex virus type 2 (hsv-2) with carcinoma of the human uterine cervix are reviewed within the context of three possible interpretations. extensive seroepidemiologic evidence indicates that the virus does not grow preferentially in neoplastic tissue, nor is the association of hsv-2 with cervical carcinoma a reflection of their independent relationship to promiscuity. while a number of infectious agents, including other viruses, are associated with ce ...19817023662
roles of polyamines in the replication of animal viruses.several animal viruses are known to contain significant amounts of polyamines but so far the function of these viral components is poorly understood. in this study the role of polyamines in the replication of two different types of viruses, herpes simplex virus type 2 and semliki forest virus (sfv) has been investigated. purified sfv was found to contain fairly small amounts of polyamines, sufficient to neutralize only about 3% of viral nucleic acid phosphate, i.e., 1/20 of that found in herpes ...19816279979
aminoacyl fucosides as possible biochemical markers at tumorigenic and metastatic potential in herpes simplex virus type 2-transformed rat cells.two classes of aminoacyl fucosides termed fl3 and fl4 were studied as possible markers of tumorigenic and metastatic potential in herpes simplex virus type 2 transformed rat cells. in the present study, clonal cell lines of transformed highly tumorigenic and metastatic (t-ref-g-1.1), weakly tumorigenic and nonmetastatic (t-ref-g-2.1), nontumorigenic (t-ref-g-2.0), and secondary nontransformed rat embryo fibroblast cells were labeled with [3h]fucose, and cell extracts were analyzed for ratio of r ...19816266657
herpes virus inactivation by chemical carcinogens: differential inactivation of herpes simplex viruses by 4-nitroquinoline 1-oxide and related compounds.treatment of stocks of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with the chemical carcinogen 4-nitroquinoline 1-oxide (nqo) resulted in inactivation of virus infectivity at rates which were directly dependent on the concentration of nqo and interval of exposure to nqo. hsv-1 strains were more sensitive than hsv-2 strains to inactivation by nqo, although survival curves of both hsv types were multicomponent. exposure of hsv-2 to a related group of chemicals suggested that the struct ...19816266824
complement-fixing antibody to the ag-4 antigen in herpes simplex virus type 2-infected patients.sera collected from confirmed herpes simplex virus type 2 (hsv-2) patients were found to be devoid of complement-fixing antibody to the ag-4 antigen at the time of the herpes lesion outbreak in 10 out of 13 cases. however, 1 to 4 weeks after hsv-2 lesion appearance, 28 out of 30 patients acquired complement-fixing antibody to the ag-4 antigen. the sera of these patients contained immunoglobulin m antibody activity and the ability to immunoprecipitate a 160,000-molecular weight early hsv-2 antige ...19816266964
latency in vitro using irradiated herpes simplex virus.human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ...19816267167
physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue.the physical mapping of six ts mutations of herpes simplex virus type 2 (hsv-2) is presented. the results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 hsv-1/hsv-2 intertypic recombinants. the order of these mutations on the physical map of hsv-2 is unambiguous and correlates almost exactly with the previously published genetic map of timbury & calder (1976). one of the mutants studied (hsv-2 ts12) has apparently two distinct conditionally lethal ts m ...19816267168
experimental studies of acute and recurrent herpes simplex virus infections in the murine heart and dorsal root ganglia.the multiplication of hsv-1 and hsv-2 strains in the heart and the corresponding dorsal root ganglia (drg) was examined in experimentally infected mice. infectious hsv-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the drg. both the heart and drg yielded infectious hsv-2 from the fourth to the twenty-first day after inoculation. the hsv-2, but not the hsv-1, induced recrudescent disease in chronically infected mice up to 18 months after i ...19816267190
[new antigens induced by herpes simplex virus in human tumors].tissues of malignant tumors of the genitalia (cervix, uterus body, ovary) contain a specific antigen associated with herpes simplex virus type 2 (hsv-2) identical with the antigen of the infected cells. the virus-induced antigen was detected in tissues of cervical carcinoma in 35% of cases, in 15% of tumors of the corpus uteri and in 13% of ovary tumors. hsv-2 was isolated from pathologically altered cervical carcinoma cells in 2 out of 56 cases examined. these facts indicate the presence of hsv ...19816267821
differentiation of members of the human herpesviridae family by radioimmunoassay.many individuals who are seronegative for one member of the human herpesviridae family are strongly seropositive for other members. using sera from such individuals, the radioimmunoassay technique demonstrated absence of antigen-antibody cross-reactions between varicella-zoster virus (vzv) and herpes simplex virus (hsv) at levels of less than one part in 1,000. sera containing antibody to both hsv and vzv were absorbed with antigens of one agent without significantly altering the amount of remai ...19816268545
protectivity of herpes simplex virus antigens: studies in mice on the adjuvant effect of piclc and on the dependence of protection on t cell competence.the efficacy of a herpes simplex virus type 1 (hsv-1) envelope antigen (eag) preparation against hsv infection was studied in t cell competent and t cell deficient mice. immuno-competent mice were successfully protected against herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a hsv-1 envelope antigen (hsv-1 eag) preparation. since hsv-1 eag was considerably less effective than the who ...19816268955
the influence of different modes of immunization on the experimental genital herpes simplex virus infection of mice.previous investigations, which simulated the usual sequence of the human herpes simplex virus (hsv) infections, had shown that the oral infection of mice with hsv-1 caused only weak protection from genital infection with hsv-2, although the course of infection was attenuated and lethality diminished. this heterologous, heterotopic model was compared with a homologous, heterotopic and a heterologous, homotopic model. the results did not differ very much, although the homologous immunization prote ...19816268958
antiherpesviral and anticellular effects of 1-beta-d-arabinofuranosyl-e-5-(2-halogenovinyl) uracils.1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl) uracil (bv-ara-u) and 1-beta-d-arabinofuranosyl-e-5-(2-chlorovinyl)uracil (cv-ara-u) were tested for their anti-herpesviral activity in virus rating method, a plaque reduction method, and a virus yield reduction method, using human embryonic lung fibroblast (hel-f) cells, at a concentration as low as 0.1 microgram/ml, both drugs exerted a marked inhibitory effect on the development of cytopathogenic effect induced by herpes simplex virus type 1 (hsv-1 ...19816269482
induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2.a series of studies were performed to evaluate the oncogenic potential of inactivated herpes simplex viruses types 1 (hsv-1) and 2 (hsv-2) in the mouse cervix. hsv-1 or hsv-2 prepared in hep-2 cell cultures and inactivated by exposure to formalin or ultraviolet light was applied to the mouse cervix for periods ranging from 20 to 90 weeks. control mice were exposed for the same period to control fluids. vaginal cytologic preparations from all animals were examined weekly to detect epithelial abno ...19816269724
recovery of mice from herpes simplex virus type 2 hepatitis: adoptive transfer of recovery with immune spleen cells.young balb/c mice inoculated intraperitoneally with herpes simplex virus type 2 develop focal necrotizing hepatitis. after infection, the livers of these mice show increasing virus titers, which reach a maximum on day 3 after infection; this is followed by a dramatic decrease in the amount of virus recovered on days 4 and 5. this decrease in virus content is accompanied by a progressive infiltration of the lesions with mononuclear leukocytes and an apparent resolution of the lesions. adoptive tr ...19816269998
conversion of herpetic lesions to malignancy by ultraviolet exposure and promoter application.many lines of evidence exist associating herpes simplex virus (hsv) with the development of carcinoma, but much of this evidence is anecdotal or associative in nature and does not prove a cause and effect. the purpose of this research was to investigate the oncogenic potential of hsv type 2 (hsv-2) in vivo. a mouse model for lip carcinogenesis was designed to combine hsv-2 infection, u.v. exposure and tetradecanoyl-phorbol-acetate (tpa) application. hsv-2 inoculation on to abraded mouse lips was ...19816270265
nucleotide sequences of the joint between the l and s segments of herpes simplex virus types 1 and 2.the a sequence of herpes simplex virus (hsv) is present as a direct repeat at the genomic termini and also in inverted orientation at the joint between the l and s segments. dna sequences have been determined for the joint regions of the genomes of hsv-1 and hsv-2, and relative to these sequences the genomic termini are in both cases located close to a short direct repeat of 17 to 21 base pairs (bp) at the b-a and a-c junctions. the hsv-1 joint region contains three separate tandem direct reiter ...19816270266
physical mapping of drug resistance mutations defines an active center of the herpes simplex virus dna polymerase enzyme.the genome structures of herpes simplex virus type 1 (hsv-1)/hsv-2 intertypic recombinants have been previously determined by restriction endonuclease analysis, and these recombinants and their parental strains have been employed to demonstrate that mutations within the hsv dna polymerase locus induce an altered hsv dna polymerase activity, exhibiting resistance to three inhibitors of dna polymerase. the viral dna polymerases induced by two recombinants and their parental strains were purified a ...19816270349
nonstructural proteins of herpes simplex virus. ii. major virus-specific dna-binding protein.the major herpes simplex virus type 2 dna-binding infected cell-specific polypeptides 11 and 12 have been purified to homogeneity from extracts of virus-infected cells. monospecific antiserum to the purified protein has been made and used to examine virus temperature-sensitive mutants for defects in the synthesis of the protein and to probe virus dna synthesis in isolated chromatin. the purified protein acted directly on a polydeoxyadenylic acid-polydeoxythymidylic acid helix, reducing its melti ...19816270358
identification of a virus-specific polypeptide associated with a transforming fragment (bglii-n) of herpes simplex virus type 2 dna.the bglii n fragment of herpes simplex virus type 2 (hsv-2) dna (approximately 0.58 to 0.63 map unit) was examined for encoded products. using plasmid pgz59, which consists of bglii-n cloned in pat153, in conjunction with hybrid arrested translation, mrna selection, and in vitro protein synthesis, we found that the major translated product of this region has an approximate molecular weight of 37,800. by further mapping, coding sequences for this polypeptide were located within the region of bgli ...19816270369
herpes simplex virus types 1 and 2 completely help adenovirus-associated virus addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ...19816270377
focus formation and neoplastic transformation by herpes simplex virus type 2 inactivated intracellularly by 5-bromo-2'-deoxyuridine and near uv light.the induction of focus formation in low serum and of neoplastic transformation of syrian hamster embryo cells was examined after the expression of herpes simplex virus type 2 functions. syrian hamster embryo cells infected at a high multiplicity (5 pfu/cell) with 5-bromo-2'-deoxyuridine-labeled herpes simplex virus type 2 (11% substitution of thymidine residues) were exposed to near uv light irradiation at various times postinfection. this procedure specifically inactivated the viral genome, whi ...19816270382
phospholipid synthesis in human embryo fibroblasts infected with herpes simplex virus type 2.the effect of herpes simplex virus type 2 infection on the synthesis of phospholipids in human embryo fibroblasts was determined at temperatures permissive (35 c) or nonpermissive (42 c) for virus replication. incorporation of [32p]i was decreased by herpes simplex virus type 2 infection after 6 hr, which corresponds to the time of initiation of progeny virus production. no differences were observed in the relative incorporation of [32p]i phospholipid classes. in another series of experiments ce ...19816270493
in vitro studies of a co-carcinogenic effect of vaccinia and herpes group viruses.the results of studies of a co-carcinogenic effect of two human infectious viruses in tissue culture are reported here. viable vaccinia virus actively replicating in the cells of primary balb/c tissue culture and in a number of continuous murine cell lines has been shown to induce in them expression of major structural p30 protein of murine retroviruses. vaccinia virus has been also shown to cause biochemical transformation of murine cells. evidence for the capacity of herpes simplex virus type ...19816270580
herpes simplex virus type 2 and cervical cancer. 19816271502
specific lymphocyte blastogenic responses in children with cytomegalovirus and herpes simplex virus infections acquired early in infancy.cell-mediated immune responses in 27 infants and children with cytomegalovirus (cmv) infection acquired between birth and 1 year of age were compared with responses in 13 children who had neonatal herpes simplex virus (hsv) infection. infection was asymptomatic in 25 of 27 cmv-infected children; the 13 patients with hsv infection were all ill as newborns. the median age when studied was 46 months for children infected with cmv and 24 months for those infected with hsv. we measured lymphocyte tra ...19816271679
use of monoclonal antibody directed against herpes simplex virus glycoproteins to protect mice against acute virus-induced neurological disease.monoclonal antibodies hcl and hd1, directed against herpes simplex virus type 1 (hsv-1) glycoproteins gc and gd, respectively, were evaluated for their ability to passively immunize mice against acute virus-induced neurological disease after footpad inoculation with hsv-1 or herpes simplex virus type 2 (hsv-2). control virus-infected mice receiving a single intraperitoneal injection of normal serum died within 7 to 10 days after the spread of virus from footpad to spinal cord and brain. however, ...19816271681
ocular lesions associated with dissemination of type 2 herpes simplex virus from skin infection in newborn rabbits.the subcutaneous inoculation of the backs of new zealand white rabbits 17 to 34 hr old with 10(3) 50% tissue culture infection dose (tcid50) of type 2 herpes simplex virus (hsv-2) induced cutaneous lesions within 24 hr, foci of disseminated infection in many organs (including the eye) on day 3 and thereafter, and the death of the animals on day 5 with infection of the central nervous system. infectious hsv-2 could be isolated from the mononuclear cells and plasma of the peripheral blood, indicat ...19816271702
the detection of dna tumour virus-specific rna sequences in abnormal human cervical biopsies by in situ hybridization.we have used the technique of in situ nucleic acid hybridization and autoradiography of thin frozen sections of human tissue to search for virus rna sequences in human cervical tumours. of cervical biopsies with abnormal cytology, 67% bound herpes simplex virus type 2 (hsv2) 3h-labelled dna probes and 39% bound adenovirus type 2 (ad2) 3h-labelled dna probes, whereas control experiments with phage lambda 3h-labelled dna probes, under the same conditions, bound to only 7% of cases. in contrast, no ...19816271899
one functional copy of the long terminal repeat gene specifying the immediate-early polypeptide ie 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus.the hsv-1/hsv-2 intertypic recombinant bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (irl and trl) and expresses both the immediate-early polypeptide ie 110 of hsv-1 and its functionally equivalent polypeptide ie 118 of hsv-2. the genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. subclone 14 lacked most of the irl sequence, including the region from which part of the mrna ...19816271901
differential immunologic reactivity and processing of glycoproteins ga and gb of herpes simplex virus types 1 and 2 made in vero and hep-2 cells.herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) specify five major glycoproteins designated as ga, gb, gc, gd, and ge. previous studies have shown that ga and gb differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. moreover, ga and gb of hsv-1 crossreact in serologic tests with the corresponding glycoproteins of hsv-2. in this paper, we report on the reactivities of ga and gb of hsv-1 and hsv-2 with 24 independently de ...19816272294
consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus.electron microscopic examination showed microtubular structures in fl cells infected with all 18 strains of hsv-2 examined, but not in cells infected with 9 strains of hsv-1. these structures were also detected in other cultured cells (vero and earle's l cells) infected with hsv-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (ddd strain) infected with this type of virus. thus the microtubules were consistently detected in ...19816272691
"the herpesvirus hypothesis"--are koch's postulates satisfied? 19816273266
[studies on the nature of tubular structures as a marker of herpes simplex virus type 2 (author's transl)]. 19816273274
kaposi's sarcoma. iv. detection of cmv dna, cmv rna and cmna in tumor order to determine whether human cytomegalovirus- (cmv) dna homologous sequences as well as cmv-specific rna(s) and antigen(s) exist in tumor biopsies of kaposi's sarcoma (ks) dna-dna reassociation, rna-dna in situ cytohybridization and anticomplement immunofluorescence test (acif) tests were applied. three of 10 dnas extracted from kaposi sarcoma biopsies contained dna sequences homologous to radioactively labelled human cmv dna probe. the amount of cmv dna in these sarcoma tissues was calcu ...19816273333
evaluation of the antiherpetic activity of 2'-fluoro-5-iodo-ara-c in rabbit eyes and cell cultures.2-fluoro-5-iodo-ara-c (fiac), a new and potent drug, was tested for antiviral activity against several strains of herpes simplex virus (hsv), types 1 and 2. effective dose-50% (ed-50) determinations for fiac ranged from 0.023 to 0.51 mum for hsv-2. fiac-treated cells did not exhibit any toxicity until the drug concentration was increased 2000-fold above the ed-50 level. ocular herpetic keratitis in new zealand white rabbits was treated with 1.0%, 0.1%, 0.01% fiac beginning 3 days after inoculati ...19816273355
differences in neurovirulence among isolates of herpes simplex virus types 1 and 2 in mice using four routes of infection.differences in neurovirulence between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using recent clinical isolates and laboratory-passaged strains in intravaginal, intranasal, intraperitoneal, and intracerebral infections of mice. the hsv-2 isolates caused higher death rates in all four infections. no differences in death rate were observed between recent and passaged isolates of either hsv-1 or hsv-2. after intravaginal inoculation, hsv-1 isolates replicated to higher ...19816273475
repression and activation of the genome of herpes simplex viruses in human cells.we have described previously a cell culture system in which the herpes simplex virus (hsv) type 2 (hsv-2) genome is maintained in a repressed form after treatment of infected cells with 1-beta-d-arabinofuranosylcytosine and increase of incubation temperature from 37 degrees c to 39.5 degrees c. infectious hsv-2 production was activated by altering incubation temperature or by superinfecting with human cytomegalovirus. we now report the establishment of an analogous system utilizing hsv type 1 (h ...19816273875
[possible role of herpes simplex virus type 2 in the etiology of cervical cancer]. 19816274212
inability to rescue viral genes from human cells biochemically transformed by herpes simplex virus type 2 dna. 19816274496
molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ...19816275122
[experimental endophthalmitis induced by herpes simplex virus. 2. intracarotid inoculation (author's transl)]. 19816275689
acyclovir therapy of neonatal herpes simplex virus type 2 infections in zealand white rabbits less than 30 h old were inoculated subcutaneously with 10(3) 50% tissue culture infectious doses of type 2 herpes simplex virus. the animals were randomly assigned to a treatment schedule of daily intraperitoneal injections of acyclovir, beginning on the day of virus inoculation for 6 or 12 days, on post-inoculation day 1 for 6 days, or on post-inoculation day 2 for 6 days. the acyclovir was given in doses of 50 mg/kg of body weight per day. similarly infected animals r ...19816282195
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