Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
location and cloning of the herpes simplex virus type 2 thymidine kinase gene.the herpes simplex virus type 2 thymidine kinase gene has been mapped to a position colinear with the herpes simplex virus type 1 thymidine kinase gene and cloned within a 4.0-kilobase fragment in pbr 322.19806245273
use of [125i]deoxycytidine to detect herpes simplex virus-specific thymidine kinase in tissues of latently infected guinea pigs.the footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (hsv-2) in both hind footpads. these tissues, shown by our previous study to harbor latent hsv, were dispersed into single cells. the presence of virus-specific thymidine kinase (tk) in these cells was assayed by the uptake and phosphorylation of [125i]deoxycytidine in culture. [125i]deoxycytidine was shown to be a specific ...19806247502
comparison of foldback sequences of herpes simplex virus types 1 and 2 dna.the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ...19806243346
[effect of the physiological status of culture on herpes simplex virus type 2 infection of synchronized cells bnk-21].infecting of synchronized cells-bnk-21 by herpes simplex virus type 2 in the s period resulted in a secondary rise of the labeled cells index and shortened time of the cell cycle as a whole. while infecting of the cells during the s period some morphological transformation was observed. the infecting of the cells in the periods m, g1, g2 revealed no essential changes in cell dna synthesis and failed to result in any cell transformation.19806250284
comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections.indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ...19806254476
identification of the herpes simplex virus dna sequences present in six herpes simplex virus thymidine kinase-transformed mouse cell lines.we have used a novel filter hybridization approach to detect and map the herpes simplex virus (hsv) dna sequences which are present in four hsv thymidine kinase (hsvtk+)-transformed cell lines which were derived by exposure of thymidine kinase negative (tk-) mouse cells to uv light-irradiated hsv type 2 (hsv-2). in addition, we have mapped the hsv-1 dna sequences which are present in two hsv-1tk+-transformed cell lines produced by transfection of tk- mouse cells with sheared hsv-1 dna. the resul ...19806245232
presence of herpes simplex virus type 2 (hsv-2) antibodies and antigens in patients with cervical carcinoma in thailand. 19806255050
"in vivo" and "in vitro" effects of a bacterial extract on herpes simplex virus.the present studies were designed to evaluate the effects of substance or substances extracted from escherichia coli on herpes simplex virus. the "in vivo" assays show that bacterial extract introduced i.p. in mice simultaneously with hsv2 brought about 100% of survival, but the inoculation of crude extract after virus challenge brought about complete mortality of mice. "in vitro" assays show that the crude extract reduced significantly the numbers of pfu; better results were obtained when the c ...19806255968
specific viral antigens in rat cells transformed by herpes simplex virus type 2 and in rat tumors induced by inoculation of transformed cells.a rat cell line, re1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. a high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus thymidine kinase, and there is a correlation between enzyme activity in a tumor and antibody in "tumor s ...19806245808
experimental type 2 herpes simplex ophthalmitis in the newborn rat.an animal model for the study of type 2 herpes simplex virus (hsv2) ophthalmitis is described. wistar rats were inoculated intracerebrally with hsv2 at 0, 5, 10, 15, 20, or 30 days of age. eyes and brain from all animals, whether they survived or succumbed with encephalitis, were collected for microscopic and virologic studies. up to 20% or more of the hsv2-inoculated rats had lesions in the cornea, uveal tract, and/or retina. herpetic keratiis occurred in a few animals while the eyelids were st ...19806246021
tubular structures in the cervix of mice experimentally infected with herpes simplex virus type 2.experimental infection of the c3h/n mouse genital tract was demonstrated after intravaginal inoculation with herpes simplex virus type 2 (hsv-2). about 75% of the infected animals died by day 7, and 75% of the surviving animals had severe vaginitis or neurological signs on day 7. titers of the virus recovered from vaginal secretions of infected animals reached a maximum on day 2 and gradually decreased until day 7. on the other hand, under the electron microscope, virus particles and tubular str ...19806246195
immunity to herpes simplex virus type 2 (hsv-2). i. development of virus-specific lymphoproliferative and leukocyte migration inhibition factor responses in hsv-2-infected guinea pigs. 19806258811
resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci.acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ...19806246532
herpes simplex virus infection: problems and prospects as perceived by a peripatetic pediatrician.the multivaried aspects of the herpes simplex viruses (hsv) types 1 and 2 and the infections they produce are discussed. points emphasized are: (1) the need for considering these (and other viruses) from an evolutionary perspective; (2) the necessity of disseminating current methods for virus identification; (3) the great progress in molecular-virological aspects and in the genetics of the virus which provide new tools for epidemiological and immunological studies and define more convincingly th ...19806246686
comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains.this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ...19806246854
macrophage extrinsic antiviral activity during herpes simplex virus infection.peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ...19806247426
comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice.the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ...19806247441
anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy.e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ...19806263181
elution of herpes simplex virus-specific cytotoxic antibodies from squamous cell carcinoma of uterine cervix.there is evidence that carcinoma of the cervix uteri is associated with infection with herpes simplex virus type 2 (hsv-2), and although indirect evidence suggests an aetiological relationship, this remains controversial. one facet of the problem is that complement-dependent cytotoxic antibodies is hsv-2-infected cells have been undetectable or found only in low titre in sera from patients with progressive cervical cancer whereas higher titres have been found where cervical lesions are less adva ...19806250079
properties of herpes simplex virus type 1 and type 2 dna polymerase.herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ...19806250618
herpes simplex virus phosphoproteins. ii. characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion.the protein kinase associated with purified herpes simplex virus 1 and 2 virions partitioned with the capsid-tegument structures and was not solubilized by non-ionic detergents and low, non-inhibitory concentrations of urea. the enzyme required mg2+ or mn2+ and utilized atp or gtp. the activity was enhanced by non-ionic detergents and by na+ even in the presence of high concentrations of of mg2+, but not by cyclic nucleotides. the enzyme associated with capsid-tegument structures phosphorylated ...19806252339
temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mrna's.nuclear and cytoplasmic virus rnas, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus dna fragments generated by bam hi and eco ri restriction endonucleases. polyadenylated immediate early mrnas were separated on denaturing gels containing ch3hgoh giving three virus-specific mrna bands of estimate ...19806253886
responses of mouse spleen morphology to the growth of subcutaneously injected virally transformed cells.the changes in the fractional volume of six structural components in the spleens of balb/c mice injected with herpes simplex virus type 2-transformed cells (h238 tumor cells) were quantitated during progressive tumor growth. spleen stereology was performed at three time intervals during the early stages of tumor development. the results revealed that the volume of the compact myeloid tissue and reaction center of lymphoid nodules increased about four- to five-fold from 10 to 33 days after h238 t ...19806254396
reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls.serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ...19806254649
immunity to herpes simplex virus type 2: cell-mediated immunity in latently infected guinea pigs.cell-mediated (cmi) and humoral immunity to herpes simplex virus type 2 (hsv-2) were evaluated in infected strain 13/n guinea pigs with (45%) and without a history of recurrent herpetic disease. virus was isolated by cocultivation from active herpetic lesions (9 of 10) as well as from the footpads (17 of 38), sacral ganglia (7 of 21), and sciatic nerves (1 of 21) of asymptomatic animals. viral isolates grew in cells of human origin and were neutralized by hyperimmune anti hsv-2 sera. humoral imm ...19806254886
double infection of balb/c mice with rauscher murine leukemia and herpes simplex virus type 2.when balb/c mice were first infected with rauscher murine leukemia virus (mulv-r) and then superinfected with herpes simplex virus type 2 (hsv-2) the inhibition of the evolution of splenomegaly was observed. the effect did not depend on the route of hsv-2 administration. experiments in which potent anti-interferon serum was administered to double infected mice suggested that the antagonism between mulv-r and hsv-2 was not mediated by endogenous interferon, hsv-2 was found to replicate in the lps ...19806255890
interaction of herpesvirus with spleen cell subpopulations comparison of a neurotropic and a lymphotropic virus.we studied the interaction of a neurotropic herpesvirus, herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2), and a lymphotropic herpesvirus, guinea pig herpes-like virus (hlv), with guinea pig spleen cells. both hsv-1 and hsv-2 and hlv can attach to and penetrate into b- or t-enriched cells. less than 1.4% of the total b- or t-enriched cell populations were susceptible to infection by hlv and to some degree to hsv-1 or hsv-2 as determined by infectious center assays. after specific antiserum ...19806262239
oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition.5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ...19806248643
cervical carcinoma: detection of herpes simplex virus rna in cells undergoing neoplastic change.3h-hsv-2 dna has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. rna complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer.19806249760
quantification of plasminogen activator activity associated with herpesvirus-transformed cells.herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ...19806255081
immunoprecipitation of polypeptides from hamster embryo cells transformed by herpes simplex virus type 2. 19806249032
combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2.the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ...19806249191
influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine.the inhibition of herpes simplex virus type 1 (hsv-1) plaque formation by acycloguanosine (acg) was assayed in human fetal lung fibroblasts (hl), cell lines from human cervical carcinoma, rabbit cornea, and human rhabdomyosarcoma, and three green monkey kidney cell lines. the acg concentration giving 50% plaque reduction (pr50) of hsv-1 was lowest in hl. in two green monkey kidney cell lines, hsv-1 plaque formation was relatively insensitive to acg, with pr50 of 25 and 60 mum, respectively. in t ...19806265398
collagenase levels in a new model of experimental herpetic interstitial keratitis.a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ...19806246865
differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells.fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ...19806249749
comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2.we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ...19806255183
an evaluation of herpes simplex virus antigenic markers in the study of established and developing cervical neoplasia.previous reports from our laboratory have shown that antiserum to "pure" ag-e, a type-common hsv antigen, specifically stains atypical cervical cells in indirect immunofluorescence. these observations have been confirmed and extended. antisera were prepared against the two protein components of pure ag-e, designated icp 12 (m. w. = 140,000) and icp 14 (m. w. = 130,000), and were purified to radiochemical homogeneity by sds-acrylamide gel electrophoresis. the antisera reacted as well as antiserum ...19806255843
the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication.both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed.19806255077
effect of corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in balb/c mice.treatment of 4- to 6-week-old, 18- to 22-g male balb/c mice with 0.6 mg of corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (hsv-2). optimal protection occurred when c. granulosum was injected 1 week before hsv-2 infection. c. granulosum-induced resistance to hsv-2 lasted up to 4.5 weeks. studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated im ...19806262241
experimental studies on genital herpetic infection in mice.female icr mice were infected with hsv-1 and hsv-2 by inserting a cotton pellet soaked in viral solution (10(7-8) pfu/m1) into the vagina. the appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. these histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. results of fluorescent antibody studies on the appearance of vir ...19806266394
localization of the thymidine kinase gene of herpes simplex virus type 2 (333).the thymidine kinase (tk) gene of herpes simplex virus type 2 (hsv-2) has been identified on purified restriction endonuclease fragments of hsv-2 dna. these fragments were localized on the physical map of hsv-2 dna. a detailed map of the hsv-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). mouse tk- cells that were biochemically tr ...19806263824
expression of type-common envelope antigens by herpes simplex virus type 2-transformed hamster cells.at least ten polypeptides were detected on the surface of the herpes simplex virus type 1 (hsv-1) virion. two of these polypeptides, having molecular weights of 30,000 and 33,000, were identified in two herpes simplex virus type 2-transformed cell lines (333-8-9 and 333-2-29) by immunoprecipitation and sds-page. the same two virion polypeptides previously were shown to be present in an hsv-1-transformed cell line (14-012-8-1, t-10). these observations suggest that genetic information coding for ...19806259083
cloning of herpes simplex virus 2 dna fragments in a plasmid vector.dna isolated from virions of herpes simplex type 2 (hsv-2) strain 333 was digested with various restriction enzymes and joined to the ek2 plasmid vector pbr322. the viral dna sequences present in the hybrids were analyzed by restriction enzyme mapping and hybridization to fragments of hsv-2 dna. the collection of recombinant molecules represents approx. 75% of the hsv-2-genome. in most cases, the structure of the recombinants seemed identical to the organization of authentic fragments of hsv-2 d ...19806260572
depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced tumors.total hemolytic complement (ch50) levels were compared in sera from normal hamsters and hamsters bearing tumors derived from herpes simplex virus type 2-transformed cells ch50 in normal sera ranged from 160 to 212 while ch50 in tumor bearer sera ranged from 82 to 146. preincubation of tumor bearer sera with cell surface proteins (csp) from homologous herpes simplex virus type 2-derived tumor cells resulted in a 66% depletion of ch50 whereas preincubation with heterologous herpes simplex virus ty ...19806258785
new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea and tannic acid ointment]. part ii: female patients.clinical treatment of 147 female patients suffering with genital herpes simplex virus were conducted in two phases. the purpose of phase i was to note the efficacy of treatment with ultrasound and herpigon ointment (zinc, urea, and tannic acid) in relieving pain and shortening the length of time involved in healing. results indicated that the combined treatment significantly reduced pain and length of healing period. phase ii involved a 2-year follow-up of patients with primary and recurrent inf ...19806931874
topical therapeutic efficacy of 9-(2-hydroxyethoxymethyl)guanine and 5-iodo-5'-amino-2',5'-dideoxyuridine on oral infection with herpes simplex virus in mice.the therapeutic efficacy of two new antiviral agents, 5-iodo-5'-amino-2', 5'-dideoxyuridine (aidurd) and 9-(2-hydroxyethoxymethyl)guanine (acv), in the model of mouse lip inoculated with herpes simplex virus type 2 is reported. the effects on development of clinical lesions, viral replication in the inoculated lips, and establishment of latent viral infection in the trigeminal ganglia were observed. the earlier the treatment with aidurd and acv was initiated after inoculation, the better was the ...19806966305
analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus.peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. in contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. the effect was age dependent. macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for ...19807399667
relationship of antibody to outcome in neonatal herpes simplex virus infections.neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ...19807216423
estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method.a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ...19807219207
bilateral necrotizing retinitis complicating fatal encephalitis probably due to herpes simplex virus type 2.the clinical history, autopsy findings and ocular pathology of a case of localized neonatal herpes simplex virus (hsv) type 2 infection is reported in which the main brunt of the infection fell on the cerebral hemispheres and the peripheral retina. hsv type 2 was isolated from skin vesicles which developed on the trunk and arms and in the mouth 10 days after birth. there was no visceral involvement. although the infant was probably infected at some time during birth, hsv could not be isolated fr ...19807454228
properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype.we have isolated cells with a thymidine kinase-negative (tk(-)) phenotype from cells which carry the herpes simplex virus type 2 tk gene by selection in 5-bromodeoxyuridine or 9-(2-hydroxyethoxymethyl)guanine. both selection routines generated revertants with a frequency of 10(-3) to 10(-4), and resistance to either compound conferred simultaneous resistance to the other. tk(-) revertants fell into three classes: (i) cells that arose by deletion of all virus sequences, (ii) cells that had lost t ...198016789205
effect of herpes simplex virus type 2 and friend erythroleukemia virus infection of ige antibody responses to ascaris antigen in mice.the ige response to ascaris antigen, as measured by passive cutaneous anaphylactic reactions, was suppressed in mice infected with either herpes simplex virus type 2 (hsv-2) or friend leukemia virus (fv). fv was not suppressive unless infection preceded immunization whereas hsv-2 infection was immunosuppressive before or after immunization. however, the suppression by hsv-2 was relatively short-lived.19817228435
herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection.women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ...19816941038
[studies on the placental damage and iugr caused by intravaginally inoculated herpes simplex virus type-2 (hsv-2) during pregnancy (author's transl)].it has been recognized that intrauterine growth retardation (iugr) could be caused by viral infection during pregnancy. we have experimentally attempted the intravaginal inoculation of hsv-2 (uw-268) 1.2-2.0 x 10(6) plaque forming unit in the pregnant rat. hsv-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. the invading route of the virus was investigate ...19817234355
herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association.sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ...19817284955
antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin infection.win 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ...19817247370
viruses and gynecologic cancers: herpesvirus protein (icp 10/ag-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity.the studies associating infections by herpes simplex virus type 2 (hsv-2) with carcinoma of the human uterine cervix are reviewed within the context of three possible interpretations. extensive seroepidemiologic evidence indicates that the virus does not grow preferentially in neoplastic tissue, nor is the association of hsv-2 with cervical carcinoma a reflection of their independent relationship to promiscuity. while a number of infectious agents, including other viruses, are associated with ce ...19817023662
in-vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains.intra-peritoneal (i.p.) infection of mice with herpes simplex virus type 2 (hsv 2) attracted macrophages into the peritoneum. macrophages from moderately and highly hsv 2 resistant mouse strains expressed elevated phagocytosis activity 24 hours after injection. stimulation of phagocytosis in low resistant strains was generally less effective or absent. this was, in some experiments, due to the fact that macrophages were already highly activated before the experimental infection. i.p. infection a ...19817319542
expression of herpes simplex virus common surface antigens and malignancy by clonal cells of a herpes simplex virus type 2-transformed line.herpes simplex virus (hsv) type common surface antigens (csa) were examined by indirect immunofluorescence with rabbit antiserum to hsv type 1 in a clonal hamster cell line 155-4 transformed by hsv type 2. the tumor formation was examined in hamsters transplanted with various transformed and tumor cells. the examination of subclones derived from 1554-cell line gave the following results. (1) thirty subclones were isolated and classified into three phenotypes as to csa expression: (i) in csa-pos ...19816281115
herpes simplex virus type 2 and cervical cancer. 19816271502
latency in vitro using irradiated herpes simplex virus.human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ...19816267167
physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue.the physical mapping of six ts mutations of herpes simplex virus type 2 (hsv-2) is presented. the results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 hsv-1/hsv-2 intertypic recombinants. the order of these mutations on the physical map of hsv-2 is unambiguous and correlates almost exactly with the previously published genetic map of timbury & calder (1976). one of the mutants studied (hsv-2 ts12) has apparently two distinct conditionally lethal ts m ...19816267168
the detection of dna tumour virus-specific rna sequences in abnormal human cervical biopsies by in situ hybridization.we have used the technique of in situ nucleic acid hybridization and autoradiography of thin frozen sections of human tissue to search for virus rna sequences in human cervical tumours. of cervical biopsies with abnormal cytology, 67% bound herpes simplex virus type 2 (hsv2) 3h-labelled dna probes and 39% bound adenovirus type 2 (ad2) 3h-labelled dna probes, whereas control experiments with phage lambda 3h-labelled dna probes, under the same conditions, bound to only 7% of cases. in contrast, no ...19816271899
one functional copy of the long terminal repeat gene specifying the immediate-early polypeptide ie 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus.the hsv-1/hsv-2 intertypic recombinant bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (irl and trl) and expresses both the immediate-early polypeptide ie 110 of hsv-1 and its functionally equivalent polypeptide ie 118 of hsv-2. the genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. subclone 14 lacked most of the irl sequence, including the region from which part of the mrna ...19816271901
differential immunologic reactivity and processing of glycoproteins ga and gb of herpes simplex virus types 1 and 2 made in vero and hep-2 cells.herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) specify five major glycoproteins designated as ga, gb, gc, gd, and ge. previous studies have shown that ga and gb differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. moreover, ga and gb of hsv-1 crossreact in serologic tests with the corresponding glycoproteins of hsv-2. in this paper, we report on the reactivities of ga and gb of hsv-1 and hsv-2 with 24 independently de ...19816272294
consistent appearance of microtubules in cells productively infected with various strains of type 2 herpes simplex virus.electron microscopic examination showed microtubular structures in fl cells infected with all 18 strains of hsv-2 examined, but not in cells infected with 9 strains of hsv-1. these structures were also detected in other cultured cells (vero and earle's l cells) infected with hsv-2, and also in vivo in cells, such as neuronal cells of the spinal ganglia and liver cells, of one-day-old suckling mice (ddd strain) infected with this type of virus. thus the microtubules were consistently detected in ...19816272691
evaluation of the antiherpetic activity of 2'-fluoro-5-iodo-ara-c in rabbit eyes and cell cultures.2-fluoro-5-iodo-ara-c (fiac), a new and potent drug, was tested for antiviral activity against several strains of herpes simplex virus (hsv), types 1 and 2. effective dose-50% (ed-50) determinations for fiac ranged from 0.023 to 0.51 mum for hsv-2. fiac-treated cells did not exhibit any toxicity until the drug concentration was increased 2000-fold above the ed-50 level. ocular herpetic keratitis in new zealand white rabbits was treated with 1.0%, 0.1%, 0.01% fiac beginning 3 days after inoculati ...19816273355
antiherpes activity of [e]-5-(1-propenyl)-2'-deoxyuridine and 5-(1-propenyl)-1-beta-d-arabinofuranosyluracil.5-(1-propenyl)-1-beta-d-arabinofuranosyluracil has been synthesized, and this compound and [e]-5-(-propenyl)-2'-deoxyuridine have been tested for inhibition of herpes virus multiplication. only [e]-5-(1-propenyl)-2'-deoxyuridine was found to be an active inhibitor reducing by 50% the plaque formation of herpes simplex virus type 1 (hsv-1) at about 1 mum. a comparison to the bromovinyl derivatives showed the following order of descending activity; [e]-5-(2-bromovinyl)-2'-deoxyuridine greater than ...19816280606
[possible role of herpes simplex virus type 2 in the etiology of cervical cancer]. 19816274212
[experimental endophthalmitis induced by herpes simplex virus. 2. intracarotid inoculation (author's transl)]. 19816275689
the development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses.two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of mrc-5 and vero cells, and herpes simplex type 2 virus. cell growth was similar in both systems giving yields of 14 x 10(5)/cm2 in microcarrier and 18 x 10(5)/cm2 in glass sphere cultures with yields of vero cells being marginally better than mrc-5 cells. virus yields were only slightly lower in these cultures than in small scale stationary cultures ...19816281104
herpes simplex vaccine using cell membrane associated antigen in an animal model.vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of herpes simplex virus type 2 (hsv-2) infected vero cells with dithiothreitol and formaldehyde. these preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with hsv-2.19816281105
physical mapping of drug resistance mutations defines an active center of the herpes simplex virus dna polymerase enzyme.the genome structures of herpes simplex virus type 1 (hsv-1)/hsv-2 intertypic recombinants have been previously determined by restriction endonuclease analysis, and these recombinants and their parental strains have been employed to demonstrate that mutations within the hsv dna polymerase locus induce an altered hsv dna polymerase activity, exhibiting resistance to three inhibitors of dna polymerase. the viral dna polymerases induced by two recombinants and their parental strains were purified a ...19816270349
nonstructural proteins of herpes simplex virus. ii. major virus-specific dna-binding protein.the major herpes simplex virus type 2 dna-binding infected cell-specific polypeptides 11 and 12 have been purified to homogeneity from extracts of virus-infected cells. monospecific antiserum to the purified protein has been made and used to examine virus temperature-sensitive mutants for defects in the synthesis of the protein and to probe virus dna synthesis in isolated chromatin. the purified protein acted directly on a polydeoxyadenylic acid-polydeoxythymidylic acid helix, reducing its melti ...19816270358
identification of a virus-specific polypeptide associated with a transforming fragment (bglii-n) of herpes simplex virus type 2 dna.the bglii n fragment of herpes simplex virus type 2 (hsv-2) dna (approximately 0.58 to 0.63 map unit) was examined for encoded products. using plasmid pgz59, which consists of bglii-n cloned in pat153, in conjunction with hybrid arrested translation, mrna selection, and in vitro protein synthesis, we found that the major translated product of this region has an approximate molecular weight of 37,800. by further mapping, coding sequences for this polypeptide were located within the region of bgli ...19816270369
pathogenesis of herpes simplex virus infections in guinea pigs.the pathogenesis of herpes simplex virus types 1 and 2 has been studied in guinea pigs after inoculation by various routes (subcutaneous and intradermal infection in footpads and vaginal infection). clinical observations as well as virus isolation studies are reported. herpes simplex virus type 2 infection by all three routes of inoculation led to acute primary and recurrent lesions. virus persisted in the nervous system, particularly in sensory ganglia, and locally at the site of inoculation. h ...19816277787
herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication.in addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ...19816270377
cell-mediated immunity to herpes simplex virus: recognition of type-specific and type-common surface antigens by cytotoxic t cell populations.in this communication, we examine the specificity of anti-herpes simplex virus (hsv) cytotoxic t lymphocytes (ctl). serological studies of the two related hsv serotypes (hsv-1 and hsv-2) have revealed both type-specific and cross-reactive antigenic determinants in the viral envelope and on the surface of infected cells. by analysis of cytotoxicity of ctl, generated in vitro by restimulation of splenocytes from mice primed with one or the other hsv serotype, the recognition of both type-specific ...19816277790
specific lymphocyte blastogenic responses in children with cytomegalovirus and herpes simplex virus infections acquired early in infancy.cell-mediated immune responses in 27 infants and children with cytomegalovirus (cmv) infection acquired between birth and 1 year of age were compared with responses in 13 children who had neonatal herpes simplex virus (hsv) infection. infection was asymptomatic in 25 of 27 cmv-infected children; the 13 patients with hsv infection were all ill as newborns. the median age when studied was 46 months for children infected with cmv and 24 months for those infected with hsv. we measured lymphocyte tra ...19816271679
use of monoclonal antibody directed against herpes simplex virus glycoproteins to protect mice against acute virus-induced neurological disease.monoclonal antibodies hcl and hd1, directed against herpes simplex virus type 1 (hsv-1) glycoproteins gc and gd, respectively, were evaluated for their ability to passively immunize mice against acute virus-induced neurological disease after footpad inoculation with hsv-1 or herpes simplex virus type 2 (hsv-2). control virus-infected mice receiving a single intraperitoneal injection of normal serum died within 7 to 10 days after the spread of virus from footpad to spinal cord and brain. however, ...19816271681
inability to rescue viral genes from human cells biochemically transformed by herpes simplex virus type 2 dna. 19816274496
molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection.in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ...19816275122
[use of a contraceptive suppository as chemoprophylaxis against sexually-transmitted diseases].the incidence of sexually transmitted diseases (std), especially gonorrhea, has risen dramatically in the past 2 decades. the population at greatest risk is also at high risk for unwanted pregnancies. since currently employed treatment methods are failing to curb the problem, a search was instituted for an available vaginal contraceptive that would have a prophylactic effect against these diseases. in vitro studies have indicated that a vaginal contraceptive made of phenylmercuric acetate is ...19816277015
antibody responses in humans to individual proteins of herpes simplex viruses.sera from 231 women were used to examine their frequency of precipitation of various herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) proteins and to determine if there was a rank order of immune responsiveness of humans to these hsv antigens. radiolabeled viral proteins were reacted with serum and immune complexes isolated with staphylococcal protein a. individual antigens were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. as a group, these sera precipitated ...19816277791
mode of action of phosphonoformate as an anti-herpes simplex virus agent.phosphonoformate inhibited the replication of herpes simplex virus (hsv) type 1 and type 2 in culture. the concentration required to inhibit the replication of both types of virus by 2 logs at 28 h post-infection was approximately 150 microm. it was more potent than phosphonoacetate against the growth of both virus types. a virus mutant which is resistant to phosphonoacetate was cross-resistant to phosphonoformate. arsonoacetate, at 300 microm, had no antivirus activity. phosphonoformate also in ...19816260189
immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper.in this paper we report that viral polypeptides from herpes simplex virus 1 (hsv-1) and 2 (hsv-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. the polyvalent hyperimmune sera against hsv-1 reacted with 17 hsv-1 polypeptide bands and 8 hsv-2 polypeptide bands. concordantly, polyvalent sera against hsv-2 ...19816260673
induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2.a series of studies were performed to evaluate the oncogenic potential of inactivated herpes simplex viruses types 1 (hsv-1) and 2 (hsv-2) in the mouse cervix. hsv-1 or hsv-2 prepared in hep-2 cell cultures and inactivated by exposure to formalin or ultraviolet light was applied to the mouse cervix for periods ranging from 20 to 90 weeks. control mice were exposed for the same period to control fluids. vaginal cytologic preparations from all animals were examined weekly to detect epithelial abno ...19816269724
molecular genetics of herpes simplex virus. vii. characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in dna synthesis and accumulation of gamma polypeptides.we report on the properties of a temperature-sensitive mutant produced by transfection of cells with intact dna and a specific dna fragment mutagenized with low levels of hydroxylamine. the plating efficiency of the mutant at 39 degrees c relative to that at 33.5 degrees c was 5 x 10(-6). the pattern of polypeptides produced at the nonpermissive temperature was similar to that seen with wild-type virus in infected cells treated with inhibitory concentrations of phosphonoacetic acid in that alpha ...19816260973
recovery of mice from herpes simplex virus type 2 hepatitis: adoptive transfer of recovery with immune spleen cells.young balb/c mice inoculated intraperitoneally with herpes simplex virus type 2 develop focal necrotizing hepatitis. after infection, the livers of these mice show increasing virus titers, which reach a maximum on day 3 after infection; this is followed by a dramatic decrease in the amount of virus recovered on days 4 and 5. this decrease in virus content is accompanied by a progressive infiltration of the lesions with mononuclear leukocytes and an apparent resolution of the lesions. adoptive tr ...19816269998
[new antigens induced by herpes simplex virus in human tumors].tissues of malignant tumors of the genitalia (cervix, uterus body, ovary) contain a specific antigen associated with herpes simplex virus type 2 (hsv-2) identical with the antigen of the infected cells. the virus-induced antigen was detected in tissues of cervical carcinoma in 35% of cases, in 15% of tumors of the corpus uteri and in 13% of ovary tumors. hsv-2 was isolated from pathologically altered cervical carcinoma cells in 2 out of 56 cases examined. these facts indicate the presence of hsv ...19816267821
"the herpesvirus hypothesis"--are koch's postulates satisfied? 19816273266
[studies on the nature of tubular structures as a marker of herpes simplex virus type 2 (author's transl)]. 19816273274
kaposi's sarcoma. iv. detection of cmv dna, cmv rna and cmna in tumor biopsies.in order to determine whether human cytomegalovirus- (cmv) dna homologous sequences as well as cmv-specific rna(s) and antigen(s) exist in tumor biopsies of kaposi's sarcoma (ks) dna-dna reassociation, rna-dna in situ cytohybridization and anticomplement immunofluorescence test (acif) tests were applied. three of 10 dnas extracted from kaposi sarcoma biopsies contained dna sequences homologous to radioactively labelled human cmv dna probe. the amount of cmv dna in these sarcoma tissues was calcu ...19816273333
assay of type-specific and type-common antibodies to herpes simplex virus types 1 and 2 in human sera.a reliable and reproducible method for determining specific reactivity to herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) in human sera has been developed. human sera were used to immunoprecipitate hsv-specific glycoprotein antigens from both hsv-1- and hsv-2-infected cell extracts. the viral glycoproteins precipitated from these extracts were then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to detect specific reactivity of the sera with distinct type-specific anti ...19816262247
differences in neurovirulence among isolates of herpes simplex virus types 1 and 2 in mice using four routes of infection.differences in neurovirulence between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using recent clinical isolates and laboratory-passaged strains in intravaginal, intranasal, intraperitoneal, and intracerebral infections of mice. the hsv-2 isolates caused higher death rates in all four infections. no differences in death rate were observed between recent and passaged isolates of either hsv-1 or hsv-2. after intravaginal inoculation, hsv-1 isolates replicated to higher ...19816273475
repression and activation of the genome of herpes simplex viruses in human cells.we have described previously a cell culture system in which the herpes simplex virus (hsv) type 2 (hsv-2) genome is maintained in a repressed form after treatment of infected cells with 1-beta-d-arabinofuranosylcytosine and increase of incubation temperature from 37 degrees c to 39.5 degrees c. infectious hsv-2 production was activated by altering incubation temperature or by superinfecting with human cytomegalovirus. we now report the establishment of an analogous system utilizing hsv type 1 (h ...19816273875
conversion of herpetic lesions to malignancy by ultraviolet exposure and promoter application.many lines of evidence exist associating herpes simplex virus (hsv) with the development of carcinoma, but much of this evidence is anecdotal or associative in nature and does not prove a cause and effect. the purpose of this research was to investigate the oncogenic potential of hsv type 2 (hsv-2) in vivo. a mouse model for lip carcinogenesis was designed to combine hsv-2 infection, u.v. exposure and tetradecanoyl-phorbol-acetate (tpa) application. hsv-2 inoculation on to abraded mouse lips was ...19816270265
protectivity of herpes simplex virus antigens: studies in mice on the adjuvant effect of piclc and on the dependence of protection on t cell competence.the efficacy of a herpes simplex virus type 1 (hsv-1) envelope antigen (eag) preparation against hsv infection was studied in t cell competent and t cell deficient mice. immuno-competent mice were successfully protected against herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a hsv-1 envelope antigen (hsv-1 eag) preparation. since hsv-1 eag was considerably less effective than the who ...19816268955
characterization of rat embryo cells transformed by ts mutants and sheared dna of herpes simplex virus types 1 and 2 and a derived tumor cell line.four independent isolated lines transformed by ts mutant or sheared dna of herpes simplex virus types 1 and 2 and one tumor cell line were characterized according to their behavior in assays of aggregation, growth in low serum, doubling times, saturation density, agglutination by lectins, uptake of metabolites, and production of plasminogen activator. the criteria used to select the transformed cells used in this study were the formation of altered foci of cells followed by the formation of a co ...19816263467
induction of natural killer cells by herpes-simplex virus type 2 in resistant and sensitive inbred mouse strains.infection of mice with herpes simplex virus type 2 (hsv 2) stimulated natural killer (nk) cells in a variety of inbred mouse strains including athymic nude mice. essentially all mouse strains tested exhibited high nk activity on day four after virus inoculation. assayed 24 hours after infection, swr/j, akr/j, sjl/j and c57b1/10j mice were low or negative for these non-virus-specific cytotoxic responses. whereas the first two mouse strains were most sensitive to the lethal effects of hsv 2, the l ...19816263793
Displaying items 401 - 500 of 7420