Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| tubular structures in the cervix of mice experimentally infected with herpes simplex virus type 2. | experimental infection of the c3h/n mouse genital tract was demonstrated after intravaginal inoculation with herpes simplex virus type 2 (hsv-2). about 75% of the infected animals died by day 7, and 75% of the surviving animals had severe vaginitis or neurological signs on day 7. titers of the virus recovered from vaginal secretions of infected animals reached a maximum on day 2 and gradually decreased until day 7. on the other hand, under the electron microscope, virus particles and tubular str ... | 1980 | 6246195 |
| tumorigenic transformation induced by a specific fragment of dna from herpes simplex virus type 2. | transfection of syrian hamster embryo cells with limit digests of bgl ii-, hpa i-, or bgl ii/hpa i-cleaved dna from herpes simplex virus type 2 (strain s-1) but not with salmon sperm dna resulted in the appearance of refractile, morphologically altered cells at a frequency of 10(-5)/0.005 microgram of viral dna within two to four passages. transformed lines manifested reduced serum requirement and anchorage-independent growth and were tumorigenic in newborn hamsters. they expressed icp10, a vira ... | 1980 | 6246534 |
| macrophage extrinsic antiviral activity during herpes simplex virus infection. | peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ... | 1980 | 6247426 |
| comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice. | the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ... | 1980 | 6247441 |
| differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. | fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ... | 1980 | 6249749 |
| immunity to herpes simplex virus type 2 (hsv-2). i. development of virus-specific lymphoproliferative and leukocyte migration inhibition factor responses in hsv-2-infected guinea pigs. | 1980 | 6258811 | |
| oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | 5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ... | 1980 | 6248643 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2. | the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ... | 1980 | 6249191 |
| [effect of the physiological status of culture on herpes simplex virus type 2 infection of synchronized cells bnk-21]. | infecting of synchronized cells-bnk-21 by herpes simplex virus type 2 in the s period resulted in a secondary rise of the labeled cells index and shortened time of the cell cycle as a whole. while infecting of the cells during the s period some morphological transformation was observed. the infecting of the cells in the periods m, g1, g2 revealed no essential changes in cell dna synthesis and failed to result in any cell transformation. | 1980 | 6250284 |
| temporal regulation of herpes simplex virus type 2 transcription and characterization of virus immediate early mrna's. | nuclear and cytoplasmic virus rnas, synthesized in cells infected with herpes simplex virus type 2 at early and late times post-infection, and in the continuous presence of the protein synthesis inhibitor cycloheximide (immediate early), have been analyzed by blot hybridization to virus dna fragments generated by bam hi and eco ri restriction endonucleases. polyadenylated immediate early mrnas were separated on denaturing gels containing ch3hgoh giving three virus-specific mrna bands of estimate ... | 1980 | 6253886 |
| responses of mouse spleen morphology to the growth of subcutaneously injected virally transformed cells. | the changes in the fractional volume of six structural components in the spleens of balb/c mice injected with herpes simplex virus type 2-transformed cells (h238 tumor cells) were quantitated during progressive tumor growth. spleen stereology was performed at three time intervals during the early stages of tumor development. the results revealed that the volume of the compact myeloid tissue and reaction center of lymphoid nodules increased about four- to five-fold from 10 to 33 days after h238 t ... | 1980 | 6254396 |
| structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. | capsids of herpes simplex virus (hsv) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed nc-1 through nc-7 in order of descending molecular weight). antibodies prepared to hsv-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against hsv-1-infected kb cells. three of the antibodies (anti-nc-1, anti-nc-2, and anti-nc-3,4) also reacted with hsv-2-infected cells. tryptic peptide anal ... | 1980 | 6154808 |
| glycoproteins with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus types 1 and 2. | agar immunodiffusion tests demonstrated that bhk 21 cells infected with either hsv i or hsv 2 release only a few hsv-specified antigens into the extracellular fluid (infected cell released polypeptides-icrp). neutralization blocking experiments showed that the majority of antigens/(including the band ii common antigen) involved as target sites in antibody-mediated virus neutralization are present in the icrp of both hsv i and hsv 2.sds-page identified six regions of virus-specified proteins in t ... | 1980 | 6156995 |
| association of herpes simplex thymidine kinase gene with chromosome no. 18 in transformed human cells. | the herpes simplex virus type-2 (hsv-2)-transformed human cell line hb-2-3 was fused with thymidine kinase (tk)-deficient mouse cells [lm(tk-)], and 12 independent hybrids were isolated with the use of the hat (hypoxanthine, amethopterin, and thymidine)-ouabain selection system. discontinuous polyacrylamide gel electrophoresis studies demonstrated that the hsv-2-specific tk was the selected enzyme in the hybrids. isoenzyme analysis and karyotyping were used in the analysis of the hybrids for the ... | 1980 | 6243715 |
| characterization of herpes simplex virus strains isolated from patients with various diseases. | herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ... | 1980 | 6243918 |
| different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits. | skin infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ... | 1980 | 6244227 |
| ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2. | the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ... | 1980 | 6249764 |
| a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein. | a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ... | 1980 | 6244352 |
| analysis of viral dna sequences in hamster cells transformed by herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) dna was treated with four restriction endonucleases (ecori, hinciii, bgl ii, and xba i) and eight fragments were purified and labeled with 32p in vitro. the kinetics of renaturation of each of the fragments was measured in the presence of dna extracted from 333-8-9, a hamster cell line transformed by uv light-inactivated hsv-2 strain 333, and from a series of cloned derivatives and their tumor lines. all of the lines examined contained a partial set of viral s ... | 1980 | 6244590 |
| interaction of herpesvirus with spleen cell subpopulations comparison of a neurotropic and a lymphotropic virus. | we studied the interaction of a neurotropic herpesvirus, herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2), and a lymphotropic herpesvirus, guinea pig herpes-like virus (hlv), with guinea pig spleen cells. both hsv-1 and hsv-2 and hlv can attach to and penetrate into b- or t-enriched cells. less than 1.4% of the total b- or t-enriched cell populations were susceptible to infection by hlv and to some degree to hsv-1 or hsv-2 as determined by infectious center assays. after specific antiserum ... | 1980 | 6262239 |
| pathogenicity of wild-type and temperature-sensitive mutants of herpes simplex virus type 2 in guinea pigs. | the pathogenicity of herpes simplex virus type 2 strain 186, the wild-type (wt) strain, and four temperature-sensitive (ts) mutants was studied after genital inoculation of female guinea pigs. infection with the wt virus was generally severe, with extensive skin lesions in 89% and mortality in 37% of inoculated animals. guinea pigs inoculated with ts mutants manifest remarkably mild disease, with lesions occurring in only 16% of the guinea pits and a mortality rate of 7%. wt virus was recovered ... | 1980 | 6254877 |
| presence of herpes simplex virus type 2 (hsv-2) antibodies and antigens in patients with cervical carcinoma in thailand. | 1980 | 6255050 | |
| herpes simplex virus infection: problems and prospects as perceived by a peripatetic pediatrician. | the multivaried aspects of the herpes simplex viruses (hsv) types 1 and 2 and the infections they produce are discussed. points emphasized are: (1) the need for considering these (and other viruses) from an evolutionary perspective; (2) the necessity of disseminating current methods for virus identification; (3) the great progress in molecular-virological aspects and in the genetics of the virus which provide new tools for epidemiological and immunological studies and define more convincingly th ... | 1980 | 6246686 |
| collagenase levels in a new model of experimental herpetic interstitial keratitis. | a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ... | 1980 | 6246865 |
| comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections. | indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ... | 1980 | 6254476 |
| elution of herpes simplex virus-specific cytotoxic antibodies from squamous cell carcinoma of uterine cervix. | there is evidence that carcinoma of the cervix uteri is associated with infection with herpes simplex virus type 2 (hsv-2), and although indirect evidence suggests an aetiological relationship, this remains controversial. one facet of the problem is that complement-dependent cytotoxic antibodies is hsv-2-infected cells have been undetectable or found only in low titre in sera from patients with progressive cervical cancer whereas higher titres have been found where cervical lesions are less adva ... | 1980 | 6250079 |
| reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls. | serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ... | 1980 | 6254649 |
| the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication. | both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed. | 1980 | 6255077 |
| properties of herpes simplex virus type 1 and type 2 dna polymerase. | herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ... | 1980 | 6250618 |
| quantification of plasminogen activator activity associated with herpesvirus-transformed cells. | herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ... | 1980 | 6255081 |
| cervical carcinoma: detection of herpes simplex virus rna in cells undergoing neoplastic change. | 3h-hsv-2 dna has been hybridized in situ to frozen sectioned tissue from human cervical biopsies. rna complementary to the virus-specific probe was detected in cells undergoing pre-malignant changes, but not in the cells of the fully developed squamous-cell cancer. | 1980 | 6249760 |
| comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2. | we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ... | 1980 | 6255183 |
| double infection of balb/c mice with rauscher murine leukemia and herpes simplex virus type 2. | when balb/c mice were first infected with rauscher murine leukemia virus (mulv-r) and then superinfected with herpes simplex virus type 2 (hsv-2) the inhibition of the evolution of splenomegaly was observed. the effect did not depend on the route of hsv-2 administration. experiments in which potent anti-interferon serum was administered to double infected mice suggested that the antagonism between mulv-r and hsv-2 was not mediated by endogenous interferon, hsv-2 was found to replicate in the lps ... | 1980 | 6255890 |
| comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains. | this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ... | 1980 | 6246854 |
| influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine. | the inhibition of herpes simplex virus type 1 (hsv-1) plaque formation by acycloguanosine (acg) was assayed in human fetal lung fibroblasts (hl), cell lines from human cervical carcinoma, rabbit cornea, and human rhabdomyosarcoma, and three green monkey kidney cell lines. the acg concentration giving 50% plaque reduction (pr50) of hsv-1 was lowest in hl. in two green monkey kidney cell lines, hsv-1 plaque formation was relatively insensitive to acg, with pr50 of 25 and 60 mum, respectively. in t ... | 1980 | 6265398 |
| "in vivo" and "in vitro" effects of a bacterial extract on herpes simplex virus. | the present studies were designed to evaluate the effects of substance or substances extracted from escherichia coli on herpes simplex virus. the "in vivo" assays show that bacterial extract introduced i.p. in mice simultaneously with hsv2 brought about 100% of survival, but the inoculation of crude extract after virus challenge brought about complete mortality of mice. "in vitro" assays show that the crude extract reduced significantly the numbers of pfu; better results were obtained when the c ... | 1980 | 6255968 |
| immunity to herpes simplex virus type 2: cell-mediated immunity in latently infected guinea pigs. | cell-mediated (cmi) and humoral immunity to herpes simplex virus type 2 (hsv-2) were evaluated in infected strain 13/n guinea pigs with (45%) and without a history of recurrent herpetic disease. virus was isolated by cocultivation from active herpetic lesions (9 of 10) as well as from the footpads (17 of 38), sacral ganglia (7 of 21), and sciatic nerves (1 of 21) of asymptomatic animals. viral isolates grew in cells of human origin and were neutralized by hyperimmune anti hsv-2 sera. humoral imm ... | 1980 | 6254886 |
| anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy. | e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ... | 1980 | 6263181 |
| expression of type-common envelope antigens by herpes simplex virus type 2-transformed hamster cells. | at least ten polypeptides were detected on the surface of the herpes simplex virus type 1 (hsv-1) virion. two of these polypeptides, having molecular weights of 30,000 and 33,000, were identified in two herpes simplex virus type 2-transformed cell lines (333-8-9 and 333-2-29) by immunoprecipitation and sds-page. the same two virion polypeptides previously were shown to be present in an hsv-1-transformed cell line (14-012-8-1, t-10). these observations suggest that genetic information coding for ... | 1980 | 6259083 |
| localization of the thymidine kinase gene of herpes simplex virus type 2 (333). | the thymidine kinase (tk) gene of herpes simplex virus type 2 (hsv-2) has been identified on purified restriction endonuclease fragments of hsv-2 dna. these fragments were localized on the physical map of hsv-2 dna. a detailed map of the hsv-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). mouse tk- cells that were biochemically tr ... | 1980 | 6263824 |
| effect of corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in balb/c mice. | treatment of 4- to 6-week-old, 18- to 22-g male balb/c mice with 0.6 mg of corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (hsv-2). optimal protection occurred when c. granulosum was injected 1 week before hsv-2 infection. c. granulosum-induced resistance to hsv-2 lasted up to 4.5 weeks. studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated im ... | 1980 | 6262241 |
| depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced tumors. | total hemolytic complement (ch50) levels were compared in sera from normal hamsters and hamsters bearing tumors derived from herpes simplex virus type 2-transformed cells ch50 in normal sera ranged from 160 to 212 while ch50 in tumor bearer sera ranged from 82 to 146. preincubation of tumor bearer sera with cell surface proteins (csp) from homologous herpes simplex virus type 2-derived tumor cells resulted in a 66% depletion of ch50 whereas preincubation with heterologous herpes simplex virus ty ... | 1980 | 6258785 |
| cloning of herpes simplex virus 2 dna fragments in a plasmid vector. | dna isolated from virions of herpes simplex type 2 (hsv-2) strain 333 was digested with various restriction enzymes and joined to the ek2 plasmid vector pbr322. the viral dna sequences present in the hybrids were analyzed by restriction enzyme mapping and hybridization to fragments of hsv-2 dna. the collection of recombinant molecules represents approx. 75% of the hsv-2-genome. in most cases, the structure of the recombinants seemed identical to the organization of authentic fragments of hsv-2 d ... | 1980 | 6260572 |
| experimental studies on genital herpetic infection in mice. | female icr mice were infected with hsv-1 and hsv-2 by inserting a cotton pellet soaked in viral solution (10(7-8) pfu/m1) into the vagina. the appearance of giant cells and formation of intranuclear inclusions were detected in the epithelial layer of the uterus 24 h after intravaginal inoculation. these histopathological changes were pronounced 3 to 4 days after virus inoculation and then gradually disappeared in the next few days. results of fluorescent antibody studies on the appearance of vir ... | 1980 | 6266394 |
| new treatment for herpes simplex virus type 2 [ultrasound and zinc, urea and tannic acid ointment]. part ii: female patients. | clinical treatment of 147 female patients suffering with genital herpes simplex virus were conducted in two phases. the purpose of phase i was to note the efficacy of treatment with ultrasound and herpigon ointment (zinc, urea, and tannic acid) in relieving pain and shortening the length of time involved in healing. results indicated that the combined treatment significantly reduced pain and length of healing period. phase ii involved a 2-year follow-up of patients with primary and recurrent inf ... | 1980 | 6931874 |
| topical therapeutic efficacy of 9-(2-hydroxyethoxymethyl)guanine and 5-iodo-5'-amino-2',5'-dideoxyuridine on oral infection with herpes simplex virus in mice. | the therapeutic efficacy of two new antiviral agents, 5-iodo-5'-amino-2', 5'-dideoxyuridine (aidurd) and 9-(2-hydroxyethoxymethyl)guanine (acv), in the model of mouse lip inoculated with herpes simplex virus type 2 is reported. the effects on development of clinical lesions, viral replication in the inoculated lips, and establishment of latent viral infection in the trigeminal ganglia were observed. the earlier the treatment with aidurd and acv was initiated after inoculation, the better was the ... | 1980 | 6966305 |
| estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method. | a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ... | 1980 | 7219207 |
| analysis of the inhibitory effect of peritoneal macrophages on the spread of herpes simplex virus. | peritoneal macrophages obtained from mice after an intraperitoneal injection of tryptose peptone inhibited the development of herpes simplex virus type 2 plaques in syngeneic mouse embryonic fibroblasts. in contrast, peritoneal macrophages, spleen cells, and thymocytes from untreated mice showed only a minimal inhibitory effect on the development of viral plaques. the effect was age dependent. macrophages from 2 and 3-week-old mice showed weaker functions, requiring a larger number of cells for ... | 1980 | 7399667 |
| bilateral necrotizing retinitis complicating fatal encephalitis probably due to herpes simplex virus type 2. | the clinical history, autopsy findings and ocular pathology of a case of localized neonatal herpes simplex virus (hsv) type 2 infection is reported in which the main brunt of the infection fell on the cerebral hemispheres and the peripheral retina. hsv type 2 was isolated from skin vesicles which developed on the trunk and arms and in the mouth 10 days after birth. there was no visceral involvement. although the infant was probably infected at some time during birth, hsv could not be isolated fr ... | 1980 | 7454228 |
| relationship of antibody to outcome in neonatal herpes simplex virus infections. | neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ... | 1980 | 7216423 |
| properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene: mechanisms of reversion to a thymidine kinase-negative phenotype. | we have isolated cells with a thymidine kinase-negative (tk(-)) phenotype from cells which carry the herpes simplex virus type 2 tk gene by selection in 5-bromodeoxyuridine or 9-(2-hydroxyethoxymethyl)guanine. both selection routines generated revertants with a frequency of 10(-3) to 10(-4), and resistance to either compound conferred simultaneous resistance to the other. tk(-) revertants fell into three classes: (i) cells that arose by deletion of all virus sequences, (ii) cells that had lost t ... | 1980 | 16789205 |
| [studies on the placental damage and iugr caused by intravaginally inoculated herpes simplex virus type-2 (hsv-2) during pregnancy (author's transl)]. | it has been recognized that intrauterine growth retardation (iugr) could be caused by viral infection during pregnancy. we have experimentally attempted the intravaginal inoculation of hsv-2 (uw-268) 1.2-2.0 x 10(6) plaque forming unit in the pregnant rat. hsv-2 inoculated at 8th and 11th day of gestation, the virus invaded in the basal layer of placenta after 15th day of gestation, but no evidence of intrauterine infection on fetus was identified. the invading route of the virus was investigate ... | 1981 | 7234355 |
| in-vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains. | intra-peritoneal (i.p.) infection of mice with herpes simplex virus type 2 (hsv 2) attracted macrophages into the peritoneum. macrophages from moderately and highly hsv 2 resistant mouse strains expressed elevated phagocytosis activity 24 hours after injection. stimulation of phagocytosis in low resistant strains was generally less effective or absent. this was, in some experiments, due to the fact that macrophages were already highly activated before the experimental infection. i.p. infection a ... | 1981 | 7319542 |
| antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin infection. | win 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ... | 1981 | 7247370 |
| viruses and gynecologic cancers: herpesvirus protein (icp 10/ag-4), a cervical tumor antigen that fulfills the criteria for a marker of carcinogenicity. | the studies associating infections by herpes simplex virus type 2 (hsv-2) with carcinoma of the human uterine cervix are reviewed within the context of three possible interpretations. extensive seroepidemiologic evidence indicates that the virus does not grow preferentially in neoplastic tissue, nor is the association of hsv-2 with cervical carcinoma a reflection of their independent relationship to promiscuity. while a number of infectious agents, including other viruses, are associated with ce ... | 1981 | 7023662 |
| effect of herpes simplex virus type 2 and friend erythroleukemia virus infection of ige antibody responses to ascaris antigen in mice. | the ige response to ascaris antigen, as measured by passive cutaneous anaphylactic reactions, was suppressed in mice infected with either herpes simplex virus type 2 (hsv-2) or friend leukemia virus (fv). fv was not suppressive unless infection preceded immunization whereas hsv-2 infection was immunosuppressive before or after immunization. however, the suppression by hsv-2 was relatively short-lived. | 1981 | 7228435 |
| herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association. | sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ... | 1981 | 7284955 |
| herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection. | women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ... | 1981 | 6941038 |
| expression of herpes simplex virus common surface antigens and malignancy by clonal cells of a herpes simplex virus type 2-transformed line. | herpes simplex virus (hsv) type common surface antigens (csa) were examined by indirect immunofluorescence with rabbit antiserum to hsv type 1 in a clonal hamster cell line 155-4 transformed by hsv type 2. the tumor formation was examined in hamsters transplanted with various transformed and tumor cells. the examination of subclones derived from 1554-cell line gave the following results. (1) thirty subclones were isolated and classified into three phenotypes as to csa expression: (i) in csa-pos ... | 1981 | 6281115 |
| the development and use of microcarrier and glass sphere culture techniques for the production of herpes simplex viruses. | two high productivity monolayer culture methods, the microcarrier and glass sphere culture, were compared for their capacity to support the growth of mrc-5 and vero cells, and herpes simplex type 2 virus. cell growth was similar in both systems giving yields of 14 x 10(5)/cm2 in microcarrier and 18 x 10(5)/cm2 in glass sphere cultures with yields of vero cells being marginally better than mrc-5 cells. virus yields were only slightly lower in these cultures than in small scale stationary cultures ... | 1981 | 6281104 |
| herpes simplex vaccine using cell membrane associated antigen in an animal model. | vesicles of plasma membrane, containing no detectable live virus, were prepared by treatment of herpes simplex virus type 2 (hsv-2) infected vero cells with dithiothreitol and formaldehyde. these preparations proved to be antigenic on injection into guinea-pigs and also protected these animals against intravaginal challenge with hsv-2. | 1981 | 6281105 |
| transformation of rodent cells by a cloned dna fragment of herpes simplex virus type 2. | transformation of rodent cells with isolated restriction endonuclease fragments of herpes simplex virus type 2 dna identified a region of the genome located between map positions 0.58 and 0.62. these sequences were cloned into pbr322, and the recombinant plasmid was used to transform primary rat embryo cells and nih 3t3 cells. the transformants were selected for their ability to form dense foci on a monolayer or to form colonies in semisolid medium. in contrast to the parental rat or mouse cells ... | 1981 | 6264141 |
| differentiation of members of the human herpesviridae family by radioimmunoassay. | many individuals who are seronegative for one member of the human herpesviridae family are strongly seropositive for other members. using sera from such individuals, the radioimmunoassay technique demonstrated absence of antigen-antibody cross-reactions between varicella-zoster virus (vzv) and herpes simplex virus (hsv) at levels of less than one part in 1,000. sera containing antibody to both hsv and vzv were absorbed with antigens of one agent without significantly altering the amount of remai ... | 1981 | 6268545 |
| immunological reactivity of herpes simplex virus 1 and 2 polypeptides electrophoretically separated and transferred to diazobenzyloxymethyl paper. | in this paper we report that viral polypeptides from herpes simplex virus 1 (hsv-1) and 2 (hsv-2)-infected cells electrophoretically separated in sodium dodecyl sulfate-polyacrylamide-agarose gels and transferred to diazobenzyloxymethyl paper can react with rabbit hyperimmune sera, both polyvalent and prepared against specific antigens. the polyvalent hyperimmune sera against hsv-1 reacted with 17 hsv-1 polypeptide bands and 8 hsv-2 polypeptide bands. concordantly, polyvalent sera against hsv-2 ... | 1981 | 6260673 |
| mode of action of phosphonoformate as an anti-herpes simplex virus agent. | phosphonoformate inhibited the replication of herpes simplex virus (hsv) type 1 and type 2 in culture. the concentration required to inhibit the replication of both types of virus by 2 logs at 28 h post-infection was approximately 150 microm. it was more potent than phosphonoacetate against the growth of both virus types. a virus mutant which is resistant to phosphonoacetate was cross-resistant to phosphonoformate. arsonoacetate, at 300 microm, had no antivirus activity. phosphonoformate also in ... | 1981 | 6260189 |
| production of hybrid cell lines secreting antibodies to herpes simplex virus type 2. | following different immunization schedules we have produced 102 myeloma--spleen cell hybrid lines (30.8% of the total) secreting antibodies to hsv-2 infected cells. the highest yield of hybrids was derived from fusion of myeloma cells with spleen cells from mice sensitized by footpad injection of formalin-inactivated virus and boosted intravenously with the same antigen. to date, twelve of the antibody-producing hybrids have been cloned by limiting dilutions to generate over 100 monoclonal lines ... | 1981 | 6262346 |
| herpesviruses and parkinsonism. herpes simplex virus types 1 and 2, and cytomegalovirus antibodies in serum and csf. | antibodies against herpes simplex virus (hsv) types 1 and 2 and cytomegalovirus (cmv) were assayed with a microindirect hemagglutination (iha) test in the serum of 67 pairs of patients with parkinson's disease and controls. cerebrospinal fluid from 30 pairs was assayed. all patient and control serum was tested with a radioimmunoassay (ria) for antibodies against hsv type 1 subunit antigens. serum iha antibody level against hsv type 1 was increased in patients with parkinson's disease and ria ant ... | 1981 | 6257211 |
| linkage map of cotransfected viral dna sequences in a mouse 3t3 cell line. | a physical map was constructed of a cotransformed 3t3 thymidine kinase (tk) negative cell line revealing a close linkage of the selectable herpes simplex virus type 2 tk gene and cotransfected simian virus 40 dna sequences in high-molecular-weight cellular dna. | 1981 | 6277824 |
| herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication. | in addition to adenoviruses, which are capable of completely helping adenovirus-associated virus (aav) multiplication, only herpesviruses are known to provide any aav helper activity, but this activity has been thought to be partial (i.e., aav dna, rna, and protein syntheses are induced, but infectious particles are not assembled). in this study, however, we show that herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are in fact complete aav helpers and that aav type 2 (aav2) infectivity yi ... | 1981 | 6270377 |
| one functional copy of the long terminal repeat gene specifying the immediate-early polypeptide ie 110 suffices for a productive infection of human foetal lung cells by herpes simplex virus. | the hsv-1/hsv-2 intertypic recombinant bx1 (28-1) is heterotypic for the repeat sequences flanking the long unique region of the genome (irl and trl) and expresses both the immediate-early polypeptide ie 110 of hsv-1 and its functionally equivalent polypeptide ie 118 of hsv-2. the genome structures of five subclones of this recombinant and the immediate-early polypeptides they induce have been analysed. subclone 14 lacked most of the irl sequence, including the region from which part of the mrna ... | 1981 | 6271901 |
| differential immunologic reactivity and processing of glycoproteins ga and gb of herpes simplex virus types 1 and 2 made in vero and hep-2 cells. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) specify five major glycoproteins designated as ga, gb, gc, gd, and ge. previous studies have shown that ga and gb differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. moreover, ga and gb of hsv-1 crossreact in serologic tests with the corresponding glycoproteins of hsv-2. in this paper, we report on the reactivities of ga and gb of hsv-1 and hsv-2 with 24 independently de ... | 1981 | 6272294 |
| [use of a contraceptive suppository as chemoprophylaxis against sexually-transmitted diseases]. | the incidence of sexually transmitted diseases (std), especially gonorrhea, has risen dramatically in the past 2 decades. the population at greatest risk is also at high risk for unwanted pregnancies. since currently employed treatment methods are failing to curb the problem, a search was instituted for an available vaginal contraceptive that would have a prophylactic effect against these diseases. in vitro studies have indicated that a vaginal contraceptive made of phenylmercuric acetate is ... | 1981 | 6277015 |
| specific phosphorylation of e-5-(2-iodovinyl)-2'-deoxyuridine by herpes simplex virus-infected cells. | e-5-(2-iodovinyl)-2'-deoxyuridine (ivdurd), a highly selective and potent anti-herpes agent, is phosphorylated by primary rabbit kidney (prk) cells that have been infected with either herpes simplex virus type 1 (kos) or herpes simplex virus type 2 (g). however, ivdurd is not phosphorylated by mock-infected prk cells or prk cells that have been infected with a thymidine kinase-deficient mutant of herpes simplex virus type 1 (b 2006). these observations strengthen the concept that the selectivity ... | 1981 | 6263899 |
| mechanisms of expression of herpes simplex virus-common surface antigens in clonal cells of a herpes simplex virus type 2-transformed line. | rabbit antiserum hyperimmune to herpes simplex virus type 1 was used to study the expression of herpes simplex virus type-common surface antigens (csa) by indirect immunofluorescence tests in three representative cell clones isolated from a herpes simplex virus type 2-transformed hamster line, 155-4. these three clones showed different phenotypes with respect to csa expression: (i) a csa-positive type (clone (155-4-213), in which the antigens increased soon (5 h) after seeding at 37 degrees c, b ... | 1981 | 6264119 |
| herpes virus inactivation by chemical carcinogens: differential inactivation of herpes simplex viruses by 4-nitroquinoline 1-oxide and related compounds. | treatment of stocks of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) with the chemical carcinogen 4-nitroquinoline 1-oxide (nqo) resulted in inactivation of virus infectivity at rates which were directly dependent on the concentration of nqo and interval of exposure to nqo. hsv-1 strains were more sensitive than hsv-2 strains to inactivation by nqo, although survival curves of both hsv types were multicomponent. exposure of hsv-2 to a related group of chemicals suggested that the struct ... | 1981 | 6266824 |
| complement-fixing antibody to the ag-4 antigen in herpes simplex virus type 2-infected patients. | sera collected from confirmed herpes simplex virus type 2 (hsv-2) patients were found to be devoid of complement-fixing antibody to the ag-4 antigen at the time of the herpes lesion outbreak in 10 out of 13 cases. however, 1 to 4 weeks after hsv-2 lesion appearance, 28 out of 30 patients acquired complement-fixing antibody to the ag-4 antigen. the sera of these patients contained immunoglobulin m antibody activity and the ability to immunoprecipitate a 160,000-molecular weight early hsv-2 antige ... | 1981 | 6266964 |
| latency in vitro using irradiated herpes simplex virus. | human embryonic fibroblasts infected with u.v.-irradiated herpes simplex virus type 2 (hsv-2, strain 186) and maintained at 40.5 degrees c did not yield detectable virus. virus synthesis was induced by temperature shift-down to 36.5 degrees c. the induced virus grew very poorly and was inactivated very rapidly at 40.5 degrees c. non-irradiated virus failed to establish latency at 40.5 degrees c in infected cells. enhanced reactivation of hsv-2 was observed when latently infected cultures were su ... | 1981 | 6267167 |
| physical mapping of temperature-sensitive mutations of herpes simplex virus type 2 by marker rescue. | the physical mapping of six ts mutations of herpes simplex virus type 2 (hsv-2) is presented. the results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 hsv-1/hsv-2 intertypic recombinants. the order of these mutations on the physical map of hsv-2 is unambiguous and correlates almost exactly with the previously published genetic map of timbury & calder (1976). one of the mutants studied (hsv-2 ts12) has apparently two distinct conditionally lethal ts m ... | 1981 | 6267168 |
| experimental studies of acute and recurrent herpes simplex virus infections in the murine heart and dorsal root ganglia. | the multiplication of hsv-1 and hsv-2 strains in the heart and the corresponding dorsal root ganglia (drg) was examined in experimentally infected mice. infectious hsv-1 was recovered from the heart between the second and fourth day after inoculation and 3 days later from the drg. both the heart and drg yielded infectious hsv-2 from the fourth to the twenty-first day after inoculation. the hsv-2, but not the hsv-1, induced recrudescent disease in chronically infected mice up to 18 months after i ... | 1981 | 6267190 |
| [new antigens induced by herpes simplex virus in human tumors]. | tissues of malignant tumors of the genitalia (cervix, uterus body, ovary) contain a specific antigen associated with herpes simplex virus type 2 (hsv-2) identical with the antigen of the infected cells. the virus-induced antigen was detected in tissues of cervical carcinoma in 35% of cases, in 15% of tumors of the corpus uteri and in 13% of ovary tumors. hsv-2 was isolated from pathologically altered cervical carcinoma cells in 2 out of 56 cases examined. these facts indicate the presence of hsv ... | 1981 | 6267821 |
| kaposi's sarcoma. iv. detection of cmv dna, cmv rna and cmna in tumor biopsies. | in order to determine whether human cytomegalovirus- (cmv) dna homologous sequences as well as cmv-specific rna(s) and antigen(s) exist in tumor biopsies of kaposi's sarcoma (ks) dna-dna reassociation, rna-dna in situ cytohybridization and anticomplement immunofluorescence test (acif) tests were applied. three of 10 dnas extracted from kaposi sarcoma biopsies contained dna sequences homologous to radioactively labelled human cmv dna probe. the amount of cmv dna in these sarcoma tissues was calcu ... | 1981 | 6273333 |
| protectivity of herpes simplex virus antigens: studies in mice on the adjuvant effect of piclc and on the dependence of protection on t cell competence. | the efficacy of a herpes simplex virus type 1 (hsv-1) envelope antigen (eag) preparation against hsv infection was studied in t cell competent and t cell deficient mice. immuno-competent mice were successfully protected against herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a hsv-1 envelope antigen (hsv-1 eag) preparation. since hsv-1 eag was considerably less effective than the who ... | 1981 | 6268955 |
| differences in neurovirulence among isolates of herpes simplex virus types 1 and 2 in mice using four routes of infection. | differences in neurovirulence between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) were investigated using recent clinical isolates and laboratory-passaged strains in intravaginal, intranasal, intraperitoneal, and intracerebral infections of mice. the hsv-2 isolates caused higher death rates in all four infections. no differences in death rate were observed between recent and passaged isolates of either hsv-1 or hsv-2. after intravaginal inoculation, hsv-1 isolates replicated to higher ... | 1981 | 6273475 |
| antiherpesviral and anticellular effects of 1-beta-d-arabinofuranosyl-e-5-(2-halogenovinyl) uracils. | 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl) uracil (bv-ara-u) and 1-beta-d-arabinofuranosyl-e-5-(2-chlorovinyl)uracil (cv-ara-u) were tested for their anti-herpesviral activity in virus rating method, a plaque reduction method, and a virus yield reduction method, using human embryonic lung fibroblast (hel-f) cells, at a concentration as low as 0.1 microgram/ml, both drugs exerted a marked inhibitory effect on the development of cytopathogenic effect induced by herpes simplex virus type 1 (hsv-1 ... | 1981 | 6269482 |
| nonstructural proteins of herpes simplex virus. ii. major virus-specific dna-binding protein. | the major herpes simplex virus type 2 dna-binding infected cell-specific polypeptides 11 and 12 have been purified to homogeneity from extracts of virus-infected cells. monospecific antiserum to the purified protein has been made and used to examine virus temperature-sensitive mutants for defects in the synthesis of the protein and to probe virus dna synthesis in isolated chromatin. the purified protein acted directly on a polydeoxyadenylic acid-polydeoxythymidylic acid helix, reducing its melti ... | 1981 | 6270358 |
| induction of uterine cancer with inactivated herpes simplex virus, types 1 and 2. | a series of studies were performed to evaluate the oncogenic potential of inactivated herpes simplex viruses types 1 (hsv-1) and 2 (hsv-2) in the mouse cervix. hsv-1 or hsv-2 prepared in hep-2 cell cultures and inactivated by exposure to formalin or ultraviolet light was applied to the mouse cervix for periods ranging from 20 to 90 weeks. control mice were exposed for the same period to control fluids. vaginal cytologic preparations from all animals were examined weekly to detect epithelial abno ... | 1981 | 6269724 |
| molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection. | in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ... | 1981 | 6275122 |
| recovery of mice from herpes simplex virus type 2 hepatitis: adoptive transfer of recovery with immune spleen cells. | young balb/c mice inoculated intraperitoneally with herpes simplex virus type 2 develop focal necrotizing hepatitis. after infection, the livers of these mice show increasing virus titers, which reach a maximum on day 3 after infection; this is followed by a dramatic decrease in the amount of virus recovered on days 4 and 5. this decrease in virus content is accompanied by a progressive infiltration of the lesions with mononuclear leukocytes and an apparent resolution of the lesions. adoptive tr ... | 1981 | 6269998 |
| conversion of herpetic lesions to malignancy by ultraviolet exposure and promoter application. | many lines of evidence exist associating herpes simplex virus (hsv) with the development of carcinoma, but much of this evidence is anecdotal or associative in nature and does not prove a cause and effect. the purpose of this research was to investigate the oncogenic potential of hsv type 2 (hsv-2) in vivo. a mouse model for lip carcinogenesis was designed to combine hsv-2 infection, u.v. exposure and tetradecanoyl-phorbol-acetate (tpa) application. hsv-2 inoculation on to abraded mouse lips was ... | 1981 | 6270265 |
| microscopic examination of the intracellular fate of infectious herpes simplex virus dna. | the intracellular fate of 3h-labeled exogenous herpes simplex virus type 2 dna following the exposure of recipient cells to selected dna facilitators and the ultrastructural changes which ensued in transfected cultures were examined. in most cases, electron microscopic radioautography demonstrated that exogenous dna reached the nucleus within 1 h postinoculation. at later time periods, enlargement of nuclei and margination of chromatin were noted. cells treated with polyornithine differed signif ... | 1981 | 6276330 |
| herpesvirus-induced antigens in squamous-cell carcinoma in situ of the vulva. | antigens induced by herpes simplex virus type 2 (hsv2) were found to be associated with squamous-cell carcinoma in situ of the vulva in nine of 10 patients. the hsv2-induced antigens are dna-binding proteins that are normally present in the nuclei of infected cells, but in the cells of the carcinomas in situ they were found in the cytoplasm. whole-virion structural antigens were not present, although there was serologic evidence of previous hsv2 infection in patients tested for the presence of a ... | 1981 | 6265780 |
| the influence of different modes of immunization on the experimental genital herpes simplex virus infection of mice. | previous investigations, which simulated the usual sequence of the human herpes simplex virus (hsv) infections, had shown that the oral infection of mice with hsv-1 caused only weak protection from genital infection with hsv-2, although the course of infection was attenuated and lethality diminished. this heterologous, heterotopic model was compared with a homologous, heterotopic and a heterologous, homotopic model. the results did not differ very much, although the homologous immunization prote ... | 1981 | 6268958 |
| identification of a virus-specific polypeptide associated with a transforming fragment (bglii-n) of herpes simplex virus type 2 dna. | the bglii n fragment of herpes simplex virus type 2 (hsv-2) dna (approximately 0.58 to 0.63 map unit) was examined for encoded products. using plasmid pgz59, which consists of bglii-n cloned in pat153, in conjunction with hybrid arrested translation, mrna selection, and in vitro protein synthesis, we found that the major translated product of this region has an approximate molecular weight of 37,800. by further mapping, coding sequences for this polypeptide were located within the region of bgli ... | 1981 | 6270369 |
| phospholipid synthesis in human embryo fibroblasts infected with herpes simplex virus type 2. | the effect of herpes simplex virus type 2 infection on the synthesis of phospholipids in human embryo fibroblasts was determined at temperatures permissive (35 c) or nonpermissive (42 c) for virus replication. incorporation of [32p]i was decreased by herpes simplex virus type 2 infection after 6 hr, which corresponds to the time of initiation of progeny virus production. no differences were observed in the relative incorporation of [32p]i phospholipid classes. in another series of experiments ce ... | 1981 | 6270493 |
| in vitro studies of a co-carcinogenic effect of vaccinia and herpes group viruses. | the results of studies of a co-carcinogenic effect of two human infectious viruses in tissue culture are reported here. viable vaccinia virus actively replicating in the cells of primary balb/c tissue culture and in a number of continuous murine cell lines has been shown to induce in them expression of major structural p30 protein of murine retroviruses. vaccinia virus has been also shown to cause biochemical transformation of murine cells. evidence for the capacity of herpes simplex virus type ... | 1981 | 6270580 |
| herpes simplex virus type 2 and cervical cancer. | 1981 | 6271502 | |
| physical mapping of drug resistance mutations defines an active center of the herpes simplex virus dna polymerase enzyme. | the genome structures of herpes simplex virus type 1 (hsv-1)/hsv-2 intertypic recombinants have been previously determined by restriction endonuclease analysis, and these recombinants and their parental strains have been employed to demonstrate that mutations within the hsv dna polymerase locus induce an altered hsv dna polymerase activity, exhibiting resistance to three inhibitors of dna polymerase. the viral dna polymerases induced by two recombinants and their parental strains were purified a ... | 1981 | 6270349 |
| characterization of rat embryo cells transformed by ts mutants and sheared dna of herpes simplex virus types 1 and 2 and a derived tumor cell line. | four independent isolated lines transformed by ts mutant or sheared dna of herpes simplex virus types 1 and 2 and one tumor cell line were characterized according to their behavior in assays of aggregation, growth in low serum, doubling times, saturation density, agglutination by lectins, uptake of metabolites, and production of plasminogen activator. the criteria used to select the transformed cells used in this study were the formation of altered foci of cells followed by the formation of a co ... | 1981 | 6263467 |
| induction of natural killer cells by herpes-simplex virus type 2 in resistant and sensitive inbred mouse strains. | infection of mice with herpes simplex virus type 2 (hsv 2) stimulated natural killer (nk) cells in a variety of inbred mouse strains including athymic nude mice. essentially all mouse strains tested exhibited high nk activity on day four after virus inoculation. assayed 24 hours after infection, swr/j, akr/j, sjl/j and c57b1/10j mice were low or negative for these non-virus-specific cytotoxic responses. whereas the first two mouse strains were most sensitive to the lethal effects of hsv 2, the l ... | 1981 | 6263793 |
| nucleotide sequences of the joint between the l and s segments of herpes simplex virus types 1 and 2. | the a sequence of herpes simplex virus (hsv) is present as a direct repeat at the genomic termini and also in inverted orientation at the joint between the l and s segments. dna sequences have been determined for the joint regions of the genomes of hsv-1 and hsv-2, and relative to these sequences the genomic termini are in both cases located close to a short direct repeat of 17 to 21 base pairs (bp) at the b-a and a-c junctions. the hsv-1 joint region contains three separate tandem direct reiter ... | 1981 | 6270266 |