Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| type-common cp-1 antigen of herpes simplex virus is associated with a 59,000-molecular-weight envelope glycoprotein. | the cp-1 antigen of herpes simplex virus type 1 (hsv-1) is a glycoprotein found in the soluble portion of infected cells, in detergent extracts of infected cell membranes, and in the envelope of purified virus. antisera were prepared against a further purified form of cp-1 prepared from hsv soluble antigen mix; a glycoprotein, gp52, isolated from detergent-treated infected cells; and detergent extracts of purified virus. each of the antisera reacted with cp-1 to give a single immunoprecipitin ba ... | 1978 | 80458 |
| antibody-dependent cell-mediated cytotoxicity to herpes simplex virus type 2 infected target cells in the course of cervical carcinoma. | the antibody-dependent cell-mediated cytotoxicity (adcc) assay was used as a sensitive instrument to study if specific antibodies acting synergistically with lymphocytes to induce target cell destruction, could be detected in sera from patients with cervical carcinoma. significant cytotoxicity to herpes simplex type 2 (hsv-2) infected target cells was observed in sera of 17 out of 23 (74%) surviving patients with cervical cancer who were followed with at least two serum samples. a rise in cytoly ... | 1978 | 101081 |
| detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the papanicolaou stain. | the sensitive peroxidase-antiperoxidase (pxapx) method individually and in conjunction with the papanicolaou (pap) stain was used to detect herpes simplex virus (hsv) in specimens from human female genitalia. initial studies using a model system of hsv-1 or hsv-2-infected vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diamino ... | 1979 | 94600 |
| iontophoretic application of adenine arabinoside monophosphate for the treatment of herpes simplex virus type 2 skin infections in hairless mice. | 1979 | 94335 | |
| herpes simplex virus type 2 and carcinoma of urinary bladder. | 1979 | 94309 | |
| detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay. | a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ... | 1979 | 94044 |
| a rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology. | a rapid simplified technique, based on restriction-endonuclease analysis of radioactively labelled d.n.a. is described; it distinguishes herpes-simplex virus type 1 strains from herpes-simplex virus type 2 unambiguously. large numbers of isolates can be screened in 4--5 days. | 1979 | 86095 |
| microfluorometric quantitation of size, deoxyribonucleic acid and viral antigenic determinants in cells productively infected with hsv-2. | the interaction of herpes simplex virus type 2 (hsv-2) with the eukaryotic human cell line (hep-2) was investigated by flow microfluorometric analysis. the three parameters that were quantitated include cell size, deoxyribonucleic acid content and the expression of virus-specific antigens. productively infected cells are always smaller than uninfected ones, and they resolve into two populations with respect to viral antigenic content. consistent with viral replication, one of these two populatio ... | 1979 | 94525 |
| immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture. | proteins released from herpes simplex virus type 1 (hsv-1)-and type 2 (hsv-2)-infected hep-2 cells have been characterized by the crossed immunoelectrophoretic technique. both hsv type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. antigen ag-6, an hsv-1-specific antigen, was found in high concentration in the medium as compared to other hsv antigens released from hsv-1-infected cells. the hsv-2-specific antigens, ag-4 and ag-1, were released in m ... | 1979 | 85610 |
| production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. | we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ... | 1979 | 91687 |
| [herpes simplex virus type 2 antibodies in the husbans of women with cervical cancer]. | 1979 | 228506 | |
| alterations in glycosphingolipid patterns in a line of african green monkey kidney cells infected with herpesvirus. | the major glycosphingolipids (gsls) of a line of african green monkey kidney cells (bgm) were characterized as glucosylceramide, lactosylceramide, galactosyl-galactosyl-glucosylceramide, and n-acetylgalactosaminyl-galactosyl-galactosyl-glucosylceramide. neutral gsls accounted for approximately 80% of the total gsls isolated. the predominant gangliosides were n-acetylneuraminyl-galactosyl-glucosylceramide, n-acetylgalactosaminyl-n-acetylneuraminyl-galactosyl- glucosylceramide, and galactosyl-n-ac ... | 1979 | 229262 |
| host-cell response to herpes virus infection in central and peripheral nervous tissue in vitro. | in an organotypic nerve cell culture system, all cells in both the central and the peripheral nervous system (cns, pns) components supported replication of herpes simplex virus types 1 and 2 (hsv 1, hsv 2). in hsv 1 infection, cellular response was particularly characterized by the formation of small syncytia (which involved neurons) and by the presence of bundles of interwoven fine filaments within the nuclei of infected cells. in hsv 2 infection, groups of parallel tubules characteristically f ... | 1979 | 231090 |
| blocking of acid-fixed nuclear binding of epstein-barr virus nuclear antigen (ebna) by different dna species. | treatment of epstein-barr virus-determined nuclear antigen (ebna) with dna resulted in blocking of its ability to convert acid-fixed ebna-negative cell nuclei to an ebna-positive form. epstein-barr virus (ebv) dna, herpes simplex virus type 2 (hsv-2) dna and dna isolated from three lymphoblastoid cell lines differed in their potency to block this reaction. ebv dna was found to be about three times more effective than cellular dnas in abolishing the ability of dna-cellulose-purified ebna to conve ... | 1979 | 231093 |
| mutant of herpes simplex virus type 2 with temperature-sensitive lesions affecting virion thermostability and dnase activity: identification of the lethal mutation and physical mapping of the nuc-lesion. | we had previously shown that a temperature-sensitive (ts) mutant of herpes simplex virus type 2 strain hg52, ts13, induced a heat-labile dnase activity in infected cells (b. francke, h. moss, m. c. timbury, and j. hay, j. virol. 26:209-213, 1978). earlier work indicated that the mutant also possessed temperature-sensitive infectivity (i. w. halliburton and m. c. timbury, j. gen. virol. 30:207-221, 1976). in this study temperature-stable revertants of ts13 have been isolated; examination of them ... | 1979 | 232166 |
| effect of trisodium phosphonoformate in genital infection of female guinea pigs with herpes simplex virus type 2. | a genital herpesvirus type 2 infection in guinea pigs has been used to evaluate the inhibitory effect of phosphonoformate on the infection. an early topical treatment prevented the appearance of vesicles and histopathological changes and no virus could be recovered. when treatment, either topical or both systemic and topical, was delayed to 24 hours post infection no therapeutic effect was observed. | 1979 | 508093 |
| effects of cannabinoids on host resistance to listeria monocytogenes and herpes simplex virus. | previous investigations from our laboratories have demonstrated that cannabinoids possess immunosuppressive properties. the present studies were designed to determine whether these agents decrease host resistance to infections with listeria monocytogenes and herpes simplex virus type 2. host resistance was measured by changes in the 50% lethal dose of the pathogen in cannabinoid-treated and control mice. the effect of cannabinoids on resistance to l. monocytogens was dose dependent. delta-9-tetr ... | 1979 | 313368 |
| effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2. | treatment of vero cells infected with herpes simplex virus type 2 with the proper concentrations of hydroxyurea (hu) reduced the production of infectious virus and markedly increased the accumulation of tubular structures in the nuclei when the drug was added within 6 hours after infection. similar accumulation of tubular structures in the infected nuclei was also observed when infected cells were treated with phosphonoacetic acid at proper concentrations. the release of hu-treated, herpes simpl ... | 1979 | 229802 |
| isolation of the major herpes simplex virus type 1 (hsv-1)-specific glycoprotein by hydroxylapatite chromatography and its use in enzyme-linked immunosorbent assay for titration of human hsv-1-specific antibodies. | a 131,000 molecular weight herpes simplex virus type 1 (hsv-1) glycoprotein designated antigen number 6 (ag-6) was previously shown to possess almost exclusively hsv-1-specific antigenic sites. fused rocket and crossed immunoelectrophoresis of fractions obtained from hydroxylapatite chromatography of crude hsv-1 antigen (triton x-100-solubilized, infected tissue culture cells) showed that a subfraction of ag-6 could be separated from the other hsv antigens. enzyme-linked immunosorbent assay with ... | 1979 | 230199 |
| tumorgenicity of herpesvirus hominis type 2-transformed cells (line 333-8-9) in adult hamsters. | inoculation of adult hamsters with greater than 1.5 x 10(2) herpesvirus type 2 (hsv-2) transformed hamster embryo fibroblasts induced rapidly growing tumours at the site of inoculation, in more than 50 per cent of animals. the tumours were examined by light and electron microscopy, and were histologically anaplastic fibrosarcomas. lung metastases were frequently found in tumour-bearing animals; these were usually only seen microscopically, and were cytologically similar to the primary tumours. t ... | 1979 | 231649 |
| necrotising balanitis due to a generalised primary infection with herpes simplex virus type 2. | virological studies have proved herpes simplex virus type 2 to be the cause of the severe primary infection in a 37-year-old man with necrotising balanitis. symptoms of urethritis preceded the appearance of a severe local lesion; dissemination of infection occurred and lesions developed on the skin of the scalp, neck, trunk, buttocks, arms, legs, and feet. no evidence of involvement of the central nervous system was found, and the penile and skin lesions improved rapidly after the application of ... | 1979 | 427514 |
| [modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus]. | the influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (hsv-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-mca), was studied. solid tumors developed in 20-mca-treated mice on the side of the carcinogen administration within 81--89 days. the simultaneous administration of 70 intracerebral ld50 of hsv-2 subcutaneously into a hind leg pad and 0.1 mg 20-mca subcutaneously into the inguinal area was accompanied by a syne ... | 1979 | 462927 |
| [seroepidemiological study of herpes simplex virus type 2 in adult women of costa rica]. | the frequency of genital herpes simplex type 2 infections in a group of twenty adult costa rican women was studied by isolation of the virus and the measurement of neutralizing antibody activity in sera. the virus could not be isolated in any of the vaginal secretions. neutralizing antibody activity to herpes virus types 1 and 2 was found in sera from sixteen subjects. an antibody ii/i index equal to or larger than 87, indicative of infection with herpes simplex type 2 was found in fifty per cen ... | 1979 | 233038 |
| the presence of herpes simplex virus dna in hamster embryo cells transformed by a temperature sensitive mutant, ts-4. | hamster embryo cells transformed with a temperature sensitive mutant of hsv-2, ts-4t, were examined for the presence of viral genomes. dna-dna reassociation kinetics showed that only a small portion (11 +/- 4%) of hsv-2 was present in the transformed cells. | 1979 | 231439 |
| comparative study of herpes group virus-induced dna polymerases. | a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ... | 1979 | 231586 |
| presence of herpes simplex virus type 2 dna in hamster cells oncogenically transformed by ultraviolet-inactivated virus. | herpes simplex virus type 2 (hsv-2) dna has been detected by molecular hybridization in hamster fibroblast cells oncogenically transformed by ultraviolet-irradiated virus. at early passages after cloning in soft agar, about 40% of the hsv-2 genome was present in all the transformed cell lines at one to six copies per cell. in cell lines derived from tumors induced by these cells, the same percentage of the hsv-2 genome was also found with more uniform number of copies (between two and three). th ... | 1979 | 224988 |
| serological studies on the antigenic relationship between herpes simplex virus and varicella-zoster virus. | if crossreacting antibodies between varicella-zoster virus (vzv) and herpes simplex virus (hsv) exist, one would expect more positive reactions with vzv in a group of hsv positive patients than in a group of hsv negative patients. this statement can only apply to a group of individuals where positive and negative reactions with respect to hsv and vzv are evenly distributed. such a distribution can only be found among children. therefore, the relationship between hsv-1 and vzv was the only one wh ... | 1979 | 232073 |
| phosphorylation of a ribosomal protein and of virus-specific proteins in cells infected with herpes simplex virus. | in cells infected with herpes simplex virus a protein associated with the small subunit of ribosomes became phosphorylated. it was not detectably labelled with 14c-amino acids added after infection and is therefore probably a cellular protein. the phosphorylated ribosomal proteins from hsv-1- and hsv-2-infected cells were indistinguishable electrophoretically and had an apparent mol. wt. of about 48 000. phosphorylation of the 48k protein was detected 2 to 3 h after infection and reached a maxim ... | 1979 | 232130 |
| virus-specific dna sequences present in cells which carry the herpes simplex virus thymidine kinase gene. | two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of hsv-2-specific dna sequences. both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the hsv-2 genome. revertant cells, which contained no detectable thymidine kinase, did not contain this dna sequence. the failure of ecor1-restricted hsv-2 dna to act as a donor of the thymidine kinase gene in transformation ex ... | 1979 | 232133 |
| life-threatening pharyngitis caused by herpes simplex virus, type 2. | a 21-year-old man who had pharyngitis needed hospitalization for observation of airway patency and for parenteral fluid therapy. infection with herpes simplex virus, type 2 (hsv-2), was diagnosed on the basis of the finding of significantly increased igm indirect hemagglutination antibody titers. the pharyngitis appeared to have been contracted by heterosexual orogenital contact (cunnilingus). the results of this case study indicate that hsv-2 infection should be considered in the differential d ... | 1979 | 221994 |
| experimental encephalitis in the newborn rat due to herpes simplex virus type 2. | 1979 | 222606 | |
| reactivation of herpes simplex virus type 2 from a quiescent state by human cytomegalovirus. | the ability of human cytomegalovirus to stimulate replication of herpes simplex virus type 2 (hsv-2) was examined. the system used involved hsv-2-infected human embryonic lung cells under conditions (39.5-40 degrees c) in which hsv-2 remains undetectable. reactivation of hsv-2 was maximal and persisted for the longest duration when cultures were superinfected with 0.02 plaque-forming unit of human cytomegalovirus per cell. infectious hsv-2 appeared 2 days after superinfection with human cytomega ... | 1979 | 230494 |
| growth inhibition by acycloguanosine of herpesviruses isolated from human infections. | inhibition by acycloguanosine (acg) of plaque formation by harpes simplex virus types 1 and 2 (hsv-1 and hsv-2), varicella-zoster virus, and cytomegalovirus was studied. seventeen clinical isolates of hsv-1 were inhibited by acg at a mean 50% inhibitory dose (id(50)) of 0.15 +/- 0.09 mum. the mean id(50) for 10 isolates of hsv-2 was 1.62 +/- 0.76 mum, and for four isolates of varicella-zoster virus it was 3.75 +/- 1.30 mum. the id(50)'s for two cytomegalovirus isolates were 100 and 160 mum, and ... | 1979 | 230783 |
| [epidemiology of carcinoma of the cervix (author's transl)]. | in order to explain why modes of sexual life influence the incidence of cervical carcinoma, several factors have been claimed to be involved. there is no solid basis for the hypothesis that sperm may be mutagenic. by contrast, epidemiological studies showed that partners whose life is not sedentary bring back home to their wives an increased risk of cervical carcinoma. the infectious factor does not seem to be associated with syphilis but may well be represented by herpes genitalis virus (herpes ... | 1979 | 230875 |
| stimulation of human cell dna synthesis by defective herpes simplex virus type 2. | 1979 | 228477 | |
| herpes virus infection as a cofactor in carcinogenesis: supernatants of herpes virus type 1- and type 2-infected cell cultures contain a cell growth-stimulating factor. | some cell cultures synthesize a mitogenic factor (dnasf) following their lytic infection with either hsv-1 or hsv-2 which is shed into the medium. of six cell lines permissive to hsv infection tested, three produce dnasf (cv1, hf, mca) and a significantly higher (3h)-tdr incorporation was obtained with indicator cells growing in supernatants of virus-infected cells (supv+) than in the corresponding supernatants of sham-treated cells (supv-). sometimes the values obtained with supv+ exceed those ... | 1979 | 223098 |
| virucidal activity of retinal. | herpes simplex virus type 2 and simian virus 40 were rapidly inactivated by retinal at micromolar concentrations. other fat-soluble vitamins, particularly vitamin a derivatives, were also active against herpes simplex virus type 2 and several lipid-containing bacteriophages. | 1979 | 228596 |
| some aspects of the role of viruses in cancer. | the cells of tumours induced by many oncogenic dna viruses, or cells transformed in vitro, contain virus-specific t and transplantation antigens; these have been described for sv40 virus, polyoma virus and adenoviruses. the investigation of viruses as causes of malignant disease in man has sought to establish whether tumour cells possess these virus-specific proteins; however, to date and with the limitations of present techniques, this enquiry has not demonstrated the above viruses as causal of ... | 1979 | 223141 |
| regulation of persistent infection with herpes simplex virus in vitro by hydrocortisone. | about 1% of raji cells showed sensitivity to herpes simplex virus type 2 (hsv-2) infection when tested by infectious center assays or immunofluorescence tests, and the percentage did not change during cell passage. the addition of hydrocortisone to raji cells persistently infected with hsv-2 caused a marked increase in virus production and in the number of hsv-producing cells. in the case of hsv-1, it was shown that the addition of hydrocortisone was required to maintain persistent infection. th ... | 1979 | 229255 |
| association of herpes simplex virus (hsv) with cervical cancer by lymphocyte reactivity with hsv-1 and hsv-2 antigens. | twenty-six women with invasive cervical cancer were examined for lymphocyte responsiveness to phytohemagglutinin (pha) herpes simplex virus (hsv). blood specimens were obtained from each patient before radiation therapy and separated into subpopulations by ficoll-hypaque centrifugation. lymphocytes were cultured in rpmi-1640 containing autologous plasma and exposed to pha, hsv-1, hsv-2, and control antigens. the results compared with those of women having negative papanicolaou smears and matched ... | 1979 | 223438 |
| the influence of cell type on the production of filaments by herpes simplex virus type 2. | 1979 | 228892 | |
| demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral dna. | 1979 | 227967 | |
| storage and transport of cultures for herpes simplex virus, type 2. | tryptic soy broth and brain-heart infusion broth maintained herpes simplex virus within +/- 1 serial dilution of the original titer in all of 51 samples held for one to three days at 4 c. in contrast, holding temperatures of -20 c or 22 c resulted in losses of titer of 10(2) or more. holding periods as long as five days prior to inoculation did not delay the appearance of the cytopathic effect following inoculation. unmodified tryptic soy broth or brain-heart infusion broth and holding periods a ... | 1979 | 229726 |
| physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue. | mutations (paar) in herpes simplex virus (hsv) which confer resistance to phosphonoacetic acid involve genes associated with virus-induced dna polymerase activity. two mutants of hsv (hsv-1 tsh and hsv-2 ts6) produce a thermolabile dna polymerase activity. in this study, the ts lesions present in these mutants and those present in two independent phosphonoacetic acid-resistant mutants of hsv-1 and hsv-2 (paar-1 and paar-2) have been physically mapped by restriction endonuclease analysis of recom ... | 1979 | 225553 |
| preliminary studies of the mode of action of arildone, a novel antiviral agent. | arildone (also known as win 38020), a novel aryl diketone, inhibited replication of herpes simplex virus type 2 in tissue culture by interfering with an event that occurs prior to 6 h postinfection. the inhibition could be partially reversed by washing. although the exact mechanism of action is unknown, neither viral deoxyribonucleic acid nor viral proteins were synthesized in the presence of arildone. | 1979 | 224808 |
| activities of 5,6-dihydro-5-azathymidine against herpes simplex virus infections in mice. | 5,6-dihydro-5-azathymidine (dhadt), a nucleoside antibiotic inhibitory for herpes simplex virus (hsv) in cell cultures (h. e. renis, antimicrob. agents chemother. 13: 613-617, 1978), was evaluated in mice with experimental hsv infections. dhadt protected mice infected with hsv type 1 (hsv-1) when the virus was inoculated intravenously and the drug was given by subcutaneous or oral routes. the activity observed was dependent on the dose and schedule of treatment. doses of 100 to 400 mg/kg given t ... | 1979 | 426515 |
| neoplastic transformation of cultured syrian hamster embryo cells by dna of herpes simplex virus type 2. | syrian hamster embryo cells were transformed to a neoplastic phenotype after exposure to herpes simplex virus type 2 (s-1) dna at concentrations (less than or equal to 0.01 microgram per 60-mm dish) at which infectivity was no longer demonstrable. transformed cells manifested in vitro phenotypic properties characteristic of the neoplastic state, expressed herpes simplex virus-specific antigens, and induced invasive tumors in vivo. transfection and transformation of syrian hamster embryo cells wi ... | 1979 | 225525 |
| immunotherapy and drugs in neonatal disseminated herpes simplex virus type 2 infections: a mouse model. | 1979 | 226512 | |
| effect of pyran on latency after herpes simplex virus infections. | the immunomodulator pyran protected mice against both herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) infections. in infections of the lip with hsv-1, prophylactic administration of pyran reduced the severity of the herpetic lesions and enhanced their resolution, but did not decrease the high incidence of development of latent hsv-1 infection of the trigeminal ganglia. in vaginal infections with hsv-2, prophylactic administration of pyran either systemically or locally reduced mortality, ... | 1979 | 464586 |
| appearance of rosette-forming macrophages in the lungs of influenza virus-infected mice. | approximately one fifth of the macrophages obtained from the lungs of mice infected 2 to 5 days with influenza a/hk virus were found to rosette well with either unmodified human, chicken, or guinea pig erythrocytes, but not with erythrocytes from hamsters, sheep, or mice. rosette-forming macrophages were seldom seen in suspensions from uninfected mice (3+/-3%) or mice infected 24 h previously (3+/-3%). rosette formation was not due to virus hemadsorption, as indicated by the failure of specific ... | 1979 | 500196 |
| differential and selective inhibition of cellular and herpes simplex virus dna synthesis by arabinofuranosyladenine. | the influence of 9-beta-d-arabinofuranosyladenine (beta araado) and of its anomer 9-alpha-d-arabinofuranosyladenine (alpha araado) was studied in non-infected cells and cells infected with herpes simplex virus type 1 (hsv-1) and hsv type 2 (hsv-2). alpha araado is a strong inhibitor of proliferation of non-infected cells. multiplication of hsv-1 and hsv-2 is not affected at all by alpha araado, while their growth is strongly inhibited by beta araado. alpha araado exerts no effect on the incorpor ... | 1979 | 227462 |
| haemorrhage in neonatal hepatic necrosis due to herpes infection. | a case of early neonatal severe bleeding and persistent hypoglycemia with a fatal outcome is reported. the autopsy examination revealed the features of neonatal hepatic necrosis. further study by the electron microscopy indicated the presence of herpes type particles in the nucleus and cytoplasm of the remaining liver cells. serological study of the maternal blood, post partum, revelaed positive reaction to herpes simplex virus type 2 at low titer. it is believed that intrauterine herpes infecti ... | 1979 | 515805 |
| herpes simplex type 2 encephalitis concurrent with known cerebral metastases. | a 62-year-old woman developed neurologic deficits 7 months after pulmonary lobectomy for alveolar cell carcinoma of the lung. ct scan of the head demonstrated two metastases with marked peritumoral edema. administration of decadron, chemotherapy and 3,000 rad cranial radiation resulted in dramatic improvement of dysphasia and right hand paresis. almost 2 months later, rhythmic, involuntary movements of the left hand developed. there was progression to multifocal seizures, grand mal seizures, pos ... | 1979 | 225922 |
| production of tubular structures in vero cells infected with herpes simplex virus type 2; effects of ultraviolet light irradiation and antimetabolites. | in order to investigate the nature of tubular structures specifically found in herpes simplex virus type 2 (hsv-2)-infected cells, the multiplication of hsv-2 was studied in vero cells cultured in the presence of varying concentrations of cytosine arabinoside (ara-c) and cycloheximide (ch), inhibitors of dna synthesis and protein synthesis respectively. ara-c, at a concentration of 60 micrograms/ml, inhibited the multiplication of hsv-2 by more than 99% and also prevented the appearance of tubul ... | 1979 | 228001 |
| dnase induced after infection of kb cells by herpes simplex virus type 1 or type 2. ii. characterization of an associated endonuclease activity. | purified preparations of the "exonuclease" specified by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) possess an endonuclease activity. the exonuclease and endonuclease activities copurify and cosediment in a sucrose density gradient. endonuclease activity is only observed in the presence of a divalent cation, and mg(2+) or mn(2+) is equally effective as a cofactor with an optimal concentration of 2 mm. a slight amount of endonuclease activity is observed in the presence of ca(2+), wher ... | 1979 | 228069 |
| persistence of herpes simplex virus types 1 and 2 in infected individuals. | during a 23-month period, 18 patients with facial or genital herpetic lesions were examined; culture specimens from each patient were obtained three times per week for virologic studies. the isolated viruses were identified, and the average duration of herpesvirus in lesions was determined. herpes simplex virus type 1 (hsv-1) was isolated from facial lesions for a mean duration of 3 1/2 days. in contrast, herpes simplex virus type 2 (hsv-2) was isolated from genital lesions for a mean duration o ... | 1979 | 219784 |
| characterization of human tk- cell lines transformed to a tk+ phenotype by herpes simplex virus type 2 dna. | human tk- cells carrying the hsv-2 tk gene as a result of transformation with virus dna express a tk activity of virus origin and maintain the tk+ phenotype when grown in hat medium. under non-selective conditions, however, reversion to a tk- phenotype occurs with a significant frequency characteristic of each transformed line. once reversion has occurred the tk- phenotype appears to be stable, since only very rare instances of tk- to tk+ reversion have been observed. tk- revertants were suscept ... | 1979 | 215706 |
| herpes simplex virus isolation from pyoderma gangrenosum lesions in a patient with chronic lymphatic leukemia. | herpes simplex virus type 2 was isolated and identified from the vesicular border of pyoderma gangrenosum lesions on the genital region of a patient with chronic lymphatic leukemia. dramatic relief of pain as well as quick disappearance of the vesicular margin of the lesions and of the inflammatory halo around them occurred as a result of local treatment with a solution of zinc acetate. a careful search for a viral agent should be done in every case of pyoderma gangrenosum occurring in a patient ... | 1979 | 220109 |
| thin-layer immunoassay for determination of antibodies to herpes simplex virus. | thin-layer immunoassay (tia) is a simple serological technique suitable for analysis of large numbers of samples. in this study, tia was evaluated for determination of antibodies to herpes simplex virus. herpes simplex virus antigen used in tia was purified from material released from virus-infected cells. the results obtained by tia were compared with those obtained by neutralization and complement fixation tests. tia was found to be as sensitive as the neutralization test for demonstration of ... | 1979 | 222798 |
| evaluation of indirect hemagglutination and its inhibition in the differentiation between antibodies to herpes simplex virus types 1 and 2 for seroepidemiologic studies: use of a ii/i index threshold of 85 and an assay of type-specific antibodies. | two parameters, a ii/i index threshold value of 85 and an assay of type-specific antibodies to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), were assessed, for seroepidemiologic purposes, for their efficacy in the establishment of past infections with hsv-1 and hsv-2 as indicated by the results of tests for indirect hemagglutination and its inhibition. neither criterion alone established past infection with either hsv-1 or hsv-2. however, by combination of the two parameters, antibodie ... | 1979 | 220339 |
| replication of herpes simplex virus type 2 in normal dysplastic and neoplastic human cervical epithelia. | 1979 | 221223 | |
| [comparative study of the effectiveness of the purification and concentration of herpes simplex virus types 1 and 2]. | the results of comparative studies on concentration and purification of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) by ficol density gradient centrifugation are presented. a two-phase distribution of extracellular hsv was established in phycol density gradient centrifugation: in zones with density of 1.110-1.114 and 1.088-1.085 g/ml. the effectiveness of purification of hsv preparations recovered from the corresponding gradient zones was determined by electron microscopy and quantit ... | 1979 | 219611 |
| lymphocyte transformation for the detection of herpesvirus-induced tumor-associated antigens. | cell-mediated immunity to fibroblasts transformed by herpes simplex virus type 2 was investigated with a lymphocyte assay system. the assay system was first standardized with phytohemagglutinin, a nonspecific stimulator of blastogenesis. hamster splenic and blood lymphocytes reacted to phytohemagglutinin with a dose-response curve similar to that reported for other rodent species. splenic lymphocytes from hamsters bearing isografts, induced by herpes simplex virus type 2, were transformed by cel ... | 1979 | 225013 |
| phosphorylation of arabinofuranosylthymine in non-infected and herpesvirus (tk+ and tk-)-infected cells. | the phosphorylation of arabinofuranosylthymine (arathd) has been studied both in non-infected cells and in those infected with herpes simplex virus (hsv-1, lennette; hsv-1, ies and hsv-2, d-316). in these experiments, hsv strains were used which either contain (lennette, tk+ and d-316 tk+) or lack (ies, tk-) the capacity to induce pyrimidine deoxyribonucleoside kinase. it was found that extracellularly administered arathd is phosphorylated to ara ttp via aratmp and aratdp in both non-infected an ... | 1979 | 225422 |
| herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. 2. electron microscopic observations of myelin degeneration. | organotypic cultures of mouse spinal cord with attached dorsal root ganglia, which contain both central and peripheral myelin in the one unit of tissue, were infected with hsv 1 or hsv 2 and studied using electron microscopy. intranuclear viral nucleocapsids and intracytoplasmic enveloped particles were found in the schwann cells associated with peripheral myelin and in oligodendroglia associated with central myelin. degeneration of peripheral myelin most commonly involved an asymmetrical swelli ... | 1979 | 219153 |
| herpes simplex virus type 2 functions expressed during stimulation of human cell dna synthesis. | experiments were designed to identify herpes simplex virus type 2 (hsv-2)-specific functions expressed during stimulation of human embryo fibroblast dna synthesis. cultures were partially arrested in dna synthesis by pretreatment with 5-fluorouracil and maintenance in low-serum (0.2%) medium during virus infection. results showed that continuous [methyl-(3)h]thymidine uptake into cellular dna was ninefold greater in hsv-2-infected than in mock-infected cultures measured after 24 h of incubation ... | 1979 | 219236 |
| stimulation of human cellular thymidine kinase activity during abortive infection with herpes simplex virus type 2. | 1979 | 223308 | |
| molecular genetics of herpes simplex virus. ii. mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells. | we have mapped the location in herpes simplex virus (hsv) dna of (i) three mutations at different loci (syn loci) which alter the social behavior of infected cells from clumping of rounded cells to polykaryocytosis, (ii) a mutation which determines the accumulation of one major glycoprotein [vp8.0(c(2))], and (iii) the sequences encoding four major virus glycoproteins [vp8.0(c(2)), vp7(b(2)), vp8.5(a), and vp19e(d(2))]. the experimental design and results were as follows. (i) analysis of hsv-1 x ... | 1979 | 219254 |
| sites of integration of herpes simplex virus type-2 thymidine kinase gene in human chromosomes. | 1979 | 223994 | |
| anatomy of herpes simplex virus dna. xi. apparent clustering of functions effecting rapid inhibition of host dna and protein synthesis. | herpes simplex virus type 2 (hsv-2) strains inhibit the synthesis of both dna and protein of the host cell more rapidly than hsv-1 strains. several intertypic hsv-1 x hsv-2 recombinants and parental strains were examined for their ability to inhibit rapidly the synthesis of host protein and dna. the two functions cosegregated in all of eight recombinants tested and are therefore controlled by the same gene or by different genes in the same region of the viral dna. | 1979 | 219262 |
| effect of actinomycin d on the expression of herpes simplex virus-common surface antigen in cells transformed by herpes simplex virus type 2. | using rabbit antiserum hyperimmune to herpes simplex virus (hsv) type 1, the expression of hsv-common surface antigen(s) was studied by indirect immunofluorescence tests in cells transformed by hsv type 2 and in derived tumor cells. the following results were obtained. (i) antiserum to hsv type 1 reacted specifically with surface antigen present on the plasma membrane of both hsv type 2-infected and hsv type 2-transformed hamster cells. (ii) the expression of this antigen was enhanced in the abs ... | 1979 | 219215 |
| identification of disulfide-linked protein complexes in the nucleocapsids of herpes simplex virus type 2. | 1979 | 222065 | |
| persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). | 1979 | 222414 | |
| analysis of chromosomes, nucleic acids, and polypeptides in hamster cells transformed by herpes simplex virus type 2. | syrian hamster embryo fibroblasts were oncogenically transformed by uv-inactivated herpes simplex type 2. eighteen clones were isolated shortly after transformation occurred. two clones and their tumor derivatives were studied using several techniques. the karyotype analysis revealed different chromosome patterns in the two clones and a tendency toward hypodiploidy in the tumor derivatives. all of these cell lines were shown by molecular hybridization to contain 40% of the hsv-genome in several ... | 1979 | 222446 |
| partial characterization of herpes simplex virus type 2 (hsv-2)-specific poly(a)+ rna by hybridization to ecori-generated hsv-2 dna fragments. | 1979 | 218358 | |
| extreme sensitivity of enveloped viruses, including herpes simplex, to long-chain unsaturated monoglycerides and alcohols. | unsaturated monoglycerides and alcohols of chain lengths of 16 or 18 carbons were found to be extremely potent inactivators of two enveloped viruses, herpes simplex virus type 2 and bacteriophage phi6. the lipid-containing bacteriophage pm2 was also inactivated by some of these amphiphilic molecules. treatment of herpes simplex virus type 2 with these compounds at concentrations as low as 0.2 mum reduced virus survival to 50% in 30 min, making these agents the most potent inactivators of herpes ... | 1979 | 218499 |
| herpes simplex virus (hsv) infection of organotypic nerve cell cultures. tubule-like structures in the nuclei of cells infected with type 2 but not with type 1 virus. | in hsv-2 infected cultures, intranuclear tubule-like structures were found more commonly in fibroblasts and central nervous system (cns) neurons than in oligodendrocytes, astrocytes, satellite and schwann cells, but were found least often in peripheral neurons. | 1979 | 525261 |
| herpes simplex virus infection: problems and prospects as perceived by a peripatetic pediatrician. | the multivaried aspects of the herpes simplex viruses (hsv) types 1 and 2 and the infections they produce are discussed. points emphasized are: (1) the need for considering these (and other viruses) from an evolutionary perspective; (2) the necessity of disseminating current methods for virus identification; (3) the great progress in molecular-virological aspects and in the genetics of the virus which provide new tools for epidemiological and immunological studies and define more convincingly th ... | 1980 | 6246686 |
| comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains. | this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ... | 1980 | 6246854 |
| use of [125i]deoxycytidine to detect herpes simplex virus-specific thymidine kinase in tissues of latently infected guinea pigs. | the footpad skin and the lumbosacral dorsal root ganglia were removed from inbred guinea pigs at different times after subcutaneous infection with herpes simplex virus type 2 (hsv-2) in both hind footpads. these tissues, shown by our previous study to harbor latent hsv, were dispersed into single cells. the presence of virus-specific thymidine kinase (tk) in these cells was assayed by the uptake and phosphorylation of [125i]deoxycytidine in culture. [125i]deoxycytidine was shown to be a specific ... | 1980 | 6247502 |
| genetic retrieval of viral genome sequences from herpes simplex virus transformed cells. | oncogenic transformation of cultured cells by inactivated herpes simplex virus (hsv) types 1 and 2 has been demonstrated. expression of hsv information in these transformed cells has been shown by immunofluorescence studies, detection of hsv neutralizing antibody in sera from tumour-bearing animals and by hybridization of hsv-specific rna. molecular hybridization studies of dna from hsv-2 transformed hamster cells have detected up to 40% of the hsv genome present in several copies. complementati ... | 1980 | 6247659 |
| immunoprecipitation of polypeptides from hamster embryo cells transformed by herpes simplex virus type 2. | 1980 | 6249032 | |
| comparison of foldback sequences of herpes simplex virus types 1 and 2 dna. | the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ... | 1980 | 6243346 |
| suppression by progesterone of nonspecific in vitro lymphocyte stimulation in mice as a mechanism for the enhancement of herpes simplex virus type 2 vaginal infection. | the role of antibody, interferon, and cell-mediated immunity (cmi) were studied to determine the mechanisms for progesterone enhancement of vaginal herpes simplex virus type 2 (hsv 2) infection in mice. three groups of mice were studied: nonpregnant control, pregnant, and nonpregnant progesterone-treated mice. vaginal infection with hsv 2 did not elicit a neutralizing antibody or a systemic interferon response in any of the groups tested. splenic lymphocytes from noninfected and infected mice we ... | 1980 | 6243861 |
| characterization of herpes simplex virus strains isolated from patients with various diseases. | herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ... | 1980 | 6243918 |
| different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits. | skin infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ... | 1980 | 6244227 |
| some characteristics of an early protein (icp 22) synthesized in cells infected with herpes simplex virus. | in vero cells incubated at 40 degrees c or treated with azetidine at 37 degrees c, synthesis of a polypeptide ('c') of apparent mol. wt. 66000 was stimulated. it was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. in cells infected with herpes simplex virus type 1 (hsv-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide c, was suppressed and infected cell polypeptides icp 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and ... | 1980 | 6245173 |
| collagenase levels in a new model of experimental herpetic interstitial keratitis. | a reproducible model of severe herpetic interstitial keratitis was developed by injecting live herpes simplex virus type 2 intrastromally into the corneas of presensitized rabbits. herpes-infected corneas showed significantly more stromal infiltration and vascularization, iritis, conjunctivitis, and epithelial disease than the control corneas injected with cell supernatant without virus. levels of total collagenase detected in the culture media of the herpes-infected corneas were high and were s ... | 1980 | 6246865 |
| oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | 5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ... | 1980 | 6248643 |
| differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. | fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ... | 1980 | 6249749 |
| ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2. | the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ... | 1980 | 6249764 |
| tumorigenic transformation induced by a specific fragment of dna from herpes simplex virus type 2. | transfection of syrian hamster embryo cells with limit digests of bgl ii-, hpa i-, or bgl ii/hpa i-cleaved dna from herpes simplex virus type 2 (strain s-1) but not with salmon sperm dna resulted in the appearance of refractile, morphologically altered cells at a frequency of 10(-5)/0.005 microgram of viral dna within two to four passages. transformed lines manifested reduced serum requirement and anchorage-independent growth and were tumorigenic in newborn hamsters. they expressed icp10, a vira ... | 1980 | 6246534 |
| specific viral antigens in rat cells transformed by herpes simplex virus type 2 and in rat tumors induced by inoculation of transformed cells. | a rat cell line, re1, oncogenically transformed by a herpes simplex virus type 2 ts mutant (ts 1), has been demonstrated to contain herpes simplex virus type 2-specific thymidine kinase activity, as have two of four tumors induced in rats by inoculation of these transformed cells. a high proportion of sera from tumor-bearing rats (5 of 11) have detectable antibody against herpes simplex virus thymidine kinase, and there is a correlation between enzyme activity in a tumor and antibody in "tumor s ... | 1980 | 6245808 |
| experimental type 2 herpes simplex ophthalmitis in the newborn rat. | an animal model for the study of type 2 herpes simplex virus (hsv2) ophthalmitis is described. wistar rats were inoculated intracerebrally with hsv2 at 0, 5, 10, 15, 20, or 30 days of age. eyes and brain from all animals, whether they survived or succumbed with encephalitis, were collected for microscopic and virologic studies. up to 20% or more of the hsv2-inoculated rats had lesions in the cornea, uveal tract, and/or retina. herpetic keratiis occurred in a few animals while the eyelids were st ... | 1980 | 6246021 |
| an evaluation of herpes simplex virus antigenic markers in the study of established and developing cervical neoplasia. | previous reports from our laboratory have shown that antiserum to "pure" ag-e, a type-common hsv antigen, specifically stains atypical cervical cells in indirect immunofluorescence. these observations have been confirmed and extended. antisera were prepared against the two protein components of pure ag-e, designated icp 12 (m. w. = 140,000) and icp 14 (m. w. = 130,000), and were purified to radiochemical homogeneity by sds-acrylamide gel electrophoresis. the antisera reacted as well as antiserum ... | 1980 | 6255843 |
| double infection of balb/c mice with rauscher murine leukemia and herpes simplex virus type 2. | when balb/c mice were first infected with rauscher murine leukemia virus (mulv-r) and then superinfected with herpes simplex virus type 2 (hsv-2) the inhibition of the evolution of splenomegaly was observed. the effect did not depend on the route of hsv-2 administration. experiments in which potent anti-interferon serum was administered to double infected mice suggested that the antagonism between mulv-r and hsv-2 was not mediated by endogenous interferon, hsv-2 was found to replicate in the lps ... | 1980 | 6255890 |
| macrophage extrinsic antiviral activity during herpes simplex virus infection. | peritoneal macrophages from mice infected with herpes simplex virus type 2 (hsv-2) exhibited extrinsic antiviral resistance. when the macrophages were co-cultivated in vitro with virus-infected cells the yield of virus was reduced markedly. activity was not present 1 to 2 days p.i., peaked at 3 to 4 days, declined by 7 days and was absent at 14 days after hsv-2 infection. the extrinsic antiviral activity was limited to the adherent peritoneal macrophage population. the macrophage antiviral activ ... | 1980 | 6247426 |
| association of herpes simplex thymidine kinase gene with chromosome no. 18 in transformed human cells. | the herpes simplex virus type-2 (hsv-2)-transformed human cell line hb-2-3 was fused with thymidine kinase (tk)-deficient mouse cells [lm(tk-)], and 12 independent hybrids were isolated with the use of the hat (hypoxanthine, amethopterin, and thymidine)-ouabain selection system. discontinuous polyacrylamide gel electrophoresis studies demonstrated that the hsv-2-specific tk was the selected enzyme in the hybrids. isoenzyme analysis and karyotyping were used in the analysis of the hybrids for the ... | 1980 | 6243715 |
| analysis of viral dna sequences in hamster cells transformed by herpes simplex virus type 2. | herpes simplex virus type 2 (hsv-2) dna was treated with four restriction endonucleases (ecori, hinciii, bgl ii, and xba i) and eight fragments were purified and labeled with 32p in vitro. the kinetics of renaturation of each of the fragments was measured in the presence of dna extracted from 333-8-9, a hamster cell line transformed by uv light-inactivated hsv-2 strain 333, and from a series of cloned derivatives and their tumor lines. all of the lines examined contained a partial set of viral s ... | 1980 | 6244590 |
| resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci. | acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ... | 1980 | 6246532 |