TitleAbstractYear(sorted ascending)
recurrent herpetic whitlow due to herpes simplex virus type 2.herpes simplex virus type 2 was demonstrated to be the cause of a recurrent painful and disabling digital infection in a young man. it is possible that this was originally venereally transmitted. with increasing incidence of genital herpes infections, digital herpetic infections may become more common in the nonmedically employed community.1978686934
antibody-mediated recovery from subcutaneous herpes simplex virus type 2 infection.young adult mice infected with 3 x 10(6) plaque-forming units of herpes simplex virus type 2 (hsv-2) died within 9 to 12 days after spread of the virus from the sites of infection to the spinal cord and brain. administration of hsv-2-neutralizing antisera prepared in syngenic mice or in rabbits inhibited spread of the virus from the footpad of infected animals and prevented death. a single intraperitoneal inoculum of antiserum (virus-neutralizing titer of 1:128) was effective in protecting mice ...1978689732
preliminary studies of the mode of action of arildone, a novel antiviral agent.arildone (also known as win 38020), a novel aryl diketone, inhibited replication of herpes simplex virus type 2 in tissue culture by interfering with an event that occurs prior to 6 h postinfection. the inhibition could be partially reversed by washing. although the exact mechanism of action is unknown, neither viral deoxyribonucleic acid nor viral proteins were synthesized in the presence of arildone.1979224808
presence of herpes simplex virus type 2 dna in hamster cells oncogenically transformed by ultraviolet-inactivated virus.herpes simplex virus type 2 (hsv-2) dna has been detected by molecular hybridization in hamster fibroblast cells oncogenically transformed by ultraviolet-irradiated virus. at early passages after cloning in soft agar, about 40% of the hsv-2 genome was present in all the transformed cell lines at one to six copies per cell. in cell lines derived from tumors induced by these cells, the same percentage of the hsv-2 genome was also found with more uniform number of copies (between two and three). th ...1979224988
lymphocyte transformation for the detection of herpesvirus-induced tumor-associated antigens.cell-mediated immunity to fibroblasts transformed by herpes simplex virus type 2 was investigated with a lymphocyte assay system. the assay system was first standardized with phytohemagglutinin, a nonspecific stimulator of blastogenesis. hamster splenic and blood lymphocytes reacted to phytohemagglutinin with a dose-response curve similar to that reported for other rodent species. splenic lymphocytes from hamsters bearing isografts, induced by herpes simplex virus type 2, were transformed by cel ...1979225013
phosphorylation of arabinofuranosylthymine in non-infected and herpesvirus (tk+ and tk-)-infected cells.the phosphorylation of arabinofuranosylthymine (arathd) has been studied both in non-infected cells and in those infected with herpes simplex virus (hsv-1, lennette; hsv-1, ies and hsv-2, d-316). in these experiments, hsv strains were used which either contain (lennette, tk+ and d-316 tk+) or lack (ies, tk-) the capacity to induce pyrimidine deoxyribonucleoside kinase. it was found that extracellularly administered arathd is phosphorylated to ara ttp via aratmp and aratdp in both non-infected an ...1979225422
neoplastic transformation of cultured syrian hamster embryo cells by dna of herpes simplex virus type 2.syrian hamster embryo cells were transformed to a neoplastic phenotype after exposure to herpes simplex virus type 2 (s-1) dna at concentrations (less than or equal to 0.01 microgram per 60-mm dish) at which infectivity was no longer demonstrable. transformed cells manifested in vitro phenotypic properties characteristic of the neoplastic state, expressed herpes simplex virus-specific antigens, and induced invasive tumors in vivo. transfection and transformation of syrian hamster embryo cells wi ...1979225525
physical mapping of paar mutations of herpes simplex virus type 1 and type 2 by intertypic marker rescue.mutations (paar) in herpes simplex virus (hsv) which confer resistance to phosphonoacetic acid involve genes associated with virus-induced dna polymerase activity. two mutants of hsv (hsv-1 tsh and hsv-2 ts6) produce a thermolabile dna polymerase activity. in this study, the ts lesions present in these mutants and those present in two independent phosphonoacetic acid-resistant mutants of hsv-1 and hsv-2 (paar-1 and paar-2) have been physically mapped by restriction endonuclease analysis of recom ...1979225553
effects of cannabinoids on host resistance to listeria monocytogenes and herpes simplex virus.previous investigations from our laboratories have demonstrated that cannabinoids possess immunosuppressive properties. the present studies were designed to determine whether these agents decrease host resistance to infections with listeria monocytogenes and herpes simplex virus type 2. host resistance was measured by changes in the 50% lethal dose of the pathogen in cannabinoid-treated and control mice. the effect of cannabinoids on resistance to l. monocytogens was dose dependent. delta-9-tetr ...1979313368
immunoelectrophoretic identification and purification of herpes simplex virus antigens released from infected cells in tissue culture.proteins released from herpes simplex virus type 1 (hsv-1)-and type 2 (hsv-2)-infected hep-2 cells have been characterized by the crossed immunoelectrophoretic technique. both hsv type-common and type-specific antigens were found in the tissue culture medium 24 h after infection. antigen ag-6, an hsv-1-specific antigen, was found in high concentration in the medium as compared to other hsv antigens released from hsv-1-infected cells. the hsv-2-specific antigens, ag-4 and ag-1, were released in m ...197985610
a rapid technique for distinguishing herpes-simplex virus type 1 from type 2 by restriction-enzyme technology.a rapid simplified technique, based on restriction-endonuclease analysis of radioactively labelled d.n.a. is described; it distinguishes herpes-simplex virus type 1 strains from herpes-simplex virus type 2 unambiguously. large numbers of isolates can be screened in 4--5 days.197986095
production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2.we prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. cell lines 1d4 and 3e1, respectively, secreted immunoglobulin g1 herpes simplex virus type 1 and immunoglobulin g1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. in contrast, guinea pig antisera prepared against either herpes sim ...197991687
detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay.a micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). the test is performed by coating wells of microtiter plates with purified hsv-specified glycoproteins, vp123 and vp119, derived from hsv-1- and hsv-2-infected cells, respectively. the coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human igg reagent. because these m ...197994044
herpes simplex virus type 2 and carcinoma of urinary bladder. 197994309
iontophoretic application of adenine arabinoside monophosphate for the treatment of herpes simplex virus type 2 skin infections in hairless mice. 197994335
microfluorometric quantitation of size, deoxyribonucleic acid and viral antigenic determinants in cells productively infected with hsv-2.the interaction of herpes simplex virus type 2 (hsv-2) with the eukaryotic human cell line (hep-2) was investigated by flow microfluorometric analysis. the three parameters that were quantitated include cell size, deoxyribonucleic acid content and the expression of virus-specific antigens. productively infected cells are always smaller than uninfected ones, and they resolve into two populations with respect to viral antigenic content. consistent with viral replication, one of these two populatio ...197994525
detection of herpes simplex virus infection of female genitalia by the peroxidase-antiperoxidase method alone or in conjunction with the papanicolaou stain.the sensitive peroxidase-antiperoxidase (pxapx) method individually and in conjunction with the papanicolaou (pap) stain was used to detect herpes simplex virus (hsv) in specimens from human female genitalia. initial studies using a model system of hsv-1 or hsv-2-infected vero cells established (i) acetone as the most effective fixative, (ii) optimal dilutions of preimmunization and anti-hsv serum for differentiation of infected from noninfected cells, (iii) optimal concentration of 3,3'-diamino ...197994600
life-threatening pharyngitis caused by herpes simplex virus, type 2.a 21-year-old man who had pharyngitis needed hospitalization for observation of airway patency and for parenteral fluid therapy. infection with herpes simplex virus, type 2 (hsv-2), was diagnosed on the basis of the finding of significantly increased igm indirect hemagglutination antibody titers. the pharyngitis appeared to have been contracted by heterosexual orogenital contact (cunnilingus). the results of this case study indicate that hsv-2 infection should be considered in the differential d ...1979221994
identification of disulfide-linked protein complexes in the nucleocapsids of herpes simplex virus type 2. 1979222065
persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). 1979222414
analysis of chromosomes, nucleic acids, and polypeptides in hamster cells transformed by herpes simplex virus type 2.syrian hamster embryo fibroblasts were oncogenically transformed by uv-inactivated herpes simplex type 2. eighteen clones were isolated shortly after transformation occurred. two clones and their tumor derivatives were studied using several techniques. the karyotype analysis revealed different chromosome patterns in the two clones and a tendency toward hypodiploidy in the tumor derivatives. all of these cell lines were shown by molecular hybridization to contain 40% of the hsv-genome in several ...1979222446
experimental encephalitis in the newborn rat due to herpes simplex virus type 2. 1979222606
thin-layer immunoassay for determination of antibodies to herpes simplex virus.thin-layer immunoassay (tia) is a simple serological technique suitable for analysis of large numbers of samples. in this study, tia was evaluated for determination of antibodies to herpes simplex virus. herpes simplex virus antigen used in tia was purified from material released from virus-infected cells. the results obtained by tia were compared with those obtained by neutralization and complement fixation tests. tia was found to be as sensitive as the neutralization test for demonstration of ...1979222798
herpes virus infection as a cofactor in carcinogenesis: supernatants of herpes virus type 1- and type 2-infected cell cultures contain a cell growth-stimulating factor.some cell cultures synthesize a mitogenic factor (dnasf) following their lytic infection with either hsv-1 or hsv-2 which is shed into the medium. of six cell lines permissive to hsv infection tested, three produce dnasf (cv1, hf, mca) and a significantly higher (3h)-tdr incorporation was obtained with indicator cells growing in supernatants of virus-infected cells (supv+) than in the corresponding supernatants of sham-treated cells (supv-). sometimes the values obtained with supv+ exceed those ...1979223098
some aspects of the role of viruses in cancer.the cells of tumours induced by many oncogenic dna viruses, or cells transformed in vitro, contain virus-specific t and transplantation antigens; these have been described for sv40 virus, polyoma virus and adenoviruses. the investigation of viruses as causes of malignant disease in man has sought to establish whether tumour cells possess these virus-specific proteins; however, to date and with the limitations of present techniques, this enquiry has not demonstrated the above viruses as causal of ...1979223141
stimulation of human cellular thymidine kinase activity during abortive infection with herpes simplex virus type 2. 1979223308
association of herpes simplex virus (hsv) with cervical cancer by lymphocyte reactivity with hsv-1 and hsv-2 antigens.twenty-six women with invasive cervical cancer were examined for lymphocyte responsiveness to phytohemagglutinin (pha) herpes simplex virus (hsv). blood specimens were obtained from each patient before radiation therapy and separated into subpopulations by ficoll-hypaque centrifugation. lymphocytes were cultured in rpmi-1640 containing autologous plasma and exposed to pha, hsv-1, hsv-2, and control antigens. the results compared with those of women having negative papanicolaou smears and matched ...1979223438
sites of integration of herpes simplex virus type-2 thymidine kinase gene in human chromosomes. 1979223994
characterization of human tk- cell lines transformed to a tk+ phenotype by herpes simplex virus type 2 dna.human tk- cells carrying the hsv-2 tk gene as a result of transformation with virus dna express a tk activity of virus origin and maintain the tk+ phenotype when grown in hat medium. under non-selective conditions, however, reversion to a tk- phenotype occurs with a significant frequency characteristic of each transformed line. once reversion has occurred the tk- phenotype appears to be stable, since only very rare instances of tk- to tk+ reversion have been observed. tk- revertants were suscept ...1979215706
partial characterization of herpes simplex virus type 2 (hsv-2)-specific poly(a)+ rna by hybridization to ecori-generated hsv-2 dna fragments. 1979218358
extreme sensitivity of enveloped viruses, including herpes simplex, to long-chain unsaturated monoglycerides and alcohols.unsaturated monoglycerides and alcohols of chain lengths of 16 or 18 carbons were found to be extremely potent inactivators of two enveloped viruses, herpes simplex virus type 2 and bacteriophage phi6. the lipid-containing bacteriophage pm2 was also inactivated by some of these amphiphilic molecules. treatment of herpes simplex virus type 2 with these compounds at concentrations as low as 0.2 mum reduced virus survival to 50% in 30 min, making these agents the most potent inactivators of herpes ...1979218499
herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. 2. electron microscopic observations of myelin degeneration.organotypic cultures of mouse spinal cord with attached dorsal root ganglia, which contain both central and peripheral myelin in the one unit of tissue, were infected with hsv 1 or hsv 2 and studied using electron microscopy. intranuclear viral nucleocapsids and intracytoplasmic enveloped particles were found in the schwann cells associated with peripheral myelin and in oligodendroglia associated with central myelin. degeneration of peripheral myelin most commonly involved an asymmetrical swelli ...1979219153
effect of actinomycin d on the expression of herpes simplex virus-common surface antigen in cells transformed by herpes simplex virus type 2.using rabbit antiserum hyperimmune to herpes simplex virus (hsv) type 1, the expression of hsv-common surface antigen(s) was studied by indirect immunofluorescence tests in cells transformed by hsv type 2 and in derived tumor cells. the following results were obtained. (i) antiserum to hsv type 1 reacted specifically with surface antigen present on the plasma membrane of both hsv type 2-infected and hsv type 2-transformed hamster cells. (ii) the expression of this antigen was enhanced in the abs ...1979219215
herpes simplex virus type 2 functions expressed during stimulation of human cell dna synthesis.experiments were designed to identify herpes simplex virus type 2 (hsv-2)-specific functions expressed during stimulation of human embryo fibroblast dna synthesis. cultures were partially arrested in dna synthesis by pretreatment with 5-fluorouracil and maintenance in low-serum (0.2%) medium during virus infection. results showed that continuous [methyl-(3)h]thymidine uptake into cellular dna was ninefold greater in hsv-2-infected than in mock-infected cultures measured after 24 h of incubation ...1979219236
molecular genetics of herpes simplex virus. ii. mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells.we have mapped the location in herpes simplex virus (hsv) dna of (i) three mutations at different loci (syn loci) which alter the social behavior of infected cells from clumping of rounded cells to polykaryocytosis, (ii) a mutation which determines the accumulation of one major glycoprotein [vp8.0(c(2))], and (iii) the sequences encoding four major virus glycoproteins [vp8.0(c(2)), vp7(b(2)), vp8.5(a), and vp19e(d(2))]. the experimental design and results were as follows. (i) analysis of hsv-1 x ...1979219254
anatomy of herpes simplex virus dna. xi. apparent clustering of functions effecting rapid inhibition of host dna and protein synthesis.herpes simplex virus type 2 (hsv-2) strains inhibit the synthesis of both dna and protein of the host cell more rapidly than hsv-1 strains. several intertypic hsv-1 x hsv-2 recombinants and parental strains were examined for their ability to inhibit rapidly the synthesis of host protein and dna. the two functions cosegregated in all of eight recombinants tested and are therefore controlled by the same gene or by different genes in the same region of the viral dna.1979219262
herpes simplex type 2 encephalitis concurrent with known cerebral metastases.a 62-year-old woman developed neurologic deficits 7 months after pulmonary lobectomy for alveolar cell carcinoma of the lung. ct scan of the head demonstrated two metastases with marked peritumoral edema. administration of decadron, chemotherapy and 3,000 rad cranial radiation resulted in dramatic improvement of dysphasia and right hand paresis. almost 2 months later, rhythmic, involuntary movements of the left hand developed. there was progression to multifocal seizures, grand mal seizures, pos ...1979225922
immunotherapy and drugs in neonatal disseminated herpes simplex virus type 2 infections: a mouse model. 1979226512
differential and selective inhibition of cellular and herpes simplex virus dna synthesis by arabinofuranosyladenine.the influence of 9-beta-d-arabinofuranosyladenine (beta araado) and of its anomer 9-alpha-d-arabinofuranosyladenine (alpha araado) was studied in non-infected cells and cells infected with herpes simplex virus type 1 (hsv-1) and hsv type 2 (hsv-2). alpha araado is a strong inhibitor of proliferation of non-infected cells. multiplication of hsv-1 and hsv-2 is not affected at all by alpha araado, while their growth is strongly inhibited by beta araado. alpha araado exerts no effect on the incorpor ...1979227462
demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral dna. 1979227967
production of tubular structures in vero cells infected with herpes simplex virus type 2; effects of ultraviolet light irradiation and order to investigate the nature of tubular structures specifically found in herpes simplex virus type 2 (hsv-2)-infected cells, the multiplication of hsv-2 was studied in vero cells cultured in the presence of varying concentrations of cytosine arabinoside (ara-c) and cycloheximide (ch), inhibitors of dna synthesis and protein synthesis respectively. ara-c, at a concentration of 60 micrograms/ml, inhibited the multiplication of hsv-2 by more than 99% and also prevented the appearance of tubul ...1979228001
dnase induced after infection of kb cells by herpes simplex virus type 1 or type 2. ii. characterization of an associated endonuclease activity.purified preparations of the "exonuclease" specified by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) possess an endonuclease activity. the exonuclease and endonuclease activities copurify and cosediment in a sucrose density gradient. endonuclease activity is only observed in the presence of a divalent cation, and mg(2+) or mn(2+) is equally effective as a cofactor with an optimal concentration of 2 mm. a slight amount of endonuclease activity is observed in the presence of ca(2+), wher ...1979228069
stimulation of human cell dna synthesis by defective herpes simplex virus type 2. 1979228477
[herpes simplex virus type 2 antibodies in the husbans of women with cervical cancer]. 1979228506
virucidal activity of retinal.herpes simplex virus type 2 and simian virus 40 were rapidly inactivated by retinal at micromolar concentrations. other fat-soluble vitamins, particularly vitamin a derivatives, were also active against herpes simplex virus type 2 and several lipid-containing bacteriophages.1979228596
the influence of cell type on the production of filaments by herpes simplex virus type 2. 1979228892
regulation of persistent infection with herpes simplex virus in vitro by hydrocortisone.about 1% of raji cells showed sensitivity to herpes simplex virus type 2 (hsv-2) infection when tested by infectious center assays or immunofluorescence tests, and the percentage did not change during cell passage. the addition of hydrocortisone to raji cells persistently infected with hsv-2 caused a marked increase in virus production and in the number of hsv-producing cells. in the case of hsv-1, it was shown that the addition of hydrocortisone was required to maintain persistent infection. th ...1979229255
alterations in glycosphingolipid patterns in a line of african green monkey kidney cells infected with herpesvirus.the major glycosphingolipids (gsls) of a line of african green monkey kidney cells (bgm) were characterized as glucosylceramide, lactosylceramide, galactosyl-galactosyl-glucosylceramide, and n-acetylgalactosaminyl-galactosyl-galactosyl-glucosylceramide. neutral gsls accounted for approximately 80% of the total gsls isolated. the predominant gangliosides were n-acetylneuraminyl-galactosyl-glucosylceramide, n-acetylgalactosaminyl-n-acetylneuraminyl-galactosyl- glucosylceramide, and galactosyl-n-ac ...1979229262
storage and transport of cultures for herpes simplex virus, type 2.tryptic soy broth and brain-heart infusion broth maintained herpes simplex virus within +/- 1 serial dilution of the original titer in all of 51 samples held for one to three days at 4 c. in contrast, holding temperatures of -20 c or 22 c resulted in losses of titer of 10(2) or more. holding periods as long as five days prior to inoculation did not delay the appearance of the cytopathic effect following inoculation. unmodified tryptic soy broth or brain-heart infusion broth and holding periods a ...1979229726
effects of antimetabolites on the production of tubular structures in vero cells infected with herpes simplex virus type 2.treatment of vero cells infected with herpes simplex virus type 2 with the proper concentrations of hydroxyurea (hu) reduced the production of infectious virus and markedly increased the accumulation of tubular structures in the nuclei when the drug was added within 6 hours after infection. similar accumulation of tubular structures in the infected nuclei was also observed when infected cells were treated with phosphonoacetic acid at proper concentrations. the release of hu-treated, herpes simpl ...1979229802
isolation of the major herpes simplex virus type 1 (hsv-1)-specific glycoprotein by hydroxylapatite chromatography and its use in enzyme-linked immunosorbent assay for titration of human hsv-1-specific antibodies.a 131,000 molecular weight herpes simplex virus type 1 (hsv-1) glycoprotein designated antigen number 6 (ag-6) was previously shown to possess almost exclusively hsv-1-specific antigenic sites. fused rocket and crossed immunoelectrophoresis of fractions obtained from hydroxylapatite chromatography of crude hsv-1 antigen (triton x-100-solubilized, infected tissue culture cells) showed that a subfraction of ag-6 could be separated from the other hsv antigens. enzyme-linked immunosorbent assay with ...1979230199
reactivation of herpes simplex virus type 2 from a quiescent state by human cytomegalovirus.the ability of human cytomegalovirus to stimulate replication of herpes simplex virus type 2 (hsv-2) was examined. the system used involved hsv-2-infected human embryonic lung cells under conditions (39.5-40 degrees c) in which hsv-2 remains undetectable. reactivation of hsv-2 was maximal and persisted for the longest duration when cultures were superinfected with 0.02 plaque-forming unit of human cytomegalovirus per cell. infectious hsv-2 appeared 2 days after superinfection with human cytomega ...1979230494
growth inhibition by acycloguanosine of herpesviruses isolated from human infections.inhibition by acycloguanosine (acg) of plaque formation by harpes simplex virus types 1 and 2 (hsv-1 and hsv-2), varicella-zoster virus, and cytomegalovirus was studied. seventeen clinical isolates of hsv-1 were inhibited by acg at a mean 50% inhibitory dose (id(50)) of 0.15 +/- 0.09 mum. the mean id(50) for 10 isolates of hsv-2 was 1.62 +/- 0.76 mum, and for four isolates of varicella-zoster virus it was 3.75 +/- 1.30 mum. the id(50)'s for two cytomegalovirus isolates were 100 and 160 mum, and ...1979230783
[epidemiology of carcinoma of the cervix (author's transl)].in order to explain why modes of sexual life influence the incidence of cervical carcinoma, several factors have been claimed to be involved. there is no solid basis for the hypothesis that sperm may be mutagenic. by contrast, epidemiological studies showed that partners whose life is not sedentary bring back home to their wives an increased risk of cervical carcinoma. the infectious factor does not seem to be associated with syphilis but may well be represented by herpes genitalis virus (herpes ...1979230875
host-cell response to herpes virus infection in central and peripheral nervous tissue in an organotypic nerve cell culture system, all cells in both the central and the peripheral nervous system (cns, pns) components supported replication of herpes simplex virus types 1 and 2 (hsv 1, hsv 2). in hsv 1 infection, cellular response was particularly characterized by the formation of small syncytia (which involved neurons) and by the presence of bundles of interwoven fine filaments within the nuclei of infected cells. in hsv 2 infection, groups of parallel tubules characteristically f ...1979231090
blocking of acid-fixed nuclear binding of epstein-barr virus nuclear antigen (ebna) by different dna species.treatment of epstein-barr virus-determined nuclear antigen (ebna) with dna resulted in blocking of its ability to convert acid-fixed ebna-negative cell nuclei to an ebna-positive form. epstein-barr virus (ebv) dna, herpes simplex virus type 2 (hsv-2) dna and dna isolated from three lymphoblastoid cell lines differed in their potency to block this reaction. ebv dna was found to be about three times more effective than cellular dnas in abolishing the ability of dna-cellulose-purified ebna to conve ...1979231093
the presence of herpes simplex virus dna in hamster embryo cells transformed by a temperature sensitive mutant, ts-4.hamster embryo cells transformed with a temperature sensitive mutant of hsv-2, ts-4t, were examined for the presence of viral genomes. dna-dna reassociation kinetics showed that only a small portion (11 +/- 4%) of hsv-2 was present in the transformed cells.1979231439
comparative study of herpes group virus-induced dna polymerases.a comparative biochemical study of virus-induced dna polymerases was made among the herpes group viruses: namely, herpes simplex virus (hsv) type 1 and type 2, human cytomegalovirus (hcmv) and varicella-zoster virus (vzv). although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. all these virus-induced dna polymerases could efficiently use poly(dc) . oligo(dg)12--18 and poly(da) . oligo(dt)12--18 as template-primers. however, in phospho ...1979231586
tumorgenicity of herpesvirus hominis type 2-transformed cells (line 333-8-9) in adult hamsters.inoculation of adult hamsters with greater than 1.5 x 10(2) herpesvirus type 2 (hsv-2) transformed hamster embryo fibroblasts induced rapidly growing tumours at the site of inoculation, in more than 50 per cent of animals. the tumours were examined by light and electron microscopy, and were histologically anaplastic fibrosarcomas. lung metastases were frequently found in tumour-bearing animals; these were usually only seen microscopically, and were cytologically similar to the primary tumours. t ...1979231649
serological studies on the antigenic relationship between herpes simplex virus and varicella-zoster virus.if crossreacting antibodies between varicella-zoster virus (vzv) and herpes simplex virus (hsv) exist, one would expect more positive reactions with vzv in a group of hsv positive patients than in a group of hsv negative patients. this statement can only apply to a group of individuals where positive and negative reactions with respect to hsv and vzv are evenly distributed. such a distribution can only be found among children. therefore, the relationship between hsv-1 and vzv was the only one wh ...1979232073
phosphorylation of a ribosomal protein and of virus-specific proteins in cells infected with herpes simplex cells infected with herpes simplex virus a protein associated with the small subunit of ribosomes became phosphorylated. it was not detectably labelled with 14c-amino acids added after infection and is therefore probably a cellular protein. the phosphorylated ribosomal proteins from hsv-1- and hsv-2-infected cells were indistinguishable electrophoretically and had an apparent mol. wt. of about 48 000. phosphorylation of the 48k protein was detected 2 to 3 h after infection and reached a maxim ...1979232130
virus-specific dna sequences present in cells which carry the herpes simplex virus thymidine kinase gene.two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of hsv-2-specific dna sequences. both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the hsv-2 genome. revertant cells, which contained no detectable thymidine kinase, did not contain this dna sequence. the failure of ecor1-restricted hsv-2 dna to act as a donor of the thymidine kinase gene in transformation ex ...1979232133
mutant of herpes simplex virus type 2 with temperature-sensitive lesions affecting virion thermostability and dnase activity: identification of the lethal mutation and physical mapping of the nuc-lesion.we had previously shown that a temperature-sensitive (ts) mutant of herpes simplex virus type 2 strain hg52, ts13, induced a heat-labile dnase activity in infected cells (b. francke, h. moss, m. c. timbury, and j. hay, j. virol. 26:209-213, 1978). earlier work indicated that the mutant also possessed temperature-sensitive infectivity (i. w. halliburton and m. c. timbury, j. gen. virol. 30:207-221, 1976). in this study temperature-stable revertants of ts13 have been isolated; examination of them ...1979232166
[seroepidemiological study of herpes simplex virus type 2 in adult women of costa rica].the frequency of genital herpes simplex type 2 infections in a group of twenty adult costa rican women was studied by isolation of the virus and the measurement of neutralizing antibody activity in sera. the virus could not be isolated in any of the vaginal secretions. neutralizing antibody activity to herpes virus types 1 and 2 was found in sera from sixteen subjects. an antibody ii/i index equal to or larger than 87, indicative of infection with herpes simplex type 2 was found in fifty per cen ...1979233038
herpes simplex virus isolation from pyoderma gangrenosum lesions in a patient with chronic lymphatic leukemia.herpes simplex virus type 2 was isolated and identified from the vesicular border of pyoderma gangrenosum lesions on the genital region of a patient with chronic lymphatic leukemia. dramatic relief of pain as well as quick disappearance of the vesicular margin of the lesions and of the inflammatory halo around them occurred as a result of local treatment with a solution of zinc acetate. a careful search for a viral agent should be done in every case of pyoderma gangrenosum occurring in a patient ...1979220109
evaluation of indirect hemagglutination and its inhibition in the differentiation between antibodies to herpes simplex virus types 1 and 2 for seroepidemiologic studies: use of a ii/i index threshold of 85 and an assay of type-specific antibodies.two parameters, a ii/i index threshold value of 85 and an assay of type-specific antibodies to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), were assessed, for seroepidemiologic purposes, for their efficacy in the establishment of past infections with hsv-1 and hsv-2 as indicated by the results of tests for indirect hemagglutination and its inhibition. neither criterion alone established past infection with either hsv-1 or hsv-2. however, by combination of the two parameters, antibodie ...1979220339
replication of herpes simplex virus type 2 in normal dysplastic and neoplastic human cervical epithelia. 1979221223
[comparative study of the effectiveness of the purification and concentration of herpes simplex virus types 1 and 2].the results of comparative studies on concentration and purification of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) by ficol density gradient centrifugation are presented. a two-phase distribution of extracellular hsv was established in phycol density gradient centrifugation: in zones with density of 1.110-1.114 and 1.088-1.085 g/ml. the effectiveness of purification of hsv preparations recovered from the corresponding gradient zones was determined by electron microscopy and quantit ...1979219611
persistence of herpes simplex virus types 1 and 2 in infected individuals.during a 23-month period, 18 patients with facial or genital herpetic lesions were examined; culture specimens from each patient were obtained three times per week for virologic studies. the isolated viruses were identified, and the average duration of herpesvirus in lesions was determined. herpes simplex virus type 1 (hsv-1) was isolated from facial lesions for a mean duration of 3 1/2 days. in contrast, herpes simplex virus type 2 (hsv-2) was isolated from genital lesions for a mean duration o ...1979219784
activities of 5,6-dihydro-5-azathymidine against herpes simplex virus infections in mice.5,6-dihydro-5-azathymidine (dhadt), a nucleoside antibiotic inhibitory for herpes simplex virus (hsv) in cell cultures (h. e. renis, antimicrob. agents chemother. 13: 613-617, 1978), was evaluated in mice with experimental hsv infections. dhadt protected mice infected with hsv type 1 (hsv-1) when the virus was inoculated intravenously and the drug was given by subcutaneous or oral routes. the activity observed was dependent on the dose and schedule of treatment. doses of 100 to 400 mg/kg given t ...1979426515
necrotising balanitis due to a generalised primary infection with herpes simplex virus type 2.virological studies have proved herpes simplex virus type 2 to be the cause of the severe primary infection in a 37-year-old man with necrotising balanitis. symptoms of urethritis preceded the appearance of a severe local lesion; dissemination of infection occurred and lesions developed on the skin of the scalp, neck, trunk, buttocks, arms, legs, and feet. no evidence of involvement of the central nervous system was found, and the penile and skin lesions improved rapidly after the application of ...1979427514
[modelling of viral-chemical carcinogenesis in chronic infection in mice caused by the herpes simplex type 2 virus].the influence of chronic herpetic infection of white mice caused by herpes simplex virus type 2 (hsv-2) on the blastomogenic effect of a polycyclic hydro-carbon, 20-methylcholanthrene (20-mca), was studied. solid tumors developed in 20-mca-treated mice on the side of the carcinogen administration within 81--89 days. the simultaneous administration of 70 intracerebral ld50 of hsv-2 subcutaneously into a hind leg pad and 0.1 mg 20-mca subcutaneously into the inguinal area was accompanied by a syne ...1979462927
effect of pyran on latency after herpes simplex virus infections.the immunomodulator pyran protected mice against both herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) infections. in infections of the lip with hsv-1, prophylactic administration of pyran reduced the severity of the herpetic lesions and enhanced their resolution, but did not decrease the high incidence of development of latent hsv-1 infection of the trigeminal ganglia. in vaginal infections with hsv-2, prophylactic administration of pyran either systemically or locally reduced mortality, ...1979464586
appearance of rosette-forming macrophages in the lungs of influenza virus-infected mice.approximately one fifth of the macrophages obtained from the lungs of mice infected 2 to 5 days with influenza a/hk virus were found to rosette well with either unmodified human, chicken, or guinea pig erythrocytes, but not with erythrocytes from hamsters, sheep, or mice. rosette-forming macrophages were seldom seen in suspensions from uninfected mice (3+/-3%) or mice infected 24 h previously (3+/-3%). rosette formation was not due to virus hemadsorption, as indicated by the failure of specific ...1979500196
effect of trisodium phosphonoformate in genital infection of female guinea pigs with herpes simplex virus type 2.a genital herpesvirus type 2 infection in guinea pigs has been used to evaluate the inhibitory effect of phosphonoformate on the infection. an early topical treatment prevented the appearance of vesicles and histopathological changes and no virus could be recovered. when treatment, either topical or both systemic and topical, was delayed to 24 hours post infection no therapeutic effect was observed.1979508093
haemorrhage in neonatal hepatic necrosis due to herpes infection.a case of early neonatal severe bleeding and persistent hypoglycemia with a fatal outcome is reported. the autopsy examination revealed the features of neonatal hepatic necrosis. further study by the electron microscopy indicated the presence of herpes type particles in the nucleus and cytoplasm of the remaining liver cells. serological study of the maternal blood, post partum, revelaed positive reaction to herpes simplex virus type 2 at low titer. it is believed that intrauterine herpes infecti ...1979515805
herpes simplex virus (hsv) infection of organotypic nerve cell cultures. tubule-like structures in the nuclei of cells infected with type 2 but not with type 1 hsv-2 infected cultures, intranuclear tubule-like structures were found more commonly in fibroblasts and central nervous system (cns) neurons than in oligodendrocytes, astrocytes, satellite and schwann cells, but were found least often in peripheral neurons.1979525261
correlation of the chemical structure of 4-nitroquinolines inactivating human cytomegalovirus and established in vivo carcinogenicity tests.inactivation of the infectivity of human cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) has been observed following exposure to 4-nitroquinoline 1-oxide (nqo) or its metabolite, 4-hydroxyaminoquinoline 1-oxide (haqo). the present study of the specificity of the chemical structure of 4-nitroquinolines demonstrated that both the 4-nitro and 1-oxide groups were required for inactivation of virus infectivity. reduction of the 4-nitro group to a 4-hydroxyamino group ...19806119808
inhibition of herpes simplex virus type 2-induced biochemical transformation by interferon.the effect of interferon on the biochemical transformation of thymidine kinase-deficient cells by uv-inactivated herpes simplex virus type 2 has been studied. transformation was much less sensitive to the action of interferon than virus multiplication. however, the continuous presence of a high dose of interferon (2,000 u) inhibited transformation almost completely. although we could not differentiate between the effect of interferon on fixation and expression of the virus thymidine kinase gene, ...19806154151
biochemical transformation of mouse cells by a purified fragment of marmoset herpesvirus dna.although the size of marmoset herpesvirus (marhv) dna, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (hsv-1) dna, the restriction endonuclease sites of marhv and hsv-1 dnas were quite different. a specific bamhi restriction fragment (6.2 x 10(6) daltons) of marhv dna biochemically transformed lm(tk-) mouse fibroblasts to the thymidine kinase(tk)-positive phenotype. rabbit antisera, prepared against marhv tk, inhibited marhv-induced t ...19806154671
structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2.capsids of herpes simplex virus (hsv) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed nc-1 through nc-7 in order of descending molecular weight). antibodies prepared to hsv-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against hsv-1-infected kb cells. three of the antibodies (anti-nc-1, anti-nc-2, and anti-nc-3,4) also reacted with hsv-2-infected cells. tryptic peptide anal ...19806154808
glycoproteins with type common and type specific antigenic sites excreted from cells infected with herpes simplex virus types 1 and 2.agar immunodiffusion tests demonstrated that bhk 21 cells infected with either hsv i or hsv 2 release only a few hsv-specified antigens into the extracellular fluid (infected cell released polypeptides-icrp). neutralization blocking experiments showed that the majority of antigens/(including the band ii common antigen) involved as target sites in antibody-mediated virus neutralization are present in the icrp of both hsv i and hsv 2.sds-page identified six regions of virus-specified proteins in t ...19806156995
depletion of total hemolytic complement in sera from hamsters bearing herpes simplex type 2-induced hemolytic complement (ch50) levels were compared in sera from normal hamsters and hamsters bearing tumors derived from herpes simplex virus type 2-transformed cells ch50 in normal sera ranged from 160 to 212 while ch50 in tumor bearer sera ranged from 82 to 146. preincubation of tumor bearer sera with cell surface proteins (csp) from homologous herpes simplex virus type 2-derived tumor cells resulted in a 66% depletion of ch50 whereas preincubation with heterologous herpes simplex virus ty ...19806258785
immunity to herpes simplex virus type 2 (hsv-2). i. development of virus-specific lymphoproliferative and leukocyte migration inhibition factor responses in hsv-2-infected guinea pigs. 19806258811
expression of type-common envelope antigens by herpes simplex virus type 2-transformed hamster least ten polypeptides were detected on the surface of the herpes simplex virus type 1 (hsv-1) virion. two of these polypeptides, having molecular weights of 30,000 and 33,000, were identified in two herpes simplex virus type 2-transformed cell lines (333-8-9 and 333-2-29) by immunoprecipitation and sds-page. the same two virion polypeptides previously were shown to be present in an hsv-1-transformed cell line (14-012-8-1, t-10). these observations suggest that genetic information coding for ...19806259083
cloning of herpes simplex virus 2 dna fragments in a plasmid vector.dna isolated from virions of herpes simplex type 2 (hsv-2) strain 333 was digested with various restriction enzymes and joined to the ek2 plasmid vector pbr322. the viral dna sequences present in the hybrids were analyzed by restriction enzyme mapping and hybridization to fragments of hsv-2 dna. the collection of recombinant molecules represents approx. 75% of the hsv-2-genome. in most cases, the structure of the recombinants seemed identical to the organization of authentic fragments of hsv-2 d ...19806260572
interaction of herpesvirus with spleen cell subpopulations comparison of a neurotropic and a lymphotropic virus.we studied the interaction of a neurotropic herpesvirus, herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2), and a lymphotropic herpesvirus, guinea pig herpes-like virus (hlv), with guinea pig spleen cells. both hsv-1 and hsv-2 and hlv can attach to and penetrate into b- or t-enriched cells. less than 1.4% of the total b- or t-enriched cell populations were susceptible to infection by hlv and to some degree to hsv-1 or hsv-2 as determined by infectious center assays. after specific antiserum ...19806262239
effect of corynebacterium granulosum immunopotentiation on the pathogenesis of herpes simplex virus type 2 in balb/c mice.treatment of 4- to 6-week-old, 18- to 22-g male balb/c mice with 0.6 mg of corynebacterium granulosum resulted in a significant decrease in mortality due to challenge with herpes simplex virus type 2 (hsv-2). optimal protection occurred when c. granulosum was injected 1 week before hsv-2 infection. c. granulosum-induced resistance to hsv-2 lasted up to 4.5 weeks. studies involving immune spleen cell transfer and treatment with antilymphocyte serum demonstrated the importance of cell-mediated im ...19806262241
anti-herpes simplex virus and anti-human cell growth activity of e-5-propenyl-2'-deoxyuridine and the concept of selective protection in antivirus chemotherapy.e-5-propenyl-2'-deoxyuridine (e-5-propenyl-durd) inhibited the growth of herpes simplex virus (hsv) types 1 (hsv-1) and 2 in culture. the concentration of drug required to give a 2-log reduction in virus titer was 5 microm for hsv-1 and 23 microm for hsv-2. the anti-hsv-1 activity of this agent was more potent than 5-propyl-durd, equivalent to e-5(3,3,3-trifluoropropenyl)-durd, and less potent than e-5-bromovinyl-durd. the hsv-1 mutant (b2006) lacking the ability to induce virus-specific thymidi ...19806263181
localization of the thymidine kinase gene of herpes simplex virus type 2 (333).the thymidine kinase (tk) gene of herpes simplex virus type 2 (hsv-2) has been identified on purified restriction endonuclease fragments of hsv-2 dna. these fragments were localized on the physical map of hsv-2 dna. a detailed map of the hsv-2 tk gene region has been constructed which locates the tk gene between 0.285 and 0.310 map units [3.8 kilobase pairs (kb)], whereby tk gene sequences are at least present between 0.299 and 0.303 map units (0.6 kb). mouse tk- cells that were biochemically tr ...19806263824
influence of cells and virus multiplicity on the inhibition of herpesviruses with acycloguanosine.the inhibition of herpes simplex virus type 1 (hsv-1) plaque formation by acycloguanosine (acg) was assayed in human fetal lung fibroblasts (hl), cell lines from human cervical carcinoma, rabbit cornea, and human rhabdomyosarcoma, and three green monkey kidney cell lines. the acg concentration giving 50% plaque reduction (pr50) of hsv-1 was lowest in hl. in two green monkey kidney cell lines, hsv-1 plaque formation was relatively insensitive to acg, with pr50 of 25 and 60 mum, respectively. in t ...19806265398
comparison of foldback sequences of herpes simplex virus types 1 and 2 dna.the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ...19806243346
association of herpes simplex thymidine kinase gene with chromosome no. 18 in transformed human cells.the herpes simplex virus type-2 (hsv-2)-transformed human cell line hb-2-3 was fused with thymidine kinase (tk)-deficient mouse cells [lm(tk-)], and 12 independent hybrids were isolated with the use of the hat (hypoxanthine, amethopterin, and thymidine)-ouabain selection system. discontinuous polyacrylamide gel electrophoresis studies demonstrated that the hsv-2-specific tk was the selected enzyme in the hybrids. isoenzyme analysis and karyotyping were used in the analysis of the hybrids for the ...19806243715
suppression by progesterone of nonspecific in vitro lymphocyte stimulation in mice as a mechanism for the enhancement of herpes simplex virus type 2 vaginal infection.the role of antibody, interferon, and cell-mediated immunity (cmi) were studied to determine the mechanisms for progesterone enhancement of vaginal herpes simplex virus type 2 (hsv 2) infection in mice. three groups of mice were studied: nonpregnant control, pregnant, and nonpregnant progesterone-treated mice. vaginal infection with hsv 2 did not elicit a neutralizing antibody or a systemic interferon response in any of the groups tested. splenic lymphocytes from noninfected and infected mice we ...19806243861
treatment of genital herpes simplex virus in male patients.twenty-three male patients with herpes simplex virus type 2 blisters on the prepuce, glans penis, and penile shaft were divided into 2 groups. ten patients served as controls, and thirteen patients were treated with ultrasound (1 w/cm2 for 60 sec) and herpigon on 3 consecutive days. results showed a negative virus culture after 3 days of treatment and significant reduction of the recurrent infection in the treated group while the control group experienced recurrent infection within 62--80 days. ...19806243914
characterization of herpes simplex virus strains isolated from patients with various diseases.herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ...19806243918
different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ...19806244227
distribution of sequences homologous to the dna of herpes simplex virus, types 1 and 2, in the genome of pseudorabies virus.the distribution of related sequences between the genomes of pseudorabies virus (prv) and herpes simplex virus, types 1 (hsv-1) and 2 (hsv-2), was determined. approximately 7% of the sequences in prv are shared by hsv-1 and hsv-2 dnas. by means of the southern blot technique, it was found that the homologous sequences are not sequestered in one region but are distributed throughout the prv genome. hsv-1 and hsv-2 have the greatest homology with the long unique region of prv dna and the least wit ...19806244240
reduction of cell surface fibronectin (lets protein) correlates with tumorigenicity of hamster fibroblasts transformed by herpes simplex virus type2.a series of herpes simplex virus type 2 (hsv-2)-transformed hamster cells has been examined using the technique of lactoperoxidase-catalyzed cell surface protein iodination. the amount of cell surface fibronectin (lets protein) was reduced after hsv-2 transformation, and this reduction correlated with increased tumor-forming ability.19806244242
a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein.a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ...19806244352
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