Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human herpesviruses types 6 and 7 and febrile seizures. | the frequency was studied with which human herpesviruses types 6 and 7 (hhv-6 and hhv-7) occur in the cerebrospinal fluid (csf) of patients with febrile seizures and matched control patients. csf samples were prospectively collected from a case series of patients with febrile seizures and from age-, sex-, and race-matched control patients without febrile seizures, all of whom were evaluated in the emergency department of an urban, tertiary care, pediatric medical center. using polymerase chain r ... | 1999 | 10580881 |
| hydroxyurea potentiates the antiherpesvirus activities of purine and pyrimidine nucleoside and nucleoside phosphonate analogs. | hydroxyurea has been shown to potentiate the anti-human immunodeficiency virus activities of 2',3'-dideoxynucleoside analogs such as didanosine. we have now evaluated in vitro the effect of hydroxyurea on the antiherpesvirus activities of several nucleoside analogs (acyclovir [acv], ganciclovir [gcv], penciclovir [pcv], lobucavir [lbv], (r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine [h2g], and brivudin and nucleoside phosphonate analogs (cidofovir [cdv] and adefovir [adv]). when evaluated in cy ... | 1999 | 10582877 |
| nitric oxide and macrophage antiviral extrinsic activity. | in this study we evaluated the relationship between nitric oxide (no) and macrophage antiviral extrinsic activity. macrophages activated by intraperitoneal injection of herpes simplex virus-2 (hsv-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. the extrinsic antiviral activity was observed in cultures of vero cells infected with hsv-1 and hsv-2. the no inhibitor n-monomethyl-l-arginine acetate (l-nma) impaired the antiv ... | 1999 | 10583595 |
| genital herpes and diabetic ketoacidosis: a patient report. | 1999 | 10587785 | |
| treatment of primary herpes simplex virus infection in guinea pigs by imiquimod. | imiquimod (also known as r-837 and s-26308) is an imidazoquinoline immune response modifier and is available in the us and several other countries for the treatment of external genital warts. imiquimod has no direct antiviral activity but demonstrates efficacy in several animal models of virus infection. the drug is recognized by antigen presenting cells including monocytes, macrophages, b-cells and dendritic cells and induces these cells to produce cytokines including interferon-alpha (ifn-alph ... | 1999 | 10588331 |
| isolation and characterization of an anti-hsv polysaccharide from prunella vulgaris. | a water soluble substance was isolated from a chinese herb, prunella vulgaris, by hot water extraction, ethanol precipitation and gel permeation column chromatography. chemical tests showed that the substance was an anionic polysaccharide. using a plaque reduction assay, the polysaccharide at 100 microg/ml was active against the herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), but was inactive against cytomegalovirus, the human influenza virus types a and b, the poliovirus type 1 or the ves ... | 1999 | 10588332 |
| detection of herpes simplex virus (hsv) genome using polymerase chain reaction (pcr) in clinical samples comparison of pcr with standard laboratory methods for the detection of hsv. | diagnosis of herpes simplex virus (hsv) infections is achieved by detecting the antigen and isolating the virus from the specimen, which requires 7-28 days. with the recently introduced molecular biological technique of polymerase chain reaction (pcr), the diagnosis of hsv infections has been made more rapid and specific. | 1999 | 10588457 |
| foscarnet treatment of genital infection due to acyclovir-resistant herpes simplex virus type 2 in a pregnant patient with aids: case report. | 1999 | 10589917 | |
| immune responses and protection against vaginal infection after nasal or vaginal immunization with attenuated herpes simplex virus type-2. | we compared nasal and vaginal immunizations using attenuated herpes simplex virus type-2 (hsv-2) for protection against vaginal infection with wild-type hsv-2. mice were immunized once intranasally, intravaginally after progestin (dp) treatment, or intravaginally with scarification after oestradiol treatment. compared with vaginal immunizations, nasal immunization did not increase immunoglobulin a (iga) plasma cell numbers in the vagina or elicit a higher antiviral iga titre in vaginal secretion ... | 1999 | 10594699 |
| bovine herpesvirus type 2 is closely related to the primate alphaherpesviruses. | bovine herpesvirus type 2 (bohv-2), also known as bovine mammillitis virus, is classified in the family herpesviridae, subfamily alphaherpesvirinae, and genus simplexvirus along with herpes simplex viruses type 1 and 2 (hsv-1 and hsv-2) and other primate simplexviruses on the basis of similarities in 4 genes within the 15 kb u(l) 23-29 cluster. this could be explained either by a global similarity or a recombination event that brought primate herpesviral sequences into a bovine virus. our sequen ... | 1999 | 10595411 |
| a comparison of virus isolation, indirect immunofluorescence and nested multiplex polymerase chain reaction for the diagnosis of primary and recurrent herpes simplex type 1 and type 2 infections. | 134 swabs in viral transport medium were received from 126 patients with suspected clinical hsv-1 and hsv-2 infections. they were tested by (i) nested multiplex polymerase chain reaction nmpcr (strongly positive specimens had visible bands on both rounds of pcr) without prior extraction, (ii) culture in primary rhesus monkey kidney, e6-vero, rd and hep-2 cells and (iii) antigen detection by immunofluorescence (if). antigen detection employed four novel pools (a-d) of monoclonal antibodies (mab): ... | 1999 | 10598085 |
| detection of varicella-zoster virus and herpes simplex virus by the polymerase chain reaction with degenerate primers. | varicella-zoster virus (vzv) and herpes simplex virus (hsv) are human pathogens of significance involved in multiple diseases with either typical or atypical clinical features. in neonates and immunocompromised patients these alphaherpesviruses may cause life-threatening diseases such as encephalitis. detection of vzv by virus culture is difficult. polymerase chain reaction (pcr) is quicker and more sensitive and applicable in most clinical microbiological laboratories. using degenerate primers, ... | 1999 | 10598093 |
| is placental tissue protein 17b/tip47 a new factor in cervical cancer genesis? | we identified novel members of the placental tissue protein 17 (pp17) protein family which consists of different-size variants of pp17; cdnas of pp17a and pp17b variants have also been cloned and sequence analyzed. by western-blot analysis in cervical carcinoma tissues we found overexpression of pp17b. compared to healthy controls a mean five-fold increase in the amount of pp17b was also detected in the sera of untreated cervical carcinoma patients, which declined after radical operations. in ou ... | 1999 | 10697545 |
| a study on the potential role of trichomonas vaginalis in transmission of herpes simplex virus type ii. | some strains of trichomonas vaginalis have been reported to contain virus-like particles (vlp). whether they were natural inhabitants or acquired from the host, the emerging question will be about the potential role of this protozoan in viral transmission. the present model have been established for studying the ability of t. vaginalis to acquire herpes simplex virus type ii (hsv). green monkey kidney cells (vero cells) were infected with hcv and t. vaginalis was inoculated 3 days later. the pro ... | 1999 | 10605506 |
| evaluation of anti-hsv-2 activities of barleria lupulina and clinacanthus nutans. | barleria lupulina lindl. and clinacanthus nutans (burm. f.) lindau, both belonging to the family acantaceae, are well-known medicinal plants used in thai folklore medicine. virucidal effects of organic extracts of these two plants against herpes simplex virus type 2 strain g, hsv-2 (g), the standard hsv-2 strain were noted. the extracts were assessed for intracellular activities against hsv-2 (g) and five clinical hsv-2 isolates. b. lupulina extract exhibited activity against all five isolates b ... | 1999 | 10619382 |
| new tools and old for management of stds. | molecular diagnostics, such as polymerase chain reaction (pcr), are set to revolutionize the management of sexually transmitted diseases (stds); this is what delegates heard at the lesedi afrika '99 congress. in industrialized nations, the field of stds has benefited from the new technology of nucleic-acid amplification, according to max chernesky. assays that use such technology could be up to five orders of magnitude more sensitive than immunoassays. a computer model developed by chernesky ... | 1999 | 10622309 |
| antiviral activities of various water and methanol soluble substances isolated from ganoderma lucidum. | in order to find antiviral substances from basidiomycetes, two water soluble substances, glhw and gllw, and eight methanol soluble substances, glme-1-8, were prepared from carpophores of ganoderma lucidum. these substances were examined for their activities against five strains of pathogenic viruses such as herpes simplex virus types 1 (hsv-1) and 2 (hsv-2), influenza a virus (flu a) and vesicular stomatitis virus (vsv) indiana and new jersey strains in vitro. antiviral activities were evaluated ... | 1999 | 10624872 |
| antiherpetic activities of various protein bound polysaccharides isolated from ganoderma lucidum. | to investigate antiherpetic substances from ganoderma lucidum, various protein bound polysaccharides, glhw, glhw-01, glhw-02, glhw-03, were isolated by activity-guided isolation from water soluble substances of the carpophores. these substances were examined for their antiviral activities against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) by plaque reduction assay in vitro. among them, the acidic protein bound polysaccharide, glhw-02 of a brownish substance, exhibited the most potent ... | 1999 | 10624876 |
| [herpes simplex virus types 1 and 2 (hsv-1 and hsv-2)]. | herpes simplex virus (hsv) infections are prevalent worldwide. hsv are characterized by their ability to establish and maintain latent infections that can be reactivated. various clinical presentations of hsv infections are described. mostly asymptomatic, these infections may become life-threatening when occurring in neonates or when infecting the central nervous system. accurate diagnosis of hsv infections is important and pcr is the most sensitive technique for detecting hsv. type-specific ser ... | 1999 | 10731803 |
| rapid detection of glycoprotein g gene for the diagnosis and typing of herpes simplex virus infection in genital herpes. | to develop a new, rapid, and convenient technique for the diagnosis and typing of herpes simplex virus (hsv) in genital herpes (gh). | 1999 | 10754943 |
| british co-operative clinical group national survey on diagnostic issues surrounding genital herpes. mssvd special interest group on genital herpes and the british co-operative clinical group. | to investigate the current use of diagnostic methods for genital herpes simplex virus (hsv) infection, to determine how information from these tests influences clinical practice, and to identify areas for future guideline development within genitourinary medicine (gum) clinics in the united kingdom. | 1999 | 10754945 |
| detection of herpes simplex virus dna in maternal breast milk by in situ hybridization with tyramide signal amplification. | in this study we investigated the prevalence of herpes simplex virus 1 (hsv-1) and 2 (hsv-2) in maternal breast milk using the technique of in situ hybridization combined with the sensitive detection system of tyramide signal amplification. breast milk samples were collected from both breasts of 34 puerperals 4-5 days after delivery. hsv dna was detected in 16 out of 34 examined breast milk specimens. hsv dna was found in the milk of both breasts in 10 cases (62.5%), in that of left breast in 4 ... | 1999 | 10757038 |
| synthesis of 4'-substituted nucleosides and their biological evaluation. | 4'-c-ethynyl-beta-d-arabino- and 4'-c-ethynyl-beta-d-2'-deoxy-ribo-pentofuranosyl pyrimidines were synthesized. most of these 4'-ethynyl nucleosides showed interesting biological activities. | 1999 | 10780412 |
| [current therapies of genital herpes simplex in women]. | 1999 | 10955118 | |
| an overview of sexually transmitted diseases. | brief summaries of the rate of infection and symptoms are provided for the following major sexually transmitted diseases (stds): herpes simplex virus type 2, human papilloma viruses, trichomoniasis, chlamydia, gonorrhea, hepatitis b, and syphilis. because stds increase susceptibility to hiv and other serious complications, hiv educators are advised to counsel patients on the risks of std exposure and infection. the rates by which some stds increase the risk of infection with hiv are also provide ... | 1999 | 11366673 |
| data on hiv infection in women expands. | research on the relationship between sexually transmitted diseases (stds) and hiv was presented at the 39th interscience conference on antimicrobial agents and chemotherapy (icaac). researchers have found significant associatioons between stds and hiv, including a common mode of transmission. data from several african studies on hiv are described. topics include the impact of std treatment, herpes simplex virus 2 (hsv-2), hiv in the female genital track, and cervicovaginal secretion. | 1999 | 11367336 |
| cellulose acetate phthalate (cap): an 'inactive' pharmaceutical excipient with antiviral activity in the mouse model of genital herpesvirus infection. | the spread of sexually transmitted infections caused by herpes simplex virus type 2 (hsv-2) has continued unabated. at least 20% of the united states population has been infected with hsv-2 and there is a high probability of further virus transmission by asymptomatic carriers. given the absence of effective vaccines, this indicates the need to develop prophylactic measures such as topical microbicides that have antiviral activity. recent studies indicate that cellulose acetate phthalate (cap), a ... | 1999 | 10628808 |
| bisamides from aglaia edulis. | the leaves of aglaia edulis afforded a new bisamide, aglaiduline, and two new sulfur-containing bisamides, aglaithioduline and aglaidithioduline. their structures were established from spectroscopic studies. the sulfur-containing amides exhibited slight antiviral activity against herpes simplex virus types 1 and 2. | 1999 | 10643670 |
| monoclonal antibodies suitable for type-specific identification of herpes simplex viruses by a rapid culture assay. | eight monoclonal antibodies (mabs) to herpes simplex viruses 1 and/or 2 (hsv-1, hsv-2) were tested for their reactivity with 190 previously typed hsv-positive clinical specimens in order to determine their suitability for use as type-specific reagents. using a rapid culture assay we found that two mabs (t51 and t96) identified hsv-1 in all the 94 specimens, previously found hsv-1-positive, but did not react with the 96 specimens, previously found hsv-2-positive. in contrast, one mab (303) confir ... | 1999 | 10825932 |
| a homogenous 384-well high throughput screen for novel tumor necrosis factor receptor: ligand interactions using time resolved energy transfer. | the herpes virus entry mediator (hvem) receptor and its ligand, hvem-l, are involved in both herpes simplex virus type-1 (hsv-1) herpes simplex virus type-2 (hsv-2) infection, and in t-cell activation such that antagonists of this interaction are expected to have utility in viral and inflammatory diseases. in this report we describe the configuration of a homogeneous 384-well assay based on time-resolved energy transfer from a europium chelate on the hvem receptor to an allophycocyanin (apc) acc ... | 1999 | 10838440 |
| [monoclonal antibodies in the diagnosis of infections caused by the herpes simplex virus]. | a series of hybridomas producing monoclonal antibodies (mcab), specifically interacting with herpes simplex virus (hsv) proteins, types 1 and 2, has been obtained. mcab 7c4 and 4f6 have been shown to be highly active in the solid-phase enzyme immunoassay (eia) and to produce no reaction with hsv antigen in the indirect immunofluorescent assay (iifa). mcab 2b6, 3e5, 4a, 2c effectively detect mcab in iifa, but not eia, while mcab 3d 10 exhibit activity in both biochemical assays. moreover, as esta ... | 1999 | 10852033 |
| clinical management of selected oral fungal and viral infections during hiv-disease. | the purpose of this review is to describe current possibilities of management of selected fungal and viral oral opportunistic infections including oral candidiasis, herpes simplex type 1 and 2-related lesions (hsv1,2), oral hairy leukoplakia (ohl) and oral lesions associated with human papilloma viruses (hpv). less common diseases such as cytomegalovirus infection or human herpes virus type 8 associated with kaposi's sarcoma and others are not considered. in a number of instances lifelong therap ... | 1999 | 10858761 |
| role of serology in the diagnosis of herpes simplex encephalitis. | twenty one cerebrospinal fluid (csf)/brain tissue (16 csf and 5 brain tissue) from patients clinically suspected of herpes simplex encephalitis (hse) were collected during one year period and was subjected for the detection of hsv type i and type ii antigen by direct immunofluorescence (dif). paired serum and csf samples obtained from 12 patients were tested for herpes simplex virus antibodies by indirect immunofluorescence test. of the 21 cases, two were positive for hsv-1 antigen in csf and on ... | 1999 | 10862294 |
| [the detection of the herpes simplex virus 1 and 2 antigens in clinical specimens by using monoclonal antibodies]. | the possibility of using monoclonal antibodies (mcab), obtained earlier, for the detection of herpes simplex virus (hsv) in clinical specimens taken from sick and infected persons was studied. the examination of 90 persons revealed that the mixture of mcab 4a and 2c could effectively detect the presence of hsv antigen in the indirect immunofluorescence assay (ifa) directly in cells contained in cytological preparations (smears, scrapes, impressions) obtained from different organs of patients. th ... | 1999 | 10876858 |
| [a killed commercial vaccine against herpes simplex viruses 1 and 2 as an immunocorrective agent in chronic herpetic infection]. | 1999 | 10876868 | |
| [studies on the antiviral activity of silver sulfathiazole]. | silver sulfathiazole shows strong antibacterial activity and good tolerance after topical application. the aim of the study was to determine the antiviral activity of silver sulfathiazole in tissue culture after incubation of drug and virus. the antiviral activity was measured after various periods of exposure and at different drug concentrations. the results obtained indicate the activity of silver sulfathiazole against herpesvirus type 1 and type 2. this drug suppresses or completely inactivat ... | 1999 | 10865442 |
| [study on the relationship between the history of abnormal pregnancy and torch infection in pregnant woman]. | to investigate the relationship between the history of abnormal pregnancy and the toxoplasma(tox), other(oth), rubella virus(ruv), cytomegalo virus(cmv), herpes simplex virus-ii (hsv-ii) (torch) infections in pregnant woman. | 1999 | 11360633 |
| herpes drug suppresses hsv in people with hiv. | famvir (famciclovir), manufactured by smithkline beecham pharmaceuticals, has been shown to suppress herpes simplex virus (hsv) infections in people with hiv. over a period of 4 months, 97 percent of patients treated with the drug did not experience any recurrences. the drug cleared the initial lesions, and most patients remained free of recurrences during the trial. famvir was well tolerated, with diarrhea being the most commonly reported side effect. these findings could have a major impact on ... | 1999 | 11366212 |
| ultrastructural modifications at uterine cervix level induced by human papillomaviruses infections. | the paper presents the ultrastructural modifications occurring in uterine cervix epithelium in 10 patients with clinical susceptibility of hpv infections. the presence of viral hpv particles was identified in 5 cases and in 1 case the concomitant presence of both hpv and hsv2 particles was detected. the maximum amount of hpv virions is found in the spinous and superficial layer, particularly in the nucleus, but also in cytoplasm. in all the studied cases, cellular alterations were present in the ... | 1999 | 11845447 |
| antiviral properties of fucoidan fractions from leathesia difformis. | three fractions of fucoidans isolated from the brown seaweed leathesia difformis (ee, ec and ea) were found to be selective antiviral agents against herpes simplex virus (hsv) types 1 and 2 and human cytomegalovirus. fraction ea was the most active, with ic50 values in the range 0.5-1.9 microg/ml without affecting cell viability at concentrations up to 400 microg/ml. the antiherpetic activity of ea was assessed by three different methods, plaque reduction, inhibition of virus yield and preventio ... | 1999 | 11962540 |
| sexual behaviour, stds and drug use in a crack house population. | we aimed to investigate the prevalence of ulcerative sexually transmissible diseases (stds) and hepatitis in crack users. we interviewed 435 crack users on site in crack houses in houston, texas and took blood for laboratory analysis. there was evidence of syphilis infection in 13%, herpes simplex virus-2 (hsv-2) in 61%, hiv in 12%, hepatitis b in 52%, and hepatitis c in 41% of cases. on dsm-iii-r criteria, 12% were crack abusers and 84% crack dependent: over half reported previous treatment. fo ... | 1999 | 12035774 |
| [detection of human papillomavirus and herpesvirus genotypes in biopsy specimens from cervical carcinoma by pcr-endonuclease cleavage]. | this study was to detect the human papillomavirus (hpv) and herpes simplex virus (hsv) genotypes in biopsy specimens of cervical carcinoma and genital verruca by pcr-endonuclease cleavage. | 1999 | 12569753 |
| sero-prevalence of herpes simplex virus type 2 (hsv-2) in rakai district, uganda. | herpes simplex virus type 2 (hsv-2) is sexually transmitted and causes one of the genital ulcerative diseases associated with the transmission of human immunodeficiency virus type i (hiv-1). | 2000 | 12862067 |
| stds: flushing out the "hidden epidemic". | the rapid explosion of hiv infection ranks as the most significant event in the arena of sexually transmitted diseases (stds). in making efforts against hiv/aids, public health officials are now preparing to confront what is called "the hidden epidemic" of stds. important progress has been made in research in diagnosing and treating stds including urine-based screening for gonorrhea, single-dose treatments for curable stds, as well as improved chlamydia screening and treatment. while herpes si ... | 2000 | 12295506 |
| inactivation of hsv-1 and hsv-2 and prevention of cell-to-cell virus spread by santolina insularis essential oil. | the essential oil obtained in toto from santolina insularis was investigated for its antiviral activity on herpes simplex type 1 (hsv-1) and type 2 (hsv-2) in vitro. the ic(50) values, determined by plaque reduction assays, were 0.88 and 0.7 microg/ml for hsv-1 and hsv-2, respectively, while the cc(50) determined by the mtt test on vero cells was 112 microg/ml, indicating a cc(50)/ic(50) ratio of 127 for hsv-1 and 160 for hsv-2. results obtained by plaque reduction assays also indicated that the ... | 2000 | 11164504 |
| [corporal distribution of infections caused by herpes simplex virus types 1 and 2]. | 2000 | 11198010 | |
| guanosine analogues as anti-herpesvirus agents. | several guanosine analogues, i.e. acyclovir (and its oral prodrug valaciclovir), penciclovir (in its oral prodrug form, famciclovir) and ganciclovir, are widely used for the treatment of herpesvirus (i.e. hsv-1, hsv-2, vzv and hcmv) infections. in recent years, several new guanosine analogues have been developed, including the 3-membered (cyclopropyl) sugar derivative a-5021 and the 6-membered d- and l-cyclohexenyl derivatives. prominent features shared by all guanosine analogues are the followi ... | 2000 | 11200257 |
| herpes simplex virus type-2 antibodies in pregnant women: the impact of the stage of pregnancy. | in this study the impact of pregnancy duration on the measured level of hsv-2 antibodies was assessed. the study population comprised 35,940 pregnant women in norway, in 1992-4, followed during pregnancy. a random sample of 960 women was selected. a mean of 2.6 serum samples from each woman were analysed for hsv-2 specific igg antibodies at different times in pregnancy. crude and adjusted odds ratios were estimated in logistic regression models taking all observations per women into account. twe ... | 2000 | 11218218 |
| [serological diagnostic trial of the causative virus of bell's palsy by anti-herpes virus antibodies in the paired sera]. | substantial evidence obtained through polymerase chain reaction techniques strongly supports that reactivation of latently infected herpes simplex virus (hsv) in geniculate ganglia is the main cause of bell's palsy. however, serum antibody titers to hsv rarely increase in patients with this disease. this discrepancy may result from the difficulty in detecting a small increase in antibody titers by conventional serological analysis. to detect such a small increase in antibody titer, we defined th ... | 2000 | 11218698 |
| frequency of herpes simplex virus in syria based on type-specific serological assay. | this study aimed to determine the seroprevalence of herpes simplex virus types 1 and 2 antibodies in syrian populations. serum samples collected between 1995-1998 from a total of 1293 healthy persons, neonates, pregnant women, labial herpes patients, kidney transplantation patients, atherosclerosis patients, cervical cancer patients, patients with suspected herpetic keratitis, viral encephalitis patients, patients with sexually transmitted diseases, aids patients, female prostitutes and bar girl ... | 2000 | 11533818 |
| superiority of intramuscular route and full length glycoprotein d for dna vaccination against herpes simplex 2. enhancement of protection by the co-delivery of the gm-csf gene. | immunization with naked dna has been analyzed in two critical variables: the site of injection and the cellular compartment to which the coded protein is directed. the gene for the full length of the glycoprotein d (gd) of hsv-2 under the control of the citomegalovirus (cmv) promoter was injected via the intradermal (i.d.) or the intramuscular (i.m.) routes in mice. immunization in the quadricep muscle was superior to the intradermal immunization in the footpads. a stronger activation of ifn-gam ... | 2000 | 10869769 |
| molecular evidences for a role of hsv-1 in multiple sclerosis clinical acute attack. | to verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (rrms) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (pbmcs) collected from 22 ms patients during an ms relapse and in a stable phase and from 18 healthy controls (hc). dna belonging to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hu ... | 2000 | 10871797 |
| serological prevalence of herpes simplex virus type 2 amongst gum clinic attenders in a district general hospital setting. | our objective was to determine the seroprevalence of herpes simplex virus (hsv) type 2 infection amongst genitourinary medicine (gum) clinic attenders at a district general hospital using a commercially available enzyme immunoassay (eia). in a prospective study, heterosexual patients attending the department of gum at trafford general hospital attending with a new clinical problem and having a blood sample taken for routine syphilis serology had the same sample tested for hsv type 2 antibodies. ... | 2000 | 10872911 |
| reductions in herpes simplex virus type 2 antibody titers after cognitive behavioral stress management and relationships with neuroendocrine function, relaxation skills, and social support in hiv-positive men. | coinfection with herpes simplex virus type 2 (hsv-2) is common in individuals infected with human immunodeficiency virus (hiv) and may have health implications. this study examined the effect of a 10-week cognitive behavioral stress management (cbsm) intervention on immunoglobulin g (igg) antibody titers to hsv-2 in a group of mildly symptomatic hiv-infected gay men and the degree to which these effects were mediated by psychosocial and endocrine changes during the 10-week period. | 2000 | 11139003 |
| an evaluation of near patient tests for detecting herpes simplex virus type-2 antibody. | to examine the "in use" test characteristics of the pockit "near patient" hsv-2 rapid test for the detection of hsv-2 igg antibodies for use in the clinic. this test relies on a visual interpretation of the result. | 2000 | 11141856 |
| current recommendations for the treatment of genital herpes. | the incidence of genital herpes continues to increase in epidemic-like fashion. aciclovir (acyclovir) has been the original gold standard of therapy. the recent addition of famciclovir and valaciclovir as antiherpes drugs has improved convenience as well as the efficacy of treatment. although aciclovir remains a widely prescribed and reliable drug, its administration schedule falls short of the ease of usage that the newer nucleoside analogues offer, for both episodic and suppressive therapy. su ... | 2000 | 11152015 |
| herpes simplex virus-type 2 seropositivity in a danish adult population denying previous episodes of genital herpes. | 2000 | 10877149 | |
| detection of herpes simplex virus dna by real-time pcr. | molecular detection of herpes simplex virus (hsv) dna is recognized as the reference standard assay method for the sensitive and specific diagnosis of central nervous system infections caused by hsv. in this study, a molecular assay based on real-time pcr on the lightcycler (lc) instrument was evaluated and compared with a home-brew molecular assay. the detection limit of the lc assay was determined with 10-fold dilutions of plasmid ps4 with the sali restriction fragment of the dna polymerase ge ... | 2000 | 10878056 |
| long term persistence of herpes simplex virus-specific cd8+ ctl in persons with frequently recurring genital herpes. | herpes simplex virus (hsv) establishes a lifelong infection in humans. reactivation of latent virus occurs intermittently so that the immune system is frequently exposed to viral ag, providing an opportunity to evaluate memory t cells to a persistent human pathogen. we studied the persistence of genital herpes lesion-derived hsv-specific cd8+ ctl from three immunocompetent individuals with frequently recurring genital hsv-2 infection. all ctl clones were hsv-2 type specific and only one to three ... | 2000 | 10878394 |
| transfusion-associated infections with cytomegalovirus and other human herpesviruses. | of all human herpesviruses, human cytomegalovirus (hcmv) is the most significant cause of transfusion-associated (ta) morbidity and mortality. the problem of ta hcmv infection differs from that of other transfusion-transmitted infections in that only certain groups of patients require hcmv-free blood or blood components, i.e. seronegative pregnant women, premature infants of low birth weight who are born to seronegative mothers, seronegative recipients of allogeneic bone marrow transplants from ... | 2000 | 10878482 |
| herpesvirus dna detection in cerebral spinal fluid: differences in clinical presentation between alpha-, beta-, and gamma-herpesviruses. | to evaluate the role of 6 human herpesviruses (cytomegalovirus (cmv), epstein-barr virus (ebv), human herpesvirus-6 (hhv-6), herpes simplex virus (hsv) types 1 and 2 and varicella zoster virus (vzv)) in infections of the nervous system, cerebrospinal fluid (csf) samples from 662 patients with suspected viral aetiology to neurological symptoms were investigated for presence of herpesviral dna in a pcr-based study. of the 69 patients (2 patients had 2 herpesvirus dna detected in csf) who had herpe ... | 2000 | 10879592 |
| need for diagnostic screening of herpes simplex virus in patients with nongonococcal urethritis. | the prevalence of various microorganisms known to cause nongonococcal urethritis, including herpes simplex virus (hsv), was evaluated. the findings suggest that hsv can be a significant etiological agent in nongonococcal urethritis (ngu) and that the necessary laboratory investigations should be performed for all patients with clinical symptoms of ngu. | 2000 | 10880323 |
| comparison of a monoclonal antibody-blocking enzyme-linked immunoassay and a strip immunoblot assay for identifying type-specific herpes simplex virus type 2 serological responses. | detection of herpes simplex virus type 2 (hsv-2)-specific antibodies by a monoclonal antibody (mab)-blocking enzyme-linked immunoassay (eia) was compared with detection by a strip immunoblot assay (sia) in a sexually transmitted disease (std) clinic population. the study population consisted of 1,683 genitourinary medicine clinic attendees (582 women and 1,101 men). sera were tested for the presence of hsv-2 antibody by use of the blocking eia, in which binding of the mab ap-1 to hsv-2 glycoprot ... | 2000 | 10882665 |
| modulation of the immune response to dna vaccine by co-delivery of costimulatory molecules. | we have investigated methods for modulating immune responses, against herpes simplex virus (hsv), generated from dna vaccination by co-delivery of genes encoding costimulatory molecules. a strong delayed-type hypersensitivity (dth) reaction was induced in mice co-injected via the intradermal (i.d.) route with a eukaryotic expression plasmid encoding the cd80 molecule (pcd80) and a plasmid encoding the glycoprotein d of the hsv-2 (pgd). furthermore, when spleen cells from these mice were cultured ... | 2000 | 10886404 |
| structure-activity relationships of (e)-5-(2-bromovinyl)uracil and related pyrimidine nucleosides as antiviral agents for herpes viruses. | a series of (e)-5-(2-bromovinyl)uracil analogues and related nucleosides was synthesized, and their antiviral activities were evaluated. (e)-5-(2-bromovinyl)-2'-deoxy-l-uridine (l-bvdu, 2), 1-(beta-l-arabinofuranosyl)-(e)-5-(2-bromovinyl)uracil (l-bvau, 4), (e)-5-(2-bromovinyl)-1-(2-deoxy-2-fluoro-beta-l-ribofuranosyl)uracil (l-fbvru, 8) and (e)-5-(2-bromovinyl)-1-(2-deoxy-2-fluoro-beta-l-arabinofuranosyl)urac il (l-fbvau, 10) were synthesized via appropriate 5-iodouracil analogues from l-arabin ... | 2000 | 10891113 |
| [seroprevalence of type 2 herpes simplex virus in an adult population in the community of madrid]. | 2000 | 11153208 | |
| prevalence of antibodies to herpes simplex virus 2 among homosexual men either positive or negative for human immunodeficiency viruses in slovakia. | we determined the prevalence of antibodies to herpes simplex virus 2 (hsv-2, hsv-2 antibodies) in sera of homosexual men either positive for human immunodeficiency virus 1 (hiv-1, hiv+, a group of 27 sera) or negative for hiv-1 and hiv-2 (hiv-, a group of 52 sera) in slovakia. antibodies to hsv-2 glycoprotein g-2 (gg-2, gg-2 antibodies) were determined by a double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa) and immunoblot analysis. we found that 40% of hiv+ and 23% of hiv- ho ... | 2000 | 11155359 |
| how human immunodeficiency viruses and herpesviruses affect apoptosis. | eighty to hundred percent of patients positive for human immunodefficiency viruses 1 or 2 (hiv) may develop opportunistic viral infections. according to the national institute of health data, only in the usa the hiv patients are positive also for human cytomegalovirus (hcmv) in 25-40%, varicella-zoster virus (vzv) in 10%, herpes simplex viruses 1 and 2 (hsv-1 and hsv-2), and kaposi's sarcoma-associated herpesvirus (human herpesvirus 8, kshv, hhv-8) in 20%. hiv and herpesviruses express numerous ... | 2000 | 11155367 |
| high frequency of asymptomatic shedding of herpes simplex virus type 2 in african women. | 2000 | 11168043 | |
| expression of herpes simplex virus type 2 us3 affects the cdc42/rac pathway and attenuates c-jun n-terminal kinase activation. | although the us3 gene product of herpes simplex virus (hsv) has been identified as a serine/threonine protein kinase (pk), the functions are poorly understood. | 2000 | 11168588 |
| disinfection of eyelid specula with chlorhexidine gluconate (hibiclens) after examinations for retinopathy of prematurity. | the preferred method of cleaning eyelid specula between examinations for retinopathy of prematurity is unknown. a previous study showed that disinfection with 70% isopropyl alcohol swabs fails to eliminate viruses and bacteria from the specula. | 2000 | 10865315 |
| maternal intrauterine herpes simplex virus infection leading to persistent fetal vasculature. | herpes simplex virus can cause serious ocular and systemic disease in the neonate. the mode of transmission to the neonate is usually from the maternal birth canal to the fetus intrapartum; but much more rarely, hematogenous transplacental infection can affect the developing fetus months prior to birth. persistent fetal vasculature occurs when there is persistence of the fetal ocular vasculature, which normally regresses prior to birth. to our knowledge, we report the first case of serologically ... | 2000 | 10865323 |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| the antiviral activity exerted by vaccinia virus on the growth of herpes simplex virus in bs-c-1 cells. | the growth of herpes simplex virus type 2 (hsv-2) in bs-c-1 cells, was inhibited following super-infection with vaccinia virus. this inhibition was efficiently induced by both the intracellular mature virus (imv) form of vaccinia virus and the extracellular enveloped virus (eev), containing an additional external viral membrane. treatment of vaccinia imv with the detergents np-40, brij-58 or n-octyl-alpha-d-glucopyranoside, abolished its ability to inhibit the growth of hsv-2. ultraviolet irradi ... | 2000 | 10854666 |
| herpes simplex virus: the importance of asymptomatic shedding. | herpes simplex virus (hsv) is frequently shed after infection of the genital or perianal area. hsv shedding, as determined by culture, occurs on about 3% of days for immunocompetent women and men, and more for persons with hiv infection or if measured by polymerase chain reaction (pcr). most horizontal and vertical transmission of hsv occurs during unrecognized or asymptomatic shedding, and the majority of hsv-2-infected persons are unaware of their infection. many persons with 'asymptomatic' hs ... | 2000 | 10855766 |
| testing for type-specific antibody to herpes simplex virus--implications for clinical practice. | recently, assays that can distinguish between antibody to herpes simplex virus (hsv) types 1 and 2 have become available. these tests not only make it possible to better define infection in symptomatic patients and their sexual partners but also to identify asymptomatic infected individuals who may, nevertheless, be infectious. type-specific antibody tests for hsv have several potential applications. they have a clear role in helping to define the worldwide distribution and pattern of hsv infect ... | 2000 | 10855767 |
| peptide based enzyme-linked immunoassays for detection of anti-hsv-2 igg in human sera. | glycoprotein g of hsv-2 (gg2) and a peptide, corresponding to a previously recognised immunodominant epitope spanning residues 561-578 of the protein, were compared directly for type-specific serodiagnosis of hsv-2. the protein was affinity purified and obtained in a commercially available eia kit while the peptide, previously designated as peptide 55, was made as a multiple antigenic peptide. a panel of 100 characterised serum samples (60 hsv-2 positive, 20 hsv-1 positive and 20 hsv negative) w ... | 2000 | 10856753 |
| a monoclonal blocking eia for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in africa. | a competitive type-specific enzyme-linked immunosorbent assay (elisa) for herpes simplex virus type 2 (hsv-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein g-2. this assay has been validated for use for epidemiological studies using a large panel of sera collected in rural uganda and a panel of 143 sera characterised previously by western blotting, the 'gold standard' for hsv type-specific serology. this evaluation was found to have a sensitivity ... | 2000 | 10856754 |
| detection of human serum antibodies against type-specifically reactive peptides from the n-terminus of glycoprotein b of herpes simplex virus type 1 and type 2 by surface plasmon resonance. | a single-step surface plasmon resonance protocol for the detection of antibodies against herpes simplex virus type 1 and type 2 (hsv-1, hsv-2) in human sera was established using the biacore system. two peptides from corresponding segments of the n-terminus of hsv-1 and hsv-2 glycoprotein b (gb), i.e. peptide gb-1 (60-73) (gaaptgdpkpkknk) and peptide gb-2 (55-68) (spattkarkrktkk), were identified as immunogenic. employing both peptides as diagnostic antigens in the surface plasmon resonance assa ... | 2000 | 10856760 |
| synthesis and biological activities of new carbaacyclonucleosides and 1'-oxaacyclonucleosides related to clitocine. | we describe the synthesis of two series of acyclonucleosides:carbaacyclonucleosides and 1'-oxaacyclonucleosides which possess the same aglycone as clitocine 3 which is a natural nucleoside exhibiting interesting biological properties. these compounds have been obtained by condensation of 4-aminobutanol or 3-silyloxypropoxyamine with 4,6-dichloro-5-nitropyrimidine. structural modifications have been made on the heterocyclic base and the side chain to enhance their potential activity. all these co ... | 2000 | 10843493 |
| herpes simplex type 2 infections--an update. | 2000 | 10844903 | |
| a novel human gene similar to the protein kinase (pk) coding domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) codes for a serine-threonine pk and is expressed in melanoma cells. | the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is a multifunctional protein that contains a serine-threonine protein kinase (pk) activity (nelson, j. w., zhu, j. , smith, c. c., kulka, m., and aurelian, l. (1996) j. biol. chem. 271, 17021-17027). phylogenetic analyses indicated that icp10 pk belongs to a distinct subfamily of growth factor receptor serine-threonine pks that are characterized by their ability to function with a limited number of conserved cataly ... | 2000 | 10833516 |
| mitochondrial distribution and function in herpes simplex virus-infected cells. | in this study, mitochondria migrated to a perinuclear region in the cytoplasm in herpes simplex virus (hsv)-infected cells. hsv infection did not promote the expression of cytochrome c oxidase subunit 2 but did promote that of stress-responsive hsp60, both of which are known to be components of mitochondria. the levels of cellular atp and lactate and mitochondrial membrane potential were maintained for at least 6 h but decreased at the late stage of infection. it was also found that the ul41 and ... | 2000 | 10644838 |
| evaluation of an enzyme immunoassay system for measuring herpes simplex virus (hsv) type 1-specific and hsv type 2-specific igg antibodies. | mrl diagnostics has developed a dual enzyme immunoassay (eia) system that employs the recombinant herpes simplex virus (hsv) type-specific glycoproteins g1 (hsv1) and g2 (hsv2) to detect hsv type-specific igg antibodies. this system was evaluated using 155 consecutive sera previously tested in a conventional dual eia system (zeus) that employs multiple hsv1 and hsv2 proteins to detect type-common as well as type-specific antibodies. sera were also analyzed by western blot to determine the true h ... | 2000 | 10645979 |
| geographical differences in human herpesvirus 8 seroepidemiology: a survey of 1,201 individuals in asia. | since the discovery of human herpesvirus 8 (hhv8) as a contributory cause of kaposis sarcoma, the clinical role of this virus has been actively investigated. an understanding of hhv8 seroepidemiology is critical for the study of its pathogenesis within a specific environment. a sero-survey is described in taiwan of 1,201 individuals ranging in age from under 1 year to over 70. indirect immunofluorescence assay was used to determine antibody titers against both latent and lytic antigens of hhv8. ... | 2000 | 10630961 |
| herpes simplex type 2 infections--an update. | 2000 | 10635049 | |
| diagnosis and surveillance of herpes simplex virus infection of the central nervous system. | herpes simplex viruses (hsv) are responsible for neurological disorders that require rapid diagnostic methods and specific antiviral therapy. during 1997, 1431 cerebrospinal fluid samples (csf) collected from 1339 patients with neurological disorder presentations were processed for hsv detection. eleven patients were positive for hsv, seven presenting with encephalitis (6/7 due to hsv1) and 4 with aseptic meningitis (4/4 due to hsv2). the incidence of hsv encephalitis was 2.33 cases / 10(6) inha ... | 2000 | 10897065 |
| characterization of cell-cell fusion mediated by herpes simplex virus 2 glycoproteins gb, gd, gh and gl in transfected cells. | the mechanisms by which herpes simplex viruses (hsv) mediate fusion between their envelope and the plasma membrane during entry into cells, and between the plasma membranes of adjacent infected and uninfected cells to form multinucleated giant cells, are poorly understood. four viral glycoproteins (gb, gd, gh and gl) are required for virus-cell fusion, whereas these plus several others are required for cell-cell fusion (syncytium formation). a better understanding would be aided by the availabil ... | 2000 | 10900041 |
| synthesis and enantioselectivity of the antiviral effects of (r,z)-,(s,z)-methylenecyclopropane analogues of purine nucleosides and phosphoralaninate prodrugs: influence of heterocyclic base, type of virus and host cells. | a series of r and s enantiomers of 2-aminopurine methylenecyclopropane analogues of nucleosides was synthesized. two diastereoisomeric lipophilic phosphate prodrugs derived from r and s enantiomers of 2,6-diaminopurine analogue were also prepared. enantioselectivity (diastereoselectivity in case of prodrugs) of in vitro antiviral effects was investigated with human and murine cytomegalovirus (hcmv and mcmv, respectively), herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively), human ... | 2000 | 10901290 |
| inhibition of no production in macrophages by il-13 is counteracted by herpes simplex virus infection through tumor necrosis factor-alpha-induced activation of nk-kappa b. | interleukin (il)-13 is known to antagonize many interferon (ifn)-gamma-activated functions in macrophages and among these, nitric oxide (no) production. we have previously shown that this function of il-13 is reduced in herpes simplex virus type 2 (hsv-2)-infected macrophages. in the present study we show that il-13 and ifn-gamma are indeed produced during infection of balb/c mice with hsv-2. the lack of inhibitory function of il-13 in infected macrophages, which was not overcome even at very hi ... | 2000 | 10903807 |
| association between clinical disease activity and epstein-barr virus reactivation in ms. | to assess the potential significance of epstein-barr virus (ebv) reactivation in disease activity in ms patients. | 2000 | 10908887 |
| lfa-3 plasmid dna enhances ag-specific humoral- and cellular-mediated protective immunity against herpes simplex virus-2 in vivo: involvement of cd4+ t cells in protection. | adhesion molecules are important for cell trafficking and delivery of secondary signals for stimulation of t cells and antigen-presenting cells (apcs) in a variety of immune and inflammatory responses. adhesion molecules lymphocyte function-associated antigen (lfa)-1 and cd2 on t cells recognize intercellular adhesion molecule (icam)-1 and lfa-3 on apcs, respectively. recent studies have suggested that these molecules might play a regulatory role in antigen-specific immune responses. to investig ... | 2000 | 10915558 |
| construction, phenotypic analysis, and immunogenicity of a ul5/ul29 double deletion mutant of herpes simplex virus 2. | a number of studies have shown that replication-defective mutant strains of herpes simplex virus (hsv) can induce protective immunity in animal systems against wild-type hsv challenge. however, all of those studies used viruses with single mutations. because multiple, stable mutations provide optimal levels of safety for live vaccines, we felt that additional mutations needed to be engineered into a candidate vaccine strain for hsv-2 and genital herpes. we therefore isolated an hsv-2 strain with ... | 2000 | 10933704 |
| [typing of herpes simplex virus in patients with uveitis]. | to examine the type of herpes simplex virus (hsv) in cases with uveitis. | 2000 | 10934865 |
| qsar and molecular graphics analysis of n2-phenylguanines as inhibitors of herpes simplex virus thymidine kinases. | a quantitative structure-activity relationship study of n2-(substituted)-phenylguanines (phg) as inhibitors of herpes simplex virus thymidine kinase (hsv tk) was performed. the activity of a set of phg derivatives were analyzed against the thymidine kinase of herpes simplex virus types 1 (hsv1 tk) and 2 (hsv2 tk). classic and calculated physicochemical parameters were included in the analysis. the results showed that there is an important difference in the activity of the meta substituted phg de ... | 2000 | 10935205 |
| rapid strip assay for detection of anti-herpes simplex virus antibodies: application to prediction of varicella-zoster virus reactivation in patients with acute peripheral facial palsy. | varicella-zoster virus (vzv) reactivation causes acute peripheral facial palsy in the majority (88%) of patients who lack anti-herpes simplex virus (hsv) antibodies, suggesting that an absence of anti-hsv antibodies is a reliable serological marker for the diagnosis of vzv reactivation in patients who are diagnosed initially as idiopathic peripheral facial palsy (bell's palsy) [furuta et al., 2000] clinical infectious diseases]. a simple and rapid immunoassay for detection of anti-hsv antibodies ... | 2000 | 10935986 |
| herpes simplex virus-specific t cells infiltrate the cornea of patients with herpetic stromal keratitis: no evidence for autoreactive t cells. | herpetic stromal keratitis (hsk) is a t-cell-mediated inflammatory disease initiated by a herpes simplex virus (hsv) infection of the cornea. recently, studies in the hsk mouse model have shown that the immunopathogenic t cells are directed against the hsv protein ul6 cross-reacting with an unknown corneal autoantigen. whether this type of autoimmunity plays a role in human hsk was analyzed. | 2000 | 10937573 |
| seroepidemiologic study of herpes simplex virus type 2 and cytomegalovirus among young adults in northern jordan. | blood samples were randomly collected from 360 males and 390 females among apparently healthy university students aged 18-24 years and tested for herpes virus type 2 (hsv-2) and cytomegalovirus (cmv) antibodies. the prevalence of hsv-2 seropositivity was 52.8% for males and 41.5% for females as detected by elisa. on the other hand, the prevalence of cmv seropositivity was 75.6% in males and 77.2% in females. the high percentage of seropositivity in our study is most probably due to the crowded l ... | 2000 | 10939038 |
| changing trends in genital herpes simplex virus infection in bergen, norway. | to document the proportion of each herpes simplex virus (hsv) type in genital hsv infection and changes over time during a 10 year period. | 2000 | 10949236 |
| a model of the transmission and control of genital herpes. | mathematical modeling of herpes simplex virus type 2 transmission can provide insight into the behavior of the epidemic and the effects of control measures. | 2000 | 10949427 |