Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| herpes simplex virus dna in amniotic fluid without neonatal infection. | twenty-one pregnant women were studied to determine the distribution of herpes simplex virus (hsv): 10 had symptomatic genital herpes, including 1 with primary cervical hsv infection, and 11 had asymptomatic genital herpes. samples from vesicles, the cervix, and amniotic fluid (af) were analyzed with 2 separate polymerase chain reaction (pcr) tests and with viral culture. for newborns, clinical examinations and pharyngeal hsv cultures were performed, and cord blood igm antibodies to hsv were mea ... | 2000 | 10671342 |
| viral causes of the acute retinal necrosis syndrome. | the primary goal of this study was to determine the viral cause of the acute retinal necrosis syndrome in 28 patients (30 eyes). a secondary goal was to investigate possible associations between viral cause and patient age, and viral cause and central nervous system disease. | 2000 | 10682968 |
| immunologic strategies for herpes vaccination. | 2000 | 10683051 | |
| immunologic strategies for herpes vaccination. | 2000 | 10683052 | |
| genital herpes and public health: addressing a global problem. | genital herpes can be caused by herpes simplex virus 2 (hsv-2) or, less commonly, by herpes simplex virus 1 (hsv-1). with a seroprevalence of antibodies to hsv-2 of 22% in the general population, genital herpes is 1 of the 3 most prevalent sexually transmitted diseases (stds) in the united states. a central issue in the public health problem of genital herpes is the high proportion of genital hsv infections that are unrecognized by both patients and clinicians. persons who are hsv-2 seropositive ... | 2000 | 10683059 |
| the cyclohexene ring system as a furanose mimic: synthesis and antiviral activity of both enantiomers of cyclohexenylguanine. | both enantiomers of cyclohexenylguanine were synthesized in a stereospecific way starting from the same starting material: r-(-)-carvone. both compounds showed potent and selective anti-herpesvirus activity (hsv-1, hsv-2, vzv, cmv). the binding of both cyclohexene nucleosides in the active site of hsv-1 thymidine kinase was investigated, and a model for the binding of both enantiomers is proposed. the amino acids involved in binding of the optical antipodes are the same, but the interaction ener ... | 2000 | 10691698 |
| is placental tissue protein 17b/tip47 a new factor in cervical cancer genesis? | we identified novel members of the placental tissue protein 17 (pp17) protein family which consists of different-size variants of pp17; cdnas of pp17a and pp17b variants have also been cloned and sequence analyzed. by western-blot analysis in cervical carcinoma tissues we found overexpression of pp17b. compared to healthy controls a mean five-fold increase in the amount of pp17b was also detected in the sera of untreated cervical carcinoma patients, which declined after radical operations. in ou ... | 1999 | 10697545 |
| etiology of sexually transmitted infections among street-based female sex workers in dhaka, bangladesh. | an etiological study of sexually transmitted infections (stis) was conducted among female sex workers (fsws) in dhaka, bangladesh. endocervical swab and blood samples from 269 street-based fsws were examined for neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis as well as for antibodies to treponema pallidum and herpes simplex virus 2 (hsv-2). sociodemographic data and data regarding behavior were also collected. a total of 226 of the 269 fsws (84%) were positive for the st ... | 2000 | 10699032 |
| therapeutic vaccination against hsv-2: influence of vaccine formulation on immune responses and protection in mice. | therapeutic immunisation may represent a means of influencing viral infections that persist in the host by modulating the nature or level of host immunity. to assess the influence of the form of the antigenic stimulus on immunity to type-2 herpes simplex virus (hsv-2), mice pre-infected with sublethal doses of hsv-2 were immunised with various hsv-2 vaccine formulations prior to challenge infection with heterologous hsv-1. measurements of interleukin-2 (il-2), interleukin-4 (il-4) and interferon ... | 2000 | 10699326 |
| dna immunization utilizing a herpes simplex virus type 2 myogenic dna vaccine protects mice from mortality and prevents genital herpes. | a gene transfer vector for dna immunization was developed in which the promoter was derived from the murine muscle creatine kinase (mck) gene; a gene expressed only in differentiated skeletal muscle. in vitro, we observed high-level, but unrestricted, gene expression from the cytomegalovirus (cmv) promoter unlike expression from the mck promoter which was weak but restricted to myofibers. a myogenic dna vaccine (mdv) that encoded the glycoprotein d gene from herpes simplex virus type-2 (hsv-2) w ... | 2000 | 10699332 |
| [herpes simplex virus type 1 and 2 in switzerland]. | a worldwide increase in the incidence of genital herpes infections has been described in recent years. transmission of the herpes simplex virus type 2 (hsv-2) by asymptomatic seropositive subjects is considered to be a relevant mode of infection. the seroprevalence of hsv-2 varies considerably between different populations. in europe, data are scarce and the epidemiological situation in switzerland is unknown. in 1997 we performed serological examinations in 151 adult volunteers (87% between 20 ... | 2000 | 10701231 |
| high prevalence of herpes simplex virus type 2 in acute retinal necrosis syndrome associated with herpes simplex virus in japan. | to determine the type of herpes simplex virus in acute retinal necrosis syndrome associated with herpes simplex virus. | 2000 | 10704570 |
| enhancement of antiherpetic activity of cytarabine by hydroxyurea in human embryonic skin-muscle fibroblasts. | pharmacological induction of low deoxyribonucleoside triphosphate (dntp) levels is a novel approach to combine inhibition of virus replication. in accordance with this concept, the alteration of antiherpes activity of cytarabine (arac) in combination with hydroxyurea (hu), an inhibitor of ribonucleotide reductase, was studied. the results were confirmed by virus yield assays in human embryonic skin-muscle fibroblasts (hesmf). it was demonstrated that hu significantly enhanced the antiviral activ ... | 2000 | 10705616 |
| necrotizing ulcerative stomatitis in human immunodeficiency virus-seropositive individuals: a review of the histopathologic, immunohistochemical, and virologic characteristics of 18 cases. | the purpose of this retrospective study was to delineate the histopathologic, immunohistochemical, and virologic characteristics of 18 cases of necrotizing ulcerative stomatitis. | 2000 | 10710458 |
| antibody responses, cytokine levels and protection of mice immunised with hsv-2 antigens formulated into nisv or iscom delivery systems. | the immunogenicity of a type 2 herpes simplex virus (hsv-2) antigen preparation following its formulation into immunostimulating complexes (iscoms) or non-ionic surfactant vesicles (nisv) was investigated in a murine model. the immune responses induced by each formulation were characterised by antigen specific total and subclass serum responses, and by lymphocyte proliferation and cytokine (interleukin-2 (il-2), interleukin-4 (il-4) and interferon-gamma (ifn-gamma)) production by in vitro restim ... | 2000 | 10715522 |
| anti-herpes simplex virus activities of crude water extracts of thai medicinal plants. | a number of thai medicinal plants, recommended as remedies for herpesvirus infection and have been used in primary health care were investigated for their intracellular activities against herpes simplex viruses (hsv). centella asiatica l., maclura cochinchinensis cornor, and mangifera indica l. contained both anti-hsv-1 and -2 activities, as determined by plaque inhibition assay. an inhibition of the production of infectious hsv-2 virions from infected vero cells could also be demonstrated. comb ... | 2000 | 10715843 |
| anti-herpes simplex virus component isolated from maclura cochinchinensis. | the powerful anti-herpes simplex virus (hsv) activity of maclura cochinchinensis in vitro prompted us to carry out biologically-guided separation of the active component(s). ethyl acetate and methanol extracts exhibited anti-hsv-2 activity at ec50 values of 38.5 micrograms/ml and 50.8 micrograms/ml, respectively. although petroleum ether extract was inactive, a chloroform extract was too toxic to the test cell culture to perform the test. biologically-guided chromatographic separation of the eth ... | 2000 | 10715844 |
| the cause of invasive cervical cancer could be multifactorial. | cancer of the cervix is the third most common cancer among women worldwide, with incidence rates ranging from 3.8 per 100,000 women per year in israel to 48.2 per 100,000 per year in colombia. epidemiologic and clinical data suggest that human papillomaviruses, especially hpv-16 and hpv-18, play the major role in the etiology of cervical cancer. however, many investigators acknowledge that hpv is neither necessary nor sufficient in the etiology of cervical cancer and that a multifactorial etiolo ... | 2000 | 10721464 |
| antiviral properties of a series of 1,6-naphthyridine and 7, 8-dihydroisoquinoline derivatives exhibiting potent activity against human cytomegalovirus. | a series of 1,6-naphthyridine (l. chan, h. jin, t. stefanac, j. f. lavallee, g. falardeau, w. wang, j. bedard, s. may, and l. yuen, j. med. chem. 42:3023-3025, 1999) and isoquinoline (l. chan, h. jin, t. stefanac, w. wang, j. f. lavallee, j. bedard, and s. may, bioorg. med. chem. lett. 9:2583-2586, 1999) analogues exhibiting a high level of anti-human cytomegalovirus (hcmv) activity were investigated in a series of studies aimed at better understanding the mechanism of action of some representat ... | 2000 | 10722493 |
| immunisation with phage displaying peptides representing single epitopes of the glycoprotein g can give rise to partial protective immunity to hsv-2. | filamentous phage displaying peptides representing single epitopes of the glycoprotein g of hsv-2 (gg2) were used as immunogens via the subcutaneous route in balb/c mice without additional adjuvant. the phage were isolated from a random phage peptide display library and contain 15-mer peptide inserts that mimic epitopes of gg2. in each case, an antibody response to gg2 was generated that was dependent on the dose of phage administered and on the presence of the peptide insert. phage displaying e ... | 2000 | 10725197 |
| glycoprotein g of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies. | serological diagnosis of herpes simplex virus (hsv) infections requires assays based on antigens that expose type-specific determinants. this study was designed to outline the b-cell epitopes of the type-specific glycoprotein g-1 (gg-1) of hsv type 1 (hsv-1), by investigating the reactivity of human anti-gg-1 antibodies, purified from 21 hsv-1-isolation-proven patient sera, to cellulose-bound synthetic peptides spanning the entire gg-1 sequence. the epitope mapping demonstrated that these antibo ... | 2000 | 10725430 |
| reactivation of genital herpes simplex virus type 2 infection in asymptomatic seropositive persons. | most persons who have serologic evidence of infection with herpes simplex virus (hsv) type 2 (hsv-2) are asymptomatic. historically, it has been assumed that these persons have less frequent viral reactivation than those with symptomatic infection. | 2000 | 10727588 |
| herpes simplex virus infections. new treatment approaches make early diagnosis even more important. | the herpes simplex viruses hsv-1 and hsv-2 cause problems for millions of people worldwide. infections range from simple cold sores and fever blisters to severe central nervous system disorders. development of effective antiviral medications has made prompt recognition important in primary care practice. appropriate therapy can significantly reduce both medical and psychosocial ramifications of herpes infections and can greatly improve the quality of life for many patients. | 2000 | 10728143 |
| herpes simplex virus virion host shutoff (vhs) activity alters periocular disease in mice. | during lytic infection, the virion host shutoff (vhs) protein of herpes simplex virus (hsv) mediates the rapid degradation of rna and shutoff of host protein synthesis. in mice, hsv type 1 (hsv-1) mutants lacking vhs activity are profoundly attenuated. hsv-2 has significantly higher vhs activity than hsv-1, eliciting a faster and more complete shutoff. to examine further the role of vhs activity in pathogenesis, we generated an intertypic recombinant virus (kosv2) in which the vhs open reading f ... | 2000 | 10729135 |
| [herpes simplex virus types 1 and 2 (hsv-1 and hsv-2)]. | herpes simplex virus (hsv) infections are prevalent worldwide. hsv are characterized by their ability to establish and maintain latent infections that can be reactivated. various clinical presentations of hsv infections are described. mostly asymptomatic, these infections may become life-threatening when occurring in neonates or when infecting the central nervous system. accurate diagnosis of hsv infections is important and pcr is the most sensitive technique for detecting hsv. type-specific ser ... | 1999 | 10731803 |
| polyherbal formulations with wide spectrum antimicrobial activity against reproductive tract infections and sexually transmitted pathogens. | recent reports indicate high incidence of genital infections, most of which are sexually transmitted. although specific drugs and antibiotics are available for some, a safe spermicidal formulation with wide spectrum antimicrobial action would be a desirable addition to the presently available spermicides. | 2000 | 10735590 |
| hydrogels containing monocaprin prevent intravaginal and intracutaneous infections with hsv-2 in mice: impact on the search for vaginal microbicides. | hydrogel formulations containing the 1-monoglyceride of capric acid (monocaprin) possess potent in vitro microbicidal activity against hiv and hsv, chlamydia trachomatis and neisseria gonorrhoeae. these formulations were studied to determine whether they prevent intracutaneous and intravaginal infections of mice with hsv-2, a virus that is in vitro as sensitive to the virucidal action of the compound as is hiv. in mice intravaginal infection with hsv-2 and the associated mortality was prevented ... | 2000 | 10745241 |
| neonatal herpes simplex virus type ii. | this description of a tragic case of infection with neonatal herpes simplex virus type ii is presented to alert professional nurses to the fact that this disease is sometimes difficult to recognize, but must be considered when a newborn has any unusual symptoms. neonatal herpes simplex type ii may have a slow and insidious onset, and be masked by many other symptoms. even though the virus might be present in a newborn, the symptoms might not be apparent until several days after birth; this is a ... | 2000 | 10748589 |
| preclinical evaluation of "whole" cell vaccines for prophylaxis and therapy using a disabled infectious single cycle-herpes simplex virus vector to transduce cytokine genes. | the development of genetically modified "whole" tumor cell vaccines for cancer therapy relies on the efficient transduction and expression of genes by vectors. in the present study, we have used a disabled infectious single cycle-herpes simplex virus 2 (disc-hsv-2) vector constructed to express cytokine or marker genes upon infection. disc-hsv-2 is able to infect a wide range of tumor cells and efficiently express the beta-galactosidase reporter gene, granulocyte-macrophage colony-stimulating fa ... | 2000 | 10749137 |
| herpes simplex virus type-1 and -2 pathogenesis is restricted by the epidermal basement membrane. | murine flank scarification with hsv-1 and -2 results in primary lesions at the site of inoculation within three days and lesions at secondary sites within four days. the severity of the infection can be given a numerical value or "score" which is derived from the number and size of these lesions. using this model, we investigated the role of the epidermal basement membrane in hsv pathogenesis. we exposed murine epidermis to 5 x 10(4) plaque forming units of hsv-1 and -2, which by day 8 produced ... | 2000 | 10752560 |
| herpes simplex virus ul17 protein is associated with b capsids and colocalizes with icp35 and vp5 in infected cells. | a previous study using a mutant lacking the ul17 gene has suggested that the ul17 protein of herpes simplex virus type 1 (hsv-1) is required for the cleavage/packaging of viral dna. in this study, we have raised a rabbit polyclonal antiserum which specifically reacted with the ul17 protein which has an apparent molecular mass of 78-kda in the lysates of hsv types 1- and 2-infected vero cells. western blot analysis of intracellular capsids demonstrates that the ul17 protein was associated with b ... | 2000 | 10752563 |
| hla-dq tetramers identify epitope-specific t cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells. | ag-specific cd4+ t cells are present in peripheral blood in low frequency, where they undergo recruitment and expansion during immune responses and in the pathogenesis of numerous autoimmune diseases. mhc tetramers, which constitute a labeled mhc-peptide ligand suitable for binding to the ag-specific receptor on t cells, provide a novel approach for the detection and characterization of such rare cells. in this study, we utilized this technology to identify hla dq-restricted ag-specific t cells ... | 2000 | 10754321 |
| rapid detection of glycoprotein g gene for the diagnosis and typing of herpes simplex virus infection in genital herpes. | to develop a new, rapid, and convenient technique for the diagnosis and typing of herpes simplex virus (hsv) in genital herpes (gh). | 1999 | 10754943 |
| british co-operative clinical group national survey on diagnostic issues surrounding genital herpes. mssvd special interest group on genital herpes and the british co-operative clinical group. | to investigate the current use of diagnostic methods for genital herpes simplex virus (hsv) infection, to determine how information from these tests influences clinical practice, and to identify areas for future guideline development within genitourinary medicine (gum) clinics in the united kingdom. | 1999 | 10754945 |
| detection of herpes simplex virus dna in maternal breast milk by in situ hybridization with tyramide signal amplification. | in this study we investigated the prevalence of herpes simplex virus 1 (hsv-1) and 2 (hsv-2) in maternal breast milk using the technique of in situ hybridization combined with the sensitive detection system of tyramide signal amplification. breast milk samples were collected from both breasts of 34 puerperals 4-5 days after delivery. hsv dna was detected in 16 out of 34 examined breast milk specimens. hsv dna was found in the milk of both breasts in 10 cases (62.5%), in that of left breast in 4 ... | 1999 | 10757038 |
| synthesis and antiviral activity of acyclovir-5'-(phenyl methoxy alaninyl) phosphate as a possible membrane-soluble nucleotide prodrug. | we describe a synthesis of acyclovir-5'-(phenyl methoxy alaninyl) phosphate (2) from acyclovir (1). this compound was designed to act as a lipophilic, membrane-soluble prodrug of the free nucleotide. however, the biological activities of this derivative against a range of viruses indicated poor intracellular phosphate delivery, in marked contrast to the earlier successful delivery of several dideoxy anti-hiv nucleotides. | 2000 | 10762044 |
| use of herpes simplex virus (hsv) type 1 iscoms 703 vaccine for prophylactic and therapeutic treatment of primary and recurrent hsv-2 infection in guinea pigs. | the effect of subunit vaccination on the incidence and severity of primary and recurrent genital herpes was investigated in the female guinea pig model of herpes simplex virus (hsv) type 2 genital infection. after prophylactic immunization with zwitterionic detergent-solubilized hsv-1 glycoproteins formulated with alhydrogel or as immunostimulating complex particles, significant reductions in the incidence and severity of primary herpetic illness were observed in both vaccinated groups compared ... | 2000 | 10762560 |
| herpes simplex virus type 1 as a cause of genital herpes: impact on surveillance and prevention. | this study compared characteristics of patients who had herpes simplex virus (hsv) type 1 with characteristics of patients who had hsv-2, by use of data from a cross-sectional analysis. data were collected in an urban sexually transmitted diseases clinic from patients who had positive genital hsv cultures. overall, 17.1% (95% confidence interval [ci], 14.9%-19.3%) of 1145 genital hsv isolates obtained during 1993-1997 were hsv-1. the proportion of hsv-1 among initial genital herpes infections wa ... | 2000 | 10762576 |
| characterization of an acyclovir-resistant herpes simplex virus type 2 strain isolated from a premature neonate. | acyclovir resistance is not a recognized problem among neonates with perinatal herpes simplex virus (hsv) infection. a premature newborn with neurocutaneous hsv infection was treated for 21 days with acyclovir. disseminated disease recurred 8 days later. a recurrent isolate was resistant to acyclovir and lacked thymidine kinase activity on the basis of a frameshift mutation in the thymidine kinase (tk) gene. compared with the sensitive isolate obtained during primary infection, replication of th ... | 2000 | 10762577 |
| impact of antivirals and emergence of drug resistance: hsv-2 epidemic control. | genital herpes, caused by herpes simplex virus type-2 (hsv-2), affects more people world-wide than any other sexually transmitted disease (std). antivirals are effective in decreasing the duration of symptoms and in reducing viral shedding; however, currently antiviral usage is extremely low. increased usage of antivirals would have a beneficial epidemic-level effect (due to the decreased transmission of drug-sensitive strains) as well as potentially a detrimental epidemic-level effect (if drug- ... | 2000 | 10763542 |
| susceptibility of clinical strains of herpes simplex virus to three nucleoside analogues. | the susceptibility of clinical isolates of herpes simplex virus 1 (hsv1) (58 strains) and 2 (hsv2) (17 strains) from the centre hospitalier et universitaire de nancy to three nucleoside analogues was compared by the dye uptake method. as expected, all strains of hsv2 were resistant to brovavir or sorivudine. aciclovir and penciclovir activities were comparable; 2 strains of hsv1 were resistant to these two compounds. four strains isolated from immunocompromised patients gave different results wi ... | 2000 | 10765035 |
| antiviral activity of ganciclovir elaidic acid ester against herpesviruses. | a fatty acid derivative of ganciclovir (gcv), elaidic acid ganciclovir (e-gcv), has been evaluated for its inhibitory activity against laboratory and clinical strains of herpes simplex type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster virus (vzv) and human cytomegalovirus (hcmv) in human embryonic lung fibroblasts. gcv, cidofovir, acyclovir (acv), brivudin (bvdu) and foscarnet (pfa) were included as reference compounds. the viruses studied were wild-type, thymidine kinase-deficient (tk(-)) and ... | 2000 | 10771080 |
| synthesis and antiviral activities of enantiomeric 1-[2-(hydroxymethyl) cyclopropyl] methyl nucleosides. | cyclopropyl carbocyclic nucleosides have been synthesized from the key intermediate 2 which was converted to the mesylated cyclopropyl methyl alcohol 3. condensation of compound 3 with various purine and pyrimidine bases gave the desired nucleosides. all synthesized nucleosides were evaluated for antiviral activity and cellular toxicity. among them adenine 22 and guanine 23 derivatives showed moderate antiviral activity against hiv-1 and hbv. none of the other compounds showed any significant an ... | 2000 | 10772713 |
| efficacy of topical acyclovir monophosphate, acyclovir, or penciclovir in orofacial hsv-1 infections of mice and genital hsv-2 infections of guinea pigs. | the purpose of these studies was to compare the efficacy of acyclovir monophosphate (acvmp), acyclovir (acv), or penciclovir (pcv) against hsv-1 in an orofacial infection of mice and against acv sensitive and resistant genital hsv-2 infections of guinea pigs. treatment was initiated 24, 48, or 72 hours post inoculation with 5% acvmp, 5% acv (zovirax) or 1% pcv (denavir). in all experiments, similar efficacy was obtained for acvmp and acv, whereas pcv was considerably less effective. | 2000 | 10772730 |
| [the functions of alpha herpesvirus gene products]. | herpes simplex virus type 1(hsv-1), type 2(hsv-2) and varicella-zoster virus (vzv) belong to alphaherpesvirus family. these herpesviruses have large dna genomes which contain at least 69 genes. these viral genes are divided into two groups based on whether they are essential or dispensable for virus growth in cell culture. the essential gene products include transcriptional factor, viral dna replication, dna cleavage/packaging and virion components, while the dispensable gene products include de ... | 2000 | 10774196 |
| [seroepidemiology of alphaherpesviruses]. | the seroepidemiology of herpes simplex virus (hsv) type-1 and -2 was studied in different japanese populations, by applying hsv gg1 and gg2 type-specific antibody assays. hsv-1 infections correlated mostly with age and was widely prevalent among subjects over 40 years old. hsv-2 prevalence varied greatly among subgroups defined by sexual activity and were associated with risk behaviors, from 80% among prostitutes to 7% among pregnant women. since hsv-1 infection during childhood has been decreas ... | 2000 | 10774202 |
| [alpha-herpesvirus infection in the central nervous system]. | herpes simplex virus type 1 (hsv1) encephalitis is commonly observed, but hsv1 myelitis is rare. on the other hand, hsv2 is common etiologic agent for ascending necrotizing myelitis associated with diabetes mellitus, malignant tumors, aids and immunocompromised hosts. it is suggested that latent infection of the virus of root ganglia or primary infected virus is possibly followed by spread of infection to the central nervous system, particularly in immunocompromised patients. in this report, pat ... | 2000 | 10774207 |
| [alpha-herpes virus infections--male genital herpes]. | in japan, male genital herpes is the third popular male std, the most popular one being gonococcal infection and the second chlamydial infection. as specific clinical findings, superficial ulcer lesions with pain are formed in the genital area, especially in the prepuce. after hsv infection in genital mucocutaneous sites, viral particles are transported to the neurons. the state of subsequent hsv infection from external genitalia to the neurons is known as latent infection. 76% of our cases of t ... | 2000 | 10774209 |
| [sexually transmitted diseases of alpha herpes virus in women]. | among two alpha herpes viruses, herpes simplex virus(hsv) and varicella zoster virus(vzv), hsv infects genital sites and is frequently transmitted by sexual contact while vzv has quite different mode of transmission and rarely infects genital site except herpes zoster at the vulva. genital herpes is the second and the third leading cause of stds in women and men respectively. while 90% of male genital herpes was caused by hsv-2, 55% of female genital herpes was by hsv-2 and the remaining 45% by ... | 2000 | 10774210 |
| [ophthalmic area]. | although herpes simplex virus type 1 or herpes zoster virus is generally considered to cause ocular infection among alpha herpesviruses, herpes simplex virus type 2(hsv-2) retinitis has been recently reported. acute retinal necrosis(arn) syndrome is associated with herpesviruses, but the mechanisms remain unclear. the representative ocular infection by alpha herpesviruses is herpes simplex virus type 1(hsv-1) keratitis. the clinical types, ocular symptoms and therapy are reviewed in herpetic ocu ... | 2000 | 10774213 |
| risk factors for pre-cancerous lesions of the cervix. | studies on risk factors for pre-cancerous lesions of the uterine cervix have shown strong association with sexual practice. women with multiple sexual partners and intercourse at early age are at high risk. a role of male partners in further enhancing the risk has been identified. all these support the hypothesis relating to a sexually transmissible aetiological agent. an extensive review of the literature on the risk factors for pre-cancerous lesions of cervix has been carried out. the risk fac ... | 2000 | 10777005 |
| synthesis of 4'-substituted nucleosides and their biological evaluation. | 4'-c-ethynyl-beta-d-arabino- and 4'-c-ethynyl-beta-d-2'-deoxy-ribo-pentofuranosyl pyrimidines were synthesized. most of these 4'-ethynyl nucleosides showed interesting biological activities. | 1999 | 10780412 |
| fast and type-specific analysis of herpes simplex virus types 1 and 2 by rapid pcr and fluorescence melting-curve-analysis. | diagnosis of central nervous system (cns) infection with herpes simplex virus (hsv) requires sensitive and rapid techniques. pcr therefore is considered to be the diagnostic gold standard in these cases. however, current pcr protocols are time-consuming and labor-intensive. in addition, the need for post-amplification manipulations increases the risk of laboratory contaminations with amplified products. in order to improve conventional pcr techniques we compared our current semiautomated hsv-pcr ... | 2000 | 10782393 |
| a comparative multi-centre study of the efficacy of propolis, acyclovir and placebo in the treatment of genital herpes (hsv). | ninety men and women with recurrent genital hsv type 2 participated in a randomized, single-blind, masked investigator, controlled multi-centre study comparing the efficacy of ointment of canadian propolis containing natural flavonoids with ointments of acyclovir and placebo (vehicle) on healing ability and capacity to remedy symptoms. thirty individuals were randomized to each group. treatment was intended to start in the blister phase. all participants had hsv type 2 isolated, confirmed by ser ... | 2000 | 10782483 |
| etiology of microglial nodules in brains of patients with acquired immunodeficiency syndrome. | microglial nodules associated with opportunistic and hiv-related lesions are frequently found in the brains of aids patients. however, in many cases, the causative agent is only presumptively suspected. we reviewed 199 brains of aids patients with micronodular lesions to clarify their etiology by immunohistochemistry (to toxoplasma gondii, cytomegalovirus, herpes simplex virus i/ii, varicella zoster virus and hiv-p24 core protein), pcr (for herpetic viruses and mycobacterium tuberculosis) and el ... | 2000 | 10786996 |
| zinc salts inactivate clinical isolates of herpes simplex virus in vitro. | using a standard plaque assay and clinical isolates of herpes simplex virus (hsv), we have tested the ability of zinc salts to inactivate hsv. virus was treated by incubation at 37 degrees c with zinc salts in morpholinepropanesulfonic acid-buffered culture medium and was then diluted and plated onto cv-1 cells for detection and quantitation of remaining infectious virus. of 10 randomly chosen clinical isolates (five hsv type 1 [hsv-1] isolates and five hsv-2 isolates), seven were inactivated >9 ... | 2000 | 10790094 |
| rapid phenotypic characterization method for herpes simplex virus and varicella-zoster virus thymidine kinases to screen for acyclovir-resistant viral infection. | a rapid phenotypic screening method for herpes simplex virus (hsv) and varicella-zoster virus (vzv) thymidine kinase (tk) genes was developed for monitoring acyclovir-resistant viruses. this method determines the biochemical phenotype of the tk polypeptide, which is synthesized in vitro from viral dna using a procedure as follows. the tk gene of each sample virus strain is amplified and isolated under the control of a t7 promoter by pcr. the pcr products are transcribed with t7 rna polymerase an ... | 2000 | 10790110 |
| interferon-gamma up-regulates intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 and recruits lymphocytes into the vagina of immune mice challenged with herpes simplex virus-2. | lymphocyte recruitment into tissues involves interactions between adhesion molecules on vascular endothelial cells and corresponding ligands on the lymphocyte surface. in the present study we investigated the expression of four endothelial addressins in the vagina and their possible up-regulation by interferon-gamma (ifn-gamma) in immune mice after vaginal challenge with herpes simplex virus type 2. the adhesion molecules intercellular adhesion molecule-1 (icam-1) and vascular cell adhesion mole ... | 2000 | 10792501 |
| immune protection against hsv-2 in b-cell-deficient mice. | the role of antibody in protection of the vaginal mucosa and sensory ganglia against hsv-2 infection was examined using hsv- immune, b-cell-deficient mumt mice. significantly higher virus titers were detected in the vaginal mucosae of immune mumt mice compared to immune c57bl/6j mice 24 h after hsv-2 rechallenge. however, virus was rapidly cleared in immune mumt mice, and the infection was resolved with only a 2-day delay. passive transfer of immune serum to immune mumt mice prior to rechallenge ... | 2000 | 10793004 |
| molecular epidemiology of herpes simplex virus type 1 genital infection in association with clinical manifestations. | the antigenic types of herpes simplex virus (hsv), hsv-1 and hsv-2 are considered to be the etiology of genital herpes. symptoms of primary hsv-1 and hsv-2 genital infections are similar, however, recurrence of the infection is less frequent after the hsv-1-related genital infection. we determined genotypes of 79 hsv-1 strains isolated from genital lesions in women (43 from primary and 36 from recurrent infections), by analyzing restriction fragment length polymorphism of the hsv-1 strains. each ... | 2000 | 10795518 |
| herpes simplex virus type 2 infection of macrophages impairs il-4-mediated inhibition of no production through tnf-alpha-induced activation of nf-kappab. | nitric oxide (no) production in macrophages by the interferon (ifn)-gamma-inducible no synthase has been shown to play a role in clearance of viral infections. we have previously shown that ifn-gamma-induced no production is augmented by herpes simplex virus type 2 (hsv-2) infection through autocrine tumour necrosis factor (tnf)-alpha secretion and is inhibited by interleukin (il)-4. here we investigated the effect of hsv-2 infection on the inhibitory function of il-4. virus infection of mouse j ... | 2000 | 10795524 |
| evaluation of the inactivation of infectious herpes simplex virus by host-defense peptides. | a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide microplate assay was adapted to screen for the ability of 20 host-defense peptides to inactivate herpes simplex virus type 1 and type 2. the procedure required minimal amounts of material, was reproducible, and was confirmed with standard antiviral testing techniques. in screening tests, with the exception of melittin, a highly cytotoxic and hemolytic peptide found in bee venom, the alpha-helical peptides in our test panel (magainins ... | 2000 | 10795591 |
| evaluation of an enzyme-linked viral inducible system for the rapid detection of herpes simplex virus. | a commercial enzyme-linked viral inducible system (elvis hsv; biowhittaker, usa) was evaluated in comparison with the spin-amplified tube cell culture (satcc) method for the rapid detection of herpes simplex virus in 1007 clinical specimens. a total of 91 (9%) specimens were positive by satcc. the sensitivity, specificity, positive predictive value, and negative predictive value of elvis was 88%, >99%, 99%, and 99%, respectively. herpes simplex virus was detected sooner by elvis than by satcc in ... | 2000 | 10795600 |
| seroprevalence of herpes simplex virus type 1 and type 2 in selected german populations-relevance for the incidence of genital herpes. | this study was carried out to determine the prevalence of antibodies to herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) in selected german populations, such as blood donors, hospital patients, and human immunodeficiency virus (hiv)-seropositive individuals. serum samples collected between 1996 and 1998 were tested by enzyme immunoassays using monoclonal antibody-selected native gg1 and gg2 as antigens and an immunoblot using type-specific recombinant glycoproteins. equivocal results were reso ... | 2000 | 10797375 |
| monophosphoryl lipid a and qs21 increase cd8 t lymphocyte cytotoxicity to herpes simplex virus-2 infected cell proteins 4 and 27 through ifn-gamma and il-12 production. | we have shown previously that ifn-gamma pretreatment of human epidermal cells (ecs) cultured in vitro partially reverses down-regulation of surface mhc class i by hsv infection, allowing recognition by cd8 ctls, and that hsv immediate early (ie)/early (e) proteins are the predominant targets for cd8 ctls. in this study of 25 subjects, cd8 ctls recognized the hsv-2 ie infected cell protein 27 (icp27) (expressed in autologous ifn-gamma-pretreated, vaccinia virus recombinant-infected ecs) in all su ... | 2000 | 10799875 |
| herpes simplex virus type 2 growth and latency reactivation by cocultivation are inhibited with antisense oligonucleotides complementary to the translation initiation site of the large subunit of ribonucleotide reductase (rr1). | antisense oligonucleotides complementary to the translation initiation site of the herpes simplex virus type 2 (hsv-2) large subunit of ribonucleotide reductase (rr1) were studied for their ability to inhibit rr1 expression, hsv-2 growth, and its reactivation from latently infected ganglia. the oligomers caused a significant decrease (90%-97% inhibition) in hsv-2 rr1 expression and inhibited hsv-2 growth, with ic50 and ic90 values of 0.11 and 1.0 microm, respectively. the titers of hsv-2 mutants ... | 2000 | 10805158 |
| [the role of different microorganisms in the genesis of tubal-peritoneal infertility and in immunity]. | the character of microflora, directly causing inflammatory process in tuboperitoneal infertility, was studied. the study revealed the prevalence of microbial associations, chlamydia infection was detected in half of the women, and in 17% of the patients this infection was for the first time detected in the upper section of their reproductive apparatus, which was indicative of the role of chlamydia infection in the occlusion of the uterine tubes. opportunistic microflora persisting in persons wit ... | 2000 | 10808586 |
| decreased vaginal disease in j-chain-deficient mice following herpes simplex type 2 genital infection. | j-chain-deficient (jch(-/-)) mice were used to study the role of polymeric iga (piga) in primary disease and protective immunity following genital herpes simplex type 2 (hsv-2) infection. vaginal iga in the jch(-/-) mice was composed primarily of monomeric iga and was not associated with secretory component (sc). in contrast, vaginal iga in wild-type (wt) mice was predominantly polymeric and bound to sc. following hsv-2 genital infection, the jch(-/-) mice consistently exhibited fewer vaginal sy ... | 2000 | 10814580 |
| virological diagnosis of herpes simplex encephalitis. | the herpes simplex encephalitis (hse) represents one of the most severe infectious diseases of the central nervous system. as effective antiviral drugs are available, rapid and reliable diagnosis has become important. | 2000 | 10814936 |
| hsv-2 specific serology should be offered routinely to antenatal patients. | the most devastating consequence of genital herpes is neonatal herpes. it is clear that the majority of newborns acquire their infection by contact with infected genital secretions during delivery from an asymptomatic mother who acquired a first episode of genital herpes near the time of labour. since the majority of cases of first episode genital herpes during pregnancy are unrecognised, the prevention of neonatal transmission will depend upon the identification of the hsv serologically discord ... | 2000 | 10815025 |
| hsv-2 specific serology should not be offered routinely to antenatal patients. | the proposal to introduce antenatal screening for hsv has no evidence for a public or individual health benefit; indeed, it has the potential to increase anxiety for patients, with a minimal likelihood of reducing the risk of neonatal herpes infection. antenatal screening of an essentially healthy population of women must be validated in settings of different rates of neonatal hsv infection and the purposes and limitations of screening clearly outlined. identification of pregnant women at risk o ... | 2000 | 10815026 |
| herpes simplex virus peritonitis. | 2000 | 10816164 | |
| difference in incidence of spontaneous mutations between herpes simplex virus types 1 and 2. | spontaneous mutations within the herpes simplex virus (hsv) genome are introduced by errors during dna replication. indicative of the inherent mutation rate of hsv dna replication, heterogeneous hsv populations containing both acyclovir (acv)-resistant and acv-sensitive viruses occur naturally in both clinical isolates and laboratory stocks. wild-type, laboratory-adapted hsv type 1 (hsv-1) strains kos and cl101 reportedly accumulate spontaneous acv-resistant mutations at a frequency of approxima ... | 2000 | 10817703 |
| premarket evaluation of the pockit hsv-2 type-specific serologic test in culture-documented cases of genital herpes simplex virus type 2 [see comment]. | the genital herpes epidemic continues, in part, because patients with subclinical or atypical presentations cannot be identified by most herpes simplex virus (hsv) antibody tests. a new product, pockit hsv-2, has been developed to rapidly and accurately detect antibodies to hsv type 2 (hsv-2) in capillary blood or serum. | 2000 | 10821598 |
| testing for herpes simplex virus type 2: full-steam ahead? | 2000 | 10821599 | |
| seroprevalence of herpes simplex virus type 2 infection among attendees of a sexually transmitted disease clinic in italy. italian herpes forum. | an increased prevalence of herpes simplex virus type 2 (hsv-2) infection has been recently observed in industrialized countries. | 2000 | 10821604 |
| monoclonal antibodies suitable for type-specific identification of herpes simplex viruses by a rapid culture assay. | eight monoclonal antibodies (mabs) to herpes simplex viruses 1 and/or 2 (hsv-1, hsv-2) were tested for their reactivity with 190 previously typed hsv-positive clinical specimens in order to determine their suitability for use as type-specific reagents. using a rapid culture assay we found that two mabs (t51 and t96) identified hsv-1 in all the 94 specimens, previously found hsv-1-positive, but did not react with the 96 specimens, previously found hsv-2-positive. in contrast, one mab (303) confir ... | 1999 | 10825932 |
| a novel human gene similar to the protein kinase (pk) coding domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) codes for a serine-threonine pk and is expressed in melanoma cells. | the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is a multifunctional protein that contains a serine-threonine protein kinase (pk) activity (nelson, j. w., zhu, j. , smith, c. c., kulka, m., and aurelian, l. (1996) j. biol. chem. 271, 17021-17027). phylogenetic analyses indicated that icp10 pk belongs to a distinct subfamily of growth factor receptor serine-threonine pks that are characterized by their ability to function with a limited number of conserved cataly ... | 2000 | 10833516 |
| a homogenous 384-well high throughput screen for novel tumor necrosis factor receptor: ligand interactions using time resolved energy transfer. | the herpes virus entry mediator (hvem) receptor and its ligand, hvem-l, are involved in both herpes simplex virus type-1 (hsv-1) herpes simplex virus type-2 (hsv-2) infection, and in t-cell activation such that antagonists of this interaction are expected to have utility in viral and inflammatory diseases. in this report we describe the configuration of a homogeneous 384-well assay based on time-resolved energy transfer from a europium chelate on the hvem receptor to an allophycocyanin (apc) acc ... | 1999 | 10838440 |
| synthesis and biological activities of new carbaacyclonucleosides and 1'-oxaacyclonucleosides related to clitocine. | we describe the synthesis of two series of acyclonucleosides:carbaacyclonucleosides and 1'-oxaacyclonucleosides which possess the same aglycone as clitocine 3 which is a natural nucleoside exhibiting interesting biological properties. these compounds have been obtained by condensation of 4-aminobutanol or 3-silyloxypropoxyamine with 4,6-dichloro-5-nitropyrimidine. structural modifications have been made on the heterocyclic base and the side chain to enhance their potential activity. all these co ... | 2000 | 10843493 |
| herpes simplex type 2 infections--an update. | 2000 | 10844903 | |
| [monoclonal antibodies in the diagnosis of infections caused by the herpes simplex virus]. | a series of hybridomas producing monoclonal antibodies (mcab), specifically interacting with herpes simplex virus (hsv) proteins, types 1 and 2, has been obtained. mcab 7c4 and 4f6 have been shown to be highly active in the solid-phase enzyme immunoassay (eia) and to produce no reaction with hsv antigen in the indirect immunofluorescent assay (iifa). mcab 2b6, 3e5, 4a, 2c effectively detect mcab in iifa, but not eia, while mcab 3d 10 exhibit activity in both biochemical assays. moreover, as esta ... | 1999 | 10852033 |
| the antiviral activity exerted by vaccinia virus on the growth of herpes simplex virus in bs-c-1 cells. | the growth of herpes simplex virus type 2 (hsv-2) in bs-c-1 cells, was inhibited following super-infection with vaccinia virus. this inhibition was efficiently induced by both the intracellular mature virus (imv) form of vaccinia virus and the extracellular enveloped virus (eev), containing an additional external viral membrane. treatment of vaccinia imv with the detergents np-40, brij-58 or n-octyl-alpha-d-glucopyranoside, abolished its ability to inhibit the growth of hsv-2. ultraviolet irradi ... | 2000 | 10854666 |
| herpes simplex virus: the importance of asymptomatic shedding. | herpes simplex virus (hsv) is frequently shed after infection of the genital or perianal area. hsv shedding, as determined by culture, occurs on about 3% of days for immunocompetent women and men, and more for persons with hiv infection or if measured by polymerase chain reaction (pcr). most horizontal and vertical transmission of hsv occurs during unrecognized or asymptomatic shedding, and the majority of hsv-2-infected persons are unaware of their infection. many persons with 'asymptomatic' hs ... | 2000 | 10855766 |
| testing for type-specific antibody to herpes simplex virus--implications for clinical practice. | recently, assays that can distinguish between antibody to herpes simplex virus (hsv) types 1 and 2 have become available. these tests not only make it possible to better define infection in symptomatic patients and their sexual partners but also to identify asymptomatic infected individuals who may, nevertheless, be infectious. type-specific antibody tests for hsv have several potential applications. they have a clear role in helping to define the worldwide distribution and pattern of hsv infect ... | 2000 | 10855767 |
| peptide based enzyme-linked immunoassays for detection of anti-hsv-2 igg in human sera. | glycoprotein g of hsv-2 (gg2) and a peptide, corresponding to a previously recognised immunodominant epitope spanning residues 561-578 of the protein, were compared directly for type-specific serodiagnosis of hsv-2. the protein was affinity purified and obtained in a commercially available eia kit while the peptide, previously designated as peptide 55, was made as a multiple antigenic peptide. a panel of 100 characterised serum samples (60 hsv-2 positive, 20 hsv-1 positive and 20 hsv negative) w ... | 2000 | 10856753 |
| a monoclonal blocking eia for herpes simplex virus type 2 antibody: validation for seroepidemiological studies in africa. | a competitive type-specific enzyme-linked immunosorbent assay (elisa) for herpes simplex virus type 2 (hsv-2) antibody was developed using an infected cell antigen and a monoclonal antibody to glycoprotein g-2. this assay has been validated for use for epidemiological studies using a large panel of sera collected in rural uganda and a panel of 143 sera characterised previously by western blotting, the 'gold standard' for hsv type-specific serology. this evaluation was found to have a sensitivity ... | 2000 | 10856754 |
| detection of human serum antibodies against type-specifically reactive peptides from the n-terminus of glycoprotein b of herpes simplex virus type 1 and type 2 by surface plasmon resonance. | a single-step surface plasmon resonance protocol for the detection of antibodies against herpes simplex virus type 1 and type 2 (hsv-1, hsv-2) in human sera was established using the biacore system. two peptides from corresponding segments of the n-terminus of hsv-1 and hsv-2 glycoprotein b (gb), i.e. peptide gb-1 (60-73) (gaaptgdpkpkknk) and peptide gb-2 (55-68) (spattkarkrktkk), were identified as immunogenic. employing both peptides as diagnostic antigens in the surface plasmon resonance assa ... | 2000 | 10856760 |
| clinical management of selected oral fungal and viral infections during hiv-disease. | the purpose of this review is to describe current possibilities of management of selected fungal and viral oral opportunistic infections including oral candidiasis, herpes simplex type 1 and 2-related lesions (hsv1,2), oral hairy leukoplakia (ohl) and oral lesions associated with human papilloma viruses (hpv). less common diseases such as cytomegalovirus infection or human herpes virus type 8 associated with kaposi's sarcoma and others are not considered. in a number of instances lifelong therap ... | 1999 | 10858761 |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| role of serology in the diagnosis of herpes simplex encephalitis. | twenty one cerebrospinal fluid (csf)/brain tissue (16 csf and 5 brain tissue) from patients clinically suspected of herpes simplex encephalitis (hse) were collected during one year period and was subjected for the detection of hsv type i and type ii antigen by direct immunofluorescence (dif). paired serum and csf samples obtained from 12 patients were tested for herpes simplex virus antibodies by indirect immunofluorescence test. of the 21 cases, two were positive for hsv-1 antigen in csf and on ... | 1999 | 10862294 |
| disinfection of eyelid specula with chlorhexidine gluconate (hibiclens) after examinations for retinopathy of prematurity. | the preferred method of cleaning eyelid specula between examinations for retinopathy of prematurity is unknown. a previous study showed that disinfection with 70% isopropyl alcohol swabs fails to eliminate viruses and bacteria from the specula. | 2000 | 10865315 |
| maternal intrauterine herpes simplex virus infection leading to persistent fetal vasculature. | herpes simplex virus can cause serious ocular and systemic disease in the neonate. the mode of transmission to the neonate is usually from the maternal birth canal to the fetus intrapartum; but much more rarely, hematogenous transplacental infection can affect the developing fetus months prior to birth. persistent fetal vasculature occurs when there is persistence of the fetal ocular vasculature, which normally regresses prior to birth. to our knowledge, we report the first case of serologically ... | 2000 | 10865323 |
| [studies on the antiviral activity of silver sulfathiazole]. | silver sulfathiazole shows strong antibacterial activity and good tolerance after topical application. the aim of the study was to determine the antiviral activity of silver sulfathiazole in tissue culture after incubation of drug and virus. the antiviral activity was measured after various periods of exposure and at different drug concentrations. the results obtained indicate the activity of silver sulfathiazole against herpesvirus type 1 and type 2. this drug suppresses or completely inactivat ... | 1999 | 10865442 |
| superiority of intramuscular route and full length glycoprotein d for dna vaccination against herpes simplex 2. enhancement of protection by the co-delivery of the gm-csf gene. | immunization with naked dna has been analyzed in two critical variables: the site of injection and the cellular compartment to which the coded protein is directed. the gene for the full length of the glycoprotein d (gd) of hsv-2 under the control of the citomegalovirus (cmv) promoter was injected via the intradermal (i.d.) or the intramuscular (i.m.) routes in mice. immunization in the quadricep muscle was superior to the intradermal immunization in the footpads. a stronger activation of ifn-gam ... | 2000 | 10869769 |
| molecular evidences for a role of hsv-1 in multiple sclerosis clinical acute attack. | to verify the possible role of human herpesviruses as triggering or aggravating factors in relapsing-remitting multiple sclerosis (rrms) clinical acute attack, we studied the prevalence of some herpesviruses in the peripheral blood mononuclear cells (pbmcs) collected from 22 ms patients during an ms relapse and in a stable phase and from 18 healthy controls (hc). dna belonging to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hu ... | 2000 | 10871797 |
| serological prevalence of herpes simplex virus type 2 amongst gum clinic attenders in a district general hospital setting. | our objective was to determine the seroprevalence of herpes simplex virus (hsv) type 2 infection amongst genitourinary medicine (gum) clinic attenders at a district general hospital using a commercially available enzyme immunoassay (eia). in a prospective study, heterosexual patients attending the department of gum at trafford general hospital attending with a new clinical problem and having a blood sample taken for routine syphilis serology had the same sample tested for hsv type 2 antibodies. ... | 2000 | 10872911 |
| [the detection of the herpes simplex virus 1 and 2 antigens in clinical specimens by using monoclonal antibodies]. | the possibility of using monoclonal antibodies (mcab), obtained earlier, for the detection of herpes simplex virus (hsv) in clinical specimens taken from sick and infected persons was studied. the examination of 90 persons revealed that the mixture of mcab 4a and 2c could effectively detect the presence of hsv antigen in the indirect immunofluorescence assay (ifa) directly in cells contained in cytological preparations (smears, scrapes, impressions) obtained from different organs of patients. th ... | 1999 | 10876858 |
| [a killed commercial vaccine against herpes simplex viruses 1 and 2 as an immunocorrective agent in chronic herpetic infection]. | 1999 | 10876868 | |
| herpes simplex virus-type 2 seropositivity in a danish adult population denying previous episodes of genital herpes. | 2000 | 10877149 |