Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| disabled infectious single cycle (disc) herpes simplex virus as a vector for immunotherapy of cancer. | 1998 | 10026900 | |
| effect of undecylenic acid as a topical microbicide against genital herpes infection in mice and guinea pigs. | there is increasing interest in the use of topical microbicides to help prevent the spread of sexually transmitted diseases (std). undecylenic acid (ua), a monosaturated fatty acid, is the active ingredient in a number of over-the-counter (otc) antifungal spray powders, that also exhibits in vitro antibacterial and antiviral activity, including herpes simplex virus (hsv) activity. we, therefore, evaluated ua as a topical microbicide against genital hsv infection using the murine and guinea pig m ... | 1999 | 10027648 |
| development of a competitive elisa for detection of primates infected with monkey b virus (herpesvirus simiae). | two competitive elisas (c-elisas) are described that allow detection of antibodies against monkey b virus (bv, cercopithecine herpesvirus 1). the assays utilize monoclonal antibodies (mabs) directed against the bv glycoprotein b (gb). two of these mabs specifically recognize bv gb while a third mab also reacts with the gb homologues of other primate alpha-herpesviruses (herpes simplexvirus-1, hsv-1: hsv-2; simian agent-8, sa8; and herpesvirus papio-2, hvp2). a c-elisa using the single cross-reac ... | 1999 | 10029325 |
| herpes in the time of aids: a comparison of the epidemiology of hiv-1 and hsv-2 in young men in northern thailand. | to compare the epidemiologic pattern of hiv-1, a recently introduced sexually transmitted disease (std) agent in thailand, with the pattern of hsv-2, a well-established std agent, so that future trends for both viruses can be better understood. | 1999 | 10029978 |
| improving the management of genital herpes. | most patients with herpes simplex virus infection have mild or subclinical disease and fail to seek medical attention or are misdiagnosed. but inexpensive and accurate new serologies are being developed. chronic suppressive therapy in patients with frequent recurrences reduces viral shedding and may reduce transmission. | 1999 | 10047759 |
| synthesis of 2'-methylene-substituted 5-azapyrimidine, 6-azapyrimidine, and 3-deazaguanine nucleoside analogues as potential antitumor/antiviral agents. | 2'-deoxy-2'-methylene-6-azauridine (5) and 2'-deoxy-2'-methylene-6-azacytidine (8) have been synthesized via a multi-step procedure from 6-azauridine. 2'-deoxy-2'-methylene-5-azacytidine (14a) and 2'-deoxy-2'-methylene-3-deazaguanosine (19a) and their corresponding alpha-anomers (14b and 19b) have been synthesized by the transglycosylation of 3',5'-o-(1,1,3,3- tetraisopropyldisiloxane-1,3-diyl)-2'-deoxy-2'-methyleneu ridine (12) with silylated 5-azacytosine and silylated n2-palmitoyl-3-deazaguan ... | 1999 | 10048223 |
| seroprevalence study of herpes simplex virus type 2 among pregnant women in germany using a type-specific enzyme immunoassay. | in a german seroepidemiological study to determine the proportion of pregnant women infected with herpes simplex virus type 2 (hsv-2) and at risk of transmitting the infection to the newborn during delivery, igg antibodies to hsv-2 in 1999 sera collected from pregnant women in 1996-1997 were measured using an automated type-specific enzyme immunoassay (cobas core hsv-2 igg eia; roche diagnostics, switzerland). the seroprevalence of hsv-2 was 8.9%, and control studies with a type-common hsv assay ... | 1998 | 10052553 |
| il-12 gene as a dna vaccine adjuvant in a herpes mouse model: il-12 enhances th1-type cd4+ t cell-mediated protective immunity against herpes simplex virus-2 challenge. | il-12 has been shown to enhance cellular immunity in vitro and in vivo. recent reports have suggested that combining dna vaccine approach with immune stimulatory molecules delivered as genes may significantly enhance ag-specific immune responses in vivo. in particular, il-12 molecules could constitute an important addition to a herpes vaccine by amplifying specific immune responses. here we investigate the utility of il-12 cdna as an adjuvant for a herpes simplex virus-2 (hsv-2) dna vaccine in a ... | 1999 | 10072541 |
| prevalence of herpes simplex virus types 1 and 2, cytomegalovirus, and varicella-zoster virus infections in eritrea. | herpesviruses establish latent infections in their hosts for life. the scarcity of data that exists in regard to herpesvirus infections in many african regions, could partly be due to the mild nature of their primary infections and the lack of means for their proper diagnosis. however, in recent decades the alarming spread of hiv infection in africa and associated frequent reactivation of herpesvirus infections is leaving less room for neglect. this seroprevalence study is intended to help in th ... | 1999 | 10073414 |
| evaluation of elvis hsv id/typing system for the detection and typing of herpes simplex virus from clinical specimens. | herpes simplex virus (hsv) is a common pathogen with two serotypes: hsv-1 and hsv-2. hsv infection does not pose much of a threat to an immunocompetent host but to an immunocompromised host or a neonate the infection can be fatal. the enzyme-linked virus inducible system (elvis) employs a genetically altered baby hamster kidney (bhk) cell line that allows for the rapid overnight detection of hsv but also includes an immunofluorescent stain for the simultaneous detection and typing of hsv-1 and h ... | 1999 | 10073415 |
| amplification of the six major human herpesviruses from cerebrospinal fluid by a single pcr. | we used a novel type of primer system, a system that uses stair primers, in which the primer sequences are based on consensus sequences in the dna polymerase gene of herpesvirus to detect herpesviruses by pcr. a single pcr in a single tube detected the six major herpesviruses that infect the central nervous system: herpes simplex virus type 1 (hsv-1), and type 2 (hsv-2), cytomegalovirus (cmv), epstein-barr virus (ebv), varicella-zoster virus (vzv), and human herpesvirus 6 (hhv-6). we used the te ... | 1999 | 10074507 |
| interleukin-4-mediated inhibition of nitric oxide production in interferon-gamma-treated and virus-infected macrophages. | upon interferon-gamma (ifn-gamma) stimulation, murine macrophages (mphi) produce nitric oxide (no) through expression of inducible nitric oxide synthase (inos). interleukin (il)-4 treatment, even delayed 12 h relative to ifn-gamma, antagonized this induction, whereas infection with herpes simplex virus type 2 (hsv-2) or treatment with tumour necrosis factor-alpha exerted a synergistic effect, which partly compensated for the antagonistic effect of il-4. neither il-4 nor hsv-2 affected the ifn-ga ... | 1999 | 10075021 |
| [virology, epidemiology and diagnosis of herpes genitalis]. | 1999 | 10081481 | |
| directed evolution of thymidine kinase for azt phosphorylation using dna family shuffling. | the thymidine kinase (tk) genes from herpes simplex virus (hsv) types 1 and 2 were recombined in vitro with a technique called dna family shuffling. a high-throughput robotic screen identified chimeras with an enhanced ability to phosphorylate zidovudine (azt). improved clones were combined, reshuffled, and screened on increasingly lower concentrations of azt. after four rounds of shuffling and screening, two clones were isolated that sensitize escherichia coli to 32-fold less azt compared with ... | 1999 | 10096293 |
| the immunosuppressive agent mycophenolate mofetil markedly potentiates the activity of lobucavir [1r(1alpha,2beta,3alpha)]-9-[2,3-bis(hydroxymethyl)cyclobutyl]guanine against different herpes viruses. | mycophenolate mofetil (mmf) has been approved as an immunosuppressive agent in kidney transplant recipients and may thus be used concomitantly with antiherpetic agents, the latter for the treatment of intercurrent herpesvirus infections. the parent compound of mmf, mycophenolic acid (mpa), is a potent inhibitor of inosine monophosphate dehydrogenase and causes depletion of the intracellular (deoxy)guanosine triphosphate [(d)gtp] pools. lobucavir [1r(1alpha,2beta,3alpha)]-9-[2,3-bis(hydroxymethyl ... | 1999 | 10096537 |
| the primary production of an infectious recombinant herpes simplex virus vaccine. | the production and extracellular release of a recombinant herpes simplex virus (type 2) from monolayers of infected complementing vero cells (cr2) are addressed. growth and virus production conditions are identified that provide adequate virus titers with cell seeding densities and viral multiplicities of infection that could be reasonably handled in manufacturing. harvesting by sonication of cell monolayers is shown to give the highest recovery of infectious virus (to 2.5 x 10(6) pfu/ml) but le ... | 1998 | 10099202 |
| the affinity adsorptive recovery of an infectious herpes simplex virus vaccine. | the chromatographic purification of a recombinant herpes simplex virus (type 2) from salt- and heparin-released harvests of infected complementing vero (cr2) cells is addressed. functionalized matrices and process operating conditions are identified that provide adequate virus titres in eluates that are significantly reduced in cr2 cell protein and dna and possess a low level of hsv-2 protein. virus from diluted salt-released harvests (0.14 m nacl) was not appreciably adsorbed onto either hepari ... | 1999 | 10099562 |
| herpes simplex virus entry is associated with tyrosine phosphorylation of cellular proteins. | the initial step in herpes simplex virus (hsv) entry is binding of virion glycoprotein (g)c and/or gb to cell surface heparan sulfate. after this initial attachment, gd interacts with cell surface receptor or receptors, and the virion envelope fuses with the cell membrane. fusion requires viral glycoproteins gb, gd, gl, and gh, but the cellular factors that participate in or the pathways activated by viral entry have not been defined. to determine whether signal transduction pathways are trigger ... | 1999 | 10191187 |
| association of major histocompatibility complex determinants with the development of symptomatic and asymptomatic genital herpes simplex virus type 2 infections. | the clinical spectrum of herpes simplex virus (hsv) infections, ranging from asymptomatic to frequently distressing outbreaks, suggests that there may be immunologic determinants of disease severity that are associated with human leukocyte antigen (hla) expression. a controlled, prospective study identified several major histocompatibility complex (mhc) class i and ii antigens whose frequencies are associated with hsv-2 infection or with frequent symptomatic genital recurrences. previous studies ... | 1999 | 10191207 |
| antiviral activities of biflavonoids. | biflavonoids such as amentoflavone (1), agathisflavone (2), robustaflavone (3), hinokiflavone (4), volkensiflavone (5), rhusflavanone (7), succedaneflavanone (9), all isolated from rhus succedanea and garcinia multiflora, as well as their methyl ethers and acetates, volkensiflavone hexamethyl ether (6), rhusflavanone hexaacetate (8), and succedaneflavanone hexaacetate (10) were evaluated for their antiviral activities. the inhibitory activities against a number of viruses including respiratory v ... | 1999 | 10193201 |
| public and personal health implications of asymptomatic viral shedding in genital herpes. | 1998 | 10195042 | |
| control of stds--the role of prophylactic vaccines against herpes simplex virus. | to summarise the current status of genital herpes simplex virus (hsv) vaccine development and provide a discussion of the potential benefits and limitations of genital herpes vaccines. | 1998 | 10195045 |
| cellular elongation factor 1delta is modified in cells infected with representative alpha-, beta-, or gammaherpesviruses. | earlier reports (y. kawaguchi, r. bruni, and b. roizman, j. virol. 71:1019-1024, 1997; y. kawaguchi, c. van sant, and b. roizman, j. virol. 72:1731-1736, 1998) showed that herpes simplex virus 1 (hsv-1) infection causes the hyperphosphorylation of translation elongation factor 1delta (ef-1delta) and that the modification of ef-1delta is the consequence of direct phosphorylation by a viral protein kinase encoded by the ul13 gene of hsv-1. the ul13 gene is conserved in members of all herpesvirus s ... | 1999 | 10196346 |
| the murine homolog (mph) of human herpesvirus entry protein b (hveb) mediates entry of pseudorabies virus but not herpes simplex virus types 1 and 2. | a mouse member of the immunoglobulin superfamily, originally designated the murine poliovirus receptor homolog (mph), was found to be a receptor for the porcine alphaherpesvirus pseudorabies virus (prv). this mouse protein, designated here murine herpesvirus entry protein b (mhveb), is most similar to one of three related human alphaherpesvirus receptors, the one designated hveb and also known as poliovirus receptor-related protein 2. hamster cells resistant to prv entry became susceptible upon ... | 1999 | 10196354 |
| genital herpes simplex virus type 2 shedding is increased in hiv-infected women in africa. | 1999 | 10197391 | |
| [mollaret's meningitis]. | 1999 | 10201123 | |
| [radiculomyelopathy due to genital herpes simplex virus infection (elsberg syndrome)]. | 1999 | 10201134 | |
| [herpes simplex virus hepatitis]. | 1999 | 10201154 | |
| cofactors for the acquisition of hiv-1 among heterosexual men: prospective cohort study of trucking company workers in kenya. | to determine the prevalence, incidence, and correlates of hiv-1 infection in a cohort of east african trucking company employees. | 1999 | 10203386 |
| evaluation of three glycoprotein g2-based enzyme immunoassays for detection of antibodies to herpes simplex virus type 2 in human sera. | three new glycoprotein g-based enzyme immunoassays (eti-hsvk-g 2, sorin diagnostics biomedica [assay a]; hsv type 2 specific igg elisa, gull laboratories, inc. [assay b]; cobas core hsv-2 igg eia, roche [assay c]) for the detection of herpes simplex virus (hsv) type 2 (hsv-2)-specific antibodies were evaluated. by testing sera from 25 individuals with culture-proven hsv-2 infection, the assays showed a sensitivity of 96%. the specificities, evaluated with sera from 70 hsv antibody-negative child ... | 1999 | 10203464 |
| laboratory diagnosis of common viral infections of the central nervous system by using a single multiplex pcr screening assay. | a multiplex pcr assay that detects the four commonest causes of viral meningitis and encephalitis in the united kingdom (herpes simplex virus [hsv] type 1 [hsv-1], hsv type 2 [hsv-2], varicella-zoster virus [vzv], and enteroviruses) was developed, and its sensitivity was compared with those of similar assays described previously for this application. compared to the previous assays, this single multiplex pcr assay had higher molecular sensitivities for the detection for each of the viruses and i ... | 1999 | 10203485 |
| ability of a rapid serology test to detect seroconversion to herpes simplex virus type 2 glycoprotein g soon after infection. | sera (n = 188) from 29 patients with first-episode genital herpes simplex virus type 2 (hsv-2) infections were tested by pockit-hsv-2 and western blot (wb) to determine the speed of seroconversion. the median time to detection of hsv-2 antibody was 13 days (range, 3 to 102 days) by the pockit-hsv-2 test versus 13 days (range, 2 to 58 days) for wb. | 1999 | 10203544 |
| neonatal stridor in association with herpes simplex infection of the larynx. | herpes simplex virus (hsv) infection in the neonatal period may be confined to the eyes, skin and upper aerodigestive tract or may be widely disseminated to other organs, with particular recognition of involvement of the central nervous system (cns) causing herpes encephalitis (whitley et al., 1980a, b; andersen, 1987). primary laryngeal hsv infection is extremely uncommon. we present a case of acute neonatal stridor secondary to such localized disease and discuss its management. | 1998 | 10209621 |
| epidemiology of genital herpes - recent advances. | genital herpes is a common, distressing infection which, due to increasing incidence world-wide, has become a prominent public health issue over recent years, even throughout the decade of human immunodeficiency virus (hiv). since the late 1970's, the prevalence of herpes simplex virus type 2 (hsv-2) infection has increased by approximately 30 percent in the us. the number of sufferers world-wide is currently estimated at approximately 86 million people. new serological methods based on the dete ... | 1999 | 10210781 |
| herpes simplex virus infection in pregnancy: diagnosis and significance. | herpes simplex viruses (hsv) cause genital infections, and hsv infection during pregnancy can result in infection of the neonate. here hsv structure and replication,viral pathogenesis, human immune responses to hsv, the epidemiology, details of hsv diagnosis with emphasis on typing, clinical features and antiviral treatment are reviewed. the importance of appropriate diagnostic procedures in relation to correct risk assessment of neonatal infection is emphasized. | 1998 | 10213894 |
| detection of herpes simplex virus in genital specimens by type-specific polymerase chain reaction. | viral isolation is the standard method for the detection of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in clinical specimens. this study describes the development of a type-specific polymerase chain reaction (pcr) assay for detection and typing of hsv-1 and hsv-2, and a comparison of its sensitivity with that of isolation in a clinical setting. specimens from patients presenting with genital ulcers were tested for the presence of hsv by both methods. oligonucleotide primers were sele ... | 1999 | 10215112 |
| risk factors for herpes simplex virus type 2 infection among female commercial sex workers in mexico city. | a scarce number of studies have been carried out to determine the epidemiology of herpes simplex virus type 2 (hsv-2) infection in female sex workers (fsws). the objective of this study was to examine the correlates of infection for hsv-2 with socioeconomic and demographic characteristics, sexual behaviour and history of sexually transmitted diseases (stds) among fsws in mexico city. a sample frame of commercial sex work sites was constructed during the fall of 1992. sites identified were street ... | 1999 | 10215115 |
| performance of a rapid screening test pockit hsv2 rapid test. | 1999 | 10215136 | |
| vaccine potential of a herpes simplex virus type 2 mutant deleted in the pk domain of the large subunit of ribonucleotide reductase (icp10). | a herpes simplex virus type 2 (hsv-2) mutant deleted in the pk domain of the large subunit of ribonucleotide reductase (icp10) was evaluated as a potential vaccine for the prevention of hsv-2 infection and disease. this virus, designated icp10 deltapk, expressed a 95 kda icp10 protein that lacked pk activity and transforming potential. icp10 deltapk was growth compromised in dividing and nondividing cells in culture. in dividing cells, onset of virus growth was delayed, with replication initiati ... | 1999 | 10217594 |
| effects of dna immunization formulated with bupivacaine in murine and guinea pig models of genital herpes simplex virus infection. | the immunogenicity and efficacy of a herpes simplex virus type 2 glycoprotein d (gd2) dna vaccine formulated with bupivacaine was evaluated using murine and guinea pig models of genital herpes. animals received three doses of 100 microg of gd2 plasmid or control plasmid intramuscularly prior to intravaginal challenge with hsv-2. immunization induced hsv elisa and neutralizing antibody in serum and elisa antibody in the vaginal secretions of all animals evaluated. following intravaginal hsv-2 cha ... | 1999 | 10217595 |
| anti-herpes simplex virus activity of alkaloids isolated from stephania cepharantha. | by screening water and meoh extracts of 30 chinese medicinal plants for their anti-herpes simplex virus (hsv)-1 activity, a meoh extract of the root tubers of stephania cepharantha hayata showed the most potent activity on the plaque reduction assay with an ic50 value of 18.0 microg/ml. of 49 alkaloids isolated from the meoh extract, 17 alkaloids were found to be active against hsv-1, including 13 bisbenzylisoquinoline, 1 protoberberine, 2 morphinane and 1 proaporphine alkaloids, while benzyliso ... | 1999 | 10220283 |
| you're the flight surgeon. herpes simplex virus type 2(hsv-2). | 1999 | 10223273 | |
| disseminated herpes simplex virus infection in a renal transplant patient as possible cause of repeated urinary extravasations. | disseminated herpes simplex virus type 2 (hsv-2) infections are infrequent in patients receiving organ transplants, but usually have a poor outcome. we describe the case of a renal transplant patient who developed a disseminated hsv-2 infection with repeated urinary extravasations. the diagnosis was carried out using a multiplex polymerase chain reaction nested assay and it suggested hsv-2 as a possible cause of repeated urinary fistulas. | 1999 | 10224485 |
| clinical course of patients with serologic evidence of recurrent genital herpes presenting with signs and symptoms of first episode disease. | the care of patients with first episode and recurrent genital herpes differs with respect to therapy and source partner evaluation. of 498 persons who presented with what appeared by history and symptoms to be a first episode of genital herpes, we identified 41 who had serologic evidence of remotely acquired herpes simplex virus 2 (hsv-2) infection. | 1999 | 10225590 |
| clinical misidentification of patients with first episode genital herpes: a note of caution. | 1999 | 10225591 | |
| prevention agenda for genital herpes. | few meeting participants envisioned a prevention and control program on the scale or scope of cdc's programs to prevent hiv infection, syphilis, gonorrhea, and chlamydial infection, but all agreed that the virtual absence of public health interventions to prevent genital herpes is no longer appropriate in light of evolving epidemiologic knowledge and other research advances. the ultimate scope of a national genital herpes prevention effort will depend in part on the results of the recommended re ... | 1999 | 10225592 |
| prevalence of herpes simplex type 2 antibodies and a clinical history of herpes in three different populations in campinas city, brazil. | to determine the seroprevalence of herpes simplex virus type 2 (hsv-2) antibodies and the relation between the history of clinical herpes and the presence of type-specific hsv-2 antibodies in three different populations from the city of campinas city, brazil. | 1998 | 10225987 |
| changes in prevalence of herpes simplex virus type 1 and 2 antibodies from 1973 to 1993 in the rural districts of japan. | using the gg-capture elisa, changes in the seroprevalence of hsv-1 and hsv-2 from 1973 to 1993 were studied for 614 sera collected from general adults living in rural japan. the hsv-1 seroprevalence for men and women decreased from 75.3 and 80.6% in 1973 to 54.4 and 59.6%, respectively, in 1993. the hsv-2 seroprevalence also decreased from 10.2 and 9.9% in 1973 to 1.8 and 1.2%, respectively, in 1993. although the decrease in hsv-2 prevalence seemed to be correlated with the general decrease of s ... | 1999 | 10229273 |
| a new antiviral and antimicrobial sesquiterpene from glyptopetalum sclerocarpum. | sclerocarpic acid, a new sesquiterpene with a novel carbon skeleton, was isolated from the stem bark of glyptopetalum sclerocarpum laws. (celastraceae). the compound exhibited antiviral activity against herpes simplex virus types 1 and 2, and antimicrobial activity against gram-positive bacteria and a fungus. | 1999 | 10232073 |
| a case of vulvitis circumscripta plasmacellularis positive for herpes simplex type ii antigen. | 1998 | 10233608 | |
| development of a high-throughput quantitative assay for detecting herpes simplex virus dna in clinical samples. | we have developed a high-throughput, semiautomated, quantitative fluorescence-based pcr assay to detect and type herpes simplex virus (hsv) dna in clinical samples. the detection assay, which uses primers to the type-common region of hsv glycoprotein b (gb), was linear from <10 to 10(8) copies of hsv dna/20 microl of sample. among duplicate samples in reproducibility runs, the assay showed less than 5% variability. we compared the fluorescence-based pcr assay with culture and gel-based liquid hy ... | 1999 | 10325351 |
| new developments in the epidemiology, natural history and management of genital herpes. | the prevalence of genital herpes is increasing in several populations worldwide. factors that may be contributing to this increase include greater numbers of sexual partners, the high frequency of asymptomatic infections, poor use of safe sexual practices, and possibly the decreased incidence of childhood oral herpes simplex virus infection. transmission occurs via skin-to-skin or mucous membrane contact during periods of viral shedding when lesions are present but may also occur when the patien ... | 1999 | 10333138 |
| seroprevalence of herpes simplex virus-type 2 in african-american college women. | this study examines the relationship between sexual behaviors and prevalence of herpes simplex virus-type 2 (hsv-2) among african-american college women. subjects (n = 138) were recruited randomly from a state university to participate in a study regarding sexual attitudes and behaviors and to have their blood drawn for type-specific hsv seroprevalence. sera were analyzed for 96 college women with a mean age of 21 years. of the 96 women, 29 (30%) were hsv-2 seropositive. the results of this stud ... | 1999 | 10333670 |
| synthesis and antiviral activity of 6-benzoyl-benzoxazolin-2-one and 6-benzoyl-benzothiazolin-2-one derivatives. | the synthesis and antiviral activity of an original series of 6-benzoyl-benzoxazolin-2-one and 6-benzoyl-benzothiazolin-2-one derivatives are described. several compounds were found to have a selective inhibitory activity against human cytomegalovirus (hcmv) and varicella-zoster virus (vzv) in vitro, being inactive against a variety of other dna and rna viruses. 6-(3-fluorobenzoyl)benzoxazolin-2-one, 6-(3-fluorobenzoyl)benzothiazolin-2-one, 6-(3-bromobenzoyl)benzothiazolin-2-one, 6-(3-iodobenzoy ... | 1999 | 10335403 |
| fatal herpetic hepatitis in adult following short corticotherapy: a case report. | herpes simplex virus hepatitis (hsv hepatitis) is an uncommon and severe complication of hsv type 1 and hsv type 2 infection. hsv hepatitis affects mostly immunocompromised patients. we report the case of a young man without any previous known immunodeficiency who developed fatal hsv hepatitis in the first 8 days of oral corticotherapy given for ulcerative colitis. a prompt diagnosis was possible because hsv was recovered from peripheral blood leukocytes. | 1999 | 10342519 |
| the epidemiology of neonatal herpes simplex virus infections in california from 1985 to 1995. | comprehensive hospital discharge data completed by the california office of statewide health planning and development was used to determine whether the proportion of infants </=6 weeks of age who were hospitalized with a diagnosis of herpes simplex virus (hsv) infection changed between 1985 and 1995. during 1985, 1990, and 1995, respectively, 11.7, 11.3, and 11.4 infants per 100,000 live births had a diagnosis of hsv (p=.98). the proportion of infants 1-42 days of age who were discharged from th ... | 1999 | 10353880 |
| the topical microbicide pro 2000 protects against genital herpes infection in a mouse model. | vaginal gel formulations containing the naphthalene sulfonate polymer pro 2000 are being developed as topical microbicides to protect against infection with sexually transmitted disease (std) pathogens. a mouse model was used to determine whether pro 2000 could protect against genital herpes in vivo. animals received a single intravaginal application of 15 microl of a 10% pro 2000 aqueous solution or a 4.0% or 0.5% pro 2000 vaginal gel formulation 20 s prior to intravaginal challenge with 4.0 lo ... | 1999 | 10353881 |
| prevalence of serum antibodies to la-1 oncoprotein, herpes simplex virus type-2 glycoprotein and human papillomavirus type 16 transactivator (e2) protein among indian women with cervical neoplasia. | prevalence of serum antibodies to synthetic peptide to oncoprotein of la-1 known as oncogene of herpes simplex virus type-2, herpes simplex virus type-2 glycoprotein-d as an determinant of viral pathogenicity and human papillomavirus type 16 transactivator e2 protein was studied among 46 indian women with cervical neoplasia using immunoblot assay for hsv-2 gd glycoprotein and la-1 antibodies as well as peptide elisa assay to detect hpv16 e2 antibodies. the seropositivity to la-1 oncoprotein was ... | 1998 | 10356958 |
| herpes simplex virus infections in adolescents. | the intensity of herpes simplex virus in adolescents can range from trivial to life-threatening and, because therapy should begin early, a high index of suspicion is required. various forms of hsv-1 and hsv-2 infections addressed here includes gingivostomatitis, anogenital infection, herpetic whitlow, eye infections, encephalitis, and infection in the immunocompromised host. | 1995 | 10358302 |
| antibody responses to the herpes simplex virus type 2 glycoprotein g in sera of human immunodeficiency virus-infected patients in slovakia. | thirty sera of human immunodeficiency virus-positive (hiv+) and 37 sera of hiv-negative (hiv-) individuals in slovakia were tested for the presence of antibodies to herpes simplex virus type 2 (hsv-2) glycoprotein g (gg). a notable difference between the prevalence of hsv-2-specific antibodies in hiv+ and that in hiv- individuals was found (37% vs. 11%) confirming and extending previous reports that hsv-2 infection is an important risk factor for hiv transmission. efforts toward the detection of ... | 1998 | 10358733 |
| analysis of nucleotide sequence variations in herpes simplex virus types 1 and 2, and varicella-zoster virus. | to analyze the difference in the degree of divergence between genes from identical herpesvirus species, we examined the nucleotide sequence of genes from the herpes simplex virus type 1 (hsv-1) strains vr-3 and 17 encoding thymidine kinase (tk), deoxyribonuclease (dnase), protein kinase (pk; ul13) and virion-associated host shutoff (vhs) protein (ul41). the frequency of nucleotide substitutions per 1 kb in tk gene was 2.5 to 4.3 times higher than those in the other three genes. to prove that the ... | 1998 | 10358747 |
| novel triazole 2'-deoxy-4'-thionucleosides: stereoselective synthesis and biological evaluation. | a study on the use of protecting groups led to the employment of the para-methoxybenzoyl (pmb) group as a directing group in the synthesis of novel triazole 2'-deoxy-4'-thionucleosides. use of the pmb group gave alpha:beta ratios of 1:6 in the glycosylation step with azidotrimethylsilane. a series of novel triazoles were generated for in vitro antiviral evaluation. | 1999 | 10358943 |
| immunization against genital herpes with a vaccine virus that has defects in productive and latent infection. | an effective vaccine for genital herpes has been difficult to achieve because of the limited efficacy of subunit vaccines and the safety concerns about live viruses. as an alternative approach, mutant herpes simplex virus strains that are replication-defective can induce protective immunity. to increase the level of safety and to prove that replication was not needed for immunization, we constructed a mutant herpes simplex virus 2 strain containing two deletion mutations, each of which eliminate ... | 1999 | 10359827 |
| sequential peripartum herpes simplex virus type 2 disease in parents and their newborn mimicking intrafamily spread of common viruses. | herpes simplex type 2 (hsv2) disease developed sequentially among two parents and their newborn. the father first became ill with upper-respiratory symptoms and fever. then, 5 days later, shortly after delivery, the mother had fever, pharyngitis, and diarrhea. subsequently, the infant developed undifferentiated febrile illness at the age of 3 days. hsv etiology was recognized by incidental isolation of hsv2 from the newborn naospharynx. the father never developed genital lesions and the mother's ... | 1999 | 10362081 |
| the role of gamma interferon in immune resistance to vaginal infection by herpes simplex virus type 2 in mice. | we investigated the role of interferon gamma (ifn-gamma) in a mouse model of immunity to vaginal infection by herpes simplex virus type 2 (hsv-2). within 8 h after immune mice were challenged intravaginally with hsv-2, ifn-gamma concentrations in vaginal secretions reached levels that can be antiviral in vitro. this rapid synthesis of ifn-gamma occurred in immune-challenged mice but not in nonimmune-challenged mice, indicating that it required memory t cells. immunostaining and in situ hybridiza ... | 1999 | 10366565 |
| fulminant herpes hepatitis in a healthy adult: a treatable disorder? | hepatitis due to herpes simplex virus (hsv) is a potentially fatal disorder that is often not considered in the differential diagnosis of acute hepatitis. this disease occurs most often in patients with impaired immunity and is very uncommon in healthy patients. hsv hepatitis presents with a wide clinical spectrum, and the clinical diagnosis is difficult. we describe a case of disseminated herpes virus infection with fulminant hepatitis mimicking an acute human immunodeficiency virus infection i ... | 1999 | 10372946 |
| antiviral activity of sandalwood oil against herpes simplex viruses-1 and -2. | sandalwood oil, the essential oil of santalum album l., was tested for in vitro antiviral activity against herpes simplex viruses-1 and -2. it was found that the replication of these viruses was inhibited in the presence of the oil. this effect was dose-dependent and more pronounced against hsv-1. a slight diminution of the effect was observed at higher multiplicity of infections. the oil was not virucidal and showed no cytotoxicity at the concentrations tested. | 1999 | 10374251 |
| evaluation of a fully automated glycoprotein g-2 based assay for the detection of hsv-2 specific igg antibodies in serum and plasma. | ranking after infections with chlamydia trachomatis and human papillomavirus, genital herpesvirus is the third most common sexually transmitted disease. the majority of recurrent genital herpes infections are caused by herpes simplex virus type-2 (hsv-2). seroepidemiological studies on the prevalence of hsv-2 specific igg antibodies are especially important to study the impact of this infection among risk groups. | 1999 | 10382816 |
| the use of peptides from glycoproteins g-2 and d-1 for detecting herpes simplex virus type 2 and type-common antibodies. | identification and discrimination of latent herpes simplex virus (hsv) infection relies on antibody identification. the inclusion of synthetic peptides with hsv glycoproteins provides means for stable and discriminatory assays for population studies. | 1999 | 10382821 |
| herpetic salpingitis and fallopian tube prolapse. | we describe the unusual association of fallopian tubal prolapse and herpetic infection, an occurrence not previously reported to our knowledge. | 1999 | 10383700 |
| evaluation of anti-herpesvirus activity of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl]- guanine (a-5021) in mice. | the anti-herpesvirus activity of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl]guani ne (a-5021) was evaluated in murine cells and in several murine models of herpes simplex virus (hsv) infection. against hsv type 1 (hsv-1), a-5021 was 15-30- and 30-60-fold more active, and against hsv type 2 (hsv-2), it was 2- and 8-fold more active than acyclovir and penciclovir in balb/3t3 cells, respectively. when antiviral compounds were administered orally (once daily) to mice infected intra ... | 1999 | 10389656 |
| clinical reactivation of genital herpes simplex virus infection decreases in frequency over time. | visits to physicians for genital herpes simplex virus (hsv) infection continue to increase. most patients with symptomatic infections have recurrences, but no studies of the long-term clinical course of genital herpes are available. | 1999 | 10391810 |
| herpes simplex hepatitis in pregnancy: a case report and review of the literature. | fulminant hepatic dysfunction in the third trimester of pregnancy accompanied by fever may result from disseminated herpes simplex virus. since 1969, 24 cases of herpes simplex hepatitis, including the current case, have been reported. mucocutaneous lesions are present in only half of cases; therefore, suspicion for diagnosis of this disease is low. twenty-five percent of cases were not diagnosed until autopsy. maternal and perinatal mortality are high, approaching 39 percent for both mother and ... | 1999 | 10394584 |
| [the significance of determining antibodies to viruses of the herpesviridae family in rheumatic diseases]. | assay of antibodies to cytomegalovirus (cmv), herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) and epstein-barr virus (ebv) in rheumatic patients. specification of their correlations with clinical symptoms. | 1999 | 10399234 |
| neutrophils aid in protection of the vaginal mucosae of immune mice against challenge with herpes simplex virus type 2. | large numbers of polymorphonuclear leukocytes (pmns) infiltrated the murine vaginal mucosa within 24 h after intravaginal inoculation with an attenuated strain of herpes simplex virus type 2 (hsv-2). the role of these cells in resolution of a primary genital infection and in protection of hsv-immune animals against challenge with a fully virulent hsv-2 strain was investigated. depletion of greater than 95% of the pmns at the vaginal mucosal surface prior to intravaginal inoculation with an atten ... | 1999 | 10400730 |
| herpes simplex virus latency-associated transcript encodes a protein which greatly enhances virus growth, can compensate for deficiencies in immediate-early gene expression, and is likely to function during reactivation from virus latency. | herpes simplex virus types 1 and 2 (hsv1 and hsv2) enter and reactivate from latency in sensory neurons, although the events governing these processes are little understood. during latency, only the latency-associated transcripts (lats) are produced. however, although the lat rnas were described approximately 10 years ago, their function remains ambiguous. mutations affecting the lats have minimal effects other than a small reduction in establishment of and reactivation from latency in some case ... | 1999 | 10400759 |
| modified vp22 localizes to the cell nucleus during synchronized herpes simplex virus type 1 infection. | the ul49 gene product (vp22) of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) is a virion phosphoprotein which accumulates inside infected cells at late stages of infection. we previously (j. a. blaho, c. mitchell, and b. roizman, j. biol. chem. 269:17401-17410, 1994) discovered that the form of vp22 packaged into infectious virions differed from vp22 extracted from infected-cell nuclei in that the virion-associated form had a higher electrophoretic mobility in denaturing gels. based on t ... | 1999 | 10400775 |
| intranasal immunization with recombinant gd2 reduces disease severity and mortality following genital challenge with herpes simplex virus type 2 in guinea pigs. | the ability of a genetically detoxified mutant of heat labile enterotoxin (ltk63) to act as a mucosal adjuvant following intranasal immunization with recombinant gd2 has previously been reported in mice [ugozzoli m, o'hagan dt, ott gs. intranasal immunization of mice with herpes simplex virus type 2 recombinant gd2: the effect of adjuvants on mucosal and serum antibody responses. immunol 1998;93:563-571.]. in the current studies, these observations were extended to the guinea pig model. immunize ... | 1999 | 10403590 |
| antimicrobial activity of extracts from the cell cultures of some turkish medicinal plants. | twenty-four callus, and eleven cell suspension, cultures were established from turkish medicinal plants, and crude extracts prepared from them tested against microorganisms to assess their antimicrobial activities in vitro. of the extracts tested, those belonging to the cell cultures of five of the plant species showed antibacterial activity against mainly three bacteria and a yeast. no activity was observed against herpes simplex viruses, hsv-i and ii, but an extract from hypericum capitatum sh ... | 1999 | 10404549 |
| typing of clinical herpes simplex virus type 1 and type 2 isolates with monoclonal antibodies. | the purpose of this study was to evaluate the performance of a herpes simplex virus (hsv) type 1-specific anti-glycoprotein c-1 monoclonal antibody (mab) and a type 2-specific anti-glycoprotein g-2 mab for typing of 2,400 clinical hsv-1 isolates and 2,400 clinical hsv-2 isolates, respectively, using an enzyme immunoassay. the anti-hsv-1 mab showed sensitivity and specificity of 100%, and the anti-hsv-2 mab showed a sensitivity of 99.46% and 100% specificity, indicating that these mabs are suitab ... | 1999 | 10405430 |
| analysis of herpes simplex virus 1 and 2 infection in women with high risk sexual behaviour in mexico. | this paper describes the seroprevalence and risk factors of herpes simplex virus (hsv) infection in a group of female prostitutes from mexico city. | 1999 | 10405866 |
| evaluation of the antiviral activity of natural sulfated polyhydroxysteroids and their synthetic derivatives and analogs. | disodium 3beta,21-dihydroxypregn-5-en-20-one disulfate (2), sodium 3beta,21-dihydroxypregn-5-en-20-one 3-sulfate (3), sodium 3beta,21-dihydroxypregn-5-en-20-one 21-sulfate (4), and disodium 3beta,6alpha-dihydroxy-5alpha-pregnan-20-one disulfate (6) have been synthesized and completely characterized for the first time from readily available materials. sulfation was performed using triethylamine-sulfur trioxide complex in dimethylformamide as the sulfating agent. selective sulfation of 3beta,21-di ... | 1999 | 10406483 |
| aseptic herpetic meningitis: an uncommon genital herpes sequelae. | aseptic herpetic meningitis is a clinical syndrome characterized by fever, headaches, confusion, and a combination of meningeal signs. the spinal fluid findings consist of an increase in mononuclear cells (mononuclear pleocytosis), increased protein concentration, and normal glucose concentrations. aseptic herpetic meningitis is thought to be caused by a viral infection, although the specific virus is usually not demonstrated. the condition is self-limited and requires no treatment. | 1999 | 10412172 |
| strategies to control the genital herpes epidemic. | genital herpes is a widely prevalent sexually transmitted disease. we introduce a spatial stochastic model to discuss strategies to control the epidemic. our caricatural description suggests three different directions in order to control the epidemic. first, if the transmission rate is low enough there can be no epidemic. second, even for a high transmission rate the epidemic will be avoided if the mean time a viral outbreak lasts in a patient is short enough. finally, the mean time between two ... | 1999 | 10414029 |
| [antibodies against herpes simplex virus type 2 among pregnant women in norway]. | the prevalence of antibodies against herpes simplex virus type 2 (anti-hsv-2) among pregnant women in norway is not known. to study the prevalence of anti-hsv-2, a random sample of 961 women was drawn from a study population of 35,940 pregnant women in norway during 1992-94. 27% (256/961) had anti-hsv-2. the prevalence increased with age. 17% of the 20-24-year-olds and 34% of the 35 year-old or older had anti-hsv-2. the presence of antibodies also varied geographically, from 18% in the south to ... | 1999 | 10414195 |
| correlates of herpes simplex virus seroprevalence among women attending a sexually transmitted disease clinic. | infections by herpes simplex viruses type 1 (hsv-1) and type 2 (hsv-2) are common in the united states. herpes simplex virus type 2 is transmitted sexually, and the prevalence of antibodies to hsv-2 has increased in recent years. goals of this study: the objective of the present study was to estimate the seroprevalence of hsv-1 and hsv-2 antibodies among women attending a sexually transmitted disease (std) clinic and to evaluate factors associated with hsv-1 and hsv-2 seropositivity. | 1999 | 10417020 |
| identification of type-specific domains within glycoprotein g of herpes simplex virus type 2 (hsv-2) recognized by the majority of patients infected with hsv-2, but not by those infected with hsv-1. | a combination of phage peptide display library mapping and pepscanning, with both murine monoclonal antibodies and a panel of well-characterized human sera, have been used in order to define type-specific epitopes of glycoprotein g of herpes simplex virus type 2 (hsv-2) (gg2). both techniques revealed an immunodominant region of gg2, centred around amino acids 525-587 of the uncleaved gg2 molecule. a soluble peptide, equivalent to amino acids 551-570, when used as antigen in an elisa format was ... | 1999 | 10423148 |
| nucleosides and nucleotides. 185. synthesis and biological activities of 4'alpha-c-branched-chain sugar pyrimidine nucleosides. | a series of 4'alpha-c-branched-chain pyrimidine nucleosides was synthesized from 2'-deoxycytidine or uridine. in the 2'-deoxycytidine series, the substituent at the 4'alpha-position affected cytotoxicity against l1210 mouse leukemic cells in vitro in the order me (23) > cn (22) > c(symbol)ch (21) > ch=ch(2) (19) > et (24) > ch=chcl (20). however, uridine and cytidine derivatives with ethynyl and cyano groups at the 4'alpha-position did not show any cytotoxicity. the antiviral activities of these ... | 1999 | 10425099 |
| mhc-peptide ligand interactions establish a functional threshold for antigen-specific t cell recognition. | antigen-specific t cell recognition is dependent on the functional density of the tcr-ligand, which consists of specific mhc molecules and a specifically bound peptide. we have examined the influence of the affinity and concentration of exogenous peptide and the density of specific mhc molecules on the proliferation of a cd4+, dqa1*0501/dqb1*0201 (dq2.1)-restricted, hsv-2-specific t cell clone. using antigen peptide analogs with different mutations of known dq2-anchor residues, t cell response w ... | 1999 | 10426278 |
| recombinant glycoprotein vaccine for the prevention of genital hsv-2 infection: two randomized controlled trials. chiron hsv vaccine study group. | in the last 3 decades, herpes simplex virus type 2 (hsv-2) infection seroprevalence and neonatal herpes have increased substantially. an effective vaccine for the prevention of genital herpes could help control this epidemic. | 1999 | 10432030 |
| herpes simplex virus vaccines--why don't antibodies protect? | 1999 | 10432037 | |
| synthesis and antiviral activity of l-2'-deoxy-2'-up-fluoro-4'-thionucleosides. | l-2'-deoxy-2'-up-fluoro-4'-thionucleosides were efficiently synthesized from d-xylose via l-4-thioarabitol derivative as a key intermediate and evaluated for antiviral activities against hiv-1, hsv-1,2 and hbv. | 1999 | 10432648 |
| synthesis of nucleosides having unusual branched sugars as potential antiviral agents. | enantiomerically pure novel nucleosides having unusual branched sugars were synthesized in a stereospecific manner from a common chiral pool of (s, s)-1,4-bis(benzyloxy)-2,3-epoxybutane and evaluated for antiviral activity. | 1999 | 10432661 |
| serologic evidence of human herpesvirus 8 transmission by homosexual but not heterosexual sex. | epidemiologic studies link kaposi's sarcoma with a sexually transmitted agent. human herpesvirus 8 (hhv-8) is likely to be that agent, but routes of transmission are poorly described. a seroepidemiologic study was conducted to determine whether hhv-8 is transmitted sexually between heterosexuals. sera from 2718 patients attending a sexually transmitted disease (std) clinic were tested for antibodies to hhv-8 and herpes simplex virus type 2 (hsv-2). information on sex partners in the previous 12 ... | 1999 | 10438345 |
| antiviral activity of south brazilian medicinal plant extracts. | brazilian plants are potential sources of useful edible and medicinal plants. hydromethanolic extracts prepared from 54 medicinal plants used in folk medicine to treat infections were screened for antiviral properties against five different viruses (hsv-1, hsv-2, poliovirus type 2, adenovirus type 2 and vsv). fifty-two percent of the plant extracts exhibited antiviral against one or more tested viruses. more specifically, 42.6% showed activity against hsv-1 (herpes simplex virus type 1), 42.6% a ... | 1999 | 10439486 |
| a duplex pcr assay for detection and genotyping of herpes simplex virus in cerebrospinal fluid. | a duplex polymerase chain reaction (pcr) assay for the detection and genotyping of herpes simplex virus (hsv) 1 and 2 from cerebrospinal fluid (cfs) of infants was developed. the glycoprotein d (gd) gene of hsv was selected as a target for amplification. the assay is highly specific, sensitive and reproducible. herpes simplex virus detection is performed by agarose gel electrophoresis and southern blot using a chemiluminescent probe. the probe hybridizes to sequences common to both hsv-1 and 2. ... | 1999 | 10441204 |
| design of a "microbicide" for prevention of sexually transmitted diseases using "inactive" pharmaceutical excipients. | the human immunodeficiency virus (hiv-1) pandemic has been driven primarily by the sexual transmission of the virus, and facilitated by prior infections with other sexually transmitted disease (std) pathogens. although treatment of these stds has been proposed as a means to decrease the rate of hiv-1 sexual transmission, preventive measures effective against both hiv-1 and other std pathogens are expected to have a larger impact. these measures include topically applied mechanical and chemical ( ... | 1999 | 10441398 |
| plant products as topical microbicide candidates: assessment of in vitro and in vivo activity against herpes simplex virus type 2. | there is considerable interest in developing topical microbicides; products to be used intravaginally by women for protection against sexually transmitted diseases. many compounds derived from plants have been shown to have antimicrobial properties. we examined 19 such compounds in vitro by plaque reduction assay to determine their activity against a common sexually transmitted pathogen, herpes simplex virus type 2. compounds with an ed50 < or = 7.0 mg/ml were tested for efficacy in vivo. four c ... | 1999 | 10443534 |
| antitumor and antiviral activity of colombian medicinal plant extracts. | extracts of nine species of plants traditionally used in colombia for the treatment of a variety of diseases were tested in vitro for their potential antitumor (cytotoxicity) and antiherpetic activity. mtt (tetrazolium blue) and neutral red colorimetric assays were used to evaluate the reduction of viability of cell cultures in presence and absence of the extracts. mtt was also used to evaluate the effects of the extracts on the lytic activity of herpes simplex virus type 2 (hsv-2). the 50% cyto ... | 1999 | 10446015 |
| successful treatment of generalized primary herpes simplex type 2 infection during pregnancy. | generalized herpes simplex virus infections constitute a severe threat to mother and child during pregnancy. before antiviral treatments were available, both mother and foetus had a high mortality rate. the following case illustrates a successful outcome after long-term antiviral treatment. it is of great importance to suspect, diagnose and treat patients with hsv 2 early, especially during pregnancy. | 1999 | 10447333 |