Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the active site of icp47, a herpes simplex virus-encoded inhibitor of the major histocompatibility complex (mhc)-encoded peptide transporter associated with antigen processing (tap), maps to the nh2-terminal 35 residues. | the herpes simplex virus (hsv) immediate early protein icp47 inhibits the transporter associated with antigen processing (tap)-dependent peptide translocation. as a consequence, empty major histocompatibility complex (mhc) class i molecules are retained in the endoplasmic reticulum and recognition of hsv-infected cells by cytotoxic t lymphocytes is abolished. we chemically synthesized full-length icp47 (sicp47) and show that sicp47 inhibits tap-dependent peptide translocation in human cells. its ... | 1997 | 9151894 |
| localization of latency-associated transcripts in the uterovaginal plexus of herpes simplex virus type 1 and 2 latently infected mice. | the vagina and medulla of the adrenal gland of mice vaginally infected with herpes simplex virus (hsv) types 1 and 2 were examined in the latent stage of infection (5 to 51 weeks post-infection). rna in situ hybridization with hsv-1 and -2 latency-associated transcript (lat) rna probes resulted in positively stained neuronal cell nuclei in the uterovaginal plexus, but not in the medulla of the adrenal gland. these organs were chosen because hsv antigens can be detected not only in the vaginal ep ... | 1997 | 9152429 |
| treatment of spontaneously arising retinoblastoma tumors in transgenic mice with an attenuated herpes simplex virus mutant. | the use of viruses to treat tumors has received renewed interest with the availability of genetically defined attenuated mutants. herpes simplex virus (hsv) type 1 in particular has been shown to be effective for tumors of neuronal origin. however, the model systems used for these studies rely on the use of explanted tumor cells in immunodeficient animals. we have used a recently developed transgenic mouse model, wherein mice spontaneously develop retinoblastomas, to determine if a mutant hsv ha ... | 1997 | 9123872 |
| preferential presentation of herpes simplex virus t-cell antigen by hla dqa1*0501/dqb1*0201 in comparison to hla dqa1*0201/dqb1*0201. | the hla dqa1 locus is polymorphic. haplotypes containing hla dqa1*0501, but not hla dqa1*0201, together with hla dqb1*0201 are associated with grave's disease and celiac sprue. in this report, we demonstrate a functional correlate of dqa1 polymorphism. t cells infiltrating a herpes simplex virus (hsv) lesion from a hla dq 2,7 individual yielded a virus-specific cd4+ clone restricted by dq2. presentation of viral peptide and protein segregated with dqa1 allele, because cell lines bearing dqa1*050 ... | 1997 | 9129979 |
| pleural effusion due to herpes simplex type ii infection in an immunocompromised host. | pleural effusions caused by herpes simplex viruses are rare. we report a case of a young woman with acute lymphocytic leukemia (all) and prolonged neutropenia who developed pleural space infection with herpes simplex type ii virus (hsv ii), as confirmed by cytologic and microbiologic studies. we believe that this is the first report of a pleural effusion caused by hsv ii, and suggest that this virus now be considered in the differential diagnosis of an unexplained exudative pleural effusion, esp ... | 1997 | 9001338 |
| current clinical issues in the management of herpes simplex virus infections in patients with hiv. | a recent study done in baltimore showed hsv-2 seroprevalence of 81% among 64 hiv-positive homosexual or bisexual men. | 1997 | 9094453 |
| specificity and affinity of binding of herpes simplex virus type 2 glycoprotein b to glycosaminoglycans. | herpes simplex virus type 2 (hsv-2) interacts with cell surface glycosaminoglycans during virus attachment. glycoprotein b of hsv-2 can potentially mediate the interaction between the virion and cell surface glycosaminoglycans. to determine the specificity, kinetics, and affinity of these interactions, we used plasmon resonance-based biosensor technology to measure hsv-2 glycoprotein binding to glycosaminoglycans in real time. the recombinant soluble ectodomain of hsv-2 gb (gb2) but not the solu ... | 1997 | 8995662 |
| seroprevalence of herpes simplex virus types 1 and 2 and cytomegalovirus in adolescents. | we determined the prevalence of herpes simplex virus (hsv) and cytomegalovirus (cmv) antibodies in a cohort of adolescents 12 to 22 years of age in anticipation of the development of vaccines to control hsv and cmv infections. for the overall study population, we found that 62% were seropositive for hsv type 1 (hsv-1), 12% were seropositive for hsv type 2 (hsv-2), and 65% were seropositive for cmv. race was not related to hsv-1 seropositivity, but african-american adolescents were more likely th ... | 1997 | 9114136 |
| asymptomatic perianal shedding of herpes simplex virus in patients with acquired immunodeficiency syndrome. | to determine the frequency of asymptomatic perianal shedding of herpes simplex virus (hsv) in adult patients with acquired immunodeficiency syndrome (aids). | 1997 | 9041831 |
| [construction of recombinant vaccinia virus expressing hsv-2 gd gene as live recombinant vaccine strain]. | we had reported that the recombinant vaccinia virus expressing glycoprotein d of herpes simplex virus type 2 (hsv-2 gd) protected mice against lethal hsv-2 challenge. following the succeed in animal model, we continue the research to construct the recombinant vaccinia virus expressing hsv-2 gd gene as live recombinant vaccine strain in strict accordance with the guideline for human vaccine research. a pcr-modified hsv-2 gd gene was inserted into the plasmid pjsb1175, under the control of p7.5k e ... | 1997 | 15619895 |
| prevalence of transfusion associated infections in multitransfused children in relation to mandatory screening of hiv in donated blood. | any change in risk behavior related to acquisition of human immunodeficiency virus (hiv) infection is likely to reduce simultaneously the risk for other agents transmitted through identical routes. a study carried out in the city of delhi, india on the load of transfusion associated infections among multitransfused (mt) children in relation to mandatory screening of hiv infection in donated blood indicated unchanged prevalence of hepatitis b virus (hbv), hepatitis c virus (hcv) and hepatitis d v ... | 1997 | 9656388 |
| [detecting pathogens of fetal defects in paraffin embedded tissues by polymerase chain reaction]. | to examine 3 types of pathogens in dead fetus with congenital defects for exploring the pathogenesis. | 1997 | 9639727 |
| dna increases the potency of vaccination against infectious diseases. | in the past couple of years, the idea that naked dna can be used to vaccinate against infections has been rapidly developing. in contrast to traditional protein or live attenuated vaccines, there is no risk of disease caused by dna vaccines as only selected proteins are encoded. the ease with which dna may be manipulated means that vaccines can be custom designed to meet many needs. in animal model systems, dna vaccines have proved to be as effective as traditional vaccines. additionally, this t ... | 1997 | 9667856 |
| characterization of t cell responses to herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) using a tnf-beta elispot cytokine assay. | we examined the suitability of a tnf-beta cytokine elispot assay for assessing various aspects of the t cell response to herpes simplex viruses. the number of t cells responding to hsv-1 or hsv-2 was measured by tnf-beta elispot assay. the number of t cells producing tnf-beta in response to hsv-1 was high, ranging from 76 to 222 per 10(5). hsv-1-specific tnf-beta-secreting responder cell frequencies fluctuated over time in individual donors. comparable fluctuations were not observed in the t cel ... | 1997 | 9672626 |
| [monoclonal antibodies which recognize herpes simplex virus and their possible diagnostic application]. | it is reported the obtention of monoclonal antibodies, which are capable of recognizing hsv-1 and hsv-2 viruses, by the conventional technology of cellular fusion. the monoclonal antibodies of highest positivity according to the indirect immunofluorescence technique were partially characterized. it was determined the neutralizing capacity of 2 of them (278-f7 and 70-g10), which were used to identify isolations of clinical specimens by indirect immunofluorescence. these monoclonal antibodies reco ... | 1997 | 9685985 |
| genital herpes: treatment guidelines. | genital herpes, usually caused by herpes simplex virus type 2 (hsv-2), is the most common cause of genital ulceration. the primary episode of genital herpes is generally the most painful. subsequent recurrences are generally milder and localized. diagnosis is made clinically, but should be confirmed by culture or serology. management includes antiviral drug therapy--acyclovir, valacyclovir, or famciclovir--as well as analgesics. in addition, patient counseling and education are vital. antiviral ... | 1997 | 9746692 |
| genital herpes: recognizing the problem. | genital herpes, usually the result of an infection with herpes simplex virus type 2 (hsv-2), is the most common cause of genital ulceration. the first clinical episode is called primary genital herpes; recurrent hsv infection occurs in up to 80% of patients. because not every individual who acquires the virus develops symptoms, it is difficult to estimate the prevalence of hsv infection. transmission of virus occurs not only in the presence of apparent lesions, but asymptomatic viral shedding ca ... | 1997 | 9746717 |
| [herpetic gingivostomatitis on the basis of infection with hsv type 2]. | 1997 | 11923919 | |
| herpes study and resources. | herpes is caused by a virus that causes recurring bouts of cold sores or genital lesions. the differences between herpes simplex virus 1 (hsv-1) and herpes simplex virus 2 (hsv-2) are explained. herpes outbreaks can become harder to treat when the immune system is damaged by hiv. acyclovir and famciclovir are safe and effective treatments, but preventing infection is especially important in hiv-infected individuals, as the amount of hiv in the blood increases during a herpes outbreak. world wide ... | 1997 | 11364615 |
| hsv-2 and hiv: consequences of an endemic opportunistic infection. | people with hiv are also frequently co-infected with herpes simplex viruses (hsv), although the interactions between the two are not fully understood. evidence suggests that hsv is a risk factor for the transmission of hiv, is a common opportunistic pathogen in hiv-infected persons, and that hsv reactivation appears to regulate hiv replication. the clinical significance of these interactions is not well defined. current information about the hsv-hiv interaction is reviewed, and future research p ... | 1997 | 11364936 |
| herpes simplex virus 2 causes apoptotic infection in monocytoid cells. | increasing evidence indicates that apoptosis can be associated with several viral infections. here we demonstrate, that infection of monocytoid cells by herpes simplex virus 2 (hsv-2) resulted, in time- and dose-dependent induction of apoptosis as an exclusive cytopathic effect. the phenomenon was confirmed using four different techniques. conversely, apoptosis was not observed in the vero cell line. virus yield in monocytoid cells was delayed and reduced, although well detectable, in comparison ... | 1997 | 14555977 |
| cdc studies link between herpes and hiv prevention. centers for disease control and prevention. | the centers for disease control and prevention (cdc) convened a group of consultants to study the link between genital herpes (hsv-2) and hiv. there are high numbers of herpes cases reported in the united states, and accurate, type-specific assays are now available to determine whether asymptomatic people are virus carriers. the prevalence of hsv-2 in the hiv epidemic is thought to be greatest among heterosexuals, with infected persons being twice as likely to acquire hiv. the cdc is urged to co ... | 1998 | 11365590 |
| [the relationship between herpes simplex virus ii, human papillomavirus infection and infertility after artificial abortion]. | in order to study the relationship between infection of sexually transmitted virus herpes simplex virus ii (hsv2), human papillomavirus (hpv) and female infertility after artificial abortion, we collected 60 genital samples from infertile women who had accepted artificial abortions and 39 genital samples from normal women. polymerase chain reaction (pcr) was used to detect hsv2 and hpv. the results were compared by x2. the positive rate of hsv2 in infertile and normal women were 80.0% and 25.6% ... | 1998 | 12515196 |
| a cell cycle model for the tachyzoite of toxoplasma gondii using the herpes simplex virus thymidine kinase. | toxoplasma gondii (rh strain) tachyzoites were transfected with a plasmid containing a fusion of the chloramphenicol acetyl transferase and the herpes simplex virus-2 thymidine kinase coding regions and transgenic parasites obtained by chloramphenicol selection. ctk11, a single high expressing clone was isolated based on immunofluorescence and contained approximately five integrated copies of the fusion sequence. lysates prepared from this clone displayed thymidine kinase activity of 2.9 pmol mi ... | 1998 | 9747974 |
| the antiherpesvirus activity of h2g [(r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine] is markedly enhanced by the novel immunosuppressive agent mycophenolate mofetil. | mycophenolate mofetil (mmf) has been approved as an immunosuppressive agent in kidney transplant recipients and may thus be used concomitantly with antiherpetic agents, which are used for the treatment of intercurrent herpesvirus infections. we have recently demonstrated that mmf and its parent compound mycophenolic acid (mpa), which is a potent inhibitor of imp dehydrogenase, potentiate the antiherpesvirus activity of acyclovir, ganciclovir, and penciclovir. we have now evaluated the antiviral ... | 1998 | 9835529 |
| 5-substituted pyrimidines with a 1,5-anhydro-2, 3-dideoxy-d-arabino-hexitol moiety at n-1: synthesis, antiviral activity, conformational analysis, and interaction with viral thymidine kinase. | a new series of anhydrohexitol nucleosides are described. these compounds have a pyrimidine base moiety substituted in the 5-position with a chloro (1b), trifluoromethyl (1c), vinyl (1d), 2-thienyl (1e), ethynyl (1f) or propynyl (1g) substituent. the vinyl, propynyl, and, in particular, the 5-trifluoromethyl analogue showed potent activity against herpes simplex virus (hsv), 1c with a selectivity index of >16000 against hsv-1 and >1000 against hsv-2. conformational analysis of anhydrohexitol nuc ... | 1998 | 9784109 |
| [structural organization of 2c-terminal region and its analogs in a subunit of ribonucleotide reductase from herpes virus]. | by means of conformational analysis, the spatial structure and conformational potential of the h-tyr-ala-gly-ala-val-val-asn-asp-leu-oh molecule, which corresponds to sequence 329-337 of the subunit 2 c-terminal region of the herpes virus ribonucleotide reductase, were studied. it was shown that its spatial organization can be described by a set of 17 low-energy conformations of the backbone. the "reverse conformational problem" for this molecule was solved to enable the prediction of a series o ... | 1998 | 9784878 |
| herpes simplex virus type 2 dna detected in cerebrospinal fluid of 9 patients with mollaret's meningitis. | we present clinical and virological data on 9 patients, 7 women and 2 men aged 31-56 years, with recurrent aseptic meningitis (mollaret's meningitis). polymerase chain reaction detected herpes simplex virus type 2 dna in cerebrospinal fluid samples from all patients collected during their latest attacks of meningitis. six patients had no history of genital herpes. only 1 patient was offered prophylactic antiviral treatment during the study period (45 months). | 1998 | 9786620 |
| pharmacokinetics of oral valacyclovir and acyclovir in late pregnancy. | the objective was to obtain preliminary pharmacokinetic data for acyclovir from gravid women receiving herpes simplex virus suppressive therapy with the acyclovir prodrug valacyclovir. | 1998 | 9790357 |
| a comparison of biodegradable microparticles and mf59 as systemic adjuvants for recombinant gd from hsv-2. | a recombinant form of glycoprotein d from herpes simplex virus type-2 (gd2) was encapsulated into polylactide-co-glycolide (plg) microparticles using a previously established solvent evaporation technique. the mean size of the microparticles was about 1 micron and high encapsulation efficiency of the antigen was achieved (70-80%). the microparticles were administered intramuscularly to balb/c mice and the immune responses were compared with those obtained with the oil in water adjuvant mf59. the ... | 1998 | 9795387 |
| briarane diterpenes from the indian ocean gorgonian gorgonella umbraculum. | three briarane diterpenes, junceellin (1), praelolide (2), and compound 3 (of which 3 is new), were isolated from the gorgonian gorgonella umbraculum (eii & sol), and their structures were established on the basis of their spectral data. | 1998 | 9748378 |
| suppression of recurrent genital herpes simplex virus type 2 infection by rhus javanica in guinea pigs. | rhus javanica has been shown to exhibit anti-herpes simplex virus (hsv) activity and potentiate the anti-hsv activity of acyclovir in vitro and in vivo. this extract was examined for its suppressive efficacy on recurrent genital infection in guinea pigs. guinea pigs were primarily infected intravaginally with hsv type 2 (hsv-2). prophylactic oral administration, at the dose corresponding to human use, of r. javanica significantly reduced the incidence, severity and/or frequency of spontaneous an ... | 1998 | 9754947 |
| antiviral activity of viracea against acyclovir susceptible and acyclovir resistant strains of herpes simplex virus. | viracea, a topical microbicide, is a blend of benzalkonium chloride and phytochemicals derived from echinacea purpurea and is a proprietary formula from destiny biomedix corp. viracea was tested against 40 strains of herpes simplex virus (hsv): 15 strains (five hsv-1 and ten hsv-2) were resistant to acyclovir (acv-r) and 25 strains (13 hsv-1 and 12 hsv-2) were susceptible to acv (acv-s). the median ed50 of viracea for the five acv-r strains of hsv-1 was a 1:100 dilution of the drug with a range ... | 1998 | 9754950 |
| structural basis of specificity and degeneracy of t cell recognition: pluriallelic restriction of t cell responses to a peptide antigen involves both specific and promiscuous interactions between the t cell receptor, peptide, and hla-dr. | tcr engagement of peptide-mhc class ii ligands involves specific contacts between the tcr and residues on both the mhc and peptide molecules. we have used molecular modeling and assays of peptide binding and t cell function to characterize these interactions for a cd4+ th1 cell clone, esl4.34, which recognizes a peptide epitope of the herpes simplex type 2 virus virion protein, vp16 393-405, in the context of several hla-dr alleles. this clone responded to vp16 393-405 in proliferation and cytot ... | 1998 | 9759873 |
| frequency of symptomatic and asymptomatic herpes simplex virus type 2 reactivations among human immunodeficiency virus-infected men. | herpes simplex virus (hsv) infection is common in persons coinfected with human immunodeficiency virus (hiv). in a prospective study, daily viral cultures of the mouth, genitals, and rectum were collected from 68 hiv-positive and 13 hiv-negative men who have sex with men. subjects completed a median of 57 days of follow-up. anogenital hsv-2 cultures were positive on 405 (9.7%) of 4167 days for hiv-positive men and on 24 (3.1%) of 766 days for hiv-negative men. most reactivations were perirectal ... | 1998 | 9815213 |
| herpes simplex virus shedding among human immunodeficiency virus-negative men who have sex with men: site and frequency of shedding. | the frequency and anatomic location of subclinical and symptomatic herpes simplex virus (hsv) shedding were evaluated among human immunodeficiency virus (hiv)-negative hsv-2-seropositive men who have sex with men (msm). eight men attended a research clinic daily for 30 days for a detailed genital examination and anoscopy with colposcopy to detect herpes lesions. hsv cultures were obtained daily from four sites (perianal, urethral, penile shaft, and oral) at home and the research clinic. signs an ... | 1998 | 9806024 |
| herpes simplex virus as a transneuronal tracer. | determining the connections of neural systems is critical for determining how they function. in this review, we focus on the use of hsv-1 and hsv-2 as transneuronal tracers. using hsv to examine neural circuits is technically simple. hsv is injected into the area of interest, and after several days, the animals are perfused and processed for immunohistochemistry with antibodies to hsv proteins. variables which influence hsv infection include species of host, age of host, titre of virus, strain o ... | 1998 | 9809305 |
| differential intracellular compartmentalization of herpetic thymidine kinases (tks) in tk gene-transfected tumor cells: molecular characterization of the nuclear localization signal of herpes simplex virus type 1 tk. | the thymidine kinases (tks) of herpes simplex virus type 1 (hsv-1), hsv-2, and varicella-zoster virus (vzv) were expressed in human osteosarcoma cells as fusion proteins with the green fluorescent protein (gfp), and their intracellular localizations were determined. the three tk-gfp fusion products were localized in different subcellular compartments of the transfected tumor cells. hsv-1 tk-gfp was localized exclusively in the nucleus, hsv-2 tk-gfp was predominantly found in the cytosol, while v ... | 1998 | 9811686 |
| immunization with a single major histocompatibility complex class i-restricted cytotoxic t-lymphocyte recognition epitope of herpes simplex virus type 2 confers protective immunity. | we have evaluated the potential of conferring protective immunity to herpes simplex virus type 2 (hsv-2) by selectively inducing an hsv-specific cd8(+) cytotoxic t-lymphocyte (ctl) response directed against a single major histocompatibility complex class i-restricted ctl recognition epitope. we generated a recombinant vaccinia virus (rvv-es-gb498-505) which expresses the h-2kb-restricted, hsv-1/2-cross-reactive ctl recognition epitope, hsv glycoprotein b residues 498 to 505 (ssiefarl) (gb498-505 ... | 1998 | 9811690 |
| the ectodomain of a novel member of the immunoglobulin subfamily related to the poliovirus receptor has the attributes of a bona fide receptor for herpes simplex virus types 1 and 2 in human cells. | we report on the functional cloning of a hitherto unknown member of the immunoglobulin (ig) superfamily selected for its ability to confer susceptibility to herpes simplex virus (hsv) infection on a highly resistant cell line (j1.1-2 cells), derived by exposure of bhktk- cells to a recombinant hsv-1 expressing tumor necrosis factor alpha (tnf-alpha). the sequence of herpesvirus ig-like receptor (higr) predicts a transmembrane protein with an ectodomain consisting of three cysteine-bracketed doma ... | 1998 | 9811737 |
| treatment of acyclovir-resistant herpetic ulceration with topical foscarnet and antiviral sensitivity analysis. | herpes simplex virus (hsv) can produce persistent mucocutaneous disease in patients with the acquired immunodeficiency syndrome (aids). in this case report, we evaluate the efficacy, safety and viral resistance after topical foscarnet in severe genital ulceration due to acyclovir-resistant hsv-2. | 1998 | 9812037 |
| carrageenan-based nonoxynol-9 spermicides for prevention of sexually transmitted infections. | carrageenan-based nonoxynol-9 (n-9)-containing formulations were developed in response to the concern that over the counter (otc) spermicides may not protect people from hiv infection and other sexually transmitted pathogens. | 1998 | 9800263 |
| incidence of hiv and sexually transmitted diseases (std) in a cohort of hiv-negative men who have sex with men (msm). | to determine the prevalence of sexually transmitted diseases (std) and incidence of and risk factors for std, including hiv-1, among a cohort of hiv-negative men who have sex with men (msm). | 1998 | 9814873 |
| characterization of the herpes simplex virus type 2 (hsv-2) us2 gene product and a us2-deficient hsv-2 mutant. | the herpes simplex virus type 2 (hsv-2) us2 gene product was identified by using a rabbit polyclonal antiserum raised against a recombinant 6 x his-us2 fusion protein expressed in escherichia coli. the antiserum reacted specifically with a 39 kda protein in hsv-2 strain 186-infected cell lysates. the protein was not detectable in the presence of the virus dna synthesis inhibitor phosphonoacetic acid. indirect immunofluorescence studies localized the us2 protein in the cytoplasm and as discrete g ... | 1998 | 9820154 |
| performance of six cell lines in the suspension-infection test used for the detection of herpes simplex virus. | six cell lines were assessed in the suspension-infection (si) test for suitability for the rapid culture of herpes simplex virus (hsv). for the si test, the specimen was combined in growth medium with trypsinized, suspended culture cells before allowing the cells to settle into a monolayer growth pattern. the cells tested in the si assay were mv1-lu, vero c1008, bsc-1, rd, and mrc-9 cells. fifty clinical specimens composed of 7 hsv-1-positive samples, 9 hsv-2-positive samples, 12 positive for hs ... | 1998 | 9823529 |
| immunity to vaginal hsv-2 infection in immunoglobulin a knockout mice. | an immunoglobulin a (iga) knockout (ko) mouse was used to study the role of iga in protective immunity against vaginal infection with herpes simplex virus-type 2 (hsv-2). intact and ko mice were immunized intravaginally (ivag) with attenuated hsv-2, challenged ivag with wild-type virus 6 weeks later and evaluated for vaginal infection and neurological disease. non-immunized/challenged intact and ko mice showed vaginal infection and succumbed to neurological disease, while immunized/challenged mi ... | 1998 | 9824477 |
| sexually transmitted diseases and human immunodeficiency virus infection: cause, effect, or both? | the infectiousness of the index case and the susceptibility of the exposed host mediate the sexual transmission of human immunodeficiency virus (hiv). infectiousness of the index case is determined by the inoculum of hiv and viral factors that favor transmission. persons with primary infection, late stage disease, or low cd4 counts appear to transmit hiv with greater efficiency, presumably through increased viral burden in genital secretions. susceptibility to hiv infection is determined by here ... | 1998 | 9831668 |
| herpes simplex type ii and mycoplasma genitalium as risk factors for heterosexual hiv transmission: report from the heterosexual hiv transmission study. | two hundred twenty-four human immunodeficiency virus (hiv) discordant couples (one hiv negative, one hiv positive) were compared with 78 seroconcordant heterosexually infected couples with hiv with regard to sexually transmitted diseases. | 1998 | 9831669 |
| disinfection of eyelid speculums for retinopathy of prematurity examination. | to evaluate the effectiveness of 70% isopropyl alcohol swabs in disinfecting eyelid speculums after examination for retinopathy of prematurity. | 1998 | 9747678 |
| rd6-2198, a novel betain-type fluoroalkylated oligomer, inhibits the replications of human immunodeficiency virus type 1 and other enveloped viruses. | we have examined a novel betain-type fluoroalkylated oligomer, rd6-2198, for its inhibitory effects on the replication of human immunodeficiency virus type 1 (hiv-1) and other enveloped viruses, including herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) and respiratory syncytial virus (rsv) in cell cultures. we have found that the compound is a potent and selective inhibitor of these viruses. rd6-2198 inhibited the replication of hiv-1iiib at a concentration of 0.85 microg/ml w ... | 1998 | 9707376 |
| some aspects of the pathogenesis of hiv-1-associated kaposi's sarcoma. | kaposi's sarcoma (ks) is a very rare tumor except after human immunodeficiency virus type 1 (hiv-1) infection when it becomes common. most investigators assume that the role of hiv-1 is passive, i.e., via inducing immune deficiency, thereby enhancing cancer development, and specifically, in the case of human herpesvirus 8 (kshv) by enhancing hhv-8 replication. we suggest that the role of hiv-1 is more active in the disease process by at least two events: 1) promoting an increase in inflammatory ... | 1998 | 9709304 |
| pseudotumour of the tongue caused by herpes simplex virus type 2 in an hiv-1 infected immunosuppressed patient. | an hiv-1 infected immunosuppressed patient (cd4+ cell counts: 382 cells/microl; viral load 94,000 copies/ml) with recurrent perianal herpes simplex virus type 2 (hsv-2) infections is described, showing an unusual exophytic tumour resembling a squamous cell carcinoma in the lateral part of the tongue. he also had persistent facial herpes infection, oral candidosis, oral hairy leukoplakia and lymphadenopathy. the presence of hsv-2 was detected by polymerase chain reaction both in smears and in a t ... | 1998 | 9764162 |
| the pk domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is required for immediate-early gene expression and virus growth. | the large subunit of herpes simplex virus (hsv) ribonucleotide reductase (rr), rr1, contains a unique amino-terminal domain which has serine/threonine protein kinase (pk) activity. to examine the role of the pk activity in virus replication, we studied an hsv type 2 (hsv-2) mutant with a deletion in the rr1 pk domain (icp10deltapk). icp10deltapk expressed a 95-kda rr1 protein (p95) which was pk negative but retained the ability to complex with the small rr subunit, rr2. its rr activity was simil ... | 1998 | 9765459 |
| herpesvirus thymidine kinase transgenes that do not cause male sterility are aberrantly transcribed and translated in the testis. | mice that carry the wild-type herpes simplex virus type 1 (hsv1) thymidine kinase (tk) gene coupled to the bovine thyroglobulin (btg) promoter (btg-tk1 mice) express viral tk at a high level in the thyroid gland, and at an equally high level, ectopically, in the testis, which renders the males sterile. when the btg promoter was coupled either to a variant of hsv1-tk (differing from the wild type in 2 nucleotides) (btg-tk1alpha mice) or to the herpes simplex virus type 2 (hsv2) tk gene (btg-tk2 m ... | 1998 | 9767091 |
| genomic heterogeneity maps to tandem repeat sequences in the herpes simplex virus type 2 ul region. | two bam hi y and w fragments in the unique long sequence (ul) of the herpes simplex virus type 2 (hsv-2) were found to be heterogeneous in size among clones derived from a single strain as well as from epidemiologically unrelated isolates. more detailed restriction maps of these bamhi fragments were constructed and the heterogeneous subfragments were defined, cloned, and sequenced in order to investigate the mechanism causing the size difference. the subfragment of bamhi y contained a tandem rep ... | 1998 | 9725674 |
| detection of microorganisms in vessel wall specimens of the abdominal aorta: development of a pcr assay in the absence of a gold standard. | a procedure in which the "invitrogen easy-dna" kit was followed by a silica-based method for the isolation of dna was developed for extraction of pcr-inhibitor-free dna from up to 300 mg of human vessel wall tissue. optimally designed pcr assays were developed for the detection of at least one infected cell in this amount of tissue. details of the procedure are given for the detection of dna of chlamydia pneumoniae, cytomegalovirus and herpes simplex virus type 1 and type 2 in human vessel walls ... | 1998 | 9795995 |
| [recurrent mollaret's meningitis of herpetic origin]. | benign recurrent meningitis, or mollaret's meningitis, is an uncommon disease whose viral origin was long unidentified. since 1991, about twenty cases have been reported in patients with herpes infection. | 1998 | 9798461 |
| characterization of a type-common human recombinant monoclonal antibody to herpes simplex virus with high therapeutic potential. | we report the characterization of a type-common human recombinant monoclonal antibody previously isolated by antigen selection from a phage-displayed combinatorial antibody library established from a herpes simplex virus (hsv)-seropositive individual. competition with well-characterized murine monoclonal antibodies and immunodetection of gd truncations revealed that this antibody recognizes the group ib antigenic site of glycoprotein d, a highly conserved and protective type-common determinant. ... | 1998 | 9774565 |
| characterization of the ul4 gene product of herpes simplex virus type 2. | we have identified the herpes simplex virus type 2 (hsv-2) ul4 gene product using a rabbit polyclonal antiserum raised against a recombinant 6xhis-ul4 fusion protein expressed in escherichia coli. the antiserum reacted specifically with a 27-kda protein in hsv-2 186-infected cell lysates. the protein was not detectable in the presence of the viral dna synthesis inhibitor, suggesting that the ul4 gene was expressed as a gamma 2 gene. indirect immunofluorescence studies localized the ul4 protein w ... | 1998 | 9687876 |
| surveys of hiv-1, htlv-i, and other sexually transmitted diseases in female sex workers in taipei city, taiwan, from 1993 to 1996. | between 1993 and 1996 in taipei, taiwan, we studied the prevalences of hiv-1, htlv-i, hsv-2, chlamydia trachomatis, and syphilis infection in female commercial sex workers (csw) and the pathogens' associated risk factors. female csw from nine massage parlors, two karaoke bars (kb), and 22 brothels in taipei city participated in this study. two of 557 (0.4%) kb csws from those who participated during the period from 1993 to 1994 had hiv-1 infection, whereas no kb csws in the periods 1994 to 1995 ... | 1998 | 9665510 |
| rapid screening tests for determining in vitro susceptibility of herpes simplex virus clinical isolates. | the susceptibility of human herpes simplex virus (hsv) to acyclovir (acv) was determined with the use of a single dose of the drug (1 and 2 micrograms of acv per ml for hsv-1 and hsv-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (rapid cia) and a rapid dye uptake assay (rapid dua). these tests allow the simultaneous determination of virus titer and susceptibility to acv at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye ... | 1998 | 9666034 |
| nonoxynol-9 enhances rectal infection by herpes simplex virus in mice. | pretreating the rectum of mice with nonoxynol-9 (n9) or spermicides containing n9 before infecting via the rectal route with herpes simplex virus 2 (hsv-2) increased the likelihood of infection and shortened the time until infection. microscopic analysis indicated that n9 rapidly caused exfoliation of the rectal epithelium, exposing the underlying connective tissue. these findings suggest that use of n9-containing products during rectal intercourse may increase the risk of infection with hsv-2 o ... | 1998 | 9673842 |
| a review of famciclovir in the management of genital herpes. | the frequent occurrence of genital herpes continues to be a serious clinical problem. although not life threatening, the physical symptoms of the disease, and the ensuing psychosocial complications, can be overwhelming to patients. the life cycle of the herpes simplex virus is complex, comprising multiple stages. following infection, the virus establishes life-long latency in its host and can reactivate at any time as a recurrent infection. successful management of genital herpes simplex infecti ... | 1998 | 9678146 |
| functional analysis of the herpes simplex virus type 2 strain hg52 rl1 gene: the intron plays no role in virulence. | sequence analysis predicts that herpes simplex virus type 2 (hsv-2) strain hg52 contains an open reading frame, rl1, encoding a polypeptide equivalent to icp34.5 of hsv-1. similarly to hsv-1, deletion of the region spanning rl1 abolishes the virulence of hsv-2 strain hg52 and its ability to grow in stationary 3t6 cells. in contrast to hsv-1, the hsv-2 strain hg52 rl1 gene is predicted to contain a 154 bp intron. previously, we have demonstrated that this intron is spliced from rl1 poly(a)+ mrna ... | 1998 | 9680122 |
| antiviral activities of methylated nordihydroguaiaretic acids. 2. targeting herpes simplex virus replication by the mutation insensitive transcription inhibitor tetra-o-methyl-ndga. | we had previously reported that tetramethyl-o-ngda (m4n), a synthetic derivative of the naturally occurring nordihydroguaiaretic acid (ndga), is able to inhibit hiv tat transactivation by blocking host sp1 protein at the sp1 cognate binding site on the hiv ltr promoter. the present studies were undertaken to examine whether m4n is able to inhibit the replication of herpes simplex virus (hsv), another sp1-regulated virus. the results showed that in vero cells, m4n inhibits at micromolar levels (i ... | 1998 | 9685239 |
| low-productive alpha-herpesviridae infection in chronic lichenoid dermatoses. | herpes simplex virus (hsv) and varicella-zoster virus (vzv) are responsible for various atypical mucocutaneous manifestations in the immunosuppressed population. one of the causative pathomechanisms suggests an altered virus-host cell relationship. | 1998 | 9669126 |
| phenotypic and genotypic characterization of acyclovir-resistant herpes simplex viruses from immunocompromised patients. | phenotypic and genotypic analyses were done on 30 acyclovir-resistant and 5 acyclovir-susceptible herpes simplex virus (hsv) isolates (22 hsv type 1 and 13 hsv type 2) recovered from 24 subjects. all isolates were susceptible to foscarnet. the phenotypes of the acyclovir-resistant hsv isolates were as follows: 17 were thymidine kinase (tk) deficient, 12 had decreased tk activity (produced low amounts of viral tk) or tk with altered substrate specificity, and 1 was undetermined. sequencing analys ... | 1998 | 9697707 |
| identification of an immunodominant sequential epitope in glycoprotein g of herpes simplex virus type 2 that is useful for serotype-specific diagnosis. | a series of 67 oligopeptides that spanned the open reading frame of herpes simplex virus type 2 (hsv-2) glycoprotein g (gg2) were synthesized and tested for reactivity with 173 serum specimens collected from 117 individuals. the oligopeptides were made as multiple antigenic peptides consisting of four copies of a unique sequence attached to a branched lysine core and separated from the core by four glycine residues. the sera included hsv antibody-negative samples as well as sera from individuals ... | 1998 | 9700637 |
| detection of herpes simplex virus dna in human spermatozoa by in situ hybridization technique. | the role of herpes simplex virus (hsv) in male infertility has been investigated using the localizing ability of in situ hybridization technique. sperm samples obtained from 80 men attending a maternity center because of fertility problems were classified by spermogram and analyzed for the presence of hsv-1 and hsv-2 dna using digoxigenin-labelled dna probes. hsv dna was detected in the nuclei of spermatozoa in 37 semen samples (46%), hsv-1 specifically in 21 cases (26%) and hsv-2 in 16 cases (2 ... | 1998 | 9706490 |
| interferon alpha (ifn alpha 2a) therapy for herpes virus-associated inflammatory bowel disease (ulcerative colitis and crohn's disease). | the etiology and pathogenesis of ulcerative colitis and crohn's disease remain unclear, so that exact causal therapy is not yet possible. in our uc and cd patients, viral infections, particularly of the upper respiratory tract, aggravated the underlying disease. this had led us to use in-situ hybridization to investigate intestinal mucosa for viral agents such as hsv i + ii- and epstein-barr virus dna. we found these dna in the cell nuclei in the surface and glandular epithelia of the affected m ... | 1998 | 9684118 |
| [iatrogenic aseptic meningitis]. | 1998 | 9767811 | |
| inhibition of herpes simplex virus replication by a 2-amino thiazole via interactions with the helicase component of the ul5-ul8-ul52 complex. | with the use of a high-throughput biochemical dna helicase assay as a screen, t157602, a 2-amino thiazole compound, was identified as a specific inhibitor of herpes simplex virus (hsv) dna replication. t157602 inhibited reversibly the helicase activity of the hsv ul5-ul8-ul52 (ul5/8/52) helicase-primase complex with an ic50 (concentration of compound that yields 50% inhibition) of 5 microm. t157602 inhibited specifically the ul5/8/52 helicase and not several other helicases. the primase activity ... | 1998 | 9696789 |
| recognition of herpes simplex virus type 2 tegument proteins by cd4 t cells infiltrating human genital herpes lesions. | the local cellular immune response to herpes simplex virus (hsv) is important in the control of recurrent hsv infection. the antiviral functions of infiltrating cd4-bearing t cells may include cytotoxicity, inhibition of viral growth, lymphokine secretion, and support of humoral and cd8 responses. the antigens recognized by many hsv-specific cd4 t cells localizing to genital hsv-2 lesions are unknown. t cells recognizing antigens encoded within map units 0. 67 to 0.73 of hsv dna are frequently r ... | 1998 | 9696844 |
| herpes simplex virus infections of the central nervous system in human immunodeficiency virus-infected patients: clinical management by polymerase chain reaction assay of cerebrospinal fluid. | a duplex polymerase chain reaction (pcr) assay for dna from herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) was applied to cerebrospinal fluid (csf) from 918 human immunodeficiency virus (hiv)-infected patients with neurological symptoms. hsv-1 or hsv-2 (hsv-1/2) dna was found in 19 patients (2%). for the 258 patients for whom a diagnosis was confirmed at autopsy, the sensitivity and specificity of pcr analysis for the diagnosis of hsv-1/2 encephalitis were 100% and 99.6%, respectively. three ... | 1998 | 9709880 |
| atypical herpes simplex virus encephalitis diagnosed by pcr amplification of viral dna from csf. | to determine the frequency of mild/atypical herpes simplex virus encephalitis (hsve) among patients with csf specimens submitted to a university diagnostic virology laboratory for hsv pcr. | 1998 | 9710034 |
| effect of acridine derivatives on the multiplication of herpes simplex virus. | the antiviral effect of 22 acridine derivatives was studied on the multiplication of herpes simplex virus type 2 (hsv-2) in vero cells. the inhibitory effect of four acridine derivatives on the multiplication of hsv-2 is emphasised, six other compounds have moderate activity and three only slightly affect the virus multiplication, while nine compounds are inactive. some of the effective derivatives inhibit the virus replication without any direct effect on the virus particles. however, the mecha ... | 1998 | 9713501 |
| herpes simplex encephalitis and bilateral acute retinal necrosis syndrome after craniotomy. | acute retinal necrosis (arn) syndrome is associated with members of the herpes virus family, but the mechanisms of infection remain unclear. the purpose of this study is to report a unique case of acute retinal necrosis syndrome associated with herpetic encephalitis in order to elucidate possible factors involved in herpetic central nervous system disease. | 1998 | 9744385 |
| tumor necrosis factor-alpha response and herpesvirus infection in bell's palsy. | to attempt early diagnosis of patients with bell's palsy by detection of herpesviral dna in body fluids, and to investigate whether tumor necrosis factor-alpha (tnf-alpha), a cytokine associated with demyelination, is involved in the inflammatory response in this disease. | 1998 | 9707238 |
| characterization of the ul55 gene product of herpes simplex virus type 2. | we have identified the herpes simplex virus type 2 (hsv-2) ul55 gene product using a rabbit polyclonal antiserum raised against a recombinant 6 x his-ul55 fusion protein expressed in escherichia coli. the antiserum reacted specifically with a 23 kda protein in hsv-2 186-infected cell lysates. the protein was not detectable in the presence of the viral dna synthesis inhibitor phosphonoacetic acid. indirect immunofluorescence studies localized the ul55 protein within and at the periphery of the nu ... | 1998 | 9714248 |
| local periocular vaccination protects against eye disease more effectively than systemic vaccination following primary ocular herpes simplex virus infection in rabbits. | vaccination of experimental animals can provide efficient protection against ocular herpes simplex virus type 1 (hsv-1) challenge. although it is suspected that local immune responses are important in protection against ocular hsv-1 infection, no definitive studies have been done to determine if local ocular vaccination would produce more efficacious protection against hsv-1 ocular challenge than systemic vaccination. to address this question, we vaccinated groups of rabbits either systemically ... | 1998 | 9733807 |
| herpes simplex virus. | herpes simplex virus (hsv) infection is prevalent worldwide. herpes labialis, caused predominantly by hsv-1, and herpes vulvovaginitis, caused predominantly by hsv-2, may result in significant morbidity and mortality for infected neonates exposed during delivery. the diagnosis of hsv infection is made by serological testing, viral culture, or polymerase chain reaction. women with primary herpes vulvovaginitis exhibit a painful vesicular rash which is self-limited but may be followed by multiple ... | 1998 | 9738993 |
| mode of action of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl) cycloprop-1'-yl]methyl]guanine (a-5021) against herpes simplex virus type 1 and type 2 and varicella-zoster virus. | the mode of action of (1's,2'r)-9-([1', 2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl)guanine (a-5021) against herpes simplex virus type 1 (hsv-1), hsv-2, and varicella-zoster virus (vzv) was studied. a-5021 was monophosphorylated at the 2' site by viral thymidine kinases (tks). the 50% inhibitory values for thymidine phosphorylation of a-5021 by hsv-1 tk and hsv-2 tk were comparable to those for penciclovir (pcv) and lower than those for acyclovir (acv). of these three agents, a-5021 inhibited vz ... | 1998 | 9687413 |
| [significance of antibodies to herpesviridae viruses detectable in rheumatic diseases]. | to assay antibodies to cytomegalovirus (cmv), herpes simplex virus type 1 and 2 (hsv-1, hsv-2) and epstein-barr virus (ebv) in rheumatic patients and to clarify clinical correlations. | 1998 | 9644741 |
| characterization of the ul16 gene product of herpes simplex virus type 2. | we have raised rabbit polyclonal antisera against a his-tagged herpes simplex virus type 1 (hsv-1) ul16 fusion protein, one of which very specifically reacted with 40 kda and 41 kda proteins in the lysates of hsv-1 and hsv-2-infected cells, respectively. since its reactivity to the 41 kda protein was clearly eliminated by pre-adsorption with e. coli lysates expressing the ul16 fusion protein, the antiserum was used to characterize the ul16 products of hsv-2. the hsv-2 ul16 protein was produced a ... | 1998 | 9645194 |
| intranasal immunization of mice with herpes simplex virus type 2 recombinant gd2: the effect of adjuvants on mucosal and serum antibody responses. | mucosal immunization offers the potential for inducing iga antibody responses in the vagina, the site of infection for many viruses, including herpes simplex type 2 (hsv-2). to investigate this possibility, mice were immunized intranasally with 10 micrograms glycoprotein d2 (gd2) from hsv combined with a series of adjuvants of proven efficacy; the oil in water emulsion mf59, poly(d,l-lactide-co-glycolide) microparticles (plg) (encapsulated or co-administered), immune-stimulating complexes (iscom ... | 1998 | 9659230 |
| frequent recovery of hiv-1 from genital herpes simplex virus lesions in hiv-1-infected men. | genital ulcer disease has been epidemiologically linked as a risk factor in the transmission of the human immunodeficiency virus 1 (hiv-1). while herpes simplex virus 2 (hsv-2) is the most common cause of genital ulcers, no study has systematically evaluated the frequency or titer of hiv-1 virus in hsv-2 lesions. | 1998 | 9660365 |
| antiherpesvirus activities of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl]guanine (a-5021) in cell culture. | antiherpetic activity of (1's,2'r)-9-([1',2'-bis(hydroxymethyl)cycloprop-1'yl]methyl)guanine (a-5021) was compared with those of acyclovir (acv) and penciclovir (pcv) in cell cultures. in a plaque reduction assay using a selection of human cells, a-5021 showed the most potent activity in all cells. against clinical isolates of herpes simplex virus type 1 (hsv-1, n = 5) and type 2 (hsv-2, n = 6), mean 50% inhibitory concentrations (ic50s) for a-5021 were 0.013 and 0.15 microgram/ml, respectively, ... | 1998 | 9661001 |
| hiv-1 subtype e incidence and sexually transmitted diseases in a cohort of military conscripts in northern thailand. | to determine the rate of and risk factors for hiv-1 seroconversion and describe sexually transmitted disease (std) prevalence rates for young men in northern thailand. | 1998 | 9704943 |
| use of a neural network secondary structure prediction to define targets for mutagenesis of herpes simplex virus glycoprotein b. | herpes simplex virus glycoprotein b (hsv gb) is essential for penetration of virus into cells, for cell-to-cell spread of virus, and for cell-cell fusion. every member of the family herpesviridae has a gb homolog, underlining its importance. the antigenic structure of gb has been studied extensively, but little is known about which regions of the protein are important for its roles in virus entry and spread. in contrast to successes with other hsv glycoproteins, attempts to map functional domain ... | 1998 | 9712510 |
| a cell surface protein with herpesvirus entry activity (hveb) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus. | certain mutant strains of herpes simplex virus type 1 (hsv-1) are unable to infect cells in which entry is dependent on hvem, the previously described herpesvirus entry mediator designated here as herpesvirus entry protein a (hvea). these mutant viruses can infect other cells where entry is apparently dependent on other co-receptors. the mutant virus hsv-1(kos)rid1 was used to screen a human cdna expression library for ability of transfected plasmids to convert resistant chinese hamster ovary ce ... | 1998 | 9657005 |
| prevalence of papillomavirus, epstein-barr virus, cytomegalovirus, and herpes simplex virus type 2 in urinary bladder cancer. | recent epidemiological studies suggest that the risk for urological malignancies may be related to the exposure to infectious agents. human papillomaviruses type 16 and 18 (hpv 16, hpv 18), epstein-barr virus (ebv), cytomegalovirus (cmv) and herpes simplex virus type 2 (hsv-2) have been suggested previously as cofactors in the pathogenesis of some malignancies in humans. the present paper, the presence of hpv 16, hpv 18, ebv, cmv and hsv-2 genomes was investigated in a panel of 35 biopsies from ... | 1998 | 9661833 |
| t cell recognition motifs of an immunodominant peptide of myelin basic protein in patients with multiple sclerosis: structural requirements and clinical implications. | myelin basic protein (mbp)-reactive t cells may play an important role in the pathogenesis of multiple sclerosis (ms). the t cell response to the 83-99 region of mbp represents a dominant autoreactive response to mbp in ms patients of dr2 haplotype. in this study, a large panel of dr2- and dr4-restricted t cell clones specific for the mbp83-99 peptide were examined for the recognition motifs and structural requirements for antigen recognition using alanine-substituted peptides. our study reveale ... | 1998 | 9645371 |
| rapid detection, culture-amplification and typing of herpes simplex viruses by enzyme immunoassay in clinical samples. | the laboratory diagnosis of herpes simplex infection may require rapid (direct) tests, as well as cell cultures, for detection of the virus in clinical samples. the quantity of virus present in clinical samples is variable and this may depend on the period from onset of rash. in addition, not all patients may show obvious symptoms with this infection. the successful culture of herpes simplex virus requires prompt transportation after collection of the specimen as the virus is easily inactivated. ... | 1998 | 9646003 |
| cervical antibody responses to a herpes simplex virus type 2 glycoprotein subunit vaccine. | effective vaccines against genital herpes simplex virus type 2 (hsv-2) may need to induce genital tract immune responses. to determine local antibody responses to hsv-2 glycoproteins gb2 and gd2 in an intramuscular subunit vaccine, cervical secretions from hsv-seronegative women and hsv-1-seropositive women were tested for igg and iga to gb2 and gd2 by enhanced chemiluminescence western blot. most (94%) of the seronegative subjects developed cervical igg to gb2, igg to gd2, and iga to gb2; 72% d ... | 1998 | 9652416 |
| limited variability of glycoprotein gene sequences and neutralizing targets in herpes simplex virus type 2 isolates and stability on passage in cell culture. | nucleotide sequence analyses of polymerase chain reaction-amplified genes were performed to determine whether adaptation of herpes simplex virus type 2 to replication in cultured cells or in internal organs during neonatal disseminated disease results in selection of variants with altered forms of three glycoproteins (gb, gc, or gd) that influence virus entry into cells. no variations in sequence were noted as a consequence of in vitro passage or replication in different organs. five viruses fro ... | 1998 | 9652417 |
| synthesis and biological activity of 5-hydroxy-4-quinolones and 5-methoxy-4-quinolones as truncated acridones. | a series of 5-hydroxy-4-quinolone (3) and 5-methoxy-4-quinolone (4) derivatives were synthesized as truncated acridone analogues and evaluated for antitumor, antiherpes and antituberculosis activities. among them 5-hydroxy-8-methoxy-quinolone showed potent antitumor activity (ic50 = 17.7 microm for hl60) which was greater than that of acronycine. however, these compounds didn't show any significant antiherpes or antituberculosis activity. | 1998 | 9875474 |
| a new subtraction technique for molecular cloning of rare antiviral antibody specificities from phage display libraries. | the preparation of random combinatorial libraries exposed on the surface of phage provides a route for the selection of diverse high affinity human monoclonal antibodies. however, in particular settings, the isolation of genes coding for a rare antibody can be elusive because some epitopes are predominant and because, in the case of impure antigens, the protein or any compound of interest can be present in relatively minimal amount. in this paper, we describe the successful utilization of a new ... | 1998 | 9879613 |
| role of type specific herpes simplex virus serology in the diagnosis and management of genital herpes. | to investigate the indications for the use of a type specific antibody test for herpes simplex virus in a department of genitourinary medicine in the united kingdom. | 1998 | 9849551 |
| the anti-herpes simplex virus activity of n-docosanol includes inhibition of the viral entry process. | n-docosanol-treated cells resist infection by a variety of lipid-enveloped viruses including the herpesviruses. previous studies of the mechanism of action demonstrated that n-docosanol inhibits an event prior to the expression of intermediate early gene products but subsequent to hsv attachment. the studies reported here indicate that n-docosanol inhibits fusion of the hsv envelope with the plasma membrane. evidence suggests that antiviral activity requires a time-dependent metabolic conversion ... | 1998 | 9864049 |
| successful acyclovir treatment of herpes simplex type 2 hepatitis in a patient with systemic lupus erythematosus: a case report and meta analysis. | herpes simplex virus (hsv) hepatitis is a rare complication of hsv infection with a high reported mortality rate in untreated patients. the authors present a case of hsv hepatitis in a 26-year-old female with focal proliferative lupus nephropathy who was status post one cycle of pulse high-dose (1 gm/ m2) cyclophosphamide. treatment with parenteral acyclovir was successful. a meta analysis of well-documented cases of hsv hepatitis treated with acyclovir, excluding those that omit initial serum c ... | 1998 | 9856697 |