Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| protective immunity against hsv-2 in the mouse vagina. | herpes simplex virus type 2 (hsv-2) is a sexually transmitted pathogen that infects the genital tract. the high prevalence of hsv-2 in humans underscores the need to develop an effective vaccine. efforts to develop vaccines to protect women against this and other sexually transmitted pathogens would be facilitated by a better understanding of the immune mechanisms that protect the female reproductive tract against infections in animal models. such information would be invaluable in developing va ... | 1997 | 9430740 |
| herpes simplex virus type-2, cytomegalovirus and epstein-barr virus infection in acute non a to e hepatitis thai patients. | a significant number of acute non a to e hepatitis cases are reported in thailand every year, and the etiologies of these cases are unknown. members of the herpesviridae family have been reported to cause either a self limited or fatal hepatitis in a small proportion of patients in other parts of the world. to determine whether herpesviruses may play a role in acute non a to e hepatitis, sera from 32 acute hepatitis patients without markers for acute hepatitis a to e virus infection were examine ... | 1997 | 9438547 |
| evaluation of antioxidant healing formulations in topical therapy of experimental cutaneous and genital herpes simplex virus infections. | a genital hsv-2 infection of guinea pigs and a cutaneous hsv-1 infection in mice were used to examine the ability of antioxidant components crt1 to reduce lesion development, duration, and severity. crt is a patented antioxidant formulation developed by warner-lambert worldwide consumer healthcare r. and d. crt contains three components that work synergistically: vitamin e, sodium pyruvate and membrane stabilizing fatty acids. the ms strain of hsv-2 was utilized for intravaginal inoculation of g ... | 1997 | 9477116 |
| experimental myelitis caused by herpes simplex virus type 2 in c57bl/6n and balb/cn mice. | intraperitoneal and intracranial inoculation of herpes simplex virus type 2 (hsv 2) into balb/cn and c57bl/6n mice was carried out to induce experimental myelitis. the myelitis was clearly observed in c57bl/6n mice following intraperitoneal inoculation. within 24 hours before death, the mice showed urinary and rectal incontinence and paraplegia of the hind legs. randomly distributed, severe necrosis was demonstrated in the spinal cord, mainly at the lower cord. in balb/cn mice the clinical sympt ... | 1997 | 9516872 |
| t-lymphocytopaenia, opportunistic infections and pathological findings in ghanaian aids patients and their sexual partners. | ninety-nine patients at center for disease control (cdc) clinical stage iv were studied. twelve (12.12%) of these patients turned out to be hiv seronegative. ten out of the 12 hiv negative patients were immunocompetent whereas the other two had proportional decreases in both cd4+ and cd8+ t-lymphocytes. hiv-1, hiv-2, and dual infection, were detected in 51.5%, 2%, and 22.2% respectively of clinical aids patients. the other 12.12% of clinical aids patients were indeterminate for hiv antibodies. a ... | 1997 | 9557423 |
| dna increases the potency of vaccination against infectious diseases. | in the past couple of years, the idea that naked dna can be used to vaccinate against infections has been rapidly developing. in contrast to traditional protein or live attenuated vaccines, there is no risk of disease caused by dna vaccines as only selected proteins are encoded. the ease with which dna may be manipulated means that vaccines can be custom designed to meet many needs. in animal model systems, dna vaccines have proved to be as effective as traditional vaccines. additionally, this t ... | 1997 | 9667856 |
| genital herpes: epidemiology and pathophysiology. update and new perspectives. | genital herpes simplex virus (hsv) is a sexually transmitted disease (std) which affects millions of people worldwide and is mainly due to hsv type 2. seroprevalence rates as high as 60-90% have been reported in developing countries. in developed countries, 20% of the general population is hsv2 seropositive. recent epidemiological surveys employing type-specific antibody assays show that the prevalence of hsv-2 infections is rising at an alarming rate. also, the epidemiology is changing with an ... | 1997 | 9538399 |
| [molecular cloning and sequence analysis of glycoprotein d gene of herpes simplex virus 2 strain sav and wild strain isolated]. | to study the difference of structure and function of hsv-2 gd gene among the wild strain, g and sav strain. so as to provide theoretical basis for future production of hsv-2 vaccine. | 1997 | 9596937 |
| [monoclonal antibodies which recognize herpes simplex virus and their possible diagnostic application]. | it is reported the obtention of monoclonal antibodies, which are capable of recognizing hsv-1 and hsv-2 viruses, by the conventional technology of cellular fusion. the monoclonal antibodies of highest positivity according to the indirect immunofluorescence technique were partially characterized. it was determined the neutralizing capacity of 2 of them (278-f7 and 70-g10), which were used to identify isolations of clinical specimens by indirect immunofluorescence. these monoclonal antibodies reco ... | 1997 | 9685985 |
| prevalence of transfusion associated infections in multitransfused children in relation to mandatory screening of hiv in donated blood. | any change in risk behavior related to acquisition of human immunodeficiency virus (hiv) infection is likely to reduce simultaneously the risk for other agents transmitted through identical routes. a study carried out in the city of delhi, india on the load of transfusion associated infections among multitransfused (mt) children in relation to mandatory screening of hiv infection in donated blood indicated unchanged prevalence of hepatitis b virus (hbv), hepatitis c virus (hcv) and hepatitis d v ... | 1997 | 9656388 |
| tests of vaginal microbicides in the mouse genital herpes model. | microbicide candidates were selected that have demonstrated activity against sperm or sexually transmitted disease pathogens in vitro, and the efficacy of these agents for preventing vaginal transmission of genital herpes infection was evaluated in the progestin-treated mouse. each agent was delivered to the vaginas of mice approximately 20 sec prior to delivering a highly infectious herpes simplex virus-2 inoculum. the following agents provided significant protection: anti-hsv monoclonal antibo ... | 1997 | 9437563 |
| an in vitro assessment of the antineoplastic potential of 2h-1,3-oxazine-2,6(3h)-dione (3-oxauracil), a novel pyrimidine. | the pyrimidine (uracil) analogue 3-oxauracil (ou) previously had been shown to completely inhibit the growth of e. coli b and decrease by 96% the replication of herpes simplex virus type 2 when present in the culture fluid at a concentration of 10(2) microm. limited in vivo studies in mice demonstrated antiviral effects without significant toxicity when given i.p. daily for two weeks at a concentration of 3.23 mg/kg. however, the antineoplastic properties of ou were unknown. we assessed the abil ... | 1997 | 9547671 |
| [detecting pathogens of fetal defects in paraffin embedded tissues by polymerase chain reaction]. | to examine 3 types of pathogens in dead fetus with congenital defects for exploring the pathogenesis. | 1997 | 9639727 |
| a genetically inactivated herpes simplex virus type 2 (hsv-2) vaccine provides effective protection against primary and recurrent hsv-2 disease. | a glycoprotein h (gh)-deleted herpes simplex virus type 2 (hsv-2) was evaluated as a vaccine for the prevention of hsv-induced disease. this virus, which we term a disc (disabled infectious single cycle) virus, can only complete one replication cycle in normal cells and should thus be safe yet still able to stimulate broad humoral and cell-mediated antiviral immune responses. a gh-deleted hsv-2 virus that has been tested as a vaccine in the guinea pig model of recurrent hsv-2 infection was const ... | 1997 | 8985191 |
| specificity and affinity of binding of herpes simplex virus type 2 glycoprotein b to glycosaminoglycans. | herpes simplex virus type 2 (hsv-2) interacts with cell surface glycosaminoglycans during virus attachment. glycoprotein b of hsv-2 can potentially mediate the interaction between the virion and cell surface glycosaminoglycans. to determine the specificity, kinetics, and affinity of these interactions, we used plasmon resonance-based biosensor technology to measure hsv-2 glycoprotein binding to glycosaminoglycans in real time. the recombinant soluble ectodomain of hsv-2 gb (gb2) but not the solu ... | 1997 | 8995662 |
| [herpes simplex: generalized infection in a newborn]. | most mucose-cutaneous infections can be diagnosed by clinical history with explorative technique. nevertheless the definitive etiologic diagnostic can only be established with the help of the isolation and identification of the causal agent. | 1997 | 9101753 |
| [evaluation of the immune and interferon status of children infected with herpes simplex virus and living in areas with increased radiation background]. | virological and immunological examinations were carried out in 52 children aged 4 to 15 living at the bryansk district territories contaminated with radionuclides after the chernobyl accident. the persistence of herpes simplex virus (hsv) in the blood was detected by indirect immunofluorescence in 90% of children, in 10% it was confirmed by the virus isolation in vitro in a cell culture. specific antibodies to hsv-1 and hsv-2 were detected by enzyme immunoassay in 100% examinees, in 50% their ti ... | 1997 | 9103044 |
| evaluation of a quantitative competitive pcr assay for measuring herpes simplex virus dna content in genital tract secretions. | previous studies have shown an association between the approximate titer of herpes simplex virus (hsv) dna in clinical specimens and the ability to isolate hsv from genital secretions. to control for variance in amplification conditions, we developed a competitive quantitative pcr (qc pcr) for the detection of hsv dna. the assay accurately measured from 10 to 10(6) copies of hsv dna. we compared the qc pcr with our previous semiquantitative detection method and found concordance for 61 of 63 pos ... | 1997 | 9041386 |
| dextran sulfate can act as an artificial receptor to mediate a type-specific herpes simplex virus infection via glycoprotein b. | herpes simplex virus (hsv) adsorption to host cells is mediated, at least in part, by the interaction of viral glycoproteins with cell surface glycosaminoglycans such as heparan sulfate and chondroitin sulfate. to investigate the contribution of various cell surface components in the infection pathway, we isolated a mutant cell line, sog9, which is unable to synthesize glycosaminoglycans (b. w. banfield, y. leduc, l. esford, k. schubert, and f. tufaro, j. virol. 69:3290-3298, 1995). although hsv ... | 1997 | 8985338 |
| interferon-gamma enhances resolution of herpes simplex virus type 2 infection of the murine genital tract. | the requirement for interferon-gamma (ifn gamma) in resolution of an hsv-2 vaginal infection and the cellular sources of this cytokine in the vaginal mucosa were assessed. ifn gamma levels in vaginal secretions peaked on days 2 and 5 following hsv-2 inoculation. natural killer (nk) cell depletion greatly diminished the early production of ifn gamma but had no significant effect on the rate of virus clearance. cd4+ t cells were primarily responsible for the second peak of ifn gamma levels and neu ... | 1997 | 9123869 |
| treatment of spontaneously arising retinoblastoma tumors in transgenic mice with an attenuated herpes simplex virus mutant. | the use of viruses to treat tumors has received renewed interest with the availability of genetically defined attenuated mutants. herpes simplex virus (hsv) type 1 in particular has been shown to be effective for tumors of neuronal origin. however, the model systems used for these studies rely on the use of explanted tumor cells in immunodeficient animals. we have used a recently developed transgenic mouse model, wherein mice spontaneously develop retinoblastomas, to determine if a mutant hsv ha ... | 1997 | 9123872 |
| herpes simplex virus type 2 synergizes with interferon-gamma in the induction of nitric oxide production in mouse macrophages through autocrine secretion of tumour necrosis factor-alpha. | we have analysed the ability of herpes simplex virus type 2 (hsv-2) to induce nitric oxide (no) production in resting balb/c mouse peritoneal macrophages. in most experiments, macrophages produced very small amounts of no upon infection with hsv-2. mock virus preparations did not induce no production, and virus inactivation experiments showed that infectious virus was required. since interferon-gamma (ifn-gamma) is the prototype cytokine that is able to induce significant no production in macrop ... | 1997 | 9010304 |
| herpetic urethritis in male patients in ibadan. | in a study of 151 male patients attending a sexually transmitted disease clinic, 40 (26.4%) had non-gonococcal urethritis. herpes simplex virus was isolated in tissue culture of hep-2 cell line from 10 of the patients with non-gonococcal urethritis, and identified by complement fixation and indirect immunofluorescent anti body tests. of the isolates, 30% were herpes simplex virus type 1 (hsv-1), while 70% were herpes simplex virus types (hsv-2). | 1997 | 9133820 |
| pleural effusion due to herpes simplex type ii infection in an immunocompromised host. | pleural effusions caused by herpes simplex viruses are rare. we report a case of a young woman with acute lymphocytic leukemia (all) and prolonged neutropenia who developed pleural space infection with herpes simplex type ii virus (hsv ii), as confirmed by cytologic and microbiologic studies. we believe that this is the first report of a pleural effusion caused by hsv ii, and suggest that this virus now be considered in the differential diagnosis of an unexplained exudative pleural effusion, esp ... | 1997 | 9001338 |
| advantage of polymerase chain reaction in the diagnosis of herpes simplex encephalitis: presentation of 5 atypical cases. | four case of herpes encephalitis (hsve) are described. the diagnosis was established by polymerase chain reaction (pcr) assay of cerebrospinal fluid (csf). these reports illustrate different situations in the clinical management of this disease. pcr was considered useful in confirming the hsve diagnosis in 3 atypical cases, and in the differentiation between virologic failure and postinfectious encephalitis in a patient with recurrence of symptoms. a case with typical hsve clinical findings is a ... | 1997 | 9255880 |
| the acquisition of herpes simplex virus during pregnancy. | the acquisition of genital herpes during pregnancy has been associated with spontaneous abortion, prematurity, and congenital and neonatal herpes. the frequency of seroconversion, maternal symptoms of the disease, and the timing of its greatest effect on the outcome of pregnancy have not been systematically studied. | 1997 | 9262493 |
| definitions of genital ulcer disease and variation in risk for prevalent human immunodeficiency virus infection. | although genital ulcer disease (gud) has been associated with human immunodeficiency virus (hiv) infection in a number of studies, definitions of genital ulceration have varied. the authors hypothesized that the association of gud with prevalent hiv infection may vary according to the definition of gud that is used. | 1997 | 9263366 |
| [serous meningitis associated with primary genital herpes infection]. | aseptic meningitis is not an uncommon complication to primary genital herpes infection caused by herpes simplex virus type 2 (hsv-2). compared with other types of viral meningitis, hsv-2-meningitis is associated with a significant rate of neurological complications in the acute stage. in addition, some patients will suffer from recurrent aseptic meningitis (mollaret's meningitis) later. we describe six patients, five women and one man, age 26-35 years, with aseptic meningitis caused by hsv-2. al ... | 1997 | 9265274 |
| human papillomavirus type 16 and risk of preinvasive and invasive vulvar cancer: results from a seroepidemiological case-control study. | to examine whether human papillomavirus (hpv) type 16 is involved in the etiology of vulvar carcinomas. | 1997 | 9351758 |
| [mollaret meningitis. is the riddle finally solved?]. | mollaret's meningitis is characterised by recurrent aseptic meningitis in otherwise healthy persons. it has recently been shown that most cases are caused by herpes simplex virus type 2 (hsv-2). in this article we describe five women, age 33-57 years, with altogether 12 episodes of virus-culture negative aseptic meningitis, where polymerase chain reaction detected hsv-2 in samples of cerebrospinal fluid. only three patients had a medical history of previous genital herpes infection. none of our ... | 1997 | 9265275 |
| presence of herpes simplex dna in surgical tissue from human epileptic seizure foci detected by polymerase chain reaction: preliminary study. | to determine whether herpes simplex virus causes monofocal epilepsy and to assess the presence of herpes simplex virus 1 (hsv-1) and hsv-2 in surgical specimens from patients with epilepsy by using polymerase chain reaction and southern blot analysis. | 1997 | 9267969 |
| the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is associated with the virion tegument and has pk activity. | the large subunit of herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (icp10) was identified in sucrose gradient-purified hsv-2 virions by immunoprecipitation/immunoblotting with antibody specific for the protein kinase (pk) domain. immunoblotting of individual gradient fractions indicated that icp10 cosediments with the major capsid protein and the highest virus titers. icp10 was not labeled by iodination of purified virions, indicating that it is not located on the virion surface. ... | 1997 | 9268154 |
| active site cavity of herpesvirus proteases revealed by the crystal structure of herpes simplex virus protease/inhibitor complex. | human herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are responsible for herpes labialis (cold sores) and genital herpes, respectively. they encode a serine protease that is required for viral replication, and represent a viable target for therapeutic intervention. here, we report the crystal structures of hsv-1 and hsv-2 proteases, the latter in the presence and absence of the covalently bound transition state analog inhibitor diisopropyl phosphate (dip). the hsv-1 and hsv-2 protease st ... | 1997 | 9369473 |
| genital herpes infection: a review. | genital herpes infection is life-long and may result in painful and recurrent genital lesions, systemic complications, serious psychosocial morbidity, and rare but serious outcomes in neonates born to infected women, including permanent neurological handicap and death. herpes simplex virus (hsv)-2 is the principal cause, with an increasing proportion of first-episode disease caused by hsv-1. genital hsv transmission is usually due to asymptomatic viral shedding by people who are unaware that the ... | 1997 | 9279600 |
| herpes simplex virus seropositivity and reactivation at delivery among pregnant women infected with human immunodeficiency virus-1. | our purpose was to determine whether pregnant women infected with human immunodeficiency virus-1 have an increased risk of herpes simplex virus-2 seropositivity and herpes simplex virus reactivation at delivery. | 1997 | 9290467 |
| severe genital herpes infections in hiv-infected individuals with impaired herpes simplex virus-specific cd8+ cytotoxic t lymphocyte responses. | the specific mechanisms underlying the varied susceptibility of hiv-infected (hiv+) individuals to opportunistic infections (oi) are still incompletely understood. one hypothesis is that quantitative differences in specific t cell responses to a colonizing organism determine the development of an aids-defining oi. we evaluated this hypothesis for herpes simplex virus (hsv) infection, a common oi in hiv+ patients. using limiting dilution analyses, the frequency of hsv-specific cd8+ cytotoxic t ly ... | 1997 | 9294203 |
| hsv-1 and hsv-2 in herpes simplex encephalitis: a study of sixty-four cases in the united kingdom. | the incidence of herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) in herpes simplex encephalitis (hse) was investigated using cerebrospinal fluid (csf) samples from sixty-four cases of hse. a polymerase chain reaction (pcr) employing primers flanking a region of the hsv thymidine kinase gene common to both hsv-1 and hsv-2 was used to detect hsv in the csf. hsv-1 and hsv-2 were differentiated by digestion with restriction enzymes. two enzymes were employed; aval which c ... | 1997 | 9298724 |
| effect of herpes simplex virus on the dna of human papillomavirus 18. | some types of human papillomaviruses (hpv) appear to be associated with carcinoma of the cervix or other tissues, but patients infected with hpv do not necessarily develop carcinoma. some epidemiological studies of risk factors for cervical carcinoma have indicated the involvement of herpes simplex virus (hsv). to study the effect of hsv on the genome of hpv, total dnas were extracted and analyzed after hela cells, or a431 cells, transiently transfected with hpv18 dna, were infected with hsv-1 o ... | 1997 | 9298725 |
| in vitro inactivation of herpes simplex virus by a biological response modifier, psk. | herpes simplex virus (hsv) causes herpes genitalis, primary gingivostomatitis and recurrent herpes labialis. in order to elucidate in vivo mechanisms by which psk, a biological response modifier, exerts a protective effect against hsv infection, we used an in vitro system to study whether psk inactivated infectivity of hsv-type 1 (hsv-1) and hsv-type 2 (hsv-2) isolated from patients with herpes genitalis in addition to a laboratory-cultured strain of hsv type 1 (hsv-1-gc+). it was found that hsv ... | 1997 | 9298752 |
| sch 43478 and analogs: in vitro activity and in vivo efficacy of novel agents for herpesvirus type 2. | sch 43478 and analogs are a class of non-nucleoside antiviral agents that have potent and selective activity against herpes simplex virus type 2 (hsv-2). the ic50 for these compounds in plaque reduction analysis using vero cells ranges from 0.8 to 2.0 microg/ml. all compounds have a lc50 > 100 microg/ml in cytotoxicity analysis. mechanism of action studies suggest that these molecules have an effect on the transactivation of viral immediate early (alpha) gene expression. time of addition studies ... | 1997 | 9298753 |
| cutaneously applied acyclovir acts systemically in the treatment of herpetic infection in the hairless mouse. | using the skh-1 hairless mouse (hm) we have addressed the issue as to whether topically applied acyclovir (acv) may mediate some of its antiviral actions by a systemic effect. when topically applied in a formulation consisting of polyvinyl alcohol (25% w/v):dmso:cremophor el:linoleic acid (63:16:16:5, v/v/v/v), acv penetrated hairless mouse skin in a concentration-dependent manner and dose-dependently reduced cutaneous herpes simplex virus 1 (hsv-1) kos infection. topically applied acv also effe ... | 1997 | 9298755 |
| effect of il-4 and il-13 on ifn-gamma-induced production of nitric oxide in mouse macrophages infected with herpes simplex virus type 2. | interleukin (il)-4 and il-13 share a wide range of activities. prominent among these is the ability to antagonize many interferon (ifn)-gamma-induced activities. here we demonstrate that il-4 and il-13 totally abrogate ifn-gamma-induced nitric oxide (no) production and inducible nitric oxide synthase (inos) mrna and protein synthesis in a murine macrophage cell line. ifn-gamma-treated cells infected with herpes simplex virus type 2 (hsv-2) or costimulated with tumor necrosis factor (tnf)-alpha s ... | 1997 | 9305732 |
| mutational analysis of the virion host shutoff gene (ul41) of herpes simplex virus (hsv): characterization of hsv type 1 (hsv-1)/hsv-2 chimeras. | during lytic herpes simplex virus (hsv) infections, the half-lives of host and viral mrnas are regulated by the hsv virion host shutoff (vhs) protein (ul41). the sequences of the ul41 polypeptides of hsv type 1 (hsv-1) strain kos and hsv-2 strain 333 are 87% identical. in spite of this similarity, hsv-2 strains generally shut off the host more rapidly and completely than hsv-1 strains. to examine type-specific differences in vhs function, we compared the vhs activities of ul41 alleles from hsv-1 ... | 1997 | 9311788 |
| mutations in the 5' end of the herpes simplex virus type 2 latency-associated transcript (lat) promoter affect lat expression in vivo but not the rate of spontaneous reactivation of genital herpes. | the primary herpes simplex virus type 2 (hsv-2) latency-associated transcript (lat) promoter influences lat expression and rates of virus reactivation. we explored the biological importance of particular neuronally responsive regions within the promoter by creating new recombinant viruses bearing a targeted deletion (246 bp [strain 524]) or a point mutation (2 bp [strain 167]) in this region. these recombinant viruses grew efficiently in vitro and in vivo, caused acute genital disease in guinea ... | 1997 | 9311880 |
| prevalence of herpes simplex virus type 1- and 2- specific antibodies among the acute, recurrent, and provoked types of female genital herpes. | sixty-eight sera from the acute, recurrent, and provoked types of female genital herpes were compared for the seroprevalence of herpes simplex virus (hsv) types 1 and 2 by immunodot assay using hsv glycoprotein g. in the hsv-1-isolated patients, no hsv-2 antibodies were detected, whereas in the hsv-2-isolated patients, hsv-1 seroprevalence was 9% for the acute type, 89% for the provoked type (p < 0.005), and 55% for the recurrent type (p < 0.05). the natural history of female genital herpes and ... | 1997 | 9403510 |
| genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins b and d. | intramuscular injection of mice with an adeno-associated virus (aav) vector expressing herpes simplex virus type 2 glycoprotein b led to the generation of both gb-specific major histocompatibility complex class i-restricted cytotoxic t lymphocytes and anti-gb antibody. aav-mediated immunization was more potent than plasmid dna or protein in generating antibody responses. | 1997 | 9311887 |
| mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from bacillus subtilis. | the antiviral activity of surfactin, a cyclic lipopeptide antibiotic and biosurfactant produced by bacillus subtilis, was determined for a broad spectrum of different viruses, semliki forest virus (sfv), herpes simplex virus (hsv-1, hsv-2), suid herpes virus (shv-1), vesicular stomatitis virus (vsv), simian immunodeficiency virus (siv), feline calicivirus (fcv), murine encephalomyocarditis virus (emcv). in vitro experiments showed biphasic virus inactivation kinetics for enveloped viruses during ... | 1997 | 9324997 |
| herpes simplex virus type 2 in the united states, 1976 to 1994. | herpes simplex virus type 2 (hsv-2) infection is usually transmitted sexually and can cause recurrent, painful genital ulcers. in neonates the infection is potentially lethal. we investigated the seroprevalence and correlates of hsv-2 infection in the united states and identified changes in hsv-2 seroprevalence since the late 1970s. | 1997 | 9329932 |
| herpes simplex virus type 2--a persistent problem. | 1997 | 9329938 | |
| immunoglobulin g is the main protective antibody in mouse vaginal secretions after vaginal immunization with attenuated herpes simplex virus type 2. | we investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (hsv-2). unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin g (igg) and secretory iga (s-iga) from immune secretions were adjusted to their concentrations in vivo. wild-type hsv-2 was incubated in the immunoglobulin preparations for 15 min in vitro, followed by inoculation into vaginae of non ... | 1997 | 9343160 |
| herpes simplex virus type 2 infection in a 5-year-old boy presenting with recurrent chest wall vesicles and a possible history of herpes encephalitis. | a 5-year-old hyperkinetic but otherwise healthy child presented with recurrent irritable vesicles and erosions of the anterior chest wall; they have been apparent since the age of 15 months. wound swab cultures yielded herpes simplex virus type-2 (hsv-2) and western blot serology showed past exposure to both hsv-1 and hsv-2. skin biopsy results further supported a herpes virus infection. magnetic resonance imaging of the brain showed right temporal lobe atrophy. an evaluation showed no evidence ... | 1997 | 9349346 |
| structure-activity relationships of 2'-deoxy-2',2'-difluoro-l-erythro-pentofuranosyl nucleosides. | following the recent discoveries that some l-nucleosides are more or equal potent than their d-counterparts, we synthesized 2'-deoxy-2',2'-difluoro-l-erythro-pentofuranosyl nucleosides as potential antiviral agents. the target compounds were synthesized via the key intermediates 7a or 7b from l-gulono gamma-lactone. compound 2 was oxidatively cleaved and coupled with ethyl bromodifluoroacetate in the presence of activated zinc under reformatsky conditions to obtain a diasteomeric mixture of 4(r) ... | 1997 | 9357530 |
| immunotherapy of recurrent genital herpes with recombinant herpes simplex virus type 2 glycoproteins d and b: results of a placebo-controlled vaccine trial. | to determine the safety, immunogenicity, and efficacy of a recombinant herpes simplex virus type 2 glycoprotein d and b vaccine in the treatment of recurrent genital herpes, a randomized, placebo-controlled trial was held at two referral centers. healthy patients with 4-14 recurrences per year received injections of both glycoproteins in mf59 adjuvant or of mf59 alone at 0, 2, 12, and 14 months. for 18 study months, the rate and number of recurrences, the duration and severity of the first confi ... | 1997 | 9359709 |
| tyrosine phosphorylation of the herpes simplex virus type 1 regulatory protein icp22 and a cellular protein which shares antigenic determinants with icp22. | at least eight herpes simplex virus type 1 (hsv-1) and five hsv-2 proteins were tyrosine phosphorylated in infected cells. the first viral tyrosine phosphoprotein identified was the hsv-1 regulatory protein icp22. also, two novel phosphotyrosine proteins were bound by anti-icp22 antibodies. h(r22) is a cellular protein, while the f(r10) protein is observed only in hsv-1-infected cells. | 1997 | 9371655 |
| [central nervous system infection due to herpes simplex virus in aids]. | infections of the central nervous system by herpes simplex viruses (herpes simplex type 1 and herpes simplex type 2) are uncommon in acquired immune deficiency syndrome and are often clinically and pathologically atypical. we have collected 11 cases of herpes simplex encephalomyelitis in aids patients reported in the literature. only 3 of these cases presented with a typical, necrotizing, limbic encephalitis. other clinicopathological patterns included ventriculitis, rhombencephalitis and myelit ... | 1997 | 9382607 |
| acceptability of genital herpes immunization. the role of health beliefs and health behaviors. | little is known about the acceptability of vaccines for prevention of sexually transmitted diseases (stds). the purpose of this study was to examine potential predictors of genital herpes simplex virus type 2 (hsv-2) vaccine acceptability. | 1997 | 9383842 |
| quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of epstein-barr virus burden in posttransplant lymphoproliferative disorder. | human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. we report the development and validation of a quantitative pcr method to measure viral burden for all eight human herpesviruses (hsv1, hsv2, vzv, ebv, cmv, hhv6, hhv7, and kshv) in patients' samples. the method uses an internal standard that is coamplified with the ... | 1997 | 9342002 |
| herpes simplex virus. | cns infections caused by human herpesvirus 1 and human herpesvirus 2-herpes simplex virus type 1 and herpes simplex virus type 2--are reviewed. the major diseases associated with these viruses are meningitis and encephalitis. two forms of encephalitis are known, neonatal encephalitis and encephalitis occurring after the neonatal period. both diseases are associated with high mortality and morbidity and require prompt diagnosis and aggressive antiviral chemotherapy. methods for the specific diagn ... | 1997 | 9450224 |
| herpes simplex vegetans: atypical genital herpes infection in a patient with common variable immunodeficiency. | large papillomatous lesions clinically resembling verrucous carcinoma may be caused by viruses other than human papillomavirus. we report a case of recurrent vegetations covering the entire vulva in a pregnant patient with common variable immunodeficiency. herpes simplex virus was recovered from these lesions. the patient did not respond to intravenous acyclovir, but her lesions dramatically healed with two courses of intravenous foscarnet. repeated biopsies may prove necessary in cases such as ... | 1997 | 9366853 |
| cytomegalovirus infection and atherosclerosis. | the biological properties of human cytomegalovirus are consistent with pathogenic involvement at several levels of the atherogenic process. although the sites of latency of cmv have not been established, both smooth muscle cells and leukocytes are likely possibilities. the observations of viral antigens and nucleic acid sequences in arterial smooth muscle cells suggest that latent cmv infection of the arterial wall may be common in patients with atherosclerosis. the seroepidemiologic studies sug ... | 1997 | 9386894 |
| hsv type specific antibody tests: patients are ready, are clinicians? | 1997 | 9389940 | |
| patient attitudes to type specific serological tests in the diagnosis of genital herpes. | to assess patient attitudes to hsv-2 serotesting and the effect of providing detailed information regarding genital herpes, the blood test, and its implications. | 1997 | 9389945 |
| a novel method to assay herpes simplex virus neutralizing antibodies using bhkicp6lacz-5 (elvis) cells. | a novel method for determining neutralizing serum antibody titers to herpes simplex virus type 2 (hsv-2) was developed based on reduction of infectivity in bhkicp6lacz-5 (elvis) cells; baby hamster kidney (bhk) cells that have been genetically engineered to contain the escherichia coli lacz gene under the control of an inducible herpes simplex virus type 1 (hsv-1) promoter. the test has a semiautomated, colorimetric readout resulting in rapid, objective readings of infectivity reduction. extent ... | 1997 | 9473152 |
| coagulation initiated on herpesviruses. | herpesviruses have been previously correlated to vascular disease and shown to cause thrombogenic and atherogenic changes to host cells. herein we show that even in the absence of cells, purified cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) can initiate thrombin production. functional assays demonstrated that purified hsv-1 and hsv-2 provide the necessary phospholipid (propl) for assembling the coagulation factors xa and va into prothrombinase, which is respon ... | 1997 | 9391056 |
| a sero-epidemiological study of the relationship between sexually transmitted agents and cervical cancer in honduras. | to investigate a possible cause-and-effect relationship between sexually transmitted diseases and cervical cancer, we performed a sero-epidemiological study on the presence of antibodies against a number of sexually transmitted agents (stas) in patients with cervical cancer and their matched controls. in this study, we used serological techniques to investigate the presence of antibodies to cytomegalovirus, herpes simplex virus type 2, human immunodeficiency virus, chlamydia trachomatis, trepone ... | 1997 | 9399651 |
| an open-label study conducted to evaluate the efficacy of betadine cold sore paint. | an open-label, randomised, parallel-group efficacy study was designed to compare betadine cold sore paint and stoxil topical ointment for the prevention of shedding of herpes simplex virus (hsv) from cold sores. seventy-two patients aged 18-61 years (mean 32.2 years), with symptoms indicating recent onset of herpes labialis, were entered into the study. patients were randomised to receive betadine cold sore paint, stoxil topical ointment or no treatment. to detect infectious virus, swabs were ta ... | 1997 | 9403262 |
| antiviral activity of natural sulphated galactans on herpes virus multiplication in cell culture. | a sulphated galactan (sg) with low molecular weight (app. 2800) was isolated from extracts of cryptopleura ramosa, a red seaweed from the south american coasts. the compound was a selective inhibitor of hsv-1 and hsv-2 replication in vero cells with 50% inhibitory concentrations (ic50) in the range 1.6-4.2 micrograms/ml and a 50% cytotoxic concentration (cc50) of 476 micrograms/ml. sg was also effective against hsv-1 in cells of neural origin such as murine astrocytes. the mode of action of sg c ... | 1997 | 9342947 |
| analysis of cyclin-dependent kinase activity after herpes simplex virus type 2 infection. | small dna viruses (adenoviruses, simian virus 40, or human papillomaviruses) induce s-phase progression but prevent cell division to provide precursors for viral dna replication. herpes simplex viruses types 1 or 2 (hsv-1 or hsv-2) contain genes which encode dna-metabolizing enzymes, for example, ribonucleotide reductase, thymidine kinase and dutpase, suggesting that s-phase factors are not required for an efficient infection. however, several studies indicated that hsv induces some events that ... | 1997 | 9400986 |
| [an autopsied case doubly infected by mucor mycelia and herpes simplex virus-2 in the remission state after bone marrow transplantation for acute myelogenous leukemia]. | a 36-year-old woman who had been in complete remission after bone marrow transplantation against her leukemia presented with visual disturbance and cerebellar signs. she showed unusual involuntary movement as if she rolled a ball of string, and died two months after the onset of illness. necropsy revealed hemorrhagic and necrotic lesions in the thalamus, basal ganglia, cerebellar dentate nucleus, superior colliculus, midbrain pretectum and red nucleus, and mucor mycelia were numerously found. hs ... | 1997 | 9163758 |
| ap-1 cis-response elements are involved in basal expression and vmw110 transactivation of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the promoter of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) has two ap-1 cis-response elements, respectively located at positions -62 and -94 relative to the transcription start site (wymer et al., 1989. j. virol. 63, 2773-2784). chloramphenicol acetyl transferase (cat) analysis with hybrid constructions of the cat structural gene and the icp10 promoter or its mutants and gel retardation studies were used to examine the role of the ap-1 cis-response elements ... | 1997 | 9168892 |
| genital herpes during pregnancy: inability to distinguish primary and recurrent infections clinically. | to determine if the signs and symptoms of genital herpes in pregnancy accurately identify primary genital herpes infections using serologic testing for final classification. | 1997 | 9170460 |
| herpes simplex virus virulence: the functions of the gamma (1)34.5 gene. | 1997 | 9179780 | |
| antiherpetic and anticoagulant properties of carrageenans from the red seaweed gigartina skottsbergii and their cyclized derivatives: correlation between structure and biological activity. | the antiviral activity against herpes simplex virus types 1 and 2 of kappa/l-, partially cyclized mu/v-, and lambda-carrageenans isolated from the red seaweed gigartina skottsbergii and their cyclized derivatives was analyzed. lambda-carrageenans and the partially cyclized mu/v-carrageenan were the most potent inhibitors of herpes viruses (including acyclovir-resistant variants and clinical isolates), with ic50 values lower than 1 microgram ml-1 against both serotypes and selectivity indices hig ... | 1997 | 9184941 |
| inhibition of human immunodeficiency virus replication by the herpes simplex virus virion host shutoff protein. | the herpes simplex virus (hsv) virion host shutoff gene (vhs) encodes a protein which nonspecifically accelerates the degradation of mrna molecules, leading to inhibition of protein synthesis. this ability to inhibit a critical cellular function suggested that vhs could be used as a suicide gene in certain gene therapy applications. to investigate whether vhs might be useful for treatment of aids, we tested the ability of both hsv type 1 (hsv-1) and hsv-2 vhs to inhibit replication of human immu ... | 1997 | 9188626 |
| herpes virus-specific immune responses in individuals experiencing recurrent genital herpes. | 1997 | 9191322 | |
| herpes simplex virus-specific igm, iga and igg subclass antibody responses in primary and nonprimary genital herpes patients. | to clarify the humoral immunity in herpes simplex virus (hsv) infection, hsv-specific igm, iga and igg subclass antibody responses were studied in patients with genital herpes: 17 primary, 13 recurrent and 6 nonprimary first episode. a total of 181 serum samples serially collected from the patients, 5 per patient until 213 days after the onset of disease (on average), were analyzed by an enzyme-linked immunosorbent assay. igg1, igg3 and iga were detected in all patients with primary and nonprima ... | 1997 | 9194040 |
| fine-resolution analysis of products of intrachromosomal homeologous recombination in mammalian cells. | mouse ltk- cell lines that contained a herpes simplex virus type 1 (hsv-1) thymidine kinase (tk) gene with a 16-bp insertion mutation linked to either a defective hsv-2 tk gene or a hybrid tk sequence comprised of hsv-1 and hsv-2 tk sequences were constructed. hsv-1 and hsv-2 tk genes have 81% nucleotide identity and hence are homeologous. correction of the insertion mutant hsv-1 tk gene via recombination with the hybrid tk sequence required an exchange between homeologous tk sequences, although ... | 1997 | 9199296 |
| monoclonal antibodies to the distinct antigenic sites on glycoproteins c and b and their protective abilities in herpes simplex virus infection. | the relative importance of the humoral immune response to various antigenic sites on the glycoproteins c and b (gc, gb) of herpes simplex virus (hsv) was evaluated in balb/c and dba/2 mice passively immunized with monoclonal antibodies (moabs) and then challenged with lethal dose of infectious virus. eight moabs to three topographically distinct antigenic sites on gc and eight moabs to two distinct antigenic sites on gb were selected. the results indicated that any antigenic site on gc and gb co ... | 1997 | 9199708 |
| antiviral and tumor cell antiproliferative sar studies on tetracyclic eudistomins. ii. | in a search for the minimum pharmacophore of the naturally occurring tetracyclic eudistomins, five structural analogues (4-8) were evaluated for their in vitro antiviral and tumor cell antiproliferative activities. for the synthesis of these derivatives both intra- and intermolecular pictet-spengler reactions have been used. opening of the beta-carboline annulated 7-membered d-ring in 6 and 7 resulted in a complete loss of activity. on the other hand, replacement of either the oxygen atom or the ... | 1997 | 9208105 |
| apoptosis induced in the spinal cord and dorsal root ganglion by infection of herpes simplex virus type 2 in the mouse. | after inoculation of a highly neuro-invasive strain of herpes simplex virus (hsv) type 2 (186) into the mouse hind-paw planter skin, many virus-positive neurons and glial cells were detected in the dorsal root ganglia (drgs) and lumbar spinal cord by immunohistochemistry for hsv-2 antigen. a number of apoptotic cells were also observed in the spinal cord and drgs by the terminal dutp nick-end-labeling (tunel) method. double labeling with the immunohistochemistry for hsv-2 and the tunel method re ... | 1997 | 9209108 |
| a system for isolation, transport and storage of herpes simplex viruses. | a major difficulty with diagnostic virus isolation concerns the relative thermolability of certain viruses, e.g. herpes simplex virus type 2, which may, therefore, lose infectivity during transport to the laboratory. this study describes a system of virus isolation and transport, which depends on direct inoculation at the bedside or clinic, to a monolayer or suspension of susceptible cells with subsequent incubation for 10 h at approximately 32 degrees c, whereupon the newly synthesised virus be ... | 1997 | 9128856 |
| preferential presentation of herpes simplex virus t-cell antigen by hla dqa1*0501/dqb1*0201 in comparison to hla dqa1*0201/dqb1*0201. | the hla dqa1 locus is polymorphic. haplotypes containing hla dqa1*0501, but not hla dqa1*0201, together with hla dqb1*0201 are associated with grave's disease and celiac sprue. in this report, we demonstrate a functional correlate of dqa1 polymorphism. t cells infiltrating a herpes simplex virus (hsv) lesion from a hla dq 2,7 individual yielded a virus-specific cd4+ clone restricted by dq2. presentation of viral peptide and protein segregated with dqa1 allele, because cell lines bearing dqa1*050 ... | 1997 | 9129979 |
| genital herpes in a primary care clinic. demographic and sexual correlates of herpes simplex type 2 infections. | genital herpes remains one of the most prevalent sexually transmitted diseases (stds). the sexual behavioral correlates of herpes simplex virus type 2 (hsv-2) infection in the general population have not been well characterized. | 1997 | 9132981 |
| differential t cell response induced by certain recombinant oligopeptides of herpes simplex virus glycoprotein b in mice. | much attention is presently focused on the quality of the immune response produced by helper t or regulatory cells because of its implications for vaccine development and immunomodulation. glycoprotein b (gb) of herpes simplex virus (hsv) has been shown to induce a protective t cell response. to further characterize the nature of the t cell response, oligopeptides were expressed from the open reading frame of gb from hsv-2 (gb-2) as fusion proteins with beta-galactosidase (gz) in e. coli. after ... | 1997 | 9225039 |
| clear detection and typing of herpes simplex virus types 1 and 2 by an indirect elisa assay: comparison with three different combined methods--capture elisa, restriction enzymes, and polymerase chain reaction. | the severity and recurrences of herpes simplex virus (hsv) infection depend on the type of the infectious agent (hsv-1 or hsv-2), which induces the necessity of a nonambiguous detecting typing. the commonly used capture elisa technique has to be often supported by dna analysis to confirm the detection and the typing of hsv viruses in exposed patients. in this report, we describe a rapid and cheap indirect elisa method using anti-hsv monospecific polyclonal antibodies prepared in the laboratory. ... | 1997 | 9138103 |
| herpes simplex blepharoconjunctivitis presenting as complete acquired ankyloblepharon. | a 90-year-old woman was referred to us with a 1-month history of progressively worsening blepharoconjunctivitis. she had a complete acquired ankyloblepharon of the right lids, which resulted in the appearance of a right upper lid abscess on computed tomography. | 1997 | 9143812 |
| sexually-transmitted disease (std) markers in multitransfused children in relation to human immunodeficiency virus type-1 (hiv-1) infection: impact of std markers in blood donors. | association of serological markers for various sexually transmitted diseases (stds) with human immunodeficiency virus type 1 (hiv-1) infection among heterosexually promiscuous blood donors is well recognized. however, possibility of similar association among children receiving transfusion from these donors has not been adequately explored. study on the association of some std markers with hiv-1 infection in multitransfused (mt) children showed that both hiv-1 positive and hiv-1 negative groups o ... | 1997 | 9231641 |
| localization of latency-associated transcripts in the uterovaginal plexus of herpes simplex virus type 1 and 2 latently infected mice. | the vagina and medulla of the adrenal gland of mice vaginally infected with herpes simplex virus (hsv) types 1 and 2 were examined in the latent stage of infection (5 to 51 weeks post-infection). rna in situ hybridization with hsv-1 and -2 latency-associated transcript (lat) rna probes resulted in positively stained neuronal cell nuclei in the uterovaginal plexus, but not in the medulla of the adrenal gland. these organs were chosen because hsv antigens can be detected not only in the vaginal ep ... | 1997 | 9152429 |
| the prophylactic effect of immunization with dna encoding herpes simplex virus glycoproteins on hsv-induced disease in guinea pigs. | plasmid expression vectors encoding herpes simplex virus type 2 (hsv-2) glycoproteins b (gb) or d (gd) were constructed and tested for their ability to immunize guinea pigs against genital hsv infection. immunization with a plasmid expressing the aminoterminal 707 amino acids (aa) of gb induced humoral immune responses detected by elisa and virus neutralization. when challenged by vaginal infection, immunized animals were partially protected from genital herpes, exhibiting significantly reduced ... | 1997 | 9234533 |
| sex, drugs, and infections among youth. parenterally and sexually transmitted diseases in a high-risk neighborhood. | to determine the extent to which youth who reside in households in a neighborhood with large numbers of drug injectors 1) are infected with parenterally or sexually transmitted agents, and 2) engage in high-risk behaviors. | 1997 | 9243738 |
| evaluation of a non-isotopic polymerase chain reaction assay for detection in clinical specimens of herpes simplex virus type 2 dna. canadian women's hiv study group. | pcr assays for detection of herpes simplex virus dna sequences in clinical specimens are more sensitive than cell culture. | 1997 | 9248658 |
| detection and differentiation of primate alpha-herpesviruses by pcr. | a rapid method for detection and differentiation of 5 primate alpha-herpesviruses (human herpes simplex virus types 1 and 2 [hsv1, hsv2], green monkey simian agent 8, baboon herpesvirus 2 [hvp2], and macaque b virus [bv]) was developed utilizing the polymerase chain reaction (pcr). pcr primers were located in conserved regions of the gene encoding the glycoprotein b, which flanks an intervening region that is highly divergent among the 5 viruses. amplified pcr products from the 5 viruses were re ... | 1997 | 9249159 |
| antibody to haemophilus ducreyi among trucking company workers in kenya. | to determine the prevalence, correlates, and incidence of haemophilus ducreyi antibodies, a cohort of east african trucking company employees was evaluated. | 1997 | 9153735 |
| european consensus on viral encephalitis. | 1997 | 9024370 | |
| the active site of icp47, a herpes simplex virus-encoded inhibitor of the major histocompatibility complex (mhc)-encoded peptide transporter associated with antigen processing (tap), maps to the nh2-terminal 35 residues. | the herpes simplex virus (hsv) immediate early protein icp47 inhibits the transporter associated with antigen processing (tap)-dependent peptide translocation. as a consequence, empty major histocompatibility complex (mhc) class i molecules are retained in the endoplasmic reticulum and recognition of hsv-infected cells by cytotoxic t lymphocytes is abolished. we chemically synthesized full-length icp47 (sicp47) and show that sicp47 inhibits tap-dependent peptide translocation in human cells. its ... | 1997 | 9151894 |
| mechanism of complement inactivation by glycoprotein c of herpes simplex virus. | glycoprotein c (gc) of both herpes simplex virus type 1 (hsv-1) and hsv-2 interacts with complement c3b and protects the virus from complement-mediated neutralization. to study the mechanism by which gc modulates complement activation, we expressed both gc-1 and gc-2 in a baculovirus expression system. baculovirus recombinants containing gc genes spanning the entire gc-1 sequence (gc-1-tmr) or only the extracellular domain(s) of gc-1, gc-2, or a deletion mutant of gc-1 lacking residues 33 throug ... | 1997 | 9029114 |
| viral lesions of the mouth in hiv-infected patients. | viral lesions of the mouth in patients with hiv infection are common and these diseases any be a marker for hiv and disease progression. we review the spectrum of oral viral manifestations and discuss treatment modalities. the most common epstein-barr virus (ebv)-induced disorder in hiv-infected patients is oral hairy leukoplakia. ebv-related oral b-cell and t-cell lymphoma in aids patients has been described repeatedly. herpes virus type 1 and rarely type 2 may lead to painful and resistant ora ... | 1997 | 9031782 |
| asymptomatic perianal shedding of herpes simplex virus in patients with acquired immunodeficiency syndrome. | to determine the frequency of asymptomatic perianal shedding of herpes simplex virus (hsv) in adult patients with acquired immunodeficiency syndrome (aids). | 1997 | 9041831 |
| novel 3'-c/n-substituted 2',3'-beta-d-dideoxynucleosides as potential chemotherapeutic agents. 1. thymidine derivatives: synthesis, structure, and broad spectrum antiviral properties. | a synthetic scheme for the 3'-oxime derivatives 3e, 5e, 5z, 7e and 7z of 1-(2,3-dideoxy-beta-d-glycero-pentofuranosyl)thymine and for 1-(2,3-dideoxy-3-nitro-beta-d-erythro-pentofuranosyl)-thymine (10) has been developed starting from appropriately 5'-protected 3'-ketothymidine. x-ray analysis showed that 3'-n-hydroxyimino 3e and 3'-n-methoxyimino 5z derivatives have close molecular conformations: anti about the n1-c1' bond, and gauche+ about the c4'-c5' exocyclic bond. their sugar conformations ... | 1997 | 9046339 |
| detection and typing of herpes simplex viruses by using recombinant immunoglobulin fragments produced in bacteria. | thirty-seven bacterial clones producing human recombinant monoclonal antibody fab fragments (rfabs) reactive to herpes simplex virus (hsv) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an hsv lysate. thirty-four of the hsv-specific rfabs were able to specifically recognize hsv-infected cells in indirect immunofluorescence (if) assays; of these, 25 recognized cells infected by either hsv type 1 (hsv-1) or hs ... | 1997 | 9163470 |