Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| vaginal formulations of carrageenan protect mice from herpes simplex virus infection. | the observations from the present study indicate that vaginal formulations of the sulfated polysaccharide carrageenan are highly effective in protecting mice from herpes simplex virus type 2 (hsv-2) infection. test formulations were placed in the vaginas of progestin-treated mice prior to inoculation with hsv-2. infection was determined by the presence of inflammation in the genital region and death. at a dose of virus that infected half of the control animals, 1% solutions of either lambda, kap ... | 1997 | 9220165 |
| neuronal sprouting in mouse sensory ganglia infected with herpes simplex virus type 2 (hsv-2): induction of growth-associated protein (gap-43) and ultrastructural evidence. | herpes simplex virus (hsv) is neurotropic and when inoculated on the mouse footpad is retrogradely transported to the associated dorsal root ganglia (drg), where infection is established. previous observations suggest that, after hsv infection, sensory ganglion neurons may mount a sprouting response. in our hsv-infected drg model, we investigate this issue by (1) examining expression of growth-associated protein (gap-43), a molecule known to be induced by growing axons, and (2) determining ultra ... | 1995 | 9222354 |
| characterization of a bhk(tk-) cell clone resistant to postattachment entry by herpes simplex virus types 1 and 2. | bhk(tk-) cells selected for resistance to polyethylene glycol-mediated fusion give rise to clones that are resistant to herpes simplex virus (hsv) infection. we have characterized one such clone, designated 95-19, and found that it is resistant to entry of hsv type 1 (hsv-1), hsv-2, and the related alphaherpesvirus pseudorabies virus (prv). single-step growth experiments show no detectable replication of multiple strains of hsv-1 and hsv-2 on 95-19 cells. three lines of evidence suggest that the ... | 1997 | 9223469 |
| selection of a nonconsensus branch point is influenced by an rna stem-loop structure and is important to confer stability to the herpes simplex virus 2-kilobase latency-associated transcript. | herpes simplex virus type 1 latent infection in sensory neurons is characterized by the highly restricted transcription of viral genes. the latency-associated transcripts (lat) family members are the only transcripts that can be identified in large amounts in latently infected cells. the most abundant lat species is a 2-kb rna that results from splicing of a rare primary transcript. analysis of a lat mutant virus (tb1) in cell culture revealed an aberrant splicing pattern and production of a sta ... | 1997 | 9223474 |
| low prevalences of hiv infection and sexually transmitted disease among female commercial sex workers in mexico city. | this study tried to determine human immunodeficiency virus (hiv)/sexually transmitted disease (std) prevalences among female commercial sex workers in mexico city. | 1997 | 9224186 |
| differential t cell response induced by certain recombinant oligopeptides of herpes simplex virus glycoprotein b in mice. | much attention is presently focused on the quality of the immune response produced by helper t or regulatory cells because of its implications for vaccine development and immunomodulation. glycoprotein b (gb) of herpes simplex virus (hsv) has been shown to induce a protective t cell response. to further characterize the nature of the t cell response, oligopeptides were expressed from the open reading frame of gb from hsv-2 (gb-2) as fusion proteins with beta-galactosidase (gz) in e. coli. after ... | 1997 | 9225039 |
| [seroprevalence of various viruses and sociodemographic characteristics of women seeking hiv screening]. | to study the seroprevalence of syphilis, herpes simplex-2, hepatitis b and hiv, and main sociodemographic characteristics and sexual behavior in women seeking testing for human immunodeficiency virus (hiv) in mexico city. | 1997 | 9229756 |
| hand trauma and herpes infection. | a herpes simplex type 2 infection of the hand after injury is described in a 26-year-old man. the management and implications of such an unusual hand infection are discussed. | 1996 | 9230963 |
| sexually-transmitted disease (std) markers in multitransfused children in relation to human immunodeficiency virus type-1 (hiv-1) infection: impact of std markers in blood donors. | association of serological markers for various sexually transmitted diseases (stds) with human immunodeficiency virus type 1 (hiv-1) infection among heterosexually promiscuous blood donors is well recognized. however, possibility of similar association among children receiving transfusion from these donors has not been adequately explored. study on the association of some std markers with hiv-1 infection in multitransfused (mt) children showed that both hiv-1 positive and hiv-1 negative groups o ... | 1997 | 9231641 |
| viral regulation of cd95 expression and apoptosis in t lymphocytes. | t lymphocyte apoptosis has been observed in vivo in association with viral infections. one mechanism known to mediate t cell apoptosis is the cd95 (fas/apo-1) ag apoptotic pathway. cd95 is a cell surface protein that is expressed on activated t cells and is capable of relaying an intracellular apoptotic signal upon binding with cd95 ligand. cd95-dependent apoptosis has been shown to be involved in the homeostatic control of peripheral t cell numbers; however, the functional significance of the c ... | 1997 | 9233613 |
| the prophylactic effect of immunization with dna encoding herpes simplex virus glycoproteins on hsv-induced disease in guinea pigs. | plasmid expression vectors encoding herpes simplex virus type 2 (hsv-2) glycoproteins b (gb) or d (gd) were constructed and tested for their ability to immunize guinea pigs against genital hsv infection. immunization with a plasmid expressing the aminoterminal 707 amino acids (aa) of gb induced humoral immune responses detected by elisa and virus neutralization. when challenged by vaginal infection, immunized animals were partially protected from genital herpes, exhibiting significantly reduced ... | 1997 | 9234533 |
| sex, drugs, and infections among youth. parenterally and sexually transmitted diseases in a high-risk neighborhood. | to determine the extent to which youth who reside in households in a neighborhood with large numbers of drug injectors 1) are infected with parenterally or sexually transmitted agents, and 2) engage in high-risk behaviors. | 1997 | 9243738 |
| evaluation of a non-isotopic polymerase chain reaction assay for detection in clinical specimens of herpes simplex virus type 2 dna. canadian women's hiv study group. | pcr assays for detection of herpes simplex virus dna sequences in clinical specimens are more sensitive than cell culture. | 1997 | 9248658 |
| detection and differentiation of primate alpha-herpesviruses by pcr. | a rapid method for detection and differentiation of 5 primate alpha-herpesviruses (human herpes simplex virus types 1 and 2 [hsv1, hsv2], green monkey simian agent 8, baboon herpesvirus 2 [hvp2], and macaque b virus [bv]) was developed utilizing the polymerase chain reaction (pcr). pcr primers were located in conserved regions of the gene encoding the glycoprotein b, which flanks an intervening region that is highly divergent among the 5 viruses. amplified pcr products from the 5 viruses were re ... | 1997 | 9249159 |
| etiology of acute non-a, b, c hepatitis in thai patients: preliminary study. | to better characterize the etiology of acute non-a, b, c hepatitis, 24 sera from 50 acute hepatitis without acute markers for hepatitis a, b, and c were examined for acute markers for the hepatitis e virus (hev), cytomegalovirus (cmv), herpes simplex virus type 2 (hsv-2), and epstein-barr virus. immunoglobulin m (igm) specific for hev, hsv-2, and cmv was detected using elisa and total ig specific to ebv was determined by standard indirect immunofluorescence. igm to cmv was not observed in sera f ... | 1996 | 9253895 |
| advantage of polymerase chain reaction in the diagnosis of herpes simplex encephalitis: presentation of 5 atypical cases. | four case of herpes encephalitis (hsve) are described. the diagnosis was established by polymerase chain reaction (pcr) assay of cerebrospinal fluid (csf). these reports illustrate different situations in the clinical management of this disease. pcr was considered useful in confirming the hsve diagnosis in 3 atypical cases, and in the differentiation between virologic failure and postinfectious encephalitis in a patient with recurrence of symptoms. a case with typical hsve clinical findings is a ... | 1997 | 9255880 |
| the acquisition of herpes simplex virus during pregnancy. | the acquisition of genital herpes during pregnancy has been associated with spontaneous abortion, prematurity, and congenital and neonatal herpes. the frequency of seroconversion, maternal symptoms of the disease, and the timing of its greatest effect on the outcome of pregnancy have not been systematically studied. | 1997 | 9262493 |
| definitions of genital ulcer disease and variation in risk for prevalent human immunodeficiency virus infection. | although genital ulcer disease (gud) has been associated with human immunodeficiency virus (hiv) infection in a number of studies, definitions of genital ulceration have varied. the authors hypothesized that the association of gud with prevalent hiv infection may vary according to the definition of gud that is used. | 1997 | 9263366 |
| [serous meningitis associated with primary genital herpes infection]. | aseptic meningitis is not an uncommon complication to primary genital herpes infection caused by herpes simplex virus type 2 (hsv-2). compared with other types of viral meningitis, hsv-2-meningitis is associated with a significant rate of neurological complications in the acute stage. in addition, some patients will suffer from recurrent aseptic meningitis (mollaret's meningitis) later. we describe six patients, five women and one man, age 26-35 years, with aseptic meningitis caused by hsv-2. al ... | 1997 | 9265274 |
| [mollaret meningitis. is the riddle finally solved?]. | mollaret's meningitis is characterised by recurrent aseptic meningitis in otherwise healthy persons. it has recently been shown that most cases are caused by herpes simplex virus type 2 (hsv-2). in this article we describe five women, age 33-57 years, with altogether 12 episodes of virus-culture negative aseptic meningitis, where polymerase chain reaction detected hsv-2 in samples of cerebrospinal fluid. only three patients had a medical history of previous genital herpes infection. none of our ... | 1997 | 9265275 |
| presence of herpes simplex dna in surgical tissue from human epileptic seizure foci detected by polymerase chain reaction: preliminary study. | to determine whether herpes simplex virus causes monofocal epilepsy and to assess the presence of herpes simplex virus 1 (hsv-1) and hsv-2 in surgical specimens from patients with epilepsy by using polymerase chain reaction and southern blot analysis. | 1997 | 9267969 |
| the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is associated with the virion tegument and has pk activity. | the large subunit of herpes simplex virus type 2 (hsv-2) ribonucleotide reductase (icp10) was identified in sucrose gradient-purified hsv-2 virions by immunoprecipitation/immunoblotting with antibody specific for the protein kinase (pk) domain. immunoblotting of individual gradient fractions indicated that icp10 cosediments with the major capsid protein and the highest virus titers. icp10 was not labeled by iodination of purified virions, indicating that it is not located on the virion surface. ... | 1997 | 9268154 |
| genital herpes infection: a review. | genital herpes infection is life-long and may result in painful and recurrent genital lesions, systemic complications, serious psychosocial morbidity, and rare but serious outcomes in neonates born to infected women, including permanent neurological handicap and death. herpes simplex virus (hsv)-2 is the principal cause, with an increasing proportion of first-episode disease caused by hsv-1. genital hsv transmission is usually due to asymptomatic viral shedding by people who are unaware that the ... | 1997 | 9279600 |
| herpes simplex virus seropositivity and reactivation at delivery among pregnant women infected with human immunodeficiency virus-1. | our purpose was to determine whether pregnant women infected with human immunodeficiency virus-1 have an increased risk of herpes simplex virus-2 seropositivity and herpes simplex virus reactivation at delivery. | 1997 | 9290467 |
| severe genital herpes infections in hiv-infected individuals with impaired herpes simplex virus-specific cd8+ cytotoxic t lymphocyte responses. | the specific mechanisms underlying the varied susceptibility of hiv-infected (hiv+) individuals to opportunistic infections (oi) are still incompletely understood. one hypothesis is that quantitative differences in specific t cell responses to a colonizing organism determine the development of an aids-defining oi. we evaluated this hypothesis for herpes simplex virus (hsv) infection, a common oi in hiv+ patients. using limiting dilution analyses, the frequency of hsv-specific cd8+ cytotoxic t ly ... | 1997 | 9294203 |
| hsv-1 and hsv-2 in herpes simplex encephalitis: a study of sixty-four cases in the united kingdom. | the incidence of herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) in herpes simplex encephalitis (hse) was investigated using cerebrospinal fluid (csf) samples from sixty-four cases of hse. a polymerase chain reaction (pcr) employing primers flanking a region of the hsv thymidine kinase gene common to both hsv-1 and hsv-2 was used to detect hsv in the csf. hsv-1 and hsv-2 were differentiated by digestion with restriction enzymes. two enzymes were employed; aval which c ... | 1997 | 9298724 |
| effect of herpes simplex virus on the dna of human papillomavirus 18. | some types of human papillomaviruses (hpv) appear to be associated with carcinoma of the cervix or other tissues, but patients infected with hpv do not necessarily develop carcinoma. some epidemiological studies of risk factors for cervical carcinoma have indicated the involvement of herpes simplex virus (hsv). to study the effect of hsv on the genome of hpv, total dnas were extracted and analyzed after hela cells, or a431 cells, transiently transfected with hpv18 dna, were infected with hsv-1 o ... | 1997 | 9298725 |
| in vitro inactivation of herpes simplex virus by a biological response modifier, psk. | herpes simplex virus (hsv) causes herpes genitalis, primary gingivostomatitis and recurrent herpes labialis. in order to elucidate in vivo mechanisms by which psk, a biological response modifier, exerts a protective effect against hsv infection, we used an in vitro system to study whether psk inactivated infectivity of hsv-type 1 (hsv-1) and hsv-type 2 (hsv-2) isolated from patients with herpes genitalis in addition to a laboratory-cultured strain of hsv type 1 (hsv-1-gc+). it was found that hsv ... | 1997 | 9298752 |
| sch 43478 and analogs: in vitro activity and in vivo efficacy of novel agents for herpesvirus type 2. | sch 43478 and analogs are a class of non-nucleoside antiviral agents that have potent and selective activity against herpes simplex virus type 2 (hsv-2). the ic50 for these compounds in plaque reduction analysis using vero cells ranges from 0.8 to 2.0 microg/ml. all compounds have a lc50 > 100 microg/ml in cytotoxicity analysis. mechanism of action studies suggest that these molecules have an effect on the transactivation of viral immediate early (alpha) gene expression. time of addition studies ... | 1997 | 9298753 |
| cutaneously applied acyclovir acts systemically in the treatment of herpetic infection in the hairless mouse. | using the skh-1 hairless mouse (hm) we have addressed the issue as to whether topically applied acyclovir (acv) may mediate some of its antiviral actions by a systemic effect. when topically applied in a formulation consisting of polyvinyl alcohol (25% w/v):dmso:cremophor el:linoleic acid (63:16:16:5, v/v/v/v), acv penetrated hairless mouse skin in a concentration-dependent manner and dose-dependently reduced cutaneous herpes simplex virus 1 (hsv-1) kos infection. topically applied acv also effe ... | 1997 | 9298755 |
| purification and characterization of the protein kinase encoded by the ul13 gene of herpes simplex virus type 2. | the proteins encoded by the ul13 genes of herpes simplex virus types 1 (hsv-1) and 2 (hsv-2) have been predicted to be protein kinases. to identify the ul13 gene product, we have raised a rabbit polyclonal antiserum against a his.tag-hsv-1 ul13 fusion protein. the antibody specifically reacted with the 60-kda ul13 fusion protein expressed in escherichia coli and also recognized 56- to 57-kda late proteins in nuclear fractions of hsv-1- and hsv-2-infected cells. on the other hand, novel casein ki ... | 1997 | 9300039 |
| effect of il-4 and il-13 on ifn-gamma-induced production of nitric oxide in mouse macrophages infected with herpes simplex virus type 2. | interleukin (il)-4 and il-13 share a wide range of activities. prominent among these is the ability to antagonize many interferon (ifn)-gamma-induced activities. here we demonstrate that il-4 and il-13 totally abrogate ifn-gamma-induced nitric oxide (no) production and inducible nitric oxide synthase (inos) mrna and protein synthesis in a murine macrophage cell line. ifn-gamma-treated cells infected with herpes simplex virus type 2 (hsv-2) or costimulated with tumor necrosis factor (tnf)-alpha s ... | 1997 | 9305732 |
| mutational analysis of the virion host shutoff gene (ul41) of herpes simplex virus (hsv): characterization of hsv type 1 (hsv-1)/hsv-2 chimeras. | during lytic herpes simplex virus (hsv) infections, the half-lives of host and viral mrnas are regulated by the hsv virion host shutoff (vhs) protein (ul41). the sequences of the ul41 polypeptides of hsv type 1 (hsv-1) strain kos and hsv-2 strain 333 are 87% identical. in spite of this similarity, hsv-2 strains generally shut off the host more rapidly and completely than hsv-1 strains. to examine type-specific differences in vhs function, we compared the vhs activities of ul41 alleles from hsv-1 ... | 1997 | 9311788 |
| mutations in the 5' end of the herpes simplex virus type 2 latency-associated transcript (lat) promoter affect lat expression in vivo but not the rate of spontaneous reactivation of genital herpes. | the primary herpes simplex virus type 2 (hsv-2) latency-associated transcript (lat) promoter influences lat expression and rates of virus reactivation. we explored the biological importance of particular neuronally responsive regions within the promoter by creating new recombinant viruses bearing a targeted deletion (246 bp [strain 524]) or a point mutation (2 bp [strain 167]) in this region. these recombinant viruses grew efficiently in vitro and in vivo, caused acute genital disease in guinea ... | 1997 | 9311880 |
| genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins b and d. | intramuscular injection of mice with an adeno-associated virus (aav) vector expressing herpes simplex virus type 2 glycoprotein b led to the generation of both gb-specific major histocompatibility complex class i-restricted cytotoxic t lymphocytes and anti-gb antibody. aav-mediated immunization was more potent than plasmid dna or protein in generating antibody responses. | 1997 | 9311887 |
| mechanism of inactivation of enveloped viruses by the biosurfactant surfactin from bacillus subtilis. | the antiviral activity of surfactin, a cyclic lipopeptide antibiotic and biosurfactant produced by bacillus subtilis, was determined for a broad spectrum of different viruses, semliki forest virus (sfv), herpes simplex virus (hsv-1, hsv-2), suid herpes virus (shv-1), vesicular stomatitis virus (vsv), simian immunodeficiency virus (siv), feline calicivirus (fcv), murine encephalomyocarditis virus (emcv). in vitro experiments showed biphasic virus inactivation kinetics for enveloped viruses during ... | 1997 | 9324997 |
| the active domain of the herpes simplex virus protein icp47: a potent inhibitor of the transporter associated with antigen processing. | the herpes simplex virus type 1 (hsv-1) protein icp47 binds specifically to the transporter associated with antigen processing (tap), thereby blocking peptide-binding and translocation by tap and subsequent loading of peptides onto mhc class i molecules in the endoplasmic reticulum. in consequence, hsv-infected cells are masked for immune recognition by cytotoxic t-lymphocytes. to investigate the molecular details of this, so far, unique transporter-inhibitor interaction, the active domain and c ... | 1997 | 9325106 |
| challenges for vaccination against sexually-transmitted diseases: induction and long-term maintenance of mucosal immune responses in the female genital tract. | the ability to develop vaccines capable of preventing or protecting against sexually transmitted viruses, such as herpes simplex virus (hsv) and hiv is likely to depend on the induction of long-term mucosal immune responses. we have utilized a recombinant adenovirus capable of expressing hsv glycoprotein b (adgb8) to demonstrate that intranasal (i.n.) immunization induces hsvgb-specific igg and iga antibodies in the serum and vaginal washes of mice. in contrast, systemic immunization only result ... | 1997 | 9327525 |
| herpes simplex virus type 2 in the united states, 1976 to 1994. | herpes simplex virus type 2 (hsv-2) infection is usually transmitted sexually and can cause recurrent, painful genital ulcers. in neonates the infection is potentially lethal. we investigated the seroprevalence and correlates of hsv-2 infection in the united states and identified changes in hsv-2 seroprevalence since the late 1970s. | 1997 | 9329932 |
| herpes simplex virus type 2--a persistent problem. | 1997 | 9329938 | |
| antiviral properties of 3-quinolinecarboxamides: a series of novel non-nucleoside antiherpetic agents. | novel antiherpetic 3-quinolinecarboxamides were discovered as part of a drug discovery program at sterling winthrop inc. a major goal of this research was to identify novel non-nucleoside agents possessing activity against acyclovir resistant herpes simplex virus. from screening compound libraries in an hsv-2 plaque reduction assay, 1-ethyl-1,4-dihydro-4-oxo-7-(4-pyridinyl)-3-quinolinecarboxamide (1) emerged as an attractive lead structure. by modifying the quinoline ring at the 1-, 2-, 3-, 4-, ... | 1997 | 9332829 |
| cofactors with human papillomavirus in a population-based study of vulvar cancer. | human papillomavirus (hpv) has been previously associated with vulvar cancer. in a population-based study, we examined whether exposure to hpv, cigarette smoking, or herpes simplex virus 2 (hsv2) increases the risk of this cancer. | 1997 | 9337348 |
| quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of epstein-barr virus burden in posttransplant lymphoproliferative disorder. | human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. we report the development and validation of a quantitative pcr method to measure viral burden for all eight human herpesviruses (hsv1, hsv2, vzv, ebv, cmv, hhv6, hhv7, and kshv) in patients' samples. the method uses an internal standard that is coamplified with the ... | 1997 | 9342002 |
| antiviral activity of natural sulphated galactans on herpes virus multiplication in cell culture. | a sulphated galactan (sg) with low molecular weight (app. 2800) was isolated from extracts of cryptopleura ramosa, a red seaweed from the south american coasts. the compound was a selective inhibitor of hsv-1 and hsv-2 replication in vero cells with 50% inhibitory concentrations (ic50) in the range 1.6-4.2 micrograms/ml and a 50% cytotoxic concentration (cc50) of 476 micrograms/ml. sg was also effective against hsv-1 in cells of neural origin such as murine astrocytes. the mode of action of sg c ... | 1997 | 9342947 |
| immunoglobulin g is the main protective antibody in mouse vaginal secretions after vaginal immunization with attenuated herpes simplex virus type 2. | we investigated the protective role of antibodies in vaginal secretions of mice that were immune to vaginal challenge with herpes simplex virus type 2 (hsv-2). unfractionated vaginal immunoglobulins from immune and nonimmune mice and affinity-purified immunoglobulin g (igg) and secretory iga (s-iga) from immune secretions were adjusted to their concentrations in vivo. wild-type hsv-2 was incubated in the immunoglobulin preparations for 15 min in vitro, followed by inoculation into vaginae of non ... | 1997 | 9343160 |
| herpes simplex virus type 2 infection in a 5-year-old boy presenting with recurrent chest wall vesicles and a possible history of herpes encephalitis. | a 5-year-old hyperkinetic but otherwise healthy child presented with recurrent irritable vesicles and erosions of the anterior chest wall; they have been apparent since the age of 15 months. wound swab cultures yielded herpes simplex virus type-2 (hsv-2) and western blot serology showed past exposure to both hsv-1 and hsv-2. skin biopsy results further supported a herpes virus infection. magnetic resonance imaging of the brain showed right temporal lobe atrophy. an evaluation showed no evidence ... | 1997 | 9349346 |
| human papillomavirus type 16 and risk of preinvasive and invasive vulvar cancer: results from a seroepidemiological case-control study. | to examine whether human papillomavirus (hpv) type 16 is involved in the etiology of vulvar carcinomas. | 1997 | 9351758 |
| structure-activity relationships of 2'-deoxy-2',2'-difluoro-l-erythro-pentofuranosyl nucleosides. | following the recent discoveries that some l-nucleosides are more or equal potent than their d-counterparts, we synthesized 2'-deoxy-2',2'-difluoro-l-erythro-pentofuranosyl nucleosides as potential antiviral agents. the target compounds were synthesized via the key intermediates 7a or 7b from l-gulono gamma-lactone. compound 2 was oxidatively cleaved and coupled with ethyl bromodifluoroacetate in the presence of activated zinc under reformatsky conditions to obtain a diasteomeric mixture of 4(r) ... | 1997 | 9357530 |
| immunotherapy of recurrent genital herpes with recombinant herpes simplex virus type 2 glycoproteins d and b: results of a placebo-controlled vaccine trial. | to determine the safety, immunogenicity, and efficacy of a recombinant herpes simplex virus type 2 glycoprotein d and b vaccine in the treatment of recurrent genital herpes, a randomized, placebo-controlled trial was held at two referral centers. healthy patients with 4-14 recurrences per year received injections of both glycoproteins in mf59 adjuvant or of mf59 alone at 0, 2, 12, and 14 months. for 18 study months, the rate and number of recurrences, the duration and severity of the first confi ... | 1997 | 9359709 |
| herpes simplex vegetans: atypical genital herpes infection in a patient with common variable immunodeficiency. | large papillomatous lesions clinically resembling verrucous carcinoma may be caused by viruses other than human papillomavirus. we report a case of recurrent vegetations covering the entire vulva in a pregnant patient with common variable immunodeficiency. herpes simplex virus was recovered from these lesions. the patient did not respond to intravenous acyclovir, but her lesions dramatically healed with two courses of intravenous foscarnet. repeated biopsies may prove necessary in cases such as ... | 1997 | 9366853 |
| active site cavity of herpesvirus proteases revealed by the crystal structure of herpes simplex virus protease/inhibitor complex. | human herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) are responsible for herpes labialis (cold sores) and genital herpes, respectively. they encode a serine protease that is required for viral replication, and represent a viable target for therapeutic intervention. here, we report the crystal structures of hsv-1 and hsv-2 proteases, the latter in the presence and absence of the covalently bound transition state analog inhibitor diisopropyl phosphate (dip). the hsv-1 and hsv-2 protease st ... | 1997 | 9369473 |
| tyrosine phosphorylation of the herpes simplex virus type 1 regulatory protein icp22 and a cellular protein which shares antigenic determinants with icp22. | at least eight herpes simplex virus type 1 (hsv-1) and five hsv-2 proteins were tyrosine phosphorylated in infected cells. the first viral tyrosine phosphoprotein identified was the hsv-1 regulatory protein icp22. also, two novel phosphotyrosine proteins were bound by anti-icp22 antibodies. h(r22) is a cellular protein, while the f(r10) protein is observed only in hsv-1-infected cells. | 1997 | 9371655 |
| [central nervous system infection due to herpes simplex virus in aids]. | infections of the central nervous system by herpes simplex viruses (herpes simplex type 1 and herpes simplex type 2) are uncommon in acquired immune deficiency syndrome and are often clinically and pathologically atypical. we have collected 11 cases of herpes simplex encephalomyelitis in aids patients reported in the literature. only 3 of these cases presented with a typical, necrotizing, limbic encephalitis. other clinicopathological patterns included ventriculitis, rhombencephalitis and myelit ... | 1997 | 9382607 |
| acceptability of genital herpes immunization. the role of health beliefs and health behaviors. | little is known about the acceptability of vaccines for prevention of sexually transmitted diseases (stds). the purpose of this study was to examine potential predictors of genital herpes simplex virus type 2 (hsv-2) vaccine acceptability. | 1997 | 9383842 |
| cytomegalovirus infection and atherosclerosis. | the biological properties of human cytomegalovirus are consistent with pathogenic involvement at several levels of the atherogenic process. although the sites of latency of cmv have not been established, both smooth muscle cells and leukocytes are likely possibilities. the observations of viral antigens and nucleic acid sequences in arterial smooth muscle cells suggest that latent cmv infection of the arterial wall may be common in patients with atherosclerosis. the seroepidemiologic studies sug ... | 1997 | 9386894 |
| hsv type specific antibody tests: patients are ready, are clinicians? | 1997 | 9389940 | |
| patient attitudes to type specific serological tests in the diagnosis of genital herpes. | to assess patient attitudes to hsv-2 serotesting and the effect of providing detailed information regarding genital herpes, the blood test, and its implications. | 1997 | 9389945 |
| coagulation initiated on herpesviruses. | herpesviruses have been previously correlated to vascular disease and shown to cause thrombogenic and atherogenic changes to host cells. herein we show that even in the absence of cells, purified cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) can initiate thrombin production. functional assays demonstrated that purified hsv-1 and hsv-2 provide the necessary phospholipid (propl) for assembling the coagulation factors xa and va into prothrombinase, which is respon ... | 1997 | 9391056 |
| a sero-epidemiological study of the relationship between sexually transmitted agents and cervical cancer in honduras. | to investigate a possible cause-and-effect relationship between sexually transmitted diseases and cervical cancer, we performed a sero-epidemiological study on the presence of antibodies against a number of sexually transmitted agents (stas) in patients with cervical cancer and their matched controls. in this study, we used serological techniques to investigate the presence of antibodies to cytomegalovirus, herpes simplex virus type 2, human immunodeficiency virus, chlamydia trachomatis, trepone ... | 1997 | 9399651 |
| analysis of cyclin-dependent kinase activity after herpes simplex virus type 2 infection. | small dna viruses (adenoviruses, simian virus 40, or human papillomaviruses) induce s-phase progression but prevent cell division to provide precursors for viral dna replication. herpes simplex viruses types 1 or 2 (hsv-1 or hsv-2) contain genes which encode dna-metabolizing enzymes, for example, ribonucleotide reductase, thymidine kinase and dutpase, suggesting that s-phase factors are not required for an efficient infection. however, several studies indicated that hsv induces some events that ... | 1997 | 9400986 |
| the association of herpes simplex virus type 2 (hsv-2), haemophilus ducreyi, and syphilis with hiv infection in young men in northern thailand. | to evaluate the association between sexually transmitted diseases that commonly may cause genital ulceration and prevalent and incident hiv infections, we conducted three case control studies in a cohort of 21-year-old male military conscripts in northern thailand. the men were evaluated at baseline in 1991 and semiannually until their discharge 2 years later. serologic evidence of infection with herpes simplex virus type 2 (hsv-2), haemophilus ducreyi, and hiv were more frequent at baseline in ... | 1997 | 9402077 |
| an open-label study conducted to evaluate the efficacy of betadine cold sore paint. | an open-label, randomised, parallel-group efficacy study was designed to compare betadine cold sore paint and stoxil topical ointment for the prevention of shedding of herpes simplex virus (hsv) from cold sores. seventy-two patients aged 18-61 years (mean 32.2 years), with symptoms indicating recent onset of herpes labialis, were entered into the study. patients were randomised to receive betadine cold sore paint, stoxil topical ointment or no treatment. to detect infectious virus, swabs were ta ... | 1997 | 9403262 |
| prevalence of herpes simplex virus type 1- and 2- specific antibodies among the acute, recurrent, and provoked types of female genital herpes. | sixty-eight sera from the acute, recurrent, and provoked types of female genital herpes were compared for the seroprevalence of herpes simplex virus (hsv) types 1 and 2 by immunodot assay using hsv glycoprotein g. in the hsv-1-isolated patients, no hsv-2 antibodies were detected, whereas in the hsv-2-isolated patients, hsv-1 seroprevalence was 9% for the acute type, 89% for the provoked type (p < 0.005), and 55% for the recurrent type (p < 0.05). the natural history of female genital herpes and ... | 1997 | 9403510 |
| detection of herpes simplex virus type-specific antibodies by an enzyme-linked immunosorbent assay based on glycoprotein g. | in order to develop a simple and quantitative method to detect herpes simplex virus (hsv) type-specific antibodies, the usefulness of an enzyme-linked immunosorbent assay (elisa) using hsv glycoprotein g (gg) captured on a plate by monoclonal antibodies as antigen was studied. the gg1- and gg2-specific igg antibody activities were measured by the elisa for 54 sera which had been collected from culture-proven genital herpes patients and pre-characterized by an immunodot assay using purified gg an ... | 1997 | 9407378 |
| oral shedding of herpes simplex virus type 1: a review. | herpes simplex virus type 1 (hsv-1) and, to a lesser extent, type 2 (hsv-2) are the aetiological agents of recrudescent herpes labialis (rhl). the available literature on patterns of hsv-1 shedding into the oral cavity at the prodromal stage of disease, during recrudescences and also during asymptomatic periods, is reviewed, as are the potential sources of virus and the known trigger factors leading to viral reactivation. attention is given to the methodologies in use for the detection of hsv-1 ... | 1997 | 9416573 |
| the gh-gl complex of herpes simplex virus (hsv) stimulates neutralizing antibody and protects mice against hsv type 1 challenge. | the herpes simplex virus type 1 (hsv-1) gh-gl complex which is found in the virion envelope is essential for virus infectivity and is a major antigen for the host immune system. however, little is known about the precise role of gh-gl in virus entry, and attempts to demonstrate the immunologic or vaccine efficacy of gh and gl separately or as the gh-gl complex have not succeeded. we constructed a recombinant mammalian cell line (hl-7) which secretes a soluble gh-gl complex, consisting of gh trun ... | 1998 | 9420201 |
| herpes simplex virus type 1 renders infected cells resistant to cytotoxic t-lymphocyte-induced apoptosis. | many viruses interfere with apoptosis of infected cells, presumably preventing cellular apoptosis as a direct response to viral infection. since cytotoxic t lymphocytes (ctl) induce apoptosis of infected cells as part of the "lethal hit," inhibition of apoptosis could represent an effective immune evasion strategy. we report here herpes simplex virus type 1 (hsv-1) interference with ctl-induced apoptosis of infected cells and show that hsv-1 inhibits the nuclear manifestations of apoptosis but n ... | 1998 | 9420243 |
| congenital infections caused by varicella zoster virus and herpes simplex virus. | congenital infections are caused by both varicella-zoster virus (vzv) and herpes simplex virus type 2 (hsv-2). the vzv fetopathy and the hsv fetopathy exhibit several similarities. both affect the skin, eyes and brain, often with devastating consequences. congenital vzv infection also may damage portions of the cervical or lumbosacral plexi, an insult that leads to denervation and maldevelopment of an extremity. as is evident from the pathology, most of the fetal sequelae caused by intrauterine ... | 1994 | 9422218 |
| multinucleated epithelial giant cell changes in esophagitis: a clinicopathologic study of 14 cases. | we describe the clinical and pathologic features of a hitherto unreported finding in patients with esophagitis: the presence of multinucleated squamous epithelial giant cells simulating viral cytopathic effect and/or dysplasia. routinely processed hematoxylin and eosin (h&e)-stained slides of esophageal mucosal biopsies from 14 patients with both active esophagitis and multinucleated epithelial giant cells were evaluated for a variety of inflammatory and epithelial features. clinical, endoscopic ... | 1998 | 9422321 |
| famciclovir for the suppression of symptomatic and asymptomatic herpes simplex virus reactivation in hiv-infected persons. a double-blind, placebo-controlled trial. | herpes simplex virus (hsv) infection is one of the most common opportunistic infections in hiv-infected persons. however, most documentation of the effectiveness of antiviral therapy in reducing hsv reactivation is anecdotal. | 1998 | 9424977 |
| the r1 subunit of herpes simplex virus ribonucleotide reductase is a good substrate for host cell protein kinases but is not itself a protein kinase. | the n terminus of the r1 subunit of herpes simplex virus type 2 ribonucleotide reductase is believed to be a protein kinase domain mainly because the r1 protein was phosphorylated in a protein kinase assay on blot. using escherichia coli and adenovirus expression vectors to produce r1, we found that, whereas the reductase activity of both recombinant proteins was similar, efficient phosphorylation of r1 and casein in the presence of mg2+ was obtained only with the r1 purified from eukaryotic cel ... | 1998 | 9430680 |
| protective immunity against hsv-2 in the mouse vagina. | herpes simplex virus type 2 (hsv-2) is a sexually transmitted pathogen that infects the genital tract. the high prevalence of hsv-2 in humans underscores the need to develop an effective vaccine. efforts to develop vaccines to protect women against this and other sexually transmitted pathogens would be facilitated by a better understanding of the immune mechanisms that protect the female reproductive tract against infections in animal models. such information would be invaluable in developing va ... | 1997 | 9430740 |
| premarket evaluation of a commercial glycoprotein g-based enzyme immunoassay for herpes simplex virus type-specific antibodies. | a new commercial glycoprotein g-based enzyme immunoassay (gg-eia) was compared with western blotting (wb) for detection of herpes simplex virus type 1 (hsv-1) or hsv-2 type-specific antibodies in 193 serum samples. sensitivity for hsv-1 was 95%; specificity was 96%. sensitivity for hsv-2 was 98%; specificity was 97%. twelve of 13 serum samples with equivocal gg-eia results were serotyped by wb. | 1998 | 9431971 |
| herpes simplex virus types 1 and 2, chlamydia, syphilis, and toxoplasma in pregnant japanese women with htlv-i. | 1998 | 9436768 | |
| tests of vaginal microbicides in the mouse genital herpes model. | microbicide candidates were selected that have demonstrated activity against sperm or sexually transmitted disease pathogens in vitro, and the efficacy of these agents for preventing vaginal transmission of genital herpes infection was evaluated in the progestin-treated mouse. each agent was delivered to the vaginas of mice approximately 20 sec prior to delivering a highly infectious herpes simplex virus-2 inoculum. the following agents provided significant protection: anti-hsv monoclonal antibo ... | 1997 | 9437563 |
| (z)- and (e)-2-((hydroxymethyl)cyclopropylidene)methyladenine and -guanine. new nucleoside analogues with a broad-spectrum antiviral activity. | new nucleoside analogues 14-17 based on a methylenecyclopropane structure were synthesized and evaluated for antiviral activity. reaction of 2,3-dibromopropene (19) with adenine (18) led to bromoalkene 20, which was benzoylated to give n6,n6-dibenzoyl derivative 23. attempts to convert 20 or 23 to bromocyclopropanes 21 and 22 by reaction with ethyl diazoacetate catalyzed by rh2(oac)4 were futile. by contrast, 2,3-dibromopropene (19) afforded smoothly (e)- and (z)-dibromocyclopropane carboxylic e ... | 1998 | 9438017 |
| herpes simplex virus type-2, cytomegalovirus and epstein-barr virus infection in acute non a to e hepatitis thai patients. | a significant number of acute non a to e hepatitis cases are reported in thailand every year, and the etiologies of these cases are unknown. members of the herpesviridae family have been reported to cause either a self limited or fatal hepatitis in a small proportion of patients in other parts of the world. to determine whether herpesviruses may play a role in acute non a to e hepatitis, sera from 32 acute hepatitis patients without markers for acute hepatitis a to e virus infection were examine ... | 1997 | 9438547 |
| an intertypic herpes simplex virus helicase-primase complex associated with a defect in neurovirulence has reduced primase activity. | r13-1 is an intertypic recombinant virus in which the left-hand 18% of the herpes simplex virus type 1 (hsv-1) genome is replaced by homologous sequences from hsv-2. r13-1 is nonneurovirulent and defective in dna replication in neurons. the defect was localized to the ul5 open reading frame by using marker rescue analysis (d. c. bloom and j. g. stevens, j. virol. 68:3761-3772, 1994). to provide conclusive evidence that ul5 is the only hsv-2 gene involved in the restricted replication phenotype o ... | 1998 | 9445019 |
| herpesvirus entry mediator hvem mediates cell-cell spread in bhk(tk-) cell clones. | 95-19 and u(s)11c119.3 are bhk(tk-)-derived cell lines that are highly resistant to postattachment entry of herpes simplex virus type 1 (hsv-1) and hsv-2 but not to later steps in single-step replication. the resistance properties of these two cell types are not identical. u(s)11c119.3 cells are fully susceptible to pseudorabies virus (prv), as shown by single-step growth experiments, whereas 95-19 cells are resistant to entry of free prv but not to entry by cell-cell spread. we have tested the ... | 1998 | 9445042 |
| herpes simplex virus. | cns infections caused by human herpesvirus 1 and human herpesvirus 2-herpes simplex virus type 1 and herpes simplex virus type 2--are reviewed. the major diseases associated with these viruses are meningitis and encephalitis. two forms of encephalitis are known, neonatal encephalitis and encephalitis occurring after the neonatal period. both diseases are associated with high mortality and morbidity and require prompt diagnosis and aggressive antiviral chemotherapy. methods for the specific diagn ... | 1997 | 9450224 |
| herpetic whitlow of the toe: an unusual manifestation of infection with herpes simplex virus type 2. | 1998 | 9455540 | |
| herpes simplex virus type 2: unique biological properties include neoplastic potential mediated by the pk domain of the large subunit of ribonucleotide reductase. | the prevalence of herpes simplex virus type 2 (hsv-2) infections in the us has increased approximately 30%. like hsv-1, which causes facial lesions, hsv-2 causes symptomatic lesions (at genital sites) and establishes latent infections of the sensory ganglia. however, the two viruses are biologically distinct, suggesting that they possess unique functions which are mediated by different viral genes. unlike hsv-1, hsv-2 is a tumor virus. it causes neoplastic transformation of cultured human cells ... | 1998 | 9459654 |
| light, a new member of the tnf superfamily, and lymphotoxin alpha are ligands for herpesvirus entry mediator. | herpes simplex virus (hsv) 1 and 2 infect activated t lymphocytes by attachment of the hsv envelope glycoprotein d (gd) to the cellular herpesvirus entry mediator (hvem), an orphan member of the tumor necrosis factor receptor superfamily. here, we demonstrate that hvem binds two cellular ligands, secreted lymphotoxin alpha (ltalpha) and light, a new member of the tnf superfamily. light is a 29 kda type ii transmembrane protein produced by activated t cells that also engages the receptor for the ... | 1998 | 9462508 |
| association between human immunodeficiency virus and herpes simplex virus type 2 seropositivity among male factory workers in zimbabwe. | to determine the seroprevalence of herpes simplex virus type 2 (hsv-2), to identify correlates of infection, and to describe the correlation with human immunodeficiency virus (hiv) seropositivity, 224 hiv-negative and 191 hiv-positive male factory workers in zimbabwe were screened for hsv-2-specific antibodies. hsv-2 seroprevalence was 35.7% among hiv-negative subjects and 82.7% among hiv-positive subjects. the weighted estimate of hsv-2 seroprevalence in this population is 44.6%. the correlatio ... | 1998 | 9466543 |
| a novel method to assay herpes simplex virus neutralizing antibodies using bhkicp6lacz-5 (elvis) cells. | a novel method for determining neutralizing serum antibody titers to herpes simplex virus type 2 (hsv-2) was developed based on reduction of infectivity in bhkicp6lacz-5 (elvis) cells; baby hamster kidney (bhk) cells that have been genetically engineered to contain the escherichia coli lacz gene under the control of an inducible herpes simplex virus type 1 (hsv-1) promoter. the test has a semiautomated, colorimetric readout resulting in rapid, objective readings of infectivity reduction. extent ... | 1997 | 9473152 |
| evaluation of antioxidant healing formulations in topical therapy of experimental cutaneous and genital herpes simplex virus infections. | a genital hsv-2 infection of guinea pigs and a cutaneous hsv-1 infection in mice were used to examine the ability of antioxidant components crt1 to reduce lesion development, duration, and severity. crt is a patented antioxidant formulation developed by warner-lambert worldwide consumer healthcare r. and d. crt contains three components that work synergistically: vitamin e, sodium pyruvate and membrane stabilizing fatty acids. the ms strain of hsv-2 was utilized for intravaginal inoculation of g ... | 1997 | 9477116 |
| humoral and cellular immune responses to herpes simplex virus-2 glycoprotein d generated by facilitated dna immunization of mice. | 1998 | 9479837 | |
| herpes simplex virus-2 infection. an emerging disease? | genital herpes infections continue to increase in the world. as outlined previously, several factors play a role in the continued transmission of genital herpes worldwide. reducing the medical consequences of hsv-2 infection will not be easy. it is also sobering to realize that widespread penetration of hsv-2 into the population had preceded the spread of sexually acquired hiv in most countries. the biological rationale for a connection between hiv and hsv continues to be strengthened. currently ... | 1998 | 9494829 |
| a peptide binding motif for hla-dqa1*0102/dqb1*0602, the class ii mhc molecule associated with dominant protection in insulin-dependent diabetes mellitus. | hla-dqa1*0102/dqb1*0602 (dq0602) is observed at a decreased frequency in insulin-dependent diabetes mellitus in different ethnic groups, suggesting a protective role for dq0602. analysis of overlapping peptides from human insulin found that insulin b(1-15) bound well to dq0602 and exhibited a high degree of allelic specificity. truncation analysis of insulin b(1-15) identified insulin b(5-15) as the minimal peptide for dq0602 binding. insulin b(5-15) bound to dq0602 with an apparent kd of 0.7 to ... | 1998 | 9498778 |
| the genome sequence of herpes simplex virus type 2. | the genomic dna sequence of herpes simplex virus type 2 (hsv-2) strain hg52 was determined as 154,746 bp with a g+c content of 70.4%. a total of 74 genes encoding distinct proteins was identified; three of these were each present in two copies, within major repeat elements of the genome. the hsv-2 gene set corresponds closely with that of hsv-1, and the hsv-2 sequence prompted several local revisions to the published hsv-1 sequence (d. j. mcgeoch, m. a. dalrymple, a. j. davison, a. dolan, m. c. ... | 1998 | 9499055 |
| inducible overexpression of a toxic protein by an adenovirus vector with a tetracycline-regulatable expression cassette. | we have constructed two new adenovirus expression cassettes that expand both the range of genes which can be expressed with adenovirus vectors (adv) and the range of cells in which high-level expression can be attained. by inclusion of a tetracycline-regulated promoter in the transfer vector padtr5, it is now possible to generate recombinant adenoviruses expressing proteins that are either cytotoxic or that interfere with adenovirus replication. we have used this strategy to generate a recombina ... | 1998 | 9499088 |
| herpes simplex virus type 2 icp47 inhibits human tap but not mouse tap. | herpes simplex virus serotype 1 (hsv-1) expresses an immediate-early protein, icp47, that effectively blocks the major histocompatibility complex class i antigen presentation pathway. hsv-1 icp47 (icp47-1) binds with high affinity to the human transporter associated with antigen presentation (tap) and blocks the binding of antigenic peptides. hsv type 2 (hsv-2) icp47 (icp47-2) has only 42% amino acid sequence identity with icp47-1. here, we compared the levels of inhibition of human and murine t ... | 1998 | 9499125 |
| recent advances in vaccine adjuvants for systemic and mucosal administration. | although vaccines produced by recombinant dna technology are safer than traditional vaccines, which are based on attenuated or inactivated bacteria or viruses, they are often poorly immunogenic. therefore, adjuvants are often required to enhance the immunogenicity of these vaccines. a number of adjuvants which are particulates of defined dimensions (<5 microm) have been shown to be effective in enhancing the immunogenicity of weak antigens in animal models. two novel adjuvants which possess sign ... | 1998 | 9504429 |
| prevalence of hsv-2 antibodies among std clinic patients in tanzania. | patients attending a referral sexually transmissible diseases clinic at muhimbili medical centre in dar-es-salaam during the period 1989 to 1993 were examined for herpes simplex virus type 2 (hsv-2) antibodies. an elisa technique, using glycoprotein g of hsv-2 as antigen, was used to test 294 patients' sera. of these, 126 sera were hsv-2 positive, while 168 were negative, yielding an overall hsv-2 prevalence of 42.9%. sixty-three per cent of the women and 35.5% of the men were hsv-2 positive. se ... | 1998 | 9506376 |
| comparison of two enzyme-linked immunosorbent assays and one rapid immunoblot assay for detection of herpes simplex virus type 2-specific antibodies in serum. | the sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (hsv-2)-specific immunoglobulin g antibodies in serum, including the one-strip rapid immunoblot assay (riba; chiron corporation) and two indirect enzyme immunosorbent assays (eia; gull laboratories and centocor), were compared by testing a panel of 1,250 serum samples from individuals attending an outpatient clinic for sexually transmitted diseases. a qualitative agreement among the three a ... | 1998 | 9508330 |
| detection and direct typing of herpes simplex virus in perianal ulcers of patients with aids by pcr. | the presence of herpes simplex virus type 1 (hsv-1) and hsv-2 in perianal ulcerations of 41 aids patients was assessed by virus culture and a type-specific pcr-based assay. hsv was isolated from the lesion site in 24 of 41 (58.5%) patients, and hsv dna was detected by pcr in all 24 (100%) of these specimens. additionally, pcr was used to detect hsv dna in 12 of 17 (70.5%) hsv culture-negative samples. thus, hsv genomic sequences could be demonstrated in 36 of 41 (87.8%) perianal ulcers in this s ... | 1998 | 9508331 |
| [herpetic keratitis: clinical-virological correlation]. | herpetic keratitis is the main infectious cause of corneal opacity. the existence of effective antiviral agents underscores the need of an early diagnosis. | 1997 | 9515284 |
| experimental myelitis caused by herpes simplex virus type 2 in c57bl/6n and balb/cn mice. | intraperitoneal and intracranial inoculation of herpes simplex virus type 2 (hsv 2) into balb/cn and c57bl/6n mice was carried out to induce experimental myelitis. the myelitis was clearly observed in c57bl/6n mice following intraperitoneal inoculation. within 24 hours before death, the mice showed urinary and rectal incontinence and paraplegia of the hind legs. randomly distributed, severe necrosis was demonstrated in the spinal cord, mainly at the lower cord. in balb/cn mice the clinical sympt ... | 1997 | 9516872 |
| detection of herpes simplex virus (hsv) in aborted material using the polymerase chain reaction technique. | to investigate the contribution of hsv to the aetiopathogenesis of spontaneous abortion. | 1998 | 9517803 |
| the bglii-n fragment of herpes simplex virus type 2 contains a region responsible for resistance to antiviral effects of interferon. | double infection with two interferon (ifn)-sensitive strains of herpes simplex virus (hsv), hsv-1(17syn) and hsv-2(uw268), showed reduced inhibition of virus growth by ifn. intertypic recombinants with ifn resistance were obtained from the doubly infected cultures. these results indicate that hsv ifn resistance is controlled by at least two genetic regions. restriction endonuclease analysis demonstrated that the recombinants were similar to hsv-2 in their genomic structure but the bamhi-a, bglii ... | 1998 | 9519835 |