Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| enhancement of hsv-dna infectivity, in vero and rs cells, by a modified calcium-phosphate transfection technique. | infectivity of herpes simplex virus (hsv) dna was assessed by employing the calcium-phosphate transfection technique described by chen and okayama, originally applied to increase the efficiency of plasmid transfection by n, n-bis (2-hydroxyethyl)-2-aminoethane sulfonic acid (bes). the experimental conditions and efficiency of this transfection procedure were evaluated comparing the viral progeny titers obtained by the chen and okayama transfection method using dna from wild-type strains of hsv-1 ... | 1996 | 8879137 |
| meisenheimer rearrangement of azetopyridoindoles. viii. synthesis and antiviral activities of 12-carbaeudistomin analogs. | eudistomins, isolated from the colonial tunicate eudistoma olivaceum, have been a synthetic target due to their strong antiviral activity against herpes simplex virus (hsv-1) and activities against certain types of tumors in vivo. in order to examine the structure-activity relationship of eudistomins, 12-carbaeudistomin analogs were synthesized and their activities against influenza a and b virus, hsv-1, hsv-2 and human cytomegalovirus were investigated. among them, racemic 6-methoxy-12-carbaeud ... | 1996 | 8689725 |
| comparison of a dna probe assay with the plaque reduction assay for measuring the sensitivity of herpes simplex virus and varicella-zoster virus to penciclovir and acyclovir. | a dna probe assay was compared with the plaque reduction assay to determine the sensitivity of clinical isolates of herpes simplex virus (hsv) and varicella-zoster virus (vzv) to penciclovir and acyclovir in mrc-5 cells. in both assays, penciclovir and acyclovir shared comparable activity against cell-free virus (cfv) preparations of vzv and herpes simplex virus type 1 (hsv-1) isolates, whilst acyclovir was significantly more active than penciclovir against herpes simplex virus type 2 (hsv-2) is ... | 1996 | 8690764 |
| therapeutic potential of cidofovir (hpmpc, vistide) for the treatment of dna virus (i.e. herpes-, papova-, pox- and adenovirus) infections. | (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc, cidofovir, vistide) is an acyclic nucleoside phosphonate with broad-spectrum activity against a wide variety of dna viruses including herpesviruses [herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv), human herpesvirus type 6 (hhv-6) and equine and bovine herpesviruses], papovaviruses [human polyoma virus and human papilloma virus (hpv)], adeno-, irido-, ... | 1996 | 8701600 |
| peptide sequences of glycoprotein g-2 discriminate between herpes simplex virus type 2 (hsv-2) and hsv-1 antibodies. | the complete herpes simplex virus type 2 envelope glycoprotein g was represented by overlapping synthetic peptides. herpes simplex virus type 2-specific human seroreactivities were mainly seen against three peptides, peptides g2-64, g2-69, and g2-70, located in the c-terminal part of glycoprotein g. this is, interestingly, a region which has strong homology between herpes simplex virus types 1 and 2. g2-69 was the most herpes simplex virus type 2-specific peptide, reacting with 93% (13 of 14) of ... | 1996 | 8705666 |
| isolation of pyropheophorbide a from the leaves of atalantia monophylla (roxb.) corr. (rutaceae) as a possible antiviral active principle against herpes simplex virus type 2. | antiviral activity-guided isolation studies on the leaves of atalantia monophylla (roxb.) corr. (rutaceae) led to the identification of pyropheophorbide a (1), a simple chlorin derivative, from the chloroform extract (fr. b) as a possible antiviral active principle against herpes simplex virus type 2 (hsv-2). pyropheophorbide a methyl ester (2) was also isolated from the hexane extract (fr. a). | 1996 | 8706147 |
| a comparison of referral patterns and characteristics of patients with first episode symptomatic genital hsv-1 and hsv-2 infections in sheffield. | to ascertain factors associated with hsv-1 and hsv-2 isolates in patients attending a genitourinary medicine clinic with symptomatic first episode genital herpes (fegh). | 1996 | 8707325 |
| activity of penciclovir in antiviral assays against herpes simplex virus. | the effect of penciclovir and acyclovir on the replication of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) strains was determined in mrc-5 cells infected with 0.01 pfu/cell and exposed to the drugs for 72 h to allow multiple cycles of replication. penciclovir was significantly more active than acyclovir against three strains of hsv-1 and three strains of hsv-2 at 1 mg/l (p = 0.009), 3 mg/l (p < 0.001) and 10 mg/l (p = 0.001). further comparisons between the compounds were made in mrc-5 ... | 1996 | 8707740 |
| highly virulent strains of herpes simplex virus fail to kill mice following infection via gingival route. | virulence of herpes simplex virus (hsv) in mice has been demonstrated to be dependent on the site of infection. in this experiment, pathogenesis of hsv was studied in 2 different routes of infection in a mouse model system. when balb/c mice were infected with 5 x 10(3) plaque-forming units (pfu) of virulent hsv type 1 miyama gc+ strain (hsv-1-gc+) intraperitoneally, all mice were dead in 6 to 9 days. hsv-1-gc+ was recovered from organs such as the cerebrum, cerebellum, brainstem, and spleen 2 to ... | 1996 | 8708138 |
| herpes encephalitis is a disease of middle aged and elderly people: polymerase chain reaction for detection of herpes simplex virus in the csf of 516 patients with encephalitis. the study group. | to assess the diagnostic potential of the polymerase chain reaction (pcr) in herpes simplex virus (hsv) encephalitis. | 1996 | 8708648 |
| immunohistochemical studies on the transneuronal spread of virulent herpes simplex virus type 2 and its us3 protein kinase-deficient mutant after ocular inoculation. | the transneuronal spread of a virulent wild-type herpes simplex virus type 2 (hsv-2) and its us3 protein kinase-deficient (us3 pk-) mutant was immunohistochemically studied in mice after inoculations into the cornea, anterior chamber, tongue, and masseter muscle. after corneal inoculation, the wild-type virus was demonstrated in various brain stem areas including the trigeminal tract and nucleus, the reticular formation, and cerebellar nucleus group. viral antigen-positive neurons were strictly ... | 1996 | 8709864 |
| induction of cytotoxic t lymphocytes by intramuscular immunization with plasmid dna is facilitated by bone marrow-derived cells. | striated muscle is the predominant site of gene expression after i.m. immunization of plasmid dna, but it is not clear if myocytes or professional antigen-presenting cells (apcs) of hematopoietic origin present the encoded antigens to class i major histocompatibility complex (mhc)-restricted cytotoxic t lymphocytes (ctl). to address this issue, ctl responses were assessed in mice engrafted with immune systems that were partially mhc matched with antigen-producing muscle cells. spleen cells (sc) ... | 1996 | 8710913 |
| gender differences in sexual practices and sexually transmitted infections among adults in lima, peru. | this study examined the prevalences of antibodies to treponema pallidum, chlamydia trachomatis, and herpes simplex virus type 2 in a sample of peruvian adults. | 1996 | 8712268 |
| deletion of the carboxy-terminus of herpes simplex virus type 1 (hsv-1) glycoprotein b does not affect oligomerization, heparin-binding activity, or its ability to protect against hsv challenge. | a recombinant vaccinia virus designated vgbt which expresses a truncated secreted herpes simplex virus gb (gbt) was constructed and compared to v11gb, a vaccinia recombinant previously studied which expresses gb exclusively on the surface of infected cells. indirect immunofluorescence assay (ifa) revealed that gbt was strongly associated with the surface of infected cells despite being released slowly into the cell culture medium. both gb and gbt existed as oligomers, and both membrane bound and ... | 1996 | 8712932 |
| simultaneous pcr detection of haemophilus ducreyi, treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers. | a multiplex pcr (m-pcr) assay with colorimetric detection was devised for the simultaneous amplification of dna targets from haemophilus ducreyi, treponema pallidum, and herpes simplex virus (hsv) types 1 and 2. by using target-specific oligonucleotides in a microwell format, 298 genital ulcer swab specimens collected in new orleans during three intervals from 1992 through 1994 were evaluated. the results of the m-pcr assay were compared with the results of dark-field microscopy and h. ducreyi c ... | 1996 | 8748271 |
| [the relationship between placental pathology and certain pathogens detected in paraffin-embedded tissues in cases of intrauterine growth retardation]. | to study the relationship between placental pathology and certain pathogens detected in paraffin-embedded placental tissues in cases of intrauterine growth retardation (iugr). | 1996 | 8758801 |
| the characteristic site-specific reactivation phenotypes of hsv-1 and hsv-2 depend upon the latency-associated transcript region. | after replication at sites of initial inoculation, herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) establish lifelong latent infections of the sensory and autonomic neurons of the ganglia serving those sites. periodically, the virus reactivates from these neurons, and travels centripetally along the neuronal axon to cause recurrent epithelial infection. the major clinically observed difference between infections with herpes simplex virus type 1 and type 2 is the anatomic site specificity of ... | 1996 | 8760819 |
| disulfide bond structure determination and biochemical analysis of glycoprotein c from herpes simplex virus. | a biochemical analysis of glycoprotein c (gc of herpes simplex virus was undertaken to further characterize the structure of the glycoprotein and to determine its disulfide bond arrangement. we used three recombinant forms of gc, gc1(457t), gc1(delta33-123t), and gc2(426t), each truncated prior to the transmembrane region. the proteins were expressed and secreted by using a baculovirus expression system and have been shown to bind to monoclonal antibodies which recognize discontinuous epitopes a ... | 1996 | 8764057 |
| synthesis of 2'-aminomethyl derivatives of n-(2-(phosphonomethoxy)ethyl) nucleotide analogues as potential antiviral agents. | a series of purine and pyrimidine n-(2-(phosphonomethoxy)ethyl) derivatives bearing aminomethyl, (dimethylamino)methyl, morpholinomethyl, and (trimethylammonio)methyl groups at the 2'-position were synthesized. the compounds were prepared by alkylation of the heterocyclic bases with appropriately substituted (aminoalkyl)oxiranes followed by condensation of the resulting intermediates with dialkyl ((p-tolylsulfonyl)oxy)methanephosphonate and subsequent treatment of the obtained diester with bromo ... | 1996 | 8765509 |
| synthesis and antiviral activity evaluation of some new aminoadamantane derivatives. 2. | the synthesis of some new aminoadamantane derivatives is described. the new compounds were evaluated against a wide range of viruses [influenza a h1n1, influenza a h2n2, influenza a h3n2, influenza b, parainfluenza 3, herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), thymidine kinase-deficient (tk-) hsv-1, vaccinia, vesicular stomatitis, polio 1, coxsackie b4, sindbis, semliki forest, reo 1, varicella-zoster virus (vzv), tk- vzv, human cytomegalovirus (hcmv), and human immunodeficiency vir ... | 1996 | 8765514 |
| relationship between antibodies to herpes simplex virus (hsv) and symptoms of hsv infection. | to determine the relationship between antibodies to herpes simplex virus (hsv) types 1 and 2 and diagnosis of orolabial and genital herpes, a cross-sectional survey was done among 869 sexually transmitted disease clinic attendees and 1594 blood donors in london. among clinic attenders, the prevalence of hsv-1 infection was 59.5% and that of hsv-2 infection was 22.7%, and among blood donors the prevalence was 44.6% and 7.6%, respectively. the sensitivity and specificity of a diagnosis of oral her ... | 1996 | 8769602 |
| spontaneous reactivation of thymidine kinase-deficient, acyclovir-resistant type-2 herpes simplex virus: masked heterogeneity or reversion? | herpes simplex virus (hsv) strain 1737, acyclovir-resistant and uniformly thymidine kinase-deficient (tkd) by all conventional assays, clinically reactivated in an aids patient in the absence of antiviral drug pressure. investigation of its neurovirulence and latency characteristics in a mouse model using a tkd plaque isolate (1737-14), however, yielded a neurovirulent, homogeneous, acyclovir-sensitive, tk wild type (tkwt) strain (1737-14me), while trigeminal ganglia from a surviving animal yiel ... | 1996 | 8769603 |
| antiviral properties of extract of opuntia streptacantha. | an extract of the cactus plant opuntia streptacantha inhibited intracellular virus replication and inactivated extracellular virus. inhibition of virus replication also occurred following pre-infection treatment--a favourable finding in terms of in-vivo limitation of virus disease. there was inhibition of both dna and rna virus replication, for example, herpes simplex virus, equine herpes virus, pseudorabies virus, influenza virus, respiratory syncytial virus and human immunodeficiency virus, wi ... | 1996 | 8783800 |
| detection of a transforming fragment of herpes simplex virus type 2 in clinical specimens by pcr. the canadian women's hiv study group. | a pcr assay for the sensitive detection of a transforming fragment of herpes simplex virus type 2 (hsv-2) was developed. oligonucleotide primers were selected in xho-2, a transforming region of the bglii n fragment of hsv-2. the assay reached a sensitivity endpoint of 10 copies of the xho-2 subfragment and did not show cross-reactivity with other herpesviruses, including hsv-1. all 42 hsv-2 isolates scored positive in the assay. the xho-2 pcr assay was evaluated with 216 clinical specimens and t ... | 1996 | 8784564 |
| disseminated herpes simplex virus infection presenting as fever in the newborn--a lethal outcome. | the clinical course of a neonate who presented with fever and tachypnea on day 6 of life is described. he developed disseminated intravascular coagulopathy, hepatic failure, coma and expired at 14 days of age. the post mortem viral cultures from liver, adrenal and lungs were positive for hsv type 2. the fatal outcome of this case of fever, due to hsv infection, emphasizes the need for early treatment of suspicious cases of hsv infection. hsv should be considered in the differential diagnosis of ... | 1996 | 8793716 |
| recurring episodes of meningitis (mollaret's meningitis) with one showing an association with herpes simplex virus type 2. | benign recurrent aseptic (mollaret's) meningitis is a rare disease of unknown aetiology. we report the case of a 27-year-old woman who experienced three episodes of lymphocytic meningitis. human simplex virus (hsv) type 2 dna was detected in the csf by pcr amplification indicating the diagnosis of recurrent hsv type 2 meningitis. our observation suggests that search of herpes virus dna by pcr amplification on csf may be useful in unexplained recurrent meningitis. | 1996 | 8793718 |
| detection of herpes simplex virus dna in cerebrospinal fluid samples using the polymerase chain reaction and microplate hybridization. | as conventional polymerase chain reaction (pcr) procedures are time-consuming and laborious, we developed and evaluated a rapid semi-automatic microplate method to detect the amplified pcr products. the use of pcr, with subsequent hybridization in microplates, is described for the detection of herpes simplex virus (hsv) dna in cerebrospinal fluid samples. the principle of the method is based on two phases. firstly, the amplification of the viral dna in the sample is undertaken using a pair of pr ... | 1996 | 8793825 |
| new antiherpesvirus agents. their targets and therapeutic potential. | of the large number of agents under development for the treatment of herpes virus infections [herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), varicella zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv)], only ten have apparently reached clinical development. aciclovir was approved for the treatment of hsv infections over 10 years ago, and it remains an important and reliable antiviral agent. recent approvals in some countries of valaciclovir for vzv infection and famciclo ... | 1996 | 8799682 |
| effect of age and route of inoculation on outcome of neonatal herpes simplex virus infection in guinea pigs. | the morbidity and mortality of neonatal herpes simplex virus infection remains unacceptably high despite antiviral therapy. a better understanding of factors that might contribute to this poor outcome is needed but has been hindered by a lack of a good animal model. the recently described guinea pig model of neonatal hsv-2 infection was used to explore the effect of age and route of inoculation on the outcome of infection. after intranasal inoculation the onset, extent, and severity of the prima ... | 1996 | 8801285 |
| epidemiology of herpes simplex virus type 2 infections in a high-risk adolescent population. | the seroprevalence of infection with type 2 herpes simplex virus (hsv-2) was determined in 135 adolescents detained in a juvenile detention facility. a total of 16% of enrollees were seropositive for hsv-2. age of onset of sexual intercourse, number of lifetime partners, frequency of condom use, and history of sexually transmitted diseases did not predict hsv-2 seropositivity. | 1996 | 8803729 |
| evaluation of antiherpetic compounds using a gastric cancer cell line: pronounced activity of bvdu against herpes simplex virus replication. | we developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (hsv) in a model of gastrointestinal herpetic infection in vitro. this method was based on inhibition of hsv-induced cytopathogenicity in gastric adenocarcinoma mkn-28 cells, as monitored by an mtt colorimetric assay. from the various compounds that were evaluated for their activity against hsv-1 and hsv-2, brivudine (bvdu) emerged as the most effective. when the 50% effective concentration ... | 1996 | 8805100 |
| unique fold and active site in cytomegalovirus protease. | human herpesviruses are responsible for a variety of diseases. they are divided into three subfamilies: alpha includes herpes simplex viruses (hsv-1 and hsv-2) and varicella-zoster virus (vzv); beta includes cytomegalovirus (cmv) and human herpesvirus-6 (hhv-6); and gamma includes epstein-barr virus (ebv). each virus encodes a serine protease that is essential for its replication and is a potential target for therapeutic intervention. human cmv is a ubiquitous opportunistic pathogen that can res ... | 1996 | 8805707 |
| cellular uptake and biological effects of antisense oligodeoxynucleotide analogs targeted to herpes simplex virus. | in this study, we synthesized antisense oligodeoxynucleotides (odns) with phosphodiester, phosphorothioate (s-odns), or methylphosphonate linkages complementary to the splicing acceptor site of immediate-early pre-mrna 5 of herpes simplex virus type 1 (hsv-1). the antiviral activity of each analog on cytopathic effect in cells infected with hsv-1 or hsv-2 was assessed and compared with the cellular uptake of the analog. we found that antisense s-odns showed the most potent antiherpetic activity, ... | 1996 | 8807060 |
| nucleosides with a twist. can fixed forms of sugar ring pucker influence biological activity in nucleosides and oligonucleotides? | the sugar moiety of nucleosides in solution is known to exist in a rapid dynamic equilibrium between extreme northern and southern conformations as defined in the pseudorotational cycle. in the present work, we describe how the bicyclo[3.1.0]hexane template fixes the ring pucker of 2'-deoxy-methanocarba-nucleosides 1-5 and 12 to values corresponding to either one of these two extreme conformations that are typical of nucleosides. the syntheses of the fixed northern conformers 1-5 were performed ... | 1996 | 8809162 |
| subclinical shedding of hsv: its potential for reduction by antiviral therapy. | 1996 | 8815678 | |
| seroepidemiology and control of genital herpes: the value of type specific antibodies to herpes simplex virus. | herpes simplex virus (hsv) type 2 is the main cause of genital herpes infections. conventional serological techniques cannot discriminate between antibodies specific to hsv types 1 and 2, so these methods cannot be used in the seroepidemiological investigation of genital herpes infections. the discovery of type specific antigens in hsv-1 and hsv-2, most notably glycoprotein g (gg), has been successfully exploited to develop assays for the detection of type specific hsv antibody. the presence of ... | 1996 | 8820192 |
| disseminated herpesvirus infection during pregnancy. | a case of disseminated herpes simplex virus infection during pregnancy is reported, and 26 similar cases are reviewed. the present case was unusual in that it occurred in the 12th week of gestation, whereas all but one of the other cases occurred in the second or third trimester. this rare complication is generally preceded by a flu-like prodrome with or without oral or genital lesions. the clinical presentation is (1) encephalitis without hepatic involvement, (2) hepatitis with or without centr ... | 1996 | 8824966 |
| inoculation herpes simplex virus infections in patients with aids: unusual appearance and location of lesions. | two patients with aids developed protracted infection due to autoinoculation of herpes simplex virus in the great toe and the external ear, respectively, both unusual areas for inoculation. the appearances of the lesions were also unusual; severe hyperkeratosis was noted in both cases and a mass lesion in the external ear in one case. both patients' conditions responded to acyclovir, although one patient required amputation of a digit due to intractable pain. in each case, the diagnosis was dela ... | 1996 | 8824981 |
| lack of detection of hsv dna in pbmcs and lymph nodes of hiv-infected persons. | 1996 | 8825709 | |
| the relationship of human papillomavirus-related cervical tumors to cigarette smoking, oral contraceptive use, and prior herpes simplex virus type 2 infection. | it has now been established that infection with human papillomavirus (hpv) is necessary for the development of most cervical cancers. hpv is not sufficient for the development of cancer. other exposures or host factors are necessary for cancer to occur. as part of an ongoing, population-based case-control study of invasive cervical cancer, we investigated the role of cigarette smoking, oral contraceptive (oc) use, and herpes simplex virus type 2 (hsv-2) as potential cofactors with hpv in the dev ... | 1996 | 8827359 |
| acyclic nucleotide analogs derived from 8-azapurines: synthesis and antiviral activity. | reaction of phosphoroorganic synthons with 8-azaadenine, 8-aza-2, 6-diaminopurine, and 8-azaguanine using cesium carbonate yielded regioisomeric 8-azapurine n7-, n8-, and n9-(2-(phosphonomethoxy)alkyl) derivatives. this reaction followed by deprotection afforded isomeric 2-(phosphonomethoxy)ethyl (pme), (s)-(3-hydroxy-2-(phosphonomethoxy)propyl) [(s)-hpmp], (s)-(3-flouro-2-(phosphonomethoxy)propyl) [(s)-fpmp], (s)-(2-(phosphonomethoxy)propyl) [(s)-pmp], and (r)-(2-(phosphonomethoxy)propyl) [(r)- ... | 1996 | 8831773 |
| herpetic croup: two case reports and a review of the literature. | croup is an acute infectious illness usually occurring in children; it is characterized by brassy cough and stridor. the main pathogens include mainly parainfluenza and influenza viruses. recently there have been reports of prolonged croup caused by the herpes simplex viruses. we report two cases of prolonged croup due to herpes simplex types 1 and 2. we also review and summarize the reported pediatric cases of herpetic croup. | 1996 | 8834994 |
| a case of varicella-zoster myelopathy. | early diagnosis of neurological complications of varicella-zoster virus (vzv) is important because of its treatability. we performed polymerase chain reaction (pcr) to detect vzv-dna from the cerebrospinal fluid (csf) of a patient with myelopathy. | 1996 | 8836311 |
| the pharmacological profile of famciclovir. | famciclovir is the well-absorbed oral form of penciclovir, a potent and selective antiviral agent, with activity against members of the herpesvirus family, including varicella-zoster virus (vzv), and herpes simplex virus-1 (hsv-1) and hsv-2. famciclovir is rapidly absorbed and converted to penciclovir. penciclovir has excellent bioavailability (77%) after oral administration of 500 mg of famciclovir. similar to acyclovir, famciclovir is converted by phosphorylation to its active metabolite, penc ... | 1996 | 8840412 |
| a short-term plaque assay for antiviral drug research on herpes simplex virus type 2. | in antiviral drug research, gelatinized growth medium cannot be used after infection to avoid the development of secondary plaques, because agar or other chemicals may interfere with the assessment of cytotoxic activity of antiviral compounds. in this study we evaluated quantitatively the different expression of cytopathic effects of herpes simplex virus type 2 (hsv-2) in vero cell cultures utilizing different periods of incubation after infection, and analysed the incidence of viral progeny par ... | 1996 | 8841042 |
| in vitro study of a double infection by herpes simplex virus type 2 and chlamydia trachomatis. | sexually transmitted diseases (stds) may recognize multiple etiological agents. among them, chlamydia trachomatis (ct) and herpes simplex virus type 2 (hsv-2) cause symptomatic, subclinical and asymptomatic infections of the urogenital tract which can lead to serious sequelae. in the present study the coinfection and superinfection by ct and hsv-2 in epithelial cultured cells from human cervix (hela 229) are described. a double infection, followed by the intracellular synthesis of chlamydial and ... | 1996 | 8841043 |
| effect of antibody alone and combined with acyclovir on neonatal herpes simplex virus infection in guinea pigs. | neonatal herpes simplex virus (hsv) infection produces severe disease with unacceptable morbidity and mortality with current antiviral therapies. the effect of therapy with passive anti-hsv antibody and acyclovir was evaluated using a guinea pig model of neonatal hsv. newborn animals were inoculated intranasally and treated with acyclovir (60 mg/kg/day) or antibody (or both), beginning on days 0, 2, or 3 after hsv-2 inoculation. acyclovir alone was effective only when begun on day 0, and antibod ... | 1996 | 8537645 |
| herpes simplex virus type 2 infection associated with urinary retention in the absence of genital lesions. | a 14-year-old boy had inguinal lymphadenitis and associated urinary retention; herpetic genital and anal lesions were absent. lymph node biopsy established the presence of infection with herpes simplex virus type 2. we recommend that this infection be considered in the differential diagnosis of adolescent patients with urinary retention and constipation, even when the diagnostic clue provided by genital ulcers is absent. | 1996 | 8636823 |
| a herpesvirus inhibitor from bovine whey. | 1996 | 8643002 | |
| serologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis. | infectious uveitis entities are usually rapidly progressive blinding diseases that can be prevented by prompt administration of specific antimicrobial therapy. with the aim of improving early diagnosis in patients with infectious uveitis, intraocular fluid samples from patients with sight-threatening posterior uveitis were investigated to determine the causative agent. | 1996 | 8644808 |
| the activity of plant-derived antiretroviral proteins map30 and gap31 against herpes simplex virus in vitro. | we examined the effect on anti-hiv proteins map30 and gap31, from momordica charantia and gelonium multiflorum, on the infection and replication of herpes simplex viruses (hsv). human lung wi-38 fibroblasts cultured in the presence of tenfold dilutions of map30 or gap31 were exposed to hsv and viral yield was measured at 24-48 hours by elisa. the effective concentrations for 50% inhibitions (ec50) were 0.1-0.2 microm for hsv-2, and 0.3-0.5 microm for hsv-1 for map30 and gap31, respectively. in c ... | 1996 | 8645280 |
| differences in the susceptibility of herpes simplex virus types 1 and 2 to modified heparin compounds suggest serotype differences in viral entry. | although heparan sulfate (hs) serves as an initial receptor for the binding of both herpes simplex virus type 1 (hsv-1) and hsv-2 to cell surfaces, the two serotypes differ in epidemiology, cell tropism, and ability to compete for viral receptors in vitro. these observations are not necessarily contradictory and can be explained if the two serotypes recognize different structural features of hs. to compare the specific features of hs important for the binding and infection of hsv-1 and hsv-2, we ... | 1996 | 8648678 |
| structure-function analysis of soluble forms of herpes simplex virus glycoprotein d. | glycoprotein d (gd) of herpes simplex virus (hsv) is essential for virus entry. truncated forms of gd lacking the transmembrane and cytoplasmic tail regions have been shown to bind to cells and block plaque formation. using complementation analysis and a panel of gd mutants, we previously identified four regions of gd (regions i to iv) which are important for virus entry. here, we used baculovirus vectors to overexpress truncated forms of wild-type gd from hsv type 1 (hsv-1) [gd-1(306t)] and hsv ... | 1996 | 8648717 |
| the herpes simplex virus type 2 strain hg52 rl1 gene contains a 154 bp intron as predicted from sequence analysis. | the published sequence of the herpes simplex virus (hsv) type 2 strain hg52 neurovirulence gene rl1 indicated that, unlike the hsv-1 homologue, the gene contains a 154 bp intron. this intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. by rt-pcr of poly(a)+ rna, using primers on either side of the proposed intron, we have isolated a partial cdna clone corresponding to the predicted spliced mrna. | 1996 | 8601785 |
| asymptomatic maternal shedding of herpes simplex virus at the onset of labor: relationship to preterm labor. | to determine if fetal growth restriction and prematurity are observed with subclinical shedding of herpes simplex virus (hsv) at the onset of labor. | 1996 | 8602295 |
| dna immunization against experimental genital herpes simplex virus infection. | a nucleic acid vaccine, expressing the gene encoding herpes simplex virus (hsv) type 2 glycoprotein d (gd2) under control of the cytomegalovirus immediate-early gene promoter, was used to immunize guinea pigs against genital hsv-2 infection. the vaccine elicited humoral immune responses comparable to those seen after hsv-2 infection. immunized animals exhibited protection from primary genital hsv-2 disease with little or no development of vesicular skin lesions and significantly reduced hsv-2 re ... | 1996 | 8603957 |
| the protein kinase activity of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) fused to the extracellular domain of the epidermal growth factor receptor is ligand-inducible. | the gene coding for the large subunit of herpes simplex virus type 2 ribonucleotide reductase (rr) (icp10) has a unique 5' terminal domain the product of which has a serine/threonine (ser/thr) protein kinase (pk) catalytic domain preceded by a transmembrane (tm) segment. because icp10 localizes on the cell surface and is internalized by the endocytic pathway like an activated growth factor receptor (hunter et al., 1995, virology 210, 345-360), we asked whether it is ligand-inducible in order to ... | 1996 | 8610433 |
| interleukin-11: stimulation in vivo and in vitro by respiratory viruses and induction of airways hyperresponsiveness. | to address the role of il-11 in viral airways dysfunction, we determined whether infectious agents that exacerbate asthma stimulate stromal cell il-11 production, determined whether il-11 could be detected at sites of viral infection and evaluated the effects of il-11 on airway physiology. respiratory syncytial virus (rsv), parainfluenza virus type 3 (piv3), and rhinovirus (rv) 14 were potent stimulators while cytomegalovirus and adenovirus only weakly stimulated and herpes simplex virus type 2 ... | 1996 | 8613544 |
| the novel protein kinase of the rr1 subunit of herpes simplex virus has autophosphorylation and transphosphorylation activity that differs in its atp requirements for hsv-1 and hsv-2. | the large subunit of herpes simplex virus (hsv) ribonucleotide reductase (rr1) designated icp6 and icp10 for hsv-1 and hsv-2, respectively, has a novel protein kinase (pk) enzymatic activity. icp10 is localized on the cell surface, a localization that depends on an intact transmembrane (tm) segment. we used immunocomplex pk assays to examine the pk activity of icp10 in stably transfected eukaryotic cells. activity was distinct from that of casein kinase ii (ckii) in that it did not require monov ... | 1996 | 8614985 |
| characterization of an essential hsv-1 protein encoded by the ul25 gene reported to be involved in virus penetration and capsid assembly. | the 2.3-kb bamhi-u dna fragment (map units 0.319-0.335) of herpes simplex virus type 1 (hsv-1) genome contains the complete ul25 open reading frame (orf). it specifies an essential viral protein reported previously to be involved in virus penetration and capsid assembly (c. addison, f. j. rixon, j. w. palfreyman, m. o'hara, and v. g. preston, virology 138, 246-259, 1984). to identify the protein encoded by the ul25 gene, the ul25 orf was cloned in a eukaryotic expression vector (p91023) downstre ... | 1996 | 8615003 |
| human herpes simplex virus (hsv)-specific cd8+ ctl clones recognize hsv-2-infected fibroblasts after treatment with ifn-gamma or when virion host shutoff functions are disabled. | herpes simplex virus (hsv)-specific cd8+ ctl cloned from individuals infected with hsv-2 efficiently lyse hsv-infected ebv-transformed b lymphoblastoid cells; however, these same ctl fail to lyse infected dermal fibroblasts. by 3 h after infection (early), class i mhc expression is reduced to less than 20% of that in uninfected fibroblasts, and expression is further reduced to less than 1% of the level in uninfected cells between 6 and 18 h after infection (late). we used an hsv-2 mutant that la ... | 1996 | 8621929 |
| karalicin, a new biologically active compound from pseudomonas fluorescens/putida. ii. biological properties. | the biological activities of karalicin, a new product from the pseudomonas fluorescens/putida strain ss-3 (ccm 4430) are described. it shows a weak, but specific and irreversible, antiviral activity on herpes simplex viruses. it also presents some inhibitory activity on different species of yeasts. | 1996 | 8626242 |
| nucleic acid vaccine encoding gd2 protects mice from herpes simplex virus type 2 disease. | nucleic acid vaccinations with plasmids pww65, containing the sequence for herpes simplex type 2 (hsv-2) gd2, and prsvnt, lacking the gd sequence, were studied. groups of mice were immunized with pww65 alone, pww65 plus 1,25-dihydroxyvitamin-d3 (d3), or prsvnt. clinical disease (vaginitis), serum and vaginal washing antibody levels, and vaginal washing virus titers were measured intravaginal hsv-2 challenge. no animals (0/10) in the pww65 + d3 group, 6/10 animals in the pww65 group, and 10/10 an ... | 1996 | 8627014 |
| downstream regulatory elements increase acute and latent herpes simplex virus type 2 latency-associated transcript expression but do not influence recurrence phenotype or establishment of latency. | the role of putative promoter or activator sequences downstream of the herpes simplex virus type 2 latency-associated transcript (lat) promoter and upstream of the lat intron was investigated in vivo by constructing and evaluating mutant viruses with deletions in this region. the deletion of lat promoter sequences upstream of the primary lat transcript reduced levels of lat expression during productive infections, compared with the lat expression level of wild-type virus, and abolished lat expre ... | 1996 | 8627672 |
| locations of herpes simplex virus type 2 glycoprotein b epitopes recognized by human serum immunoglobulin g antibodies. | herpes simplex virus type 2 (hsv-2) glycoprotein b (gb-2) gene segments were expressed as recombinant proteins in escherichia coli. gb-2 recombinant proteins were reacted with human serum immunoglobulin g (igg) antibodies in western immunoblot assays. initially, samples were tested for the presence of hsv-1-specific antibodies and hsv-2-specific antibodies by using hsv-infected cell lysates as antigen targets in western blot assays. serum samples that contained hsv-2-specific igg (n = 58), hsv-1 ... | 1996 | 8627770 |
| primary and secondary syphilis lesions contain mrna for th1 cytokines. | phagocytosis of treponema pallidum by cytokine-activated macrophages aids bacterial clearance and lesion resolution in early syphilis. to investigate the cytokine profiles of cells infiltrating primary and secondary syphilis lesions, reverse transcription and polymerase chain reaction (rt-pcr) were used to detect cytokine mrna in 13 lesion biopsies. both primary and secondary lesions contained mrna encoding interleukin (il)-2, interferon-gamma (ifn-gamma), il-12p40, and il-10. in contrast to a l ... | 1996 | 8568320 |
| synthesis and antiviral activity of 2'-deoxy-4'-thio purine nucleosides. | a series of 2'-deoxy-4'-thioribo purine nucleosides was prepared by trans-n-deoxyribosylase-catalyzed reaction of 2'-deoxy-4'-thiouridine with a variety of purine bases. this synthetic procedure is an improvement over methods previously used to prepare purine 4'-thio nucleosides. the compounds were tested against hepatitis b virus (hbv), human cytomegalovirus (hcmv), herpes simplex virus (hsv-1 and hsv-2), varicella zoster virus (vzv), and human immunodeficiency virus (hiv-1). cytotoxicity was d ... | 1996 | 8558524 |
| the product of a 1.9-kb mrna which overlaps the hsv-1 alkaline nuclease gene (ul12) cannot relieve the growth defects of a null mutant. | alkaline nuclease, a relatively abundant viral phosphoprotein in herpes simplex virus type 1 (hsv-1)- or hsv-2-infected cells, is encoded by a 2.3-kb mrna (r. h. costa, k. g. draper, l. banks, k. l. powell, g. cohen, r. eisenberg, and e. k. wagner, 1983. j. virol. 48, 591-603). this mrna is a member of a family of five unspliced 3'-coterminal messages. costa et al. proposed that another member of this family of mrnas (1.9-kb) may encode an n-terminally truncated protein which shares its carboxy- ... | 1996 | 8560762 |
| synthesis and anti-herpes virus activity of 2'-deoxy-4'-thiopyrimidine nucleosides. | a series of 5-substituted 2'-deoxy-4'-thiopyrimidine nucleosides was synthesized and evaluated as potential antiviral agents. a number of analogues such as 2'-deoxy-5-propyl-4'-thiouridine (3ii), 2'-deoxy-5-isopropyl-4'-thiouridine (3iii), 5-cyclopropyl-2'-deoxy-4'-thiouridine (3iv), 2'-deoxy-4'-thio-5-vinyluridine (3viii), and 5-(2-chloroethyl)-2'-deoxy-4'-thiouridine (3xx) were found to be highly active against herpes simplex virus type-1 (hsv-1) and varicella zoster virus (vzv) in vitro with ... | 1996 | 8576922 |
| douching and sexually transmitted diseases in pregnant women in surabaya, indonesia. | our purpose was to investigate the association between douching (douching agents and timing) and sexually transmitted disease. | 1996 | 8571993 |
| serologically diagnosed infection with human papillomavirus type 16 and risk for subsequent development of cervical carcinoma: nested case-control study. | to study human papillomavirus type 16 in the aetiology of cervical carcinoma. | 1996 | 8595281 |
| slow release devices: std protection at last? | 1996 | 9630916 | |
| controlled release of antibodies for long-term topical passive immunoprotection of female mice against genital herpes. | current methods for sexually transmitted diseases (std) prophylaxis, which can be disruptive and inconvenient, must be used before each act of sexual intercourse, so a method that provides protection over the course of many acts is desirable. we used a mouse model of vaginally-transmitted herpes simplex virus 2 (hsv-2) infection to test polymeric controlled-release devices for sustained passive immunoprotection. vaginal disks were prepared by dispersing a monoclonal antibody to hsv-2 (iii-174) w ... | 1996 | 9630921 |
| [synthesis of optically active (r)- and (s)-tai-ding-an(tda) and their anti-hsv activity evaluation]. | tai-ding-an (3-phthalimido-2-oxo-n-butyraldehyde bisthiosemicarbazone, tda) is an antiviral drug first synthesized in this institute. in order to clarify the difference between the two enantiomeric isomers of tda, (r)- and (s)-tda were synthesized from (r)- and (s)-alanine, respectively, via the following steps: fusing with phthalic anhydride gave 2-phthalimido alanine(2a or 2b). the resulting acid was reacted with thionyl chloride to offer the corresponding acid chloride(3a or 3b), which was tr ... | 1996 | 9863253 |
| [synthesis of adenine derivatives and their activities against herpes virus in vitro]. | a series of 9-(n4-substituted acetaldehyde thiosemicarbazone) adenines were synthesized and evaluated for antiherpes virus activity. compounds 4a-l were prepared by condensation of 9-(acetaldehyde) adenine(6) and the corresponding n4-substituted thiosemicarbazides (10). the antiviral effects of all compounds 4a-l were tested in vitro in primary rabbit kidney cell cultures infected with herpes simplex virus type 1 (hsv-1) and varicella-herpes zoster virus (vzv), and in primary human embryo cell c ... | 1996 | 9863254 |
| [antigenic and genomic characterization of herpes simplex virus isolated from double genital infections]. | two herpes simplex viral serotypes, hsv type 1 and hsv type 2 may cause genital herpes. the aim of this study was to perform a genetical analysis and characterization of virus isolated from four patients with double genital herpetic infections. in eleven viral isolates, the cytopathic effect in vero cells was studied, the antigenic type was determined using monoclonal antibodies and genomic analysis was performed with eco ri, hind iii and bgl ii enzymes. five viral isolates generated a diffuse a ... | 1996 | 9213883 |
| valaciclovir. a review of its antiviral activity, pharmacokinetic properties and therapeutic efficacy in herpesvirus infections. | valaciclovir, the l-valyl ester of aciclovir (acyclovir), is an oral prodrug that undergoes rapid and extensive first-pass metabolism to yield aciclovir and the essential amino acid l-valine. aciclovir, the active antiviral component of valaciclovir, shows good in vitro activity against the herpesviruses herpes simplex virus (hsv)-1, hsv-2 and varicella zoster virus. the bioavailability of aciclovir from oral valaciclovir is considerably greater than that achieved after oral aciclovir administra ... | 1996 | 9118821 |
| herpes simplex virus and pregnancy. | although herpes simplex virus (hsv) infections are common in pregnant women, they are rarely serious. the complication, however, is viral transmission to the infant and morbidity and mortality in neonatal herpes remains considerable, in spite of the availability of antiviral therapy. early recognition and treatment of the infected child is of the utmost importance to inhibit viral replication and thus limit the severity of the disease. moreover, prevention is the ultimate challenge. unfortunatel ... | 1996 | 9163018 |
| herpes simplex encephalitis. | herpes simplex encephalitis (hse) is a life-threatening condition with high mortality as well as significant morbidity in survivors. in most cases herpes simplex virus type 1 (hsv-1) is responsible for the diseases, however, the type 2 virus (hsv-2) is involved in 4-6% of cases. primary hsv infection is identified in only one-third of patients with hse. the majority of cases are recorded in adults with recurrent hsv infection who are already seropositive for hsv at the onset of symptoms, but onl ... | 1996 | 9163027 |
| etiology of acute non-a, b, c hepatitis in thai patients: preliminary study. | to better characterize the etiology of acute non-a, b, c hepatitis, 24 sera from 50 acute hepatitis without acute markers for hepatitis a, b, and c were examined for acute markers for the hepatitis e virus (hev), cytomegalovirus (cmv), herpes simplex virus type 2 (hsv-2), and epstein-barr virus. immunoglobulin m (igm) specific for hev, hsv-2, and cmv was detected using elisa and total ig specific to ebv was determined by standard indirect immunofluorescence. igm to cmv was not observed in sera f ... | 1996 | 9253895 |
| hand trauma and herpes infection. | a herpes simplex type 2 infection of the hand after injury is described in a 26-year-old man. the management and implications of such an unusual hand infection are discussed. | 1996 | 9230963 |
| suppression of subclinical shedding of herpes simplex virus type 2 with acyclovir. | to assess the effect of the antiviral drug acyclovir on the frequency of subclinical shedding of herpes simplex virus (hsv) in the genital tract. | 1996 | 7503497 |
| medical microbiology | in nature, herpesviruses infect both vertebrate and non-vertebrate species, and over a hundred have been at least partially characterized. only eight of these have been isolated routinely from humans and are discussed here. they are known as the human herpesviruses and are herpes simplex virus type 1, herpes simplex virus type 2, varicella-zoster virus, cytomegalovirus, epstein-barr virus, human herpesvirus 6, human ... | 1996 | 21413307 |
| sexually-transmitted disease (std) markers in multitransfused children in relation to human immunodeficiency virus type-1 (hiv-1) infection: impact of std markers in blood donors. | association of serological markers for various sexually transmitted diseases (stds) with human immunodeficiency virus type 1 (hiv-1) infection among heterosexually promiscuous blood donors is well recognized. however, possibility of similar association among children receiving transfusion from these donors has not been adequately explored. study on the association of some std markers with hiv-1 infection in multitransfused (mt) children showed that both hiv-1 positive and hiv-1 negative groups o ... | 1997 | 9231641 |
| viral regulation of cd95 expression and apoptosis in t lymphocytes. | t lymphocyte apoptosis has been observed in vivo in association with viral infections. one mechanism known to mediate t cell apoptosis is the cd95 (fas/apo-1) ag apoptotic pathway. cd95 is a cell surface protein that is expressed on activated t cells and is capable of relaying an intracellular apoptotic signal upon binding with cd95 ligand. cd95-dependent apoptosis has been shown to be involved in the homeostatic control of peripheral t cell numbers; however, the functional significance of the c ... | 1997 | 9233613 |
| the prophylactic effect of immunization with dna encoding herpes simplex virus glycoproteins on hsv-induced disease in guinea pigs. | plasmid expression vectors encoding herpes simplex virus type 2 (hsv-2) glycoproteins b (gb) or d (gd) were constructed and tested for their ability to immunize guinea pigs against genital hsv infection. immunization with a plasmid expressing the aminoterminal 707 amino acids (aa) of gb induced humoral immune responses detected by elisa and virus neutralization. when challenged by vaginal infection, immunized animals were partially protected from genital herpes, exhibiting significantly reduced ... | 1997 | 9234533 |
| sex, drugs, and infections among youth. parenterally and sexually transmitted diseases in a high-risk neighborhood. | to determine the extent to which youth who reside in households in a neighborhood with large numbers of drug injectors 1) are infected with parenterally or sexually transmitted agents, and 2) engage in high-risk behaviors. | 1997 | 9243738 |
| evaluation of a non-isotopic polymerase chain reaction assay for detection in clinical specimens of herpes simplex virus type 2 dna. canadian women's hiv study group. | pcr assays for detection of herpes simplex virus dna sequences in clinical specimens are more sensitive than cell culture. | 1997 | 9248658 |
| detection and differentiation of primate alpha-herpesviruses by pcr. | a rapid method for detection and differentiation of 5 primate alpha-herpesviruses (human herpes simplex virus types 1 and 2 [hsv1, hsv2], green monkey simian agent 8, baboon herpesvirus 2 [hvp2], and macaque b virus [bv]) was developed utilizing the polymerase chain reaction (pcr). pcr primers were located in conserved regions of the gene encoding the glycoprotein b, which flanks an intervening region that is highly divergent among the 5 viruses. amplified pcr products from the 5 viruses were re ... | 1997 | 9249159 |
| protective immunity against hsv-2 in the mouse vagina. | herpes simplex virus type 2 (hsv-2) is a sexually transmitted pathogen that infects the genital tract. the high prevalence of hsv-2 in humans underscores the need to develop an effective vaccine. efforts to develop vaccines to protect women against this and other sexually transmitted pathogens would be facilitated by a better understanding of the immune mechanisms that protect the female reproductive tract against infections in animal models. such information would be invaluable in developing va ... | 1997 | 9430740 |
| tests of vaginal microbicides in the mouse genital herpes model. | microbicide candidates were selected that have demonstrated activity against sperm or sexually transmitted disease pathogens in vitro, and the efficacy of these agents for preventing vaginal transmission of genital herpes infection was evaluated in the progestin-treated mouse. each agent was delivered to the vaginas of mice approximately 20 sec prior to delivering a highly infectious herpes simplex virus-2 inoculum. the following agents provided significant protection: anti-hsv monoclonal antibo ... | 1997 | 9437563 |
| herpes simplex virus. | cns infections caused by human herpesvirus 1 and human herpesvirus 2-herpes simplex virus type 1 and herpes simplex virus type 2--are reviewed. the major diseases associated with these viruses are meningitis and encephalitis. two forms of encephalitis are known, neonatal encephalitis and encephalitis occurring after the neonatal period. both diseases are associated with high mortality and morbidity and require prompt diagnosis and aggressive antiviral chemotherapy. methods for the specific diagn ... | 1997 | 9450224 |
| [herpetic keratitis: clinical-virological correlation]. | herpetic keratitis is the main infectious cause of corneal opacity. the existence of effective antiviral agents underscores the need of an early diagnosis. | 1997 | 9515284 |
| experimental myelitis caused by herpes simplex virus type 2 in c57bl/6n and balb/cn mice. | intraperitoneal and intracranial inoculation of herpes simplex virus type 2 (hsv 2) into balb/cn and c57bl/6n mice was carried out to induce experimental myelitis. the myelitis was clearly observed in c57bl/6n mice following intraperitoneal inoculation. within 24 hours before death, the mice showed urinary and rectal incontinence and paraplegia of the hind legs. randomly distributed, severe necrosis was demonstrated in the spinal cord, mainly at the lower cord. in balb/cn mice the clinical sympt ... | 1997 | 9516872 |
| a novel method to assay herpes simplex virus neutralizing antibodies using bhkicp6lacz-5 (elvis) cells. | a novel method for determining neutralizing serum antibody titers to herpes simplex virus type 2 (hsv-2) was developed based on reduction of infectivity in bhkicp6lacz-5 (elvis) cells; baby hamster kidney (bhk) cells that have been genetically engineered to contain the escherichia coli lacz gene under the control of an inducible herpes simplex virus type 1 (hsv-1) promoter. the test has a semiautomated, colorimetric readout resulting in rapid, objective readings of infectivity reduction. extent ... | 1997 | 9473152 |
| evaluation of antioxidant healing formulations in topical therapy of experimental cutaneous and genital herpes simplex virus infections. | a genital hsv-2 infection of guinea pigs and a cutaneous hsv-1 infection in mice were used to examine the ability of antioxidant components crt1 to reduce lesion development, duration, and severity. crt is a patented antioxidant formulation developed by warner-lambert worldwide consumer healthcare r. and d. crt contains three components that work synergistically: vitamin e, sodium pyruvate and membrane stabilizing fatty acids. the ms strain of hsv-2 was utilized for intravaginal inoculation of g ... | 1997 | 9477116 |
| genital herpes: epidemiology and pathophysiology. update and new perspectives. | genital herpes simplex virus (hsv) is a sexually transmitted disease (std) which affects millions of people worldwide and is mainly due to hsv type 2. seroprevalence rates as high as 60-90% have been reported in developing countries. in developed countries, 20% of the general population is hsv2 seropositive. recent epidemiological surveys employing type-specific antibody assays show that the prevalence of hsv-2 infections is rising at an alarming rate. also, the epidemiology is changing with an ... | 1997 | 9538399 |
| an in vitro assessment of the antineoplastic potential of 2h-1,3-oxazine-2,6(3h)-dione (3-oxauracil), a novel pyrimidine. | the pyrimidine (uracil) analogue 3-oxauracil (ou) previously had been shown to completely inhibit the growth of e. coli b and decrease by 96% the replication of herpes simplex virus type 2 when present in the culture fluid at a concentration of 10(2) microm. limited in vivo studies in mice demonstrated antiviral effects without significant toxicity when given i.p. daily for two weeks at a concentration of 3.23 mg/kg. however, the antineoplastic properties of ou were unknown. we assessed the abil ... | 1997 | 9547671 |
| t-lymphocytopaenia, opportunistic infections and pathological findings in ghanaian aids patients and their sexual partners. | ninety-nine patients at center for disease control (cdc) clinical stage iv were studied. twelve (12.12%) of these patients turned out to be hiv seronegative. ten out of the 12 hiv negative patients were immunocompetent whereas the other two had proportional decreases in both cd4+ and cd8+ t-lymphocytes. hiv-1, hiv-2, and dual infection, were detected in 51.5%, 2%, and 22.2% respectively of clinical aids patients. the other 12.12% of clinical aids patients were indeterminate for hiv antibodies. a ... | 1997 | 9557423 |
| advantage of polymerase chain reaction in the diagnosis of herpes simplex encephalitis: presentation of 5 atypical cases. | four case of herpes encephalitis (hsve) are described. the diagnosis was established by polymerase chain reaction (pcr) assay of cerebrospinal fluid (csf). these reports illustrate different situations in the clinical management of this disease. pcr was considered useful in confirming the hsve diagnosis in 3 atypical cases, and in the differentiation between virologic failure and postinfectious encephalitis in a patient with recurrence of symptoms. a case with typical hsve clinical findings is a ... | 1997 | 9255880 |