Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| coinfection of the central nervous system by cytomegalovirus and herpes simplex virus type 1 or 2 in aids patients: autopsy study on 82 cases by immunohistochemistry and polymerase chain reaction. | we evaluated the frequency and histopathological features of concomitant infections of the central nervous system (cns) with cytomegalovirus (cmv) and herpes simplex viruses type 1 or 2 (hsv1/2) in a large series of patients who had died from aids. eighty-two autopsy cases with a histological diagnosis of cmv necrotizing encephalitis were examined retrospectively. cmv and hsv1/2 were detected by immunohistochemistry (ihc) with poly- and monoclonal antibodies and by nested polymerase chain reacti ... | 1996 | 8891073 |
| relationship of sexual mixing across age and ethnic groups to herpes simplex virus-2 among unmarried heterosexual adults with multiple sexual partners. | sexual mixing is important to understanding how sexually transmitted diseases (stds) spread in the general population, and, identifying people who mix across social groups aids hiv-std prevention. the authors examined (a) the extent to which people have sexual partners from other sexual networks (disassortative mixing) in a probability sample of unmarried heterosexual adults reporting multiple sexual partners (n = 545) and (b) the relationship between mixing and herpes simplex virus-2 (hsv-2). a ... | 1996 | 8891715 |
| induction of fos protein expression in spinal cord neurons by herpes simplex virus infections in the mouse. | after the inoculation of a highly attenuated strain of herpes simplex virus (hsv) type 1 into the mouse hind-paw planter skin, a few neurons and glial cells were viral antigen-positive in the dorsal root ganglia (drgs) and anterior horn of l4 and l5 segments of the lumbar spinal cord. fos-containing nuclei, however, were not found in the drgs or the lumbar cord segments. after the inoculation of a highly neuroinvasive strain of hsv type 2, many virus-positive neurons and glial cells were detecte ... | 1996 | 8892392 |
| disulfide bonds of herpes simplex virus type 2 glycoprotein gb. | glycoprotein b (gb) is the most highly conserved envelope glycoprotein of herpesviruses. the gb protein is required for virus infectivity and cell penetration. recombinant forms of gb being used for the development of subunit vaccines are able to induce virus-neutralizing antibodies and protective efficacy in animal models. to gain structural information about the protein, we have determined the location of the disulfide bonds of a 696-amino-acid residue truncated, recombinant form of herpes sim ... | 1996 | 8892856 |
| high frequency of cd8+ cytotoxic t-lymphocyte precursors specific for herpes simplex viruses in persons with genital herpes. | herpes simplex virus (hsv)-specific cd8+ cytotoxic t lymphocytes (ctl) have rarely been detected in humans, presumably because of virus-induced mechanisms that downregulate major histocompatibility complex class i expression. we have developed a method that has allowed us to consistently demonstrate hsv-specific cd8+ precursor ctl (pctl) from hsv type 1- and 2-seropositive persons. major histocompatibility complex-restricted hsv-specific cd8+ pctl were found in 10 consecutively tested hsv type 1 ... | 1996 | 8892947 |
| chronic meningitis due to herpes simplex virus in an immunocompetent host. | herpes simplex virus (hsv) typically causes mucocutaneous disease, encephalitis, and acute men ingitis. there have been no previous reports of chronic meningitis due to this virus. a case of chronic meningitis due to herpes simplex virus type 2 (hsv-2) in a previously healthy 35-year-old woman whose predominant symptoms were headache and meningism without fever is described. analysis of cerebrospinal fluid (csf) revealed a lymphocytic pleocytosis, elevated protein, and hypoglycorrhachia. the dia ... | 1996 | 8894573 |
| t cell recognition and cytokine production elicited by common and type-specific glycoproteins of herpes simplex virus type 1 and type 2. | t cell recognition of common and type-specific herpes simplex virus (hsv) glycoproteins was measured in 72 subjects. t cells were stimulated with whole hsv-2 antigen and glycoproteins gb2, gd2, and gg2. t cell proliferation in response to hsv-2 antigen and gg2 was significantly higher in subjects with hsv-2 infection than in those with hsv-1 infection only; responses to gb2 and gd2 were the same. t helper (th) type 1 and th2 cytokine production in response to whole hsv-2 antigen, gb2, and gd2 wa ... | 1996 | 8896488 |
| ex vivo protocol for testing virus survival on human skin: experiments with herpesvirus 2. | we report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. at the physiological skin temperature of 32 degrees c, the half-life of the virus on skin was 1.44 h while on metal disks it was 0.36 h. even at ambient temperature (22 degrees c), the virus lost infectivity faster (half-life = 0.96 h) on metal disks ... | 1996 | 8900020 |
| clinicopathologic correlations in acute retinal necrosis caused by herpes simplex virus type 2. | the acute retinal necrosis syndrome is a rapidly progressive and potentially devastating disease. a case of acute retinal necrosis developed in an immunocompetent man, presumably due to the stress, trauma, or immunomodulation related to a craniotomy for a parasellar craniopharyngioma. vitrectomy and endoretinal biopsy were performed. polymerase chain reaction studies of the vitreous revealed herpes simplex virus type 2 as the cause, which has not been previously well documented. results of cereb ... | 1996 | 8906037 |
| detection of herpes simplex virus type 1 by an in situ polymerase chain reaction technique. | the purpose of our study was to develop a method for detecting herpes simplex virus (hsv) dna that combined the high sensitivity of the polymerase chain reaction (pcr) with the precise anatomical localization provided by in situ hybridization (ish). we used in situ pcr (ispcr), ish, and standard pcr methods to determine the proportion of vero cells carrying hsv-1-specific dna before and after 1, 2, and 4 h of infection with hsv-1 or with hsv-2. uninfected vero cells and vero cells infected with ... | 1996 | 8907381 |
| herpes simplex virus type 2 in brazil: seroepidemiologic survey. | the incidence of genital herpes is increasing worldwide. herpes simplex virus type 2 (hsv-2) is the most common cause of genital ulcerations among the sexually transmitted diseases (stds). in order to investigate the sexual transmission of genital herpes infection in brazil, the prevalence of specific antibodies to hsv-2, in populations of high and low risk for stds was evaluated. | 1996 | 8915732 |
| patient recognition of recrudescent herpes labialis: a clinical and virological assessment. | the purpose of this study was to ascertain how accurate the general public was at diagnosing the condition of recrudescent herpes labialis. | 1996 | 8916645 |
| topically applied human recombinant monoclonal igg1 antibody and its fab and f(ab')2 fragments protect mice from vaginal transmission of hsv-2. | a recombinant human anti-herpes simplex virus monoclonal igg1, antibody and the corresponding fab and f(ab')2 fragments were tested for efficacy in preventing vaginal transmission of hsv-2 infection in a well-established mouse model for genital herpes. igg1, fab, and f(ab')2 were approximately equally protective; vaginal delivery of 1-5 ng provided approximately 50% protection, and vaginal delivery of 400 ng completely protected mice from genital herpes infection (p < 0.001). these results sugge ... | 1996 | 8918548 |
| seroprevalence of and risk factors for antibodies to herpes simplex viruses, hepatitis b, and hepatitis c among southwestern hispanic and non-hispanic white women. | few published data describe the seroprevalence of antibodies to herpes viruses and hepatitis viruses among southwestern minority women. | 1996 | 8919741 |
| replication of herpes simplex virus type 1 and 2 in the medulla of the adrenal gland after vaginal infection of mice. | after vaginal infections of mice with neuroinvasive strains of herpes simplex virus type 1 and 2 (hsv-1, hsv-2) virus replicates in the epithelium of the vagina, in the paravaginal ganglia, in the spinal cord and finally in the brain and in the adrenal glands. however, viral antigens could be demonstrated only in the medulla of the adrenal glands but not in the cortex, as assessed by immunohistochemistry (ihc). hsv could not be isolated from liver, spleen, uterus, and ovaries. this contrasts to ... | 1996 | 8920831 |
| long-lived cytotoxic t lymphocyte memory in mucosal tissues after mucosal but not systemic immunization. | the induction and maintenance of long-term ctl memory at mucosal surfaces may be a critical component of protection against mucosal pathogens and is one goal towards development of effective mucosal vaccines. in these studies we have functionally evaluated short and long-term ctl memory in systemic and respiratory or genital-associated lymphoid tissues following mucosal or systemic routes of immunization. our results indicate that shortly after immunizing mice with a recombinant adenovirus vecto ... | 1996 | 8920875 |
| [the effect of opisthorchis on the manifestations of herpesvirus type 2 in an experiment and their possible participation in the mechanism of the occurrence of primary liver cancer]. | the impact of opisthorchiasis on the manifestations of herpes simplex viruses type 2 (hsv-2) was studied. the parasites were established to tend to cause the body's hypersensitivity to hsv-2, particularly after repeated invasions and in summer, the process being characterized by urogenital and bile duct lesions coupled with high proliferation rate in their epithelium. opisthorchiasis became a reservoir and an additional source of viral infection. there were also a definite damage to their reprod ... | 1996 | 8926929 |
| [retrospective study of cervical papillomavirus lesions: early herpes simplex virus proteins as markers of risk for progression]. | specific types of human papillomavirus (hpv) are currently implicated as etiologic agent of benign and malignant lesions of the genital tract. previous studies highlighted the presence of early proteins (icsp 11/12) of herpes simplex virus type 2 (hsv-2) mainly in hpv infected lesions of the cervix. this paper reports a retrospective study on the clinical course of benign cervical alterations in a 70 women population followed-up for 24 months. hpv and hsv-2 (late and early) antigens were detecte ... | 1996 | 8927276 |
| herpes simplex virus type 1 encephalitis in acquired immunodeficiency syndrome. | herpes simplex (hsv) infection of the central nervous system is uncommon in aids and usually has an atypical topography. this review is centred around the case of a 49-year-old homosexual patient with aids who died from diffuse encephalopathy. neuropathological examination revealed necrotic and haemorrhagic changes involving both temporal lobes, insulae and cingulate gyri. cowdry type a intranuclear inclusion bodies were abundant but inflammation was minimal. electron microscopy confirmed charac ... | 1996 | 8930949 |
| neonatal herpes simplex virus infection in the british isles. | this study was set up to estimate the incidence of neonatal herpes simplex virus (hsv) infection in the british isles, and to document the outcome of neonatal infection. paediatricians reported cases of neonatal hsv through the active reporting scheme of the british paediatric association surveillance unit. over a 5 1/2 year period (1986-91) 76 infants with neonatal hsv infection were reported, an incidence of recognised infection in the british isles of 1.65/100000 livebirths. twenty-five infan ... | 1996 | 8931058 |
| influence of mercuric chloride on resistance to generalized infection with herpes simplex virus type 2 in mice. | the effect of mercuric chloride on resistance to generalized infection with herpes simplex virus type 2 (hsv-2) in mice was studied. the severity of the infection was evaluated by the amount of infectious virus in the liver. mercury at a single dose of 20 micrograms aggravated the infection, and neither increasing the single dose to 80 micrograms nor giving repeated doses of 20 micrograms further intensified the infection. examination of the course of infection after mercury exposure revealed an ... | 1996 | 8931761 |
| viral etiology of acute childhood encephalitis in beijing diagnosed by analysis of single samples. | to understand the viral etiology of acute childhood encephalitis in beijing. | 1996 | 8933552 |
| seroprevalence of herpes simplex virus type 2 antibodies in new zealand sexual health clinic patients. | to determine the prevalence of hsv-2 antibodies in std clinic patients in auckland and christchurch between august 1991 and august 1992. | 1996 | 8937391 |
| prevalence of herpesviridae and hepatitis b virus dna in the liver of patients with non-a, non-b fulminant hepatic failure. | members of the herpes virus family and hepatitis b virus (hbv) have been implicated as etiologic agents in non-a, non-b (nanb) fulminant hepatic failure (fhf), but the frequency of infection with these agents has not been established using appropriate controls. to examine this issue, we studied 50 nanb fhf patients and 104 liver transplant recipients from north america and europe. hepatic dna was analyzed by polymerase chain reaction (pcr) for evidence of epstein-barr virus (ebv), cytomegaloviru ... | 1996 | 8938162 |
| production of the r2 subunit of ribonucleotide reductase from herpes simplex virus with prokaryotic and eukaryotic expression systems: higher activity of r2 produced by eukaryotic cells related to higher iron-binding capacity. | the r2 subunit of ribonucleotide reductase from herpes simplex virus type 2 was overproduced with prokaryotic and eukaryotic expression systems. the recombinant r2 purified by a two-step procedure exhibited a 3-fold higher activity when produced in eukaryotic cells. precise quantification of the r2 concentration at each step of the purification indicated that the activity was not altered during the purification procedure. moreover, we have observed that the level of r2 expression, in eukaryotic ... | 1996 | 8947477 |
| transgenic expression of ifn-gamma in the eye as a model for studying ocular inflammatory disease | to study the effects of ifn-gamma on intraocular inflammation we used mice with transgenic expression of ifn-gamma in the retina of the eye. these transgenic mice (rhogamma) were challenged intraocularly with soluble and cellular antigens and then studied for development of uveitis and delayed-type hypersensitivity reaction after a second intracutaneous challenge with the same antigen. further experiments tested the influence of ifn-gamma on infection with herpes simplex (hsv) by intraocular vir ... | 1996 | 8954851 |
| twenty years' delay of fellow eye involvement in herpes simplex virus type 2-associated bilateral acute retinal necrosis syndrome. | to describe a case of acute retinal necrosis with concurrent encephalitis and determine the causative virus. the patient had a history of presumed acute retinal necrosis in the left eye at the age of 8 years and recurrent genital herpes. | 1996 | 8956648 |
| co-therapy with suboptimal doses of interleukin-12 and interferon-alpha promotes synergistic protection against herpes virus infection. | 1996 | 8958953 | |
| dna immunization confers protective immunity on mice challenged intravaginally with herpes simplex virus type 2. | the immunogenicity and protective efficacy of a nucleic acid vaccine encoding the herpes simplex virus type 1 (hsv-1) glycoprotein d (gd) gene under the control of the cmv immediate early gene promoter was examined. mice immunized three times by intramuscular injection with the vaccine developed an hsv specific igg but not iga antibody response detectable in both serum and vaginal secretions. in addition, antigen-specific cellular immune responses were detected in splenic lymphocytes isolated fr ... | 1996 | 8961510 |
| expression and function of the equine herpesvirus 1 virion-associated host shutoff homolog. | the ability of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively) to repress host cell protein synthesis early in infection has been studied extensively and found to involve the activities of the ul41 gene product, the virion-associated host shutoff (vhs) protein. to date, ul41 homologs have been identified in the genomes of three other alphaherpesviruses: equine herpesvirus 1 (ehv-1), varicella-zoster virus, and pseudorabies virus, but very little is known about the putative pro ... | 1996 | 8970998 |
| herpes simplex virus type 2 inhibition of fas ligand expression. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) are common human pathogens. in this report we demonstrate the capacity of hsv-2, but not hsv-1, to inhibit the activity and cell surface expression of fas ligand, an important molecule involved in t-cell apoptosis and cell-mediated cytotoxicity. cells infected with hsv-2 retained fas ligand intracellularly instead of expressing it on the cell surface. addition of anti-fas antibodies markedly inhibited hsv-2 viral production, suggesting that th ... | 1996 | 8971002 |
| herpes simplex virus infections. | 1996 | 8973122 | |
| relationship of the strain and the intraocular amount of herpes simplex virus types 1 and 2 in the induction of anterior-chamber-associated immune deviation. | we investigated the induction of anterior-chamber-associated immune deviation (acaid) by herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). two strains of hsv-1 (vr-3 and f) and four strains of hsv-2 (uw-268, 186, sl, and ms) were inactivated with ultraviolet light, and the anterior chamber of balb/c mice was inoculated with a viral strain. immunization against delayed-type hypersensitivity (dth) was done subcutaneously on day 7 after anterior chamber inoculation. on day 10 after dth immuni ... | 1996 | 8979277 |
| synthesis and microbiological evaluations of (n-heteroaryl) arylmethanamines and their schiff bases. | the synthesis as well as the antimicrobial and antiviral activities of new (n-heteroaryl)arylmethanamines and their schiff bases are reported. none of the tested compounds shown activity against herpes simplex virus type 2 and against gram positive and gram negative bacteria. weak or moderate activity on poliovirus sabin type 1, on reverse transcriptase and against cryptococcus neoformans was shown by some of the tested compounds. viceversa several synthesized compounds exhibited a moderate or g ... | 1996 | 8981754 |
| a genetically inactivated herpes simplex virus type 2 (hsv-2) vaccine provides effective protection against primary and recurrent hsv-2 disease. | a glycoprotein h (gh)-deleted herpes simplex virus type 2 (hsv-2) was evaluated as a vaccine for the prevention of hsv-induced disease. this virus, which we term a disc (disabled infectious single cycle) virus, can only complete one replication cycle in normal cells and should thus be safe yet still able to stimulate broad humoral and cell-mediated antiviral immune responses. a gh-deleted hsv-2 virus that has been tested as a vaccine in the guinea pig model of recurrent hsv-2 infection was const ... | 1997 | 8985191 |
| dextran sulfate can act as an artificial receptor to mediate a type-specific herpes simplex virus infection via glycoprotein b. | herpes simplex virus (hsv) adsorption to host cells is mediated, at least in part, by the interaction of viral glycoproteins with cell surface glycosaminoglycans such as heparan sulfate and chondroitin sulfate. to investigate the contribution of various cell surface components in the infection pathway, we isolated a mutant cell line, sog9, which is unable to synthesize glycosaminoglycans (b. w. banfield, y. leduc, l. esford, k. schubert, and f. tufaro, j. virol. 69:3290-3298, 1995). although hsv ... | 1997 | 8985338 |
| infection and immunity. | 1996 | 8990752 | |
| orally administered hsv-specific transfer factor (tf) prevents genital or labial herpes relapses. | forty-four patients suffering from genital (22) and labial (22) herpes were orally treated with hsv-1/2-specific transfer factor (tf). tf was obtained by in vitro replication of a hsv-1/2-specific bovine dialysable lymphocyte extract. treatment was administered bi-weekly the first 2 weeks, and then weekly for 6 months, most patients received 2-3 courses. the total observation period for all patients before treatment was 26,660 days, with 544 relapses, and a relapse index of 61.2, whereas the cum ... | 1996 | 8993760 |
| use of transfer factor for the treatment of recurrent non-bacterial female cystitis (nbrc): a preliminary report. | results of conventional treatment of female non-bacterial recurrent cystitis (nbrc) are discouraging. most patients show an unexpected high incidence of vaginal candidiasis, while their cell mediated immunity to herpes simplex viruses (hsv) and candida antigens seems impaired, and it is known that the persistence of mucocutaneous chronic candidiasis is mainly due to a selective defect of cmi to candida antigens. twenty nine women suffering of nbrc, and in whom previous treatment with antibiotics ... | 1996 | 8993771 |
| specificity and affinity of binding of herpes simplex virus type 2 glycoprotein b to glycosaminoglycans. | herpes simplex virus type 2 (hsv-2) interacts with cell surface glycosaminoglycans during virus attachment. glycoprotein b of hsv-2 can potentially mediate the interaction between the virion and cell surface glycosaminoglycans. to determine the specificity, kinetics, and affinity of these interactions, we used plasmon resonance-based biosensor technology to measure hsv-2 glycoprotein binding to glycosaminoglycans in real time. the recombinant soluble ectodomain of hsv-2 gb (gb2) but not the solu ... | 1997 | 8995662 |
| synthesis and antiviral activity of 6-chloropurine arabinoside and its 2'-deoxy-2'-fluoro derivative. | 6-chloropurine arabinoside (3a) was obtained by treatment of the 2'-o-acetylated congener (2) with ammonia in methanol. the 3',5'-di-o-tritylated riboside (6) was allowed to react with diethylaminosulfur trifluoride (dast) in the presence of pyridine to give the 2'-deoxy-2'-fluoroarabinoside (7), from which 6-chloro-9-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)purine (3b) was obtained. the antiviral effects of 3a and 3b were assayed against several dna and rna viruses. only 3a displayed potent ac ... | 1996 | 8996865 |
| [efficacy of herpetic vaccine, larifan, myelopid and their combinations during herpesvirus infection in irradiated animals]. | killed vaccine against herpes simplex virus (hsv) 1 and 2, interferon inducer larifane, immunostimulant myelopide, and their combinations were sufficiently effective in immunocompromized mice inoculated intraperitoneally with hsv-1 and hsv-2 three days after a single radiation exposure. combinations of the vaccine with myelopide or larifane had a higher protective effect than that of each of these agents alone. all of these drugs and their combinations more rapidly reduced the concentrations of ... | 1996 | 8999686 |
| pleural effusion due to herpes simplex type ii infection in an immunocompromised host. | pleural effusions caused by herpes simplex viruses are rare. we report a case of a young woman with acute lymphocytic leukemia (all) and prolonged neutropenia who developed pleural space infection with herpes simplex type ii virus (hsv ii), as confirmed by cytologic and microbiologic studies. we believe that this is the first report of a pleural effusion caused by hsv ii, and suggest that this virus now be considered in the differential diagnosis of an unexplained exudative pleural effusion, esp ... | 1997 | 9001338 |
| [genital herpes]. | herpes is the first cause of genital ulcers, recurrences are unpredictable and asymptomatic viral excretion is frequent. herpes simplex virus 2 is responsible for genital infection in 80% of cases, but the incidence of herpes simplex virus 1 is rising. new diagnostics methods are developing, batcellular viral culture remains gold standard. oral aciclovir is the standard treatment, and directions for use are strictly codified. in pregnant women a new preventing project is proposed, it does not pr ... | 1996 | 9005519 |
| herpes simplex virus type 2 synergizes with interferon-gamma in the induction of nitric oxide production in mouse macrophages through autocrine secretion of tumour necrosis factor-alpha. | we have analysed the ability of herpes simplex virus type 2 (hsv-2) to induce nitric oxide (no) production in resting balb/c mouse peritoneal macrophages. in most experiments, macrophages produced very small amounts of no upon infection with hsv-2. mock virus preparations did not induce no production, and virus inactivation experiments showed that infectious virus was required. since interferon-gamma (ifn-gamma) is the prototype cytokine that is able to induce significant no production in macrop ... | 1997 | 9010304 |
| efficacy of cafon gel on cutaneous infection with herpes simplex virus (hsv)-2 and acyclovir-resistant hsv in mice. | caffeine is known to inhibit replication of herpes simplex virus (hsv)-1 and the therapeutic efficacy of caffeine (cafon) gel has been shown in a mouse model cutaneously infected with hsv-1. in this study we examined the inhibitory effect of caffeine on infection with hsv-2 and acyclovir-resistant hsv-1 strains, thymidine kinase (tk)-deficient and phosphonoacetic acid (paa)-resistant hsv-1 in vitro and in vivo. caffeine inhibited plaque formation of hsv-2 and acyclovir-resistant hsv-1 strains an ... | 1996 | 9023706 |
| european consensus on viral encephalitis. | 1997 | 9024370 | |
| placental examination in intrauterine coinfection with herpes simplex virus and cytomegalovirus. | intrauterine coinfections have rarely been reported. however, pregnancies exposed to multiple sexually transmitted infectious agents and drugs are likely to occur with increasing frequency and lead to complex pathology in the newborn. often it will be difficult to establish a diagnosis, above all when this has to be done retrospectively. a premature (34 weeks) newborn presented with a complex clinical picture after exposure to multiple infectious and noninfectious teratogens during gestation. im ... | 1996 | 9025891 |
| analysis of resistance mutants of viral polymerases. | 1996 | 9026659 | |
| mechanism of complement inactivation by glycoprotein c of herpes simplex virus. | glycoprotein c (gc) of both herpes simplex virus type 1 (hsv-1) and hsv-2 interacts with complement c3b and protects the virus from complement-mediated neutralization. to study the mechanism by which gc modulates complement activation, we expressed both gc-1 and gc-2 in a baculovirus expression system. baculovirus recombinants containing gc genes spanning the entire gc-1 sequence (gc-1-tmr) or only the extracellular domain(s) of gc-1, gc-2, or a deletion mutant of gc-1 lacking residues 33 throug ... | 1997 | 9029114 |
| viral lesions of the mouth in hiv-infected patients. | viral lesions of the mouth in patients with hiv infection are common and these diseases any be a marker for hiv and disease progression. we review the spectrum of oral viral manifestations and discuss treatment modalities. the most common epstein-barr virus (ebv)-induced disorder in hiv-infected patients is oral hairy leukoplakia. ebv-related oral b-cell and t-cell lymphoma in aids patients has been described repeatedly. herpes virus type 1 and rarely type 2 may lead to painful and resistant ora ... | 1997 | 9031782 |
| evaluation of a quantitative competitive pcr assay for measuring herpes simplex virus dna content in genital tract secretions. | previous studies have shown an association between the approximate titer of herpes simplex virus (hsv) dna in clinical specimens and the ability to isolate hsv from genital secretions. to control for variance in amplification conditions, we developed a competitive quantitative pcr (qc pcr) for the detection of hsv dna. the assay accurately measured from 10 to 10(6) copies of hsv dna. we compared the qc pcr with our previous semiquantitative detection method and found concordance for 61 of 63 pos ... | 1997 | 9041386 |
| asymptomatic perianal shedding of herpes simplex virus in patients with acquired immunodeficiency syndrome. | to determine the frequency of asymptomatic perianal shedding of herpes simplex virus (hsv) in adult patients with acquired immunodeficiency syndrome (aids). | 1997 | 9041831 |
| novel 3'-c/n-substituted 2',3'-beta-d-dideoxynucleosides as potential chemotherapeutic agents. 1. thymidine derivatives: synthesis, structure, and broad spectrum antiviral properties. | a synthetic scheme for the 3'-oxime derivatives 3e, 5e, 5z, 7e and 7z of 1-(2,3-dideoxy-beta-d-glycero-pentofuranosyl)thymine and for 1-(2,3-dideoxy-3-nitro-beta-d-erythro-pentofuranosyl)-thymine (10) has been developed starting from appropriately 5'-protected 3'-ketothymidine. x-ray analysis showed that 3'-n-hydroxyimino 3e and 3'-n-methoxyimino 5z derivatives have close molecular conformations: anti about the n1-c1' bond, and gauche+ about the c4'-c5' exocyclic bond. their sugar conformations ... | 1997 | 9046339 |
| nucleotide sequence of the herpes simplex virus type 2 gene encoding the protease and capsid protein icp35. | the complete nucleotide sequence of the gene encoding the protease and icp35 capsid protein of herpes simplex virus (hsv) type 2 strain g has been determined. the gene consisits of a 1908 bp open reading frame capable of encoding 636 amino acids. comparative nucleotide sequence analysis of the gene with published hsv-1 sequences revealed that the hsv-2 nucleic acid segment most likely encodes two distinct proteins. the first protein is the hsv-2 protease, which is required for the assembly of th ... | 1995 | 9049360 |
| single amino acid substitutions in the glycoprotein b carboxy terminus influence the fusion from without property of herpes simplex virus type 1. | syncytial mutations of herpes simplex virus type 1 (hsv-1) strains ang, ang path, hfem, tsb5 and hszp cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein b (gb), within the syn 3 locus. these strains are so far the only ones which show the phenotype 'fusion from without' (ffwo): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. in this report we detected, using the recombinants ... | 1995 | 9049391 |
| antigenic differences between hsv-1 and hsv-2 glycoproteins and their importance for type-specific serology. | the ability to discriminate between herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) infections by serological means is of increasing importance for clinical virological diagnostics. hsv type-specific serology has a number of applications, e.g. to guide duration and dosage of antiviral therapy, to allow for stringent epidemiological analyses, to evaluate efficacy of hsv vaccine candidates, and to help the clinician during counseling of couples where one has genital herpes. the genomes of the ... | 1996 | 9058170 |
| antigenic structure of soluble herpes simplex virus (hsv) glycoprotein d correlates with inhibition of hsv infection. | soluble forms of herpes simplex virus (hsv) glycoprotein d (gd) block viral penetration. likewise, most hsv strains are sensitive to gd-mediated interference by cells expressing gd. the mechanism of both forms of gd-mediated inhibition is thought to be at the receptor level. we analyzed the ability of different forms of soluble, truncated gd (gdt) to inhibit infection by different strains of hsv-1 and hsv-2. strains that were resistant to gd-mediated interference were also resistant to inhibitio ... | 1997 | 9060653 |
| frequent genital herpes simplex virus 2 shedding in immunocompetent women. effect of acyclovir treatment. | reactivation of herpes simplex virus type 2 (hsv-2) occurs intermittently as perceived clinically and by viral culture. we performed a series of studies to evaluate the frequency and pattern of hsv-2 reactivation using both viral isolation and hsv pcr assay. daily samples of genital secretions were obtained from 27 hsv-2 seropositive women; a subset of subjects obtained samples while receiving oral acyclovir 400 mg po twice a day. hsv dna was detected in genital swab specimens on 28% of 1,410 d ... | 1997 | 9062368 |
| herpes simplex virus type 2 detection by culture and polymerase chain reaction and relationship to genital symptoms and cervical antibody status during the third trimester of pregnancy. | our goal was to define the frequency of asymptomatic herpes simplex virus type 2 shedding by culture and polymerase chain reaction and to correlate our findings with cervical anti-herpes simplex virus type 2 immunoglobulin a production. | 1997 | 9065196 |
| hearing loss and herpes simplex. | a survey to identify the aetiology of hearing impairment among saudi children was carried out. children were divided into two groups according to presence or absence of laboratory evidence of herpes simplex virus infection 'at risk' and 'not at risk'. serological tests for herpes simplex virus infection were performed on 1054 children. we found positive igm antibody against herpes simplex virus, type 1 (hsv1) in the blood of 82 of the 1054 children (8 per cent), and positive igm antibody against ... | 1997 | 9078824 |
| current clinical issues in the management of herpes simplex virus infections in patients with hiv. | a recent study done in baltimore showed hsv-2 seroprevalence of 81% among 64 hiv-positive homosexual or bisexual men. | 1997 | 9094453 |
| hepatitis b markers in men seeking human immunodeficiency virus antibody testing in mexico city. | to estimate the prevalence and associated risk factors of hepatitis b virus (hbv) serologic markers in 1,377 men with high-risk sexual behavior attending a center for human immunodeficiency virus (hiv) detection in mexico city. | 1997 | 9101632 |
| [herpes simplex: generalized infection in a newborn]. | most mucose-cutaneous infections can be diagnosed by clinical history with explorative technique. nevertheless the definitive etiologic diagnostic can only be established with the help of the isolation and identification of the causal agent. | 1997 | 9101753 |
| [evaluation of the immune and interferon status of children infected with herpes simplex virus and living in areas with increased radiation background]. | virological and immunological examinations were carried out in 52 children aged 4 to 15 living at the bryansk district territories contaminated with radionuclides after the chernobyl accident. the persistence of herpes simplex virus (hsv) in the blood was detected by indirect immunofluorescence in 90% of children, in 10% it was confirmed by the virus isolation in vitro in a cell culture. specific antibodies to hsv-1 and hsv-2 were detected by enzyme immunoassay in 100% examinees, in 50% their ti ... | 1997 | 9103044 |
| cognitive-behavioral stress management decreases dysphoric mood and herpes simplex virus-type 2 antibody titers in symptomatic hiv-seropositive gay men. | this study tested the effects of a 10-week group cognitive-behavioral stress management (cbsm) intervention on mood and immunologic parameters in hiv-seropositive gay men whose disease had progressed to a symptomatic stage. men were randomized to either cbsm or a modified waiting-list control group. the cbsm intervention significantly decreased self-reported dysphoria, anxiety, and total distress. individuals who practiced relaxation more consistently had significantly greater drops in dysphoria ... | 1997 | 9103732 |
| [virus-related neurological disorders complicating in compromised hosts]. | chance of opportunistic infections in hospitals is increasing year by year, because of the steady increase in the number of compromised hosts. opportunistic infections in the nervous system include fungal, protozoal, bacterial and viral infections. cytomegalovirus and herpes virus (hsv1 and hsv2) cause encephalitis, myelitis and radiculoneuritis. varicella-zoster virus (vzv) often causes herpes zoster, which sometimes disseminates when immunodeficiency is severe. vzv also causes encephalitis. pr ... | 1997 | 9103900 |
| genomic polymorphism in herpes simplex virus keratitis. | viruses are the major infectious agents causing conjunctivitis and keratoconjunctivitis. two antigenic types, herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), can be recognized in which numerous genomic variants or genotypes have been described. hsv-1 infection is one of the major causes of blindness. different strains can induce different types of ocular lesions. twelve patients with acute herpetic epithelial keratitis were studied by monoclonal antibodies and restriction enzymes, and ni ... | 1997 | 9106185 |
| testing of some pharmacological characteristics of the autochthonous product acyclovir. | in the present work, the preliminary results of a study regarding the pharmacokinetic properties of the autochthonous synthetic drug acyclovir are shown. the in vitro and in vivo toxicologic test demonstrates a low toxicity of this product, the drug being well tolerated both by the laboratory animal and by the fibroblasts of the human embryo. the specific antiviral action of the product on the multiplication in vitro of the herpes simplex virus types 1 and 2, appreciated by the lowering capacity ... | 1995 | 9106397 |
| seroprevalence of herpes simplex virus types 1 and 2 and cytomegalovirus in adolescents. | we determined the prevalence of herpes simplex virus (hsv) and cytomegalovirus (cmv) antibodies in a cohort of adolescents 12 to 22 years of age in anticipation of the development of vaccines to control hsv and cmv infections. for the overall study population, we found that 62% were seropositive for hsv type 1 (hsv-1), 12% were seropositive for hsv type 2 (hsv-2), and 65% were seropositive for cmv. race was not related to hsv-1 seropositivity, but african-american adolescents were more likely th ... | 1997 | 9114136 |
| valaciclovir. a review of its antiviral activity, pharmacokinetic properties and therapeutic efficacy in herpesvirus infections. | valaciclovir, the l-valyl ester of aciclovir (acyclovir), is an oral prodrug that undergoes rapid and extensive first-pass metabolism to yield aciclovir and the essential amino acid l-valine. aciclovir, the active antiviral component of valaciclovir, shows good in vitro activity against the herpesviruses herpes simplex virus (hsv)-1, hsv-2 and varicella zoster virus. the bioavailability of aciclovir from oral valaciclovir is considerably greater than that achieved after oral aciclovir administra ... | 1996 | 9118821 |
| interferon-gamma enhances resolution of herpes simplex virus type 2 infection of the murine genital tract. | the requirement for interferon-gamma (ifn gamma) in resolution of an hsv-2 vaginal infection and the cellular sources of this cytokine in the vaginal mucosa were assessed. ifn gamma levels in vaginal secretions peaked on days 2 and 5 following hsv-2 inoculation. natural killer (nk) cell depletion greatly diminished the early production of ifn gamma but had no significant effect on the rate of virus clearance. cd4+ t cells were primarily responsible for the second peak of ifn gamma levels and neu ... | 1997 | 9123869 |
| treatment of spontaneously arising retinoblastoma tumors in transgenic mice with an attenuated herpes simplex virus mutant. | the use of viruses to treat tumors has received renewed interest with the availability of genetically defined attenuated mutants. herpes simplex virus (hsv) type 1 in particular has been shown to be effective for tumors of neuronal origin. however, the model systems used for these studies rely on the use of explanted tumor cells in immunodeficient animals. we have used a recently developed transgenic mouse model, wherein mice spontaneously develop retinoblastomas, to determine if a mutant hsv ha ... | 1997 | 9123872 |
| a system for isolation, transport and storage of herpes simplex viruses. | a major difficulty with diagnostic virus isolation concerns the relative thermolability of certain viruses, e.g. herpes simplex virus type 2, which may, therefore, lose infectivity during transport to the laboratory. this study describes a system of virus isolation and transport, which depends on direct inoculation at the bedside or clinic, to a monolayer or suspension of susceptible cells with subsequent incubation for 10 h at approximately 32 degrees c, whereupon the newly synthesised virus be ... | 1997 | 9128856 |
| preferential presentation of herpes simplex virus t-cell antigen by hla dqa1*0501/dqb1*0201 in comparison to hla dqa1*0201/dqb1*0201. | the hla dqa1 locus is polymorphic. haplotypes containing hla dqa1*0501, but not hla dqa1*0201, together with hla dqb1*0201 are associated with grave's disease and celiac sprue. in this report, we demonstrate a functional correlate of dqa1 polymorphism. t cells infiltrating a herpes simplex virus (hsv) lesion from a hla dq 2,7 individual yielded a virus-specific cd4+ clone restricted by dq2. presentation of viral peptide and protein segregated with dqa1 allele, because cell lines bearing dqa1*050 ... | 1997 | 9129979 |
| genital herpes in a primary care clinic. demographic and sexual correlates of herpes simplex type 2 infections. | genital herpes remains one of the most prevalent sexually transmitted diseases (stds). the sexual behavioral correlates of herpes simplex virus type 2 (hsv-2) infection in the general population have not been well characterized. | 1997 | 9132981 |
| herpetic urethritis in male patients in ibadan. | in a study of 151 male patients attending a sexually transmitted disease clinic, 40 (26.4%) had non-gonococcal urethritis. herpes simplex virus was isolated in tissue culture of hep-2 cell line from 10 of the patients with non-gonococcal urethritis, and identified by complement fixation and indirect immunofluorescent anti body tests. of the isolates, 30% were herpes simplex virus type 1 (hsv-1), while 70% were herpes simplex virus types (hsv-2). | 1997 | 9133820 |
| clear detection and typing of herpes simplex virus types 1 and 2 by an indirect elisa assay: comparison with three different combined methods--capture elisa, restriction enzymes, and polymerase chain reaction. | the severity and recurrences of herpes simplex virus (hsv) infection depend on the type of the infectious agent (hsv-1 or hsv-2), which induces the necessity of a nonambiguous detecting typing. the commonly used capture elisa technique has to be often supported by dna analysis to confirm the detection and the typing of hsv viruses in exposed patients. in this report, we describe a rapid and cheap indirect elisa method using anti-hsv monospecific polyclonal antibodies prepared in the laboratory. ... | 1997 | 9138103 |
| herpes simplex blepharoconjunctivitis presenting as complete acquired ankyloblepharon. | a 90-year-old woman was referred to us with a 1-month history of progressively worsening blepharoconjunctivitis. she had a complete acquired ankyloblepharon of the right lids, which resulted in the appearance of a right upper lid abscess on computed tomography. | 1997 | 9143812 |
| the active site of icp47, a herpes simplex virus-encoded inhibitor of the major histocompatibility complex (mhc)-encoded peptide transporter associated with antigen processing (tap), maps to the nh2-terminal 35 residues. | the herpes simplex virus (hsv) immediate early protein icp47 inhibits the transporter associated with antigen processing (tap)-dependent peptide translocation. as a consequence, empty major histocompatibility complex (mhc) class i molecules are retained in the endoplasmic reticulum and recognition of hsv-infected cells by cytotoxic t lymphocytes is abolished. we chemically synthesized full-length icp47 (sicp47) and show that sicp47 inhibits tap-dependent peptide translocation in human cells. its ... | 1997 | 9151894 |
| localization of latency-associated transcripts in the uterovaginal plexus of herpes simplex virus type 1 and 2 latently infected mice. | the vagina and medulla of the adrenal gland of mice vaginally infected with herpes simplex virus (hsv) types 1 and 2 were examined in the latent stage of infection (5 to 51 weeks post-infection). rna in situ hybridization with hsv-1 and -2 latency-associated transcript (lat) rna probes resulted in positively stained neuronal cell nuclei in the uterovaginal plexus, but not in the medulla of the adrenal gland. these organs were chosen because hsv antigens can be detected not only in the vaginal ep ... | 1997 | 9152429 |
| antibody to haemophilus ducreyi among trucking company workers in kenya. | to determine the prevalence, correlates, and incidence of haemophilus ducreyi antibodies, a cohort of east african trucking company employees was evaluated. | 1997 | 9153735 |
| herpes simplex virus and pregnancy. | although herpes simplex virus (hsv) infections are common in pregnant women, they are rarely serious. the complication, however, is viral transmission to the infant and morbidity and mortality in neonatal herpes remains considerable, in spite of the availability of antiviral therapy. early recognition and treatment of the infected child is of the utmost importance to inhibit viral replication and thus limit the severity of the disease. moreover, prevention is the ultimate challenge. unfortunatel ... | 1996 | 9163018 |
| herpes simplex encephalitis. | herpes simplex encephalitis (hse) is a life-threatening condition with high mortality as well as significant morbidity in survivors. in most cases herpes simplex virus type 1 (hsv-1) is responsible for the diseases, however, the type 2 virus (hsv-2) is involved in 4-6% of cases. primary hsv infection is identified in only one-third of patients with hse. the majority of cases are recorded in adults with recurrent hsv infection who are already seropositive for hsv at the onset of symptoms, but onl ... | 1996 | 9163027 |
| detection and typing of herpes simplex viruses by using recombinant immunoglobulin fragments produced in bacteria. | thirty-seven bacterial clones producing human recombinant monoclonal antibody fab fragments (rfabs) reactive to herpes simplex virus (hsv) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an hsv lysate. thirty-four of the hsv-specific rfabs were able to specifically recognize hsv-infected cells in indirect immunofluorescence (if) assays; of these, 25 recognized cells infected by either hsv type 1 (hsv-1) or hs ... | 1997 | 9163470 |
| [an autopsied case doubly infected by mucor mycelia and herpes simplex virus-2 in the remission state after bone marrow transplantation for acute myelogenous leukemia]. | a 36-year-old woman who had been in complete remission after bone marrow transplantation against her leukemia presented with visual disturbance and cerebellar signs. she showed unusual involuntary movement as if she rolled a ball of string, and died two months after the onset of illness. necropsy revealed hemorrhagic and necrotic lesions in the thalamus, basal ganglia, cerebellar dentate nucleus, superior colliculus, midbrain pretectum and red nucleus, and mucor mycelia were numerously found. hs ... | 1997 | 9163758 |
| ap-1 cis-response elements are involved in basal expression and vmw110 transactivation of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10). | the promoter of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) has two ap-1 cis-response elements, respectively located at positions -62 and -94 relative to the transcription start site (wymer et al., 1989. j. virol. 63, 2773-2784). chloramphenicol acetyl transferase (cat) analysis with hybrid constructions of the cat structural gene and the icp10 promoter or its mutants and gel retardation studies were used to examine the role of the ap-1 cis-response elements ... | 1997 | 9168892 |
| genital herpes during pregnancy: inability to distinguish primary and recurrent infections clinically. | to determine if the signs and symptoms of genital herpes in pregnancy accurately identify primary genital herpes infections using serologic testing for final classification. | 1997 | 9170460 |
| distribution of herpes simplex virus types 1 and 2 genomes in human spinal ganglia studied by pcr and in situ hybridization. | clinical data indicate that the recurring herpes simplex virus (hsv) from oro-labial lesions is hsv subtype 1 and that the virus from genital lesions is hsv-2. this suggests that hsv-1 and hsv-2 reside in latent forms in the trigeminal ganglia and sacral ganglia, respectively. however, the distribution of latent hsv-1 and hsv-2 infections in human spinal ganglia has not been fully examined. this report concerns the application of polymerase chain reaction (pcr) and in situ hybridization (ish) to ... | 1997 | 9179758 |
| herpes simplex virus virulence: the functions of the gamma (1)34.5 gene. | 1997 | 9179780 | |
| antiherpetic and anticoagulant properties of carrageenans from the red seaweed gigartina skottsbergii and their cyclized derivatives: correlation between structure and biological activity. | the antiviral activity against herpes simplex virus types 1 and 2 of kappa/l-, partially cyclized mu/v-, and lambda-carrageenans isolated from the red seaweed gigartina skottsbergii and their cyclized derivatives was analyzed. lambda-carrageenans and the partially cyclized mu/v-carrageenan were the most potent inhibitors of herpes viruses (including acyclovir-resistant variants and clinical isolates), with ic50 values lower than 1 microgram ml-1 against both serotypes and selectivity indices hig ... | 1997 | 9184941 |
| construction and characterization of a replication-defective herpes simplex virus 2 icp8 mutant strain and its use in immunization studies in a guinea pig model of genital disease. | a replication-defective mutant of herpes simplex virus 2 (hsv-2) was engineered by replacing the icp8 gene of hsv-2 strain 186 with an icp8-lacz fusion gene from the herpes simplex virus 1 (hsv-1) hd-2 mutant strain. the resulting virus, hsv-2 5blacz, is defective for growth in vero cells but is capable of growth in a cell line that expresses hsv-1 icp8. in vero cells, the mutant virus is defective for dna synthesis but is able to express many viral proteins at levels similar to those of wild-ty ... | 1997 | 9185583 |
| inhibition of human immunodeficiency virus replication by the herpes simplex virus virion host shutoff protein. | the herpes simplex virus (hsv) virion host shutoff gene (vhs) encodes a protein which nonspecifically accelerates the degradation of mrna molecules, leading to inhibition of protein synthesis. this ability to inhibit a critical cellular function suggested that vhs could be used as a suicide gene in certain gene therapy applications. to investigate whether vhs might be useful for treatment of aids, we tested the ability of both hsv type 1 (hsv-1) and hsv-2 vhs to inhibit replication of human immu ... | 1997 | 9188626 |
| herpes virus-specific immune responses in individuals experiencing recurrent genital herpes. | 1997 | 9191322 | |
| herpes simplex virus-specific igm, iga and igg subclass antibody responses in primary and nonprimary genital herpes patients. | to clarify the humoral immunity in herpes simplex virus (hsv) infection, hsv-specific igm, iga and igg subclass antibody responses were studied in patients with genital herpes: 17 primary, 13 recurrent and 6 nonprimary first episode. a total of 181 serum samples serially collected from the patients, 5 per patient until 213 days after the onset of disease (on average), were analyzed by an enzyme-linked immunosorbent assay. igg1, igg3 and iga were detected in all patients with primary and nonprima ... | 1997 | 9194040 |
| fine-resolution analysis of products of intrachromosomal homeologous recombination in mammalian cells. | mouse ltk- cell lines that contained a herpes simplex virus type 1 (hsv-1) thymidine kinase (tk) gene with a 16-bp insertion mutation linked to either a defective hsv-2 tk gene or a hybrid tk sequence comprised of hsv-1 and hsv-2 tk sequences were constructed. hsv-1 and hsv-2 tk genes have 81% nucleotide identity and hence are homeologous. correction of the insertion mutant hsv-1 tk gene via recombination with the hybrid tk sequence required an exchange between homeologous tk sequences, although ... | 1997 | 9199296 |
| monoclonal antibodies to the distinct antigenic sites on glycoproteins c and b and their protective abilities in herpes simplex virus infection. | the relative importance of the humoral immune response to various antigenic sites on the glycoproteins c and b (gc, gb) of herpes simplex virus (hsv) was evaluated in balb/c and dba/2 mice passively immunized with monoclonal antibodies (moabs) and then challenged with lethal dose of infectious virus. eight moabs to three topographically distinct antigenic sites on gc and eight moabs to two distinct antigenic sites on gb were selected. the results indicated that any antigenic site on gc and gb co ... | 1997 | 9199708 |
| antiviral and tumor cell antiproliferative sar studies on tetracyclic eudistomins. ii. | in a search for the minimum pharmacophore of the naturally occurring tetracyclic eudistomins, five structural analogues (4-8) were evaluated for their in vitro antiviral and tumor cell antiproliferative activities. for the synthesis of these derivatives both intra- and intermolecular pictet-spengler reactions have been used. opening of the beta-carboline annulated 7-membered d-ring in 6 and 7 resulted in a complete loss of activity. on the other hand, replacement of either the oxygen atom or the ... | 1997 | 9208105 |
| apoptosis induced in the spinal cord and dorsal root ganglion by infection of herpes simplex virus type 2 in the mouse. | after inoculation of a highly neuro-invasive strain of herpes simplex virus (hsv) type 2 (186) into the mouse hind-paw planter skin, many virus-positive neurons and glial cells were detected in the dorsal root ganglia (drgs) and lumbar spinal cord by immunohistochemistry for hsv-2 antigen. a number of apoptotic cells were also observed in the spinal cord and drgs by the terminal dutp nick-end-labeling (tunel) method. double labeling with the immunohistochemistry for hsv-2 and the tunel method re ... | 1997 | 9209108 |
| [antigenic and genomic characterization of herpes simplex virus isolated from double genital infections]. | two herpes simplex viral serotypes, hsv type 1 and hsv type 2 may cause genital herpes. the aim of this study was to perform a genetical analysis and characterization of virus isolated from four patients with double genital herpetic infections. in eleven viral isolates, the cytopathic effect in vero cells was studied, the antigenic type was determined using monoclonal antibodies and genomic analysis was performed with eco ri, hind iii and bgl ii enzymes. five viral isolates generated a diffuse a ... | 1996 | 9213883 |