Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acute retinal necrosis caused by reactivation of herpes simplex virus type 2. | acute retinal necrosis is a severe form of necrotizing retinitis. acute retinal necrosis has been demonstrated to be caused by varicella-zoster virus and herpes simplex virus type 1. we treated three patients with acute retinal necrosis apparently caused by recrudescence of latent herpes simplex virus type 2. primary viral infection was probably congenital, with documented perinatal herpes simplex virus type 2 infection in two patients. bilateral chorioretinal scars were present in two patients, ... | 1994 | 8053466 |
| synthesis and antiviral activity of three pyrazole analogues of distamycin a. | the synthesis of three new monopyrazole analogues of the antiviral compound distamycin a is reported. suitably protected 4-amino-1-methylpyrrole-2-carboxylic acid and 3-amino-1-methylpyrazole-5-carboxylic acid derivatives were chosen as starting materials. the construction of the trimeric polyamide framework was accomplished by assembly of the monomeric precursors under condensing conditions by analogy with our previous methodology, although with significant improvements in some pivotal steps. a ... | 1994 | 8060726 |
| epidemiology of genital herpes. | new serological methods, based on the detection of type-specific antibodies to herpes simplex virus (hsv) glycoproteins, have allowed a reappraisal of the prevalence of genital herpes. during the 1980s, genital herpes continued to be an infection of epidemic proportions in many developed countries. in the usa, although there was evidence of a decline in the age-specific prevalence of hsv type 1 (hsv-1) infection, the seroprevalence of hsv type 2 (hsv-2) has continued to increase. among those att ... | 1994 | 8063020 |
| undocumented history of maternal genital herpes followed by neonatal herpes meningitis. | 1994 | 8064427 | |
| synthesis of some acylphosphonate thiosemicarbazones and evaluation of their antiviral activity. | reaction of dimethyl acylphosphonates (1) with thiosemicarbazide afforded the corresponding dimethyl acylphosphonate thiosemicarbazones (4). lithium methyl acylphosphonate thiosemicarbazones (5) could be prepared either by monodealkylating compounds 4 by lithium bromide or by reacting lithium methyl acylphosphonates with thiosemicarbazide. dihydrogen acylphosphonate thiosemicarbazones (6) could be obtained by reacting acylphosphonic acids (3) with thiosemicarbazide. the alternative approach to 6 ... | 1994 | 8068818 |
| follow-up of pregnant women at high risk of transmitting herpes simplex virus. | prospects for neonatal herpes transmission were studied in a group of pregnant chilean women at high herpetic risk--including 59 with a history of genital herpes, 11 with a first genital herpes episode during the observed pregnancy, and 16 whose sexual partners had a history of genital herpes. each woman completed a survey questionnaire, provided serologic samples for detection of herpes simplex virus (hsv), and provided periodic samples taken with cotton swabs for hsv isolation. the 86 women wh ... | 1994 | 8069336 |
| epithelial interleukin-11. regulation by cytokines, respiratory syncytial virus, and retinoic acid. | interleukin-11 (il-11) is a pleiotropic cytokine with effects that overlap with il-6. to determine if il-11 is produced by epithelial cells, we determined whether human alveolar a549 cells and airway 9hte cells produce il-11. we also determined whether retinoic acid (ra) altered this il-11 production. unstimulated cells produced low levels of il-11, while il-1, transforming growth factor (tgf-beta 1), and respiratory syncytial virus (rsv) stimulated il-11 protein production and mrna accumulation ... | 1994 | 8071352 |
| synthesis and antiviral activity evaluation of some aminoadamantane derivatives. | the synthesis of some spiro[cyclopropane-1,2'-adamantan]-2-amines and methanamines and some spiro[pyrrolidine-2,2'-adamantanes] is described. the title compounds were evaluated against a wide range of viruses (influenza a, influenza b, parainfluenza 3, rsv, hsv-1, tk- hsv-1, hsv-2, vaccinia, vesicular stomatitis, polio 1, coxsackie b4, sindbis, semliki forest, reo 1, hiv-1, and hiv-2), and some of them (compounds 6b, 6c, 9a, 16a, 16b, and 17) inhibited the cytopathicity of influenza a virus at a ... | 1994 | 8071937 |
| [acute benign lymphocytic meningitis caused by type 2 herpes simplex virus]. | 1994 | 8072966 | |
| detection of human alpha-herpesvirus dna using consensus primers and specific probes. | a simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. this system was based on an amplification step, using primers that bind the dna polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. this method could be used to amplify any human alpha-herpesviruses, and each v ... | 1994 | 8073876 |
| cell type and cell state determine differential in vitro growth of non-neurovirulent icp34.5-negative herpes simplex virus types 1 and 2. | the herpes simplex virus (hsv) gene rl1 encodes the protein icp34.5, which is a specific neurovirulence factor. null mutants in rl1 fail to replicate in the central nervous system of mice and are therefore totally non-neurovirulent. additionally, they fail to replicate in neurons of the peripheral nervous system, although they are capable of establishing and reactivating from a latent infection. as the precise function of icp34.5 in hsv-neuronal interactions is unknown, we have studied the role ... | 1994 | 8077935 |
| [the primary immune response and virus-specific and -nonspecific immune complexes in neonatal cytomegalovirus and herpetic infection]. | 1993 | 8079522 | |
| herpes simplex virus type 2 induced retinal necrosis in balb/c mice. | we injected herpes simplex virus type 2 of ms- or g-strain into the anterior chamber of balb/c mice. in the contralateral eye inflammatory cell infiltration began in the ciliary body; focal retinitis, detected by day 8, led to total destruction of the retina by day 10. contralateral disease was observed in 75% of mice inoculated with 8 x 10(3) pfu herpes simplex virus type 2, but in only 20% of mice receiving 80 pfu herpes simplex virus type 2. still this low concentration, however, produced a s ... | 1994 | 8079627 |
| biological response of recombinant interleukin-7 on herpes simplex virus infection in guinea-pigs. | we investigated the biological activity of human recombinant interleukin-7 (rhil-7) on guinea-pig cells in vitro and in vivo. rhil-7 can stimulate plastic non-adherent cells of guinea-pigs to proliferate in vitro at a degree comparable to that of human cells. administration of rhil-7 to guinea-pigs significantly increased their white blood cell concentration, specifically the lymphocyte population. the effect of rhil-7 on circulating blood cells was dose dependent, in that 14 doses of twice dail ... | 1994 | 8085384 |
| herpes simplex virus and oral mucositis in children with cancer. | the relationship between herpes simplex virus (hsv) and oral mucositis was investigated in children undergoing antineoplastic chemotherapy. hsv culture was performed in 20 children with stomatitis developing after antineoplastic chemotherapy. viral isolates were typed and susceptibility to acyclovir was investigated. the virus was isolated from oral lesions in 10 of 20 children with severe oral mucositis. viral reactivation was the most likely explanation in most cases, since hsv was isolated in ... | 1994 | 8087447 |
| sexually transmitted diseases in hiv-infected persons. | it appears incontestable that there is a link between genital ulcer disease and hiv infection. on the one hand the natural history and response to therapy of syphilis, hsv-2, and chancroid are all modified by the immunosuppressive effects of hiv infection. on the other hand, hiv transmission is probably facilitated by the disruption of the normal epithelial barriers of the genital organs caused by these ulcerative infections. information is somewhat less convincing that a similar association exi ... | 1994 | 8089470 |
| [the production of recombinant polypeptides from the herpes simplex viruses types 1 and 2]. | the genes ie175, ie63, ie68, ie12, ul29 and ul19 of herpes simplex virus types 1 and 2 were cloned. fragments of these genes were cloned into open reading frame expression vector pex1-3. recombinant proteins containing amino acid sequences of icp4, icp27, icp22, icp47, major dna-binding protein and major capsid protein were expressed in e. coli cells by fusion with cro-beta-galactosidase proteins. | 1994 | 8091748 |
| epidemiology of genital herpes infections. | much has been learned in the last decade about the epidemiology of genital herpes infections, including new information about seroprevalence and the risk of transmission of genital herpes to sex partners and at delivery. unfortunately, the type-specific serologic assays now routinely used in these studies are not widely available, and commercially available assays that claim to be type-specific are not. thus, most clinicians still do not have access to reliable type-specific assays. in cross-sec ... | 1993 | 8106731 |
| new compounds related to podophyllotoxin and congeners: synthesis, structure elucidation and biological testing. | 4-azido, 4-amino, 4-amido and 4-alkoxy compounds related to the lignans podophyllotoxin and 4'-demethylepipodophyllotoxin have been synthesized, and their structures elucidated. the ritter reaction was shown to be useful in the preparation of the 4-amido compounds with the required stereochemistry. a preparative method for 4-chloro-4-deoxypicrophyllotoxin, for which all earlier synthetic attempts resulted in the two dehydrated compounds, alpha- and beta-apopicropodophyllotoxin, was developed. su ... | 1993 | 8110531 |
| hsv-2 dna persistence in astrocytes of the trigeminal root entry zone: double labeling by in situ pcr and immunohistochemistry. | a previous study using an in situ polymerase chain reaction (pcr) amplification method showed persistent herpes simplex virus type 2 (hsv-2) dna sequences in brains of experimentally infected mice, particularly in cells of the pons near the trigeminal root entry zone. the present study was undertaken to identify the cns cell type(s) that persistently harbor hsv dna and to define the associated pathology. tissue sections including the trigeminal root were immunoreacted to detect cellular antigens ... | 1994 | 8120536 |
| disseminated herpes simplex virus infection during pregnancy. a case report. | hepatitis due to herpes simplex virus (hsv) developed in a pregnant women at 38 weeks' gestation. she delivered a live-born infant who had serologically documented hsv 2 infection but did well with acyclovir therapy. the mother, however, died five days postpartum from fulminant hepatic failure despite antiviral treatment, and hsv was demonstrated in the liver. twenty-three reported cases clearly establish pregnancy as a condition that can predispose to disseminated hsv infection. the majority of ... | 1993 | 8120855 |
| neonatal herpes policy in the netherlands. five years after a consensus conference. | in order to establish whether the recommendations of an in 1987 organized consensus conference about neonatal herpes policy in the netherlands had been followed, an inquiry was held in january 1992 among gynecologists, pediatricians and microbiologists. compared with the results of an inquiry that was held five years before, it was found that the incidence of neonatal herpes had not increased in the last ten years (approximately five cases annually or one per 35,000 neonates) despite the lower f ... | 1993 | 8126633 |
| antiviral activity of nerve growth factor in vitro. | the antiviral activity of 'nerve growth factor' (ngf) on non-oncogenic dna, on rna viruses and on moloney sarcoma retrovirus was evaluated in vitro. ngf was active against herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2) and moloney sarcoma virus (msv) at non toxic concentrations. the effects of different treatment regimens on hsv-1 infections indicate that the inhibitory action of ngf occurs at the early stages of viral replication. no activity was noted against coxsacki ... | 1993 | 8131710 |
| human t cell lymphotropic virus type i infection among female sex workers in peru. | four hundred female sex workers attending a sexually transmitted disease clinic in lima, peru, were interviewed for demographic information and medical, contraceptive, and sexual practice histories. cervical cultures were done for neisseria gonorrhoeae and chlamydia trachomatis, and serum was tested for antibodies to human immunodeficiency virus, human t cell lymphotropic virus type i (htlv-i), treponema pallidum, c. trachomatis, herpes simplex virus type 2 (hsv-2), and haemophilus ducreyi. the ... | 1994 | 8133088 |
| immunotherapy of acute and recurrent herpes simplex virus type 2 infection with an adjuvant-free form of recombinant glycoprotein d-interleukin-2 fusion protein. | previous studies demonstrated that the adjuvant-free form of a fusion protein consisting of a truncated herpes simplex virus type 1 (hsv-1) glycoprotein d and human interleukin-2 (tgd-il-2) elicited superior protective immunity in mice. in this study, the immunotherapeutic efficacy of tgd-il-2 against vaginal hsv-2 infection was investigated using a guinea pig model. footpad injections of tgd-il-2 (12.5 micrograms/dose) after the onset of primary lesions strongly suppressed recurrence in the chr ... | 1994 | 8133093 |
| a rapid screen test for in vitro susceptibility of clinical herpes simplex virus isolates. | a rapid screening test for antiviral susceptibility to acyclovir and foscarnet was evaluated in 320 herpes simplex virus (hsv) isolates from 197 patients. of the isolates tested (16% hsv-1, 84% hsv-2), 60% were resistant in vitro to acyclovir and 5% were resistant to foscarnet using the plaque reduction assay. the rapid screening test, requiring only 3 days to yield a preliminary susceptibility result, was highly associated with the results of the plaque reduction assay for acyclovir and foscarn ... | 1994 | 8133105 |
| diagnosis of genital herpes by polymerase chain reaction amplification. | a new polymerase chain reaction (pcr) method employing type-specific primers and probes was applied to 114 clinical specimens obtained from 58 female patients with genital lesions or who had a history of genital herpes. ten and 15 specimens, respectively, were positive for herpes simplex virus (hsv)-1 and hsv-2 by cell culture. all of 10 culture-confirmed hsv-1 cases and 11 of 15 (73%) culture-confirmed hsv-2 cases were identified by pcr. although there were several cases with discrepancy betwee ... | 1993 | 8133806 |
| [investigation on the incidence of genital herpes in different professional groups in qingdao]. | genital herpes is one of 8 legally reportable sexually transmitted diseases (std) in china. using a hsv antigen elisa kit we detected and typed hsv antigen in 1,148 clinical specimens collected from the genital organs (penis, cervix, vagina and vulva) of 446 men and 702 women in qindao and divided into 11 different professional and 2 special groups (patients with cervical cancer and pregnant women). the highest positive rate of hsv antigen was found among long-distance transport drivers (48.0%). ... | 1993 | 8137406 |
| [molecular epidemiologic investigation on hsv infection among married women at childbearing age]. | by pcr and elisa, an investigation on hsv infection of cervix was carried out among 120 married women. the results indicated that the total infection rate was 23.3% (28/120). in hsv infection of cervix, hsv-2 constituted 89.3%. the positive rate of hsv in the cervicitis group and normal control group was 28.9% (26/90) and 6.7% (2/30), respectively, showing a marked difference: the positive rate in the group from 21 to 30 years old was 16.7% (7/43), from 31 to 40 was 34.6% (9/26); the positive ra ... | 1993 | 8143322 |
| tests for detecting herpes simplex virus and varicella-zoster virus infections. | several laboratory diagnostic methods are available for the diagnosis, differentiation, and subtyping of hsv and vzv infections. in the office or at the bedside of a hospitalized patient, a positive tzanck smear preparation is an inexpensive, rapid, and morphologic technique for confirming a suspected diagnosis of a herpesvirus infection. an expedient, slightly more expensive, reliable technique for establishing a hsv infection, yet not able to differentiate the subtype of that infection, is a r ... | 1994 | 8143385 |
| [ileocolitis caused by herpes simplex virus type 2]. | the authors report the case of a 51-year-old woman who was hospitalized for severe acute colitis. sub-total colectomy was performed, due to clinical deterioration and resistance to treatment. the examination of the surgery specimen revealed a herpes-virus type 2 as the responsible pathogen agent. complementary aciclovir treatment cured the patient. | 1993 | 8143954 |
| hla class i molecules are not transported to the cell surface in cells infected with herpes simplex virus types 1 and 2. | to assess the effect of herpes simplex virus (hsv) on assembly and transport of class i mhc molecules, we compared class i mhc immunoprecipitated from metabolically labeled infected and uninfected human dermal fibroblasts. the immunoprecipitates were analyzed by isoelectric focusing, allowing identification of individual class i alleles and assessment of transport through the golgi apparatus by the sialation of carbohydrate residues. in cells infected with wild-type hsv, class i synthesis was re ... | 1994 | 8144880 |
| a mouse model for studies of mucosal immunity to vaginal infection by herpes simplex virus type 2. | the role of mucosal immunity in defense of the female genital tract against pathogens such as herpes simplex virus-2 (hsv-2) is poorly understood. here we explored the use of a new mouse model to determine whether local immune events in the vagina of immune animals may protect them against genital herpes. | 1994 | 8145530 |
| [ribonucleoside and 2'-deoxyribonucleoside 5'-phosphonates: synthesis and antiviral activity]. | groups of 5'-phosphonates of natural 2'-deoxynucleosides and ribonucleosides were synthesized by condensation of 3'-acetylated 2'-deoxynucleosides or 2',3'-substituted (2',3'-o-isopropylidene, 2',3'-o-methoxymethylene, or 2',3'-o-ethoxymethylene) ribonucleosides. as condensing agents, either n,n'-dicyclohexylcarbodiimide or 2,4,6-triisopropylbenzenesulphonyl chloride were used. nucleoside 5'-ethoxycarbonyl-phosphonates were converted into corresponding nucleoside 5'-aminocarbonylphosphonates by ... | 1994 | 8145753 |
| herpes simplex virus infections of women and their offspring: implications for a developed society. | herpes simplex virus infections of humans have been known since ancient times. contemporary society has witnessed a series of devastating manifestations of herpes simplex virus infections--namely, genital herpes simplex virus infection and neonatal herpes simplex virus infection. with the evolution of society, particularly advances in birth control and increasing promiscuity, the seroprevalence of herpes simplex virus type 2 infections has increased worldwide, however, more so in developed socie ... | 1994 | 8146137 |
| efficacy of a-73209, a potent orally active agent against vzv and hsv infections. | a-73209 is a novel oxetanocin derivative with potent in vitro and in vivo activity against vzv, hsv-1, and hsv-2. a-73209 was two logs more potent than acyclovir against five thymidine kinase positive (tk+) strains of vzv in vitro (mean ec50 0.01 vs. 1.22 micrograms/ml). the activity of a-73209 was one log more potent than acyclovir against tk+ hsv-1 strains in vitro (ec50 = 0.03 vs. 0.32 micrograms/ml). a-73209 yielded a mean ec50 of 2.2 micrograms/ml compared to a mean ec50 of 0.37 micrograms/ ... | 1994 | 8147583 |
| photodynamic inactivation of herpes viruses with phthalocyanine derivatives. | the antiviral photosensitization capacity of 11 different phthalocyanine (pc) derivatives was examined using herpes simplex virus-1, herpes simplex virus-2 and varicella zoster virus in the search for the most potent sensitizers for viral decontamination of blood. the kinetics of viral photoinactivation were resolved during the stages of viral adsorption and penetration into the host cells. the capacity of pc in the photodynamic inactivation of viruses was compared with that of merocyanine 540 ( ... | 1994 | 8151454 |
| identification of a dimerization domain in the c-terminal segment of the ie110 transactivator protein from herpes simplex virus. | the 775-amino-acid ie110 (or icp0) phosphoprotein of herpes simplex virus (hsv) functions as an accessory transcription factor during the lytic cycle and plays a critical role in reactivation from latent infection. by immunofluorescence analysis, ie110 localizes in a novel pattern consisting of several dozen spherical punctate granules in the nuclei of dna-transfected cells. we constructed a hybrid version of ie110 that contained an epitope-tagged domain from the n terminus of the hsv ie175 prot ... | 1994 | 8151788 |
| sexually transmitted diseases and other risk factors for cervical dysplasia among southwestern hispanic and non-hispanic white women. | to assess risk factors for high-grade cervical dysplasia among southwestern hispanic and non-hispanic white women. | 1994 | 8151876 |
| [herpesvirus pneumonia]. | 1994 | 8151919 | |
| passive immunization of the vagina protects mice against vaginal transmission of genital herpes infections. | vaginal application of human herpes simplex virus (hsv) antiserum, complement-inactivated antiserum, or igg purified from antiserum protected mice (p < .001, p < .001, and p < .01, respectively) from visible signs of genital hsv-2 infection after subsequent vaginal inoculation with hsv-2 (10 id50). vaginal application of an anti-hsv-2 monoclonal antibody (mab iii-174) also protected mice against infection. this mab, a neutralizing mouse igg2a against glycoprotein d, prevented infection as determ ... | 1994 | 8158042 |
| 8-hydroxymanzamine a, a beta-carboline alkaloid from a sponge, pachypellina sp. | the previously described manzamine a [1] and the new 8-hydroxymanzamine a [2] were isolated from an undescribed sponge, pachypellina sp. both compounds exhibit moderate antitumor and anti-hsv-ii activity. | 1994 | 8158160 |
| vaccinating guinea pigs with recombinant glycoprotein d of herpes simplex virus in an efficacious adjuvant formulation elicits protection against vaginal infection. | guinea pigs were immunized with glycoprotein gd-2t in saf-m or saline, then challenged with herpes simplex virus, type 2 (hsv-2). animals given gd-2t in saf-m had higher anti-gd-2t antibodies, fewer and less severe vaginal lesions, and decreased ganglionic latency compared to animals given gd-2t in saline. leucocytes from animals vaccinated with gd-2t in saf-m had greater proliferative responses to gd-2t in vitro than cells from control animals. mhc ii-restricted, gd-2t-specific cytotoxic t cell ... | 1994 | 8165851 |
| disposition of antigen-presenting liposomes in vivo: effect on presentation of herpes simplex virus antigen rgd. | antigen-presenting liposomes (apls) containing a lipophilic derivative of muramyl tripeptide (mtp-pe) have previously been shown to enhance the immunotherapeutic effects mediated by hsv recombinant protein gd (rgd) after hsv type 2 infection is established. in this study, both the in vivo disposition of rgd and the immunological activity of in vivo-delivered rgd were determined. following intravenous administration, most of the liposome-encapsulated rgd accumulated rapidly, mainly in the spleen, ... | 1994 | 8165856 |
| animal models of viral sexually transmitted diseases. | 1994 | 8166948 | |
| quantity of latency-associated transcript produced by herpes simplex virus is not predictive of the frequency of experimental recurrent genital herpes. | the role of the latency-associated transcript (lat) in control of recurrent herpes simplex virus type 2 (hsv-2) infection was investigated by examining whether lat concentration in vitro during productive infection or in ganglia during latency correlated with frequency of recurrent genital herpes. clinical hsv-2 isolates from frequent or infrequent recurrent genital disease produced comparable amounts of glycoprotein d and infected cell polypeptide 0 rna, but the isolate from frequent disease pr ... | 1994 | 8169396 |
| neonatal herpes simplex virus infection: pathogenesis and treatment in the guinea pig. | intranasal inoculation of newborn guinea pigs with herpes simplex virus (hsv) resulted in local skin-eye-mouth (sem), central nervous system (cns), and disseminated infections with high but not universal mortality. cutaneous hsv inoculation produced self-limited sem infection without evidence of cns or disseminated disease. hsv infection of the upper respiratory tract of the newborn guinea pig resulted in early spread to the cns and frequent viral dissemination. the outcome of infection was favo ... | 1994 | 8169425 |
| direct recovery of herpes simplex virus (hsv)-specific t lymphocyte clones from recurrent genital hsv-2 lesions. | t lymphocytes were recovered from recurrent vesicular and ulcerative herpes simplex virus type 2 (hsv-2) lesions from 4 patients and cloned without exogenous secondary antigenic stimulation. resultant cultures were screened for antigen-specific proliferative and cytotoxic responses. of the 39 hsv-specific clones recovered, all were cd3-positive; 38 were cd4-positive and 1 was cd8-positive. of the t cell clones recovered directly from lesions, 6%-10% had hsv-specific proliferative responses, in c ... | 1994 | 8169426 |
| prevalence and epidemiologic correlates of human t cell lymphotropic virus infection among intravenous drug users. | intravenous drug users (ivdus) in seattle (n = 213) were studied to identify the prevalence and predominant types of and risk factors for human t cell lymphotropic virus (htlv) infection. detailed questionnaires, serologic screening, and polymerase chain reaction analysis (for a subset) were used. evidence of htlv infection was found in 16.5%, of which 89% were htlv-ii. htlv infection was significantly associated with nonwhite race, older age, more years of intravenous drug use, prior use of her ... | 1994 | 8169427 |
| [biological properties of hemagglutinin of herpes simplex virus type 1]. | herpes simplex virus type 1 (hsv-1) has been recently known to agglutinate mouse erythrocytes. we performed the hemagglutination (ha) test under various conditions to investigate the nature of hemagglutinin of hsv-1. ha of mouse erythrocytes was observed with glycoprotein c (gc)-positive hsv-1 strains but not with gc-negative hsv-1 mutants and hsv-2 strains. a recombinant hsv-1 constructed by transfecting a gc-negative strain with a gc-1 gene regained full ha activity. the erythrocytes from seve ... | 1994 | 8175105 |
| in situ polymerase chain reaction: localization of hsv-2 dna sequences in infections of the nervous system. | to detect and localize a herpes simplex virus type 2 (hsv-2) thymidine kinase gene sequence in paraffin sections of brains and trigeminal ganglia of infected mice, an in situ polymerase chain reaction (ispcr) protocol was developed. using a single pair of primers, a 110 base pair dna target sequence, and incorporation of a digoxigenin-labelled nucleotide during amplification, this procedure permitted rapid, specific, reproducible detection of infected cells. during acute brain infection, cells l ... | 1994 | 8175948 |
| the transmembrane domain of the large subunit of hsv-2 ribonucleotide reductase (icp10) is required for protein kinase activity and transformation-related signaling pathways that result in ras activation. | the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) is a chimera consisting of a ser/thr protein kinase (pk) with features of a transmembrane (tm) helical segment localized at the amino terminus, and the rr1 domain localized at the carboxy terminus. to elucidate the role of the tm segment in icp10-mediated transformation we established cell lines that constitutively express icp10 (jhla1) or its tm deleted mutant p139tm (jhl15). icp10 was associated with purified jhl ... | 1994 | 8178446 |
| the concept of immune-based therapies in chronic viral infections. | treatment of recurrent herpes simplex virus (hsv) infection with a vaccine was attempted as early as 1938. the concept of immune therapy is based on the premise that vaccine administration may augment host immunity, resulting in better control of the infection. the guinea pig model is useful in the study of recurrent hsv infection for several reasons. this animal model mimics human infection, with self-limited primary vulvovaginitis developing after inoculation with hsv. despite a full range of ... | 1994 | 8182508 |
| a rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe: a comparison with immunohistochemical methods for hsv detection. | we examined 28 paraffin-embedded tissue specimens with histologic evidence of herpes virus infection by in situ hybridization (ish) utilizing manual capillary action technology (microprobe staining system) and a 21 base synthetic multibiotinylated oligonucleotide probe from the hsv glycoprotein c region. the results were compared to a rapid simple immunohistochemical (ihc) protocol for detection of hsv proteins. hsv was detected by ish and ihc in all but one specimen which was shown to be positi ... | 1994 | 8189321 |
| cloning and expression of an equine herpesvirus 1 origin-binding protein. | equine herpesvirus 1 (ehv-1) is an important pathogen of horses and is closely related to several important human pathogens, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) and varicella-zoster virus. the ehv-1 genome contains open reading frames similar in sequence to the hsv-1 replication genes. pcr was used to clone ehv-1 gene 53, which is similar in sequence to the hsv-1 ul9 gene. the gene 53 product has regions of striking similarity to the hsv-1 ul9 and vzv gene 51 products. in vitro ... | 1994 | 8189505 |
| neuron-specific restriction of a herpes simplex virus recombinant maps to the ul5 gene. | we have previously shown that, when compared with either parent, a herpes simplex virus type 1/herpes simplex virus type 2 intertypic recombinant (r13-1) is attenuated by 10,000-fold with respect to neurovirulence in mice. despite this, after intracranial inoculation, r13-1 replicated to titers of 10(5) pfu per brain. we present evidence that the restriction is specific for replication in neurons and have taken a three-step approach in determining the basis of the attenuation by (i) characterizi ... | 1994 | 8189514 |
| mannan-binding protein and bovine conglutinin mediate enhancement of herpes simplex virus type 2 infection in mice. | a broad range of plant lectins have recently been shown to inhibit the infectivity of herpes simplex virus type 1 (hsv-1) in vitro. we decided to investigate the role of mammalian lectins in infection with herpes simplex virus. two lectins, conglutinin and mannan-binding protein (also called mannose-binding protein, mbp), belonging to the collectin family of lectins, were examined. four week-old balb/c mice were injected subcutaneously with 100 micrograms bovine conglutinin or 50 micrograms huma ... | 1994 | 8191219 |
| a randomized, double-blind, comparative trial comparing high- and standard-dose oral acyclovir for first-episode genital herpes infections. | orally administered acyclovir ameliorates the clinical course and decreases the duration of viral shedding in patients with first-episode genital herpes infections. we investigated in a randomized, double-blind, comparative trial whether a higher (4 g) than standard (1 g) daily dose of oral acyclovir results in greater clinical benefit and influences the time to first recurrence. a total of 139 patients with first-episode genital herpes were randomized to receive orally 4 or 1 g of acyclovir dai ... | 1994 | 8192438 |
| autopsy findings in two cases of neonatal herpes simplex virus infection: detection of virus by immunohistochemistry, in situ hybridization and the polymerase chain reaction. | we describe the pathological findings in two fatal cases of neonatal infection with herpes simplex virus. one had an encephalitis caused by herpes simplex virus type 2 (hsv-2); the other had a disseminated infection with herpes simplex virus type 1 (hsv-1). confirmation of the diagnosis was obtained by use of the polymerase chain reaction to amplify viral dna from paraffin sections of autopsy tissues. by using primers which amplify fragments of the hsv-1 thymidine kinase gene and hsv-2 glycoprot ... | 1994 | 8200626 |
| beta-carbolines from the blue-green alga dichothrix baueriana. | three new chlorine-containing beta-carbolines, bauerines a-c (1-3), have been isolted from the terrestrial blue-green alga dichothrix baueriana go-25-2, and identifying mass and nmr spectral analysis. the alkaloids show activity against herpes simplex virus type 2. | 1994 | 8201316 |
| acyclovir-resistant neonatal herpes simplex virus infection of the larynx. | a 10-day-old infant with stridor was found to have herpes simplex virus type 2 infection of the larynx. the infant's poor clinical response to both acyclovir and foscarnet prompted extensive clinical and virologic evaluations, which revealed acyclovir-resistant herpes simplex virus. | 1994 | 8201488 |
| herpes simplex virus type 2 infection of heterosexual men attending a sexual health centre. | to identify risk factors, particularly circumcision status, associated with serological evidence of herpes simplex virus type 2 (hsv-2) infection of heterosexual men. | 1994 | 8202004 |
| sero-prevalence of herpes simplex virus type-2 among cancer cervix patients. | serum samples were collected from 140 cancer cervix patients aged between 25-60 years and also from 20 age matched, married, healthy women to serve as controls. these sera were tested for hsv-2 antibodies by elisa test and hbsag by rpha test. hsv-2 antibodies were detected in 92 (65-71%) and hbsag in 25 (17.8%) cancer cervix patients. sera from control group were negative for hsv-2 antibodies and hbsag. | 1993 | 8206501 |
| underestimation of hsv-2 seroprevalence in a high-risk population by microneutralization assay. | complement independent neutralizing antibody assays (cina) have been used in seroepidemiologic studies and in diagnostic laboratories to distinguish between antibodies to herpes simplex virus types 1 (hsv-1) and 2 (hsv-2). the accuracy of cina has not been rigorously tested against protein-specific typing assays, such as western blot. | 1993 | 8211541 |
| identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted m(r) of 32 kilodaltons. | the nucleotide sequence of an infectious laryngotracheitis virus (iltv) gene which maps immediately upstream from the glycoprotein 60 (gp60) gene was determined. the gene, designated p32, encodes a predicted polypeptide of 298 amino acids with an estimated m(r) of 32,000 daltons. the predicted protein sequence has four potential n-glycosylation sites and a signal sequence at the n-terminal region. amino acid residues in the nh2-terminal region of the p32 protein exhibit similarity to glycoprotei ... | 1993 | 8212855 |
| the hsv-1 ul45 18 kda gene product is a true late protein and a component of the virion. | previously we constructed a null mutation in the hsv-1 ul45 gene, showed that the ul45 gene was not required for growth in vero cells, and confirmed that it coded for an 18 kda protein (r.j. visalli and c.r. brandt, virology 185:419-423, 1991). in this study, we have continued our characterization of the ul45 gene and the 18 kda protein. analysis of ul45 rna revealed that the gene was expressed late and was inhibited in the presence of phosphonoacetic acid (paa), indicating it is a gamma 2 class ... | 1993 | 8212858 |
| in vitro activity of a combretum micranthum extract against herpes simplex virus types 1 and 2. | the authors demonstrate in vitro antiviral activity of a methanolic extract of combretum micranthum leaves against hsv-1 and hsv-2. this activity is present only in the extract dissolved 7 days before the assay, but not in the freshly prepared extract, thus indicating the presence of inactive precursors which undergo spontaneous transformations into active compounds. the alkaline autooxidation of the methanolic extract promotes this rapid transformation. the precursors have been identified as co ... | 1993 | 8215303 |
| serum antibodies to herpes simplex virus types i and ii in depressed patients. | 1993 | 8218610 | |
| examination of cutaneous t-cell lymphoma for human herpesviruses by using the polymerase chain reaction. | the etiology of cutaneous t-cell lymphoma remains unknown, although an association with viral infection, in particular certain retroviruses and human herpesviruses, has been suggested. the purpose of this study was to examine skin biopsies of cutaneous t-cell lymphoma for the presence of epstein-barr virus, herpes simplex virus type 1 and type 2, and human herpesvirus-6 by using the polymerase chain reaction. lesional skin biopsies from 30 patients with cutaneous t-cell lymphoma were studied. co ... | 1993 | 8227605 |
| detection of herpes simplex virus in the cerebrospinal fluid of patients with encephalitis using the polymerase chain reaction. | herpes simplex encephalitis is a neurologic emergency demanding immediate institution of specific therapy in order to prevent mortality. diagnosis, however, is a complex matter with controversy existing over the appropriateness of brain biopsy. we report the demonstration of herpes simplex virus dna by means of the polymerase chain reaction (prc) in the cerebrospinal fluid of 3 patients with herpes simplex encephalitis. one of the patients suffered from brain-stem encephalitis with high intensit ... | 1993 | 8229072 |
| brief report: recurrent acyclovir-resistant genital herpes in an immunocompetent patient. | 1993 | 8232486 | |
| herpes simplex encephalitis. early diagnosis and immune activation in the acute stage and during long-term follow-up. | from a series of in all 93 patients with herpes simplex encephalitis (hse), verified by biopsy and/or the demonstration of intrathecal synthesis of antibodies to the virus, cerebrospinal fluid (csf) and serum samples were analysed and compared with samples from 80 patients with non-hse, i.e. acute encephalitis of non-hsv origin (approximately 50% with other known aetiology, 50% of unknown origin) treated on the suspicion of hse but in whom no signs of intrathecal hsv antibody synthesis were foun ... | 1993 | 8235453 |
| a solid-phase assay for the binding of peptidic subunit association inhibitors to the herpes simplex virus ribonucleotide reductase large subunit. | the herpes simplex virus (hsv) ribonucleotide reductase is comprised of two nonidentical homodimeric subunits whose association is essential for enzymatic activity. in order to evaluate the affinity of a series of peptidic inhibitors with the enzyme's large subunit (r1), we have developed a sensitive solid-phase binding assay. the assay entails the use of a nonneutralizing antibody directed against the r1 subunit of the enzyme to immobilize either the native holoenzyme from hsv-1-infected cells ... | 1993 | 8238915 |
| the epidemiology of herpes simplex types 1 and 2 infection of the genital tract in edinburgh 1978-1991. | the changing epidemiology of genital herpes in edinburgh is described in relation to herpes simplex virus (hsv) type 1 and herpes simplex virus type 2 infection over a period of 14 years. | 1993 | 8244358 |
| mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha/beta. | herpes simplex virus (hsv) ocular virulence has been associated with strain sensitivity to mouse interferon (ifn)-alpha/beta. to identify the region of the virus genome associated with heightened resistance to this cytokine, intertypic recombinants were constructed using the intact genome of avirulent, ifn-sensitive hsv type 1 (strain 35) and xbai-digested dna from virulent, ifn-resistant hsv type 2 (strain 186). an intertypic recombinant, designated hsv-r4, was isolated which grew to titres 10- ... | 1993 | 8245849 |
| genital herpes: natural history and treatment of acute episodes. | genital herpes is a common, distressing infection whose incidence has been underestimated. recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (hsv) type 2 infections in post-adolescent populations in developed countries; many of these infections have been asymptomatic. in some geographical locations, hsv-1 infections are a common cause of first episodes. they may occur in stable, monogamous relationships and are less lik ... | 1993 | 8245890 |
| herpes simplex virus infections during the decade since the licensure of acyclovir. | the development and use of acyclovir accelerated in the area of the natural history, epidemiology, and biology of herpes simplex viruses. the development of serologic assays that differentiate hsv-1 from hsv-2 demonstrated the worldwide distribution of genital herpes, the role genital ulcers play in facilitating hiv transmission, and the high frequency in which pregnant women acquire hsv infection. the high reactivation rate of hsv-2 infections, the often silent nature of genital herpes, and its ... | 1993 | 8245896 |
| isosteres of the dna polymerase inhibitor aphidicolin as potential antiviral agents against human herpes viruses. | a variety of isosteres of the dna polymerase inhibitor aphidicolin were synthesized as potential antiherpes agents. modeling studies indicated that the bicyclooctane c, d rings of aphidicolin could be replaced by an aromatic moiety while maintaining the spatial arrangement of the hydroxyl group equivalent to the essential c18 hydroxyl group of aphidicolin. of the racemic isosteres synthesized only 13, the compound with the greatest structural similarity to aphidicolin, showed any significant ant ... | 1993 | 8246219 |
| purification and biochemical characterization of the protein kinase encoded by the us3 gene of herpes simplex virus type 2. | post-ribosomal cytoplasmic fractions from vero cells mock-infected or infected with wild-type herpes simplex virus type 2 (hsv-2) or a us3 gene-disrupted mutant of hsv-2 were fractionated with deae-cellulose chromatography, and the peak fraction of the protein kinase which was detectable only in the extract of wild-type virus-infected cells was subjected to successive chromatography. the enzyme was purified more than 1000-fold from the post-ribosomal supernatant, and the final preparation contai ... | 1993 | 8249291 |
| synergistic anti-hsv effect of tumor necrosis factor alpha and interferon gamma in human corneal fibroblasts is associated with interferon beta induction. | hsv-1 (17) replicated to high titer in human corneal fibroblasts (> 10(8) pfu/10(5) cells) following infection at one pfu per 100 cells. pretreatment of the cells for 24 h with 50 u/ml recombinant human tumor necrosis factor alpha (tnf-alpha) or 5 iu/ml of human interferon gamma (ifn-gamma) resulted in only modest reduction (2- to 19-fold) in virus yield. however, when the two cytokines were combined the antiviral effect was dramatically increased. there was > 1000-fold reduction in virus titer ... | 1993 | 8250541 |
| [three cases of herpes simplex virus type 2 myelitis--detection of hsv2 dna in cerebrospinal fluid]. | polymerase chain reaction (pcr) technique has been successfully used to detect herpes simplex virus (hsv) from patients with hsv encephalitis. by pcr assay capable of differentiating hsv1 and 2, we detected hsv 2 dna in cerebrospinal fluid (csf) from patients with hsv myelitis and discussed the clinical findings. three cases of hsv myelitis (a 49-year-old female, two 38- and 44-year-old males) were studied. all cases were characterized by transverse myelopathy of the thoracic cord, and two patie ... | 1993 | 8252822 |
| [herpetic vestibular neuronitis: a hypothesis]. | abundant experimental research has shown the potentially damaging effects that the herpes simplex virus (hsv) may have on peripheral or central nervous pathways. fifty subjects (ages 20-45) with labial herpes virus (hsv-1) and 15 (ages 25-35) with genital herpes virus (hsv-2) were studied through recording spontaneous and optokinetic nystagmus (okn) and using the eye-tracking-test (ett) by means of electronystagmography (eng). recording was carried out during the first two days after vesicular e ... | 1993 | 8256608 |
| the pathogenicity of a us3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice. | we have investigated the pathogenicity of a us3 protein kinase-deficient mutant (l1 br1) of herpes simplex virus type 2 (hsv-2) for 4-week-old icr mice to define the role of the viral protein kinase in virus-host interaction. when mice were intraperitoneally infected with 10(5)pfu of l1 br1, the virus disappeared from the peritoneal cavity by 2 days postinfection and failed to induce any significant histopathological changes in the liver and spleen although viral antigens were occasionally detec ... | 1993 | 8257288 |
| new approaches to the diagnosis of oral soft-tissue disease of viral origin. | molecular biology is changing the face of diagnostic medicine, and infectious diseases of the oral soft tissues are among the targets of these advances in biotechnology. as an illustration of these concepts, a pcr-based detection and typing system for human papillomaviruses (hpvs) will be discussed. a single "consensus" set of oligomeric nucleotide primers can be used to amplify a 571- to 594-base-pair region of the e1 open reading frame of hpv 6, 11, 16, and 18. these hpv types are commonly ass ... | 1993 | 8260010 |
| identification of cytomegalovirus in vitreous using the polymerase chain reaction. | a presumptive clinical diagnosis of cytomegalovirus (cmv) retinitis was made in two patients presenting with atypical ophthalmologic findings. the diagnosis was confirmed by finding specific cmv dna sequences in sampled vitreous fluid using a polymerase chain reaction (pcr) based assay. neither of these patients yielded cmv on culture or pcr of peripheral blood leucocytes, nor was cmv present in tears or urine of either patient. serum antibody titres were stable and did not help in establishing ... | 1993 | 8260155 |
| a population-based seroepidemiological study of cervical cancer. | the epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (hpv) type 16 or 18. we performed a seroepidemiological study of hpv and cervical cancer in the counties of västerbotten and norrbotten in northern sweden, a low-risk area for cervical cancer. sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood ... | 1994 | 8261434 |
| activity of the keggin polyoxotungstate pm-19 against herpes simplex virus type 2 infection in immunosuppressed mice: role of peritoneal macrophage activation. | the in vivo antiviral activity of the keggin polyoxotungstate pm-19 [k7(pti2w10o40).6h2o] against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). when pm-19 was administered intraperitoneally to immunosuppressed mice for 3 days (once daily) starting at the time of infection, it prevented death due to hsv-2 encephalitis in a dose-dependent manner (10-25 mg/kg). the in vivo anti-hsv-2 activity of pm-19 was superior to that of acyclovir. intra ... | 1993 | 8263499 |
| [detection of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) igg and igm by elisa in cord blood and sera of mothers with pregnancy complications]. | in this study, hsv-1 igg, igm and hsv-2 igg, igm antibodies by elisa in the sera of the mothers who had different kinds of obstetrical problems like abortus, stillbirth, prematurity, postmaturity, intrauterine development retardation and in the newborns' cord sera who had congenital anomalies like anencephaly, cataract and dolichocephaly were investigated. in these mothers hsv-1 igg positivity ratio was 71/73 (97.3%). there was no significant difference in the age group distribution of hsv-1 igg ... | 1993 | 8264443 |
| acyclovir diphosphate dimyristoylglycerol: a phospholipid prodrug with activity against acyclovir-resistant herpes simplex virus. | infection with herpes simplex viruses (hsvs) resistant to treatment with acyclovir (9-[(2-hydroxyethoxy)-methyl]guanine, zovirax) is a growing clinical problem in patients with aids and other immunosuppressed states. most virus isolates resistant to acyclovir are deficient or defective in virally coded thymidine kinase (tk), which converts acyclovir to acyclovir monophosphate in virus-infected cells. to restore acyclovir efficacy, we synthesized acyclovir diphosphate dimyristoylglycerol, an anal ... | 1993 | 8265634 |
| echovirus type 19 and herpes simplex type 2 infection in human placenta tissue explants. | 1. light and electron microscopy have been used to characterize echovirus 19 and herpes simplex type 2 infection of human placenta tissue in vitro. immunofluorescence, autoradiography and virus adsorption were used to determine virus replication in this system. 2. placental tissue was permissive to echovirus 19. trophoblast cells were lysed with liberation of mature virions. however, during the 48-h period of observation, few cells were damaged and the trophoblastic structure was maintained. 3. ... | 1993 | 8268819 |
| production of herpes simplex virus fluorescein labelled typing reagents. | 29 herpes simplex virus monoclonal antibodies (mabs) were produced and shown by indirect immunofluorescence assay (iifa) to be hsv type specific or hsv cross reactive. two hsv-1 and two hsv-2 specific mabs were selected as hsv typing reagents on the basis of their specificity and the fluorescence pattern produced. when directly conjugated to fluorescein both reagents detected and correctly identified hsv isolates previously typed by restriction endonuclease analysis (rea). no cross reactions wer ... | 1993 | 8270652 |
| foscarnet-resistant herpes simplex virus infection in patients with aids. | six human immunodeficiency virus-infected patients had clinical lesions of herpes simplex virus (hsv) type 2 that showed in vitro resistance to foscarnet. in each patient, lesions were unresponsive to foscarnet therapy or developed during daily suppressive foscarnet. five patients had a history of intermittent or chronic foscarnet use for the management of acyclovir-resistant hsv infection, and 1 was receiving daily foscarnet for suppression of cytomegalovirus retinitis. seven of 10 foscarnet-re ... | 1994 | 8277181 |
| recombinant human fab to glycoprotein d neutralizes infectivity and prevents cell-to-cell transmission of herpes simplex viruses 1 and 2 in vitro. | herpes simplex viruses 1 and 2 (hsv-1 and -2) are associated with a number of conditions of varying severity, which are only partially responsive to current therapies. human antibodies to the viruses offer a potential alternative. we describe here the generation of panels of human monoclonal fab fragments to hsv-1 and -2 by panning a phage display combinatorial antibody library against whole lysates from the two viruses. each lysate selected a largely distinct set of fabs, although all of the fa ... | 1994 | 8278393 |
| herpes simplex virus type 2 infection in pregnancy: asymptomatic viral excretion at delivery and seroepidemiologic survey of two socioeconomically distinct populations in são paulo, brazil. | the objective of the present study was to estimate the prevalence of herpes simplex virus type 2 (hsv 2) antibodies in child bearing women of 2 brazilian populations with different socioeconomic status and to determine the risk of neonatal hsv exposure by means of maternal cultures at the onset of labor. the study was conducted at 2 hospitals: a, serving very low income patients and b, serving middle socioeconomic class. 173 participants from group a and 127 from b answered a questionnaire which ... | 1993 | 8278760 |
| quantitative analysis of herpes simplex virus dna in cerebrospinal fluid of children with herpes simplex encephalitis. | herpes simplex virus (hsv) dna in the cerebrospinal fluid (csf) of children with herpes simplex encephalitis (hse) was quantified and typed using the polymerase chain reaction (pcr) assay. during the acute phase, hsv-dna was detected in the csf of 13 patients with hse, including 5 neonates. a restriction profile of the pcr products cleaved with the restriction enzymes xhoi and bglii showed that 2 neonatal samples were hsv-2, and the remainder were hsv-1. the amount of hsv-dna in the initial csf ... | 1993 | 8283180 |
| herpes simplex type 2 in a patient with mollaret's meningitis: demonstration by polymerase chain reaction. | we describe a patient with recurrent self-limited aseptic meningitis meeting the criteria for mollaret's syndrome, in whom extensive serological investigation and cerebrospinal fluid analysis failed to reveal a specific cause. using polymerase chain reaction techniques to amplify herpes simplex viral dna, we were able to identify the presence of herpes simplex virus type 2 in the cerebrospinal fluid. the duration of clinical illness may have been shortened by treatment with intravenous acyclovir ... | 1994 | 8285581 |
| herpes simplex virus type 2 dna in cerebrospinal fluid of a woman with recurrent meningitis. | 1993 | 8286655 | |
| latent infection with the ms strain of herpes simplex virus type 2 in the mouse following intracerebral inoculation. | intracerebral inoculation of the ms strain of herpes simplex virus type 2 (hsv-2) into mice causes an acute encephalitis associated with multifocal demyelination and necrotizing retinitis. we have studied the distribution of latent virus in mice that had recovered from the acute encephalitis. four weeks or longer after inoculation, hsv-2 could be recovered from the trigeminal ganglia of all mice examined by co-culture of explants in roller tubes. the virus could not be recovered from explants of ... | 1993 | 8289071 |
| s-glucosylated hydantoins as new antiviral agents. | s-glycosylation took place on reaction of 5-alkylidene- and 5-arylidene-3-aryl-2-thiohydantoins with glycosyl halides under alkaline conditions. bisglucosylation also took place when n-3 unsubstituted hydantoins were reacted. the bisglucosylated hydantoins produced n-3 glucosylated hydantoins on treatment with ammonia in methanol. in antiviral studies the most active compounds against both hsv-1 and hsv-2 were 5-(2-thienylmethylene)-3-phenyl-2-(2,3,4,6- tetra-o-acetyl-beta-d-glucopyranosyl)-2-th ... | 1994 | 8289203 |
| [acyclovir-resistant herpes simplex viruses in hiv-infected patients]. | 1993 | 8290931 |