Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| efficacy of oral administration of live herpes simplex virus type 1 as a vaccine. | mice given herpes simplex virus type 1 (hsv-1) (miyama +gc strain) intragastrically via a stainless-steel cannula were rendered immune to subsequent lethal intraperitoneal (i.p.) challenge with hsv-1. the orally administered hsv-1 was completely inactivated in the stomach within a few minutes of inoculation. however, systemic immunity was established 14 days after oral inoculation with the virus and retained for up to 6 months. the mechanisms of establishing systemic immunity were investigated b ... | 1992 | 1312638 |
| [the isolation and characteristics of monoclonal antibodies to different proteins of the herpes simplex virus types 1 and 2]. | the authors prepared 156 mouse hybridomas producing monoclonal (mca) antibodies to type- and group-specific antigenic determinants of hsv-1 and hsv-2. seven of them were studied at length by western blot and radioimmunoprecipitation methods. the cell lines 1h97 and 2h141 were shown to produce immunoglobulins of g2 beta and m class, respectively, and were directed against group-specific antigenic determinants of the major nucleocapsid protein p150. the cell lines 1h38 and 1h110 produced immunoglo ... | 1992 | 1281945 |
| [the inhibitory effects of catechin derivatives on the activities of human immunodeficiency virus reverse transcriptase and dna polymerases]. | catechin derivatives including (-)-epicatechin gallate (ecg), (-)-epigallocatechin gallate (egcg), (-)-epigallocatechin (egc) and green tea extract (gte) were found to inhibit the activities of cloned human immunodeficiency virus type 1 reverse transcriptase (hiv-1 rt), duck hepatitis b virus replication complexes reverse transcriptase (dhbv rcs rt), herpes simplex virus 1 dna polymerase (hsv-1 dnap) and cow thymus dna polymerase alpha (ct dnap alpha). egcg and ecg were shown to be very potent i ... | 1992 | 1284389 |
| herpes simplex virus type 2 and pseudorabies virus associated growth factors and their role in the latency in vitro. | a putative herpes simplex virus type 2 (hsv-2) growth factor (hsgf-2) was detected in a crude extract from virus infected mouse embryo cells. this factor, similar to previously described pseudorabies virus (prv) associated growth factor (prgf) was shown to have ability to morphologically transform non-transformed cells and to repress the transformed phenotype of transformed cells. both activities could be neutralized with two, out of seven monoclonal antibodies directed against glycoprotein b of ... | 1992 | 1284865 |
| herpes simplex virus life cycle and the design of viral vectors. | herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2) are doublestranded dna viruses with a genome size of 152 kbp. the genome consists of two unique regions, u(l) (long) and u(s) (short), flanked by repeated sequences (fig. 1; for review, see ref. 1). the two viruses are closely related, and both infect humans, producing mucocutaneous sores that are predominantly facial in the case of hsv-1 and genital in the case of hsv-2. | 1992 | 21390711 |
| immunogenicity in mice of tandem repeats of an epitope from herpes simplex gd protein when expressed by recombinant adenovirus vectors. | the antigenic and immunogenic potential was examined of human adenovirus type 5 (ad) recombinants carrying and expressing from one to four tandem repeats of a linear neutralizing epitope from the gd protein of herpes simplex virus type 1 (hsv-1) as a fusion with the beta-galactosidase protein. the fusion proteins produced by these ad vectors in infected cell culture reacted with a herpes simplex virus (hsv) epitope-specific monoclonal antibody to a degree dependent on the number of epitope repea ... | 1993 | 7504857 |
| bleomycin differentially inhibits picornavirus, herpesvirus and poxvirus replication in a human carcinoma cell line. | ms-757, a cervix carcinoma cell line, was exposed to bleomycin at concentrations up to 200 microm for periods of 1 to 24 hours. bleomycin treatment caused the level of dna synthesis in uninfected cells to drop to 13% of the level achieved in the controls. protein synthesis fell to 50%, but rna synthesis was not affected. exposure of uninfected cells to 200 microm bleomycin for 24 h did not induce significant cell death measured as permeability to trypan blue). a tetrazolium dye-reduction assay, ... | 1993 | 7514894 |
| detection of immunoglobulin m antibodies to glycoprotein g-2 by western blot (immunoblot) for diagnosis of initial herpes simplex virus type 2 genital infections. | western blots (immunoblots) for the detection of immunoglobulin m (igm) antibodies specific for herpes simplex virus type 1 (hsv-1) and hsv-2 in patients' sera were developed. the locations of the type-specific glycoprotein g (gpg-2) of hsv-2 (92- and 140-kda forms) and glycoprotein c of hsv-1 (gpc-1), which carries mostly type-specific antigenic epitopes, were checked with specific monoclonal antibodies. western blot assays for igm antibody to gpc-1 or gpg-2 were performed after depletion of ig ... | 1993 | 7508453 |
| [recurrent cutaneous herpes in the newborn and acyclovir]. | the authors report two cases of cutaneous recurrent herpes occurring after a neonatal herpes simplex virus type 2 (hsv2) infection and comment on the role of acute or suppressive therapy by aciclovir (acv). the two infants were not treated by acv after the neonatal period. none of the recurrent cutaneous herpes episodes was followed by viral widespread. one case reported by bergström et al on a relapse of hsv2 encephalitis occurring after a cutaneous herpes in a child argues for the use of acv i ... | 1993 | 7777392 |
| ms strain of type 2 herpes simplex virus produces necrotizing retinitis in mice. | intracerebral inoculation of mice with the ms strain of type 2 herpes simplex virus (hsv-2) causes a brief encephalitis associated with multifocal central nervous system demyelination. many of the mice develop unilateral or bilateral impairment of the pupillary light reflex. we have examined the development of ocular disease in inbred nih mice inoculated intracerebrally with a low dose (10 pfu) of hsv-2 (ms). the resulting acute encephalitis was fatal in 30-50% of the mice. by 1 month after inoc ... | 1993 | 7683330 |
| biological response modifier-mediated resistance to herpesvirus infections requires induction of alpha/beta interferon. | the role of interferon alpha/beta (ifn) induction by the immunomodulators piclc and cl246,738 was investigated in cd-1 mice infected with murine cytomegalovirus (mcmv) or herpes simplex virus type 2 (hsv-2). mice were treated with either normal sheep serum or anti-alpha/beta ifn antiserum, inoculated with the immunomodulators, and infected with virus. because anti-ifn treatment also decreased natural resistance to hsv-2 and mcmv, two viral challenge doses were used to ensure that the mice with c ... | 1993 | 7687839 |
| antibodies to epitopes of herpes simplex virus type 1 glycoprotein b (gb) in human sera: analysis of functional gb epitopes defined by inhibition of murine monoclonal antibodies. | the epitopes on herpes simplex virus (hsv) glycoprotein b (gb) recognized by sera of 23 patients with well-characterized hsv infection were studied. twelve epitope-specific monoclonal antibodies with neutralization (nt) or antibody-dependent cellular cytotoxicity (adcc) activities were used in competitive elisa binding inhibition studies. the sera were additionally analyzed for homologous viral type nt, adcc activity, and gb-reactive antibody by elisa. seroconversion was observed in each assay d ... | 1993 | 7690824 |
| the inhibitory effect of spermicidal agents on replication of hsv-2 and hiv-1 in-vitro. | five spermicides including nonoxynol-9 were assessed under in-vitro conditions, for their inhibitory activity against two viruses capable of spread by sexual intercourse, herpes simplex virus type-2 (hsv-2) and the human immunodeficiency virus type-1. a further eight commercially-available spermicidal preparations containing varying concentrations of either nonoxynol-9 or nonoxynol-11 were also assessed for activity against hsv-2. all spermicides and spermicidal preparations tested showed inhibi ... | 1993 | 7693643 |
| identification of renal sympathetic preganglionic neurons in hamsters using transsynaptic transport of herpes simplex type 1 virus. | herpes viruses have been used as retrograde transsynaptic tracers to identify pathways from the cns to specific target tissues. we used herpes simplex virus to identify central nervous system neurons responsible for control of the kidney. herpes simplex type 1 or herpes simplex type 2 was injected into rat kidneys and herpes simplex type 1 was microinjected into hamster and guinea pig kidneys. after three to seven days, ganglia, spinal cords and brains were examined using immunohistochemistry to ... | 1993 | 7694186 |
| analysis of the epidemiology and pathogenesis of herpes simplex virus (hsv) infections in pregnant women and infants using the hsv-2 glycoprotein g antibody assay. | 1993 | 8012738 | |
| antiviral activity of nerve growth factor in vitro. | the antiviral activity of 'nerve growth factor' (ngf) on non-oncogenic dna, on rna viruses and on moloney sarcoma retrovirus was evaluated in vitro. ngf was active against herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2) and moloney sarcoma virus (msv) at non toxic concentrations. the effects of different treatment regimens on hsv-1 infections indicate that the inhibitory action of ngf occurs at the early stages of viral replication. no activity was noted against coxsacki ... | 1993 | 8131710 |
| epidemiology of genital herpes infections. | much has been learned in the last decade about the epidemiology of genital herpes infections, including new information about seroprevalence and the risk of transmission of genital herpes to sex partners and at delivery. unfortunately, the type-specific serologic assays now routinely used in these studies are not widely available, and commercially available assays that claim to be type-specific are not. thus, most clinicians still do not have access to reliable type-specific assays. in cross-sec ... | 1993 | 8106731 |
| [antiviral activity of mangiferin against herpes simplex virus type 2 in vitro]. | the effect of mangiferin, a tetrahydroxy pyrrolidone saponin extracted from the leaves of mango (mangifera indica), against herpes simplex virus type 2 (hsv-2) in vitro was studied. the 50% effective concentration (ec50) of it against hsv-2 plaque formation in hela cells was 111.7 micrograms.ml-1, and the concentrations of 33 and 80 micrograms.ml-1 reduced the virus replicative yields by 90% (ec90) and 99% (ec99), respectively. the therapeutic index (ic50/ec50) was 8.1. mangiferin did not direct ... | 1993 | 8010041 |
| [the primary immune response and virus-specific and -nonspecific immune complexes in neonatal cytomegalovirus and herpetic infection]. | 1993 | 8079522 | |
| [molecular epidemiologic investigation on hsv infection among married women at childbearing age]. | by pcr and elisa, an investigation on hsv infection of cervix was carried out among 120 married women. the results indicated that the total infection rate was 23.3% (28/120). in hsv infection of cervix, hsv-2 constituted 89.3%. the positive rate of hsv in the cervicitis group and normal control group was 28.9% (26/90) and 6.7% (2/30), respectively, showing a marked difference: the positive rate in the group from 21 to 30 years old was 16.7% (7/43), from 31 to 40 was 34.6% (9/26); the positive ra ... | 1993 | 8143322 |
| diagnosis of genital herpes by polymerase chain reaction amplification. | a new polymerase chain reaction (pcr) method employing type-specific primers and probes was applied to 114 clinical specimens obtained from 58 female patients with genital lesions or who had a history of genital herpes. ten and 15 specimens, respectively, were positive for herpes simplex virus (hsv)-1 and hsv-2 by cell culture. all of 10 culture-confirmed hsv-1 cases and 11 of 15 (73%) culture-confirmed hsv-2 cases were identified by pcr. although there were several cases with discrepancy betwee ... | 1993 | 8133806 |
| [investigation on the incidence of genital herpes in different professional groups in qingdao]. | genital herpes is one of 8 legally reportable sexually transmitted diseases (std) in china. using a hsv antigen elisa kit we detected and typed hsv antigen in 1,148 clinical specimens collected from the genital organs (penis, cervix, vagina and vulva) of 446 men and 702 women in qindao and divided into 11 different professional and 2 special groups (patients with cervical cancer and pregnant women). the highest positive rate of hsv antigen was found among long-distance transport drivers (48.0%). ... | 1993 | 8137406 |
| new compounds related to podophyllotoxin and congeners: synthesis, structure elucidation and biological testing. | 4-azido, 4-amino, 4-amido and 4-alkoxy compounds related to the lignans podophyllotoxin and 4'-demethylepipodophyllotoxin have been synthesized, and their structures elucidated. the ritter reaction was shown to be useful in the preparation of the 4-amido compounds with the required stereochemistry. a preparative method for 4-chloro-4-deoxypicrophyllotoxin, for which all earlier synthetic attempts resulted in the two dehydrated compounds, alpha- and beta-apopicropodophyllotoxin, was developed. su ... | 1993 | 8110531 |
| disseminated herpes simplex virus infection during pregnancy. a case report. | hepatitis due to herpes simplex virus (hsv) developed in a pregnant women at 38 weeks' gestation. she delivered a live-born infant who had serologically documented hsv 2 infection but did well with acyclovir therapy. the mother, however, died five days postpartum from fulminant hepatic failure despite antiviral treatment, and hsv was demonstrated in the liver. twenty-three reported cases clearly establish pregnancy as a condition that can predispose to disseminated hsv infection. the majority of ... | 1993 | 8120855 |
| neonatal herpes policy in the netherlands. five years after a consensus conference. | in order to establish whether the recommendations of an in 1987 organized consensus conference about neonatal herpes policy in the netherlands had been followed, an inquiry was held in january 1992 among gynecologists, pediatricians and microbiologists. compared with the results of an inquiry that was held five years before, it was found that the incidence of neonatal herpes had not increased in the last ten years (approximately five cases annually or one per 35,000 neonates) despite the lower f ... | 1993 | 8126633 |
| [ileocolitis caused by herpes simplex virus type 2]. | the authors report the case of a 51-year-old woman who was hospitalized for severe acute colitis. sub-total colectomy was performed, due to clinical deterioration and resistance to treatment. the examination of the surgery specimen revealed a herpes-virus type 2 as the responsible pathogen agent. complementary aciclovir treatment cured the patient. | 1993 | 8143954 |
| brief report: recurrent acyclovir-resistant genital herpes in an immunocompetent patient. | 1993 | 8232486 | |
| herpes simplex encephalitis. early diagnosis and immune activation in the acute stage and during long-term follow-up. | from a series of in all 93 patients with herpes simplex encephalitis (hse), verified by biopsy and/or the demonstration of intrathecal synthesis of antibodies to the virus, cerebrospinal fluid (csf) and serum samples were analysed and compared with samples from 80 patients with non-hse, i.e. acute encephalitis of non-hsv origin (approximately 50% with other known aetiology, 50% of unknown origin) treated on the suspicion of hse but in whom no signs of intrathecal hsv antibody synthesis were foun ... | 1993 | 8235453 |
| purification and biochemical characterization of the protein kinase encoded by the us3 gene of herpes simplex virus type 2. | post-ribosomal cytoplasmic fractions from vero cells mock-infected or infected with wild-type herpes simplex virus type 2 (hsv-2) or a us3 gene-disrupted mutant of hsv-2 were fractionated with deae-cellulose chromatography, and the peak fraction of the protein kinase which was detectable only in the extract of wild-type virus-infected cells was subjected to successive chromatography. the enzyme was purified more than 1000-fold from the post-ribosomal supernatant, and the final preparation contai ... | 1993 | 8249291 |
| identification of an infectious laryngotracheitis virus gene encoding an immunogenic protein with a predicted m(r) of 32 kilodaltons. | the nucleotide sequence of an infectious laryngotracheitis virus (iltv) gene which maps immediately upstream from the glycoprotein 60 (gp60) gene was determined. the gene, designated p32, encodes a predicted polypeptide of 298 amino acids with an estimated m(r) of 32,000 daltons. the predicted protein sequence has four potential n-glycosylation sites and a signal sequence at the n-terminal region. amino acid residues in the nh2-terminal region of the p32 protein exhibit similarity to glycoprotei ... | 1993 | 8212855 |
| the hsv-1 ul45 18 kda gene product is a true late protein and a component of the virion. | previously we constructed a null mutation in the hsv-1 ul45 gene, showed that the ul45 gene was not required for growth in vero cells, and confirmed that it coded for an 18 kda protein (r.j. visalli and c.r. brandt, virology 185:419-423, 1991). in this study, we have continued our characterization of the ul45 gene and the 18 kda protein. analysis of ul45 rna revealed that the gene was expressed late and was inhibited in the presence of phosphonoacetic acid (paa), indicating it is a gamma 2 class ... | 1993 | 8212858 |
| in vitro activity of a combretum micranthum extract against herpes simplex virus types 1 and 2. | the authors demonstrate in vitro antiviral activity of a methanolic extract of combretum micranthum leaves against hsv-1 and hsv-2. this activity is present only in the extract dissolved 7 days before the assay, but not in the freshly prepared extract, thus indicating the presence of inactive precursors which undergo spontaneous transformations into active compounds. the alkaline autooxidation of the methanolic extract promotes this rapid transformation. the precursors have been identified as co ... | 1993 | 8215303 |
| detection of herpes simplex virus in the cerebrospinal fluid of patients with encephalitis using the polymerase chain reaction. | herpes simplex encephalitis is a neurologic emergency demanding immediate institution of specific therapy in order to prevent mortality. diagnosis, however, is a complex matter with controversy existing over the appropriateness of brain biopsy. we report the demonstration of herpes simplex virus dna by means of the polymerase chain reaction (prc) in the cerebrospinal fluid of 3 patients with herpes simplex encephalitis. one of the patients suffered from brain-stem encephalitis with high intensit ... | 1993 | 8229072 |
| genital herpes: natural history and treatment of acute episodes. | genital herpes is a common, distressing infection whose incidence has been underestimated. recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (hsv) type 2 infections in post-adolescent populations in developed countries; many of these infections have been asymptomatic. in some geographical locations, hsv-1 infections are a common cause of first episodes. they may occur in stable, monogamous relationships and are less lik ... | 1993 | 8245890 |
| identification of cytomegalovirus in vitreous using the polymerase chain reaction. | a presumptive clinical diagnosis of cytomegalovirus (cmv) retinitis was made in two patients presenting with atypical ophthalmologic findings. the diagnosis was confirmed by finding specific cmv dna sequences in sampled vitreous fluid using a polymerase chain reaction (pcr) based assay. neither of these patients yielded cmv on culture or pcr of peripheral blood leucocytes, nor was cmv present in tears or urine of either patient. serum antibody titres were stable and did not help in establishing ... | 1993 | 8260155 |
| underestimation of hsv-2 seroprevalence in a high-risk population by microneutralization assay. | complement independent neutralizing antibody assays (cina) have been used in seroepidemiologic studies and in diagnostic laboratories to distinguish between antibodies to herpes simplex virus types 1 (hsv-1) and 2 (hsv-2). the accuracy of cina has not been rigorously tested against protein-specific typing assays, such as western blot. | 1993 | 8211541 |
| herpes simplex virus type 2 dna in cerebrospinal fluid of a woman with recurrent meningitis. | 1993 | 8286655 | |
| serum antibodies to herpes simplex virus types i and ii in depressed patients. | 1993 | 8218610 | |
| acyclovir diphosphate dimyristoylglycerol: a phospholipid prodrug with activity against acyclovir-resistant herpes simplex virus. | infection with herpes simplex viruses (hsvs) resistant to treatment with acyclovir (9-[(2-hydroxyethoxy)-methyl]guanine, zovirax) is a growing clinical problem in patients with aids and other immunosuppressed states. most virus isolates resistant to acyclovir are deficient or defective in virally coded thymidine kinase (tk), which converts acyclovir to acyclovir monophosphate in virus-infected cells. to restore acyclovir efficacy, we synthesized acyclovir diphosphate dimyristoylglycerol, an anal ... | 1993 | 8265634 |
| [three cases of herpes simplex virus type 2 myelitis--detection of hsv2 dna in cerebrospinal fluid]. | polymerase chain reaction (pcr) technique has been successfully used to detect herpes simplex virus (hsv) from patients with hsv encephalitis. by pcr assay capable of differentiating hsv1 and 2, we detected hsv 2 dna in cerebrospinal fluid (csf) from patients with hsv myelitis and discussed the clinical findings. three cases of hsv myelitis (a 49-year-old female, two 38- and 44-year-old males) were studied. all cases were characterized by transverse myelopathy of the thoracic cord, and two patie ... | 1993 | 8252822 |
| examination of cutaneous t-cell lymphoma for human herpesviruses by using the polymerase chain reaction. | the etiology of cutaneous t-cell lymphoma remains unknown, although an association with viral infection, in particular certain retroviruses and human herpesviruses, has been suggested. the purpose of this study was to examine skin biopsies of cutaneous t-cell lymphoma for the presence of epstein-barr virus, herpes simplex virus type 1 and type 2, and human herpesvirus-6 by using the polymerase chain reaction. lesional skin biopsies from 30 patients with cutaneous t-cell lymphoma were studied. co ... | 1993 | 8227605 |
| a solid-phase assay for the binding of peptidic subunit association inhibitors to the herpes simplex virus ribonucleotide reductase large subunit. | the herpes simplex virus (hsv) ribonucleotide reductase is comprised of two nonidentical homodimeric subunits whose association is essential for enzymatic activity. in order to evaluate the affinity of a series of peptidic inhibitors with the enzyme's large subunit (r1), we have developed a sensitive solid-phase binding assay. the assay entails the use of a nonneutralizing antibody directed against the r1 subunit of the enzyme to immobilize either the native holoenzyme from hsv-1-infected cells ... | 1993 | 8238915 |
| the epidemiology of herpes simplex types 1 and 2 infection of the genital tract in edinburgh 1978-1991. | the changing epidemiology of genital herpes in edinburgh is described in relation to herpes simplex virus (hsv) type 1 and herpes simplex virus type 2 infection over a period of 14 years. | 1993 | 8244358 |
| mapping the genetic region coding for herpes simplex virus resistance to mouse interferon alpha/beta. | herpes simplex virus (hsv) ocular virulence has been associated with strain sensitivity to mouse interferon (ifn)-alpha/beta. to identify the region of the virus genome associated with heightened resistance to this cytokine, intertypic recombinants were constructed using the intact genome of avirulent, ifn-sensitive hsv type 1 (strain 35) and xbai-digested dna from virulent, ifn-resistant hsv type 2 (strain 186). an intertypic recombinant, designated hsv-r4, was isolated which grew to titres 10- ... | 1993 | 8245849 |
| the herpes simplex virus type 2 ul3 open reading frame encodes a nuclear localizing phosphoprotein. | the herpes simplex virus type 2 (hsv-2, strain 333) ul3 open reading frame (orf) codes for a protein with a predicted molecular weight of 29,681. comparisons of the ul3, strain 333 orf show essentially complete amino acid identity with hsv-2 (strain hg52) and a 75% amino acid identity with hsv type 1 (strain 17). to characterize the expression of this gene, a hydrophilic region of the hsv-2 ul3 gene was cloned into a bacterial expression vector. the resulting fusion protein was used to generate ... | 1993 | 8337818 |
| isosteres of the dna polymerase inhibitor aphidicolin as potential antiviral agents against human herpes viruses. | a variety of isosteres of the dna polymerase inhibitor aphidicolin were synthesized as potential antiherpes agents. modeling studies indicated that the bicyclooctane c, d rings of aphidicolin could be replaced by an aromatic moiety while maintaining the spatial arrangement of the hydroxyl group equivalent to the essential c18 hydroxyl group of aphidicolin. of the racemic isosteres synthesized only 13, the compound with the greatest structural similarity to aphidicolin, showed any significant ant ... | 1993 | 8246219 |
| mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing herpes simplex virus glycoprotein b. | a recombinant adenovirus (ad) expressing glycoprotein b (gb) of herpes simplex virus (hsv) type 1 (adgb8) was evaluated as a mucosal vaccine candidate. when administered intranasally (inl) to c57b1/6 mice, adgb8 induced levels of serum anti-hsv gb igg antibodies similar to those of mice immunized intraperitoneally (ip), which neutralized both hsv-1 and -2. mice immunized inl with adgb8 produced secretory iga specific for hsv gb, but mice immunized ip did not. splenic anti-hsv cytotoxic t lymphoc ... | 1993 | 8354903 |
| new approaches to the diagnosis of oral soft-tissue disease of viral origin. | molecular biology is changing the face of diagnostic medicine, and infectious diseases of the oral soft tissues are among the targets of these advances in biotechnology. as an illustration of these concepts, a pcr-based detection and typing system for human papillomaviruses (hpvs) will be discussed. a single "consensus" set of oligomeric nucleotide primers can be used to amplify a 571- to 594-base-pair region of the e1 open reading frame of hpv 6, 11, 16, and 18. these hpv types are commonly ass ... | 1993 | 8260010 |
| sero-prevalence of herpes simplex virus type-2 among cancer cervix patients. | serum samples were collected from 140 cancer cervix patients aged between 25-60 years and also from 20 age matched, married, healthy women to serve as controls. these sera were tested for hsv-2 antibodies by elisa test and hbsag by rpha test. hsv-2 antibodies were detected in 92 (65-71%) and hbsag in 25 (17.8%) cancer cervix patients. sera from control group were negative for hsv-2 antibodies and hbsag. | 1993 | 8206501 |
| activity of the keggin polyoxotungstate pm-19 against herpes simplex virus type 2 infection in immunosuppressed mice: role of peritoneal macrophage activation. | the in vivo antiviral activity of the keggin polyoxotungstate pm-19 [k7(pti2w10o40).6h2o] against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). when pm-19 was administered intraperitoneally to immunosuppressed mice for 3 days (once daily) starting at the time of infection, it prevented death due to hsv-2 encephalitis in a dose-dependent manner (10-25 mg/kg). the in vivo anti-hsv-2 activity of pm-19 was superior to that of acyclovir. intra ... | 1993 | 8263499 |
| synergistic anti-hsv effect of tumor necrosis factor alpha and interferon gamma in human corneal fibroblasts is associated with interferon beta induction. | hsv-1 (17) replicated to high titer in human corneal fibroblasts (> 10(8) pfu/10(5) cells) following infection at one pfu per 100 cells. pretreatment of the cells for 24 h with 50 u/ml recombinant human tumor necrosis factor alpha (tnf-alpha) or 5 iu/ml of human interferon gamma (ifn-gamma) resulted in only modest reduction (2- to 19-fold) in virus yield. however, when the two cytokines were combined the antiviral effect was dramatically increased. there was > 1000-fold reduction in virus titer ... | 1993 | 8250541 |
| [herpetic vestibular neuronitis: a hypothesis]. | abundant experimental research has shown the potentially damaging effects that the herpes simplex virus (hsv) may have on peripheral or central nervous pathways. fifty subjects (ages 20-45) with labial herpes virus (hsv-1) and 15 (ages 25-35) with genital herpes virus (hsv-2) were studied through recording spontaneous and optokinetic nystagmus (okn) and using the eye-tracking-test (ett) by means of electronystagmography (eng). recording was carried out during the first two days after vesicular e ... | 1993 | 8256608 |
| the pathogenicity of a us3 protein kinase-deficient mutant of herpes simplex virus type 2 in mice. | we have investigated the pathogenicity of a us3 protein kinase-deficient mutant (l1 br1) of herpes simplex virus type 2 (hsv-2) for 4-week-old icr mice to define the role of the viral protein kinase in virus-host interaction. when mice were intraperitoneally infected with 10(5)pfu of l1 br1, the virus disappeared from the peritoneal cavity by 2 days postinfection and failed to induce any significant histopathological changes in the liver and spleen although viral antigens were occasionally detec ... | 1993 | 8257288 |
| echovirus type 19 and herpes simplex type 2 infection in human placenta tissue explants. | 1. light and electron microscopy have been used to characterize echovirus 19 and herpes simplex type 2 infection of human placenta tissue in vitro. immunofluorescence, autoradiography and virus adsorption were used to determine virus replication in this system. 2. placental tissue was permissive to echovirus 19. trophoblast cells were lysed with liberation of mature virions. however, during the 48-h period of observation, few cells were damaged and the trophoblastic structure was maintained. 3. ... | 1993 | 8268819 |
| use of isotopically chiral [4'-13c]penciclovir and 13c nmr to determine the specificity and absolute configuration of penciclovir phosphate esters formed in hsv-1 and hsv-2 infected cells and by hsv-1-encoded thymidine kinase. | penciclovir is a potent antiherpesvirus agent which is highly selective due to its phosphorylation only in virus infected cells. phosphorylation of one of the hydroxymethyl groups of penciclovir (pcv) creates a chiral centre leading to the possible formation of (r)- and (s)-enantiomers. the absolute configuration and stereospecificity of the pcv-phosphates produced in cells infected with herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), as well as by hsv-1-encoded thymidine kinase, were de ... | 1993 | 8305285 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| production of herpes simplex virus fluorescein labelled typing reagents. | 29 herpes simplex virus monoclonal antibodies (mabs) were produced and shown by indirect immunofluorescence assay (iifa) to be hsv type specific or hsv cross reactive. two hsv-1 and two hsv-2 specific mabs were selected as hsv typing reagents on the basis of their specificity and the fluorescence pattern produced. when directly conjugated to fluorescein both reagents detected and correctly identified hsv isolates previously typed by restriction endonuclease analysis (rea). no cross reactions wer ... | 1993 | 8270652 |
| flow cytometric indirect immunofluorescence assay with high sensitivity and specificity for the detection of antibodies to hsv-1 and hsv-2. | cells infected with hsv-1 or hsv-2 develop viral antigens which can be detected by immunofluorescence. we developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to hsv-1 and hsv-2 in human sera. results obtained by flow cytometry for detecting antibodies against hsv-1, when compared with results obtained by elisa, showed an index of overall agreement of 100%. the correlation between the antibody titers obtained with each method was found to be highly si ... | 1993 | 8307141 |
| baculovirus-expressed herpes simplex virus type 2 glycoprotein d is immunogenic and protective against lethal hsv challenge. | herpes simplex virus type 2 glycoprotein d (gd2) was cloned and expressed in the baculovirus-spodoptera frugiperda system. milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. the resultant purified gd existed as a homogeneous 57,500 mw monomeric species demonstrating reactivity with anti-gd monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gd. immuniza ... | 1993 | 8385842 |
| herpes simplex virus infections during the decade since the licensure of acyclovir. | the development and use of acyclovir accelerated in the area of the natural history, epidemiology, and biology of herpes simplex viruses. the development of serologic assays that differentiate hsv-1 from hsv-2 demonstrated the worldwide distribution of genital herpes, the role genital ulcers play in facilitating hiv transmission, and the high frequency in which pregnant women acquire hsv infection. the high reactivation rate of hsv-2 infections, the often silent nature of genital herpes, and its ... | 1993 | 8245896 |
| herpes simplex virus type 2 infection in pregnancy: asymptomatic viral excretion at delivery and seroepidemiologic survey of two socioeconomically distinct populations in são paulo, brazil. | the objective of the present study was to estimate the prevalence of herpes simplex virus type 2 (hsv 2) antibodies in child bearing women of 2 brazilian populations with different socioeconomic status and to determine the risk of neonatal hsv exposure by means of maternal cultures at the onset of labor. the study was conducted at 2 hospitals: a, serving very low income patients and b, serving middle socioeconomic class. 173 participants from group a and 127 from b answered a questionnaire which ... | 1993 | 8278760 |
| quantitative analysis of herpes simplex virus dna in cerebrospinal fluid of children with herpes simplex encephalitis. | herpes simplex virus (hsv) dna in the cerebrospinal fluid (csf) of children with herpes simplex encephalitis (hse) was quantified and typed using the polymerase chain reaction (pcr) assay. during the acute phase, hsv-dna was detected in the csf of 13 patients with hse, including 5 neonates. a restriction profile of the pcr products cleaved with the restriction enzymes xhoi and bglii showed that 2 neonatal samples were hsv-2, and the remainder were hsv-1. the amount of hsv-dna in the initial csf ... | 1993 | 8283180 |
| [detection of herpes simplex virus 1 and 2 (hsv-1 and hsv-2) igg and igm by elisa in cord blood and sera of mothers with pregnancy complications]. | in this study, hsv-1 igg, igm and hsv-2 igg, igm antibodies by elisa in the sera of the mothers who had different kinds of obstetrical problems like abortus, stillbirth, prematurity, postmaturity, intrauterine development retardation and in the newborns' cord sera who had congenital anomalies like anencephaly, cataract and dolichocephaly were investigated. in these mothers hsv-1 igg positivity ratio was 71/73 (97.3%). there was no significant difference in the age group distribution of hsv-1 igg ... | 1993 | 8264443 |
| the proportion of herpes simplex virus-specific cytotoxic t lymphocytes (tc) that recognize glycoprotein c varies between individual mice and is dependent on the form of immunization. | in mice the immune response to hsv-1 includes a brisk tc response that is intimately associated with the control of infection. this report evaluated the tc response to gc, one of the envelope glycoproteins of hsv-1. this protein was recognized as a target antigen for tc from hsv-1 immune mice only if they expressed the h-2kb mhc allele. however, even within these "responder" strains of mice the proportion of gc specific tc was highly variable. the failure of hsv-induced tc to recognize gc in the ... | 1993 | 8386515 |
| efficacy of the herpes simplex virus types 1 and 2 mutant viruses to confer protection against zosteriform spread in mice. | two mutant viruses, hsv-2 xd192 and hsv-1 1716, failed to generate zosteriform lesions when injected in high dose into balb/c and c3h mice. mice exposed to mutant viruses were solidly immune to challenge by wild-type homologous or heterologous virus. however, at lower immunizing doses protection was evident against lethality, but not skin lesions, especially in the case of mutant xd192. protection could be conferred with lymphoid cells from mutant virus immune mice and again, protection against ... | 1993 | 8386516 |
| [acyclovir-resistant herpes simplex viruses in hiv-infected patients]. | 1993 | 8290931 | |
| phosphorylation of neurotropic alphaherpesvirus envelope glycoproteins: herpes simplex virus type 2 ge2 and pseudorabies virus gi. | i have examined the state of phosphorylation of the envelope glycoproteins of two neurotropic herpesviruses, hsv-2 and prv. hsv-2 ge2 and prv gi, which are the homologues to hsv-1 ge, were found to be phosphorylated and phosphoaminoacid analysis revealed that both contained phosphoserine. these findings are consistent with a conserved phosphorylation of the glycoprotein homologues to hsv ge among the neurotropic alphaherpesviruses. | 1993 | 8391184 |
| latent infection with the ms strain of herpes simplex virus type 2 in the mouse following intracerebral inoculation. | intracerebral inoculation of the ms strain of herpes simplex virus type 2 (hsv-2) into mice causes an acute encephalitis associated with multifocal demyelination and necrotizing retinitis. we have studied the distribution of latent virus in mice that had recovered from the acute encephalitis. four weeks or longer after inoculation, hsv-2 could be recovered from the trigeminal ganglia of all mice examined by co-culture of explants in roller tubes. the virus could not be recovered from explants of ... | 1993 | 8289071 |
| risk of acquisition of genital herpes simplex virus type 2 in sex partners of persons with genital herpes: a prospective couple study. | in a prospective study of factors associated with genital herpes simplex virus (hsv) type 2 transmission in couples in whom 1 partner had clinical genital hsv and the other did not, 11 (19%) of the 57 history-negative partners had hsv-2 antibody by western blot at entry. in follow-up (mean, 16 months) of 29 hsv-2-seronegative partners, overall 4 (14%) seroconverted compared with 3 (23%) of 13 hsv-1- and -2-seronegative partners and 1 (6%) of 16 hsv-1-seropositive partners. since all seroconverte ... | 1993 | 8383724 |
| differential mechanism of cytostatic effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine, 9-(1,3-dihydroxy-2-propoxymethyl)guanine, and other antiherpetic drugs on tumor cells transfected by the thymidine kinase gene of herpes simplex virus type 1 or type 2. | after they have been transfected with the herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) thymidine kinase (tk) gene murine mammary carcinoma (fm3a) cells become highly sensitive to the growth inhibitory properties of the antiherpetic agents (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu), 9(-)[(2-hydroxyethoxy)methyl]guanine (acyclovir, acv), 9(-)[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, ganciclovir), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau). bvdu was 100-f ... | 1993 | 8384209 |
| herpes simplex virus type-2 infection by a footpad route results in neuronal death in mouse spinal ganglia. | we have examined the effects of herpes simplex virus type 2 (hsv-2) infection on neuron numbers in mouse dorsal root ganglia (drg) following unilateral hind footpad inoculation. one month following hsv-2 strain ms inoculation, tissue sections of decalcified spine containing the paired 4th and 5th lumbar drgs were stained with cresyl violet. neuronal numbers, somal areas and ganglion volumes were determined for ganglia ipsilateral and contralateral to both hsv-2 and medium inoculations. one month ... | 1993 | 8387099 |
| antiviral effect of oligo(nucleoside methylphosphonates) complementary to the herpes simplex virus type 1 immediate early mrnas 4 and 5. | we have previously shown that an oligo(nucleoside methylphosphonate) (deoxynucleoside methylphosphonate residues in italics) complementary to the acceptor splice junction of herpes simplex virus type 1 (hsv-1) immediate-early (ie) pre-mrnas 4,5 [d(tptcctcctgcgg)], causes sequence-specific inhibition of virus growth in infected cell cultures (smith et al., 1986; kulka et al., 1989). here we report a similar inhibition of hsv-1 growth by oligo(nucleoside methylphosphonates) complementary to the sp ... | 1993 | 8384823 |
| encephalitis in immunocompetent patients due to herpes simplex virus type 1 or 2 as determined by type-specific polymerase chain reaction and antibody assays of cerebrospinal fluid. | a herpes simplex virus type 2 (hsv 2) etiology was sought in 93 consecutive cases of herpes simplex encephalitis (hse) in immunocompetent post neonate patients. antibodies to hsv 2 glycoprotein g antigen were determined by an enzyme-linked immunosorbent assay (elisa) and hsv 2 dna in cerebrospinal fluid (csf) by a nested polymerase chain reaction (pcr) assay with primer pairs in the glycoprotein g gene. evidence of hsv 2 infection was found in 6 patients; hsv 2 dna was demonstrated in csf and th ... | 1993 | 8385702 |
| analysis of a herpes simplex virus 2 fragment from the open reading frame of the large subunit of ribonucleotide reductase with transcriptional regulatory activity. | expression of herpes simplex virus 2 (hsv-2)-encoded ribonucleotide reductase (rr) is required for growth in nondividing cells. the functional enzyme is composed of a large and a small subunit. in virus-infected cells, rr is expressed temporally as a delayed early protein. however, the promoter regulatory region of the large subunit can function as an immediate early promoter in transient transfection assays, suggesting that expression may be quite complex. in this study, a 95-bp fragment derive ... | 1993 | 8385955 |
| herpes simplex virus infection of human fibroblasts and keratinocytes inhibits recognition by cloned cd8+ cytotoxic t lymphocytes. | cd8+ cytotoxic t lymphocytes (ctl) clones with specificity for herpes simplex virus (hsv) were derived from two donors with genital hsv-2 infection. these ctl clones specifically lysed hsv-infected autologous b lymphoblastoid cells, but not hsv-infected fibroblasts. exogenous peptide loading sensitized both cell types to lysis by an hsv-specific ctl clone of known specificity. hsv infection rendered fibroblasts refractory to peptide sensitization. hsv infection also rendered fibroblasts and kera ... | 1993 | 8383706 |
| human papillomavirus, herpes simplex virus and other potential risk factors for cervical cancer in a high-risk area (greenland) and a low-risk area (denmark)--a second look. | the prevalence of human papillomavirus (hpv) infection and other risk factors were studied in a high risk area for cervical cancer (greenland) and in a low risk area (denmark). from nuuk (greenland) and nykøbing falster (denmark), random samples of 150 women aged 20-39 years were drawn. a total of 129 and 126 women were included in greenland and denmark, respectively. the proportion of hpv infected women assessed by virapap was similar in denmark and greenland (4.8 vs 3.9%). when type specific p ... | 1993 | 8385982 |
| the reliability of serological tests for the diagnosis of genital herpes: a critique. | the reliability and limitations of the currently used routine tests for herpes simplex virus type 2 (hsv-2) antibody in australia are reviewed. six case reports illustrate the clinical dangers of overinterpretation of the currently available kits and the need for a readily available specific hsv-2 antibody test. in sydney, hsv-2 causes approximately 85% of primary genital herpes and > 95% of recurrent genital herpes. due to the extensive serological cross-reactivity between hsv-1 and hsv-2, curr ... | 1993 | 8396232 |
| antiviral properties of the seed extract of an indian medicinal plant, pongamia pinnata, linn., against herpes simplex viruses: in-vitro studies on vero cells. | pongamia pinnata, linn., an indian medicinal plant used in the ayurvedha and siddha traditional medicine systems, for treatment of clinical lesions of skin and genitalia, was evaluated for antiviral properties against herpes simplex virus type-1 (hsv-1) and type-2 (hsv-2) by in-vitro studies in vero cells. a crude aqueous seed extract of p. pinnata completely inhibited the growth of hsv-1 and hsv-2 at concentrations of 1 and 20 mg/ml (w/v), respectively, as shown by complete absence of cytopathi ... | 1993 | 8386251 |
| resistance of herpes simplex virus type 2 to neomycin maps to the n-terminal portion of glycoprotein c. | entry of herpes simplex virus (hsv) into cells is believed to be mediated by specific binding of envelope proteins to a cellular receptor. neomycin specifically blocks this initial step in infection by hsv-1 but not hsv-2. resistance of hsv-2 to this compound maps to a region of the genome encoding glycoprotein c (gc-2). we have studied the function of gc-2 in the initial interaction of the virus with the host cell, using hsv-2 mutants deleted for gc-2 and gc-2-rescued recombinants. resistance t ... | 1993 | 8386261 |
| in vitro antiviral activity of polyoxomolybdates. mechanism of inhibitory effect of pm-104 (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o on human immunodeficiency virus type 1. | a screening for inhibitors of human immunodeficiency virus type 1 (hiv-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of hiv-1 in cd4+ human mt-4 cells. a novel heteropolyoxomolybdate named pm-104 with the chemical formula (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o was found to be associated with potent anti-hiv-1 activity. pm-104 interferes with virus infection at a very early step such ... | 1993 | 8387260 |
| sequence analysis of herpes simplex virus gb gene homologs of two platyrrhine monkey alpha-herpesviruses. | homologs of the herpes simplex virus gb gene were identified in two alpha-herpesviruses of platyrrhine monkeys, herpesvirus saimiri 1 (hvs 1) and h. ateles 1 (hva 1). these genes were cloned and sequenced in their entirety. analysis of the predicted amino acid sequences indicated that the gb glycoproteins of these two viruses are of similar size, have 10 cys residues and 5 potential n-linked glycosylation sites which align exactly with those in other primate alpha-herpesvirus gb polypeptides, an ... | 1993 | 8385913 |
| synthesis and antiviral activity of novel isonucleoside analogs. | a series of branched-chain sugar isonucleosides was synthesized and evaluated for antiviral activity against herpesviruses. the preparation of homochiral [3s-(3 alpha, 4 beta, 5 alpha)]-2-amino-1, 9-dihydro-9-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-6h-purin-6-one (7, bms-181,164) and related compounds was stereospecifically achieved starting from 1,2-isopropylidene-d-xylofuranose (10). an efficient two-step reduction of the anomeric center of bis-acetate 18 involved formation of the chlori ... | 1993 | 8387600 |
| nucleotide sequence of the major dna-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1. | the nucleotide sequence of a region encompassing about 5,200 base pairs (bp) of the left side of the origin of replication in the long unique region of the herpes simplex virus type 2 (hsv-2) has been determined. this region contained the major dna-binding protein or the infected-cell protein 8 (icp 8) gene and 5'-part of the counterpart of hsv-1 icp 18.5 gene. a comparison of the nucleotide sequence of the icp8 gene between hsv-1 and hsv-2 showed an 89.8% homology. a primer extension analysis f ... | 1993 | 8385914 |
| herpes simplex virus type 2 antibody in patients attending antenatal or std clinics. | to determine the prevalence of antibody to herpes simplex virus type 2 (hsv-2) in patients attending a general public antenatal clinic and three public sexually transmitted disease (std) clinics in sydney. | 1993 | 8387628 |
| molecular analysis of a neurovirulent herpes simplex virus type 2 strain with reduced thymidine kinase activity. | thymidine kinase (tk) of herpes simplex virus (hsv) has been identified as one of the factors responsible for its virulence. we have previously isolated acyclovir (acv)-resistant hsv type 2 (hsv-2), strain ys-4 c-1, by simple plaque cloning from a clinical isolate. although ys-4 c-1 had extremely low tk activity, it retained high virulence in mice. to determine the mechanism of the reduction of tk activity, a molecular analysis of the ys-4 c-1 tk gene was performed. ys-4 c-1 produced tk mrna, wh ... | 1993 | 8392322 |
| autocrine secretion of interferon-alpha/beta and tumour necrosis factor-alpha synergistically activates mouse macrophages after infection with herpes simplex virus type 2. | resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is strongly dependent on the function of macrophages (m phi). infection of mouse m phi with hsv-2 results in an early (4 to 10 h) activation of the cells with an enhanced respiratory burst generated after membrane triggering with a phorbol ester. the role of monokines produced during this infection was analysed. both interferon-alpha/beta (ifn-alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) were produced within the ... | 1993 | 8409942 |
| characterization of human keratinocytes transformed by high risk human papillomavirus types 16 or 18 and herpes simplex virus type 2. | recent reports implicate two dna tumour viruses, herpes simplex virus type 2 (hsv-2) and human papillomavirus types 16 or 18 (hpv-16 and -18), in the pathogenesis of cervical cancer. previous studies have indicated that primary human fibroblasts transfected with hpv-16 and hsv-2 morphological transforming region iii (mtr iii) are more aneuploid than fibroblasts immortalized with hpv-16 and that hsv-2 dna sequences are retained in transformed cells. since hsv-2 and hpv typically infect cells of e ... | 1993 | 8389810 |
| guanylylation and adenylylation of the alpha regulatory proteins of herpes simplex virus require a viral beta or gamma function. | herpes simplex virus genes form several groups whose expression is coordinately regulated and sequentially ordered in a cascade fashion. most of the products of the first group, the alpha genes, appear to have regulatory functions. we report that the alpha proteins, infected cell proteins 4, 0, 22, and 27 of herpes simplex virus 1 and 4, 0, and 27 of herpes simplex virus 2, were labeled in the isolated nuclei of infected hela cells with [alpha-32p]gtp or [alpha-32p]atp late in infection and that ... | 1993 | 8389911 |
| herpesvirus infections of the cns: mr findings. | herpesvirus infections of the cns are prevalent in all segments of the population, and are an important health care issue. the family of herpesviruses consists of a large group of double-stranded dna viruses that includes herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), cytomegalovirus, epstein-barr virus, varicella-zoster virus, b virus, herpesvirus 6, and herpesvirus 7. in addition to producing infection when the host initially acquires the virus, an important property ... | 1993 | 8390790 |
| [clinical and pharmacologic review of antiviral agents]. | the author presents a review if pharmacokinetics, undesirable effects, drug interactions, dosage, indications and drug forms of antiviral chemotherapeutic agents used for systemic treatment: amantadine and rimantadine (influenza virus a), acyclovir (hsv-1, hsv-2 and vzv), ganciclovir (cmv), zidovudine (hiv), ribavirine (broad spectrum) and fosfocarnet (herpes viruses and hiv), mentioning also vidarabine. all mentioned preparations are at least in some countries commercially available. the author ... | 1993 | 8435845 |
| orientation of the cleavage site of the herpes simplex virus glycoprotein g-2. | during the synthesis of glycoprotein g-2 (gg-2) of herpes simplex virus type 2, the 104,000-da gg-2 precursor (104k precursor) is cleaved to generate the 72k and the 31k intermediates. the 72k product is processed to generate the mature gg-2 (molecular mass, 108,000 da), while the 31k product is additionally processed and secreted into the extracellular medium as the 34k component (h. k. su, r. eberle, and r. j. courtney, j. virol. 61:1735-1737, 1987). in this study, the orientations of the 31k ... | 1993 | 8386284 |
| induction and enhancement of immune responses to herpes simplex virus type 2 in humans by use of a recombinant glycoprotein d vaccine. | a vaccine for a chronic or recurrent viral infection should induce immune responses that protect against primary disease or that augment preexisting defenses sufficiently to diminish the likelihood of disease recurrence or progression. such a vaccine was sought for genital herpes, a sexually transmitted infection of epidemic proportion. vaccine containing recombinant herpes simplex virus type 2 glycoprotein d expressed in cho cells was given repeatedly and safely to 24 human volunteers. in previ ... | 1993 | 8387560 |
| kinetic studies of the predicted substrate-binding site of varicella-zoster virus thymidine kinase. | to investigate the mechanism of kinetic action and substrate recognition of varicella-zoster virus (vzv) thymidine kinase (tk), we designed and isolated a site-directed mutant vzv tk which has double amino acid substitutions, 136threonine to leucine and 137isoleucine to leucine (sdm tk). this mutant was designed to alter the substrate-binding site of the vzv tk to duplicate that of the herpes simplex virus type 2 enzyme. kinetic studies of the activity of wild-type tk indicated that the binding ... | 1993 | 8389797 |
| oral acyclovir and recurrent genital herpes during late pregnancy. | to assess plasma acyclovir levels in pregnant women given oral acyclovir during late gestation and to determine the role and effect of oral acyclovir on asymptomatic shedding of virus in cases of recurrent genital herpes. | 1993 | 8390630 |
| detection of herpesviruses by polymerase chain reaction in lymphocytes from patients with rheumatoid arthritis. | to investigate the occurrence of herpesviruses, including epstein-barr virus (ebv), herpes simplex viruses types 1 and 2 (hsv-1; hsv-2), and human herpesvirus 6 (hhv-6), in lymphocytes from patients with rheumatoid arthritis (ra) of less than 1 year's duration. | 1993 | 8343184 |
| human papillomaviruses and other influences on survival from cervical cancer in panama. | to determine the influence on survival from cervical cancer of human papillomaviruses (hpvs) and other factors including age, herpes simplex virus type 2 (hsv-2) antibody status, and number of pregnancies. | 1993 | 8380103 |
| [demonstration of herpes simplex virus (hsv)-2 igg and igm using elisa in transsexuals and homosexuals]. | in this study, the sera of 12 cases of transsexual with vaginoplasty and 15 cases of homosexual have been investigated with elisa from the view point of hsv-2 igg and igm antibodies. twenty-five cases were within the 18-26 age groups and the other 2 transsexuals were 36 years old. our studying group have no significant clinical pathology. in all the 27 cases of transsexual and homosexual have been found to be positive for hsv-2 igg. in 7 cases, hsv-2 igm have been found positive, the other 20 ca ... | 1993 | 8380622 |
| herpes laryngitis and tracheitis causing respiratory distress in a neonate. | a newborn presented 1 week after birth with the sudden onset of stridor and respiratory distress. at endoscopy, the supraglottic and subglottic tissues were edematous and erythematous, and numerous plaquelike ulcers were seen on the mucosal surface. cultures of the lesions yielded herpes simplex virus type 2. the infant required a prolonged course of acyclovir and remained intubated for 31 days, but ultimately had both virological and clinical cure. evaluation and further treatment of this unusu ... | 1993 | 8381288 |
| hla-unrestricted killing of hsv-1-infected mononuclear cells. involvement of either gamma/delta+ or alpha/beta+ human cytotoxic t lymphocytes. | the characterization of hsv-specific human ctl, obtained in short term cultures by stimulating pbmc of healthy hsv-immune donors with autologous, pha-activated, hsv-1-infected mononuclear cells, is described. ctl induced by using this technique are able to mediate a strong lytic activity against both hsv-1- and hsv-2-infected targets, whereas they do not kill autologous ebv-lymphoblastoid cell lines unless they are superinfected with hsv-1. tcr-gamma/delta+ cells are mainly responsible for hsv-s ... | 1993 | 8381835 |
| herpes simplex virus infection in pregnancy. | genital infections in pregnancy caused by herpes simplex virus types 1 and 2 are a difficult management problem. both primary and recurrent genital herpes can range from severe, extensive ulceration to asymptomatic virus shedding. although neonatal herpes is a well recognized complication of symptomatic maternal primary genital infection at the time of delivery, most cases are associated with asymptomatic virus shedding and absence of a history of genital herpes. neonatal herpes may also be acqu ... | 1993 | 8390339 |