| structural features of ribonucleotide reductase. | herpes simplex virus type 1 (hsv-1) encodes a ribonucleotide reductase which comprises two polypeptides with sizes of 136,000 (rr1) and 38,000 mol. wt. (rr2). we have determined the entire dna sequence specifying hsv-1 rr1 and have identified two adjacent open reading frames in varicella-zoster virus (vzv) which have homology to hsv rr1 and rr2; the predicted sizes for the vzv rr1 and rr2 polypeptides are 87,000 and 35,000 mol. wt. respectively. amino acid comparisons with rr1 and rr2 polypeptid ... | 1986 | 2835765 |
| anal cancer in women. | we studied predisposing factors in 56 women with anal cancer, comparing them with 56 matched controls drawn from the population. a detailed pretested questionnaire was administered to each study subject in a structured interview and blood was drawn for detection of herpes simplex virus antibodies by radioimmunoassay. pathologic material from cases was obtained and evidence of human papilloma virus infection was sought. by univariate analyses we found associations between anal cancer and positive ... | 1988 | 2836255 |
| a test for the relative potency of herpes simplex virus vaccines based upon the female guinea-pig model of hsv 2 genital infection. | an elisa for total herpes simplex virus (hsv) 1 antigen content and a test of immunogenicity based upon the female guinea-pig model of hsv 2 genital infection were applied to two samples from batches of hsv 1 subunit ('skinner') vaccine. the elisa was reproducible within an approximately threefold limit of error and batches 1 and 2 were indistinguishable in antigen content. the effects of vaccination in the guinea-pig model were assessed by a statistical analysis of scores derived from the princ ... | 1988 | 2836425 |
| transmission of genital herpes in couples with one symptomatic and one asymptomatic partner: a prospective study. | to determine the risk of transmission of genital herpes simplex virus (hsv) infection, we prospectively studied, for a median of six months, 38 couples who had been together for a median of 10 mo. in each couple, one partner had a history of symptomatic genital herpes and one did not. at entry, of the 38 asymptomatic, exposed partners, 21 were seronegative, and results of western blot analysis showed that seven had antibody to hsv type 1 (hsv-1), four to hsv type 2 (hsv-2), and six to both hsv-1 ... | 1988 | 2836518 |
| antibody to herpes simplex virus (hsv) icp-35 proteins after hsv challenge of animals immunized with hsv subunit vaccines. | the serological response to the icp-35 group of herpes simplex virus type 2 (hsv-2) proteins was examined 30 d after vaginal challenge with hsv-2 of guinea pigs previously immunized with hsv glycoproteins. detection of antibodies to icp-35 proteins correlated best with the magnitude of vaginal viral replication. animals that developed antibody to icp-35 proteins had more-frequent recurrences (p less than .0002) that were of longer duration (p less than .005) than animals that did not. by covaria ... | 1988 | 2836519 |
| antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of uv b-irradiated mice. | uv b-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (hsv-2) induced suppressor t-cell circuits that decreased hsv-2-induced proliferative responses of hsv-2-immune lymph node cells. adoptive transfer experiments indicated that splenocytes from uv b-irradiated hsv-2-infected animals contain l3t4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. however, following in vitro culture of the splenocy ... | 1988 | 2836632 |
| monoclonal antibodies directed against a 130k glycoprotein of bovine herpesvirus 2 cross-react with glycoprotein b of herpes simplex virus. | envelope proteins of bovine herpesvirus type 2 (bhv-2) and herpes simplex virus types 1 and 2 (hsv-1, -2) share cross-reacting determinants. monoclonal antibodies directed against epitopes of a 130k glycoprotein of bhv-2 detect determinants on the surface of infected cells and react either with cytoplasmic or nuclear antigens. biological and biochemical characterization of the proteins recognized by these antibodies revealed that the bhv-2 specific 130k glycoprotein and the gb of hsv share commo ... | 1988 | 2837013 |
| incorporation into nucleic acids of the antiherpes guanosine analog buciclovir, and effects on dna and protein synthesis. | using cells expressing herpes simplex virus (hsv) thymidine kinase, we investigated the metabolism of the acyclic antiherpes guanosine analog buciclovir, in relation to the effects of the drug on viral dna and protein synthesis. in these cells the predominant metabolite of buciclovir was its triphosphate, as in the hsv-1 infected vero cells investigated in parallel. further metabolism of buciclovir led to incorporation into rna and dna. buciclovir inhibited dna synthesis, not rna synthesis, and ... | 1988 | 2837218 |
| stress-related immune suppression: health implications. | this study used a year-long prospective design to assess linkages among distress, immunity, and illness. serial blood samples were collected from 40 first-year medical students at the first, third, and fifth examination periods, as well as 1 month before each. there were significant decrements in the production of gamma-interferon by concanavalin a-stimulated lymphocytes obtained at the time of examinations. antibody titers to epstein-barr virus (ebv) increased during examination periods, sugges ... | 1987 | 2837297 |
| prevalence of herpes simplex virus type 2 antibodies in selected population groups in italy. | the estimated prevalence of herpes simplex virus type-2 specific antibodies was less then 0.1% in a national sample of 1,169 healthy young males, 4.8% among 411 health care workers and 55% among 397 homosexuals. these viral infections seem to pose a health problem for risk groups such as homosexuals but not for the general population. | 1988 | 2837392 |
| class-specific herpes simplex virus antibodies in sera and cervical secretions from patients with cervical neoplasia: a multi-group comparison. | serum and cervical secretions were collected from patients with cervical dysplasia, carcinoma-in-situ (cis), squamous cell carcinoma (cervical scc), and controls with normal cervices, attending clinics within the west lambeth health district, london. enzyme-linked immunosorbent assays were used to examine cervical secretory iga (siga) and serum igg and iga antibodies to herpes simplex virus (hsv). sexual and demographic factors were considered during data analysis, which involved fitting multipl ... | 1988 | 2837405 |
| suppression of humoral immune response against herpes simplex virus induced by defective strains, ts- and tk- mutants. | suppression of humoral antibody formation against hsv is not only induced by replicating herpes simplex virus type 2 (hsv-2) but also by the defective strain ang and the deletion mutant 1301 of herpes simplex virus type 1 (hsv-1). moreover, ts-mutants a, h, k, s, 1201 and 1208 of hsv-1 as well as some ts-mutants of hsv-2 and "defective-interfering" particles of hsv-1 after high multiplicity of infection-passages induced suppression. treatment of infected mice with acg reduced antibody-formation ... | 1988 | 2835944 |
| antibodies to simian vacuolating virus 40 in bladder cancer patients. | the occurrence of antibodies to herpes simplex virus type 2 (hsv-2) and simian vacuolating virus 40 (sv40) (two presumptive oncogenic viruses) was investigated by the technique of enzyme-linked immunosorbent assay in 233 patients with bladder cancer (200 males and 33 females) and in 466 controls (400 males and 66 females). the age of both the bladder cancer patients and controls varied from 55 to 65 years. a statistically significant association between bladder cancer and antibodies to sv40 was ... | 1987 | 2834853 |
| regulation of viral and cellular genes in a human neuroblastoma cell line latently infected with herpes simplex virus type 2. | a latent state of the herpes simplex virus type 2 genome was established in a human neuroblastoma cell line (sms-kcnr) to initiate studies on the mechanism by which host cells interact and regulate latent viral genes. to establish viral latency, it was necessary to prevent virus replication by briefly exposing the infected cells to antiherpetic acycloguanosine (20 microm) and human interferon (120 u/ml). subsequently however, these cells could be propagated without any antiherpetic agents and al ... | 1988 | 2838126 |
| interaction of polylysine with the cellular receptor for herpes simplex virus type 1. | we earlier reported that neomycin blocked reversibly the binding of herpes simplex virus type 1 (hsv-1) to the receptor of bhk cells, while the binding of hsv-2 to the receptor was unaffected. we could not determine whether the effect was on the virus particle, the receptor, or both. we have now tested several other cationic substances, and report that polylysine (and polyarginine) block the binding of hsv-1 to the receptor by interfering with the cellular receptor function; higher molecular wei ... | 1988 | 2838567 |
| effect of herpes simplex virus type 2 infection on mitochondrial gene expression. | a cdna clone derived from the mitochondrial cytochrome oxidase i gene has been used to show that the level of mitochondrially encoded rna species declines during herpes simplex virus type 2 infection in a manner similar to that for rna species derived from nuclear genes. in contrast to the situation for nuclear genes, however, no change in the transcription rate of the mitochondrial genome during infection was detected, indicating that post-transcriptional processes alone are responsible for the ... | 1988 | 2838578 |
| inhibition of herpes simplex virus type 2 replication in vitro by 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a. | the in vitro antiviral activity as well as the mechanism of action of a new antiviral agent, a kanamycin analogue, 1-n-pentadecanoyl-3''-n-trifluoroacetyl kanamycin a (ptka) against herpes simplex virus type 2 (hsv-2) was investigated. the drug showed excellent antiviral action with negligible cytotoxic effect on the culture cells. based on plaque reduction assays the 50% inhibitory dose (id50) of the drug was 1 microgram/ml, and at 20 micrograms/ml plaque formation was totally suppressed. the c ... | 1988 | 2839107 |
| purification of the herpes simplex virus type 1 65-kilodalton dna-binding protein: properties of the protein and evidence of its association with the virus-encoded dna polymerase. | using a combination of conventional column chromatography and velocity sedimentation, we have purified the 65-kilodalton dna-binding protein (65kdbp) encoded by herpes simplex virus (hsv) greater than 625-fold. the hsv type 1 (hsv-1)-encoded dna polymerase (pol) cofractionated with 65kdbp through deae-sephacel, blue sepharose, and mono q columns and was only separated from 65kdbp by sedimentation through a glycerol gradient. immunoaffinity columns containing monoclonal antibody (mab) 6898 immuno ... | 1988 | 2839706 |
| changing patterns of genital herpes simplex virus infections. | herpes simplex virus type 2 is considered by many venereologists as the predominant serotype among those patients suffering from genital herpes. however, identification of genital isolates obtained from 16 through 20, and 31 through 40 year old individuals among our female patient population shows a predominance of hsv-1 infections. these data suggest that the "above the waist"-"below the waist" epidemiologic patterns commonly ascribed to reflect a prevalence of hsv-1 and hsv-2 infections respec ... | 1988 | 2839940 |
| methyl gallate, methyl-3,4,5-trihydroxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. i. purification and characterization of methyl gallate from sapium sebiferum. | a potent anti-herpetic compound was identified and purified to homogeneity from the leaves of sapium sebiferum by plaque reduction assay using herpes simplex virus type 2. the chemical structure of the purified compound was determined by mass spectroscopy and proton and carbon-13 nuclear magnetic resonance as methyl gallate, methyl-3,4,5-trihydroxybenzoate. this is the first demonstration that methyl gallate is a potent anti-herpetic compound in vitro, present in high concentration in the leaves ... | 1988 | 2840132 |
| methyl gallate, methyl-3,4,5-trihydoxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. ii. antiviral activity of methyl gallate and its derivatives. | methyl gallate (mg), methyl-3,4,5-trihydroxybenzoate, was highly active against herpes viruses as determined by plaque reduction assay. herpes simplex virus type 2, ms strain, was sensitive to mg at a mean 50% inhibitory concentration (ic50) of 0.224 micrograms/ml in monkey kidney cells. mg was specific for herpes viruses with the relative sensitivity hsv-2 greater than hsv-1 greater than cmv. two rna viruses tested were significantly less sensitive to mg. the structural components of mg which m ... | 1988 | 2840133 |
| detection of herpes simplex virus type 2 dna in uterine cervix lesions using cloned bgl ii n fragment of hsv-2 dna as a probe. | | 1988 | 2840390 |
| effect of macrophage activation on resistance of mouse peritoneal macrophages to infection with herpes simplex virus types 1 and 2. | to define the effect of heterogeneity of murine peritoneal macrophages (m phi) on intrinsic resistance to herpes simplex virus (hsv) infection, several m phi populations were characterized for their response to infection with hsv type 1 (hsv-1) and hsv-2. steady-state resident m phi (res m phi) were compared in parallel with m phi activated with corynebacterium parvum (now designated propionibacterium acnes) (cp m phi) and thioglycollate-elicited inflammatory m phi (tg m phi). res m phi were com ... | 1988 | 2841412 |
| specific induction of cellular gene transcription in herpes simplex virus type 2-transformed cells. | in spite of much work, the mechanism of oncogenic transformation by herpes simplex virus (hsv) is as yet unknown. it has been proposed that hsv type 2 (hsv-2) can transform cells by a 'hit and run' mechanism. in the past we have demonstrated that several polypeptides can be immunoprecipitated from hsv-2-transformed cells, but not from control cells or adenovirus-transformed cells, by rabbit hyperimmune sera to hsv-2. it is possible that the expression of these proteins might be the result of act ... | 1988 | 2841413 |
| a cellular protein related to heat-shock protein 90 accumulates during herpes simplex virus infection and is overexpressed in transformed cells. | a monoclonal antibody produced from mice immunized with hsv-infected cell dna binding proteins reacts with two cell-encoded polypeptides, p90 and p40, synthesized constitutively by many different cell types and expressed at high levels in transformed cells. in uninfected cells heat shock induces a cytoplasmic accumulation of p90 and in agreement with this we show that p90 is related to hsp90. during hsv-2 lytic productive infection, p90 accumulates to very high levels, such accumulation being de ... | 1988 | 2842174 |
| in vivo behavior of genetically engineered herpes simplex viruses r7017 and r7020: construction and evaluation in rodents. | the herpes simplex virus (hsv) recombinant r7017 was constructed from hsv-1 (strain f) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the l component with a fragment of the hsv-2 genome encoding the glycoproteins g, d, i, and a portion of e. in addition, the r7020 recombinant contains an hsv-1 dna fragment encoding the tk gene fused to the alpha 4 gene promoter. the results of studies in mice ... | 1988 | 2842408 |
| history of recurrent mucocutaneous herpes correlates with relatively low interferon production by herpes simplex virus-exposed cultured monocytes. | monocytes separated from blood specimens of healthy voluntary blood donors were infected with herpes simplex virus (hsv) 1 or 2. the amount of interferon produced by the hsv-exposed monocytes was found to be donor-specific and was similar whether tested with hsv 1 or hsv 2. the interferon levels in repeated blood specimens from a given donor were relatively stable within an observation period of a few months. the survey was expanded by testing 27 persons with history of recurrent herpes disease ... | 1988 | 2842448 |
| phosphonoformate and phosphonoacetate derivatives of 5-substituted 2'-deoxyuridines: synthesis and antiviral activity. | the synthesis of potential "combined prodrugs" wherein phosphonoformate or phosphonoacetate was attached to the 5'-position of 2'-deoxyuridine, 2'-deoxythymidine, 5-iodo-2'-deoxyuridine (idu), 5-(2-chloroethyl)-2'-deoxyuridine (cedu), or 5-(2-bromovinyl)-2'-deoxyuridine (bvdu) or to the 3'-position of cedu is described. the antiviral activities of these derivatives and of reference compounds were compared in vero, hep-2, and primary rabbit kidney cells against herpes simplex virus types 1 and 2 ... | 1988 | 2842506 |
| evaluation of macrophage antiviral activity in patients affected by neoplasia. | the intrinsic antiviral activity of macrophages has been studied in healthy donors and in patients affected by breast cancer and melanoma. in vitro differentiated macrophages from blood-derived monocytes were infected with measles virus, herpes simplex virus type 2 and adenovirus 17. the challenge was carried out with different multiplicities of infection and the synthesis of virus was tested by evaluating the single cycle growth curve in 24 h. the results obtained show that the restriction of v ... | 1988 | 2842553 |
| increased efficacy of phosphonoformate and phosphonoacetate inhibition of herpes simplex virus type 2 replication by encapsulation in liposomes. | phosphonoformate and phosphonoacetate encapsulated in liposomes have substantially greater activity against herpes simplex virus type 2 in vero cell tissue culture than the nonencapsulated compounds at the same dose. encapsulation of phosphonoformate in liposomes resulted in a 30-fold increase of the antiviral effect with no increase in cytotoxicity measured by inhibition of thymidine incorporation into normal vero cells. thus, the selectivity of the liposomal drug increased 27-fold compared wit ... | 1988 | 2843083 |
| inducible expression of herpes simplex virus type 2 glycoprotein gene gg-2 in a mammalian cell line. | the gg-2 glycoprotein gene of herpes simplex virus type 2 (hsv-2) was cloned into the mammalian expression vector pmsg under the control of the inducible mouse mammary tumor virus promoter. transfection of this cloned gg-2 construct into nih 3t3 cells resulted in the stable expression of gg-2 upon induction with dexamethasone. in addition, the 104,000-molecular-weight (104k) and 72k gg-2 precursors as well as the 34k secreted component were generated in the transformed cells. the synthesis of gg ... | 1988 | 2843667 |
| placebo-controlled study with subunit herpes simplex virus vaccine in subjects suffering from frequent herpetic recurrences. | the safety and efficacy of a subunit herpes simplex virus (hsv) type 1 vaccine were tested in a small-scale double-blind trial carried out in a group of 42 volunteers suffering from frequent recurrences of herpetic lesions. the patients were paired according to sex, age, type of virus isolated, previous history of the disease and some non-specific immunological markers. one member of each pair received repeated doses of hsv vaccine, the other a placebo. clinical reactions were mild. antibody res ... | 1988 | 2844031 |
| effectiveness of a procedure for disinfecting the vaginal photoplethysmograph contaminated with herpes simplex virus type 2. | to test the effectiveness of a disinfecting procedure involving 2% glutaraldehyde, a vaginal photoplethysmograph was contaminated with a known amount of herpes simplex type 2 (hsv-2). the vaginal photoplethysmograph was then put through the disinfection procedure. two virus solutions were tested, one designed to approximate the concentration found in a naturally occurring infection (low inoculum), the other with 100 times more virus (high inoculum). varying lengths of exposure to glutaraldehyde ... | 1988 | 2844137 |
| multicentric intraepithelial neoplasia involving the vulva. clinical features and association with human papillomavirus and herpes simplex virus. | sixteen of 46 patients (35%) with grade 3 vulvar intraepithelial neoplasia (vin 3) were found to have an additional site of lower genital tract squamous cell neoplasia, primarily in the cervix. the frequency of multicentricity decreased significantly with age. in addition, patients with multicentric disease (involving the vagina and/or cervix in addition to the vulva) had a significantly higher frequency of multifocal disease involving the vulva (involving more than one location on the vulva) an ... | 1988 | 2844383 |
| infection of human thymic epithelial cells by human cytomegalovirus and other viruses: effect on secretion of interleukin 1-like activity. | cultures of human thymic epithelial (te) cells are able to produce a interleukin 1 (il-1) like activity. this il-1 activity can be detected either using mouse thymocytes in a traditional il-1 assay, or using thymic lymphocytes obtained from cases of pediatric cardio-vascular surgery. production of il-1 activity by te cells was found to be maximal between 3 and 4 weeks after culture initiation. human thymocytes worked best as targets in an il-1 assay, when these cells were derived from donors you ... | 1988 | 2844452 |
| herpes simplex virus ii antigen in adenocarcinoma of the uterine cervix. | | 1988 | 2844663 |
| mutational analysis of the herpes simplex virus type 1 trans-inducing factor vmw65. | the herpes simplex virus type 1 (hsv-1) polypeptide vmw65 is a structural component of the virus particle and is also responsible for trans-induction of immediate early (ie) transcription. functional domains of this polypeptide were investigated by constructing a series of 10 plasmids each with a 12 bp insertion in the gene encoding vmw65. plasmids were analysed for their ability to stimulate ie transcription in short term transfection assays, and the altered vmw65 polypeptides were assayed for ... | 1988 | 2844968 |
| detection of herpes simplex virus type 1 in herpetic ocular diseases by dna-dna hybridization using a biotinylated dna probe. | a diagnostic hybridization assay for detecting herpes simplex virus type 1 (hsv-1) in ocular specimens was developed using cloned viral dna as a probe. this hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target dna by a streptavidin/alkaline phosphatase system. the time required for performing this assay system is only two days. this assay system could detect a probe which had been hybridized to as little as 1 pg of homologous dna and did not cross-rea ... | 1988 | 2844977 |
| c3b receptor activity on transfected cells expressing glycoprotein c of herpes simplex virus types 1 and 2. | glycoprotein c from herpes simplex virus type 1 (gc-1 from hsv-1) acts as a receptor for the c3b fragment of the third component of complement on hsv-1-infected cell surfaces. direct binding assays with purified gc-1 and c3b demonstrate that other viral and cellular proteins are not required for this interaction. although c3b receptor activity is not expressed on hsv-2-infected cell surfaces, purified gc-2 specifically binds c3b in direct binding assays, suggesting that gc-1 and gc-2 are functio ... | 1988 | 2845122 |
| anti hsv-2 properties of amniotic fluid. | some antiviral properties of amniotic fluid (af) were studied. a close relationship between maternal blood and af antiviral antibodies was seen. substances other than antibodies which could have an effect on viruses were assumed to be present in af. using hsv2 as a model, we found a virus-neutralizing activity in 6 out of 7 specimens studied which does not appear to be related to antibodies. | 1988 | 2845231 |
| modification of the genital herpes infection in a guinea pig model, by prior immunization with bovine herpes mammillitis virus. | bovine herpes mammillitis virus has been shown to partially protect guinea pigs against primary genital herpes simplex virus type 2 infections, further confirming the immunologic cross-reactivity of these two viruses. | 1988 | 2845232 |
| inhibition of terminal n- and o-glycosylation specific for herpesvirus-infected cells: mechanism of an inhibitor of sugar nucleotide transport across golgi membranes. | the nucleoside analog (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) inhibited the golgi-associated terminal glycosylation in herpes simplex virus type 1- and type 2-infected cells, specifically incorporation of galactose and sialic acid into n-linked oligosaccharides, and incorporation of sialic acid and, to a lesser extent, of galactose into o-linked oligo saccharides. this resulted in formation of viral glycoproteins with terminal glcnac and fuc in n-linked oligosaccharides and terminal o-linked ... | 1988 | 2845656 |
| an acyclovir-resistant strain of herpes simplex virus type 2 which is highly virulent for mice. | herpes simplex virus type 2 (hsv-2), strain ys-4 c-1, isolated by plaque cloning from a clinical isolate was found to be resistant to acyclovir (acv; acycloguanosine) in vitro. it was sensitive to phosphonoacetic acid and 9-beta-d-arabinofuranosyladenine. thymidine kinase (tk) activity of ys-4 c-1 was less than 1% of that of other strains from the same clinical source. however, thymidine plaque autoradiography showed that ys-4 c-1 was not completely deficient in tk activity. ys-4 c-1 showed high ... | 1988 | 2845890 |
| induction of cervical intraepithelial neoplasia in balb/c mice by uv-inactivated herpes simplex virus type 2. | | 1988 | 2845906 |
| influence of sera from patients affected by neoplasia on some human macrophage functions. | it has been repeatedly reported that several functions of mononuclear cells are impaired in patients affected by neoplasia. moreover, inhibitory activity of serum and tumor extracts on macrophages have been described. in a previous study, we found a marked impairment of the intrinsic antiviral activity of macrophages derived from monocytes isolated from peripheral blood of patients with breast carcinoma or melanoma compared with that from blood of normal subjects. the aim of the present work was ... | 1988 | 2846174 |
| efficacy of recombinant glycoprotein d subunit vaccines on the development of primary, recurrent, and latent genital infections with herpes simplex virus type 2 in guinea pigs. | vaccination of guinea pigs with recombinant glycoprotein d (subunit vaccine) from either herpes simplex virus type 1 or type 2 (hsv-1, hsv-2), before intravaginal inoculation with hsv-2, markedly altered viral replication in the vaginal tract and reduced, or completely prevented, the development and spread of external genital lesions. vaccination with glycoprotein d from hsv-2 prevented the establishment of latent hsv-2 infections in dorsal root ganglia and reduced the frequency of recurrent epi ... | 1988 | 2834467 |
| hypomethylation of host cell dna synthesized after infection or transformation of cells by herpes simplex virus. | infection of rat embryo cells with herpes simplex virus type 2 caused undermethylation of host cell dna synthesized during infection. dna made prior to infection was not demethylated, but some of its degradation products, including methyl dcmp, were incorporated into viral dna. the use of mutant virus showed that some viral dna synthesis appears to be required for the inhibition of methylation. inhibition of methylation cannot be explained by an absence of dna methyltransferase as the activity o ... | 1988 | 2837642 |
| [prevention of lymphatic and hematologic spreading as a pathogenic condition for neural persistence of herpes simplex virus]. | in the experimental model of genital herpes simplex virus (hsv) infection of the mouse--in spite of abundant virus replication on the mucous membranes--no infectious virus can be isolated from the inflamed and swollen draining lymph nodes (dln), contrary to the positive results in the lumbosacral nerves and their associated ganglia. attempts to reactivate an abortive infection possibly established in lymph node or spleen cells by stimulation with phytohemagglutinin or lipopolysaccharide rendered ... | 1988 | 2837877 |
| paget's disease of the vulva: search for herpes simplex virus antigens and human papillomavirus antigen and dna. | specimens from three women with paget's disease of the vulva were tested for presence of herpes simplex virus type 2 (hsv2) antigens and human papillomavirus (hpv) antigen and dna. in one of the lesions, the hsv2-associated antigen icsp 34/35 was demonstrated. neither hpv antigens nor hpv dna were detected in the lesions. | 1988 | 2846413 |
| on the control of immediate early (alpha) mrna survival in cells infected with herpes simplex virus. | the alpha or immediate early mrna of herpes simplex virus strain hsv-2(g) had a half-life of about 15 min if made in the absence of viral protein synthesis but was relatively stable if viral protein synthesis occurred, either freely or restricted by the presence of the proline analogue azetidine. in contrast, the alpha mrna of other strains of the virus is stable, even in the absence of protein synthesis. studies with recombinant viruses showed that the region of the viral dna between 0.58 and 0 ... | 1988 | 2846763 |
| herpes simplex virus (hsv)-specific proliferative and cytotoxic t-cell responses in humans immunized with an hsv type 2 glycoprotein subunit vaccine. | studies were undertaken to determine whether immunization of humans with a herpes simplex virus type 2 (hsv-2) glycoprotein-subunit vaccine would result in the priming of both hsv-specific proliferating cells and cytotoxic t cells. peripheral blood lymphocytes (pbl) from all eight vaccines studied responded by proliferating after stimulation with hsv-2, hsv-1, and glycoprotein gb-1. the pbl of five of these eight vaccines proliferated following stimulation with gd-2, whereas stimulation with gd- ... | 1988 | 2846864 |
| herpes simplex viruses lacking glycoprotein d are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors. | herpes simplex virus (hsv) glycoprotein d (gd) plays an essential role in the entry of virus into cells. hsv mutants unable to express gd were constructed. the mutants can be propagated on vd60 cells, which supply the viruses with gd; however, virus particles lacking gd were produced in mutant-infected vero cells. virus particles with or without gd adsorbed to a large number (greater than 4 x 10(4] of sites on the cell surface; however, virions lacking gd did not enter cells. cells pretreated wi ... | 1988 | 2846873 |
| properties of two 5'-coterminal rnas transcribed part way and across the s component origin of dna synthesis of the herpes simplex virus 1 genome. | in cells infected with herpes simplex virus 1 the domains of the two identical origins of viral dna synthesis in the s component (oris) are transcribed in two 5'-coterminal rnas. orisrna1, synthesized early in infection and also in the absence of protein synthesis by infected cells, runs antisense to and terminates at the transcription initiation site for alpha genes 22 or 47. orisrna2, reported earlier, was detected in cells 14 hr after infection; it extends across the origin and is coterminal ... | 1988 | 2847162 |
| the cleavage recognition signal is contained within sequences surrounding an a-a junction in herpes simplex virus dna. | herpesvirus genome maturation involves site-specific cleavage of viral dna concatemers and encapsidation of unit-length molecules, processes that are apparently coupled. here, applying a transfection-infection approach, we have investigated the arrangement of the dna sequence elements involved in cleavage and shown that specific cleavage occurs independently of dna replication. we show that the cis-acting signal for cleavage is located within a 179-bp fragment from across an a-a junction formed ... | 1988 | 2847414 |
| combined herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid leads to oncogenic transformation. | tumorigenic transformation was produced by combining herpes simplex virus type 2 and human papillomavirus type 16 or 18 deoxyribonucleic acid (each is associated with cervical carcinoma). recombinant human papillomavirus type 16 and 18 deoxyribonucleic acid each containing a head-to-tail dimer of the full-length human papillomavirus respective genomes, induced morphologic transformation in an immortalized cell line (ae), obtained by transfection of syrian hamster embryo cells with immortalizing ... | 1988 | 2847531 |
| effects of various nucleosides on antiviral activity and metabolism of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil against herpes simplex virus types 1 and 2. | uptake of 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau) into herpes simplex virus type 1 (hsv-1)- and 2 (hsv-2)-infected cells was elevated about 190 to 40 times, compared with that into mock-infected human embryo lung fibroblast cells. uptake was not enhanced by infection with thymidine kinase-negative hsv-1 and hsv-2 mutants, however. in hsv-1-infected cells, 9.7% of bv-arau was phosphorylated to bv-arau triphosphate, but only 1.1% was phosphorylated in hsv-2-infected cells. the ... | 1988 | 2847634 |
| selective inhibition of human cytomegalovirus replication by a novel nucleoside, oxetanocin g. | a novel nucleoside with an oxetanosyl-n-glycoside has been recently isolated from a culture filtrate from bacillus megaterium and named oxetanocin a (n. shimada, s. hasegawa, t. harada, t. tomisawa, a. fujii, and t. takita, j. antibiot. 39:1623-1625, 1986). in this study, we evaluated the antiherpesvirus activity of oxetanocin a and its derivatives and found that 9-(2-deoxy-2-hydroxymethyl-beta-d-erythro-oxetanosyl)guanine (oxt-g) was very potent and selective in inhibiting the replication of hu ... | 1988 | 2847638 |
| correlation of histologic types of carcinoma of the uterine cervix and human papillomavirus and herpes simplex virus type 2 dna sequences in the uterine cervical biopsies. | forty patients with invasive cancer of cervix from new delhi were examined for the presence of human papillomavirus (hpv) and herpes simplex virus (hsv) type 2 dna sequences in a southern hybridization assay. data were analysed according to the histologic type of cancer. hpv dna sequences were detected in 82.5% biopsies and hsv-2 dna sequences were found in 10% biopsies. hpv-16 dna sequences were found either alone or together with hpv-18 and/or hsv-2 bgl ii n fragment in 55% biopsies from kerat ... | 1988 | 2848476 |
| human papillomavirus & herpes simplex virus type 2 dna sequences with chronic cervicitis & invasive cervical cancer. | | 1988 | 2848763 |
| ability of herpes simplex virus (hsv) types 1 and 2 to induce clinical disease and establish latency following previous genital infection with the heterologous hsv type. | guinea pigs were infected intravaginally with either herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2). one month postinoculation, animals were inoculated with the heterologous hsv type and observed for clinical disease and shedding of virus. one month after superinfection, animals were killed, and tissues were cocultivated to detect latent virus. although the severity of clinical disease and the degree of shedding of virus were greatly reduced by prior infection with the heterologous virus ... | 1988 | 2848899 |
| chronic-dose acyclovir to suppress frequently recurring genital herpes simplex virus infection: effect on antibody response to herpes simplex virus type 2 proteins. | to determine the effect of prolonged suppressive acyclovir therapy on the antibody response to herpes simplex virus type 2 (hsv-2) proteins, we studied sequential sera from 33 patients with frequently recurring (six or more recurrences per year) genital herpes. twenty-two patients received 400 mg of oral acyclovir and 11 received placebo, twice daily for one year. sera collected at enrollment, after six months and 12 months of therapy, and during the first recurrence after cessation of therapy w ... | 1988 | 2848900 |
| complement component c3b binds directly to purified glycoprotein c of herpes simplex virus types 1 and 2. | cells infected with herpes simplex virus type 1 (hsv-1), but not hsv-2, express on their surfaces a receptor for the complement component c3b. receptor activity is markedly enhanced by treatment of the infected cells with neuraminidase. employing a direct binding assay, consisting of purified hsv glycoproteins immobilized on nitrocellulose and iodinated c3b as a probe, we found that c3b binds directly to gc-1, as well as to gc-2, but not to gb or gd from either serotype. c3b binding was enhanced ... | 1987 | 2849025 |
| interaction of herpes simplex virus type 2 with a rat glioma cell line. | the interaction between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and two neural cell lines, mouse neuroblastoma (n1e-115) and rat glioma (c6-bu-1), was investigated. n1e-115 cells were permissive to both types of hsv. in c6-bu-1 cells, on the other hand, all the hsv-1 strains tested so far showed persistent infection, and the infectious virus of hsv-2 strains disappeared spontaneously. the hsv-2-infected c6-bu-1 cells were positive for hsv-2-specific dna sequences, virus-specific r ... | 1988 | 2850449 |
| herpes simplex virus induces the replication of foreign dna. | plasmids containing the simian virus 40 (sv40) dna replication origin and the large t gene are replicated efficiently in vero monkey cells but not in rabbit skin cells. efficient replication of the plasmids was observed in rabbit skin cells infected with herpes simplex virus type 1 (hsv-1) and hsv-2. the hsv-induced replication required the large t antigen and the sv40 replication origin. however, it produced concatemeric molecules resembling replicative intermediates of hsv dna and was sensitiv ... | 1988 | 2850486 |
| neurovirulence of individual plaque stocks of herpes simplex virus type 2 strain hg 52. | the virulence of ten independent plaque stocks of hsv-2 strain hg 52 was determined by intracranial inoculation of 3 week old balb/c mice. the ten stocks exhibited a range of ld50 which could be grouped into three classes; high (less than 10(3) pfu/mouse), intermediate (10(3)-10(4) pfu/mouse) and low (greater than 10(5) pfu/mouse). analysis of the ten stocks using six different restriction endonucleases showed no significant variation in the size and distribution of fragments. isolates from the ... | 1988 | 2850778 |
| electron microscopic localization of acridine orange chromatin interaction products in cells transformed by herpes simplex virus type 2. | the purpose of this study was to examine the distribution pattern of acridine orange (ao) binding to dna in transformed cells and derived tumor cells. two cell clones from hamster embryonic fibroblasts (hef) transformed by herpes simplex virus type 2, tumor cells derived from the transformed cell clones, and normal hef in culture were processed for ultracytochemistry. uptake studies and autoradiography using [3h] thymidine and [3h]-uridine were also performed. ultrastructural examination reveale ... | 1988 | 2851256 |
| presence of insulin binding sites on viral particles. | using a standard radio-receptor assay, we have demonstrated that [125i]insulin can bind specifically to each of two types of purified enveloped viruses, influenza a virus and rous sarcoma virus. a non-enveloped icosahedral virus (echovirus 11) and herpes simplex virus type 2, which acquires its envelope from the nuclear membrane of the cell, did not possess insulin receptor activity. displacement of specifically bound radiolabelled insulin from the viral surface was achieved by addition of an ex ... | 1988 | 2851292 |
| the use of colloidal gold immunoelectron microscopy to diagnose varicella-zoster virus (vzv) infections by rapid discrimination between vzv, hsv-1 and hsv-2. | colloidal gold immunoelectron microscopy was used to diagnose rapidly 53 cases clinically suspected of varicella-zoster virus (vzv) infection and one special case selected from another study on typical herpes simplex virus (hsv) infections. the viruses were identified and subsequently typed within 2.5 h by a direct labelling test for vzv, and within 3.5 h by an indirect labelling test with monoclonal antibodies against hsv type 1 and type 2. the protein a purified igg fraction of human anti-vzv ... | 1988 | 2851604 |
| secretory iga against herpes simplex virus in cervical secretions. | a recently developed method to recover proteins from cervical secretions was combined with methods to detect minute concentrations of herpes simplex virus (hsv) type specific antibodies to measure the concentrations of locally produced antibodies in women with genital infections. forty nine women attending a sexually transmitted disease (std) clinic were included. cervical secretions were obtained by suction into a plastic catheter. soluble proteins were recovered from the secretions by elution ... | 1988 | 2852157 |
| does neoplasia in-situ develop due to the interaction of epstein-barr virus or herpes simplex virus-2 with langerhans cells in the epithelium? | | 1988 | 2852413 |
| broad-spectrum antiviral activity of the acyclic guanosine phosphonate (r,s)-hpmpg. | (r,s)-9-(3-hydroxy-2-phosphonomethoxypropyl)guanine [(r,s)-hpmpg] exhibits broad spectrum antiviral activity with an ed50 of less than 1 microm against herpes simplex virus (hsv) types 1 and 2, varicella zoster virus, human cytomegalovirus (hcmv) and vaccinia in plaque reduction assays. wild type hsv-2 and its thymidine kinase deficient variant are equally sensitive to (r,s)-hpmpg. (r,s)-hpmpg is 100-fold more potent than acyclovir (ed50 = 0.45 microm vs. 44 microm, respectively) against hcmv in ... | 1988 | 2852486 |
| [seroepidemiological survey of herpes simplex virus type 2 in cervical erosion women]. | | 1988 | 2852541 |
| herpes simplex virus glycoprotein treatment of recurrent genital herpes reduces cervicovaginal virus shedding in guinea pigs. | treatment of previously infected guinea pigs with herpes simplex virus (hsv) glycoproteins reduces the frequency and severity of subsequent genital recurrences. an effective vaccine should also reduce episodes of viral shedding. in this study, hsv glycoproteins b and d treatment of animals experiencing recurrent genital herpes reduced the frequency of both clinical recurrences and cervicovaginal viral shedding. when virus was shed, however, the peak viral titer and duration of shedding was unalt ... | 1988 | 2852922 |
| homology of the hsv-2 "a-sequence" to cellular sequences. | bgl-ii fragments of the genome of herpes simplex virus type 2 (hsv-2) hg-52 were cloned into the vector p-neo and were used to screen the complete hsv-2 genome for regions cross-hybridizing with the genome of hel cells. most extensive cross-hybridizing activity was observed with a 530 bp sstii subfragment of the viral bamhi g dna-fragment (contained in bgl ii f), which spans the joint and the viral a-sequence. from a lambda-l47 library, a cellular 15 kb hindiii dna fragment was subcloned in pbr ... | 1988 | 2853486 |
| the aetiological relationship between herpes simplex virus type 2 and carcinoma of the cervix: an unanswered or unanswerable question? | after two decades of investigation the proposed aetiological link between herpes simplex virus type 2 (hsv2) and human carcinoma of the cervix remains unproved. the initial seroepidemiological studies, which were of a retrospective nature, were in favour of the aetiological relationship, whereas the more recent prospective studies suggest that hsv infection is a co-variable. the detection of hsv genes and their products in tumour tissues has been plagued by problems of cross reactivity, but the ... | 1988 | 2853997 |
| cervical carcinoma: an experimental approach. | a mouse model system was used to investigate the preventive efficacy of a subunit herpes simplex virus (hsv) vaccine on the development of hsv induced cervical carcinoma. ten groups of mice were vaccinated before receiving repeated intravaginal exposure to hsv-type 2 inactivated by ultraviolet irradiation. at 20 months postvaccination, neutralizing antibody activity to herpes simplex viruses was detected in the sera of the mice which had received the highest vaccine dose. although three experime ... | 1988 | 2853998 |
| dna sequence and rna transcription through a site of recombination in a non-neurovirulent herpes simplex virus intertypic recombinant. | re6 is a herpes simplex type-1 (hsv-1) x herpes simplex type-2 (hsv-2) intertypic recombinant that cannot replicate in the adult mouse nervous system. in the accompanying report, we have shown that hsv-1 sequences between 0.698 and 0.721 map units can restore a partial neurovirulent phenotype to re6. in this report, we have used comparative dna sequence analysis of re6, 17syn+ (hsv-1) and hg52 (hsv-2) to demonstrate that this region contains a site of recombination between hsv-1 and hsv-2 sequen ... | 1988 | 2854325 |
| vaccine potential of a live avirulent herpes simplex virus. | we previously described a herpes simplex intertypic recombinant that is completely and specifically non-neurovirulent following intracranial inoculation. in this report the vaccine potential of this mutant (re6) is analyzed. it is shown that re6 replicates to wild type levels at the peripheral site of inoculation (the mouse footpad) but, unlike its virulent parental strains, does not replicate to detectable levels in lumbosacral ganglia. a single vaccination with re6 is sufficient to completely ... | 1986 | 2854601 |
| antiviral activities of 2'-deoxyribofuranosyl and arabinofuranosyl analogs of sangivamycin against retro- and dna viruses. | eight sugar-modified pyrrolopyrimidine nucleoside analogs related to the antibiotic sangivamycin were evaluated in cell culture against herpes simplex types 1 (hsv-1) and 2 (hsv-2), cytomegalovirus (cmv), adenovirus, and visna virus. five of the compounds were highly active against most of the viruses with 50% inhibition (ed50) values of 1-10 microm. the selectivity of the agents was low, with inhibition of uninfected cell proliferation occurring within 5-fold that of the virus ed50 for most of ... | 1988 | 2854957 |
| antiherpes activity of the immunomodulator ok-432, a streptococcal preparation, in immunosuppressed mice. | the antiviral activity of ok-432, an antitumor agent originating from streptococcal preparations, against herpes simplex virus type 2 (hsv-2) was investigated in mice immunosuppressed by cyclophosphamide (cy). intraperitoneal administration of ok-432 to mice 1 day after treatment with 200 mg cy/kg prevented death due to hsv-2 encephalitis in a dose-dependent manner. when the immunosuppressed mice were given ok-432 prior to hsv-2 infection, both by the intraperitoneal route, virus growth in the p ... | 1988 | 2854959 |
| efficacy of 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) against herpes simplex virus type 2 strains in cell cultures and against experimental herpes encephalitis in mice: comparison with acyclovir and foscarnet. | the sensitivity of different herpes simplex virus type 2 (hsv-2) strains to inhibition by 5-vinyl-1-beta-d-arabinofuranosyluracil (varau) was evaluated in comparison to 9-(2-hydroxyethoxymethyl)guanine (acv; acyclovir) and trisodiumphosphonoformate (na3pfa; foscarnet), using a plaque inhibition assay in primary rabbit testes (prt) cells as well as in human embryonic lung fibroblast (helf) cell cultures. the order of decreasing activity found was acv much greater than varau greater than na3pfa in ... | 1987 | 2855541 |
| vaccine induced hsv 1 antibodies fail to correlate with protection against hsv 2 in guinea pigs. | guinea pigs immunised with hsv 1 subunit vaccine were bled, and subsequently challenged intravaginally with hsv 2. the clinical response to virus challenge was quantified, and correlations were sought between clinical score and virus-specific serum antibody titre in functional and binding assays (elisa, neutralization, complement-mediated cytotoxicity and antibody-dependent cellular cytotoxicity). no significant relationships were found, and it was concluded that reactivity in the serological as ... | 1988 | 2856207 |
| [herpes simplex virus type 2 antibody levels in sera from various age groups using elisa and ifat]. | we have investigated hsv-2, igg antibody levels in 180 sera which were obtained from 60 girl students between 18-20 years old, 60 pregnant women between 19-39 years old and 60 young men between 20-22 years old, by using elisa (enzyme linked immunosorbent assay) and ifat (indirect fluorescent antibody technique). all of the sera obtained from girl students and pregnant women were found negative for hsv-2, igg antibody by elisa. however 2 of 60 young men's sera were positive. totally 2 of 180 sera ... | 1988 | 2856217 |
| anti-herpes simplex virus and cytostatic activity of some new 5-substituted 1-(4-hydroxybutyl)-and 1-(2-hydroxyethoxymethyl) uracil nucleoside analogues. | two series of 5-substituted acyclic uracil nucleoside analogues (5-x-acyclo-u) were evaluated for their inhibitory effects against three herpes simplex virus type 1 (hsv-1) strains and one type 2 (hsv-2) strain in a plaque inhibition assay on human embryonic lung fibroblast (helf) cell cultures as well as for their ability to inhibit the proliferation of baby hamster kidney cells in suspension (bhk-s) culture. acyclovir [9-(2-hydroxyethoxymethyl)guanine; acv] and (s)-9-(2,3-dihydroxypropyl)adeni ... | 1985 | 2864819 |
| herpes simplex virus hepatitis in mice: effects of treatment with trisodium phosphonoformate. | clinical isolates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) induced focal necrotic hepatitis in icr/schö mice. the extent of the hepatitis could be considerably reduced by trisodium phosphonoformate (pfa) administered for 3 days in a daily dose of 400-600 mg/kg. the hsv hepatitis in mice could serve as a suitable model for testing antiherpetic drugs. | 1985 | 2869661 |
| induction of recurrent genital herpes simplex virus type 2 infection by ultraviolet light. | | 1986 | 2870285 |
| studies on interactions of dtk-hsv mutants with neurons in vitro. | thymidine kinase negative (dtk-) mutants of herpes simplex virus type 1 (hsv-1) multiplied well in rat brain glioma cells. a proportion (less than 1%) of glioma cells survived the infection with hsv and were designated "survivor" glioma cells. survivor cells of dtk- mutant virus infection ceased to produce infectious virus after two passages and were highly resistant to both hsv-1 and hsv-2 but not to vesicular stomatitis virus (vsv). flow cytometric studies indicated morphological differences b ... | 1986 | 2871727 |
| early intrathecal production of specific igm and igg antibodies and alpha-interferon in herpes simplex virus encephalitis. | a complex approach was used in order to establish non-invasively the aetiology in three cases of encephalitis presumptively caused by herpes simplex virus (hsv). as indicative of brain hsv infection was considered the lowered serum to cerebrospinal fluid specific antibody ratio, which also assessed the humoral immune response within the cns. for this purpose, during ongoing brain tissue infection, the early intrathecal (ith) production of igg and igm antibodies was analysed by a differential enz ... | 1986 | 2873733 |
| induction of chromosomal aberrations in human cells by a temperature-sensitive mutant of herpes simplex virus type 2 and its revertants. | the induction of chromosomal aberrations by a temperature-sensitive (ts) mutant of herpes simplex virus type 2 (hsv-2) strain hg52 (ts 13), its revertants 4-8 and 5-8 and by etalon strains hsv-1 17 syn+ and hsv-2 hg52 was studied in human fibroblast and lymphocyte cultures. the effect on chromosomes of the revertants was tested at permissive (31 degrees c) and non-permissive (38 degrees c) temperatures. at 38 degrees c the revertants could not induce dnase activity. the present results contribut ... | 1986 | 2874732 |
| comparative polypeptide analysis of human, murine and strigis herpesviruses with murine cytomegalovirus by polyacrylamide gel electrophoresis. | the polypeptide composition of five purified murine herpesvirus (mhv) strains grown in a stable line of rabbit embryo fibroblasts (ref) was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) and compared with herpes simplex virus type 1 (hsv-1). about 24 structural polypeptides of molecular mass ranging from 275,000 to 25,000 were identified in mhv and hsv-1. the polypeptide profiles of mhv and hsv-1, showed a close similarity. the polypeptides of mhv were further ... | 1987 | 2883851 |
| transcriptional induction of cellular gene expression during lytic infection with herpes simplex virus. | herpes simplex virus type 2 causes a severe repression of host cell biosynthesis at a number of levels. we show that despite this, non-viral cdna clones derived from cellular rna species which accumulate to high levels after infection can be isolated using differential screening techniques. by using nuclear run-off assays, we have shown that this rna accumulation is mediated by transcriptional induction of the corresponding cellular genes. | 1986 | 2883998 |
| studies of the major dna binding proteins of two bovine herpes mammillitis virus isolates. | antigenic cross-reaction of the dna-binding protein between herpes simplex virus type 2 (hsv-2) and two bovine herpes mammillitis virus (bhmv) isolates (bhm-1 and bhmv csiro 290) was demonstrated by indirect immunofluorescence. southern blot analysis showed a region of homology between hsv-2 bglii o dna (coding for the hsv-2 major dna-binding protein) and bhmv xbai dna fragment n, suggesting a similar gene map unit position on both genomes. | 1987 | 2886026 |
| high levels of cytomegalovirus antibody in patients requiring vascular surgery for atherosclerosis. | 157 caucasian male patients undergoing vascular surgery for atherosclerosis and a matched control group of patients with high cholesterol levels were screened for antibodies to cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv1) and type 2 (hsv2), indicative of persistent infection. the prevalence of cmv antibodies was higher in the surgical group than in the control group (90% and 74%, respectively), and a significantly greater percentage (p less than 0.001) of surgical cases than cont ... | 1987 | 2886763 |
| difference between herpes simplex virus type 1 and type 2 neonatal encephalitis in neurological outcome. | 24 infants consecutively treated with acyclovir or vidarabine for neonatal herpes simplex virus (hsv) encephalitis were followed up for 6 months to 3 years to assess neurological and developmental outcome. 15 patients had hsv-2 and 9 had hsv-1 encephalitis. infants with hsv-2 encephalitis presented with a higher frequency of seizures, greater pleocytosis and protein concentrations in the cerebrospinal fluid, and more frequent evidence of structural damage on computerised tomographic scans of the ... | 1988 | 2891886 |
| components required for in vitro cleavage and polyadenylation of eukaryotic mrna. | we have studied in vitro cleavage/polyadenylation of precursor rna containing herpes simplex virus type 2 poly a site sequences and have analyzed four rna/protein complexes which form during in vitro reactions. two complexes, a and b, form extremely rapidly and are then progressively replaced by a third complex, c which is produced following cleavage and polyadenylation of precursor rna. substitution of atp with cordycepin triphosphate prevents polyadenylation and the formation of complex c howe ... | 1988 | 2898767 |
| acyclic analogues of 3'-azido-3'-deoxythymidine as potential antiviral agents. nucleoside synthesis by michael addition. | an acyclic derivative of 3'-azido-3'-deoxythymidine, (r,s)-1-[1-(2-hydroxyethoxy)-3-azidopropyl]thymine (2), has been synthesized by a path involving michael-type addition. related thymidine analogues lacking the c(3')-c(4') bond were similarly obtained. the method provides a versatile new route to nucleoside analogues. the new compounds were found to be essentially inactive against human immunodeficiency virus type 1 (hiv-1) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro wh ... | 1989 | 2909745 |
| [viral or bacterial meningitis. differential diagnostic problems]. | a 19 year old previously healthy man presented symptoms of meningitis. culture from the cerebrospinal fluid revealed herpes simplex virus type 2 to be the responsible agent. the course was benign, the patient was never seriously ill. he recovered fully within a few weeks. the cerebrospinal fluid showed prolonged pleocytosis and low values of glucose. the traditional cerebrospinal fluid parameters employed in the diagnosis of meningitis, and for distinguishing bacterial from viral meningitis, are ... | 1989 | 2919375 |
| [herpes simplex virus infection of the central nervous system]. | herpes simplex virus type 1 (hsv 1) infection in the central nervous system results in encephalitis with high mortality and morbidity. however herpes simplex virus type 2 (hsv 2) infection in this area usually results in benign meningitis. brain biopsy is the most sensitive and most specific method for diagnosing hsv 1 encephalitis. antibody determinations in serum and cerebrospinal fluid give only a retrospective diagnosis. a rapid non-invasive diagnostic test is urgently required as an alterna ... | 1989 | 2919376 |
| antiviral activity of 5'-paa and 5'-pfa phosphate esters of 2'-deoxyuridines. | the 5'-paa and 5'-pfa phosphate esters of 5-bromo-2'-deoxyuridine (budr) were synthesized and their antiherpes virus activity was evaluated in vitro. both compounds showed activity of the same order as the parent nucleoside, budr, against hsv-2 but were 4 to 12 times less potent against hsv-1. the 5'-paa phosphate ester of budr (ro 21-9875) was also active against varicella-zoster virus (vzv) and human cytomegalovirus (hcmv). the 5'-paa phosphate ester of 5-bromovinyl-2'-deoxyuridine (bvdu) was ... | 1986 | 2947889 |
| a prospective study of association of herpes simplex virus and human papillomavirus infection with cervical neoplasia in women exposed to diethylstilbestrol in utero. | one thousand one hundred thirty-four women, enrolled in a prospective study of the development of cervical neoplasia in women exposed to diethylstilbestrol (des) in utero, were examined at least once a year. twenty-three women developed cervical intraepithelial neoplasia (cin) of different degrees during the 7-year follow-up period and were matched with women who did not develop cin during the study period. serum samples were obtained at the time of entry into the study and at the time of diagno ... | 1985 | 2981778 |