Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| transfection with the isolated herpes simplex virus thymidine kinase genes. ii. evidence for amplification of viral and adjacent cellular dna sequences. | we have previously described several series of biochemical transformation experiments in which small defined portions of herpes simplex virus (hsv-1 and hsv-2) dna encompassing the thymidine kinase (tk) gene were introduced into ltk- cells by the calcium transfection procedure. the presence of authentic virus tk enzyme in several subcloned cell lines derived from these experiments was confirmed by either the specific incorporation of [125i]iododeoxycytidine into their nuclei or the inhibition of ... | 1982 | 6286848 |
| clinical and virological features of herpes genitalis in japanese women. | we report herein clinical and virological findings in 35 japanese women with a confirmed herpes genitalis. the frequency of herpes genitalis was 0.24% in our obstetrical and gynecological outpatient clinic, while that in cancer screening clinics was 0.01%. eighty percent of all patients were between 20 and 49 years of age (median age of 38.5 years). genital herpes was detected more frequently in summer and autumn. virus isolations were carried out in 20 patients and 13 were positive (65.0%). of ... | 1982 | 6287795 |
| augmented immunogenicity of lewis lung carcinoma by infection with herpes simplex virus type 2. | in vitro, llt cells sustain hsv-2 replication without evidence of lysis. simultaneously, multiplication of the cells is stimulated. these xenogenized cells were tested for their immunopotentiating capacity: three-step immunization with xenogenized viable cells conferred significantly augmented transplantation resistance to a challenge graft with 4 x 10(4) intact llt cells. latency periods preceding tumor formation were increased and 15% of the mice failed to form primary tumors. metastasis was l ... | 1982 | 6288391 |
| animal studies on the efficacy of vaccination against recurrent herpes. | guinea pigs, latently infected with herpes simplex virus type 2 (hsv 2), were immunized with an hsv 2 vaccine derived from extracts of infected cells and either formol inactivated or detergent treated. although such postinfection vaccination could significantly increase the titers of neutralizing antibodies, it had no effect on the course of recurrent herpes in these animals. | 1982 | 6289061 |
| the effect of prostaglandins on the multiplication and cell-to-cell spread of herpes simplex virus type 2 in vitro. | herpes simplex virus type 2 (hsv-2) after infecting an individual has the ability to remain latent in nervous tissues. the factors that control herpesvirus infection, latency, and reactivation are poorly understood. fever, menstruation, emotional stress, exposure to sunlight, and surgical resection have been associated with activation of latent herpes. the situations which activate latent herpes are associated with a local or systemic rise in prostaglandins. the data presented show that prostagl ... | 1982 | 6289665 |
| protection against lethal challenge of balb/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. | monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gc, gab, gd, ge, and gf of herpes simplex virus type 2 were examined for their ability to protect balb/c mice from lethal infection by the virus. administration of monoclonal antibodies to individual glycoproteins intraperitoneally 3 h before footpad challenge with 10 times the 50% lethal dose of virus protected between 35 and 75% of the mice, except for one of two monoclonal antibodies recognizing ... | 1982 | 6290390 |
| early interactions of herpes simplex virus with mouse peritoneal macrophages. | adsorption of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to resident peritoneal macrophages (pm) of 4-week-old swiss albino (sa) and gr/afib mice was studied. a significantly (p less than 0.05) higher hsv-2 adsorption rate was found with pm of sa mice than with pm of gr/afib mice. of added hsv-2 65% bound to the cells of sa mice over a 120-min period versus 15% to pm of gr/afib mice. only 15 to 20% of added hsv-1 bound to pm regardless of the mouse strain. these patterns of adsorptio ... | 1982 | 6290397 |
| herpetic proctitis and meningitis: recovery of two strains of herpes simplex virus type 1 from cerebrospinal fluid. | a patient with simultaneous proctitis and meningitis due to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) was extensively investigated. in both disease locations the infection was clinically evident and culture-proven. analysis by sodium dodecylsulfate-polyacrylamide gel electrophoresis of rectal isolates revealed both hsv-1 and hsv-2. the cerebrospinal fluid harbored two apparently different strains of hsv-1, one of which was shown by restriction endonuclease analysis to be identical w ... | 1982 | 6290573 |
| properties of a herpes virus-transformed hamster cell line--i. growth and culture characteristics of sublines of high and low metastatic potential. | a herpes virus hominis type 2-induced tumour line of the syrian hamster, designated hsv-2-333-2-26, exhibited a low level of spontaneous metastasis from the primary subcutaneous tumour mass. two lung foci, met a and met b, were resected and transplanted s.c.; both lines showed an elevated metastatic potential compared with the parent cell line such that all animals inoculated with these cells developed metastatic disease within 40 days after resection of the primary tumour. neither the rapidity ... | 1982 | 6297914 |
| correlation of the virus sequence content and biological properties of cells carrying the herpes simplex virus type 2 thymidine kinase gene. | seven cell lines, transformed to a thymidine kinase-positive phenotype with herpes simplex virus type 2 (hsv-2) dna fragments, have been examined to determine their virus-specific dna sequence content. the results are consistent with the reported map position of the hsv-2 thymidine kinase gene. different cell lines contained different amounts of non-selected virus-specific sequences and this correlated with the ability of some cell lines to compensate the deficiencies in some temperature-sensiti ... | 1982 | 6292338 |
| transfection with the isolated herpes simplex virus thymidine kinase genes. i. minimal size of the active fragments from hsv-1 and hsv-2. | we have defined the minimal size and physical map locations in the genomes of both herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) for dna sequences capable of conferring stable biochemical transformation under thymidine kinase (tk) selection. the experiments involved transfection of ltk- cells with either isolated virus dna fragments or cloned pbr322 plasmids containing the 3.5 kilobase (kb) bamhi-o fragment from hsv-1(mp) or the 5.6 kb sali-g fragment from hsv-2(333). mapping of restricti ... | 1982 | 6292348 |
| isolation and characterisation of two strains of herpesvirus hominis type 1 from fallopian tubes. | herpesvirus hominis type 1 (hsv-1) was isolated from fallopian-tube biopsies of two women who were using an intra-uterine contraceptive device; the strains were isolated on different dates and at different hospitals. both the isolates were shown to be hsv-1 by restriction-enzyme analysis, polypeptide profiles and indirect immuno fluorescence; the two isolates could not be distinguished by these tests, but the tests distinguished them from hsv-1 strain 71-15 and hsv-2 strain 333. the viruses were ... | 1982 | 6292427 |
| glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies. | we used monoclonal antibodies reacting with glycoproteins specified by herpes simplex virus type 2 (hsv-2) to characterize the individual antigens in terms of structure, processing, and kinetics of synthesis in bhk or vero infected cells. our results provided a direct demonstration of the structural identity of the ga and gb proteins of hsv-2 as well as confirmation of the existence of type-specific and type-common domains within the gd molecule. they also show that, with the exception of gc, pr ... | 1982 | 6292496 |
| herpes simplex virus glycoprotein ga/b: evidence that the infected vero cell products comap and arise by proteolysis. | we recently reported (pereira et al., proc. natl. acad. sci. u.s.a. 78:5202-5206, 1981) that herpes simplex virus 1 and 2 glycoproteins, previously designated ga and gb, could not be differentiated by a bank of independently derived type-specific and type-common monoclonal antibodies. we also reported that from lysates of infected vero cells, all but one monoclonal antibody precipitated ga/b glycoproteins which had faster electrophoretic mobility than the corresponding infected hep-2 cell glycop ... | 1982 | 6292507 |
| unusual serologic response to two patients to an early antigen of herpes simplex virus type 2. | two patients infected with herpes simplex virus type 2 (hsv-2) who were free of recurrences over a 13-14-month period had an unusually high titer (greater than 1:48) of complement-fixing antibody to the early antigen (ag-4) of hsv-2. their response was also unusual in that it was an igg complement-fixing antibody rather than igm, which normally is found in patients with hsv-2 infection. the antibody was also present 13-14 months after the episode of hsv-2 disease, whereas in other patients, the ... | 1982 | 6293106 |
| [transformation and malignant degeneration of newborn hamster cells inoculated with herpes simplex virus type 2]. | 1982 | 6293203 | |
| comparison of the in vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine and its 5'-monophosphate. | in vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine 5'-monophosphate (ara-tmp) were compared with those of 1-beta-d-arabinofuranosylthymine (ara-t). on a molar basis ara-tmp was almost as active as ara-t against six strains of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) as monitored by a cytopathogenicity-inhibition and a plaque reduction assay in human embryonic lung fibroblast cells. when tested against experimental encephalitis in mice inoculated intrace ... | 1982 | 6293376 |
| comparison of restriction endonuclease profiles of dna from local herpes simplex virus type 2 (hsv-2) strains. | comparisons of restriction endonuclease profiles of dna isolated from nine genital hsv-2 strains isolated from 1973 to 1981 were made using six restriction endonucleases (bgl ii, hind iii, xba 1, eco ri, bam hi and hpa i). the only variation detected in the dna profiles was found with eco ri and bam hi, and in each case all the isolates could be classified into two groups. the difference between the two eco ri groups could be accounted for by the absence of an eco ri cleavage site from the hsv d ... | 1982 | 6293436 |
| chemical interactions with herpes simplex type 2 virus: enhancement of transformation by selected chemical carcinogens and pro-carcinogens. | quantitative assays for the morphological transformation of 3t3 swiss mouse cells by herpes simplex type 2 virus (hsv-2) were employed to examine the effect on cell transformation of chemical carcinogens and pro-carcinogens. the carcinogens tested were n-methyl-n'-nitro-n-nitrosoguanidine, quinacrine mustard, n-nitrosomethyl urea, urethane, and benzene. the pro-carcinogens tested were n-nitrosodimethylamine, 3-methylcholanthrene, benzo[a]pyrene, and p-dimethylaminoazobenzene. exposure of the cel ... | 1982 | 6293737 |
| [acquired immunologic deficiency syndrome, opportunistic infections and homosexuality. presentation of 3 cases studied in switzerland]. | three cases of multiple opportunistic infections in previously healthy homosexual males were observed in 3 different university hospitals in switzerland. two of the patients died. infections were multiple, with p. carinii pneumonia (3 cases), chronic mucocutaneous ulcers probably due to herpes simplex virus (2 cases), mucosal candidiasis (2 cases) and disseminated infections due to cytomegalovirus (1 case) and mycobacterium avium (1 case). all patients had depressed cellular immunity with marked ... | 1982 | 6294820 |
| chromosomal organization of the herpes simplex virus type 2 genome. | 1982 | 6294981 | |
| efficacy of (e)-5-(2-bromovinyl)-2'-deoxyuridine against different herpes simplex virus strains in cell culture and against experimental herpes encephalitis in mice. | (e)-5-(2-bromovinyl-2'-deoxyuridine (brvudr) showed strong antiviral activity against different laboratory strains and clinical isolates of herpes simplex virus type 1 (hsv-1) on primary rabbit testes (prt) cells with a 50% inhibition of plaque formation (id50) at 0.01-0.02 microm. one laboratory strain (hsv-1-s), however, was completely refractory even at concentrations as high as 100 microm. in contrast, the id50s for all herpes simplex virus type 2 (hsv-2) strains were about 10(2) - 10(3) tim ... | 1982 | 6295273 |
| restriction endonuclease analysis of dna from genital isolates of herpes simplex virus type 2. | high-resolution restriction endonuclease analysis of dna from multiple herpes simplex virus type 2 isolates from 30 patients at a single clinical center has indicated that herpes simplex virus type 2 dna sequences are highly conserved. however, the use of six endonucleases, ecori, bglii, hindiii, kpni, bamhi, and ssti, established that each patient was infected by a unique virus. comparison of virus isolates from the same patient show that the most sequence variation occurred in the terminal and ... | 1982 | 6295948 |
| virus-induced thymidine kinases as markers for typing herpes simplex viruses and for drug sensitivity assays. | a rapid, reproducible and objective new method for typing herpes simplex viruses type 1 (hsv-1) and type 2 (hsv-2) based on the effects of virus-induced thymidine kinases on various antiviral drugs has been developed. when several laboratory strains and clinical isolates were typed by this method and compared to the results obtained by the immunofluorescence antibody typing method, agreement was found for all the viruses. the new technique has the added advantage of determining the sensitivity o ... | 1982 | 6296179 |
| monoclonal antibodies to three non-glycosylated antigens of herpes simplex virus type 2. | 1982 | 6296279 | |
| neutralization of herpes simplex virus by antibody in amniotic fluid. | the ability of amniotic fluid to neutralize herpes simplex virus type 2 (hsv-2) was quantitated and compared with the serum neutralization titer in 158 pregnant women. neutralizing activity was expressed as the ability of 0.1 ml of amniotic fluid to reduce the expected number of plaque-forming units (pfu) in the inoculum by 99%. all amniotic fluid samples from 32 women with serum titers of 1:40 or greater neutralized 5 pfu or more; at term, 96% of these fluid samples neutralized 50 pfu or more, ... | 1982 | 6289206 |
| clinical and virologic studies on female genital herpes. | ninety women with genital herpes were studied clinically and virologically. from a clinical standpoint, these patients could be classified into 3 clinical types--acute, recurrent, and provoked. when herpes simplex virus (hsv) strains isolated from these patients and asymptomatic virus secretors were typed, 36 strains were determined to be type 1 (hsv-1) and the remaining 54 to be type 2 (hsv-2). the acute type was caused by primary infection of hsv-1 or hsv-2 as well as by infection of hsv-2 in ... | 1982 | 6289208 |
| [humoral immunity to herpes simplex virus type 2 in cervical cancer]. | the analysis of the results of serological studies revealed differences between the number of positively reacting sera from patients with cervical carcinoma and from normal women. the activity of complement-fixing and virus-neutralizing antibodies in the patients' sera and the frequency of their detection were higher than in the sera from normal subjects. these results in combination with other methods of laboratory examinations may be used for early detection of cervical carcinoma as well as fo ... | 1982 | 6289530 |
| inapparent genital herpes simplex virus infection in college women. | during a six-month period, 600 gynecological samples were collected from 585 women with typical herpes lesions, women with non-herpes symptoms (ie, vaginitis, moniliasis, trichomoniasis, etc), and normal women seen at the student health center gynecological clinic and processed for herpes simplex virus (hsv) isolation. from these specimens, 29 samples from 25 of the 585 women (4.3%) were positive for hsv. when these isolates were typed using plaque diameter in chick cells, heat stability of vira ... | 1982 | 6298359 |
| different weekly changes in immune response in virus-transformed cell-injected mice fed two different diets. | changes in tumor development and in certain immune responses were investigated at 7-weekly intervals after subcutaneous injection of 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells) into male balb/c mice fed 2 different diets. one diet contained 11% casein and 5% fat while the other had 11% supplemented wheat gluten and 30% fat. weanling mice (140/group) were fed one or the other of the diets for 12 weeks before injection and subsequent testing of 15 injected and 5 non-inject ... | 1982 | 6299512 |
| herpes virus-related antigens in herpes simplex virus type 2-transformed cells in the course of cervical carcinoma. | anticomplement immunofluorescence (acif) technique was applied to herpes simplex virus type 2 (hsv-2)-transformed cells (333-8-9) and to cells lytically infected with hsv-2 in sera from women with cervical carcinoma. there was a correlation between the positive results with both types of cells. long-term survivors suffering from cervical cancer showed a high percentage of positive immunofluorescence (if) reactions in hsv-2 transformed cells (77%) as well as in cells lytically infected with hsv-2 ... | 1982 | 6299751 |
| use of monoclonal antibodies to hsv-1 and hsv-2 for serological analysis of the viral glycoproteins. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) specify four major glycoproteins designated ga/gb, gc, gd and ge. type-specific and cross-reacting monoclonal antibodies to the viral glycoproteins have been used to probe their antigenic determinants. our studies show the following: (i) type-specific monoclonal antibodies are suitable reagents for serotyping isolates. discriminating monoclonal antibodies distinguish antigenic variants among the strains of each serotype. (ii) glycoproteins ga ... | 1982 | 6299824 |
| development of sensitive and enzyme-linked immunoassays for herpesvirus antigens and some applications. | sensitive enzyme-linked immunoassays for herpes simplex virus (hsv), cytomegalovirus (cmv) and varicella-zoster virus (vzv) were developed. both a sandwich technique, using antiviral antibodies from two species to detect the antigen, and an inhibition assay where the sample antigen was incubated with one antiserum, could be used. around 4-50 ng of viral antigens (measured as protein content) could be detected. the elisa inhibition technique using type-specific antisera could differentiate betwee ... | 1982 | 6299833 |
| application of molecular genetics to the design of live herpes simplex virus vaccines. | in principle, several avenues for attenuation of herpes simplex viruses (hsv) are now available. these include intermixing of hsv-1 and hsv-2 genes by recombination, altering the regulation of gene expression and the deletion of viral genes not required for replication of the virus in permissive cells in culture. results of analyses of hsv-1 x hsv-2 recombinants and of mutants containing a deletion in a gene expressed early in infection showed a loss of virulence when infected by intracerebral r ... | 1982 | 6299836 |
| herpes simplex virus subunit vaccine: characterization of the virus strain used and testing of the vaccine. | herpes simplex virus type 1 (hsv-1) used for vaccine production was isolated from a nasal recurrent infection and propagated over a limited number of passages in human diploid cells only. it was designated as hsv-1 bw3 and has been characterized by neutralizing antibodies as a typical hsv type 1 strain. in vitro transformation studies performed with this isolate in mouse or hamster cells revealed only very low, if any, transforming capacity. a preparation of hsv-1 bw3 which can be used as seed l ... | 1982 | 6299841 |
| intertypic superinfection of herpes simplex virus in vivo leads to reisolation of latent viruses with newly acquired lymphotropic properties. | the susceptibility of juvenile tupaias to several strains of temperature-sensitive mutants of hsv-1 and 2 was tested. it was found that in all cases the animal survived an infection of 1 x 10(7) pfu of temperature-sensitive mutants which were administered intravenously. in contrast, an infection dose of 1 x 10(3) pfu of wild-type hsv-2 and 1 x 10(2) pfu or hsv-1 was fatal for juvenile tupaias. those animals which had initially been infected with the ts-mutants of hsv-1 and/or 2 were protected ag ... | 1982 | 6299861 |
| the role of herpes simplex virus in cervical and vulvar cancer. | this report extends the association of herpes simplex virus type 2 (hsv2) with cervical cancer to vulvar carcinoma. in addition to seroepidemiologic observations linking prior hsv2 infection to genital cancer, the new evidence involves the neoplasms themselves. biopsies of cervical and vulvar cancers were found to contain hsv2-specified antigens. the virus-induced antigens are nonstructural dna-binding proteins normally present in the nuclei of infected cells; however, in the cancer cells they w ... | 1982 | 6299862 |
| characterization of an herpes simplex virus type 2 mutant, which is resistant to acycloguanosine and causes fusion of bsc1 cells. | a mutant of herpes simplex virus type 2, which induces low levels of thymidine-kinase activity in infected bsc1 cells and consequently able to grow in the presence of acycloguanosine, was isolated. this mutant has also been shown to cause fusion of bsc1 cells. in bsc1 cells, co-infected with the wild-type strain and the mutant, the yield of each of the two viruses was normal but the rounding and aggregation of cells observed, resembled that found in wild-type infected cultures. when the mixed in ... | 1982 | 6299232 |
| imide derivatives of 3-nitro-1.8-naphthalic acid: their inhibitory activity against dna viruses. | the antiviral action of a new drug, 5-amino-2-(dimethylaminoethyl)-benzo-[de]-isoquinolin-1.3-dione has been studied against herpes simplex type 2 (hsv-2) and adenovirus type 5 (ad-5) grown in vero cells. the concentration of the drug which gives a 5 log10 reduction in virus titer was 4 micrograms/ml (maximum tolerated concentration) for hsv-2. the anti-hsv-2 activity of this compound was one log. more powerful than that of iododeoxyuridine (idu). at this concentration the drug shows virucidal a ... | 1982 | 6299235 |
| abortive infection of neural cells by herpes simplex virus type 2. | the nature of the refractoriness of c6 rat glioma cells to herpes simplex virus type 2 (hsv-2) was examined. infection of c6 cells with hsv-2 results in low virus yields, not exceeding the input virus. although virus growth studies suggested a restricted cycle of virus replication, synthesis of hsv-2 dna and hsv-2-specific antigens could not be detected. in addition, hsv-2 yields in c6 cells were unaffected by interferon, cycloheximide, tunicamycin, actinomycin d and cytosine arabinoside. howeve ... | 1982 | 6299239 |
| modification of herpes 2-transformed cell-induced tumors in mice fed different sources of protein, fat and carbohydrate. | the effects of different sources of protein (milk, soy, wheat, fish and beef), fat (corn oil and butter), and carbohydrate (dextrin and sucrose) on tumor development and on spleen characteristics were investigated in balb/c mice injected subcutaneously with 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells). low or high levels of protein and fat were used. several weeks post-injection results indicated that a high level of fat significantly enhanced tumor incidence. a high fat ... | 1982 | 6299511 |
| modulation by cyclosporin a of murine natural resistance against herpes simplex virus infection. i. interference with the susceptibility to herpes simplex virus infection. | adult balb/c mice which are medium-high resistant against intraperitoneal (i.p.) infection with herpes simplex virus type 2 (hsv-2) manifested a drastic increase in susceptibility to the virus when treated locally with cyclosporin a (cya) during infection. oral application of the drug had no effect on the natural resistance status. mice appeared normal 2 weeks after cya treatment with regard to their ability to resist i.p. infections. cya did not interfere with established specific immune protec ... | 1982 | 6980627 |
| congenital herpes simplex virus, type 2, bilateral endophthalmitis. | a set of dizygotic twins, both born prematurely, developed herpes simplex encephalitis shortly after birth. the second twin had unilateral keratoconjunctivitis and bilateral endophthalmitis and subsequently died from disseminated herpes infection. herpes simplex, type 2, was isolated from conjunctiva of both eyes, cerebrospinal fluid, nasopharynx and trachea. histologic examination of the eyes revealed bilateral endophthalmitis with a necrotizing retinitis. intranuclear inclusion bodies were dem ... | 1982 | 6985228 |
| epidemiology of sexually transmitted diseases in the scandinavian countries. | in the scandinavian countries, laws require reporting of the traditional venereal diseases, i.e., gonorrhea, syphilis, lymphogranuloma venereum (lgv), and ulcus molle, or chancroid. incidence figures for these diseases, therefore, are fairly reliable. in the scandinavian countries, lgv and chancroid are rare diseases, and the syphilis incidence has remained low. these three diseases are typically male afflictions. gonorrhea outnumbers the other diseases mentioned and at present has a nearly 1:1 ... | 1982 | 6958011 |
| influenza type a virus-mediated adherence of type 1a group b streptococci to mouse tracheal tissue in vivo. | influenza type a virus-mediated adherence of pathogenic bacteria to the mucosal surface of the respiratory tract may be one of several mechanisms whereby influenza predisposes to bacterial pneumonia. in the present study, we quantified the adherence of intranasally administered type 1a group b streptococci to the tracheal tissue of influenza type a/pr8/34 (honi) virus-infected and mock-infected mice. influenza type a/pr8/34 virus infection effected a 120-fold increase in the adherence of type 1a ... | 1982 | 6754623 |
| treating viral venereal infections with the co2 laser. | viral venereal infections caused by herpes simplex virus 2 and papillomavirus (wart) are epidemic diseases in the united states. unfortunately, these two infections have eluded conventional therapy to date. therefore, 306 women were treated for viral venereal diseases with the co2 laser. two hundred twenty-eight patients were treated for condyloma acuminatum and 78 for genital herpes. all the patients who underwent treatment for herpes were enrolled in an investigative protocol. since the viral ... | 1982 | 6819358 |
| synergism among biolf-62, phosphonoformate, and other antiherpetic compounds. | 9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine (biolf-62) is highly synergistic with either phosphonoformate or phosphonoacetate when used in combination against herpes simplex virus types 1 and 2 in vitro. acycloguanosine did not show significant synergism with these two compounds. bromovinyldeoxyuridine and phosphonoformate were highly synergistic against herpes simplex virus type 2, but not against type 1. | 1982 | 6297382 |
| immunity to herpes simplex virus type 2. iv. impaired lymphokine production during recrudescence correlates with an imbalance in t lymphocyte subsets. | cell-mediated immunity (cmi) to herpes simplex virus type 2 (hsv-2) was studied in patients with recurrent disease. peripheral blood lymphocytes (pbl) obtained during recrudescence (0 to 3 days post-onset of lesion), convalescence (4 to 14 days), and quiescence (greater than 14 days), which were cultured in vitro in the presence of uv-inactivated hsv-2 (g) antigen, demonstrated similar, virus-specific lymphoproliferative responses. however, the production of the lymphokine, leukocyte migration-i ... | 1982 | 7045224 |
| spinal cord and optic nerve demyelination in experimental herpes simplex virus type 2 infection. | to produce a model of central nervous system (cns) demyelination, three strains of herpes simplex virus type 2 (hsv-2) were injected intracerebrally into swiss-webster outbred mice. animals developed pupillary abnormalities and leg weakness and, by light and electron microscopy, had focal lesions of primary demyelination in optic nerves and spinal cords. oligodendrocytes and astrocytes in these lesions contained herpesvirus. both mouse age and virus dose were important determinants of the rate a ... | 1982 | 7077361 |
| optimal treatment of herpes simplex virus encephalitis in mice with oral acyclovir. | the effect of oral or intraperitoneal administration of acyclovir was evaluated in four experimental models of herpes simplex virus (hsv) encephalitis in mice. mice were inoculated with hsv-1 or hsv-2 intracerebrally or with hsv-2 intranasally, intraperitoneally, or intravaginally. with all four routes of inoculation, oral acyclovir therapy significantly reduced mortality when started as late as 72 to 96 hours after viral challenge. intraperitoneal acyclovir was not effective in protecting mice ... | 1982 | 7102699 |
| virus-specific antibodies in sera from patients with genital herpes simplex virus infection. | virus-specific antibodies against a number of herpes simplex virus type 2 antigens were determined by radioimmunoprecipitation assays in sequential serum samples obtained from 12 patients with initial genital herpes simplex virus infection. the progressive appearance of antibodies to virus-specific antigens was observed; antibodies against a 130,000-molecular-weight glycoprotein complex appeared first, followed by antibodies against the major nucleocapsid polypeptide and then antibodies against ... | 1982 | 7118244 |
| herpes simplex type 2 virus encephalitis presenting as psychosis. | the current literature recognizes two antigenic types of herpes simplex virus, type 1 and 2. type 1 is the most common cause of sporadic necrotizing encephalitis in the united states, with a mortality rate of 30 to 70 percent, and leaves various neurologic sequelae in the survivors. herpes simplex virus type 2 had been recognized as an etiologic agent in fatal infections in neonates and a mild meningitis in adults, but its role in encephalitis in adults is less well known. we report a case of he ... | 1982 | 7124772 |
| lethal encephalitis and non-lethal multifocal central nervous system demyelination in herpes simplex virus type 2 infections in mice. | six-week-old swiss-webster mice were infected intracerebrally with a low dose of herpes simplex virus type 2 (hsv-2) and were studied by pathological, virological and immunological methods. one third of mice developed severe neurological disease and died during the first 31/2 weeks of infection, while the remaining two thirds survived this acute stage with relatively minor neurological signs. these survivors had multifocal demyelinative white-matter lesions in the cns, and gray-matter lesions, i ... | 1982 | 7150512 |
| aseptic meningitis and herpes simplex proctitis. a case report. | a male homosexual patient developed an aseptic meningitis during the course of acute ano-proctitis due to herpes simplex virus type 2 (hsv-2). aseptic meningitis (sometimes proved to be due to hsv-2) has been associated with genital but not with anal herpes. this sexually transmissible virus should be considered when patients with aseptic meningitis are investigated. | 1982 | 7198922 |
| epidural analgesia in the presence of herpes simplex virus (type 2) infection. | 1982 | 7201280 | |
| effect of phosphonoformate on symptomatic genital herpes simplex virus type 2 infection of guinea pigs. | using a guinea pig model of genital herpes simplex virus (hsv) type 2 infection, phosphonoformate treatment initiated after the onset of symptoms had a therapeutic effect on the outcome of disease. severity of disease, viral shedding, and hsv-induced cellular changes in vaginal cytology were significantly reduced in drug-treated animals as compared to sham-treated animals, although virus was readily detected in the nervous system of animals from both groups. the percentage of animals harboring l ... | 1983 | 6298142 |
| induction of a latent herpes simplex virus from a rat tumour initiated by herpes simplex virus-transformed cells. | a rat tumour induced by cells transformed with the sheared dna of herpes simplex virus (hsv) type 1 hfem alpha (re2a) was injected with the intertypic virus hsv-2 hg52 ts 1. separate plaques, isolated from cocultivation of excised tumour tissue with susceptible cells, yielded virus the dna of which had the restriction enzyme profile either of the injected hsv-2 virus or that of the hsv-1 virus, originally used to transform the cells. no evidence of in vivo recombination was detected. in hooded l ... | 1983 | 6298356 |
| dna sequence of the herpes simplex virus type 2 glycoprotein d gene. | we describe a 1635-bp herpes simplex virus type 2 (hsv-2) dna sequence containing the entire coding region of glycoprotein d (gd-2). the amino acid sequence of gd-2, deduced from the nucleotide sequence, was compared to that of the analogous herpes simplex virus type 1 (hsv-1) glycoprotein (gd-1). the two glycoproteins are 85% homologous and contain highly conserved regions of as much as 49 amino acids in length. comparison of dna sequences upstream from gd-1 and gd-2 coding regions identified p ... | 1983 | 6323270 |
| cell surface alterations induced by the acycloguanosine (acg) resistant and sensitive strains of herpes simplex virus type 2 (hsv-2) in the presence of acg and tunicamycin. | hsv-2-acg, a mutant of herpes simplex virus type 2, which is resistant to acycloguanosine and also capable of inducing fusion of bsc1 cells, was isolated. the viral-induced surface changes in these cells were studied using scanning electron microscopy. the formation of gigantic fused cells with increased number of microvilli and virus-like particles, extensively covering the infected cell surface, were investigated. tunicamycin (50 micrograms/ml), an inhibitor of glycosylation of polypeptides, b ... | 1983 | 6322282 |
| increase in laboratory-confirmed cases of herpes genitalis and neonatal herpes infections in israel. | jewish women have been considered to be at low risk for genital herpes simplex virus type 2 (hsv-2) infections. during the period 1973 to 1981, genital herpes simplex infections were laboratory confirmed in 129 cases (81 women and 48 men). until 1976, only sporadic cases were reported to our laboratory. since then, the number of cases has gradually increased, reaching 31 new reported cases in 1981. the age distribution was typical for a sexually transmitted disease, with the peak of infection at ... | 1983 | 6662679 |
| congenital absence of scalp skin and herpes simplex virus. a case report. | a male infant, born at 40 weeks' gestation of a 27-year-old mother, presented at delivery with three scalp lesions, 2 to 4 cm in diam, covered with necrotic tissue and devoid of hair. the infant was born by natural vertex delivery within 2 hours of membrane rupture; birth weight was 3,300 g and apgar score 10. there were no other skin lesions, and physical and neurological examinations were normal. herpes simplex virus type 2 was isolated from the lesions and the mother's cervix. high titers of ... | 1983 | 6662680 |
| [evaluation of 80 virologically-confirmed cases of ocular herpes. study of the correlation between the type of virus and the epidemiologic characteristics of the disease]. | 80 strains were isolated from patients with herpetic ocular infection during the period may 1979 to may 1981: 17 patients with herpetic blepharitis, 40 patients with superficial keratitis and 23 with deep stromal keratitis or kerato-uveitis. among 63 patients treated with antiviral agents, clinical resistance to the drug was observed in 17 cases. the strains were typed as hsv 1.1, 1.2 or 2.2 by seroneutralization and thermosensitivity in cellular cultures. no relationship between the viral types ... | 1983 | 6323567 |
| detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein c purified with monoclonal antibody. | herpes simplex virus type 1 (hsv-1) and hsv-2 specify at least four glycoproteins designated ga/gb, gc, gd, and ge. previous studies have shown that gc produced by hsv-1 is antigenically distinct from the corresponding hsv-2 glycoprotein. with the exception of gc, the glycoproteins of both serotypes share antigenic sites. standard serological assays fail to differentiate the antibody to the shared antigenic determinants from the type-specific antibody. in this paper, we describe a procedure for ... | 1983 | 6832831 |
| [possibilities and limits of chemotherapy of diseases caused by herpes simplex and varicella-zoster virus]. | the available antiviral chemotherapeutic agents for the treatment of herpes simplex type 1 and 2 and varicella zoster virus infections are discussed in respect of their mechanism of action, clinical effects, and side effects. although effective under experimental conditions most of the antiherpetic drugs need further evaluation of clinical efficacy in controlled trials. it is possible to reduce initial herpetic pain mainly in patients with zoster by topical application of 5% 5-iodo-2'-deoxyuridi ... | 1983 | 6841073 |
| protection of newborn mice against herpes simplex virus infection by prenatal and postnatal transmission of antibody. | pre- and postnatally acquired protection against herpes simplex virus type 2(hsv-2) infection mediated by maternal antibody was investigated in the newborn mouse. newborn mice, 2 days old, were inoculated with hsv-2 intraperitoneally after maternal immunization with live or inactivated virus. the survival rates improved in proportion to the maternal neutralizing antibody titres. ninety-three percent of animals delivered by caesarean section from immune mothers and suckled by non-immune mothers s ... | 1983 | 6842184 |
| leptomeningitis and polycaryocyte formation in the cns of rats inoculated subcutaneously with herpes simplex virus. | wistar rats were inoculated subcutaneously with either type 1 (hsv1) or type 2 (hsv2) herpes simplex virus at 5 days of age. animals were killed in extremis or at the end of the 14-day observation period postinoculation. acute destructive meningoencephalitis with hemorrhage and leukocytic infiltration was observed in both groups. polycaryocytes comprised of cells of the internal granular layer of the cerebellum were observed in some animals inoculated with hsv1. these multinucleated cells appear ... | 1983 | 6852211 |
| viruses and human cancer. | the cause of human cancer is probably multifactorial and the role of viruses is unclear. the study of retroviruses has led to the identification of oncogenes responsible for transformation and tumor induction. human viruses associated with malignancies include the jc virus (associated with progressive multifocal leukoencephalopathy) and some adenoviruses. no human malignancies have been associated with the latter group. a number of herpes viruses of lower animals have been associated with malign ... | 1983 | 6870184 |
| efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine against primary and recrudescent genital herpes simplex virus type 2 infections in guinea pigs. | the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) was evaluated for its efficacy in protecting guinea pigs from primary and recrudescent infections of herpes simplex virus type 2. vaginally infected guinea pigs were treated twice a day with dhpg at 25 mg/kg per dose for 3 weeks. subcutaneous doses were started 3 h, 24 h, or 5 weeks after virus inoculation. treatment starting at 3 or 24 h reduced the severity of the primary infection by greater than 70% when lesions were grad ... | 1983 | 6607031 |
| herpetic whitlow: report of a case with multiple recurrences. | a case of recurrent herpetic whitlow is discussed. the patient, a dental assistant at the time of her initial infection, developed herpetic whitlow caused by herpes simplex virus type 2 (hsv-2). she has subsequently experienced nine recurrent infections with lesion spread, scarring, and persistent paresthesia. the severity of the most recent recurrences appears to have diminished, and prodromal sensations, that is, increased local sensitivity, have occurred without the subsequent development of ... | 1983 | 6576289 |
| herpes type 2 infection with unusual generalised manifestations and delayed diagnosis in an adult male. | a case of severe generalised herpes simplex type 2 infection is described in an adult male who had known exposure to herpes. the patient first complained of headache, fever and neurological symptoms, and three to six days later of conjunctivitis, severe pharyngitis, arthralgia and vesicular lesions about the body. during the first 14 days of illness, including three in hospital, the patient was diagnosed as having infection with varicella virus, vesicular stomatitis virus, or hand-foot-and-mouth ... | 1983 | 6875292 |
| rapid diagnosis of varicella-zoster virus infection by detection of viral deoxythymidine kinase in serum and vesicle fluid. | a sensitive enzyme assay utilizing [125i]iododeoxyuridine as the substrate and ctp as the phosphate donor in combination with isozyme-specific antisera was used for direct detection and typing of herpesvirus deoxythymidine kinase (dtk) in clinical specimens. an investigation of 16 coded vesicle fluid specimens, taken in connection with varicella-zoster virus (vzv) and herpes simplex virus infections, revealed viral dtk activity in 14 samples. all positive samples except one were taken within 5 d ... | 1983 | 6339548 |
| current status and prospects for oral acyclovir treatment of first episode and recurrent genital herpes simplex virus. | herpes simplex genitalis is an increasingly common primary or recurrent hsv infection. acyclovir (acv) has been found to diminish virus shedding and clinical symptoms in patients with first episode genital hsv when given topically or intravenously. as most patients with first episode disease do not require hospitalization and recurrent disease is usually less severe than the primary infection, out-patient treatment forms the basis of practical management. acyclovir is absorbed orally with plasma ... | 1983 | 6355052 |
| lysis of herpesvirus-infected cells by macrophages activated with free or liposome-encapsulated lymphokine produced by a murine t cell hybridoma. | thioglycolate-induced mouse peritoneal macrophages were activated in vitro by the lymphokine designated macrophage-activating factor (maf) produced by a murine t cell hybridoma to lyse herpes simplex virus type 2 (hsv-2)-infected murine target cells. comparison of uninfected balb/c 10e2 cells with hsv-2-infected 10e2 cells showed that macrophages activated with maf selectively destroyed hsv-2-infected cells and left uninfected cells unharmed, as measured by an 18-h 51cr-release assay. in contras ... | 1983 | 6358037 |
| preferential inhibition of 5-trifluoromethyl-2'-deoxyuridine phosphorylation by 5'-amino-5'-deoxythymidine in uninfected versus herpes simplex virus-infected cells. | the cytotoxic effects of 5-trifluoromethyl-2'-deoxyuridine (cf3durd) were effectively antagonized by 5'-amino-5'-deoxythymidine (5'-adthd). the antiproliferative actions of cf3durd were reduced in a dose-dependent manner by 5'-adthd in both hela and vero cells. in addition, the ability of cf3durd to kill hela cells (95% at 1 microm and 99% at 3 microm), as measured by cloning efficiency, was ablated entirely by 5'-adthd (300 microm). in contrast, the inhibition of herpes simplex virus type 2 (hs ... | 1983 | 6408395 |
| differential susceptibility of human neural cell types in culture to infection with herpes simplex virus. | the ability of herpes simplex virus (hsv)-1 glasgow (strain 17), hsv-2 (strain hg52), temperature sensitive mutants and a thymidine kinase negative mutant of glasgow strain 17 to produce a cytopathic effect and induce viral antigen expression in primary and passaged human fetal brain cells was studied. no difference was detected between the behaviour of hsv-1 and hsv-2. cell-type-specific markers and indirect immunofluorescence were used to define unambiguously the type of individual cells in cu ... | 1983 | 6831193 |
| therapeutic activities of 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and -thymine alone and in combination with acyclovir and vidarabine in mice infected intracerebrally with herpes simplex virus. | the therapeutic effectiveness of two new antiviral agents, 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)thymine, was compared with that of acyclovir and vidarabine. in mice inoculated intracerebrally with high 50% lethal doses of herpes simplex virus type 2, nontoxic intraperitoneal or oral treatments with the two new fluorinated antiviral agents were highly effective in reducing mortality. the two drugs were also effective when trea ... | 1983 | 6625559 |
| topography of remyelinated chronic spinal cord lesions in herpes simplex virus type 2 infections of mice. | six-week-old outbred mice were infected intracerebrally with a low dose of the ms strain of herpes simplex virus type 2. about 1% of neurologically abnormal survivors developed paralysis or severe leg weakness during the first three weeks of infection. weakness persisted with little subsequent improvement. five to 8 months later, 4 such mice were killed, and each spinal cord was examined in epon sections in a series of transverse sections along their lengths. all cords had 2 or more major white ... | 1983 | 6655486 |
| antiviral and antitumor compounds from tunicates. | tunicates provide a rich source of biologically active compounds with potentially useful medicinal properties. the most interesting compounds identified thus far are the didemnins, depsipeptides from a caribbean trididemnum species, which are potent inhibitors of l1210 leukemia cells in vitro and are also active in vivo against p388 leukemia and b16 melanoma. in addition the didemnins inhibit growth of a variety of rna and dna viruses in vitro and protect mice infected intravaginally with herpes ... | 1983 | 6681537 |
| co-existence of malignant squamous cells and herpes simplex virus type 2-infected cells. | a case is presented in which malignant squamous cells and herpes virus infected cells were recognized concomitantly at routine cytologic examination for detection of cervical cancer. further examinations on admission revealed stage ib carcinoma of the cervix and characteristic pathologic changes of herpetic infection in the tumor cell nests. virus was isolated from the cancerous tissues in the ectocervix following radical hysterectomy, and was identified as herpes simplex virus type 2 (hsv-2). t ... | 1983 | 6681607 |
| [the autoinoculation test. false-positive reactions caused by herpes simplex virus]. | the autoinoculation test has been used as a diagnostic procedure in patients with chancroid-suspected genital ulcerations. in four of 13 patients with a positive autoinoculation test, herpes simplex virus type 2 was isolated from the primary lesions. in two of the cases this virus was also isolated from the inoculation site, and one patient developed a recurrent herpetic eruption here. two patients were proven to have chancroid. three of the remaining seven patients developed recurrent herpes ge ... | 1983 | 6682839 |
| carbocyclic arabinofuranosyladenine (cyclaradine): efficacy against genital herpes in guinea pigs. | carbocyclic arabinofuranosyladenine (cyclaradine), a novel nucleoside analog with such desired features as hydrolytic and enzymatic stability, adenosine deaminase resistance, and low systemic toxicity, inhibited the replication of herpes simplex virus types 1 and 2. the 5'-methoxyacetate prodrug form exhibited significant efficacy in the topical treatment of genital infections by herpes simplex virus type 2. | 1983 | 6684328 |
| vaginal contraception with gossypol: a clinical study. | a clinical study concerning the vaginal contraceptive efficacy of gossypol acetic acid was performed. fifteen women who had undergone tubal sterilization volunteered for the study. the effect of vaginal gossypol-containing gel on spermatozoa was determined by postcoital tests performed in subjects without and after using gossypol gel. after gossypol application, the number of spermatozoa found in cervical mucus was greatly decreased and, in eleven of the fifteen women, all spermatozoa seen were ... | 1983 | 6684533 |
| antiviral activities of cloned human leukocyte interferons against herpes simplex virus type 2 infections of mice. | human alpha-interferons (ifn-alpha s) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (hsv-2) infections of mouse l-cells in vitro, and acute cervicovaginal and lethal systemic hsv-2 infections of balb/c mice. the recombinant dna-derived hybrid interferon ifn-alpha ad(bgl) showed pronounced antiviral activity in vitro, exceeding the activity of either of the parental subtypes ifn-alpha a and ifn-alpha d and that of the other hybrids ifn-alpha ad(pvu) and ... | 1983 | 6684674 |
| lack of efficacy of amp against recrudescent genital herpes infections in guinea pigs. | amp was evaluated for its efficacy against herpes simplex virus type 2 genital infections in guinea pigs. vaginally infected animals were treated twice a day with amp at 50 mg/kg per dose for 2 weeks. subcutaneous doses were started 10 weeks postinfection. amp treatment did not reduce the number of animals with recrudescent episodes when compared with saline-treated controls. in addition, amp treatment did not reduce either the number of lesions or the duration of the recrudescent episodes which ... | 1983 | 6685993 |
| evaluation of prodrugs of 9-beta-d-arabinofuranosyladenine for therapeutic efficacy in the topical treatment of genital herpesvirus infections in guinea pigs. | prodrugs of the antiviral agent 9-beta-d-arabinofuranosyladenine (araa), which were more effective than the parent compound in penetrating vaginal membranes in vitro, were synthesized and examined for efficacy in the topical treatment of genital infections with herpes simplex virus type 2 in female guinea pigs. treatment with 10% araa-5'-monophosphate or 10% araa-5'-monovalerate twice a day for 7 days, starting 6 h after intravaginal inoculation with virus, completely aborted the primary infecti ... | 1983 | 6686429 |
| effect of cp-20,961 on genital herpes in guinea pigs. | cp-20,961 (n,n-dioctadecyl-n',n'-bis(2-hydroxyethyl)propanediamine) has been reported to be an interferon inducer, an adjuvant and a macrophage activator. in the present study, this compound was used therapeutically and prophylactically to treat genital herpes simplex virus type 2 (hsv-2) infections in guinea pigs. a significant decrease in the incidence of clinical lesions (p less than 0.05) was observed in animals treated intravaginally with 20 mg cp-20,961 (two doses each containing 10 mg) pr ... | 1983 | 6686755 |
| neoplastic transformation by a cloned human cytomegalovirus dna fragment uniquely homologous to one of the transforming regions of herpes simplex virus type 2. | specific dna fragments of human cytomegalovirus strain towne exhibited sequence homology to the transforming regions of herpes simplex virus type 2 (hsv-2) when examined by nitrocellulose filter hybridization under nonstringent conditions. cloned towne xba i fragments b and c were homologous to both bgl ii transforming fragments n and c of hsv-2 dna, whereas cloned towne xba i fragment e was uniquely homologous to hsv-2 bgl ii fragment c. furthermore, towne xba i fragment e exhibited homology to ... | 1983 | 6304741 |
| insertion mutants of herpes simplex virus have a duplication of the glycoprotein d gene and express two different forms of glycoprotein d. | we produced insertion mutants of herpes simplex virus (hsv) that contain two functional copies of genes encoding different forms of glycoprotein d (gd). these viruses have the gene for hsv type 2 (hsv-2) gd at the normal locus and the gene for hsv-1 gd inserted into the thymidine kinase locus. results of immunoprecipitation experiments done with monoclonal antibodies revealed that both gd genes were expressed by these viruses, regardless of orientation of the inserted hsv-1 gd gene, and that max ... | 1983 | 6312097 |
| 12-o-tetradecanoyl-phorbol-13-acetate enhancement of the tumorigenic potential of herpes simplex virus type 2 transformed cells. | the consequences of tumor promoter 12-o-tetradecanoyl-phorbol-13-acetate (tpa) interaction with clonal hsv-2-transformed nontumorigenic and weakly tumorigenic cells were studied. enhancement of anchorage-independent growth (cloning efficiency) occurred in a dose-dependent manner when transformed but not nontransformed cells were pretreated with tpa. all subclonal nontumorigenic cell lines progressed to become tumorigenic after 20 serial passages in the presence of tpa. also, subclonal, weakly tu ... | 1983 | 6312388 |
| identification of dna polymerase(s) involved in the repair of viral and cellular dna in herpes simplex virus type 2-infected cells. | when human embryonic fibroblasts (hef) were infected with herpes simplex virus type 2 (hsv-2), replicative viral dna synthesis and some repair synthesis of cellular dna were induced at the early stage of infection, but almost all dna synthesis at the late stage of infection was derived from repair synthesis of cellular and viral dna (y. nishiyama and f. rapp, virology 110, 466-475, 1981). in this study, we have assessed the effects of dna polymerase inhibitors on repair dna synthesis hsv-2-infec ... | 1983 | 6312689 |
| interferon susceptibility of herpes simplex virus strains isolated from patients enrolled in clinical trials. | herpes simplex virus type 1 strains isolated from patients who had received interferon in a clinical trial were not more resistant to human leukocyte interferon than strains derived from recipients of a placebo. the susceptibility of herpes simplex virus type 2 strains isolated from herpes genitalis was slightly less than that of herpes simplex virus type 1 strains causing herpes genitalis. | 1983 | 6312872 |
| antiherpetic activity and mechanism of action of 9-(4-hydroxybutyl)guanine. | 9-(4-hydroxybutyl)guanine was synthesized and tested for antiherpes activity. in cell cultures, different strains of herpes simplex virus type 1 (hsv-1) and type 2(hsv-2) were inhibited by 50% at 2-14 microm of 9-(4-hydroxybutyl)guanine, while a hsv-1 mutant lacking thymidine kinase (hsv-1 tk-) was resistant. linear competitive inhibition of purified hsv-1-induced thymidine kinase (tk) with thymidine as a variable substrate was observed for 9-(4-hydroxybutyl)guanine with an apparent ki value of ... | 1983 | 6312878 |
| role of the nuclear matrix in the growth of herpes simplex virus type 2. | nuclear matrix was prepared from vero cells infected with herpes simplex virus type 2. in the early stage of infection, both 155k and 110k viral proteins were associated with the nuclear matrix, while in the late stage, 155k protein, presumably a viral capsid protein, was predominantly associated with the matrix. electron microscopic study showed that empty capsids were bound to the filamentous networks of the nuclear matrix of the late stage. neither viral dna nor viral dna polymerase activity ... | 1983 | 6312935 |
| replication of herpes simplex virus in two cell systems derived from rhesus monkeys. | the replication of herpes simplex virus (hsv) in two cell systems derived from rhesus monkeys (llc-mk2 and dbs-frhl-2) was studied. in llc-mk2, the growth of hsv-1 was abortive or extremely limited regardless of the multiplicity of infection, while that of hsv-2 was productive only on infection at high multiplicities. dbs-frhl-2 cells supported growth of both types of hsv, although growth was highly dependent on the age of monolayers and the infectious dose of virus inocula. plaques were produce ... | 1983 | 6312959 |
| detection of herpes simplex virus type-2 dna restriction fragments in human cervical carcinoma tissue. | dna extracted from eight human cervical carcinomas, one lymph node metastasis and related control tissue was examined for the presence of herpes simplex virus (hsv) dna sequences. southern blot transfers of tumour and control dna were hybridised with radioactively labelled cloned probes representing 70% of the hsv-2 genome. specific hybridisation to hsv dna sequences was observed in one of eight carcinoma tissues analysed. hybridisation of hsv-2 dna probes to bamhi and xhoi restriction enzyme fr ... | 1983 | 6313349 |
| high-resolution characterization of herpes simplex virus type 1 transcripts encoding alkaline exonuclease and a 50,000-dalton protein tentatively identified as a capsid protein. | four partially overlapping mrnas (1.9, 2.3, 3.9, and 4.5 kilobases [kb]) were located between 0.16 and 0.19 map units on the herpes simplex virus type 1 genome. their direction of transcription was found to be from right to left. the 2.3-kb mrna was found to be early (beta), whereas the others were late (beta gamma). partial sequence analysis of the dna encoding these genes indicated that the promoter for the 2.3-kb mrna shares structural features with other early (beta) promoters. in vitro tran ... | 1983 | 6313961 |
| herpesvirus rna in human urogenital tumors. | in situ hybridization was used to examine human urogenital cancers for herpes simplex virus type 2 (hsv-2) and human cytomegalovirus rnas. neither of these herpes rnas was found in prostate tissues. however, hsv-2 rna was detected in premalignant and malignant cervical tissues. | 1983 | 6314345 |
| [mechanism of the cellular transformation of newborn hamsters inoculated with herpes simplex type 2 virus]. | combined virological, antigenic, electron microscopic, and molecular biologic study of the role of an oncovirus, herpes simplex type 2 virus (hsv-2), in the mechanisms of transformation of hsv-2-infected hamster cells was carried out. no expression of genetic information of the oncovirus could be detected. cell transformation was shown to be associated with persistence and realization of hsv-2 genetic information in the transformed cells. | 1983 | 6314675 |
| dna sequence homology between two co-linear loci on the hsv genome which have different transforming abilities. | a transcription unit at the herpes simplex virus (hsv) type 2 transforming region, mtr-2 (map coordinates 0.580-0.625), comprises two early, unspliced mrnas of 4.5 kb and 1.2 kb which are 3' co-terminal; a region including that specifying the 1.2-kb mrna has been sequenced. the putative translated portions of these two mrnas do not overlap and this feature, together with the arrangement of the mrnas, is similar to the apparently equivalent co-linear hsv-1 locus which however does not transform. ... | 1983 | 6315408 |
| neonatal herpes simplex virus infection in king county, washington. increasing incidence and epidemiologic correlates. | the incidence of neonatal herpes simplex virus (hsv) infection in king county, washington, has increased progressively from a rate of 2.6 per 100,000 live births to king county residents during the years 1966 through 1969 to 4.8, 7.4, and 11.9 cases per 100,000 live births for the years 1970 through 1973, 1974 through 1977, and 1978 through 1981, respectively. detailed interviews of the parents of newborns with neonatal hsv-2 infection revealed clinically symptomatic genital herpes in the mother ... | 1983 | 6315977 |