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neutralization of herpes simplex virus by antibody in amniotic fluid.the ability of amniotic fluid to neutralize herpes simplex virus type 2 (hsv-2) was quantitated and compared with the serum neutralization titer in 158 pregnant women. neutralizing activity was expressed as the ability of 0.1 ml of amniotic fluid to reduce the expected number of plaque-forming units (pfu) in the inoculum by 99%. all amniotic fluid samples from 32 women with serum titers of 1:40 or greater neutralized 5 pfu or more; at term, 96% of these fluid samples neutralized 50 pfu or more, ...19826289206
[humoral immunity to herpes simplex virus type 2 in cervical cancer].the analysis of the results of serological studies revealed differences between the number of positively reacting sera from patients with cervical carcinoma and from normal women. the activity of complement-fixing and virus-neutralizing antibodies in the patients' sera and the frequency of their detection were higher than in the sera from normal subjects. these results in combination with other methods of laboratory examinations may be used for early detection of cervical carcinoma as well as fo ...19826289530
the effect of prostaglandins on the multiplication and cell-to-cell spread of herpes simplex virus type 2 in vitro.herpes simplex virus type 2 (hsv-2) after infecting an individual has the ability to remain latent in nervous tissues. the factors that control herpesvirus infection, latency, and reactivation are poorly understood. fever, menstruation, emotional stress, exposure to sunlight, and surgical resection have been associated with activation of latent herpes. the situations which activate latent herpes are associated with a local or systemic rise in prostaglandins. the data presented show that prostagl ...19826289665
transfection with the isolated herpes simplex virus thymidine kinase genes. ii. evidence for amplification of viral and adjacent cellular dna sequences.we have previously described several series of biochemical transformation experiments in which small defined portions of herpes simplex virus (hsv-1 and hsv-2) dna encompassing the thymidine kinase (tk) gene were introduced into ltk- cells by the calcium transfection procedure. the presence of authentic virus tk enzyme in several subcloned cell lines derived from these experiments was confirmed by either the specific incorporation of [125i]iododeoxycytidine into their nuclei or the inhibition of ...19826286848
genital herpes in guinea pigs: pathogenesis of the primary infection and description of recurrent disease.guinea pigs inoculated intravaginally with herpes simplex virus type 2 (hsv-2) developed a self-limiting infection characterized by vesiculo-ulcerative lesions on the external genital skin, urinary retention, and hindlimb paralysis. infection rarely resulted in death. virologic, histologic, and immunoperoxidase data suggested the following scheme for viral pathogenesis: initial replication in the introitus, vagina, and bladder; spread via sensory nerves to the lumbosacral dorsal root ganglia and ...19826286797
identification and typing of herpes simplex viruses with monoclonal antibodies.monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral dna and by indirect immunofluorescence with monoclonal antibodies. examination of a limited number ...19826286719
genital herpes simplex infections in israel: 1973 throughout 1980.ninety-eight cases of genital herpes simplex virus infection (hsv-2) were confirmed by laboratory examinations during the years 1973-80. until 1975 there were less than five cases per year, but from 1976 the number of cases gradually increased, reaching 24 in 1980. the age distribution is typical of a sexually transmitted disease; and since the peak of susceptibility is at childbearing age, an increase in neonatal hsv infection could be expected. in 1980, laboratory testing confirmed neonatal hs ...19826292133
failure of 2-deoxy-d-glucose in the treatment of experimental cutaneous and genital infections due to herpes simplex virus.the effectiveness of 2-deoxy-d-glucose (2-dg) was evaluated in the treatment of cutaneous infections with herpes simplex virus type 1 (hsv-1) in mice and genital infections with hsv type 2 (hsv-2) in mice and guinea pigs. groups of mice were inoculated in the lumbosacral or orofacial area with hsv-1 and treated topically three times a day with 0.2% or 0.5% 2-dg solution beginning 3 hr after inoculation. no effect on skin lesions, mortality, or latency was observed. mice were inoculated intravagi ...19826286785
clinical and virologic studies on female genital herpes.ninety women with genital herpes were studied clinically and virologically. from a clinical standpoint, these patients could be classified into 3 clinical types--acute, recurrent, and provoked. when herpes simplex virus (hsv) strains isolated from these patients and asymptomatic virus secretors were typed, 36 strains were determined to be type 1 (hsv-1) and the remaining 54 to be type 2 (hsv-2). the acute type was caused by primary infection of hsv-1 or hsv-2 as well as by infection of hsv-2 in ...19826289208
transfection with the isolated herpes simplex virus thymidine kinase genes. i. minimal size of the active fragments from hsv-1 and hsv-2.we have defined the minimal size and physical map locations in the genomes of both herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) for dna sequences capable of conferring stable biochemical transformation under thymidine kinase (tk) selection. the experiments involved transfection of ltk- cells with either isolated virus dna fragments or cloned pbr322 plasmids containing the 3.5 kilobase (kb) bamhi-o fragment from hsv-1(mp) or the 5.6 kb sali-g fragment from hsv-2(333). mapping of restricti ...19826292348
isolation and characterisation of two strains of herpesvirus hominis type 1 from fallopian tubes.herpesvirus hominis type 1 (hsv-1) was isolated from fallopian-tube biopsies of two women who were using an intra-uterine contraceptive device; the strains were isolated on different dates and at different hospitals. both the isolates were shown to be hsv-1 by restriction-enzyme analysis, polypeptide profiles and indirect immuno fluorescence; the two isolates could not be distinguished by these tests, but the tests distinguished them from hsv-1 strain 71-15 and hsv-2 strain 333. the viruses were ...19826292427
glycoproteins of herpes simplex virus type 2 as defined by monoclonal antibodies.we used monoclonal antibodies reacting with glycoproteins specified by herpes simplex virus type 2 (hsv-2) to characterize the individual antigens in terms of structure, processing, and kinetics of synthesis in bhk or vero infected cells. our results provided a direct demonstration of the structural identity of the ga and gb proteins of hsv-2 as well as confirmation of the existence of type-specific and type-common domains within the gd molecule. they also show that, with the exception of gc, pr ...19826292496
herpes simplex virus glycoprotein ga/b: evidence that the infected vero cell products comap and arise by proteolysis.we recently reported (pereira et al., proc. natl. acad. sci. u.s.a. 78:5202-5206, 1981) that herpes simplex virus 1 and 2 glycoproteins, previously designated ga and gb, could not be differentiated by a bank of independently derived type-specific and type-common monoclonal antibodies. we also reported that from lysates of infected vero cells, all but one monoclonal antibody precipitated ga/b glycoproteins which had faster electrophoretic mobility than the corresponding infected hep-2 cell glycop ...19826292507
unusual serologic response to two patients to an early antigen of herpes simplex virus type 2.two patients infected with herpes simplex virus type 2 (hsv-2) who were free of recurrences over a 13-14-month period had an unusually high titer (greater than 1:48) of complement-fixing antibody to the early antigen (ag-4) of hsv-2. their response was also unusual in that it was an igg complement-fixing antibody rather than igm, which normally is found in patients with hsv-2 infection. the antibody was also present 13-14 months after the episode of hsv-2 disease, whereas in other patients, the ...19826293106
[transformation and malignant degeneration of newborn hamster cells inoculated with herpes simplex virus type 2]. 19826293203
comparison of the in vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine and its 5'-monophosphate.in vitro and in vivo anti-herpes activities of 1-beta-d-arabinofuranosylthymine 5'-monophosphate (ara-tmp) were compared with those of 1-beta-d-arabinofuranosylthymine (ara-t). on a molar basis ara-tmp was almost as active as ara-t against six strains of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) as monitored by a cytopathogenicity-inhibition and a plaque reduction assay in human embryonic lung fibroblast cells. when tested against experimental encephalitis in mice inoculated intrace ...19826293376
comparison of restriction endonuclease profiles of dna from local herpes simplex virus type 2 (hsv-2) strains.comparisons of restriction endonuclease profiles of dna isolated from nine genital hsv-2 strains isolated from 1973 to 1981 were made using six restriction endonucleases (bgl ii, hind iii, xba 1, eco ri, bam hi and hpa i). the only variation detected in the dna profiles was found with eco ri and bam hi, and in each case all the isolates could be classified into two groups. the difference between the two eco ri groups could be accounted for by the absence of an eco ri cleavage site from the hsv d ...19826293436
chemical interactions with herpes simplex type 2 virus: enhancement of transformation by selected chemical carcinogens and pro-carcinogens.quantitative assays for the morphological transformation of 3t3 swiss mouse cells by herpes simplex type 2 virus (hsv-2) were employed to examine the effect on cell transformation of chemical carcinogens and pro-carcinogens. the carcinogens tested were n-methyl-n'-nitro-n-nitrosoguanidine, quinacrine mustard, n-nitrosomethyl urea, urethane, and benzene. the pro-carcinogens tested were n-nitrosodimethylamine, 3-methylcholanthrene, benzo[a]pyrene, and p-dimethylaminoazobenzene. exposure of the cel ...19826293737
detection by rna blot hybridization of rna sequences homologous to the bglii-n fragment of herpes simplex virus type 2 dna.rna species, extracted at the time of peak synthesis of the alpha, beta, and gamma classes of herpes simplex virus polypeptides from lytically infected vero cells, were examined for homology to the bglii-n fragment (map units 0.58 to 0.63) of herpes simplex virus type 2 dna. by using northern blot analysis, two major and several minor polyadenylated rna species showed homology to the bglii-n fragment at times corresponding to the maximum synthesis of the beta (7 h postinfection) and gamma (12 h ...19826294326
protection against lethal challenge of balb/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2.monoclonal antibodies secreted by six hybridomas and recognizing antigenic sites on glycoproteins gc, gab, gd, ge, and gf of herpes simplex virus type 2 were examined for their ability to protect balb/c mice from lethal infection by the virus. administration of monoclonal antibodies to individual glycoproteins intraperitoneally 3 h before footpad challenge with 10 times the 50% lethal dose of virus protected between 35 and 75% of the mice, except for one of two monoclonal antibodies recognizing ...19826290390
chromosomal organization of the herpes simplex virus type 2 genome. 19826294981
early interactions of herpes simplex virus with mouse peritoneal macrophages.adsorption of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to resident peritoneal macrophages (pm) of 4-week-old swiss albino (sa) and gr/afib mice was studied. a significantly (p less than 0.05) higher hsv-2 adsorption rate was found with pm of sa mice than with pm of gr/afib mice. of added hsv-2 65% bound to the cells of sa mice over a 120-min period versus 15% to pm of gr/afib mice. only 15 to 20% of added hsv-1 bound to pm regardless of the mouse strain. these patterns of adsorptio ...19826290397
genomic location and lack of phosphorylation of the hsv immediate-early polypeptide ie 12.herpes simplex virus type 1 (hsv-1) induces an immediate-early (ie) polypeptide ie 12. an equivalent polypeptide coded by hsv-2 which migrated slightly more slowly on sds-polyacrylamide gels was identified and designated ie 12.3. analysis of the serotype of the ie polypeptide induced by five hsv-1/hsv-2 intertypic recombinants when correlated with their genome structures showed that ie 12/ie 12.3 mapped in the region spanning the trs/us junction. unlike four other ie polypeptides induced by hsv- ...19826290593
viral receptors on isolated murine and human ependymal cells.viruses that infect ependyma cause ependymitis in humans and hydrocephalus in experimental animals. we report that reovirus type 1 (which induces hydrocephalus in mice) binds to the surface of isolated human and murine ciliated ependymal cells. with the use of recombinant viral clones, the binding property was mapped to the type 1 viral hemagglutinin, which also determines in vivo the affinity of reovirus type 1 for ependyma. mumps virus, measles virus, parainfluenza type 3, and herpes simplex v ...19826276976
confirmation of herpes simplex virus type 2 infections in herpes-like genital lesions by a simple complement-fixation test.the presence of complement-fixing antibody to an early herpes simplex virus type 2 (hsv-2) antigen (the ag-4 antigen) was correlated with hsv-2 infection in the sera of patients with genital herpes. eighty-eight per cent of sera taken two weeks after clinical diagnosis of a primary or recurrent herpes infection in patients, confirmed to have hsv-2 by virus isolation and typing, contained the anti-ag-4 complement-fixing antibody. none of the patients with genital hsv-1 had the antibody, and only ...19826275941
optimal treatment of herpes simplex virus encephalitis in mice with oral acyclovir.the effect of oral or intraperitoneal administration of acyclovir was evaluated in four experimental models of herpes simplex virus (hsv) encephalitis in mice. mice were inoculated with hsv-1 or hsv-2 intracerebrally or with hsv-2 intranasally, intraperitoneally, or intravaginally. with all four routes of inoculation, oral acyclovir therapy significantly reduced mortality when started as late as 72 to 96 hours after viral challenge. intraperitoneal acyclovir was not effective in protecting mice ...19827102699
epidemiology of sexually transmitted diseases in the scandinavian countries.in the scandinavian countries, laws require reporting of the traditional venereal diseases, i.e., gonorrhea, syphilis, lymphogranuloma venereum (lgv), and ulcus molle, or chancroid. incidence figures for these diseases, therefore, are fairly reliable. in the scandinavian countries, lgv and chancroid are rare diseases, and the syphilis incidence has remained low. these three diseases are typically male afflictions. gonorrhea outnumbers the other diseases mentioned and at present has a nearly 1:1 ...19826958011
virus-specific antibodies in sera from patients with genital herpes simplex virus infection.virus-specific antibodies against a number of herpes simplex virus type 2 antigens were determined by radioimmunoprecipitation assays in sequential serum samples obtained from 12 patients with initial genital herpes simplex virus infection. the progressive appearance of antibodies to virus-specific antigens was observed; antibodies against a 130,000-molecular-weight glycoprotein complex appeared first, followed by antibodies against the major nucleocapsid polypeptide and then antibodies against ...19827118244
lethal encephalitis and non-lethal multifocal central nervous system demyelination in herpes simplex virus type 2 infections in mice.six-week-old swiss-webster mice were infected intracerebrally with a low dose of herpes simplex virus type 2 (hsv-2) and were studied by pathological, virological and immunological methods. one third of mice developed severe neurological disease and died during the first 31/2 weeks of infection, while the remaining two thirds survived this acute stage with relatively minor neurological signs. these survivors had multifocal demyelinative white-matter lesions in the cns, and gray-matter lesions, i ...19827150512
immunity to herpes simplex virus type 2. iv. impaired lymphokine production during recrudescence correlates with an imbalance in t lymphocyte subsets.cell-mediated immunity (cmi) to herpes simplex virus type 2 (hsv-2) was studied in patients with recurrent disease. peripheral blood lymphocytes (pbl) obtained during recrudescence (0 to 3 days post-onset of lesion), convalescence (4 to 14 days), and quiescence (greater than 14 days), which were cultured in vitro in the presence of uv-inactivated hsv-2 (g) antigen, demonstrated similar, virus-specific lymphoproliferative responses. however, the production of the lymphokine, leukocyte migration-i ...19827045224
spinal cord and optic nerve demyelination in experimental herpes simplex virus type 2 infection.to produce a model of central nervous system (cns) demyelination, three strains of herpes simplex virus type 2 (hsv-2) were injected intracerebrally into swiss-webster outbred mice. animals developed pupillary abnormalities and leg weakness and, by light and electron microscopy, had focal lesions of primary demyelination in optic nerves and spinal cords. oligodendrocytes and astrocytes in these lesions contained herpesvirus. both mouse age and virus dose were important determinants of the rate a ...19827077361
treating viral venereal infections with the co2 laser.viral venereal infections caused by herpes simplex virus 2 and papillomavirus (wart) are epidemic diseases in the united states. unfortunately, these two infections have eluded conventional therapy to date. therefore, 306 women were treated for viral venereal diseases with the co2 laser. two hundred twenty-eight patients were treated for condyloma acuminatum and 78 for genital herpes. all the patients who underwent treatment for herpes were enrolled in an investigative protocol. since the viral ...19826819358
aseptic meningitis and herpes simplex proctitis. a case report.a male homosexual patient developed an aseptic meningitis during the course of acute ano-proctitis due to herpes simplex virus type 2 (hsv-2). aseptic meningitis (sometimes proved to be due to hsv-2) has been associated with genital but not with anal herpes. this sexually transmissible virus should be considered when patients with aseptic meningitis are investigated.19827198922
epidural analgesia in the presence of herpes simplex virus (type 2) infection. 19827201280
congenital herpes simplex virus, type 2, bilateral endophthalmitis.a set of dizygotic twins, both born prematurely, developed herpes simplex encephalitis shortly after birth. the second twin had unilateral keratoconjunctivitis and bilateral endophthalmitis and subsequently died from disseminated herpes infection. herpes simplex, type 2, was isolated from conjunctiva of both eyes, cerebrospinal fluid, nasopharynx and trachea. histologic examination of the eyes revealed bilateral endophthalmitis with a necrotizing retinitis. intranuclear inclusion bodies were dem ...19826985228
herpes simplex type 2 virus encephalitis presenting as psychosis.the current literature recognizes two antigenic types of herpes simplex virus, type 1 and 2. type 1 is the most common cause of sporadic necrotizing encephalitis in the united states, with a mortality rate of 30 to 70 percent, and leaves various neurologic sequelae in the survivors. herpes simplex virus type 2 had been recognized as an etiologic agent in fatal infections in neonates and a mild meningitis in adults, but its role in encephalitis in adults is less well known. we report a case of he ...19827124772
modulation by cyclosporin a of murine natural resistance against herpes simplex virus infection. i. interference with the susceptibility to herpes simplex virus infection.adult balb/c mice which are medium-high resistant against intraperitoneal (i.p.) infection with herpes simplex virus type 2 (hsv-2) manifested a drastic increase in susceptibility to the virus when treated locally with cyclosporin a (cya) during infection. oral application of the drug had no effect on the natural resistance status. mice appeared normal 2 weeks after cya treatment with regard to their ability to resist i.p. infections. cya did not interfere with established specific immune protec ...19826980627
intertypic superinfection of herpes simplex virus in vivo leads to reisolation of latent viruses with newly acquired lymphotropic properties.the susceptibility of juvenile tupaias to several strains of temperature-sensitive mutants of hsv-1 and 2 was tested. it was found that in all cases the animal survived an infection of 1 x 10(7) pfu of temperature-sensitive mutants which were administered intravenously. in contrast, an infection dose of 1 x 10(3) pfu of wild-type hsv-2 and 1 x 10(2) pfu or hsv-1 was fatal for juvenile tupaias. those animals which had initially been infected with the ts-mutants of hsv-1 and/or 2 were protected ag ...19826299861
monoclonal antibodies to three non-glycosylated antigens of herpes simplex virus type 2. 19826296279
properties of a herpes virus-transformed hamster cell line--i. growth and culture characteristics of sublines of high and low metastatic potential.a herpes virus hominis type 2-induced tumour line of the syrian hamster, designated hsv-2-333-2-26, exhibited a low level of spontaneous metastasis from the primary subcutaneous tumour mass. two lung foci, met a and met b, were resected and transplanted s.c.; both lines showed an elevated metastatic potential compared with the parent cell line such that all animals inoculated with these cells developed metastatic disease within 40 days after resection of the primary tumour. neither the rapidity ...19826297914
virus-induced thymidine kinases as markers for typing herpes simplex viruses and for drug sensitivity assays.a rapid, reproducible and objective new method for typing herpes simplex viruses type 1 (hsv-1) and type 2 (hsv-2) based on the effects of virus-induced thymidine kinases on various antiviral drugs has been developed. when several laboratory strains and clinical isolates were typed by this method and compared to the results obtained by the immunofluorescence antibody typing method, agreement was found for all the viruses. the new technique has the added advantage of determining the sensitivity o ...19826296179
modification of herpes 2-transformed cell-induced tumors in mice fed different sources of protein, fat and carbohydrate.the effects of different sources of protein (milk, soy, wheat, fish and beef), fat (corn oil and butter), and carbohydrate (dextrin and sucrose) on tumor development and on spleen characteristics were investigated in balb/c mice injected subcutaneously with 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells). low or high levels of protein and fat were used. several weeks post-injection results indicated that a high level of fat significantly enhanced tumor incidence. a high fat ...19826299511
herpes virus-related antigens in herpes simplex virus type 2-transformed cells in the course of cervical carcinoma.anticomplement immunofluorescence (acif) technique was applied to herpes simplex virus type 2 (hsv-2)-transformed cells (333-8-9) and to cells lytically infected with hsv-2 in sera from women with cervical carcinoma. there was a correlation between the positive results with both types of cells. long-term survivors suffering from cervical cancer showed a high percentage of positive immunofluorescence (if) reactions in hsv-2 transformed cells (77%) as well as in cells lytically infected with hsv-2 ...19826299751
imide derivatives of 3-nitro-1.8-naphthalic acid: their inhibitory activity against dna viruses.the antiviral action of a new drug, 5-amino-2-(dimethylaminoethyl)-benzo-[de]-isoquinolin-1.3-dione has been studied against herpes simplex type 2 (hsv-2) and adenovirus type 5 (ad-5) grown in vero cells. the concentration of the drug which gives a 5 log10 reduction in virus titer was 4 micrograms/ml (maximum tolerated concentration) for hsv-2. the anti-hsv-2 activity of this compound was one log. more powerful than that of iododeoxyuridine (idu). at this concentration the drug shows virucidal a ...19826299235
abortive infection of neural cells by herpes simplex virus type 2.the nature of the refractoriness of c6 rat glioma cells to herpes simplex virus type 2 (hsv-2) was examined. infection of c6 cells with hsv-2 results in low virus yields, not exceeding the input virus. although virus growth studies suggested a restricted cycle of virus replication, synthesis of hsv-2 dna and hsv-2-specific antigens could not be detected. in addition, hsv-2 yields in c6 cells were unaffected by interferon, cycloheximide, tunicamycin, actinomycin d and cytosine arabinoside. howeve ...19826299239
characterization of an herpes simplex virus type 2 mutant, which is resistant to acycloguanosine and causes fusion of bsc1 cells.a mutant of herpes simplex virus type 2, which induces low levels of thymidine-kinase activity in infected bsc1 cells and consequently able to grow in the presence of acycloguanosine, was isolated. this mutant has also been shown to cause fusion of bsc1 cells. in bsc1 cells, co-infected with the wild-type strain and the mutant, the yield of each of the two viruses was normal but the rounding and aggregation of cells observed, resembled that found in wild-type infected cultures. when the mixed in ...19826299232
herpes simplex virus subunit vaccine: characterization of the virus strain used and testing of the vaccine.herpes simplex virus type 1 (hsv-1) used for vaccine production was isolated from a nasal recurrent infection and propagated over a limited number of passages in human diploid cells only. it was designated as hsv-1 bw3 and has been characterized by neutralizing antibodies as a typical hsv type 1 strain. in vitro transformation studies performed with this isolate in mouse or hamster cells revealed only very low, if any, transforming capacity. a preparation of hsv-1 bw3 which can be used as seed l ...19826299841
the role of herpes simplex virus in cervical and vulvar cancer.this report extends the association of herpes simplex virus type 2 (hsv2) with cervical cancer to vulvar carcinoma. in addition to seroepidemiologic observations linking prior hsv2 infection to genital cancer, the new evidence involves the neoplasms themselves. biopsies of cervical and vulvar cancers were found to contain hsv2-specified antigens. the virus-induced antigens are nonstructural dna-binding proteins normally present in the nuclei of infected cells; however, in the cancer cells they w ...19826299862
synergism among biolf-62, phosphonoformate, and other antiherpetic compounds.9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]guanine (biolf-62) is highly synergistic with either phosphonoformate or phosphonoacetate when used in combination against herpes simplex virus types 1 and 2 in vitro. acycloguanosine did not show significant synergism with these two compounds. bromovinyldeoxyuridine and phosphonoformate were highly synergistic against herpes simplex virus type 2, but not against type 1.19826297382
different weekly changes in immune response in virus-transformed cell-injected mice fed two different diets.changes in tumor development and in certain immune responses were investigated at 7-weekly intervals after subcutaneous injection of 5 x 10(5) herpes simplex virus type 2-transformed cells (h238 cells) into male balb/c mice fed 2 different diets. one diet contained 11% casein and 5% fat while the other had 11% supplemented wheat gluten and 30% fat. weanling mice (140/group) were fed one or the other of the diets for 12 weeks before injection and subsequent testing of 15 injected and 5 non-inject ...19826299512
inapparent genital herpes simplex virus infection in college women.during a six-month period, 600 gynecological samples were collected from 585 women with typical herpes lesions, women with non-herpes symptoms (ie, vaginitis, moniliasis, trichomoniasis, etc), and normal women seen at the student health center gynecological clinic and processed for herpes simplex virus (hsv) isolation. from these specimens, 29 samples from 25 of the 585 women (4.3%) were positive for hsv. when these isolates were typed using plaque diameter in chick cells, heat stability of vira ...19826298359
use of monoclonal antibodies to hsv-1 and hsv-2 for serological analysis of the viral glycoproteins.herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) specify four major glycoproteins designated ga/gb, gc, gd and ge. type-specific and cross-reacting monoclonal antibodies to the viral glycoproteins have been used to probe their antigenic determinants. our studies show the following: (i) type-specific monoclonal antibodies are suitable reagents for serotyping isolates. discriminating monoclonal antibodies distinguish antigenic variants among the strains of each serotype. (ii) glycoproteins ga ...19826299824
development of sensitive and enzyme-linked immunoassays for herpesvirus antigens and some applications.sensitive enzyme-linked immunoassays for herpes simplex virus (hsv), cytomegalovirus (cmv) and varicella-zoster virus (vzv) were developed. both a sandwich technique, using antiviral antibodies from two species to detect the antigen, and an inhibition assay where the sample antigen was incubated with one antiserum, could be used. around 4-50 ng of viral antigens (measured as protein content) could be detected. the elisa inhibition technique using type-specific antisera could differentiate betwee ...19826299833
application of molecular genetics to the design of live herpes simplex virus vaccines.in principle, several avenues for attenuation of herpes simplex viruses (hsv) are now available. these include intermixing of hsv-1 and hsv-2 genes by recombination, altering the regulation of gene expression and the deletion of viral genes not required for replication of the virus in permissive cells in culture. results of analyses of hsv-1 x hsv-2 recombinants and of mutants containing a deletion in a gene expressed early in infection showed a loss of virulence when infected by intracerebral r ...19826299836
influenza type a virus-mediated adherence of type 1a group b streptococci to mouse tracheal tissue in vivo.influenza type a virus-mediated adherence of pathogenic bacteria to the mucosal surface of the respiratory tract may be one of several mechanisms whereby influenza predisposes to bacterial pneumonia. in the present study, we quantified the adherence of intranasally administered type 1a group b streptococci to the tracheal tissue of influenza type a/pr8/34 (honi) virus-infected and mock-infected mice. influenza type a/pr8/34 virus infection effected a 120-fold increase in the adherence of type 1a ...19826754623
vaginal contraception with gossypol: a clinical study.a clinical study concerning the vaginal contraceptive efficacy of gossypol acetic acid was performed. fifteen women who had undergone tubal sterilization volunteered for the study. the effect of vaginal gossypol-containing gel on spermatozoa was determined by postcoital tests performed in subjects without and after using gossypol gel. after gossypol application, the number of spermatozoa found in cervical mucus was greatly decreased and, in eleven of the fifteen women, all spermatozoa seen were ...19836684533
antiviral activities of cloned human leukocyte interferons against herpes simplex virus type 2 infections of mice.human alpha-interferons (ifn-alpha s) made in bacteria were examined for antiviral activity against herpes simplex virus type 2 (hsv-2) infections of mouse l-cells in vitro, and acute cervicovaginal and lethal systemic hsv-2 infections of balb/c mice. the recombinant dna-derived hybrid interferon ifn-alpha ad(bgl) showed pronounced antiviral activity in vitro, exceeding the activity of either of the parental subtypes ifn-alpha a and ifn-alpha d and that of the other hybrids ifn-alpha ad(pvu) and ...19836684674
herpetic whitlow: report of a case with multiple recurrences.a case of recurrent herpetic whitlow is discussed. the patient, a dental assistant at the time of her initial infection, developed herpetic whitlow caused by herpes simplex virus type 2 (hsv-2). she has subsequently experienced nine recurrent infections with lesion spread, scarring, and persistent paresthesia. the severity of the most recent recurrences appears to have diminished, and prodromal sensations, that is, increased local sensitivity, have occurred without the subsequent development of ...19836576289
therapeutic activities of 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and -thymine alone and in combination with acyclovir and vidarabine in mice infected intracerebrally with herpes simplex virus.the therapeutic effectiveness of two new antiviral agents, 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)-5-iodocytosine and 1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)thymine, was compared with that of acyclovir and vidarabine. in mice inoculated intracerebrally with high 50% lethal doses of herpes simplex virus type 2, nontoxic intraperitoneal or oral treatments with the two new fluorinated antiviral agents were highly effective in reducing mortality. the two drugs were also effective when trea ...19836625559
lack of efficacy of amp against recrudescent genital herpes infections in guinea pigs.amp was evaluated for its efficacy against herpes simplex virus type 2 genital infections in guinea pigs. vaginally infected animals were treated twice a day with amp at 50 mg/kg per dose for 2 weeks. subcutaneous doses were started 10 weeks postinfection. amp treatment did not reduce the number of animals with recrudescent episodes when compared with saline-treated controls. in addition, amp treatment did not reduce either the number of lesions or the duration of the recrudescent episodes which ...19836685993
carbocyclic arabinofuranosyladenine (cyclaradine): efficacy against genital herpes in guinea pigs.carbocyclic arabinofuranosyladenine (cyclaradine), a novel nucleoside analog with such desired features as hydrolytic and enzymatic stability, adenosine deaminase resistance, and low systemic toxicity, inhibited the replication of herpes simplex virus types 1 and 2. the 5'-methoxyacetate prodrug form exhibited significant efficacy in the topical treatment of genital infections by herpes simplex virus type 2.19836684328
increase in laboratory-confirmed cases of herpes genitalis and neonatal herpes infections in israel.jewish women have been considered to be at low risk for genital herpes simplex virus type 2 (hsv-2) infections. during the period 1973 to 1981, genital herpes simplex infections were laboratory confirmed in 129 cases (81 women and 48 men). until 1976, only sporadic cases were reported to our laboratory. since then, the number of cases has gradually increased, reaching 31 new reported cases in 1981. the age distribution was typical for a sexually transmitted disease, with the peak of infection at ...19836662679
congenital absence of scalp skin and herpes simplex virus. a case report.a male infant, born at 40 weeks' gestation of a 27-year-old mother, presented at delivery with three scalp lesions, 2 to 4 cm in diam, covered with necrotic tissue and devoid of hair. the infant was born by natural vertex delivery within 2 hours of membrane rupture; birth weight was 3,300 g and apgar score 10. there were no other skin lesions, and physical and neurological examinations were normal. herpes simplex virus type 2 was isolated from the lesions and the mother's cervix. high titers of ...19836662680
[the autoinoculation test. false-positive reactions caused by herpes simplex virus].the autoinoculation test has been used as a diagnostic procedure in patients with chancroid-suspected genital ulcerations. in four of 13 patients with a positive autoinoculation test, herpes simplex virus type 2 was isolated from the primary lesions. in two of the cases this virus was also isolated from the inoculation site, and one patient developed a recurrent herpetic eruption here. two patients were proven to have chancroid. three of the remaining seven patients developed recurrent herpes ge ...19836682839
evaluation of prodrugs of 9-beta-d-arabinofuranosyladenine for therapeutic efficacy in the topical treatment of genital herpesvirus infections in guinea pigs.prodrugs of the antiviral agent 9-beta-d-arabinofuranosyladenine (araa), which were more effective than the parent compound in penetrating vaginal membranes in vitro, were synthesized and examined for efficacy in the topical treatment of genital infections with herpes simplex virus type 2 in female guinea pigs. treatment with 10% araa-5'-monophosphate or 10% araa-5'-monovalerate twice a day for 7 days, starting 6 h after intravaginal inoculation with virus, completely aborted the primary infecti ...19836686429
effect of cp-20,961 on genital herpes in guinea pigs.cp-20,961 (n,n-dioctadecyl-n',n'-bis(2-hydroxyethyl)propanediamine) has been reported to be an interferon inducer, an adjuvant and a macrophage activator. in the present study, this compound was used therapeutically and prophylactically to treat genital herpes simplex virus type 2 (hsv-2) infections in guinea pigs. a significant decrease in the incidence of clinical lesions (p less than 0.05) was observed in animals treated intravaginally with 20 mg cp-20,961 (two doses each containing 10 mg) pr ...19836686755
topography of remyelinated chronic spinal cord lesions in herpes simplex virus type 2 infections of mice.six-week-old outbred mice were infected intracerebrally with a low dose of the ms strain of herpes simplex virus type 2. about 1% of neurologically abnormal survivors developed paralysis or severe leg weakness during the first three weeks of infection. weakness persisted with little subsequent improvement. five to 8 months later, 4 such mice were killed, and each spinal cord was examined in epon sections in a series of transverse sections along their lengths. all cords had 2 or more major white ...19836655486
efficacy of the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine against primary and recrudescent genital herpes simplex virus type 2 infections in guinea pigs.the acyclic nucleoside 9-(1,3-dihydroxy-2-propoxymethyl)guanine (dhpg) was evaluated for its efficacy in protecting guinea pigs from primary and recrudescent infections of herpes simplex virus type 2. vaginally infected guinea pigs were treated twice a day with dhpg at 25 mg/kg per dose for 3 weeks. subcutaneous doses were started 3 h, 24 h, or 5 weeks after virus inoculation. treatment starting at 3 or 24 h reduced the severity of the primary infection by greater than 70% when lesions were grad ...19836607031
effect of prior immunization on induction of cervial cancer in mice by herpes simplex virus type 2.previous studies at this laboratory showed that repeated application of inactivated herpes simplex virus type 2 to the mouse cevix produced premalignant and malignant lesions. in the present study mice were inoculated with inactivated herpes simplex virus type 2 or control solution and freund's adjuvant by intraperitoneal and subcuaneous routes before exposure of the cervix to inactivated virus. it appears that immunization with inactivated virus conferred a protection against the induction of c ...19836316503
rapid diagnosis of varicella-zoster virus infection by detection of viral deoxythymidine kinase in serum and vesicle fluid.a sensitive enzyme assay utilizing [125i]iododeoxyuridine as the substrate and ctp as the phosphate donor in combination with isozyme-specific antisera was used for direct detection and typing of herpesvirus deoxythymidine kinase (dtk) in clinical specimens. an investigation of 16 coded vesicle fluid specimens, taken in connection with varicella-zoster virus (vzv) and herpes simplex virus infections, revealed viral dtk activity in 14 samples. all positive samples except one were taken within 5 d ...19836339548
current status and prospects for oral acyclovir treatment of first episode and recurrent genital herpes simplex virus.herpes simplex genitalis is an increasingly common primary or recurrent hsv infection. acyclovir (acv) has been found to diminish virus shedding and clinical symptoms in patients with first episode genital hsv when given topically or intravenously. as most patients with first episode disease do not require hospitalization and recurrent disease is usually less severe than the primary infection, out-patient treatment forms the basis of practical management. acyclovir is absorbed orally with plasma ...19836355052
preferential inhibition of 5-trifluoromethyl-2'-deoxyuridine phosphorylation by 5'-amino-5'-deoxythymidine in uninfected versus herpes simplex virus-infected cells.the cytotoxic effects of 5-trifluoromethyl-2'-deoxyuridine (cf3durd) were effectively antagonized by 5'-amino-5'-deoxythymidine (5'-adthd). the antiproliferative actions of cf3durd were reduced in a dose-dependent manner by 5'-adthd in both hela and vero cells. in addition, the ability of cf3durd to kill hela cells (95% at 1 microm and 99% at 3 microm), as measured by cloning efficiency, was ablated entirely by 5'-adthd (300 microm). in contrast, the inhibition of herpes simplex virus type 2 (hs ...19836408395
lysis of herpesvirus-infected cells by macrophages activated with free or liposome-encapsulated lymphokine produced by a murine t cell hybridoma.thioglycolate-induced mouse peritoneal macrophages were activated in vitro by the lymphokine designated macrophage-activating factor (maf) produced by a murine t cell hybridoma to lyse herpes simplex virus type 2 (hsv-2)-infected murine target cells. comparison of uninfected balb/c 10e2 cells with hsv-2-infected 10e2 cells showed that macrophages activated with maf selectively destroyed hsv-2-infected cells and left uninfected cells unharmed, as measured by an 18-h 51cr-release assay. in contras ...19836358037
hsv 2-associated antigens in human cervical tissues before and after grafting in nude mice.biopsy material from human invasive cervical carcinoma, carcinoma in situ (cis) and normal uterine cervix was transplanted into nude mice, some of which were then injected with estrogen or progesterone during a follow-up of 1-4 weeks. the cervical cells were analysed prior to and after passage in nude mice by the anticomplement immunofluorescence (acif) assay for the presence of herpes virus associated antigens. such antigens were found in a few cells in three out of seven transplanted cervical ...19836320579
poly(adp-ribosyl)ation of a herpes simplex virus immediate early polypeptide.this paper reports in vitro poly(adp-ribosyl)ation of the herpes simplex virus type 1 (hsv-1) immediate early polypeptide vmw175. the phenomenon was most clearly observed by use of the temperature-sensitive mutant tsk, which overproduces vmw175 at the nonpermissive temperature (npt) and has a mutation in the coding sequences for this polypeptide. nuclei prepared from cells which were infected with tsk at npt and subsequently downshifted to the permissive temperature incorporated [32p]nad into vm ...19836318441
serotyping of herpes simplex virus isolates: a comparison of bvdu sensitivities, indirect immunofluorescence with monoclonal antibodies, and indirect immunofluorescence with cross-adsorbed rabbit antibodies.four methods for typing of herpes simplex virus (hsv) isolates were compared for 43 recent clinical isolates and 3 reference strains of hsv. these isolates were subjected to indirect immunofluorescence using both monoclonal antibodies and cross-adsorbed rabbit antisera. sensitivity to e-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was also examined. all isolates which fluoresced with hsv-1 monoclonals were found to be sensitive to bvdu with id50's ranging from 0.001 to 0.006 micrograms/ml. all isolat ...19836317706
expression and cellular compartmentalization of a herpes simplex virus type 2 protein (icp 10) in productively infected and cervical tumor cells.antiserum to icp 10, a herpes simplex virus type 2 (hsv-2) protein that is expressed in cells neoplastically transformed by viral dna sequences within the bgl ii/hpa i cd fragment, specifically precipitates the icp 10 protein from hsv-2 infected cells and stains cells infected with hsv-2 for 4 to 16 hrs by indirect immunofluorescence. at 4 hr post infection (p.i.), the staining is primarily perinuclear, while at 16 hr p.i., it is cytoplasmic and intranuclear. compartmentalization studies indicat ...19836320993
[scanning microscopy of hamster cells transformed by herpes simplex virus type 2].scanning microscopy was used to examine the features of morphology and attachment to a solid substrate of a transformed and tumor lines of hamster cells. these cell lines differed from normal hamster fibroblasts by changes in the mode of attachment and the degree of flattening on the solid substrate, relief of the cell surface and pattern of intercellular interactions. the observed morphological changes correlated with the degree of cell transformation.19836318450
cell surface alterations induced by the acycloguanosine (acg) resistant and sensitive strains of herpes simplex virus type 2 (hsv-2) in the presence of acg and tunicamycin.hsv-2-acg, a mutant of herpes simplex virus type 2, which is resistant to acycloguanosine and also capable of inducing fusion of bsc1 cells, was isolated. the viral-induced surface changes in these cells were studied using scanning electron microscopy. the formation of gigantic fused cells with increased number of microvilli and virus-like particles, extensively covering the infected cell surface, were investigated. tunicamycin (50 micrograms/ml), an inhibitor of glycosylation of polypeptides, b ...19836322282
[evaluation of 80 virologically-confirmed cases of ocular herpes. study of the correlation between the type of virus and the epidemiologic characteristics of the disease].80 strains were isolated from patients with herpetic ocular infection during the period may 1979 to may 1981: 17 patients with herpetic blepharitis, 40 patients with superficial keratitis and 23 with deep stromal keratitis or kerato-uveitis. among 63 patients treated with antiviral agents, clinical resistance to the drug was observed in 17 cases. the strains were typed as hsv 1.1, 1.2 or 2.2 by seroneutralization and thermosensitivity in cellular cultures. no relationship between the viral types ...19836323567
antiviral effects of hygromycin b, a translation inhibitor nonpermeant to uninfected cells.hygromycin b, a preferential translation inhibitor in virus-infected cells, has been tested for its antiviral effects against herpes simplex virus type 2 and poliovirus. the activity has been compared with other antiviral agents such as vidarabine and iododeoxyuridine.19836314888
dna sequence of the herpes simplex virus type 2 glycoprotein d gene.we describe a 1635-bp herpes simplex virus type 2 (hsv-2) dna sequence containing the entire coding region of glycoprotein d (gd-2). the amino acid sequence of gd-2, deduced from the nucleotide sequence, was compared to that of the analogous herpes simplex virus type 1 (hsv-1) glycoprotein (gd-1). the two glycoproteins are 85% homologous and contain highly conserved regions of as much as 49 amino acids in length. comparison of dna sequences upstream from gd-1 and gd-2 coding regions identified p ...19836323270
molecular genetics of herpes simplex virus. viii. further characterization of a temperature-sensitive mutant defective in release of viral dna and in other stages of the viral reproductive cycle.previous studies have shown that cells infected with the herpes simplex virus 1(hfem) mutant tsb7 and maintained at the nonpermissive temperature fail to accumulate viral polypeptides. analyses of intertypic recombinants generated by marker rescue of tsb7 with herpes simplex virus 2 dna fragments localized the mutation between 0.46 and 0.52 map units on the viral genome (knipe et al., j. virol. 38:539-547, 1981). in this paper we report that the mutation in tsb7 affects several aspects of the re ...19836296445
herpes simplex virus non-structural proteins. iii. function of the major dna-binding protein.the herpes simplex virus type 2 major dna-binding protein has been functionally characterized using temperature-sensitive mutants in the complementation group 2-2. the mutants were shown to be defective in the dna-binding protein gene by mapping the mutants to the area of the genome known to code for the protein, and by demonstrating alterations in the major dna-binding protein induced in mutant-infected cells. the mutants were shown to be defective in the replication of virus dna. the nature of ...19836302216
neoplastic transformation by a cloned human cytomegalovirus dna fragment uniquely homologous to one of the transforming regions of herpes simplex virus type 2.specific dna fragments of human cytomegalovirus strain towne exhibited sequence homology to the transforming regions of herpes simplex virus type 2 (hsv-2) when examined by nitrocellulose filter hybridization under nonstringent conditions. cloned towne xba i fragments b and c were homologous to both bgl ii transforming fragments n and c of hsv-2 dna, whereas cloned towne xba i fragment e was uniquely homologous to hsv-2 bgl ii fragment c. furthermore, towne xba i fragment e exhibited homology to ...19836304741
biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.a biotin-avidin-amplified enzyme-linked immunosorbent assay (b-a elisa) has been developed to detect herpes simplex virus type 1 (hsv-1) and hsv-2 antigens in clinical specimens. the test was designed as a solid-phase, double-antibody, sandwich assay in which plates were coated with a polyclonal rabbit immunoglobulin g anti-hsv reagent, and the sandwich antibody was a biotin-labeled mouse immunoglobulin m monoclonal antibody that reacts with a common antigen associated with hsv-1 and hsv-2. the ...19836317711
acyclovir treatment of experimental genital herpes simplex virus infections. i. topical therapy of type 2 and type 1 infections of mice.intravaginal inoculation of mice with herpes simplex virus (hsv) provides a model infection of genital herpes to determine the effectiveness of potential antiviral agents. topical (intravaginal) treatment with 1% or 5% acyclovir (acv) in an ointment of gel vehicle initiated 3, 6 or 24 h after inoculation with hsv type 2, significantly inhibited viral replication in the genital tract and usually reduced final mortality. treatment with 5% acv initiated 48 or 72 h after infection also reduced vagin ...19836320718
sequential genital infections by herpes simplex viruses types 1 and 2: restriction nuclease analyses of viruses from recurrent infections.virus isolated from a woman presenting with the first symptomatic episode of genital herpes was identified as herpes simplex virus type 1 (hsv-1) by restriction nuclease fingerprinting. testing for igm antibody to hsv indicated that the patient had recently contracted a new hsv infection. virus microneutralization and the micro-solid phase radioimmunometric test for igg, however, showed that the patient had had prior infection with herpes simplex virus type 2 (hsv-2); thus the hsv-1 infection wa ...19836318371
[level of cyclic nucleotides (camp and cgmp), atpase activity and calcium ion transport in hamster lymphocytes during tumor growth].the biochemical systems of lymphocytes of the peripheral blood and lymph nodes of hamsters with a herpes simplex (type 2) virus--induced transplantable tumor rapp hsv2 were studied. the camp concentration in tumor-bearing hamsters was 1.5-2 times higher, while cgmp level--2.2 times lower than in control. this was matched by a rise in the level of transport atpases in the lymphocytes of experimental animals. concanavalin a had practically no effect on the uptake of ca2+ by lymphocytes of tumor-be ...19836301159
replication of non-respiratory viruses in tracheal organ cultures.employing hoorn's technique, tracheal explant cultures were set from adult hamsters, rabbits and human foetuses. to determine the susceptibility of these cultures they were infected with nine different mainly non-respiratory viruses. assay of virus was carried out in susceptible cell lines. the results of these studies indicated that herpes simplex type i (hsv-1) and vaccinia viruses multiplied in these cultures and caused ciliostasis. herpes simplex type 2 (hsv-2) although multiplying in all, p ...19836301520
use of monoclonal antibodies against two 75,000-molecular-weight glycoproteins specified by herpes simplex virus type 2 in glycoprotein identification and gene mapping.we produced two monoclonal antibodies that precipitate different glycoproteins of similar apparent molecular weight (70,000 to 80,000) from extracts of cells infected with herpes simplex virus type 2. evidence is presented that one of these glycoproteins is the previously characterized glycoprotein ge, whereas the other maps to a region of the herpes simplex virus type 2 genome collinear with the region in herpes simplex virus type 1 dna that encodes gc.19836300459
herpes simplex virus proctitis in homosexual men. clinical, sigmoidoscopic, and histopathological features.acute herpes simplex virus (hsv) infection was detected in 23 of 102 consecutively examined, sexually active male homosexuals who presented with anorectal pain, discharge, tenesmus, or hematochezia, as compared with 3 of 75 homosexual men without gastrointestinal symptoms (p less than 0.01). findings that were significantly more frequent in men with hsv proctitis than in men with proctitis due to other infectious causes included fever (48 per cent), difficulty in urinating (48 per cent), sacral ...19836300674
application of denatured, electrophoretically separated, and immobilized lysates of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins.we report the use of herpes simplex virus type 1 (hsv-1)- and hsv-2-infected cell polypeptides (icps) separated by electrophoresis in polyacrylamide gels and transferred to nitrocellulose to (i) detect monoclonal antibodies to viral polypeptides and to (ii) study the properties of the proteins with the monoclonal antibodies. our results were as follows. (i) when the antigens were electrophoretically separated in denaturing gels and then immobilized on nitrocellulose strips, we detected a greater ...19836298448
an immunologically active chimaeric protein containing herpes simplex virus type 1 glycoprotein d.herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) cause both persistent and latent infections, including recurrent cutaneous disease, lethal neonatal disease, central nervous system disease and other clinical syndromes. modified live vaccines or conventionally prepared subunit vaccines have generally been unsuccessful in the treatment of hsv-1 and hsv-2 infections from the standpoints of safety and efficacy. it has been established that hsv-1 and hsv-2 infectivity may be neutralized in vitr ...19836298636
is ribonucleotide reductase the transforming function of herpes simplex virus 2?transformation of cells by herpes simplex virus 2 (hsv-2) can be induced by the bglii c (0.43-0.58 map units) or n (0.58-0.625) fragments of the viral genome. sequences partially overlapping both fragments (0.566-0.602) encode two 3' coterminal mrnas; these in turn direct the synthesis of two related polypeptides of molecular weight 140,000 (140k) and 35k (refs 4, 7), which may be involved in transformation. recently, a temperature-sensitive (ts) mutation affecting hsv-induced ribonucleotide red ...19836298637
herpesvirus.herpesvirus (hsv) infection of the genital tract is a sexually transmitted disease that is increasing at an epidemic rate. 2 types of virus, type 1 (hsv-1) and type 2 (hsv-2) have been identified, of which hsv-2 is the major cause of genital and neonatal infection. type 2 herpes infections may be the 2nd most common venereal disease in the us. more than 60% of the adult us population has antibodies to hsv, and socioeconomic factors have been found to influence the incidence of hsv infection. ...19836301717
a comparative analysis of restriction enzyme digests of the dna of herpes simplex virus isolated from genital and facial lesions.the restriction endonuclease (re) cleavage patterns of the dna of herpes simplex virus (hsv) genital isolates from two geographically distinct groups and from one group of facial isolates were examined. eleven of 21 genital isolates from females, 1 of 27 genital isolates from males and all 17 of the facial isolates were hsv type 1 (hsv-1). the groups of isolates of the same serotype could not be distinguished by significant differences in the frequency of variable restriction endonuclease sites ...19836300289
herpes simplex virus. an expanding relationship to human cancer.herpes simplex virus (hsv) causes a wide spectrum of infectious disease in humans. during the past 15 years, evidence has accumulated indicating that, in addition, hsv may be a cause of human cancer, particularly cervical cancer. the research on hsv has taken five directions. first is detection of previous infection with hsv 2. this was the earliest approach and was performed largely with serologic assays. studies showed a higher incidence of infection with hsv-2 in cervical cancer patients as c ...19836300393
enzyme-linked immunosorbent assay for detection and typing of herpes simplex virus.an enzyme-linked immunosorbent assay for herpes simplex virus was tested using commercially available peroxidase-conjugated and unconjugated rabbit antibodies to herpes simplex virus type 1 and type 2 (dako immunoglobulins a/s, copenhagen, denmark). one hundred and thirty-seven clinical specimens from vesicles and superficial cutaneous lesions were tested and the results compared with virus isolation. in addition 210 herpes simplex virus isolates were typed. forty-four specimens yielded herpes s ...19836301810
viral genes modify herpes simplex virus latency both in mouse footpad and sensory ganglia.herpes simplex virus type 2 wild-type and 13 temperature-sensitive mutants have been examined for their ability to be recovered from latently infected biozzi mice. after inoculation into the rear footpad, virus could be recovered from both the dorsal root ganglia (drg) and the footpad (fp) at the site of inoculation (wt, ts 1, ts 2, ts 4, ts 6, ts 7 and ts 8), or from the drg only (ts 11 and ts 13), or from fp only (ts 3, ts 9, ts 12). two mutants (ts 5 and ts 10) have not been recovered from ei ...19836302212
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