| thymidine kinaseless revertants of ltk- cells transformed by herpes simplex virus type 1 are resistant to retransformation by homologous virus. | mouse l cells lacking thymidine kinase (ltk-) that had been transformed to the thymidine kinase-positive (tk+) phenotype by herpes simplex virus type 1 (hsv-1) were cultured in medium containing tritiated thymidine. six clonal lines of cells surviving this treatment were found to have the following properties: (i) the cells were tk- and had spontaneous back-reversion frequencies to the tk+ phenotype of 10(-5) or less, (ii) the cells contained hsv antigens, although in lesser amounts than in the ... | 1977 | 193795 |
| efficacy of herpes simplex virus type 1 immunization in protecting against acute and latent infection by herpes simplex virus type 2 in mice. | icr mice were immunized with herpes simplex virus type 1 (hsv-1) and later challenged with hsv-2 by footpad inoculation. both immunized animals and age-matched, nonimmunized controls were observed for ascending neurological disease and latent infection of spinal ganglia resulting from the hsv-2 challenge. control animals had a 78% incidence of acute and latent infection compared with a 1.7% incidence in immunized mice. the data show immunity to hsv-1 is protective against both acute and latent i ... | 1977 | 193798 |
| cell cycle dependency of murine cytomegalovirus replication in synchronized 3t3 cells. | synchronized murine 3t3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. the normal latent period of 12 h characteristic of asynchronous 3t3 cells was protracted to more than 24 h after an early g1 infection in synchronous cells. in this case viral progeny were not detected until after the initiation of the host s-phase. cells maintained in the g1 phase did not replicate virus. this failure could not be explained by a decrease ... | 1977 | 194054 |
| cell-mediated cytotoxicity to herpes-infected cells in humans: dependence on antibodies. | herpes simplex virus type 1 (hsv-1)-infected human skin fibroblasts were used as target cells in a 51cr release assay, using peripheral blood mononuclear cells from hsv-seropositive and -seronegative donors as effector cells. cytotoxicity was exerted by ordinarily prepared lymphoid cells but could be reduced by extensive washing of the effector cells. the antibody dependence of the system was shown by the recovery of activity through addition of positive serum or medium used for the early washes ... | 1977 | 194838 |
| effector cell involved in cell-mediated cytotoxicity to cells infected with herpes simplex virus type 1. | experiments were performed to analyze the characteristics of the effector cells involved in cell-mediated killing of herpes-infected fibroblasts. effector cells were fractionated according to adherence and phagocytic properties, and lymphocytes were depleted of t-cells, and ea- and eac- (antibody-coated bovine erythrocytes and bovine erythrocytes coated with rabbit anti-bovine antibodies and c5-deficient mouse serum, respectively) rosette-forming cells by differential centrifugation. column pass ... | 1977 | 194839 |
| regulation of herpesvirus macromolecular synthesis. vi. synthesis and modification of viral polypeptides in enucleated cells. | cells were enucleated with cytochalasin b after infection with herpes simplex virus 1. when protein synthesis was blocked by cycloheximide from the time of infection, mrna for viral alpha-infected cell polypeptides (icp) 4, 0, and 27 accumulated in the cytoplasm and was expressed after the removal of both drug and nucleus. a host protein, icp 22, whose synthesis is stimulated in intact cells, was not made, and viral protein icp4, which is normally modified to a form that migrates more slowly in ... | 1977 | 195087 |
| cell-free synthesis of herpes simplex virus proteins. | polyribosomes isolated from herpes simplex virus type i (hsv-1)-infected cells have been used to program a eucaryotic cell-free translation system. at least 10 hsv-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type hsv-infected polyribosomes. polyribosomes prepared from thymidine kinase-negative mutants of hsv direct the synthesis of three putative nonsense termination polypeptides. hsv-specific polypeptides synthesized in vitro are precipit ... | 1977 | 195089 |
| purification of herpesvirus nucleocapsids by fluorocarbon extraction. | purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with freon 113. fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of dna. cscl banded dna represented 30--40% of the theoretical yield of the total nucleocapsid population. | 1976 | 195183 |
| cellular immunity to herpes simplex virus in man. vii. k-cell activity to hsv1 infected target cells in disease. | herpes simplex virus-infected target cells, sensitised with antiviral antibody can be lysed by non-immune human lymphocytes (k-cells). this antibody dependent cell mediated immunity (adcc) is a very efficient technique for destroying foreign target cells and may play a role in maintaining the localization of recurrent herpetic infections. we have used 51cr labelled herpes virus infected target cells to examine the k-cell activity of peripheral blood lymphocytes in patients who have either malign ... | 1977 | 195708 |
| quantification of the herpes simplex virus dna present in biochemically transformed mouse cells and their revertants. | four cell lines biochemically transformed by u.v.-irradiated herpes simplex virus contain virus dna fragments ranging from 3 to 22% of the hsv genome. of five revertant clones selected for 3h-tdr or brdurd resistance, four had lost all detectable virus dna while the fifth, selected for brdurd resistance, retained the entire virus fragment but there was a reduction of virus copies per cell from 5 to 1. three 'supertransformed' revertant cell lines contained virus dna fragments ranging from 12 to ... | 1977 | 196037 |
| ammonium chloride inhibits cell fusion induced by syn mutants of herpes simplex virus type 1. | cell fusion induced by syn mutants of herpes simplex virus type 1 is inhibited by nh4cl. the inhibition of fusion is both rapid and rapidly reversible and apprears to be due to the nh4+ ion. virus production was not significantly altered by nh4cl, although cell growth was greatly diminished. | 1977 | 196105 |
| 5'-terminal and internal methylated nucleosides in herpes simplex virus type 1 mrna. | rna labeled with [methyl-3h]methionine and/or [32p]orthophosphate was isolated from the polyribosomes of herpes simplex virus (hsv) types 1-infected cells and separated into polyadenylylated [poly(a+)]and non-polyadenylylated [poly(a-)] fractions. virus-specific rna was obtained by hybridization in liquid to either excess hsv dna or filters containing immobilized hsv dna. analysis in denaturing sucrose gradients indicated that hsv-specific poly(a+) rna sedimented in a broad peak, with a modal s ... | 1977 | 196108 |
| anatomy of herpes simplex virus dna viii. properties of the replicating dna. | this paper concerns the properties of herpes simplex virus 1 dna replicating in hep-2 and human embryonic lung cells. the results were as follows. (i) only a small fraction of input viral dna entered the replicative pool. the bulk of the input viral dna cosedimented with marker viral dna and did not appear to be degraded or dissociated into l and s components. (ii) nascent dna sedimented faster and banded at a higher density than that of mature viral dna extracted from virions. pulse-chase exper ... | 1977 | 196115 |
| studies on the intracellular replicating dna of herpes simplex virus type 1. | | 1977 | 196402 |
| indirect immunofluorescence detection of human igm and igg antibodies against herpes simplex virus type 1 induced cell surface antigens. | an indirect immunofluorescence method, based on the use of infected hela cell coverslip cultures was developed to demonstrate human igm and igg class antibodies against herpes simplex virus (hsv) type 1 induced cell surface antigens. a total of 35 specimens from 20 patients have been tested; including patients with a clinical diagnosis of hsv type 1 or type 2 primary infection, patients with recurrent hsv infections, patients without any hsv infections, and patients with varicella-zoster virus ( ... | 1977 | 196479 |
| type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods. | human sera from patients showing seroconversion following a primary herpes simplex virus type 1 (hsv-1) or hsv type 2 (hsv-2) infection, as well as 19s and 7s fractions obtained from the same sera, have been used for typing 191 hsv isolates by an indirect peroxidase-labeled antibody (pla) method. typing has also been performed on all of the isolates using a microneutralization (mn) and the indirect hemagglutination (iha) inhibition test. furthermore, 30 isolates have been typed by a plaque metho ... | 1977 | 196572 |
| search for inhibitors against herpes simplex virus type-i in cell extracts derived from human lymphoblastoid cell lines. | cell extracts obtained from kb cells and 5 human lymphoblastoid cell lines including 2 from burkitt's lymphoma (p3hr-1 and raji), one each from nasopharyngeal carcinoma (no.223), acute lymphatic leukemia (molt-4) and a healthy person (nc-37) were tested for their inhibitory effects on the growth of herpes simplex virus type-1 (hsv-1) in green monkey kidney (gmk) cells by the plaque titration method. the relationship between the production of hsv-1 inhibitors and the degree of epstein-barr virus ... | 1977 | 196817 |
| protection from oral herpes simplex virus infection by a nucleic acid-free virus vaccine. | the effect of immunization with inactivated herpes virus vaccines, including a vaccine free of all nucleic acid, was investigated in a mouse model system. protection against oral lesions induced by herpes simplex virus type 1 was demonstrated by several criteria: (i) reduction in the incidence and severity of primary oral lesions; (ii) decrease in acute and latent infection of the regional sensory ganglia; and (iii) protection from viral encephalitis and death. the immune response of mice to the ... | 1977 | 197013 |
| effect of silica on the pathogenic distinction between herpes simplex virus type 1 and 2 hepatitis in mice. | the role of macrophages in the difference in liver pathogenicity between herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in mice was investigated by selectively blocking the macrophage function of the mice by silica. intravenous administration of 3 mg of silica 2 h before virus inoculation partially abolished the difference between the two virus types, as judged by macroscopic and microscopic examination of the livers and by virus isolation studies. intraperitoneal inoculation of 50 mg of ... | 1977 | 197016 |
| genetics of resistance to phosphonoacetic acid in strain kos of herpes simplex virus type 1. | a dna- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile dna polymerase activity, tsd9, was shown to be resistant to phosphonoacetic acid (paa) when plated at the permissive temperature. ts+ revertants of tsd9 were paa sensitive and exhibited dna polymerase activity intermediate between that of the wild-type virus and tsd9, indicating that both temperature sensitivity and sensitivity to paa are controlled by the same gene. since the position of tsd9 on the exist ... | 1977 | 197275 |
| in vitro transcription of herpes simplex virus ang dna by e-coli rna polymerase. | hsv-1 ang dna and a defective genome of the same virus were transcribed with e. coli rna polymerase under various salt conditions. the extent of transcription was assayed by hybridizing the crna to the hind iii, hpa i and hind ii restriction fragments of the dna templates using the blot technique of e. southern. the transcripts proved to contain sequences homologous to all dna fragments. a similar ratio of hybridized crna and the amount of fragment dna was observed in all cases. the results sugg ... | 1977 | 197492 |
| antibodies against herpes simplex virus type 1 subunit antigens in patients with trigeminal neuralgia and controls. | serum igg antibody levels against herpes simplex virus (hsv) type 1 subunit (capsid, envelope, and excreted) antigens detected with radioimmunoassay were compared in 25 patients with trigeminal neuralgia and their age- and sex-matched controls. no significant differences were found between the patients and controls, either in the distribution of the antibody titers or in the mean titers against any of the subunit antigens tested. in 6 patients hsv antibody titers were tested before and after tri ... | 1977 | 197767 |
| antiviral activity of arabinosylthymine in herpesviral replication: mechanism of action in vivo and in vitro. | 1-beta-d-arabinofuranosylthymine (ara-t), a metabolite of the sponge tethya crypta, has shown selective activity against herpes simplex virus (hsv) replication (g. a. gentry and j. f. aswell, virology 65:294-296, 1975). analysis of hsv-infected and uninfected cell lysates by cscl isopycnic centrifugation showed that ara-t blocked the incorporation of [(3)h]hypoxanthine into viral deoxyribonucleic acid and, to a large extent, into host deoxyribonucleic acid of infected (but not uninfected) cells. ... | 1977 | 197886 |
| temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genome. | | 1977 | 198141 |
| solid-phase enzyme immunoassay for herpes simplex virus. | an enzyme immunoassay was used for the rapid detection of herpes simplex virus type 1 (hsv-1). the test utilized specific antibodies to hsv-1 attached to a solid phase. a laboratory preparation of hsv-1 was detectable in amounts of greater than or equal to 50 50% tissue culture-infective doses by this method. tests performed with clinical samples indicated a specificity of 95%. the sensitivity appeared to depend on the length of time the samples were stored. when samples were stored frozen for t ... | 1977 | 198493 |
| anatomy of herpes simplex virus dna. ix. apparent exclusion of some parental dna arrangements in the generation of intertypic (hsv-1 x hsv-2) recombinants. | we are reporting the physical location of parental dna sequences in 28 recombinants produced by crossing herpes simplex viruses (hsv) 1 and 2. the parental crosses were of two kinds. in the first, temperature-sensitive mutants of hsv-1 and hsv-2 were crossed to produce wild-type recombinants. in the second, temperature-sensitive mutants of hsv-1 rendered resistant to phosphonoacetic acid were crossed with wild-type hsv-2, and recombinants that multiplied at nonpermissive temperature and were res ... | 1977 | 198577 |
| [125i]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase. | [125i]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (tk), whereas [125i]deoxycytidine was a very poor substrate for cellular tk. simple, sensitive, and specific assays for viral tk could be carried out in vivo and in vitro even in the presence of cell tk. autoradiographic detection of incorporated [125i]deoxycytidine provided a rapid and simple method for detection of and screening for viral tk in infected as well as viral tk-transformed cells. | 1977 | 198581 |
| treatment of fatal disseminated herpes simplex virus, type 1, infection in immunosuppressed mice (39899). | | 1977 | 198818 |
| [herpetic infection of the oral cavity in guinea pigs]. | a convenient experimental model for serial studies of herpes simplex of the buccal mucosa has been developed in guinea pigs by application of fresh isolates of herpes simplex virus type 1 from a patient with primary herpetic gingivo-stomatitis on prescarified buccal mucosa. the herpetic nature of the eruptions in the oral cavity was confirmed by virus isolations and serologically. the possibility of exogenous reinfection of the buccal mucosa in convalescent animals against the background of humo ... | 1977 | 198963 |
| specifically immune mouse t-cells can destroy h-2 compatible murine target cells infected with herpes simplex virus types 1 or 2. | lymphocytes from the spleen (sl) or peritoneal cavities (pl) of hsv-1- or hsv-2-immunized c 3 h mice expressed pronounced cytotoxicity, as measured by 51cr release assay, against l cells experimentally infected with hsf-types 1 or 2 but not against uninfected l cells. hsv-1 or hsv-2 immune lymphocytes induced substantially more 51cr release when l-cell targets were infected with homotypic virus compared to those infected with heterotypic virus. inasmuch as the cytotoxicity of specifically immune ... | 1977 | 198986 |
| comparison of igg level and fluorescent antibody titre to herpes simplex virus type 1 in cerebrospinal fluid and serum of encephalitis patients and controls. | | 1977 | 199683 |
| herpes simplex virus dna polymerase as the site of phosphonoacetate sensitivity: temperature-sensitive mutants. | temperature-sensitive (ts) mutants in a number of complementation groups of herpes simplex virus type 1 (hsv-1) are deficient in dna polymerase induction at the restrictive temperature. twenty-two mutants in 15 complementation groups were tested for sensitivity to phosphonoacetate (paa), a compound that inhibits hsv replication in vivo and the dna polymerase in vitro. one mutant, tsd9, was resistant to paa (pr), whereas all others were sensitive. revertants of tsd9 to the ts+ phenotype simultane ... | 1977 | 199734 |
| alterations of neutral glycolipids in cells infected with syncytium-producing mutants of herpes simplex virus type 1. | the isolation of syncytium-producing mutants of herpes simplex virus type 1 (kos strain), which cause extensive cell fusion during otherwise normal infections, has been reported previously (s. person, r. w. knowles, g. s. read, s. c. warner, and v. c. bond, j. virol. 17:183-190, 1976). seven of these mutants, plus two syncytial strains obtained elsewhere, were used to compare the incorporation of labeled galactose into neutral glycolipids of mock-infected, wild-type-infected, and syncytially inf ... | 1977 | 199742 |
| studies on the in vivo methylation of replicating herpes simplex virus type 1 dna. | | 1977 | 199990 |
| inhibition of antigen and mitogen-induced lymphocyte transformation by fetal calf serum. | use of fetal calf serum as a serum supplement in whole blood microcultures of human lymphocytes resulted in a significant suppression of in vitro stimulation with herpes simplex virus (type 1) antigen, purified protein derivative, and phytohemagglutinin. if the response to viral antigen is weak in autologous serum it may be completely missed if cultures are carried out in fetal calf serum. | 1977 | 200328 |
| scanning electron microscopy of the surfaces of hamster embryo cells transformed by herpes simplex virus. | the surfaces of normal hamster embryo fibroblast (hef) cells were examined by scanning electron microscopy. surface characteristics of hef cells were compared to those of cells derived from a primary tumor induced in hamsters following s.c. inoculation of herpes simplex virus type 1-transformed hef cells (14-012-8-1) and to the surfaces of cells derived from a metastatic tumor to the lung induced by the same cells. the most obvious difference in the surface characteristics of the examined cells ... | 1977 | 200348 |
| interaction between cyclic nucleotides and herpes simplex viruses: productive infection. | infection of human fibroblasts and hep-2 cells with herpes simplex virus type 1 (hsv-1) produced a decrease in the intracellular levels of cyclic adenosine 5'- monophosphate (camp) and a concomitant increase in the cyclic guanosine 5'- monophosphate (cgmp) levels. in both cell cultures, changes in cyclic nucleotide levels were first observed at 6 h after viral inoculation and were maximal at 12 h. in human fibroblasts, the addition of theophylline, dibutyryl camp, or papaverine (camp-enhancing c ... | 1977 | 200559 |
| [herpes simplex virus (hsv-1 and hsv-2) infection: its clinical and oncogenic properties (author's transl)]. | the epidemiology and clinical features of diseases caused by the herpes simplex virus, are reviewed and recent results are discussed which give an insight into the complex mechanism of primary and chronic, recurrent hsv-1 infections. immunological reactions in hsv infection and data concerning the oncogenic potential of hsv-1 and -2 are dealt with. furthermore, current therapeutic possibilities are outlined. | 1977 | 201107 |
| detection of herpes simplex virus thymidine kinase polypeptides in cells labeled with 35s-methionine. | to investigate the size of herpes simplex virus (hsv) thymidine kinase (tk) polypeptides, procedures have been devised to purify the enzyme from infected cells labeled with 35s-methionine by (i) affinity chromatography on sepharose-5'-amino-5'-deoxythymidine; (ii) preparative isoelectric focusing or preparative page; and (iii) glycerol gradient centrifugation. portions of enzyme fractions, at each purification step, were also treated with an immunoadsorbent, sepharose-anti-hsv-1 tk immunoglobuli ... | 1978 | 201586 |
| psychopharmaca and electroconvulsive therapy in relation to viral antibodies and interferon. experimental and clinical study. | chlorpromazine (chp) in a concentration of 8.3 microgram per ml medium inhibited attachment and multiplication of cec and of various cell lines. the same chp concentration applied to grown cec monolayers inhibited the growth of tbev but not the growth of hhv 1, while interferon was slightly stimulated. the clinical course of fatal subcutaneous tbev infection in mice was not affected by daily chp treatment while brain and serum interferon were stimulated. antibody formation in tbev infected or hh ... | 1977 | 202214 |
| inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants. | herpes simplex virus type 1 dna synthesis and infections progeny production were studied in five different conditional hamster (bhk-21) cell cycle mutants. at the nonpermissive temperature (39.5 degrees c), both events were strongly inhibited in four of these cell lines. the degree of inhibition was a reproducible characteristic of each cell mutant and in two cases was dependent upon the multiplicity of infection. experiments involving shifts to the nonpermissive temperature at least 3 h postinf ... | 1978 | 202752 |
| radiobiological inactivation of epstein-barr virus. | lymphocyte transforming properties of b95-8 strain epstein-barr virus (ebv) are very sensitive to inactivation by either uv or x irradiation. no dose of irradiation increases the transforming capacity of ebv. the x-ray dose needed for inactivation of ebv transformation (dose that results in 37% survival, 60,000 rads) is similar to the dose required for inactivation of plaque formation by herpes simplex virus type 1 (fischer strain). although herpes simplex virus is more sensitive than ebv to uv ... | 1978 | 202757 |
| selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine. | a guanine derivative with an acyclic side chain, 2-hydroxyethoxymethyl, at position 9 has potent antiviral activity [dose for 50% inhibition (ed(50)) = 0.1 mum] against herpes simplex virus type 1. this acyclic nucleoside analog, termed acycloguanosine, is converted to a monophosphate by a virus-specified pyrimidine deoxynucleoside (thymidine) kinase and is subsequently converted to acycloguanosine di- and triphosphates. in the uninfected host cell (vero) these phosphorylations of acycloguanosin ... | 1977 | 202961 |
| variation of dna polymerase and rna polymerase activities in cells infected with herpes simplex virus type 1. | | 1978 | 203094 |
| selective inhibition of herpes simplex virus type 1 dna polymerase by zinc ions. | | 1978 | 203099 |
| [in vitro studies of the antiviral activity of ammonium humate against herpes simplex virus type 1 and type 2 (author's transl)]. | ammonium humate, isolated from peat water, is a higher molecular polyphenolic compound with a strong antiviral activity against herpes simplex virus type 1 and type 2. three cell lines were chosen for examining the cytotoxicity of ammonium humate: rabbit kidney primary cells, hep-2- and fl-cells. as the light microscopic examination of both, the monolayers and the stained cover-glass cultures, revealed, concentrations of up to 5 microgram/ml ammonium humate can be used without hesitation for rab ... | 1977 | 203143 |
| herpes simplex virus types 1 and 2 in ocular disease. | in a continuous series of 457 patients with presumed herpetic eye disease, virus isolation and typing revealed 154 patients with herpes simplex virus type 1 and three patients with type 2 infections. in 219 isolates that were examined for neutralization by specific antisera, for growth in human fibroblasts, and, in part, for temperature sensitivity, there were found substantial strain differences in addition to the type-specific characteristics. the clinical features of each of the three type 2 ... | 1978 | 203255 |
| association of thymidylate kinase activity with pyrimidine deoxyribonucleoside kinase induced by herpes simplex virus. | thymidine kinase derived from lmtk+ does not exhibit thymidylate kinase activity. however, protein isolated by affinity column chromatography from thymidine kinase-deficient mouse cells (lmtk-) infected by herpes simplex virus type 1 shows thymidylate kinase activity in addition to thymidine kinase and deoxycytidine kinase activities. the virus-induced multifunctional enzyme has a molecular weight of 85,000, whereas the molecular weight of thymidylate kinase from uninfected lmtk- mouse cells is ... | 1978 | 203589 |
| radiometric detection of herpes simplex viruses. | two radiometric techniques were investigated as possible means of detecting viruses in clinical specimens. the effect of herpes simplex virus on dna synthesis by monolayers of human embryonic lung fibroblasts was monitored by the incorporation of [3h] thymidine or [125i] iododeoxyuridine ([125i] idu). radiometric results were compared with those obtained from visual examination for cytopathic effects in the same cell line. cells infected with herpes simplex virus type-2 (hsv-2, 10(6.8) virions) ... | 1978 | 203666 |
| deoxyribonucleic acid polymerase of wild-type and phosphonoacetic acid-resistant mutant of herpes simplex virus. | the phosphonacetic acid (paa)-susceptible deoxyribonucleic acid (dna) polymerase of herpes simplex virus type 1 was partially purified and isolated in sucrose gradients and on double-strand dna cellulose columns. the dna polymerase isolated from cells infected with the paa-resistant mutant had the same molecular weight as the wild-type enzyme (140,000 to 149,000) but was consistently more resistant to paa. | 1978 | 204249 |
| incidence and distribution of herpes simplex virus types 1 and 2 from genital lesions in college women. | during a 9-month period, 9,772 women were treated at the student health center's gynecology clinic. herpes simplex virus was isolated from 30 of 57 patients clinically diagnosed as suffering from a herpetic or herpetic-like genial infection for a virological incidence rate of 0.31%. using virus plaque diameter in chick embryo cells and heat stability of viral thymidine kinase, 37% of the isolates were classified as herpes simplex virus type 1 and 63% were classified as herpes simplex virus type ... | 1977 | 204741 |
| induction capacity and influence of dthdmp on thymidine kinase activity of type 1 and 2 strains of herpes simplex virus. | the thymidine kinase inducing ability of 104 strains of herpes simplex virus was studied comparatively. a pronounced relationship was established between induction of the enzyme and the serotype of the strains. as a rule, the strains of serotype 2 are weaker inducer of dthd- and dcyd-kinase activity than serotype 1 strains. a certain parallelism exists between induction of both enzymes, however the activity of the thymidine kinase increases after infection with herpes simplex virus 4--5 times mo ... | 1978 | 205197 |
| isolation of herpes simplex virus type 1 from lesions of erythema multiforme. | | 1978 | 205333 |
| the fate of parental herpesvirus dna after infection with normal and photoinactivated virions. | the fate of the 3h-dt-labelled parental dna of normal and photoinactivated herpes simplex virus type 1 (kos strain) was followed for 8 h after infection. the sedimentation coefficients of parental virus dna from cells infected with normal virons increased and became associated with the replicative intermediates of hsv-1 dna. when cultured in 5-bromodeoxyuridine-containing medium, the buoyant density of the normal parental virus dna shifted to the hybrid region (containing heavy and light molecul ... | 1978 | 205628 |
| physical map of the origin of defective dna in herpes simplex virus type 1 dna. | the origin of defective dna (ddna) of the patton strain of herpes simplex virus type 1 (hsv-1) was physically mapped with bamhi in the parental dna. the ddna obtained from virus passaged at high multiplicities of infection was resistant to cleavage with hindiii, whereas digestion with ecori yielded a cluster of fragments 5.4 to 5.7 megadaltons (mdal) in size. cleavage with bamhi gave a cluster of fragments 2.6 to 3.2 mdal in size, plus two homogeneous, comigrating 1-mdal fragments. one of the la ... | 1978 | 205677 |
| herpes virus infection alters electrical parameters of heart cell aggregates. | the spontaneous beat of embryonic heart cell aggregates in culture grew gradually weaker and stopped about 18 h after inoculation with herpes simplex virus 1 (hsv-1). before electrical activity ceased, maximal action potential upstroke velocity fell to about 20% of control values, whereas overshoot and plateau duration declined about 50%. maximal diastolic potential was reduced by only about 10%. eighteen hours after viral inoculation, specific membrane resistance and intercellular coupling were ... | 1978 | 206155 |
| the deoxyribonuclease induced after infection of kb cells by herpes simplex virus type 1 or type 2. i. purification and characterization of the enzyme. | the deoxyribonuclease induced in kb cells by herpes simplex virus (hsv) type 1 and type 2 has been purified. both enzymes are able to completely degrade single- and double-stranded dna yielding 5'-monophosphonucleotides as the sole products. a divalent cation, either mg2+ or mn2+, is an absolute requirement for catalysis and a reducing agent is necessary for enzyme stability. the maximum rate of reaction is achieved with 5 mm mgcl2 for both hsv-1 and hsv-2 dnase. the optimum concentration for mn ... | 1978 | 206546 |
| a host range difference between herpes simplex virus types 1 and 2. | the infectivities of several strains of herpes simplex virus type 1 and type 2 were measured by plating on established human and chinese hamster cell lines. whereas all the type 1 strains reproducibly showed a 10(4)-fold lower titre on the chinese hamster cells, the type 2 strains showed only a 10- to 100-fold lower titre. this host range difference has been used in the analysis of recombinants between type 1 and type 2 viruses. | 1978 | 206659 |
| interaction of ultraviolet-irradiated herpes simplex virus type 1 with bsc-1 cells. | ultraviolet irradiation of herpes simplex virus (hsv) did not affect the transfer of uncoated virus dna to the nuclei of infected cells but the synthesis of virus dna was suppressed. the virus-specific dna polymerase was synthesized in cells infected with the u.v.-irradiated hf strain of hsv. in cells infected with the u.v.-irradiated kos strain, the virus dna polymerase activity was hardly detectable. the two strains of hsv differ in the sensitivity of the virus dna polymerase gene to u.v.-irra ... | 1978 | 206661 |
| deoxyadenosine antagonism of the antiviral activity of 9-beta-d-arabinofuranosyladenine and 9-beta-d-arabinofuranosylhypoxanthine. | deoxyadenosine but not adenosine reversed the antiviral activity of 9-beta-d-arabinofuranosyladenine (ara-a) and 9-beta-d-arabinofuranosylhypoxanthine (ara-h) when used in the presence of coformycin, an inhibitor of adenosine deaminase. in suspension cultures of kb cells, 10 mum ara-a inhibited the replication of herpes simplex virus type 1 by 80%. concomitant addition of 50 mum deoxyadenosine reduced the antiviral activity of 10 mum ara-a to only 40% inhibition. adenosine failed to antagonize t ... | 1978 | 207416 |
| introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus dna or transformed cell dna. | cells lacking the enzyme thymidine kinase (lmtk- cells) have been transformed to a kinase-positive phenotype using sheared herpes simplex virus (hsv) dna, and the enzyme found in these transformed cells is hsv-specific. one of the cell lines is able to complement the functional defect found in two temperature-sensitive mutants of hsv 1, and reversion of the cells to a thymidine kinase-negative phenotype results in the loss of this capability. the hsv thymidine kinase gene can also be introduced ... | 1978 | 207433 |
| alteration of the growth of cytomegalovirus and herpes simplex virus type 1 by epidermal growth factor, a contaminant of crude human chorionic gonadotropin preparations. | pretreatment (12-48 h) of human fibroblasts with crude, human chorionic gonadotropin (hcg) was found to suppress cytomegalovirus infection and enhance productive herpes simplex type 1 (hsv) infection in vitro. maximal effect on virus replication occurred at the time of maximal infectivity of control cultures (48 h and 6 days after viral innoculation for hsv and cytomegalovirus, respectively). the alteration in viral growth was not due to the hcg itself, but rather to epidermal growth factor, a c ... | 1978 | 207740 |
| salivary neutralizing activity against herpes simplex virus type 1. | samples of saliva collected from 40 patients, of whom 16 had a previous clinical history of herpes simplex virus type 1 (hsv-1) infection, were concentrated and sterilized. salivary neutralizing activity, as measured by a plaque-reduction method, was found in 62% of those patients with a history of oral lesions. studies with antisera to human immunoglobulin indicated that the igg class of immunoglobulin was a major hsv-1-neutralizing component of both saliva and serum. | 1978 | 207786 |
| synthesis of viral and host dna in isolated chromatin from herpes simplex virus-infected hela cells. | dna synthesis in chromatin isolated from herpes simplex virus type 1-infected hela cells (hsv chromatin) was examined in vitro. the hsv chromatin was found to carry out an initial limited synthesis of dna in vitro, 50 to 64 pmol of dtmp incorporated in 10(6) nuclei per 10 min, which is comparable to that found in nuclei isolated from hsv-infected cells. dna synthesis in vitro proceeded for only 30 min, and both hsv dna and host dna were synthesized in significant amounts. the hsv and host dna sy ... | 1978 | 207889 |
| anatomy of herpes simplex virus (hsv) dna. x. mapping of viral genes by analysis of polypeptides and functions specified by hsv-1 x hsv-2 recombinants. | in an earlier paper (morse et al., j. virol 24:231--248, 1977) we reported on the provenance of the dna sequences in 26 herpes simplex virus type 1 (hsv-1) x hsv-2 recombinants as determined from analyses of their dnas with at least five restriction endonucleases. this report deals with the polypeptides specified by the recombinants and by their hsv-1 and hsv-2 parents. we have identified (i) the corresponding hsv-1 and hsv-2 polypeptides with molecular weights ranging from 20,000 to more than 2 ... | 1978 | 207894 |
| intramembrane changes occurring during maturation of herpes simplex virus type 1: freeze-fracture study. | during the maturation of two strains of herpes simplex virus type 1 (vr3 and patton), intramembrane changes were detected with the freeze-fracture technique in the viral envelope and the infected cell plasma membrane, and these changes were compared with data obtained from thin sections. regardless of the strain, the inner leaflet of the viral envelope of extracellular virions was characterized by a density of intramembrane particles (imp) three times larger than the host nuclear and plasma memb ... | 1978 | 207896 |
| amplification of two endo r-hind iii-restricted fragments of the dna of herpes simplex virus type 1. | | 1978 | 208239 |
| analysis of herpes simplex virus type 1 antigens exposed on the surface of infected tissue culture cells. | | 1978 | 208278 |
| comparison of hsv1 dna-induced and spontaneous transformation of hamster cells. | primary cultures of hamster embryo fibroblasts were transfected by purified, fragmented herpes simplex virus type 1 (hsv1) strain a44 dna in the presence of carrier dna. control cultures were transfected with carrier dna only. a cell line transformed by hsv1 dna (eh/a44 cell line) was established after serial passages, without senescent crisis. a control cell line (eht cell line), spontaneously transformed in vitro, was established after a 13th passage crisis. both cell lines originated from the ... | 1978 | 208448 |
| 5,6-dihydro-5-azathymidine: in vitro antiviral properties against human herpesviruses. | 5,6-dihydro-5-azathymidine (dhadt), a novel water-soluble nucleoside antibiotic, inhibits herpes simplex virus type 1 (hsv-1) in appropriately infected cell cultures to a greater extent than herpes simplex virus type 2 (hsv-2). vaccinia virus was less susceptible than hsv-2, and pseudorabies virus yields were not reduced at the concentrations studied. plaque formation by varicella-zoster virus was suppressed by dhadt. dhadt was slightly toxic to cells at concentrations that were inhibitory for h ... | 1978 | 208459 |
| antiviral activity of some beta-diketones. 3. aryl bis(beta-diketones). antiherpetic activity. | a series of bis(beta-diketones) was synthesized and tested in vitro for antiviral actitity against herpes simplex type 2. two parameters which were studied in an effort to optimize activity were the nature of the aryl group and the length of the alkyl bridge. one of the more active compounds, 4,4'-[(1,4-phenylenedioxy)bis(6,1-hexanediyl)]-bis[3,5-heptanedione] (6), was evaluated more extensively and found to inhibit the cytopathic effect in tissue culture of herpes simplex virus type 1 as well a ... | 1978 | 209188 |
| nucleoside 5'-monophosphate analogues. synthesis of 5'-sulfamino-5'-deoxynucleosides. | the synthesis of two new nucleotide analogues is described. 5'-sulfamino-5'-deoxyadenosine (1) was prepared by reaction of 5'-amino-5'-deoxyadenosine with (ch3)3n.203, and 5'-sulfamino-5'-deoxythymidine (2) was prepared from 5'-amino-5'-deoxythymidine by a similar reaction. the 5'-sulfamino nucleosides are shown to be quite stable to hydrolysis in acidic or basic aqueous solution tests show that these compounds do not inhibit the growth of escherichia coli or l1210 cells at concentrations less t ... | 1978 | 209190 |
| protective effect of an oral infection with herpes simplex virus type 1 against subsequent genital infection with herpes simplex virus type 2. | the problem of whether oral herpes simplex virus type 1 (hsv-1) infection provides protection against subsequent genital infection by herpes simplex virus type 2 (hsv-2) was investigated. mice were used as models. following conditions in man, both the oral and genital infections applied were noninjurious. mice infected orally with hsv-1 were weakly protected against virus 'take' following vaginal challenge with hsv-2. genital 'takes' were found in 67% of the immunized mice, as compared with 83% ... | 1978 | 209295 |
| host-cell reactivation of uv-irradiated and chemically-treated herpes simplex virus-1 by xeroderma pigmentosum, xp heterozygotes and normal skin fibroblasts. | | 1978 | 209320 |
| isoenzymes of lactate dehydrogenase, acid phosphatase, alkaline phosphatase and peroxidase in monkey kidney cell cultures inoculated with herpes virus type 1. | the variation of lactate dehydrogenase, peroxidase, acid and alkaline phosphatase isoenzymes was studied in vero cells inoculated with infectant and uv-inactivated herpes simplex virus type 1 (hsv--1). infectant hsv--1 induced quantitative and qualitative modifications in isoenzyme patterns within the first 4 hours post inoculation (p.i.). the modifications caused by the uv-inactivated hsv--1 were similar, but appeared 8 hours p.i. the possibility of using isoenzyme modifications as rapid, sensi ... | 1978 | 209614 |
| synthesis and packaging of herpes simplex virus dna in the course of virus passages at high multiplicity. | herpes simplex virus type 1 (hsv-1), strain ang, was passaged serially at high moi or undiluted on rc-37 cells. the yields of infectious virus decreased and increased periodically with a maximum of about 3 log units variation in titer. the amount of newly synthesized hsv-1 dna, however, did not vary by more than a factor of 2. on the other hand, the fraction of total hsv-1 dna associated with virions was reduced at passage numbers coinciding with the minima in the yields of infectious progeny vi ... | 1978 | 210139 |
| effect of immunization on the development of latent ganglionic infection in mice challenged intravaginally with herpes simplex virus types 1 and 2. | immunization of balb/c mice with virulent and avirulent strains of hsv-1 resulted in high levels of neutralizing antibody and protected against both the lethal effect of the virus and the development of a latent ganglionic infection when animals were challenged by the intravaginal route. in animals immunized with avirulent strain of hsv-2 and challenged with a high virulent strain of hsv-2, substantial protection against death was observed despite low levels of neutralizing antibody. nineteen pe ... | 1978 | 210669 |
| complement-mediated cytolysis of hsv-1 and hsv-2 infected cells: plasma membrane antigens reactive with type-specific and cross-reactive antibody. | surface antigens of bhk-21 cells infected with hsv-1 or hsv-2 were radioiodinated (125i) with lactoperoxidase, immune precipitated and analysed by polyacrylamide gel electrophoresis (page). experiments using antiserum to hsv-1 or hsv-2, absorbed with appropriate hemotypic or heterotypic antigens, revealed that both type-specific (homotypic) and cross-reactive antibody combined with surface glycoproteins to form a single large radioactive peak. this peak, which constituted the major glycoprotein ... | 1978 | 211189 |
| herpes simplex virus type-1 and human lymphocytes: virus expression and the response to infection of adult and foetal cells. | the growth of herpes simplex virus type i (hsv-i) in human lymphocytes of adult and foetal origin was studied. virus dna synthesis, antigen and particle production and the yield of infectious progeny were determined in cultured lymphocytes with or without exposure to stimulating concentrations of the mitogens phytohaemagglutinin and pokeweed mitogen. separated sub-populations of cells were examined and the conclusion reached that only the stimulated t-lymphoblast was permissive for full virus ex ... | 1978 | 211196 |
| herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. i. light microscopic observations of myelin degeneration. | mature mouse spinal cord-ganglion cultures, which contain both peripheral and central nervous system as one unit, were infected with herpes simplex virus type 1 (hsv 1) or type 2 (hsv 2) and observed by bright field microscopy for up to 72 hours. there was degeneration of both central and peripheral myelin in cultures infected with either virus, but the pattern of peripheral myelin degeneration associated with hsv 1-infected cultures was differrnt from that in hsv 2-infected cultures. type 1 was ... | 1978 | 211204 |
| anatomy of bovine mammillitis dna ii. size and arrangements of the deoxynucleotide sequences. | we previously reported that bovine mammillitis virus (bmv) dna consists of two covalently linked components designated l and s and estimated to be 71.5 x 10(6) and 15.7 x 10(6) in molecular weight, respectively; the components invert relative to each other, giving rise to four equimolar populations differing soley in the relative orientation of the two components. we now report that (i) bmv dna has a contour length corresponding to a molecular weight of 89 x 10(6). (ii) component l consists of a ... | 1978 | 211253 |
| mapping early transcripts of herpes simplex virus type 1 by electron microscopy. | rna displacement loop patterns in intact herpes simplex virus dna and herpes simplex virus dna restriction fragments indicate that viral rna associated with polyribosomes early after infection is transcribed from three major areas of the genome. one area of early transcription is in the short segment of the viral dna and is roughly delineated by the inverted repeat sequences bounding this segment. the other two areas of early mrna transcription map in the long segment. each of three major areas ... | 1978 | 211255 |
| evidence for a herpes simplex virus-specific factor controlling the transcription of deoxypyrimidine kinase. | a cistron-specific, enzyme-forming-capacity method was used to study the control of herpes simplex virus (hsv)-specific deoxypyrimidine kinase (dpyk) mrna synthesis. a virus-specific factor was formed by a primary infecting virus, and this factor effected the transcription of dpyk mrna of a superinfecting virus in the presence of cycloheximide, suggesting that the factor acted in "trans" and was a diffusible one. after the addition of actinomycin d to prevent further transcription and upon remov ... | 1978 | 211259 |
| structure of the joint region and the termini of the dna of herpes simplex virus type 1. | analysis of restriction endonuclease cleavage sites within the inverted, repeated sequences in the joint region of the dna of herpes simplex virus type 1 strain kos revealed the presence of two types of sequence heterogeneity. the first was an insertion of 280 base pairs or multiples of 280 base pairs which was found in approximately half of all dna molecules from every plaque-purified stock of virus. these insertions seemed to be tandem duplications of sequences which were present at the joint ... | 1978 | 211266 |
| cellular immune response in genital herpes simplex virus infection. | we studied the relations between the cellular immune response, pre-existing complement-fixing antibody and virus type with duration of virus excretion in genital herpes simplex virus (hsv) infection. thirty-six patients (seven with hsv-1 and 29 with hsv-2) with genital herpes underwent serologic testing, sequential viral cultures and weekly determination of lymphocyte-transformation stimulation index with inactivated hsv antic n. the duration of virus excretion was shortest in those with pre-exi ... | 1978 | 211414 |
| molecular genetics of herpes simplex virus: demonstration of regions of obligatory and nonobligatory identity within diploid regions of the genome by sequence replacement and insertion. | the dnas of herpes simplex virus (hsv) 1 and 2 consist of two components, l and s, each composed of unique sequences bracketed by inverted repeats. in this study we have probed the structure of the reiterated regions of the s component in marker rescue experiments involving transfection of cells with mixtures of intact hsv-1 mutant viral dna and individual dna fragments generated by restriction endonuclease digestion of wild-type hsv-1 or hsv-2 dnas. the results were as follows: (i) hsv is diplo ... | 1978 | 211508 |
| in vitro rna synthesis in nuclei from herpes simplex virus infected cells: effects of ultraviolet-irradiated, defective and bromodeoxyuridine-resistant virus preparations. | transcription of virus dna was detectable in vitro in nuclei isolated from cells infected with wild type and bromodeoxyuridine (budr)-resistant herpes simplex virus type 1 but was suppressed in nuclei of cells infected with ultraviolet irradiated or defective virus or cells treated with budr during infection. | 1978 | 211993 |
| the pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. | the pathogenicity for mice of two mutants of herpes simplex virus (type 1 and type 2), which fail to induce thymidine kinase, were compared with their respective parent strains. the mutants were much less virulent than the parents following either intracerebral or peripheral inoculation. the replication of the virus at the site of inoculation and its progression into the nervous system were studied. following a very large inoculum in the ear, the type 1 mutant was found to establish a latent inf ... | 1978 | 212476 |
| suppression of the synthesis of cellular macromolecules by herpes simplex virus. | synthesis of cellular protein was substantially inhibited within 1 h of infection with herpes simplex virus, type 2, strain g (hsv-2). the inhibition also occurred, although no virus-specific protein synthesis was detected, after infection with u.v. irradiated virus and in cytoplasts that had been enucleated before infection. the inhibitory activity could not be distinguished from infectivity by dilution, sedimentation or reaction with gamma-globulin. hsv-2 also suppresssed the synthesis of send ... | 1978 | 212520 |
| steroid hormone alteration of herpes simplex virus type 1 replication. | the effect of steroid hormones on herpes simplex virus type 1 replication was examined. virus replication studies revealed that various concentrations of prednisolone, hydrocortisone, dexamethasone, or progesterone could decrease virus yields up to a maximum of 99%. using isopycnic centrifugation in cscl to separate viral from cell dna, it was found that virus-specific dna synthesis was decreased by 30 to 100% depending on the hormone and concentration used. cell-specific dna synthesis was also ... | 1978 | 212528 |
| collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2. | twenty-three complementation groups of herpes simplex virus type 1 (hsv-1) and 20 of hsv-2 were identified by qualitative and quantitative complementation analysis from among 43 temperature-sensitive (ts) mutants of hsv-1 and 29 ts mutants of hsv-2 which had been isolated independently in 10 laboratories. | 1978 | 212578 |
| timing of some of the molecular events required for cell fusion induced by herpes simplex virus type 1. | the timing of some of the molecular events that are required for cell fusion was investigated. cell fusion was produced by a mutant of herpes simplex virus type 1 that causes extensive cell fusion during infection. the timing of molecular events required for fusion was established by the use of blocking agents. phosphonoacetic acid blocks viral dna synthesis; actinomycin d blocks rna synthesis; cycloheximide blocks protein synthesis; 2-deoxyglucose blocks glycosylation of glycoproteins; high tem ... | 1978 | 212579 |
| requirement of protein synthesis for the degradation of host mrna in friend erythroleukemia cells infected wtih herpes simplex virus type 1. | we describe experiments which demonstrate that shortly after infection of friend erythroleukemia cells with herpes simplex virus (hsv), polyribosomes dissociate and cellular mrna degrades. analysis of infected cell extracts on sucrose density gradients demonstrates that the majority of the polyribosomes have dissociated to monoribosomes at 2 h postinfection. physical measurements of infected-cell rnas support this conclusion and demonstrate that the polyadenylated rnas decrease in size. the degr ... | 1978 | 212585 |
| viral dna synthesis in cells infected with temperature-sensitive mutants of herpes simplex virus type 1. | temperature-sensitive mutants of herpes simplex virus type 1 representing eight dna-negative complementation groups were grouped into the following three categories based on the viral dna synthesis patterns after shift-up from the permissive to the nonpermissive temperature and after shift-down from the nonpermissive to the permissive temperature in the presence and absence of inhibitors of rna and protein synthesis. (i) viral dna synthesis was inhibited after shift-up in cells infected with tsb ... | 1978 | 212598 |
| physical mapping of herpes simplex virus type 1 mutations by marker rescue. | a generally applicable technique which permits the rescue of selected genetic markers from fragments of herpes simplex virus dna is described. baby hamster kidney cells infected at the nonpermissive temperature with intact dna from temperature-sensitive mutants or with fragmented wild-type dna produce no, or little, infectious progeny. coinfection results in an increased yield of virus, demonstrating the rescue of genetic information from the dna fragments. this progeny virus consists of both wi ... | 1978 | 212602 |
| comparison of the development of resistant strains of type 1 herpes simplex virus to in vitro antiviral activity of 5-iodo-2'-deoxyuridine or ribavirin. | exposure of hsv/1 to low concentrations of ribavirin during 4--5 passages does not produce ribavirin-resistant virus. idu resistance was developed by hsv/1 while it was being passed simultaneously. this resistance was seen to develop in both kb and vero cells. the idu-resistant virus is also resistant to ribavirin in kb cells. | 1977 | 212974 |
| inhibition of herpesvirus dna synthesis by 9-beta-d-arabinofuranosyladenine in cellular and cell-free systems. | 9-beta-d-arabinofuranosyladenine 5'-triphosphate (ara-atp) is an inhibitor both of dna polymerase-alpha and -beta from noninfected rabbit kidney cells and of the dna-dependent dna polymerase induced by herpes simplex virus type 1 (strain ies). the studies were performed with partially purified enzymes, and each of the different polymerase preparations contained only one dna-dependent dna polymerase species. these enzymes were inhibited in a competitive manner. the hsv-induced dna-dependent dna p ... | 1977 | 212980 |
| arabinosyl-n6-hydroxyadenine: a new potent antivirus drug. | abrabinosyl-n6-hydroxyadenine (ara-ha) was tested for its antiherpesvirus and immunosuppressive activities in vivo and in vitro. mouse strains were used that had been shown earlier to be very susceptible to intraperitoneal (i.p.) or intracerebral (i.c.) inoculation of a virulent strain of herpes simplex virus type 1 (hsv-1). susceptible mice were inoculated i.p. or i.c. with a stock pool of hsv-1 and treated with ara-ha (i.p.) beginning at least 24 hr later. the mice were given a 10-day course o ... | 1977 | 212981 |
| a rational approach to the development of antiviral chemotherapy: alternative substrates of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) thymidine kinase (tk). | | 1977 | 212988 |
| mif-like activity in non-stimulated and virus infected cell cultures. | macrophage-migration inhibition factor (mif) is a lymphocyte-derived substance which plays an important role in cell mediated immunity. soluble factors containing mif-like activity and produced by non-stimulated and virus-infected non-lymphoid cell cultures have also been reported. in the present study, a mif-like factor was repeatedly detected in buffalo green monkey kidney cells infected with mumps and herpes simplex virus type 1 (hsv-1) indicating that this substance is reproducible and can b ... | 1978 | 213010 |