Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| synthesis and biological studies of 3-(beta-d-ribofuranosyl)-2,3,-dihydro-6h-1,3-oxazine-2,6-dione, a new pyrimidine nucleoside analog related to uridine. | reaction of the trimethylsilyl derivative of 2,3-dihydro-6h-1,3-oxazine-2,6-dione (2, "uracil anhydride") with protected 1-o-acetylribofuranoses in the presence of stannic chloride gave the corresponding block nucleosides. 3-(2,3-5-tri-o-2',2',2'-trichloroethoxycarbonyl-beta-d-ribofuranosyl)-2,3-dihydro-6h-1,3-oxazine-2,6-dione (4c) thus prepared from the protected sugar 3c, 1-o-acetyl-2,3,5-tri-o-(2,2,2-trichloroethoxycarbonyl)ribofuranose, gave, on removal of the protecting groups with zinc du ... | 1976 | 178870 |
| reactions of 2-acyloxyisobutyryl halides with nucleosides. 6. synthesis and biological evaluation of some 3'-acyl derivatives of 2,2'-anhydro-1-(beta-d-arabinofuranosyl)cytosine hydrochloride. | the reactions of cytidine with 22 different 2-o-acyloxyisobutyril chlorides lead to the isolation of the corresponding 2,2'-anhydro-1-(3'-o-acyl-beta-d-arabinofuranosyl)cytosine hydrochlorides 9. these compounds, which all show cytotoxicity against hela cells in tissue culture, have been examined for antiviral and antileukemic activity. activity against dna viruses (vaccinia and herpes) in tissue culture is maximal in compounds containing acyl groups with 8--12 carbon atoms. activity against l12 ... | 1976 | 178871 |
| reactions of 2-acyloxyisobutyryl halides with nucleosides. 7. synthesis and biological evaluation of some 2,2'-anhydro-1(3',5'-di-o-acyl-beta-d-arabinofuranosyl)cytosine hydrochlorides. | the direct acylation of 2,2'-anhydro-1(beta-d-arabinofuranosyl)cytosine hydrochloride (cycloc) with a homologous series of saturated and unsaturated acyl chlorides in dimethylacetamide has been investigated. such acylation reactions have made available a considerable number of 3',5'-diesters of cycloc that have been examined for biological activities. the compounds all show cytotoxicity against hela cells in tissue culture, and with the exception of the highly insoluble long-chain diesters (c16- ... | 1976 | 178872 |
| reactions of 2-acyloxyisobutyryl halides with nucleosides. synthesis and biological evaluation of some 3"-acyl and 3',5'-diacyl derivatives of 1-beta-d-arabinofuranosylcytosine. | previous papers in this series have described efficient syntheses of 3'-o-acyl and 3',5'-di-o-acyl and 3',5'-di-o-acyl derivatives of 2,2'-anhydro-1-(beta-d-arabinofuranosyl)cytosine hydrochloride (1,3). it has now been shown that the 2,2'-anhydro linkage in 1 and 3 can be selectively and efficiently cleaved by treatment with a mixture of pyridine and methanol giving the corresponding 3'-o-acyl derivatives of 1-beta-d-arabinofuranosylcytosine (2,4). the selective hydrolysis of the more soluble d ... | 1976 | 178873 |
| vaccinia virus meningitis in mice after intracerebral inoculation. | the pathogenesis of experimental vaccinia virus infection in weanling mice after intracerebral inoculation was followed with virological, histological, and immunohistological methods. high-dose inoculation, virus spread from brain to thoracic and abdominal viscera probably by an undetected early viremia. virus did rise to detectable levels in blood by day 5 and was found to be associated with the mononuclear cell fraction. by day 12, 30% of the animals had died and no further deaths occurred. ri ... | 1976 | 179952 |
| evaluation of 6-azauridine and 5-iododeoxyuridine in the treatment of experimental viral infections. | the potential antiviral activity of 6-azauridine and 5-iododeoxyuridine was evaluated in a coordinated study at five institutions. experimental models in five species, the mouse, rabbit, swine, cat, and ferret, were established with use of 10 viruses: herpesvirus hominis types 1 and 2, murine cytomegalovirus, vaccinia virus, shope fibroma virus, transmissible gastroenteritis virus, swine influenza virus, feline viral rhinotracheitis virus, feline panleukopenia virus, and ferret distemper virus. ... | 1976 | 180189 |
| effective antiviral prophylaxis and therapy by systemic application of human interferon in immunosuppressed monkeys. | rhesus monkeys treated with antilymphocytic globulin were infected with vaccinia virus or with medical lake macaque herpesvirus. at the sites of inoculation of vaccinia virus, animals not treated with interferon developed typical pustulous reactions and then smaller secondary lesions. human leukocyte interferon (5 x 10(5) international units per day) injected intramuscularly, either from one day before to three days after infection or for seven days beginning on the first day of primary reaction ... | 1976 | 180202 |
| the effect of tilorone hydrochloride on the growth of several animal viruses in tissue cultures. | tilorone hydrochloride, at a concentration of 10 mug/ml inhibits the growth of herpes simplex virus type 1 in bs-c-1 cells. the growth of vaccinia virus in bs-c-1 cells is partially inhibited; however, six viruses containing rna, including four members of the togavirus group grown in chick fibroblasts, are not affected by the drug. the inhibition of the growth of herpes virus by tilorone hydrochloride is greater when the multiplicity of infection is lower than 1 p.f.u/cell and when the drug is a ... | 1976 | 177724 |
| experimental viral infections of the inner ear. i. acute infections of the newborn hamster labyrinth. | acute viral infections of the inner ear were produced in neonatal hamsters. viruses were inoculated percutaneously through the temporal cartilage into the endolymphatic and perilymphatic spaces of the labyrinth or were inoculated intracerebrally to reach the perilymphatic spaces via the cochlear aqueduct. selective vulnerability of inner ear structures was demonstrated using a variety of viruses. influenza virus infected only the mesenchymal cells of the perilymphatic channels of the cochlea; mu ... | 1976 | 177808 |
| [ultrastructural study of the adsorption of lymphocytes on cells infected by vacinnia virus]. | adsorption of chicken lymphocytes to hep-2 cells infected with vaccinia virus was found to occur by two processes. in one, the lymphocytes were attached to the microvilli of the hep-2 cells, and the lymphocyte and host cell were separated at a distance of up to 0.6 micron. in the other, there was direct and close binding of the lymphocytes to the infected hep-2 cell. the lymphocyte and hep-2 cell were separated by an apparent gap approximately 10 to 20 nm in width. | 1976 | 139987 |
| [antiviral effect of acridine derivative c 283]. | 1976 | 176540 | |
| the effect of canaline on some events in vaccinia virus replication. | canaline, a pyridoxal phosphate antagonist, is shown to inhibit two distinct events in the replication of vaccinia virus in hela cells. initial events proceed in the presence of the inhibitor leading to the formation of dna-containing, cytoplasmic inclusions. however, further dna synthesis is required for the subsequent production of infectious progeny following the reversal of canaline inhibition by pyridoxal phosphate. inhibition of a separate, maturation event is shown by the delayed addition ... | 1976 | 128617 |
| role of early viral surface antigens in cellular immune response to vaccinia virus. | infection of mice with the vaccinia virus strain wr, elstree or dis, a coditional lethal mutant of vaccinia virus, resulted in the generation of vaccinia virus-specific sensitized cytolytic t lymphocytes (ctl). it could be shown by cross-reactivity between the three strains and by inhibition experiments with specific antisera that early vaccinia surface antigens are sufficient for the generation of specific ctl in vivo and for the lysis of infected target cells in vitro. | 1976 | 65141 |
| effect of atp and other nucleotides on the bleomycin-induced degradation of vaccinia virus dna. | 1976 | 56805 | |
| [cellular immunity in poxvirus infections. demonstration, significance and cellular cross reactivity with brain matter]. | its proof, significance, and the cellular cross reactivity to basic encephalitogenic protein of vaccinia-stimulated t-cells. vaccination against smallpox induces both, a cellular allergy and immunity, and the production of humoral antibodies. of greater importance for the defense of the organism against infections by orthopox viruses is the cellular immunity. new methods for the proof of cellular immunity and an in vitro-technique for its examination by challange with variola vera virus are desc ... | 1976 | 57915 |
| cell-mediated cytotoxicity against sendai-virus-infected cells. | after injection of sendai virus, a parainfluenza virus type i, mice generate cytotoxic lymphocytes which lyse specifically sendai-virus-infected target cells in vitro. their action is not inhibited by specific antibody in vitro. killer cell activity appears 4 days after infection, reaches a maximum on the 7th day and disappears on the 14th to 16th day. decrease of cytotoxic cell activity is correlated with an increase of haemagglutinating antibodies. the cytotoxic effector cell could be characte ... | 1976 | 60844 |
| the vaccinia virus haemagglutinin: enzyme sensitivity and some physiocochemical properties. | purified vaccina virus haemagglutinin (vha) was found to be sensitive to trypsin both with regard to haemagglutinating capacity and antigenic property. phospholipase c and lipase had no effect on the haemagglutination (ha). both the ha and the antigenic property thus depend on the integrity of the protein part. the loss of ha after treatment with trypsin may be due to breakdown of an essential protein or to secondary rearrangement of the whole structure, including the lipid part. cells infected ... | 1976 | 60873 |
| white rats as source of pox infection in carnivora of the family felidae. | 1976 | 11675 | |
| interferon-producing capacity of human tonsil cells and properties of interferon produced by these cells. | tonsils excised from human beings for chronic tonsillitis have been shown to be an accessible and sufficiently rich source of human lymphocytes. tonsil cells produced interferon as intensively as blood leukocytes and the properties of this interferon were similar to those of leukocyte interferon. the optimal conditions for interferon production by tonsil cells were established. | 1976 | 5865 |
| some biological and physical properties of molluscum contagiosum virus propagated in cell culture. | molluscum contagiosum virus propagated in fl cells of human amnion origin has a one-step growth cycle time of 12 to 14 h. the appearance and exponential increase of intracellular virus preceded the release of extracellular virus by approximately 2 h. demonstration of comparable titers of extracellular and intracellular virus at the end of the replication cycle indicated that a substantial amount of virus remained associated with cells exhibiting cytopathogenic changes. mean buoyant density value ... | 1976 | 8651 |
| early diagnosis of virus-caused vesicular rashes by immunofluorescence on skin biopsies. ii. poxvirus (vaccinia). | immunofluorescence (if) was used to demonstrate vaccinia virus antigen in frozen sections of skin biopsies from the site of revaccination in 42 individuals. the immunoglobulin (ig) of a rabbit anti-vaccinia serum and the ig of the pre-immune serum conjugated with fluorescein-isothiocyanate (fitc) was employed. 11/13 biopsies taken 1 day after vaccination were positive in the if test as were 13/13 biopsies taken 2 days and 14/16 biopsies taken 3 days after vaccination. even minute quantities of v ... | 1976 | 61622 |
| identification of antigens within the so-called vaccinia l-s complex by means of quantitative immunoelectrophoresis. | the so-called l-s antigen complex of vaccinia virus antigens was studied using gel diffusion and various immunoelectrophoresis methods. the low resolution power of the gel diffusion method and the inconsistency of the results obtained rendered this method unsuitable for identification of individual antigens and for comparative studies. the sensitivity and simplicity of counter current electrophoresis showed the superiority of this method for rapid diagnosis of pox-virus infections. simple rocket ... | 1976 | 61705 |
| a method for serological differentation of closely related poxviruses. | a two-step gel precipitation reaction with antiserum to vaccinia virus, based on sequential addition of two test viruses was elaborated for differentiation of closely related orthopoxviruses. the appearance of an additional precipitation line indicated differences in the antigenic structure of the viruses compared. differences in the antigenic structure of vaccinia, variola and monkey pox viruses as well as the antigenic identity with cow pox virus of poxviruses isolated from carnivora, elephant ... | 1976 | 61718 |
| the influence of negative staining on the infectivity of vaccinia virus. | 1976 | 61749 | |
| h-2 restriction of virus-specific cytotoxicity across the h-2 barrier. separate effector t-cell specificities are associated with self-h-2 and with the tolerated allogeneic h-2 in chimeras. | during infection with lymphocytic choriomeningitis or vaccinia virus, f1 irradiation chimeras reconstituted with bone marrow cells from or both parents generate cytotoxic t cells which can lyse targets across the h-2 barrier. however, activity of chimera t cells is h-2 restricted as shown by cold target competition experiments and selective restimulation of a secondary response in vitro; t cells of h-2k specificity which lyse tolerated infected h-2d target cells do not lyse infected h-2k or unre ... | 1976 | 62016 |
| inhibition of vaccinia virus replication in skin of tuberculin-sensitized animals challenged with ppd. | induction of a celayed-type hypersensitivity reaction in tuberculin-sensitized animals by tuberculin purified protein derivative (ppd) at the site of dermal vaccinia virus (vv) infection markedly accelerated elimination of vv and led to clinical recovery. viral titers were depressed by over 99.9% in the skin of animals given ppd as compared to animals given phosphate-buffered saline or nonspecific irritants. low concentrations of acid labile interferon were found in the skin of uninfected tuberc ... | 1976 | 62789 |
| shared determinants between virus-infected and trinitrophenyl-conjugated h-2-identical target cells detected in cell-mediated lympholysis. | infection of h-2-identical mice with either lymphocytic choriomeningitis (lcm) virus, vaccinia virus, or paramyxo (sendai) virus resulted in the generation of specifically sensitized cytotoxic t lymphocytes (ctl). ctl generated in vitro against 2,4,6-trinitrophenyl (tnp)-conjugated syngeneic stimulator cells were specifically cytotoxic for tnp-conjugated h-2k (d) region identical targets. both lcm and vaccinia-induced ctl, however, were found to be strongly cytotoxic towards tnp-conjugated, h-2k ... | 1976 | 63379 |
| [possibilities for the treatment of varicella-herpes zoster infections in juvenile leukemia]. | chicken pox and herpes zoster represent afraid complications in patients with malignant diseases which may take a noxious course. during the sequence of two chicken pox epidemias in 1973 in children with acute lymphoblastic leukaemias, the prevention and therapeutic possibilities were checked again. chicken pox or herpes zoster convalescent sera being compatible for blood groups and zoster-immunoglobulin were not available. at the time of the epidemia the first reports on the effect of cytarabin ... | 1976 | 64406 |
| electron microscopy of virus particles in ultrathin frozen sections. | a procedure with the use of polyethylene glycol (peg) 600 to support biological materials for ultracryotomy was developed. developing and mature virions were demonstrated by electron microscopy in negatively stained frozen sections of vaccinia virus-infected human diploid cells and of herpes simplex virus (hsv) type 1 infected primary rabbit kidney cells. approximate localisation of the virus particles in the cells was possible. | 1976 | 5872 |
| properties of dna ligase from uninfected and virus-infected hela cells. | hela cells contain a high m.w. form of dna ligase which can be completely converted to a low m.w. form. stokes radius, frictional ratio, sedimentation coefficient, molecular weight, ph dependence, and heat inactivation rate of the two forms have been studied. the major properties of the two forms of dna ligase in hela cells (in particular molecular weights and ph dependence) resemble those of the "dimer" and "monomer" structures described in cultured human cells (pedrali, g., spadari, s., ciarro ... | 1976 | 9621 |
| interference stimulated by tick-borne encephalitis virus: influence of divalent cations. | the effects of divalent cations (barium, cobalt, calcium, copper, magnesium, zinc and iron) on the interference of tick-borne encephalitis (te) virus with vaccinia virus in detroit-6 cell cultures were investigated. an increase in interference was shown in the presence of cobalt, zinc, copper, and magnesium. barium and iron displayed a marked inhibitory action. but an increase in interference was not parallel with an increased production of interferon. | 1976 | 9799 |
| molluscum contagiosum -- a defective poxvirus? | purified preparations of molluscum contagiosum virus contain a dna-dependent rna polymerase (ec 2.7.7.6) with similar but not identical properties to those of the enzyme found in vaccinia virions. the ultraviolet inactivation kinetics of the rna polymerase from both viruses were similar, displaying fast and slow components. ultraviolet irradiation destroyed the interfering capacities of molluscum and inactivated vaccinia virions, and the interferon-inducing capacity of molluscum virus slowly and ... | 1976 | 11275 |
| computer evaluation of antiviral activities of some thiosemicarbazones in experiments in vivo. | a new mathematical method based on 3-, 4- or 6-point parallel-line assays was applied for working out a computer programme used to evaluate the activity of some new isatin-beta-thiosemicarbazone derivatives against vaccinia virus, studied in vivo. | 1976 | 11668 |
| the effect of methylglyoxal bis(guanylhydrazone) on vaccinia virus replication. | 1976 | 999693 | |
| use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus. | a restriction endonuclease from haemophilus influenzae (hind iii) specifically cleaved vaccinia dna into 14 fragments. the molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). hind iii digestion of the dna from the wr and cv-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. the average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6). | 1976 | 1086369 |
| cells that express viral antigens but lack h-2 determinants are not lysed by immune thymus-derived lymphocytes but are lysed by other antiviral immune attack mechanisms. | murine f9 teratoma cells do not express major transplantation antigens detectable by either serologic or alloreactive assays of thymus-dependent lymphocytes (t cells). such cells can be infected with lymphocytic choriomeningitis virus or vaccinia virus, do express viral antigens on the cell surface, and can release infectious virus in amounts equivalent to those of other h-2 bearing murine cell lines. immunologic injury of virus-infected f9 cells occurs after the addition of specific antiviral a ... | 1976 | 1086476 |
| dna ligases of eukaryotes. | 1976 | 782919 | |
| further investigations on the mode of entry of vaccinia virus into cells. | earlier results indicating that vaccinia virus entered l cells by a process of direct fusion between the virus envelope and the plasma membrane of the cell have been confirmed and extended using immuno-ferritin conjugates to locate virus antigens on the host cell surface. after fusion, components of the virus envelope become rapidly dispersed in the plasma membrane. fusion has also been observed as the predominant mode of entry of vaccinia virus into hela cells. | 1976 | 798024 |
| modification of the 5'-terminals of mrnas by viral and cellular enzymes. | 1976 | 798242 | |
| further characterization of deoxyribonucleases from vaccinia virus. | 1977 | 14440 | |
| stability in drinking and surface water of nine virus species from different genera (author's transl). | the stability of nine viruses, aujeszky, sindbis, vesicular stomatitis, newcastle disease, vaccinia, fmd, hcc, reo and teschen virus in drinking and surface water was investigated comparatively at temperatures of 9 and 15 degrees c as well as the influence of water factors like seasonal difference in temperature, ph value, hardness and sort of water. the results can be summarized as follows: 1. at temperatures of 9 to 15 degrees c the majority of the viruses remained stabil in natural water for ... | 1977 | 14460 |
| 6-mfa, an antiviral agent from aspergillus ochraceus atcc 28706: influence of body weight and mineral oil administration of the antiviral activity in mice. | sera obtained from mice treated with 6-mfa, an antiviral agent from aspergillus ochraceus atcc 28706 showing high interferon activity, could be transferred to healthy animals to make them resistant to semliki forest virus (sfv) infection. body weight of mice directly influenced, within limits, the proportion of treated mice surviving challenge virus infection as well as interferon production. mineral oil (liquid paraffin, b. p.) administered prior to 6-mfa increased both the level of interferon ... | 1977 | 15439 |
| cellular proteases increased in paramyxovirus (sendai virus) carrier cells possibly responsible for enhanced formation of cowpox virus-specific cell surface antigen. | cowpox virus (cpv) growth and its s-ag (cell surface antigen) formation in hvj (sendai virus) carrier cells pre-treated with actinomycin d or puromycin were not affected as much as those in parent cells. these suggest the different cellular functions of carrier cells. the activity of carrier cell extracts causing a characteristic degradation of cpv reacted with them in vitro disappeared after the pre-incubation of extracts with hemoglobin or casein. measurements of cellular protease activities ... | 1977 | 15532 |
| a dna nicking-closing enzyme encapsidated in vaccinia virus: partial purification and properties. | vaccinia virus cores contain an activity which is able to relax both left-and right-handed superhelical dna. this virus-specific nicking closing enzyme has been highly purified and differs from the corresponding host enzyme in salt optimum, in sedimentation coefficient, and in polypeptide composition as determined on sodium dodecyl sulfate/polyacrylamide gels. the enzyme is probably newly synthesized after the cessation of host protein synthesis which follows virus infection. the most highly pur ... | 1977 | 17115 |
| purification of vaccinia virus by zonal centrifugation and analysis of viral protein composition. | vaccinia virus propagated in rotated cultures of rk13 cells was purified by sucrose density gradient zonal centrifugation. the purified virus was dissociated with sodium dodecyl sulfate end its protein composition analyzed by polyacrylamide gel electrophoresis. the protein composition of the virion envelope, isolated by the non-ionic detergent nonidet p-40, was also determined. | 1977 | 17277 |
| utilization of the mva-immunization schedule from the view point of the public health service. | the world-wide smallpox eradication program sponsored by the who has been successful. furthermore the legislation regarding smallpox vaccination in the federal republic of germany has been given a new form. the place of the previous mass-vaccination has been taken by vaccination of individuals. under this aspect safe procedures for vaccination are required. any vaccination used by public health service has to fulfill the following criteria: high protection against the disease without adverse sid ... | 1977 | 17567 |
| an analysis of factors influencing the isolation rate of herpes simplex virus. | attempts were made to improve the rate of isolation of herpes simplex virus (hsv) from clinical specimens by minimizing loss of virus infectivity during transportation and employing the most sensitive cells for isolation. basical analyses using standard strains of type 1 and type 2 hsv indicated that virus titer decrease was marked even at low temperatures in environments free of proteinous stabilizer such as normal serum or tissue extract, negating the generally held concept that hsv is stable ... | 1977 | 17816 |
| antiviral effect of tebrofen. | tebrofen inhibited the replication of western equine encephalomyelitis (wee) virus in multicycle experiments in chick embryo cell cell cultures. the compound did not inhibit the replication of vaccinia and newcastle disease viruses. | 1977 | 18926 |
| demonstration of vaccinia virus antigen in brains of postvaccinal encephalitis cases. | five lethal cases of postovaccinal encephalitis (pve) were examined in attempts to detect the viral antigen by the fluorescent antibody techniques and to isolate the infectious vaccinia virus. histologically, the brain tissues were characterized by meningoencephalitis, with perivascular infiltrations and inflammatous reactions mainly in leptomininx, chroid plexus and ependyma. by direct immunofluorescence staining, vaccinia virus antigen was found in the brain specimens of one case, but not in t ... | 1977 | 21318 |
| pathogenicity of a vaccine strain of vaccina virus in rabbits administered by intravenous route. | the pathogenicity of the lister (elstree) strain, a vaccinia virus, in rabbits after intravenous injection was studied by histopathological and immunofluorescent methods. inoculation of 1 x 10(7) pfu (pock-forming unit) virus into weanling and young adult rabbits caused severe emaciation and high mortality within 2 weeks. pathological findings were characterized by vesicular lesions along muco-cutaneous junction areas of the eyes, nose and mouth and by inflammatory changes in the brain, mainly i ... | 1977 | 24130 |
| regulation of herpesvirus thymidine kinase activity in lm(tk) cells transformed by ultraviolet light-irradiated herpes simplex virus. | 1977 | 65051 | |
| [suppression of experimental allergic encephalomyelitis with vaccinia-virus-induced anti-brain antibodies (author's transl)]. | 1977 | 68632 | |
| induction of virus-specific modifications recognized by cytotoxic t cells is not altered by prior substitution of target cells with trinitrophenol. | cytotoxic thymus-derived lymphocytes generated after interaction with trinitrophenyl (tnp)-substituted or virus-infected cells only lyse h-2 compatible target cells modified with the component used to immunize (tnp or virus). prior saturation of tnp-reactive sites inhibits neither the infectivity of influenza a viruses, nor the capacity of infected cells to develop antigenic changes recognized by influenza-immune t cells. the two antigens are distinct entities on the cell membrane and do not obv ... | 1977 | 69008 |
| in vitro immune responsiveness to vaccinea virus and hla. | because several lines of evidence suggest that hla products might have an important function in the immune response to infectious agents, we studied the possible relation between immune response to vaccinia virus and hla phenotype in 79 soldiers who received a primary vaccination. a low in vitro response to vaccinia virus was associated with hla-cw3 both in 49 subjects tested three to four weeks after vaccination (p less than 0.001) and in the remaining 30 subjects tested five to 11 weeks after ... | 1977 | 70749 |
| laboratory characteristics of poxviruses isolated from captive elephants in germany. | proxviruses isolated from captive elephants in germany have been characterized. although related to vaccinia and even more closely to cowpox virus, the separate identity of elephantpox virus was established by both biological and serological methods. elephantpox virus produces a-type inclusions in infected cells, as did cowpox, but had a lower ceiling temperature, was more heat resistant and affected rabbits differently. cross neutralization tests on absorbed sera indicated that elephantpox, cow ... | 1977 | 72786 |
| serological relatedness of monkeypox, variola, and vaccinia viruses. | closely related human and monkey orthopoxviruses were differentiated by serologic techniques. antiviral sera were tested by immunodiffusion for reactivity against six different viral antigens prepared from either infected cell cultures or infected chorioallantoic membranes (cams) of embryonated eggs. portions of each antiserum were separately absorbed with heterologous antigens from infected cams to remove common reactivity. the absorbed sera formed immunodiffusion precipitates with both types o ... | 1977 | 75947 |
| replication of vaccinia dna in mouse l cells. ii. in vitro dna synthesis in cytoplasmic extracts. | 1977 | 140514 | |
| [methods of expressive evidence of orthopoxviral infections tested on material from vaccinial skin lesions of rabbits (author's transl)]. | 1977 | 141340 | |
| emergence of foreign h-2-like cytotoxicity and transplantation targets on vaccinia and moloney virus-infected meth. a tumour cells. | using cytotoxic effector cells of different anti-h-2 specificities, new cell-mediated lympholysis targets, normally undetected on meth. a tumour cells, were shown after passage with vaccinia or moloney virus. the h-2d cml targets on meth. a cells recognized by b10.br anti-b10.d2 effector cells were presented only after simultaneous vaccinia virus passage, while passage with moloney virus caused the emergence of h-2b targets. small but significant killing of vaccinia virus-passaged meth.a was als ... | 1977 | 142300 |
| comparison of t cell-mediated immune responsiveness of nzb, (nzb x &nzw)f1 hybrid and other murine strains. | the age-dependent capacity of nzb and (nzb x nzw)f1 hybrid, balb/c, dba/2, c57bl/6 and c3h mice to generate t cell-mediated immune responses was assessed qualitatively and quantitatively by measuring the following effector functions: (a) the time course of alloreactive cytotoxic t-cell activity triggered in vitro was comparable for nz and other mouse strains; cell reactivity generated in vivo against el4 tumour cells was low in young (nzb x nzw)f1 mice and in dba/2 mice but was comparable for ol ... | 1977 | 142594 |
| carbocyclic arabinosyladenine, an adenosine deaminase resistant antiviral agent. | 1977 | 191615 | |
| comparative study of the antiviral activity of acyl derivatives of 2,2'-anhydro-1-beta-d-arabinofuranosylcytosine and other nucleosides against encephalitis in mice. | anti-deoxyribonucleic acid virus activities of 3'-o-acyl derivatives of 2,2'-anhydro-1-beta-d-arabinofuranosyl cytosine (cyclo-c) and 1-beta-d-arabinofuranosylcytosine (ara-c) were evaluated by using an in vivo test system in mice. among the derivatives tested, 3'-o-decanoyl cyclo-c hydrochloride was the most effective against herpes simplex virus-induced encephalitis in mice when the drug was administered directly into infected brains of mice (target-organ treatment). a comparative study of the ... | 1977 | 192142 |
| in vitro and in vivo inhibition of virus multiplicaton by microwave hyperthemia. | the effect of microwave hyperthermia (41 degrees and 43 degrees c) on virus multiplication have been explored in vitro (hsv-1 infected primary rabbit kidney cultures) and in vivo (mice infected with hsv-1 or vaccinia). in vitro the cells were inoculated with hsv-1 and heated to 41 degrees or 43 degrees c either before or after infection. virus yields were significantly decreased when the cells were exposed to hyperthemia within the first few hours after infection, while hyperthemia was without e ... | 1977 | 192183 |
| [prevention and therapy of viral diseases with special reference to interferon]. | human leukocyte interferon (hli) was used for treatment of human diploid fibroblasts before and after infection with vaccinia virus, herpes simplex virus type 1 (hsv 1), herpes simplex virus type 2 (hsv 2), and varicella zoster virus (vzv). vero cells were infected with medical lake macaque herpes virus (mlmv), and treated with hli in the same way. in all of these systems hli exhibited an antiviral effect when administered before infection, and this effect could be increased by additional hli tr ... | 1977 | 192653 |
| purification of vaccinia virus-induced thymidine kinase activity from [35s]methionine-labeled cells. | 1977 | 193257 | |
| antiviral activity of o'-methylated derivatives of adenine arabinoside. | 1977 | 193517 | |
| biogenesis of poxviruses: analysis of the morphogenetic sequence using a conditional lethal mutant defective in envelope self-assembly. | a conditional lethal, temperature-sensitive mutant of vaccinia, defective in assembly of the virus envelope and maturation, was characterized and shown to mimic exactly the aberrations produced by rifampicin. analyses of the infection at restrictive and permissive temperatures were conducted with electron microscopy, isotopic pulse-chase experiments in which polypeptides were separated by polyacrylamide slab gels, and assays of core enzymatic activities. the data collected by several approaches ... | 1977 | 194248 |
| molecular complexity of vaccinia dna and the presence of reiterated sequences in the genome. | 1977 | 194409 | |
| cytotoxic interactions of virus specific effector cells with virus infected targets of different cell type. | the action of sendai virus specific cytolytic t lumphocytes (ctl) against sendai virus infected macrophages was found to be h-2 restricted while sendai virus infected cell lines, including fibroblasts and tumour cells, were lysed across the h-2 barrier to some extent. the properties of the meth-a tumour cell, which was resistant to lysis by allogenic killer cells was investigated. sendai virus specific ctl failed to kill sendai virus infected meth-a cells but after vaccinia virus infection these ... | 1977 | 194984 |
| improved synthesis and in vitro antiviral activities of 5-cyanouridine and 5-cyano-2'-deoxyuridine. | in order to evaluate the influence of the cyano group on the antiviral activity of pyrimidine deoxyribonucleosides, a moderate yield, unified approach to the synthesis of both 5-cyanouridine and 5-cyano-2'-deoxyuridine was developed. thus, treatment of the appropriate acetylated 5-bromouracil nucleoside with nacn or kcn in me2so at 90-110 degrees c gave, after deblocking, 35-45% yields of the corresponding 5-cyanouracil nucleosides. 5-cyanouridine was devoid of significant activity against vacci ... | 1977 | 195058 |
| oncogenic transformation of rat embryo fibroblasts with photoinactivated herpes simplex virus: rapid in vitro cloning of transformed cells. | 1977 | 196034 | |
| possible antigenic sub-divisions within the variola/vaccinia subgroup of poxviruses. | 1977 | 196573 | |
| enhancement of virus growth produced by thiols and disulphides. | several thiols and disulphides have been found able both to shorten the latency phase and to increase the growth of several virus strains in cell cultures. | 1977 | 196894 |
| hindiii and sst i restriction sites mapped on rabbit poxvirus and vaccinia virus dna. | the dnas of two closely related orthopoxviruses, rabbit poxvirus (rpv) and vaccinia virus (vv), were mapped by overlapping-fragment analysis using restriction endonucleases hindiii and sst i. the exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. rpv and vv dnas showed identical restriction patterns in an internal region comprising approximately 60% of the genome. the size, by electrophoretical anal ... | 1977 | 197263 |
| radiation enhanced reactivation of nuclear replicating mammalian viruses. | 1977 | 197551 | |
| [cytochalasins in virologic research]. | 1977 | 197711 | |
| phosphorylation of ribosomes in adenovirus infection [proceedings]. | 1977 | 198265 | |
| the origins of vaccinia virus. | 1977 | 198484 | |
| structural polypeptides of several strains of orthopoxvirus. | 1977 | 198638 | |
| interferon-induced transfer of viral resistance between animal cells. | 1977 | 198670 | |
| mammalian nucleolytic enzymes. | 1977 | 198848 | |
| [research on substances with antiviral activity. vii. activity and lipophilic of property n-alkyl-2-chloro-3-formylindole thiosemicarbazone]. | thiosemicarbazones of n-alkyl-2-chloroindole-3-carboxaldehydes were synthesized and investigated for antiviral activity against rna virus (parainfluenza type 3, ha-i/cr-8 stock) and dna virus (vaccinia virus, hid stock). the effect of lipophilicity on the activity against vaccinia virus was considered. relative lipophilicities were measured by a reversed phase thin-layer chromatographic technique. the most active compound n-isopropyl-2-chloroindole-3-carboxaldehyde thiosemicarbazone appeared com ... | 1977 | 200469 |
| synthesis of n6- or 8-substituted 9-(beta-d-arabinofuranosyl)-adenines and their antiviral activities against herpes simplex and vaccinia viruses. | 1977 | 201386 | |
| differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay. | poxvirus antisera adsorbed with "homologous" and "heterologous" poxvirus-infected chorioallantoic membranes (cam) were differentiated by solid-phase radioimmunoassay (ria). mixtures of the antiserum dilutions and infected cam were added directly (without centrifugation) to poxvirus-infected cam antigens affixed to wells of microtitration plates. the affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen-antibody complexes were removed by washing. the results showed ... | 1977 | 201390 |
| 1,2,4-triazole amino nucleosides. 1-beta-d-3'-amino-3'-deoxyribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides. | the synthesis of 1-beta-d-3'-amino-3'-deoxyribofuranosyl-1,2,4-triazole-3-carboxamide--the 3'-amino analogue of ribavirin--and five related nucleoside analogues is described. each analogue exhibited ld50 concentrations greater than 100 microgram/ml against p-388 mouse lymphoid leukemia cells in tissue culture. antiviral testing indicated that none of the compounds exhibited significant activity. | 1977 | 201758 |
| poxvirus hosts and reservoirs. brief review. | 1977 | 202224 | |
| rescue of host-dependent conditional lethal mutants of vaccinia and rabbitpox viruses by yaba virus. brief report. | 1977 | 202231 | |
| virus infection of murine teratocarcinoma stem cell lines. | 1977 | 202395 | |
| a new approach in specific, active immunotherapy. | a vaccinia virus-lysed autochthonous tumor cell vaccine (vaccinia oncolysate) is introduced as a new specific, active immunotherapeutic agent against human cancer. mouse experiments showed the vaccine to be a safe and potent immune mechanism stimulator. human experimentation was undertaken in the knowledge of relative safety of the components of the vaccine, i.e. vaccinia vaccine and lysed, autochthonous tumor cells. vaccine-treated patients had advanced metastatic cancer but reacted to one or m ... | 1977 | 189895 |
| [determination of autohemolysin formation using the plaque test. demonstration of autoimmune phenomena following vaccinations and infections]. | a simple and sensitive modification of the plaque assay for the evaluation of autohaemolysin-producing cells (apc) in the peripheral blood is described: very small chambers, (volume less than or equal to 0.03 cc) made out of glass slides (75 x 25 mm), are filled with a diluted (1:7) blood cell suspension. after incubation for 26 hours the haemolytic plaques produced by apc in the thin monolayer of blood cells can be counted under the microcope at a magnification up to 320-fold. the number of apc ... | 1977 | 190096 |
| quantitative immunoelectrophoretic studies of the l and s antigens of vaccinia virus. | the l (10s) and s (7s) antigens of vaccinia virus have been characterized by their sensitivity to heat, to proteolytic enzymes and to periodate. the l antigen was heat labile and inactivated by proteolytic enzymes and periodate. treatment with trypsin for 1 min indicated that the s antigen was built up of at least two protein subunits of about 3s and 5s. one of the subunits was highly sensitive to heat and to proteolytic enzymes. on further treatment with trypsin the other subunit was split in t ... | 1977 | 203163 |
| factors concerning the immunity to poxvirus infection. | two kinds of early antigens were shown in vaccinia-infected cells, one heat (56 degrees, 30 min)-stable (es antigen) and the other heat-labile (el antigen). both antigens do not induce circulating neutralizing antibody in rabbits, but resistance to intradermal inoculation of active virus was observed when animals were immunized with either antigen in combination with freund's complete adjuvant. vaccinia-specific haemagglutinin (vha) associated with infected cell membrane was purified and its ant ... | 1977 | 203217 |
| effect of vaccinia virus and herpes simplex viruses type 1 and 2, on the chromosomal system of human lymphocytes, in cultures in vitro. | 1977 | 203354 | |
| effect of selective complement deficiency on the rate of neutralization of enveloped viruses by human sera. | the capacity of human sera genetically deficient in selective complement (c) components to enhance neutralization of enveloped viruses was examined by kinetic plaque reduction assays. vaccinia virus, a dna virus, and vesicular stomatitis virus (vsv), an rna virus, were studied. exogenous rabbit: or human antibody to vaccinia virus, and guinea pig or human antibody to vsv were provided in limiting, c-dependent concentrations. igg antibodies predominated in most of the antisera employed. c5-defici ... | 1977 | 187701 |
| the virion and soluble antigen proteins of variola, monkeypox, and vaccinia viruses. | the structural proteins in purified preparations of variola, monkeypox, and vaccinia viruses were separated and compared by using a high resolution sds-polyacrylamide gel electrophoresis system. about 30 proteins were resolved for each virus by autoradiography of longitudinally-sliced gel rods. although the autoradioelectropherograms of each virus were similar, it was possible to differentiate them by their unique protein pattern in the 30,000 to 40,000 molecular weight region of the gels. a sin ... | 1977 | 205632 |
| comparison of antiviral and antitumor activity of activated macrophages. | 1977 | 191199 | |
| changes in whole blood and serum components during francisella tularensis and rabbit pox infections of rabbits. | rabbits infected with virulent francisella tularensis strain schu s4 or rabbit pox virus (utrecht strain) showed significant early changes in serum levels of trace metals, neutral fat and alkaline phosphatase activity. with f. tularensis infections a marked early leukopenia and a decrease in serum amino acids were also observed; the effect on amino acid levels was less pronounced in rabbit pox infections. in both diseases these changes preceded the appearance of acute phase globulins in the seru ... | 1977 | 204323 |
| [potential virostatics. 1. quinoxalines]. | 1977 | 203954 | |
| [combined action of dna-synthesis inhibitors on the reproduction of dna viruses]. | 1977 | 204162 | |
| high voltage electron microscopic observations of some poxviruses. | 1977 | 214503 |