anti-inflammatory drug actions on allergic responses in guinea-pig skin.five non-steroidal anti-inflammatory drugs (indomethacin, naproxen, meclofenamic acid, feprazone and phenylbutazone: nsaids) and three glucocorticosteroids (dexamethasone, hydrocortisone and prednisolone) have been tested as local inhibitors of increased vascular permeability in guinea-pig skin. lesions were induced by histamine or by antigen to evoke type i (passive cutaneous anaphylaxis), type iii (reverse passive arthus) and type iv (delayed hypersensitivity) allergic reactions. nsaids and gl ...197831078
blockade by n-methylhydroxylamine of activation of guanylate cyclase and elevations of guanosine 3',5'-monophosphate levels in nervous tissues.hydroxylamine and n-methylhydroxylamine prevented the activation of soluble guanylate cyclase by the endogenous activator as well as by nitroso compounds such as n-methyl-n'-nitro-n-nitrosoguanidine or nitroprusside, while other derivaties of hydroxylamine were ineffective. hydroxylamine and n-methylhydroxylamine did not alter the basal guanylate cyclase activity of purified enzyme preparations. kinetics analysis indicated that n-methylhydroxylamine competes with n-methyl-n'-nitro-n-nitrosoguani ...197831192
the effects of alpha- and beta-adrenoceptor agonists on inflammatory exudation in rabbit and guinea-pig skin [proceedings]. 197831224
stimulation of arachidonic acid metabolism by human slow-reacting substances.human slow-reacting substance of anaphylaxis (srs-a) and calcium ionophore-induced human srs released prostaglandin-like substances and rabbit aorta contracting substance (rcs) from guinea-pig lungs. this effect was abolished by incubation of srs-a and srs with arylsulphatase or pretreatment of the lungs with indomethacin. human srs-a and srs therefore resembled guinea-pig srs-a in stimulating arachidonic acid metabolism. these results provide further evidence for a similar (or identical) nature ...197831288
dopamine antagonistic effects of a series of analogues of oxiperomide and spiroxatrine measured behaviourally in the rodent.the activity spectra of oxiperomide, spiroxatrine and analogues were determined in two experimental models of abnormal peri-oral movements (induced by intrastriatal dopamine and subcutaneously administered 2-(nn-dipropyl)amino-5,6-dihydroxy-1,2,3,4-tetrahydronaphthalene (nn-dipr-5,6-diohatn) in the guinea-pig), and in a stereotypy test (induced by subcutaneous apomorphine in the guinea-pig); the ability of the test compounds to induce catalepsy or catatonia in the rat was also determined. the pa ...197831432
the effects of a hydroxyl group on some chemical and biological properties of n-pentyl ammonium salts.the effects of a hydroxyl group on the activity and affinity of compounds related to n-pentyltrimethylammonoum have been studied on the guinea-pig ileum, frog rectus, acetylcholinesterase and on partitioning (rm) and size (phiov, vm). the hydroxyl group lowered affinity in all tests, confirming the importance of hydrophobic forces in binding to receptors. activity on the ileum was lowered appreciably but on the rectus only slightly. the effects on rm did not indicate any interactions between hyd ...197831434
the influence of neuroleptics on amphetamine metabolism in the rat and guinea-pig. 197831438
adrenergic and 'non-adrenergic' components in the contractile response of the vas deferens to a single indirect stimulus.1. the mechanical response of the longitudinal smooth muscle of the rat vas deferens to stimulation of its motor nerves by a single pulse has been examined. the motor nerves were stimulated in vivo via the spinal outflows in the pithed rat or in vitro by field stimulation. 2. the contraction in the whole vas consisted of two components, an initial, rapid, brief contraction reaching a maximum at 300 msec and a second, slower and more prolonged contraction reaching its maximum at 600 msec. when th ...197831466
synthesis and beta-adrenoceptor activity of the erythro and threo isomers of substituted alpha-hydroxytrimetoquinol.the synthesis and pharmacological activity of erythro and threo isomers of 1-(3',4',5'-trimethoxy-alpha-hydroxy-benzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, 2 and 3, are reported. the structural assignments of 2 and 3 are based upon nmr spectra of the 6,7-dibenzyl precursors, 6 and 10, and of the synthetic derivatives of 13alpha- and 13beta-hydroxy-2,3-(dibenzyloxy)-9,10,11-trimethoxytetrahydroprotoberberine, 8 and 12, respectively. the erythro isomer 2 was a more potent beta-adrenocept ...197831479
the site of action of capsaicin on the guinea-pig isolated ileum. 197831571
factors influencing the release of acetylcholine from the myenteric plexus of the ileum of the guinea-pig and rabbit.1 the effects of electrical stimulation, changes in external ion concentrations and various drugs on acetylcholine release from the myenteric plexus were measured by bioassay in the presence of physostigmine and by recording the responses of the longitudinal muscle. in preparations from the guinea-pig, the acetylcholine output per pulse increased with decreasing frequency of stimulation and reached its maximum at a frequency of 0.017 hz (1/min) and thus ensured that the output per unit of time w ...197831960
irreversible beta-adrenoceptor blockade of atrial rate and tension responses.the competitive reversible beta-adrenoceptor antagonist activity of ro 03-5255 [1-(5-acetylaminobenzfuran-2-yl)-2-isopropylaminoethanol] upon isoprenaline-induced increases of the rate and tension of guinea-pig isolated atria is described. the chlorinated derivative [ro 03-7894; 1-[5-chloracetylaminobenzfuran-2-yl)-2-isopropyl-aminoethanol] in contrast exhibited concentration-dependent non-competitive irreversible blocking activity as measured by depression of the maximum responses which were no ...197832050
blockade by wb 4101 of alpha-adrenoceptors in the rat vas deferens and guinea-pig taenia caeci.the effectiveness of wb 4101, a recently described alpha-adrenoceptor antagonist, in blocking an excitatory and two inhibitory responses to alpha-receptor activation was studied. one of the inhibitory responses was the reduction by the selective alpha-agonist amidephrine of carbachol contractures of isolated guinea-pig taenia caeci. wb 4101 antagonised this inhibition with a schild plot slope of 0.99 and a pa2 of 8.9. the same pa2 value was obtained for blockade of the contractile effect of amid ...197832052
a difference in effects of physiological ca2+ concentrations on activity of guanylate cyclase preparations obtained from the taenia caecum of guinea pig and from the longitudinal muscle of rat duodenum.a cholinergic stimulant, butyltrimethylammonium bromide and serotonin increased the tissue levels of cyclic gmp in the taenia caecum of guinea pig but not those in the longitudinal muscle of rat duodenum. on the other hand, physiological ca2+ concentrations enhanced the activity of a guanylate cyclase preparation obtained from the taenia caecum of guinea pig, while guanylate cyclase in the longitudinal muscle of rat duodenum was not influenced by ca2+. the difference in the effects of the smooth ...197832054
antagonism of central histamine h1 receptors by antipsychotic drugs.the activity of 8 widely antipsychotic drugs as antagonists of central histamine h1 receptors was determined from the inhibition of the binding of 3h-mepyramine to a membrane fraction from guinea-pig brain. the phenothiazines examined, clorpromazine, fluphenazine, thioridazine and trifluoperazine, were all potent h1 antagonists. both alpha- and beta-flupenthixol were potent inhibitors, but butaclamol, although less potent, showed stereospecificity. haloperidol and spiperone were markedly weaker ...197832056
the effect of pge1 on peristalsis and on perivascular nerve inhibition of peristaltic activity in guinea-pig isolated ileum.the effect of pge1 on peristalsis of guinea-pig isolated ileum was examined using a modified trendelenburg method to evoke and record peristaltic activity. pge1 (14 nm, 0.11 microm and 0.56 microm) increased peristaltic activity of both longitudinal and circular muscle, mainly by increasing the amplitude of contraction. preparations of ileum subjected to a 'minimal' peristaltic stimulus were more sensitive to the effects of pge1 than were preparations subjected to a 'just-maximal' peristaltic st ...197832240
effects of the antiemetic drug domperidone on guinea-pig isolated gastrointestinal tissue [proceedings]. 197832289
uptake of amphetamine by guinea-pig lung in vitro [proceedings]. 197832312
effects of 5-hydroxykynurenamine, a metabolite of 5-hydroxytryptamine, on guinea-pig isolated trachea. 197932367
evidence for the existence of another type of histamine h2-receptor in guinea-pig ileum. 197932369
metoclopramide reverses inhibitions of electrically-induced contractions of guinea-pig isolated ileum by anti-inflammatory drugs. 197932372
the isolation of carbon dioxide-requiring (carboxyphillic) type 19 pneumococcus (streptococcus pneumoniae) from diseased guinea-pigs.type 19 pneumococcus was isolated only in anaerobic cultures from tissue fluids of guinea-pigs suffering from exudative pericarditis and pleuropneumonia. the organism was pathogenic to mice even after 40 subcultures. further study showed it to be carboxyphilic rather than anaerobic. the necessity of specifying various atmospheric conditions in the diagnostic bacteriological isolation routine is emphasized.197832435
pharmacological characterization of histamine receptors mediating the stimulation of cyclic amp accumulation in slices from guinea-pig hippocampus. 197832479
tyrosine hydroxylase and dopa decarboxylase activities in the guinea-pig uterus: further evidence for functional adrenergic denervation in association with pregnancy. 197932500
differentiation of the roles of histamine h1- and h2-receptors in the mediation of the effects of histamine in the isolated working heart of the guinea-pig.1 differentiation of the roles of histamine h1- and h2-receptors in the mediation of the effects of histamine on the isolated working heart of the guinea-pig was achieved through the use of histamine and selective histamine receptor agonists and antagonists. 2 histamine over the dose range 10(-9) mol to 10(-6) mol produced dose-related increases in sinus rate, left intraventricular pressure (lvp)max, lvdp/dtmax, coronary flow, aortic flow, total cardiac output and external pressure-volume work. ...197932943
lack of release of prostaglandins from isolated perfused lungs during pulmonary hypertension and oedema.1 the effects on pulmonary prostaglandin synthesis of pulmonary hypertension and oedema have been studied. 2 seventeen isolated lungs of rabbit, cat and guinea-pig were perfused with plasma, whole blood or krebs ringer solution in a recirculating system. 3 the venous effluent from the lungs superfused (10 ml/min) a series of smooth muscle tissues sensitive to prostaglandins and thromboxane a2; these were: rat stomach strip, rat colon, chick rectum and rabbit aorta. 4 left atrial pressure was inc ...197932945
the sites of action of 5-hydroxytryptamine in nerve-muscle preparations from the guinea-pig small intestine and colon.1 the sites of action of 5-hydroxytryptamine (5-ht) were examined in isolated segments of guinea-pig intestine. mechanical records were taken from the longitudinal muscle of the ileum and proximal colon and from the circular muscle of the ileum and distal colon.2 in order to examine direct actions of 5-ht, nerve-mediated responses were blocked with tetrodotoxin (0.2 mug/ml). there was a gradient in the responsiveness of the longitudinal muscle of the ileum; in the proximal ileum it was usually u ...197932947
stereochemical aspects of amine substrate attachment to acyl intermediates of transglutaminases. human blood plasma enzyme (activated coagulation factor xiii) and guinea pig liver enzyme. 197933172
possible differences in alpha-adrenoceptors in rat, guinea-pig and rabbit spleen. 197933240
substance p causes direct depolarisation of neurones of guinea pig interpeduncular nucleus in vitro. 197933338
kinetic studies in isolated organs: tools to design analgesic peptides and to analyze their receptor effects.the opioid activities of peptide and non-peptide narcotics were studied in longitudinal muscle strip of guinea pig ileum (gpi) and in mouse vas deferens (mvd). the comparison of agonist activities of peptides found in gpi and mvd offered the opportunity to predict the presence but not the magnitude of potential analgesic activity. the kinetics of the antagonism between naltrexone and different types of agonists were also determined in these systems. using c-6 epimers of naltrexone, it was found ...197933396
[the reaction of lung mast cells to acute barbiturate and bromureide poisoning (author's transl)].guinea pigs were poisoned by large oral doses of hypnotics in order to study the effect of bromureides on the mast cell contents of the lungs in comparison to the influence of barbiturate intoxication. the dose of the hypnotics chosen was thus, that all animals died within the first three hours after the beginning of feeding. then the degranulation of mast cells with decrease of the mast cell number in the lung tissue was merely small in the barbiturate poisoned animals, but very extensive in th ...197833510
the influence of aging on the myosin type of the rabbit soleus and longissimus dorsi muscles.the influence of aging on the myosin type, and on the fibre composition of both the slowly contracting ("red") m. soleus and the fast ("white") m. longissimus dorsi was examined in the rabbit. for myosin characterization isolated myofibrils were electrophoresed on sds-polyacrylamide gels, and the fibre pattern within the respective muscles was analyzed with an immunocytochemical method. antisera against either fast or slow rabbit myosin were collected from guinea pigs after longterm immunization ...197833562
[mechanism of action of 8-n-phenethyl-1-oxa-2-oxo-3,8-diaza-(4,5)-spirodecane hydrochloride, a bronchodilator agent, on the isolated trachea of the guinea pig]. 197833684
a study of carbachol-atropine interaction on intestinal smooth muscle vesicles, using a fluorescent probe.plasma membrane vesicles were prepared from guinea pig ileum longitudinal muscle. the vesicles were characterized by electron microscopy and analysis of lipid and protein content. they were shown to be free of gross contamination from actomyosin, sarcoplasmic reticulum, and mitochondria. 8-anilino-1-naphthalene sulphonic acid (ans) binding characteristics were similar to those found in other membranes. both carbachol and atropine increased the fluorescence of ans bound to this membrane, the maxi ...197833755
isolated lung strips of guinea pigs: responses to beta-adrenergic agonists and antagonists.isolated lung strips of guinea pigs were examined as an in vitro model for assessing the direct effect of beta-adrenergic drugs at the level of peripheral airways. changes in intrinsic tone of thin strips of lung parenchyma were measured with an isometric force transducer. isoproterenol, a nonselective beta-adrenergic agonist, and several beta-adrenergic agonists, soterenol, salbutamol, metaproterenol and ritodrine elicited a dose-related relaxation of lung strip. responses to isoproterenol were ...197933813
[alpha- and beta-adrenoceptor blocking actions of labetalol and effects on the myocardial function, coronary circulation and myocardial energy metabolism thereof (author's transl)].in the isolated atria, trachea and aorta of the guinea pig and in the heart-lung preparation (hlp) of the dog, labetalol was 3.6 approximately 5.9 times less potent than phentolamine in blocking alpha-adrenoceptors, 1.4 approximately 3.6 times less potent as beta 1-blocker and 6 times less potent as beta 2-blocker than propranolol. beta 1 and beta 2-blocking actions were 12 approximately 28 and 7 times more potent than alpha-blocking action, respectively. as regards beta 1-blockade, attenuation ...197833880
synthesis and preliminary biological studies of 4- and 5-[2-hydroxy-3-(isopropylamino)propoxy]benzimidazoles: selective beta2 adrenergic blocking agents.benzimidazoles carrying the 2-hydroxy-3-(isopropylamino)propoxy side chain at either the c-4 or c-5 ring positions were synthesized and investigated for beta-adrenergic blocking activity. both compounds demonstrated beta2 selectivity when evaluated in guinea pig atrial and tracheal preparations. the c-4 isomer was 17 times more selective toward tracheal tissue, and its overall potency was roughly comparable to that of propranolol. beta2 selectivity of the c-5 isomer was minimal, with a potency a ...197934040
oxidative metabolism of chicken polymorphonuclear leucocytes during phagocytosis.the oxidative response to phagocytosis by chicken polymorphonuclear leucocytes was investigated as compared to guinea pig polymorphonuclear leucocytes. the polymorphs from both species respond to phagocytosis with an increased oxygen consumption, an increased generation of o2 and h2o2, and an increased oxidation of glucose through the hexose monophosphate shunt. the rate of oxygen consumption, and generation of o2- and h2o2 by phagocytosing chicken polymorphonuclear leucocytes is considerably lo ...197834093
the effect of muscarine on cholinoceptive neurones subserving the peristaltic reflex of guinea-pig isolated ileum. 197834117
absence of tyrosine hydroxylase activity and dopamine beta-hydroxylase immunoreactivity in intrinsic nerves of the guinea-pig ileum. 197934125
cardiac muscarinic blocking and atropinic blocking effects of a tetramine disulfide and alpha-adrenoceptor blocking activity.since many alpha-adrenoceptor blocking drugs were known to have muscarinic blocking activity, bhc, a newly synthesized tetramine disulfide with alpha-adrenoceptor blocking activity, and two of its analogs were tested for muscarinic blocking activity in the isolated guinea pig atrium and ileum. bhc had muscarinic blocking activity. its potency was higher in the atrium than in the ileum. the cardiac muscarinic blocking potency of the analog that contained carbon atoms instead of sulfur atoms was s ...197934474
adenylate cyclase of guinea pig skeletal muscle sarcolemma: comparison of the properties of the enzyme with mg2+ and mn2+ as divalent cation cofactors. 197934539
insulin receptors in cultured mouse retinal cells.the binding of 125i-insulin to uncloned and cloned cultures of mouse retinal cells has been investigated. at 15 degrees c, binding of the hormone reached a steady state by 60 min, while at 37 degrees c equilibrium was reached earlier but at a lower level than at 15 degrees c. porcine insulin, porcine proinsulin and guinea pig insulin displaced labelled insulin in proportion to their known biological potency. a sharp ph dependence of the hormone binding was observed with an optimum at ph 7.8. the ...197934549
percutaneous absorption: in vivo experiments.the percutaneous absorption of esters of nicotinic acid has been studied in vivo in man. the time for erythema to be produced has been measured both when the ester is applied continuously and in 'pulse' experiments when the ester is removed before the erythema develops. the results show that the erythema is produced long before steady state diffusion across the epidermis is established and the penetration of methyl nicotinate is characterized by d/l2 = 2.3 x 10(-4)s-1 where d is the diffusion co ...197934686
pentobarbitone and skeletal muscle contractions: on the interaction with the effect elicited by the beta-adrenoceptor agonist, terbutaline.the soleus, a slow-contracting muscle, and the extensor digitorum longus (edl), a fast-contracting muscle from guinea-pig were prepared for isometric recording in vitro. subtetanic contractions were evoked by transmural field-stimulation. pentobarbitone increased the force of contraction in both muscles. in the soleus it shifted the stimulation frequency-response curve to the left. terbutaline caused a decrease in the force of subtetanic contractions of the soleus, an effect which was dependent ...197934691
the inhibitory effects of sex steroid hormones on electrically-induced contractions of the guinea-pig isolated ileum. 197934699
[the influence of hypnotics on the development of morphological and biochemical wound reaction (author's transl)].the influence of hypnotics (barbital and carbromal) on the development of the early wound reactions in mechanically injured skin of guinea pigs was investigated: 1) the cellular reactions in incised wounds were retarded after moderate intoxication by hypnotics (300 mg/kg barbital; 3 g/kg carbromal). the histomorphological changes in wounds were inhibited by carbromal twice as much as by barbital. the hitherto published investigations had not shown any retardation of the early cellular inflammati ...197934946
effects of beta-adrenoceptor agonists on airway smooth muscle and on slow-contracting skeletal muscle: in vitro and in vivo results compared.ten beta-adrenoceptor agonists were examined with respect to a) relaxation of pilocarpine-contracted trachea and depression of contractions of the soleus muscle of the guinea-pig in vitro and b) counteraction of histamine-induced bronchospasm and depression of contractions of the soleus muscle of the cat in vivo. there was a close correlation between the results obtained in vitro and results obtained on the corresponding tissues in vivo in spite of the different species used. a close correlation ...197934976
intrabiliary pressure changes produced by narcotic drugs and inhalation anesthetics in guinea pigs.the effects of narcotic agents and two inhalation anesthetics on intrabiliary pressure (ibp) were measured before and after morphine (0.2 mg/kg), meperidine (2 mg/kg), fentanyl (0.002 mg/kg), or pentazocine (1 mg/kg) given intramuscularly to guinea pigs, and after halothane (0.5, 1.0, 1.5, or 2.0 mac) or enflurane (same range of mac) administered by inhalation. all narcotics except pentazocine significantly increase ibp, the increases ranging from 85.7% for meperidine to 143.4% for fentanyl. pen ...197935040
effects of volatile anesthetics on directly and indirectly stimulated skeletal muscle.isolated guinea pig nerve-lumbrical muscle preparations were exposed to halothane, methoxyflurane, isoflurane, enflurane, fluroxene, and diethyl ether. the temporal courses of the effects on indirectly and directly elicited twitch responses were determined over a range of concentrations for each agent. when the anesthetics were compared at concentrations equivalent in terms of minimum alveolar concentration (mac), a spectrum was observed in which halothane, methoxyflurane and isoflurane depresse ...197935042
specificity of guinea pig liver transglutaminase for amine substrates.the amine specificity of guinea pig liver transglutaminase, a model enzyme for endo-gamma-glutamine:epsilon-lysin transferases, was explored with the aid of synthetic substrates of high apparent affinities. as exemplified by dansyl- (5-dimethylamino-1-naphthalenesulfonyl), (2,4-dinitrobenzenesulfonyl)-, and (2,4,6-triisopropylbenzenesulfonyl)-cadaverines--each of which showed affinities of approximately 4 x 10(7) m-1--the best amine substrates carried a large hydrophobic substituent attached to ...197935225
the effect of beta-adrenoceptor and ca2+ antagonist drugs on the hypoxia-induced increased in resting tension.using isolated, langendorff-perfused, electrically-paced guinea-pig hearts, we have investigated the rise in resting tension that occurs when mammalian heart muscle becomes hypoxic. substrate-depletion, tachycardia, hyperthyroidism, and inotropic interventions (ouabain, isoprenaline, and beta-receptor antagonists at concentrations which increase inotropic state) enhanced the rate of development of this increase in resting tension. 3.86 mumol.litre-1 propranolol, 0.22 to 2.20 mumol.litre-1 verapa ...197835282
maturational changes in adrenal xenobiotic metabolism in male and female guinea young (25-day-old) guinea pigs, adrenal and hepatic benzo[a]pyrene (bp) hydroxylase activities were similar but the rates of ethylmorphine (em) demethylation were greater in adrenals than liver. between 25 and 50 days of age no sex differences in adrenal or hepatic enzyme activities were demonstrable. the rates of adrenal bp and em metabolism increased with age in guinea pigs of both sexes; activities reached significantly higher levels in males than females. in contrast, hepatic metabolism o ...197935328
cow red blood cells. ii. stimulation of bovine red cell glycolysis by plasma.cow red cell glycolysis, which can be stimulated by a variety of purines and pyrimidines, was also found to be elevated by its own plasma. dialyzed or charcoal-treated plasma could no longer stimulate glycolysis, suggesting that the stimulating factors may be purines or pyrimidines. determination of purines or pyrimidines in plasma revealed the presence of xanthine (0.31 mum), hypoxanthine (0.60 mum), and adenosine (0.05 mum), as well as unknown compounds. a physiologic level of hypoxanthine, wi ...197935980
humoral regulation of vascular resistance after 30 days of pulmonary artery an earlier study of guinea pigs with constriction of the pulmonary artery (pa) for 30 days, hindquarters' vascular resistance was maintained primarily by humoral mechanisms. in the present study, we investigated the contribution of circulating catecholamines, angiotensin ii, and other constrictor stimuli to hindquarters' vascular resistance by observing vasodilator responses to specific competitive antagonists. pressure-flow curves indicated vascular resistances in isolated, perfused, sympath ...197935987
a fluorimetric assay for angiotensin-i converting enzyme in human serum.a fluorimetric method is described for a simple, sensitive and reproducible assay for angiotensin-i converting enzyme in human and guinea pig sera. the very weak fluorescent substrate o-aminobenzoylglycyl-p-nitro-l-phenylalanyl-l-proline is enzymatically hydrolyzed, producing the highly fluorescent o-aminobenzoylglycine that is quantitatively determined by spectrofluorimetry. dependence of activity on substrate concentration, amount of serum, time of incubation and ph were investigated. the km v ...197936243
luminal alkalinization by guinea-pig cecum in vitro, an electro-neutral process.guinea-pig cecum was found to alkalinize its mucosal media in vitro at a chemical equivalents rate greater than the short circuit current (isc). alkalinization was inhibited by conditions which did not affect isc and by low mucosal na suggesting an electro--neutral process, dependent on na.197936288
effects of adrenergic and cholinergic agonists on adenylate and guanylate cyclase activity of isolated guinea-pig seminal vesicle epithelium.isolated seminal vesicle epithelium of the guinea-pig contained increased amounts of camp and cgmp after treatment with pge1 and carbachol, respectively. adrenergic agents had no influence. possible physiological implications of these results are discussed.197936292
characterization of receptors on postganglionic cholinergic neurons in the guinea-pig isolated ileum.dopamine, apomorphine, noradrenaline and isoprenaline reduced the response of the isolated guinea-pig ileum to exogenous acetylcholine by a maximum of 40%. propranolol reversed this inhibition whilst phentolamine and pimozide were ineffective, suggesting that the drugs were acting on a post-synaptic beta-adrenoceptor. the same agonists were more effective as inhibitors of the response to transmural electrical stimulation of the ileum, lower doses producing almost complete inhibition. this inhibi ...197936459
effect of naloxone on the sensitivity of the vas deferens to various agonists.the effect of naloxone in vitro, on noradrenaline (na)-induced responses in guinea-pig isolated vas deferens was studied. the responses of na were potentiated by naloxone (3 microm). it potentiated the responses to methoxamine which has least affinity for uptake sites. responses induced by acetylcholine and potassium chloride were also potentiated by naloxone. low calcium (0.8 mm) in the medium antagonized the potentiating action of naloxone. it is suggested that naloxone produces non-specific p ...197936460
the influence of cyclic gmp on contractions of guinea-pig bronchial muscle. 197936475
[effect of benemycin and sodium succinate on metabolism of protons in mitochondria of guinea pig lung and liver tissues].the data of studies on proton metabolism in mitochondria of the guinea pig lungs and liver with application of benemycin and sodium succinate show that benemycin does not affect the metabolism of protons in the lungs and liver mitochondria. sodium succinate activates the release of protons from the lungs and liver mitochondria in state 4 according to chance. when using sodium succinate for reducing the effect of benemycin on theorganism it is found that sodium succinate inhibits the utilization ...197936703
[biochemical changes in guinea pigs after the application of high doses of ascorbic acid].the effect of high doses of ascorbic acid (aa) (200 mg/kg body weight, s. c.) on the values of some biochemical parameters has been studied in guinea pigs (glucose, lactic acid, sh substances and gsh in blood, proteins and urea, and the ldh, mdh, asat, alat, and gamma-gt activities in the serum, the acid phosphatase activity in the liver, the gamma-gt activities in the liver and the kidney, and on the levels of sh substances and gsh in various organs). ascorbic acid was administered to the anima ...197836729
subcellular localization of gamma-glutamyltransferase activity in guinea pig liver. effect of phenobarbital on the enzyme activity levels.the localization of gamma-glutamyltransferase activity in guinea pig liver was studied after subcellular fractionation. the enzyme activity was essentially connected with plasma membranes whereas only low activity was found in the endoplasmic reticulum. a similar activity distribution was demonstrated for 5'-nucleotidase. highest specific activity of gamma-glutamyltransferase was found in plasma membranes enriched in bile canaliculi. in this fraction the specific activity was 35 times greater th ...197937006
histamine receptors on guinea-pig alveolar macrophages: chemical specificity and the effects of h1- and h2-receptor agonists and antagonists. 197937009
effects of catecholamine treatment as well as cold exposure on mitochondrial enzyme activities of the adipose tissue in a guinea-pig (cavia porcellus). 197937034
subcellular distribution and properties of intestinal histamine-metabolizing enzymes from rats, guinea pigs and rhesus monkeys. 197937037
inhibition of guinea-pig brain tyrosine hydroxylase by catechols and biopterin. 197937286
uptake of 5-hydroxytryptamine in blood platelets and its inhibition by drugs: role of plasma membrane and granular storage.the initial uptake of 3h-5-hydroxytryptamine (3h-5-ht) showed linearity for short time intervals in normal and reserpinized blood platelets of guinea-pigs, but was lower in reserpinized platelets. teh km values for the 3h-5-ht uptake were virtually identical in normal and reserpinized platelets, whereas vmax was lower in the latter. imipramine and chlorpromazine caused the same percentage inhibition of 3h-5-ht uptake in normal and reserpinized platelets; the reserpine-like compound ro 4-1284 inh ...197937296
the reversal by pyridostigmine of neuromuscular block produced by soman.the effect of pyridostigmine on neuromuscular block produced by soman was studied in the isolated phrenic nerve-diaphragm preparation. in the rat, soman produced an irreversible reduction in tetanic tension and functional acetylcholinesterase (ache) activity. pretreatment with pyridostigmine before exposure of the diaphragm to soman, followed by removal of the anticholinesterase from the organ bath, produced a return of tetanic tension and an increase of 5% in functional ache activity. similar r ...197937297
comparative metabolism of codeine in man, rat, dog, guinea-pig and rabbit: identification of four new metabolites.the metabolism and excretion of codeine and its metabolites in untreated urine of man, rat, dog, guinea-pig and rabbit have been examined. metabolites were identified by gas chromatography mass spectrometry operated in the chemical ionization mode (methane). concentrations of codeine and metabolites were measured by selected ion monitoring. both codeine and norcodeine were detected in the urine of all species but a new metabolite, hydrocodone, was found only in the urine from man, guinea-pig and ...197937301
31p nuclear magnetic resonance measurement of cardiac ph in perfused guinea-pig hearts. 197937345
histamine receptors mediating cyclic amp accumulation in slices from guinea-pig hippocampus: characterization and localization [proceedings]. 197937727
[antihistaminic activity of tiemonium methylsulfate on isolated guinea pig ileum and trachea]. 197837856
the inhibitory effects of presynaptic alpha-adrenoceptor agonists on contractions of guinea-pig ileum and mouse vas deferens in the morphine-dependent and withdrawn states produced in vitro.1 isolated ilea from guinea-pigs implanted with morphine pellets were stimulated coaxially, either with or without morphine present in the bath fluid, and the longitudinal contractions recorded. 2 in the absence of morphine the inhibitory effects of the presynaptic alpha-adrenoceptor agonists, clonidine and oxymetazoline were much reduced and the dose-response curve was flat. this state of 'withdrawal' was readily reversed by morphine and levorphanol but not its inactive (+)-isomer, dextrophan. ...197937965
chemical synthesis and biological occurrence of carboxylic acid metabolites of delta1(6)-tetrahydrocannabinol.potential metabolites of (-)-3,4-trans-tetrahydrocannabinol (thc) with a carboxylic acid function in the side chain were synthesized as their methyl esters in the delta1(6)-series. the chromatographic and mass-spectrometric properties of the five side-chain homologues were examined and utilized to facilitate the isolation and the identification of the corresponding monocarboxylic acid metabolites of delta1(6)-thc in the mouse, guinea pig, and rabbit formed after ip administration. the metabolite ...197938086
[pharmacological activity of n-basic substituted derivatives of 1,3-dimethylxanthine].the authors carried out studies on cats, guinea pigs, rats and mice, using isolated smooth muscle organs (bronchi, ileum of guinea pig), and examined bronchodilatating, diuretic, antihistaminic action and acute toxiticy of three n-basic substituted derivatives of 1,3-dimethiylxanthine (theophyline) namely: 7-bis-(2-hydroximethyl)-amino-ethyl theophyline under the form of a base (compound g111, tartarate (g112) and nicotinate (g113). the studies were performed in comparison with theophyline. it w ...197938099
thermal responsiveness of 3-hydroxy-3-methylglutaryl-coenzyme-a reductase and acetyl-coenzyme-a carboxylase in neoplastic guinea pig lymphocytes (l2c). 197938110
action of putative neurotransmitters in the guinea pig cochlea.scala tympani of the guinea pig was perfused with artificial perilymph containing putative transmitter substances. single auditory nerve fibre activity was recorded during these perfusions and the responses of these fibres were studied using computer-controlled routine. acetylcholine significantly inhibited tone-induced responses of a large proportion of the fibres tested. glutamate appeared to be a potent depolarising agent when applied in concentrations between 2 and 10 mm. noradrenaline did n ...197938131
alpha- and beta-adrenergic mechanisms in the aorta of newborn rabbits and guinea-pigs. 197938184
specific fluorescent labeling of chicken myofibril z-line proteins catalyzed by guinea pig liver transglutaminase.guinea pig liver transglutaminase has been found to catalyze the covalent incorporation of dansylcadaverine into chicken skeletal muscle myofibril proteins. epifluorescence microscopy reveals that the incorporated dansylcadaverine is specifically localized at or near the myofibril z line. sds-polyacrylamide gel electrophoresis (sds-page) indicates that actin constitutes a major fraction of the labeled material; the z-line proteins alpha-actinin and desmin also show significant labeling, as well ...197938257
correlations between variations of electrical field at the body surface and contractions of the stomach in guinea pig.1. the slow potentiel changes of the base line of the electrocardiogram recorded by cutaneous electrodes, here called egeg (electrogastroenterogram) have been recorded simultaneously with the electromyogram and pressure variations of the stomach antrum. 2. periodic fluctuations of egeg, 400 microv amplitude, were correlated with activities of the antrum emg consisting of 3 to 6 mv spikes superimposed on slow wave of depolarization, and with gastric intraluminal pressure of 5 to 7 cm of water. 3. ...197838335
microsomal metabolism of arylamides by the rat and guinea pig--ii. oxidation of 3-fluorenylacetamide at carbon atom 9. formation of 3-acetamido-9-fluorenone. 197938792
effect of secretin on histamine-induced duodenal ulceration in guinea-pigs. 197939144
effect of antiarrhythmic and analgesic drugs on the effective refractory period of guinea-pig isolated atria and ventricular strips. 197939146
ascorbate destruction of opiate stereospecific binding in guinea pig brain homogenate. 197939241
a simple and efficient method for preparation of immunoelectrophoretically pure guinea pig igm and isolation of monospecific anti-igm antibodies.this report describes experimental conditions developed for the rapid and efficient preparation of guinea pig igm in a purified form and in high yield. a combined immunization procedure had to be designed to obtain sufficiently high igm concentrations in guinea pig serum. the isolation procedure includes precipitation at low ph, followed by sedimentation at 110,000 g and reverse flow gel chromatography on sephades g-200. furthermore,we describe in detail the isolation of relatively large amounts ...197939323
detection of "alpha-type" inhibitors in the presence of low levels of specific influenza native, heated or otherwise treated egg white and in sera of men and guinea pigs, haemagglutination inhibition titres were determined against three inhibitor-sensitive (is) strains and one inhibitor-resistant (ir) variant on influenza a virus. a few human sera with no detectable antibody revealed high inhibition titres even against the ir variant. human sera after treatment with trypsin and periodate revealed mostly a reduction or no change, and exceptionally an increase of their inhibition t ...197939434
long-term culture of guinea pig tongue cells: a suitable interferon system.the interferon system was investigated in a long-term culture of guinea pig tongue (gpt) cells. interferon synthesis was induced with newcastle disease virus (b strain) and f2 phage double-stranded rna. guinea pig interferons were about 2 times more active in cell cultures established from guinea pig embryos than in the gpt cells. human leukocyte and mouse peritoneal cell interferons were active in this cell system also.197939441
aminoglycoside antibiotics and sympathetic ganglionic transmission.the effects of streptomycin, gentamicin, tobramycin and amikacin on transmission in sympathetic ganglia have been studied using the guinea pig isolated hypogastric nerve-vas deferens preparation. these aminoglycoside antibiotics produce dose-related sympathetic blockade at concentrations greater than the common therapeutically effective antibiotic concentrations.197939462
[on the narco-hypnotic of o-chloro 2-phenyl ethyl allophanate (s. c. s. 100) in animal (author's transl)].oral administration of o-chloro 2-phenyl éthyl allophanate (s. c. s. 100) to male and pregnant, or not, female mice, to male and female rats, to male guinea-pigs and rabbits induces, according to tested doses and animal species, a reduction of motoricity, a state of sleepiness giving way to exterior solicitations, a loss of balance, a diminution of muscular tonus or a temporary loss of righting reflex going with narcosis and a reversible suppression of sensibility. the narco-hynoticaction of s. ...197939471
decrease in dose requirement of d-tubocurarine by volatile anesthetics.volatile anesthetics are known to decrease the requirements for neuromuscular blocking agents. to obtain a quantitative measure of the extent of this drug interaction, studies were performed on isolated guinea pig nerve--lumbrical muscle preparations exposed to methoxyflurane, halothane, isoflurane, diethyl ether, fluroxene, and enflurane in concentrations equal to mac. from the relationship between indirect twitch height and d-tubocurarine concentration, the concentration depressing the twitch ...197939476
beta-adrenergic blocking action of halonitrophenethanolamines.a series of phenethanolamines with n-isopropyl and n-tertbutyl substituents and ring-substituted with nitro- and halogen groups has been prepared. using guinea-pig isolated atrial and tracheal preparations, the influence of the nitro-group on the beta 1- and beta 2-antagonist actions of the mono-halogen compounds was determined, and the antagonist and partial agonist effects of the halo-nitro-compounds on beta-adrenoceptors in these tissues measured to help elucidate structure-activity relations ...197939529
effect of strophantin on the isolated guinea-pig ileum.the effect of strophantin on isolated guinea-pig ileum was investigated. it was found that strophantin produces a dose-dependent contraction that is not blocked by atropine, ganglionic blockade, but is potentiated by prostigmine. depolarization of the guinea-pig ileum by a high potassium solution does not change the effect of strophantin. strophantin response is competitively antagonized by verapamil. the effect is significantly reduced, 5 to 10 min, after the preparation is immersed in zero cal ...197939531
[on the mechanism of the positive inotropic effect of triamterene on the myocardium (author's transl)].the influence of triamterene on the contractile force and the cellular electrolyte content and exchange was studied in isolated, electrically stimulated guinea-pig atria. 1. triamterene (40 to 200 mumol/l) produces a positive inotropic effect which is most pronounced when the extracellular calcium concentration is reduced (1.2 to 0.6 mmol/l) or the contractile force is diminished by pentobarbital (400 mumol/l). the inotropic action of triamterene was shown to be independent of the rate of stimul ...197939580
comparison of the beta-adrenoceptor effects of soterenol and its 3-hydroxy, 4-sulphonamido isomer (mj6987-1) in isolated tissues from the guninea-pig.1. soterenol and its 3-hydroxy, 4-methanesulphonamido isomer (mj6987-1) were compared with isoprenaline for beta-adrenoceptor mediated effects in guinea-pig atrial, tracheal, uterine and ileal preparations. in addition, mj6987-1 was tested for its effects in the atria of cats, rabbits and rats. 2. soterenol had a lower intrinsic activity and was approximately two to six times less active than isoprenaline in all preparations. 3. mj6987-1 was a full agonist, being some 30--200 times less active t ...197939696
fibrin membrane endowed with biological function. iv. formation of cross-links between fibrinogen (or fibrin) and ribonuclease by transglutaminase.transglutaminase from guinea pig liver catalyzed the formation of cross-links between fibrinogen (or fibrin) and ribonuclease. using transglutaminase, immoblized ribonuclease was prepared by two separate methods: (1) fibrinogen-ribonuclease conjugates formed by transglutaminase were treated with thrombin to make fibrin membrane bound covalently to the enzyme; (2) fibrin polymer formed from fibrinogen with thrombin was covalently bound to ribonuclease by transglutaminase to make fibrin-ribonuclea ...197939750
[preclinical study of the safety of a new tranquilizer, phenazepam].chronic toxicity of the benzodiazepine series tranquilizer phenazepam (5 and 50 mg/kg) administered per os for 1.3 and 6 months was studied in experiments on white rats, guinea pigs and dogs. it has been shown during varying time of observation that the drug neither decreases weight gain in the animals nor adversly acts on the blood or produces local irritating action. histopathological changes in some internal organs proved irreversible. administered at varying periods of pregnancy, phenazepam ...197939773
placental clearance of lactate and bicarbonate in determine the placental clearance of lactate and bicarbonate in sheep, the fetal side of an isolated cotyledon and the umbilical circulation of the total placenta were artificially perfused. the release and uptake of lactate and bicarbonate by the perfusion fluid and the fetomaternal concentration differences of these substances were measured. from these data, the clearance of lactate and bicarbonate was determined to be 0.9 (se = 0.2) ml/h/g of placental tissue. the production of lactate by ...197939880
Displaying items 401 - 500 of 99137