PMID(sorted ascending)
discrimination by female mice between the odours of parasitized and non-parasitized males.the detection and avoidance of parasitized males has been proposed to be a component of female mate choice. we investigated whether or not female laboratory mice, mus musculus domesticus, could discriminate between parasitized and non-parasitized males on the basis of odour. female mice were given a choice between the urine and other odorous secretions of either a male mouse sub-clinically infected for five days with the naturally occurring, enteric, single host, protozoan parasite, eimeria verm ...19957644547
population subdivision and gene flow in danish house mice.genetic subdivision in local populations of the european house mice, mus musculus domesticus and m. m. musculus, was analysed to study patterns of gene flow. the data consisted of frequencies of microsatellite alleles in 16 samples (250 individuals) from a total of 11 sites in jutland, which included successive samples from three sites. sequences of the control region of mitochondrial dna in three successive samples from one site were also analysed. microsatellite genotype frequencies within sam ...19957663750
circular transcripts of the testis-determining gene sry in adult mouse testis.sry is expressed at higher levels in the adult testis, where no function has been determined, than in the genital ridge, its critical site of action. cdna and 5' race clones isolated from testis or from sry-transfected cell lines have an unusual structure, with 3' sequences located in a 5' position. rnaase protection assays and reverse transcription polymerase chain reactions confirmed that these unusual rna molecules represent the most abundant transcript in testis. furthermore, oligonucleotide ...19937684656
distribution of acetylcholinesterase in the hippocampal region of the mouse. iii. the area dentata.the distribution of acetylcholinesterase (ache) was examined in the area dentata of the adult mouse (mus musculus domesticus). a distinctly stratified distribution of the enzyme was observed and was compared in detail with cytoarchitectural fields and layers. in the stratum moleculare, bands of relatively high ache activity were seen immediately beneath the pia, at the borders between the outer, middle, and deep portions of the stratum moleculare, and superficial to the granule cell layer. ache ...19937685362
sibling effects on the behavior of infant mouse litters (mus domesticus).we investigated whether the number of same- or other-sex littermates had a graded effect on the occurrence of early solitary play and social play by mouse (mus domesticus) family units. sixty litters, reduced at birth to 5 different sex ratios (6 males, 5 males and 1 female, 3 males and 3 females, 1 male and 5 females, and 6 females) were scored in 15-min sessions on postnatal days 18 and 21. an increasing trend with age was found for run, pounce, popcorn, self-groom, and explore episodes. type ...19957705064
length variation of cag repeats in sry across populations of mus domesticus. 19957749230
the effect of sub-lethal doses of bromadiolone on the breeding performance of house mice (mus domesticus).both laboratory and field strains of mus were exposed to wheat containing 0.0001% bromadiolone under laboratory and outdoor conditions, respectively. while both strains readily consumed the poisoned wheat, ad libitum sub-lethal doses of this anticoagulant equating to between 20% and 70% of the acute ld50 per feed had little apparent effect on the breeding performance of these mice. the implications of these findings are discussed with respect to the use of anticoagulants as pesticides.19957749606
aging lengthens circadian period for wheel-running activity in c57bl mice.previous reports of age effects on circadian period in rodents show a slight shortening of period with age, with the exception of house mice (mus domesticus) where a number of studies report mixed results. the present study consists of three comparisons of circadian period for wheel-running activity in young vs. older c57bl inbred mice following entrainment to 16:8 ld, 12:12 ld and 8:16 ld photoperiods. the free-running circadian period (tau(dd)) for wheel-running activity was significantly long ...19957753897
the hsr on chromosome 1 of the house mouse, mus domesticus: distribution and frequency in switzerland.a total of 357 house mice (mus domesticus) from 83 localities uniformly distributed throughout switzerland were screened for the presence of a homogenously staining region (hsr) on chromosome 1. altogether 47 mice from 11 localities were hsr/+ or hsr/hsr. one sample of 11 individuals all had an hsr/hsr karyotype. almost all mice with the variant were collected from the rhone valley (hsr frequency: 61%) and val bregaglia (hsr frequency: 81%). for samples from most of the area of switzerland, the ...19947813901
distribution of the molossinus allele of sry, the testis-determining gene, in wild mice.when the y chromosome of the laboratory inbred mouse strain c57bl/6 (b6) is replaced by the y of certain strains of mus musculus domesticus, testis determination fails and all xy fetuses develop either as hermaphrodites or xy females (xy sex reversal). this suggests the presence of at least two alleles of sry, the male-determining gene on the y:m. m. domesticus and b6. the b6 y chromosome is derived from the japanese house mouse, m. m. molossinus and therefore carries a molossinus sry allele. as ...19947815926
the potential of murine cytomegalovirus as a viral vector for immunocontraception.wild populations of house mice (mus domesticus) regularly undergo population eruptions in the cereal growing regions of s.e. australia, causing significant damage to crops. rodenticides are costly and are of limited usefulness, and the need for a biological control agent is widely recognized. the options for the use of an infectious agent to control mouse populations are considered. the three main types are: infectious agents which establish lethal infection; those which directly interfere with ...19947831489
assignment of tp53 and tk1 to chromosome 13 in peromyscus by fluorescence in situ hybridization.mus domesticus dna probes for the tumor suppressor protein-53 (tp53) and thymidine kinase-1 (tk1) genetic loci were used to identify clones representing these loci in a peromyscus leucopus (white-footed mouse) cosmid library. the cosmid-derived homologous probes were biotinylated and hybridized to p. maniculatus (deer mouse) chromosomes. probes for both genes hybridized to the chromosome 13 pair identified by prior g-banding. deer mouse chromosome 13 shares a region of homology with mouse chromo ...19957835097
plasma alpha-1-antiproteinase from the mongolian gerbil, meriones unguiculatus: isolation, partial characterization, sequencing of cdna, and implications for molecular evolution.alpha-1-antiproteinase (also called alpha-1-proteinase inhibitor or alpha-1-antitrypsin) with a molecular mass of 56 kda was purified from plasma of the mongolian gerbil, meriones unguiculatus, to apparent homogeneity. it inhibited trypsin, chymotrypsin, elastase, and plasmin, but not kallikrein or thrombin. eight cdna clones coding for this protein were isolated from a liver cdna library and sequenced. they contained the same coding regions consisting of a 24-residue signal peptide and a 382-re ...19947852275
factors that may regulate assembly of the mammalian sperm tail deduced from a mouse t complex mutation.a unique pattern of aberrant sperm development occurs in laboratory mice (mus domesticus) that have been made homozygous for an allele of an autosomal gene, hst-6s, derived from another mouse species, mus spretus. during the abnormal spermiogenic process of these sterile animals, the sperm tail fails to assemble normally, although a bud develops at the site of the centriole in early spermatids. the bud enlarges during sperm development and eventually contains elements of a mature sperm tail. tub ...19937904484
developmental arrest of fertilized eggs from the b6.ydom sex-reversed female mouse.when the y chromosome of a mus musculus domesticus mouse strain is placed onto the c57bl/6j (b6) inbred background, the xy progeny develop ovaries or ovotestes but never normal testes during fetal life. while some of the hermaphroditic males become fertile, none of the xy females produces litters. here, we examined the fertility and development of oocytes derived from the xy female mouse. with or without preceding injection of gonadotropins, female mice were mated with normal b6 males, and their ...19947955569
normal onset, but prolonged expression, of sry gene in the b6.ydom sex-reversed mouse gonad.when the y chromosome of the mus musculus domesticus mouse (tirano, italy) is placed onto the c57bl/6j (b6) background, the xy progeny (b6.ydom) develop only ovaries or ovotestes in fetal life. in the present study, we examined transcription of genes known to be involved in gonadal sex differentiation in the b6.ydom gonad. rna was isolated from each gonad at 10 to 18 days of gestation and subjected to rt-pcr analysis. the results indicate that the onset of sry, a putative testis-determining gene ...19947958412
genetic analysis of liver tumorigenesis in sv40 t antigen transgenic mice implies a role for imprinted genes.liver tumors from interspecific hybrid, transgenic mice containing the sv40 early region linked to a mouse major urinary protein enhancer/promoter were analyzed for loss of heterozygosity to identify chromosomal regions which potentially contain genetic loci involved in multistep tumorigenesis. a broad pattern of complete and partial loss of heterozygosity or allelic imbalance was observed with frequent loss of heterozygosity/partial loss of heterozygosity of loci on chromosomes 1, 5, 7, 8, and ...19947987847
fluctuating asymmetry in the mus musculus hybrid zone: a heterotic effect in disrupted co-adapted genomes.developmental stability reflects the organism's ability to buffer minor developmental accidents and is often estimated by measuring the fluctuating asymmetry. either implicitly or explicitly, numerous authors have assumed that developmental stability is correlated with overall fitness. if this is the case, changes in morphological asymmetry across a hybrid zone could be used as a measure of the selection on hybrid genomes. developmental stability in hybrid populations is theoretically related to ...19947997458
the original pink-eyed dilution mutation (p) arose in asiatic mice: implications for the h4 minor histocompatibility antigen, myod1 regulation and the origin of inbred strains.allelic variation of the mouse pink-eyed dilution (p) gene in common laboratory strains and wild mice was examined by southern blot and by polymerase chain reaction. in these assays the original p mutation allele found in strains sjl/j, 129/j, b10.129(21m), p/j and fs/ei most closely matches an asian mus musculus allele, confirming anecdotal accounts of the asian origin of this mutation. in contrast, the wild-type allele found in other common laboratory strains was apparently derived from mus do ...19948001787
mitochondrial dna variation and the evolution of robertsonian chromosomal races of house mice, mus domesticus.the house mouse, mus domesticus, includes many distinct robertsonian (rb) chromosomal races with diploid numbers from 2n = 22 to 2n = 38. although these races are highly differentiated karyotypically, they are otherwise indistinguishable from standard karyotype (i.e., 2n = 40) mice, and consequently their evolutionary histories are not well understood. we have examined mitochondrial dna (mtdna) sequence variation from the control region and the nd3 gene region among 56 m. domesticus from western ...19948005418
polymorphism of a cag trinucleotide repeat within sry correlates with b6.ydom sex reversal.breeding the y chromosome from certain mus musculus domesticus strains onto the inbred laboratory mouse strain, c57bl/6j (b6), results in hermaphroditic progeny. this strain-dependent sex reversal suggests that there may be significant allelic variation in the murine sex determining gene, sry. we have analysed the sry genes from several domesticus-type y chromosomes and show that they encode smaller proteins than the molossinus-type alleles sryb6 and sry129. we have also identified a polymorphic ...19948012385
polymorphism and divergence at the 5' flanking region of the sex-determining locus, sry, in mice.we have investigated patterns of evolution in the nonrecombining portion of the y chromosome in mice by comparing levels of polymorphism within mus domesticus with levels of divergence between m. domesticus and m. spretus. a 1,277-bp fragment of noncoding sequence flanking the sex determining locus (sry) was pcr amplified, and 1,063 bases were sequenced and compared among 20 m. domesticus and 1 m. spretus. two polymorphic base substitutions and two polymorphic insertion/deletion sites were ident ...19948015446
nonneutral evolution at the mitochondrial nadh dehydrogenase subunit 3 gene in mice.the neutral theory of molecular evolution asserts that while many mutations are deleterious and rapidly eliminated from populations, those that we observe as polymorphisms within populations are functionally equivalent to each other and thus neutral with respect to fitness. mitochondrial dna (mtdna) is widely used as a genetic marker in evolutionary studies and is generally assumed to evolve according to a strictly neutral model of molecular evolution. one prediction of the neutral theory is tha ...19948022788
shared sequence variants of mus spretus line-1 elements tracing dispersal to within the last 1 million years.line-1 repetitive sequences contain a record of an evolving population of transposons within the mammalian genome. of the 100,000 copies of line-1 sequences per genome there are many shared sequence variants representing changes occurring within the propagating line-1 elements themselves, rather than changes that occur during retrotransposition or after an element inserts in the genome. these shared sequence variants define families of line-1 elements which have spread within specific periods of ...19948070666
sequences and evolutionary analysis of mouse 5s rdnas.we selectively amplified the spacer regions of genes for mouse 5s ribosomal rna (rrna), which are tandemly repeated, by the pcr method, using primers specific to the two ends of the coding region for 5s rrna. fragments of approximately 1.6 kb were amplified from dna from the balb/ccrslc mouse (mus musculus domesticus), the sm/j mouse (m. m. domesticus), the moa mouse (m. m. musculus) and the seg mouse (m. spretus). these fragments were cloned into an appropriate plasmid vector, and two clones re ...19948078409
polymorphism, localization and geographical transfer of mitochondrial dna in mus musculus domesticus (irish house mice).the analysis of mtdna restriction fragments from irish house mice revealed much polymorphism, both within and between populations. many phenotypes showed geographical localization and there was a strong correlation between geographical distance and genetic divergence. populations, which are discontinuous and limited to buildings or their vicinity, are apparently the result of short-range migration. transport by man, with whom the species is closely associated, appears to have negligible impact. ...19938094386
restriction fragment length polymorphism and evolution of the mouse immunoglobulin constant region gamma loci.genomic dna from twelve laboratory mouse strains, in addition to 21 wild-derived strains belonging to different taxa (mus musculus domesticus, mus musculus musculus, mus spretus, mus macedonicus, and mus spicilegus) and four mouse strains that are evolutionarily more distant, were analyzed by southern blot for polymorphism of the ig heavy chain constant region gamma isotype (igh-c gamma) and for the distribution of the duplicated igh-1 (c gamma 2 alpha) haplotype. distinct allelic forms of each ...19938099342
molecular and biological characterization of new strains of murine cytomegalovirus isolated from wild mice.studies of the prevalence of antibody to murine cytomegalovirus (mcmv) in free-living wild mice (mus domesticus) trapped in diverse regions of australia and on a sub-antarctic island indicated that 90% of 468 mice had serum antibody to mcmv. twenty-six field isolates of mcmv were plaque-purified from salivary gland extracts of representative seropositive mice. these isolates varied considerably in their ability to replicate in the salivary glands of weanling balb/c mice with 9 of 15 failing to r ...19938102523
sex differences in plasma corticosterone in mouse fetuses are mediated by differential placental transport from the mother and eliminated by maternal adrenalectomy or stress.the effect of changes in maternal corticosterone concentrations, induced by maternal stress, maternal adrenalectomy or both, on concentration of corticosterone in serum and in adrenals of mouse (mus domesticus) fetuses was examined. higher baseline serum corticosterone concentrations were found in female fetuses than in male fetuses; however, there was no sex difference in the content of corticosterone in adrenals collected from these fetuses. sex differences were observed in the fetal response ...19938107008
association of xmv-10 and the non-agouti (a) mutation explained by close linkage instead of a previous survey of endogenous proviruses among inbred mouse strains, the xmv-10 provirus was found only in strains that carried the non-agouti (a) mutation (frankel et al. j. virol. 63: 1763-1774, 1989). to determine whether insertion of xmv-10 caused the a mutation, we cloned a portion of xmv-10 and its insertion site. using a fragment of flanking cellular dna as a southern hybridization probe, we found that the xmv-10 provirus was still present in revertant alleles of a to a(t) or aw. a r ...19948111126
genetic control of retroviral disease in aging wild mice.different populations of wild mice (mus musculus domesticus) in los angeles and ventura counties were observed over their lifespan in captivity for expression of infectious murine leukemia virus (mulv) and murine mammary tumor virus (mmtv) and for the occurrence of cancer and other diseases. in most populations of feral mice these indigenous retroviruses were infrequently expressed and cancer seldom occurred until later in life (> 2 years old). mmtv was found in the milk of about 50% of wild mic ...19938125269
genetic mediation of infanticide and parental behavior in male and female domestic and wild stock house mice.infanticide is a reproductive strategy found in many mammals, especially rodents. the proportion of male and female house mice (mus domesticus) that are either infanticidal or noninfanticidal is strain specific and varies widely from stock to stock. male house mice also show strain-specific variation in the behavioral mechanisms that inhibit infanticidal individuals from killing their own offspring. the adult offspring generated from reciprocally crossed cf-1 and wild stock house mice were teste ...19938129694
biochemical basis of warfarin and bromadiolone resistance in the house mouse, mus musculus domesticus.danish mice (mus musculus domesticus) genetically resistant to the anticoagulant action of two 4-hydroxycoumarins, warfarin and bromadiolone, were examined to determine their mechanism of resistance. the hepatic vitamin k epoxide reductase in the bromadiolone-resistant mice and in one phenotype of warfarin-resistant mice was highly insensitive to in vitro inhibition by warfarin and bromadiolone. the kinetic constants for the epoxide reductase from bromadiolone-resistant mice were also altered. t ...19948129744
phenotype and tcr gamma delta variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice (mus musculus domesticus): abundance of v gamma 1 transcripts and extensive delta gene order to study murine intestinal intraepithelial lymphocytes (iel) independent of factors imparted by conditions of laboratory housing and breeding, and to provide a basis for comparison of iel studies between inbred and outbred mouse populations, iel from the domestic house mouse, mus musculus domesticus, were analyzed by flow cytometric analyses using mabs to murine lymphocyte markers, and by polymerase chain reaction to study the tcr gamma and delta v gene repertoires. the majority of iel ...19948155599
polymorphism of the mouse transcription factor-encoding gene e2a.southern blotting analysis using a cdna probe consisting of the central portion of the e12 coding region has revealed two distinct forms of e2a, one which is common to all inbred and wild mouse strains derived from mus musculus musculus and mus musculus domesticus, whereas the other is less common and has only been found in the wild mouse population of mus musculus domesticus.19948163208
inhibition of sexual maturation in juvenile female and male mice by a chemosignal of female origin.maturation of juvenile house mice (mus domesticus) exposed to synthetic 2,5-dimethylpyrazine was compared to the maturation of juvenile male and female mice exposed to urine from adult males and grouped females. exposure of juvenile male mice to the synthetic compound significantly inhibited the growth of their testes and sex-accessory glands in a fashion similar to the urine of adult males and grouped females. the uterine weight and the presence of ova in the oviducts in females was strongly af ...19948190771
the testis-determining autosomal trait, tda-1, of c57bl/6j is determined by more than a single autosomal gene when compared with dba/2j mice.the putative tda-1 or testis-determining autosomal trait of the c57bl/6j mouse strain came to attention when the y chromosome from the poschiavinus variety of mus musculus domesticus was introduced into c57bl/6j by backcross matings. the f1 generation expressed normal testis development in xy individuals with the poschiavinus y chromosome. in the backcross and subsequent crosses to c57bl/6j females, xy individuals expressed ovaries bilaterally or various combinations of an ovotestis with a contr ...19948200518
cloning and sequence analysis of the gene and cdna encoding mouse spermidine/spermine n1-acetyltransferase--a gene uniquely regulated by polyamines and their analogs.the polyamine catabolizing enzyme, spermidine/spermine n1-acetyltransferase (ssat), has been implicated as a critical determinant of polyamine pool maintenance. ssat has recently been shown to be positively regulated in human cell lines by polyamines and their analogs at the level of mrna accumulation. mouse la-4 lung adenoma cells treated with either spermine or the spermine analog, n1,n12-bis(ethyl)spermine, produced a 2.3 and 6.5-fold increase, respectively, in ssat mrna. prior evidence for t ...19938241266
characterizations of candidate genes for idd susceptibility from the diabetes-prone nod mouse strain.the nucleotide sequences of the nod and c57bl/6j alleles of glut-2, sod-2, and il-2 were determined by rt-pcr sequencing. each of these loci is located in intervals that strongly correlated with susceptibility to diabetes in an (nod/uf x c57bl/6j)f1 x nod/uf backcross. no significant variations in the alleles of glut-2 at 16 cm on chromosome (chr) 3 or sod-2 at 8 cm on chr 17 were detected. however, the il-2 allele in nod at 20 cm on chr 3 was found to differ from that in c57bl/6j by a complex m ...19938268651
further studies of a staggered hybrid zone in mus musculus domesticus (the house mouse).in the extreme north-east of scotland (near the village of john o'groats) there is a small karyotypic race of house mouse (2n = 32), characterized by four metacentric chromosomes 4.10, 9.12, 6.13 and 11.14. we present new data on the hybrid zone between this form and the standard race (2n = 40) and show an association between race and habitat. in a transect south of john o'groats we demonstrate that the clines for arm combinations 4.10 and 9.12 are staggered relative to the clines for 6.13 and 1 ...19938276635
population dynamics of aberrant chromosome 1 in mice.natural populations of two semispecies of house mouse, mus musculus domesticus and m.m. musculus, were found to be polymorphic for an aberrant chromosome 1 bearing a large inserted block of homogeneously staining heterochromatin. strong meiotic drive for the aberrant chromosome from m.m. musculus was previously observed in heterozygous female mice. there are at least three meiotic drive levels determined by different allelic variants of distorter. homozygotes had low viability and females showed ...19938314713
experimental evidence of genetic determinism in high susceptibility to intestinal pinworm infection in mice: a hybrid zone the hybrid zone of the two mouse subspecies mus musculus musculus and mus musculus domesticus, mice with hybrid genotypes harbour, on the average, more helminth parasites (cestodes and nematodes) than mice of the two parental taxa. in order to determine the roles played by genetic parameters in this phenomenon, mice with recombined and parental genotypes were experimentally infected with the intestinal pinworm aspiculuris tetraptera, a natural parasite of the house mouse. the results showed t ...19938316436
an ephemeral sex pheromone of female house mice (mus domesticus): pheromone fade-out time.male mice will emit ultrasounds to at least two chemosignals present in female mouse urine: a) an ephemeral unconditioned stimulus (ucs) present in freshly voided urine and b) a conditioned stimulus (cs) that remains after the ucs has disappeared. in this study, four phases examined the time frame over which the ucs disappeared. urine that had been aged for either 12, 15, 18, or 24 h was compared for vocalization elicitation with fresh urine (which contains the ucs) and metabolic cage-collected ...19938327598
olfactory discrimination of urinary odortypes from congenic strains (dba/1bg and dba1.c57bl10-ybg) of mice differing in their y chromosomes.differential effects of one or more genes in the nonpseudoautosomal region of the dba1 and c57bl10 y chromosomes on offensive attack may be mediated, at least in part, by differential effects of this y chromosomal gene(s) on the sending and receiving of discriminable urinary odortypes. this hypothesis is based, in part, on the report that urine from a pair of y chromosomal congenic strains (c57bl6.akr-y and c57bl6) of mice can be discriminated in a y maze test. the akr and c57bl6 y chromosomes c ...19938352719
further examination of the kinetics of gonadal development in xy female mice.y-chromosomes derived from house mouse populations of mus domesticus are able to lead to the formation of xy females (i.e. hermaphrodite individuals with both ovarian and testicular tissues) when inserted, by appropriate crosses, into the c57bl/6j genome, which usually carries the mus musculus y chromosome. an abnormal interaction between the testis-determining gene on the y (of domesticus origin) and an autosomal recessive allele (of musculus origin) needed for testicular formation is the likel ...19938374503
familiarity with conspecific odor and isolation-induced aggressive behavior in male mice (mus domesticus).male swiss cd-1 mice (mus domesticus, n = 60) were isolated for 24 days. in the isolation group mice were exposed to their own odor; in the familiarity group mice were familiarized with pairmates' odor by daily exchange of bedding; and in the unfamiliarity group subjects (exposed) were familiarized with pairmates' odor, whereas the pairmates (nonexposed) were familiarized with odors of other isolated mice. aggressive behavior was scored during 20-min sessions. familiarity with the pairmate's odo ...19938375149
high-resolution comparative mapping of mouse chromosome 17.we have constructed a high-resolution genetic linkage map spanning the proximal 32 cm of mouse chromosome 17 including the t complex. simple sequence repeats at d17tu1 and d17mit6 were employed to identify 121 recombinants among 374 offspring of a (c57bl/6 x cast/ei) x c57bl/6 backcross. in contrast to previously reported interspecific mus domesticus x mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. this and the relatively high frequency of dna polymorphisms b ...19938406442
new t-cell receptor gamma haplotypes in wild mice and evidence for limited tcrg-v gene polymorphism.tcrg gene polymorphism was investigated by southern blot analysis on a panel of laboratory and wild mouse strains using a set of probes which identify all known tcrg-v and -c genes. only three haplotypes are found in laboratory mice: ga, gb, and gc which are represented by balb/c, akr, and dba/2 prototypes respectively. ga and gc haplotypes are the most frequent among laboratory mice whereas gb is poorly represented. seven new haplotypes are described among 23 wild mice corresponding to four mus ...19938420824
region-specific rates of molecular evolution: a fourfold reduction in the rate of accumulation of "silent" mutations in transcribed versus nontranscribed regions of homologous dna fragments derived from two closely related mouse species.we have sequenced homologous dna fragments of 2.7 and 2.8 kbp derived from the closely related mouse species mus musculus domesticus (m. domesticus) and mus musculus musculus (m. musculus), respectively. these two species diverged approximately 1 million years ago. each dna fragment contains 1.35 kbp of the 3' end of the constitutively expressed 2.2-kbp aprt (adenine phosphoribosyltransferase) gene and a similarly sized nontranscribed region downstream of the aprt gene. the aprt gene region cont ...19938433377
immunoglobulin constant kappa gene alleles in twelve strains of mice.genomic dna for the immunoglobulin (ig) constant kappa igk-c gene region was amplified by the polymerase chain reaction (pcr) method and sequenced from twelve commonly used inbred mouse strains. pcr products were used directly as templates in dideoxy-dna-sequencing, a method which avoids the sequencing errors caused by taq polymerase, since no cloning step is required. in restriction fragment length polymorphism (rflp) studies the sjl mouse strain has been shown to belong to a igk-c allogroup di ...19938436414
mus spretus-specific line-1 dna probes applied to the cloning of the murine pearl locus.line-1 is the major family of long, interspersed, repetitive dna sequences found in mammalian genomes. the mouse species mus spretus contains large line-1 subfamilies that are distinguishable from the line-1 elements of laboratory mus domesticus strains by their content of particular nucleotide differences. oligonucleotides containing these differences act as m. spretus-specific line-1 hybridization probes. we have used these probes as a novel genetic tool in conjunction with an interspecific hy ...19938449493
prevalence of viral antibodies and helminths in field populations of house mice (mus domesticus) in southeastern australia.a 13-month study of wild mice (mus domesticus) in wheatlands in southeastern australia contrasted changes in the seroprevalence of antibody to 13 viruses and the occurrence of helminths with changes in their population dynamics. mice were seropositive for mouse hepatitis virus (mhv), rotavirus, minute virus of mice (mvm), mouse adenovirus (madv), reovirus (reo 3), and murine cytomegalovirus (mcmv). the seroprevalences of all but rotavirus varied significantly with time and increased with host de ...19938472782
a b2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene.a variety of cdna and genomic clones for the gamma-subunit of mouse muscle phosphorylase kinase (phk-gamma m) have been isolated and characterized. the murine gene for phk-gamma m (phkg) exhibits multiple transcription start sites that are identical in skeletal muscle, cardiac muscle, and brain. the gene is composed of 10 exons and includes a 4.9-kb intron located in the 5' untranslated region. two mrna species of 1.75 and 2.55 kb are produced from phkg in icr and c57bl/10 mice; these transcript ...19938486349
a serologic survey for viruses and mycoplasma pulmonis among wild house mice (mus domesticus) in southeastern australia.plasma samples from 267 wild house mice (mus domesticus) trapped at 14 sites in southeastern australia were screened for antibody to 14 viruses normally associated with laboratory-reared rodents and to mycoplasma pulmonis. serologic prevalence was high for murine cytomegalovirus (99%, n = 94), murine coronavirus (95%), and murine rotavirus (74%). samples from mice collected at all sites contained antibody to these viruses. the serologic prevalence was lower for mouse adenovirus, strain k87 (37%) ...19938487371
endocrine differentiation of the xy sex-reversed mouse ovary during postnatal development.when the mouse y chromosome of mus musculus domesticus is placed onto the c57bl/6j genetic background, half of the xy progeny develop bilateral ovaries and the female phenotype, but lack regular estrous cyclicity and lose embryos after fertilization. in the present study, we compared the endocrinological activity of xy ovaries with xx ovaries during postnatal development by measuring steroids in the incubation medium by radioimmunoassay. at 1 day postpartum (d.p.p.), production of progesterone a ...19938499334
targeted cloning of a subfamily of line-1 elements by subfamily-specific line-1-pcr.subfamily-specific line-1 pcr (ssl1-pcr) is the targeted amplification and cloning of defined subfamilies of line-1 elements and their flanking sequences. the targeting is accomplished by incorporating a subfamily-specific sequence difference at the 3' end of a line-1 pcr primer and pairing it with a primer to an anchor ligated within the flanking region. ssl1-pcr was demonstrated by targeting amplification of a mus spretus-specific line-1 subfamily. the amplified fragments were cloned to make a ...19938499652
evaluation of a nematode (capillaria hepatica bancroft, 1893) as a control agent for populations of house mice (mus musculus domesticus schwartz and schwartz, 1943).sudden, large-scale infestations of house mice (mus musculus domesticus) occur irregularly in the cereal-growing regions of australia, resulting in substantial economic losses. mathematical modelling has been used to evaluate the use of the liver nematode capillaria hepatica as a potential agent for the biological control of mouse populations. the models suggest that c. hepatica is unlikely to be successful as a single-release control agent: instead, the parasite would need to be released when i ...19938518449
loss of telomeric sites in the chromosomes of mus musculus domesticus (rodentia: muridae) during robertsonian rearrangements.mouse chromosomes possessing multiple robertsonian rearrangements (rb chromosomes) have been examined using fluorescence in situ hybridization with the telomeric consensus sequence (ttaggg)n. no hybridization signals were detected at the primary constriction of rb chromosomes. this observation leads us to conclude that the formation of rb chromosomes in the mouse is invariably associated with the loss of telomeric regions. more significantly, a further alteration in regions flanking the primary ...19958528584
identification and characterization of a novel conserved dna repeat.homozygous in(10)17rk mice contain a paracentric inversion within chromosome (chr) 10 and exhibit a pygmy phenotype, suggesting that the distal inversion breakpoint is within the pygmy (pg) locus. in order to obtain the pygmy gene by positional cloning procedures, in(10)17rk dna was subjected to rflp analysis with single-copy probes derived from the wild-type pygmy locus. this analysis identified a dna polymorphism in the dba/2j mouse strain on which the in(10)17rk mutation was originally induce ...19958535068
an ige-binding tame esterase in the urine of the mouse, mus musculus domesticus.among the highly heterogeneous components of the mouse urinary protein complex (mup) a hydrolase was detected which was capable of spliting the proteinase ester substrate tame as well as a synthetic chromogenic tripeptide specific for tissue and urinary kallikreins. the binding of mouse-specific ige antibodies from the serum of a highly mouse-allergic patient occurred preferentially to this kallikrein-like enzyme. this finding underscores the possible association of significant biological activi ...19958537097
nest defense and survival of offspring in highly aggressive wild canadian female house mice.nest defense behavior was examined in wild female house mice (mus domesticus) that were derived from a stock initially trapped in alberta, canada. the first objective was to determine whether behavior toward pups prior to mating was related to the intensity of postpartum aggression in a variety of social situations. therefore, prior to the experiments we screened virgin females for their behavior toward a newborn pup [60% of the females exhibited infanticide and 40% were noninfanticidal: 7% were ...19958559775
sex reversal in c57bl/6j-ypos mice corrected by a sry transgene.c57bl/6j mice carrying a mus domesticus poschiavinus y chromosome (ypos) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. we tested the hypothesis that the y-linked component of this inherited sex reversal is caused by the m. d. poschiavinus y-linked testis determining gene (symbolized tdy or sry) by examining gonadal development in c57bl/6j xypos mice carrying a m. musculus allele of sry as a transgene. we found that in the presence of the transgen ...19958570690
the genetic basis of murine and human sex determination: a review.determination of mammalian sex depends on the presence or absence of a functional testis. testes are determined by the activity of the testis determining factor encoded by the sex determining gene, y (sry) located on the y chromosome. considerable evidence suggests that the sry gene is the only gene on the y chromosome that is both necessary and sufficient to initiate testis determination. other steps in the mammalian sex determining pathway are unknown, although recent advances have shown that ...19958575930
maximal sprint speeds and muscle fiber composition of wild and laboratory house mice.we compared males from four groups of house mice (mus domesticus), all bred and raised under common conditions in the laboratory: randombred hsd:icr; a wild population from wisconsin; hybrids from lab dams; hybrids from wild dams. wild mice were much faster sprinters (maximal forced sprint speeds over 1.0 m ranged from 2.38 to 3.34 m/s) than were lab mice (range = 0.89-1.68 m/s). hybrids exhibited intermediate speeds (range = 1.54-2.70 m/s) and body masses, indicating largely additive inheritanc ...19958577882
h-2 polymorphisms are more uniformly distributed than allozyme polymorphisms in natural populations of house mice.patterns of h-2 and allozyme polymorphism in natural populations of house mice from europe, north africa and south america were analyzed. the purpose of the analysis was to determine whether h-2 and allozyme polymorphisms were similarly distributed both geographically and temporally in wild mice. two subspecies of house mice, mus musculus domesticus and m. m. musculus were sampled and the polymorphisms of two h-2 class i genes, h-2k and h-2d, and 34 allozyme-encoding genes were surveyed. the thr ...19888608923
functional comparison of the mus musculus molossinus and mus musculus domesticus sry genes.the sry gene functions as a genetic switch initiating testicular development of the indifferent mammalian gonad. the mus musculus molossinus sry open reading frame (orf) encodes a 395-amino acid transcription factor (msry) that specifically binds and bends dna through its n-terminal hmg domain and activates transcription through its long c-terminal (residues 144-366) glutamine/histidine-rich activation domain. the m. m. domesticus sry orf encodes a highly homologous, truncated protein (dsry) of ...19958614401
absence of correlation between sry polymorphisms and xy sex reversal caused by the m. m. domesticus y chromosome.mus musculus domesticus y chromosomes (ydom chrs) vary in their ability to induce testes in the strain c57bl/6j. in severe cases, xy females develop (xydom sex reversal). to identify the molecular basis for the sex reversal, a 2.7-kb region of sry, the testis-determining gene, was sequenced from ydom chrs linked to normal testis determination, transient sex reversal, and severe sex reversal. four mutations were identified. however, no correlation exists between these mutations and severity of xy ...19968617507
directional dominance and a developmental model for the expression of the tda testis-determining autosomal trait of the mouse.the posch-2 y chromosome from the poschiavinus variety of mus musculus domesticus causes incomplete testis development in the recessive autosomal background of the c57bl/6j laboratory mouse strain. testis development is normal with the posch-2 y in its native strain background as well as in some strains of the laboratory mouse such as dba/2j. the phenotype or expression of xy gonadal hermaphroditism in a c57bl/6j strain, which was constructed to be consomic for the posch-2 y, is a threshold trai ...19968675000
odor preferences of female house mice (mus domesticus) in seminatural enclosures.preferences for male odors by female house mice (mus domesticus) were examined with respect to age, dominance status, and spatial relationships. mice were free living in 6 field enclosures. estrous or nonestrous females were placed in an aquarium with soiled bedding from live traps as the odor source. females were tested for preferences between (a) adult and juvenile male odors, (b) dominant and subordinate male odors, and (c) "near" and "far" male odors. in dominance odor tests, estrous females ...19968681527
corticosterone and interchromosomal race discrimination in the house mouse.we report results of a study on emotional arousal, as reflected by corticosterone variation, following dyadic encounters within and between chromosomal races of the house mouse (mus musculus domesticus). three contiguous chromosomal races from northeast scotland were analyzed (2n = 32, 2n = 36, 2n = 40). within-race encounters resulted in similar average corticosterone levels in mice from the three races. for all three races, certain of the between-race encounters resulted in a significant incre ...19968724181
effects of chlordiazepoxide on maternal aggression in mice depend on experience of resident and sex of intruder.lactating mice respond differentially to intruders of differing sex, displaying defensive attack against the male and offensive attack against the female. such a phenotypic dichotomy appears to have adaptive value in that unfamiliar males pose a much greater threat to the offspring than do females. the present study examined the effects of the benzodiazepine anxiolytic chlordiazepoxide (cdp) (2.5-10.0 mg/kg) on this differential response pattern in aggression-naive (nonscreened) (ns) and aggress ...19968728555
a manipulative field experiment to examine the effect of capillaria hepatica (nematoda) on wild mouse populations in southern australia.a 12-month manipulative field study of the effect of a liver parasite, capillaria hepatica, on mouse populations (mus domesticus) was conducted in the mallee wheatlands of northwestern victoria. there were 2 untreated and 4 treated sites each consisting of a 16 km2 sampling zone. the parasite was released in september (spring) 1993 into an increasing mouse population which had a medium density (100-250 mice per ha). a third untreated site was monitored from january 1994. a simple but effective b ...19968773526
delayed expression of the insulin-like growth factor i (igf-i) gene in the xy sex-reversed female mouse ovary.when the y chromosome of mus musculus domesticus (ydom) mouse strain from tirano (italy) or mus musculus poschiavinus (ypos) from poschiavo (switzerland), is placed onto the c57bl/6j (b6) inbred background, the ydom chromosome fails to induce normal testicular differentiation and instead allows development of ovaries and ovotestes in embryonic life. during postnatal development some hermaphroditic males become fertile whereas the xy females lack normal estrus cyclicity, produce low levels of gon ...19968793618
genomic incompatibilities in the hybrid zone between house mice in denmark: evidence from steep and non-coincident chromosomal clines for robertsonian fusions.the pattern of chromosomal variation is investigated in house mice from the danish hybrid zone between the translocation-prone mus musculus domesticus and the chromosomally conservative m. m. musculus. the cytogenetic analysis confirmed the non-introgression of three pairs of robertsonian (rb) fusions from m. m. domesticus into the m. m. musculus genome. the geographic distribution of two of these rb fusions was shown to follow staggered chromosomal clines which increased in steepness the closer ...19968801185
competence of oocytes from the b6.ydom sex-reversed female mouse for maturation, fertilization, and embryonic development in vitro.when the y chromosome of a mus musculus domesticus mouse strain is placed onto the c57bl/6j (b6) inbred genetic background, the xy (b6.ydom) progeny develop ovaries or ovotestes, but not normal testes, during fetal life. at puberty, while some of the hermaphroditic males become fertile, none of the xy sex-reversed females produce litters. we have previously demonstrated that the eggs ovulated from the b6.ydom ovary undergo fertilization efficiently, but cannot develop beyond the 2-cell stage eit ...19968812128
an integrated genetic map of the pearl locus of mouse chromosome 13.we have used a mus domesticus/spretus congenic animal and two interspecific backcross panels to map genetically 30 sequence-tagged sites (stss) and 13 genes to the vicinity of the pearl locus on mouse chromosome 13. the stss defining the mapped region are from d13mit9 to d13mit37, spanning 10.6 cm. genes mapped to this region include versican (cspg2), gtpase activating protein (rasa), dihydrofolate reductase (dhfr), arylsulfatase (as-1), thrombin receptor (cf2r), hexosaminidase b(hexb), 3-hydrox ...19968828042
sex-determining genes on mouse autosomes identified by linkage analysis of c57bl/6j-ypos sex reversal.a powerful approach for identifying mammalian primary (gonadal) sex determination genes is the molecular genetic analyses of sex reversal conditions (that is, xx individuals with testicular tissue and xy individuals with ovarian tissue). here we determined the number and chromosomal location of autosomal and x-linked genes that cause sex reversal in c57bl/6j (b6) mice carrying a y chromosome of mus domesticus poschiavinus origin (ypos). b6 xypos mice develop either as females with exclusively ov ...19968841197
mus spretus line-1s in the mus musculus domesticus inbred strain c57bl/6j are from two different mus spretus line-1 subfamilies.a line-1 element, lic105, was found in the mus musculus domesticus inbred strain, c57bl/6j. upon sequencing, this element was found to belong to a m. spretus line-1 subfamily originating within the last 0.2 million years. this is the second spretus-specific line-1 subfamily found to be represented in c57bl/6j. although it is unclear how these m. spretus line-1s transferred from m. spretus to m. m. domesticus, it is now clear that at least two different spretus line-1 sequences have recently tran ...19968852852
evidence of hantavirus in wild rodents in northern ireland.a survey of evidence of rodent hantavirus infection in county down, northern ireland was carried out by using immunofluorescence to detect virus antigen and antibody. antibodies to hantavirus (r22 strain of seoul virus and hantaan 76-118) were found in 11/51 (21.6%) brown rats (rattus norvegicus), 1/31 (3.2%) field mice (apodemus sylvaticus) and 17/59 (28.8%) house mice (mus domesticus). seven rodents had evidence of hantavirus antigen in lung tissues. antibody positive animals were significantl ...19968870634
new polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13.we have used a mus domesticus/-mus spretus congenic animal that was selected for retention of mus spretus dna around the pearl locus to create a highly polymorphic region suitable for screening new markers. representation difference analysis (rda) was performed with either dna from the congenic animal or c57bl/6j as the driver for subtraction. four clones were identified, characterized, and converted to pcr-based polymorphic markers. three of the four markers equally subdivide a 10-cm interval c ...19968903722
evidence for the presence of different alpha-1-proteinase inhibitor genes products in mouse plasma.the plasma alpha-1-proteinase inhibitor (api) of three mouse species mus domesticus, m. caroli and m. pahori was isolated. each of the species isoforms were then separated by chromatofocusing; however, no significant differences in association rate constants toward human neutrophil elastase and bovine chymotrypsin were observed. the amino-acid sequence of the p'1-p'15 c-terminal fragments of the api variants indicate that mouse plasma contains at least two different active api isoforms in the ca ...19968922029
distortion of mendelian recovery ratio for a mouse hsr is caused by maternal and zygotic hsr in chromosome 1 which is found in many feral populations of mus musculus domesticus was shown in previous studies to consist of a high-copy long-range repeat cluster. one such cluster, mut, showed distorted transmission ratios when introduced by female parents. mut/+ offspring were preferentially recovered at the expense of +/+ embryos in the progeny of male mut/+ x female +/+ but were found at the expected 1:1 ratio in reciprocal crosses. preferential recovery of maternal mut was due to ...19968940901
segregation analysis of the testis-determining autosomal trait, tda, that differs between the c57bl/6j and dba/2j mouse strains suggests a multigenic threshold model.the testis-determining autosomal trait (tda) of the mouse was uncovered when the y chromosome of the poschiavinus variety of mus musculus domesticus was introduced into the c57bl/6j laboratory strain background. testis development is normal in the f1 generation but, in the backcross and subsequent crosses to c57bl/6j females, xy individuals with the poschiavinus y chromosome expressed bilateral ovaries or various combinations of an ovotestis with a contralateral ovary or testis or bilateral ovot ...19968984004
characterization and localization of the mprox1 gene directly upstream of the mouse alpha-globin gene cluster: identification of a polymorphic direct repeat in the 5'utr.the alpha-globin major regulatory element (alpha mre) positioned far upstream of the gene cluster is essential for the proper expression of the alpha-globin genes. analysis of the human and mouse alpha-globin upstream flanking regions (alpha ufr) has identified three nonglobin genes in the order dist1-mpg-prox1-alpha-globin. further characterization of the whole region indicates that the alpha mre and several other erythroid dnase hsss are associated with the transcription unit of the prox1 gene ...19968995756
genetic distinctiveness of a village population of house mice: relevance to speciation and chromosomal evolution.a population of house mice, mus musculus domesticus, from the village of migiondo was found to be genetically distinct from nearby populations in upper valtellina (italian alps). at the supernatant malic enzyme locus, mod1, the only alleles found in migiondo (c and n2) were virtually absent from the other populations in the valley, which were characterized by allele a. the extraordinary genetic distinctiveness of the migiondo population is apparently the result of genetic drift, perhaps coupled ...19979107050
sex and death in the mouse: genetically delayed reproduction and senescence.a mammalian model of genetically postponed aging would be an important tool to test not only different mechanisms of aging but also the predictive value of various biomarkers of the aging process. under conventional conditions, the historical strains of the laboratory mouse produce their first litter between 9 and 13 weeks of age and have a median time of death in their 2nd year. our posch-2 strain, which was derived from wild-caught mus musculus domesticus, produces its first litter in the curr ...19979134704
hippocampal morphology and open-field behavior in mus musculus domesticus and mus spretus inbred mice.mus spretus is extensively used in interspecific mouse backcross analyses employed to generate genetic linkage maps. however, little is known about its behavior and neuroanatomy, phenotypes for which large interstrain differences have been observed in mus musculus domesticus. behavioral and hippocampal neuroanatomical variables were measured in adult male mice from the inbred strains c57bl/6j (mus musculus domesticus) and seg (mus spretus). clear differences were found for behavioral responses t ...19979145545
the prevalence of cryptosporidium parvum and c. muris in mus domesticus, apodemus sylvaticus and clethrionomys glareolus in an agricultural system.wild mice and voles were tested for cryptosporidium during a 2-year survey at an agricultural site in warwickshire, united kingdom. c. parvum and c. muris, the two cryptosporidial species known to infect mammals, were detected. prevalence figures of 22%, 21% and 13% noted for c. parvum for mus domesticus, apodemus sylvaticus and clethrionomys glareolus, respectively, were higher than those recorded for c. muris at 10%, 6% and 2%. c. parvum causes the sometimes severe diarrhoeal disease cryptospo ...19979197396
the genetic structure of escherichia coli populations in feral house mice.escherichia coli was isolated from feral house mice (mus domesticus) during the course of a mouse plague in the state of victoria, australia. two farms were sampled over a period of 7 months and a total of 447 isolates were collected. the isolates were characterized using the techniques of randomly amplified polymorphic dna and multi-locus enzyme electrophoresis. the mean genetic diversity of this e. coli population (h = 0.24) was found to be substantially lower than the diversity of an e. col p ...19979202479
the gene encoding protein 4.2 is distinct from the mouse platelet storage pool deficiency mutation pallid.previous studies identified the gene encoding the erythrocyte membrane protein 4.2 (epb4.2) as a candidate for the mouse mutation pallid (pa); epb4.2 genetically colocalized near pa on mouse chromosome 2, and a truncated epb4.2 transcript was present in tissues derived from pallid mice. we report here evidence that epb4.2 and pa are not allelic. the pallid cdna and intron/exon boundaries show no significant variation from the known balb/c and c57bl/6j epb4.2 sequence, and normal immunoreactive 7 ...19979205130
the effect of early experience on mhc-based mate preferences in two b10.w strains of mice (mus domesticus).previous experiments have demonstrated that mice of some strains show mate preferences that are based on genes of the major histocompatibility complex (mhc), and rearing environment appears to influence these preferences. this experiment investigated if fostering affected mhc-based mate preferences in two additional strains of mice for which it was known that females exhibited mhc-dissimilar preferences. pups were exchanged between families of b10.gaa37 and b10.chr51 mouse strains, which differe ...19979210793
the sycp1 loci of the mouse genome: successive retropositions of a meiotic gene during the recent evolution of the genus.the murine sycp1 gene is expressed at the early stages of meiosis. we show that it is composed of a number of small exons and localized on mouse chromosome 3. in the laboratory strains, two retrogenes were also identified. the first one (sycp1-ps1), on chromosome 7, has accumulated point mutations and deletions and is not transcribed. a second retrogene (sycp1-ps2), on chromosome 8, is inserted within the continuity of a moderately repeated element, in an intron of another gene (cad11). the two ...19979286707
chromosomal transmission bias in laboratory hybrids between wild strains of the two european subspecies of house mice.laboratory crosses between wild strains of the two european house mouse subspecies mus musculus domesticus (2n = 34) and m. m. musculus (2n = 40) were performed to analyze the selective processes involved in the non-introgression of centromeric regions of robertsonian (rb) fusions in the danish hybrid zone. the chromosomal analysis of 226 backcross progeny from 22 reciprocal crosses showed that the segregation of the three rb fusions present did not significantly differ from mendelian expectatio ...19979383070
patterns of dna variability at x-linked loci in mus domesticus.introns of four x-linked genes (hprt, plp, glra2, and amg) were sequenced to provide an estimate of nucleotide diversity at nuclear genes within the house mouse and to test the neutral prediction that the ratio of intraspecific polymorphism to interspecific divergence is the same for different loci. hprt and plp lie in a region of the x chromosome that experiences relatively low recombination rates, while glra2 and amg lie near the telomere of the x chromosome, a region that experiences higher r ...19979383072
comparison of alkaline single cell gel (comet) and peripheral blood micronucleus assays in detecting dna damage caused by direct and indirect acting mutagens.the alkaline single cell gel (scg) or 'comet' and peripheral blood micronucleus (pbmn) assay have been used to compare the effects of the direct acting mutagens, methyl methanesulfonate (mms) and n-nitroso-n-methylurea (nmu), and the indirect acting mutagens, benzo[a]pyrene (bap), cyclophosphamide (cp) 9, 10-dimethyl-1,2-benzanthracene (dmba), and mitomycin c (mmc) in an inbred house mouse (mus domesticus) strain. the alkaline scg assay was able to detect dna damage from direct acting mutagens. ...19979403028
models for male infertility: the t haplotypes.the t haplotypes are variant alleles of genes in the proximal region of mouse chromosome 17, linked together by four inversions. while females carrying two t haplotypes are fertile, males are sterile. their spermatozoa exhibit severe motility defects and are unable to penetrate zona pellucida-free oocytes. spermatozoa from males carrying one t haplotype (t/+) exhibit mild motility deficits and a delay in penetration of the zona-free oocyte. the inversions of the t haplotypes contain several gene ...19979414479
a microsatellite-based mhc genotyping system for house mice (mus domesticus).major histocompatibility complex (mhc) genes are the most polymorphic loci known for vertebrates. although this has been known for over two decades, the selective forces maintaining this genetic diversity are unclear. efforts to study selection on these loci in nature have been hampered because no simple mhc typing systems are available. here, we describe and evaluate a microsatellite-based mhc genotyping system for house mice (mus domesticus). thirty-five mhc-linked microsatellite loci were tes ...19979420473
glucocorticoid response to forced exercise in laboratory house mice (mus domesticus).we examined the time course and sex differences of the glucocorticoid response to forced, moderate-intensity treadmill exercise in outbred laboratory house mice. mice (n = 64 total) were divided into eight groups, each of four males and four females, which were run on a motorized treadmill at 1.0 km/h for either 0, 2, 5, 10, 15, 25, 40, or 60 min. serum samples were taken immediately after exercise and corticosterone (cort) concentration was determined by radioimmunoassay. resting cort levels ra ...19989423970
hst7: a male sterility mutation perturbing sperm motility, flagellar assembly, and mitochondrial sheath differentiation.hst7, a mouse hybrid sterility locus, has been mapped in close linkage to four other hybrid sterility loci, on proximal chromosome 17 within the t complex. when an allele (s) of hst7 from the species mus spretus is crossed into the mus musculus domesticus (laboratory mouse) background, all male offspring are sterile. this occurs regardless of whether the hst7 allele on the other chromosome 17 homolog is wild-type (+) or an allele (t) derived from the structurally variant homolog known as a t hap ...19979432139
epoc and the energetics of brief locomotor activity in mus domesticus.excess post-exercise oxygen consumption (epoc) is normally not considered in determinations of the metabolic cost of activity. this approach overlooks an important energetic cost that an animal incurs as a result of activity. to examine the importance of epoc, we determined how the energetic cost of locomotion was affected by activity of short duration and high intensity. mice were run at maximum speed on a treadmill while enclosed in an open-flow respirometry system. after sprinting for 5, 15, ...19989433798
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