genetic analysis of liver tumorigenesis in sv40 t antigen transgenic mice implies a role for imprinted genes.liver tumors from interspecific hybrid, transgenic mice containing the sv40 early region linked to a mouse major urinary protein enhancer/promoter were analyzed for loss of heterozygosity to identify chromosomal regions which potentially contain genetic loci involved in multistep tumorigenesis. a broad pattern of complete and partial loss of heterozygosity or allelic imbalance was observed with frequent loss of heterozygosity/partial loss of heterozygosity of loci on chromosomes 1, 5, 7, 8, and ...19947987847
the hsr on chromosome 1 of the house mouse, mus domesticus: distribution and frequency in switzerland.a total of 357 house mice (mus domesticus) from 83 localities uniformly distributed throughout switzerland were screened for the presence of a homogenously staining region (hsr) on chromosome 1. altogether 47 mice from 11 localities were hsr/+ or hsr/hsr. one sample of 11 individuals all had an hsr/hsr karyotype. almost all mice with the variant were collected from the rhone valley (hsr frequency: 61%) and val bregaglia (hsr frequency: 81%). for samples from most of the area of switzerland, the ...19947813901
genetic control of retroviral disease in aging wild mice.different populations of wild mice (mus musculus domesticus) in los angeles and ventura counties were observed over their lifespan in captivity for expression of infectious murine leukemia virus (mulv) and murine mammary tumor virus (mmtv) and for the occurrence of cancer and other diseases. in most populations of feral mice these indigenous retroviruses were infrequently expressed and cancer seldom occurred until later in life (> 2 years old). mmtv was found in the milk of about 50% of wild mic ...19938125269
biochemical basis of warfarin and bromadiolone resistance in the house mouse, mus musculus domesticus.danish mice (mus musculus domesticus) genetically resistant to the anticoagulant action of two 4-hydroxycoumarins, warfarin and bromadiolone, were examined to determine their mechanism of resistance. the hepatic vitamin k epoxide reductase in the bromadiolone-resistant mice and in one phenotype of warfarin-resistant mice was highly insensitive to in vitro inhibition by warfarin and bromadiolone. the kinetic constants for the epoxide reductase from bromadiolone-resistant mice were also altered. t ...19948129744
phenotype and tcr gamma delta variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice (mus musculus domesticus): abundance of v gamma 1 transcripts and extensive delta gene order to study murine intestinal intraepithelial lymphocytes (iel) independent of factors imparted by conditions of laboratory housing and breeding, and to provide a basis for comparison of iel studies between inbred and outbred mouse populations, iel from the domestic house mouse, mus musculus domesticus, were analyzed by flow cytometric analyses using mabs to murine lymphocyte markers, and by polymerase chain reaction to study the tcr gamma and delta v gene repertoires. the majority of iel ...19948155599
polymorphism of the mouse transcription factor-encoding gene e2a.southern blotting analysis using a cdna probe consisting of the central portion of the e12 coding region has revealed two distinct forms of e2a, one which is common to all inbred and wild mouse strains derived from mus musculus musculus and mus musculus domesticus, whereas the other is less common and has only been found in the wild mouse population of mus musculus domesticus.19948163208
cloning and sequence analysis of the gene and cdna encoding mouse spermidine/spermine n1-acetyltransferase--a gene uniquely regulated by polyamines and their analogs.the polyamine catabolizing enzyme, spermidine/spermine n1-acetyltransferase (ssat), has been implicated as a critical determinant of polyamine pool maintenance. ssat has recently been shown to be positively regulated in human cell lines by polyamines and their analogs at the level of mrna accumulation. mouse la-4 lung adenoma cells treated with either spermine or the spermine analog, n1,n12-bis(ethyl)spermine, produced a 2.3 and 6.5-fold increase, respectively, in ssat mrna. prior evidence for t ...19938241266
new t-cell receptor gamma haplotypes in wild mice and evidence for limited tcrg-v gene polymorphism.tcrg gene polymorphism was investigated by southern blot analysis on a panel of laboratory and wild mouse strains using a set of probes which identify all known tcrg-v and -c genes. only three haplotypes are found in laboratory mice: ga, gb, and gc which are represented by balb/c, akr, and dba/2 prototypes respectively. ga and gc haplotypes are the most frequent among laboratory mice whereas gb is poorly represented. seven new haplotypes are described among 23 wild mice corresponding to four mus ...19938420824
population dynamics of aberrant chromosome 1 in mice.natural populations of two semispecies of house mouse, mus musculus domesticus and m.m. musculus, were found to be polymorphic for an aberrant chromosome 1 bearing a large inserted block of homogeneously staining heterochromatin. strong meiotic drive for the aberrant chromosome from m.m. musculus was previously observed in heterozygous female mice. there are at least three meiotic drive levels determined by different allelic variants of distorter. homozygotes had low viability and females showed ...19938314713
immunoglobulin constant kappa gene alleles in twelve strains of mice.genomic dna for the immunoglobulin (ig) constant kappa igk-c gene region was amplified by the polymerase chain reaction (pcr) method and sequenced from twelve commonly used inbred mouse strains. pcr products were used directly as templates in dideoxy-dna-sequencing, a method which avoids the sequencing errors caused by taq polymerase, since no cloning step is required. in restriction fragment length polymorphism (rflp) studies the sjl mouse strain has been shown to belong to a igk-c allogroup di ...19938436414
an ephemeral sex pheromone of female house mice (mus domesticus): pheromone fade-out time.male mice will emit ultrasounds to at least two chemosignals present in female mouse urine: a) an ephemeral unconditioned stimulus (ucs) present in freshly voided urine and b) a conditioned stimulus (cs) that remains after the ucs has disappeared. in this study, four phases examined the time frame over which the ucs disappeared. urine that had been aged for either 12, 15, 18, or 24 h was compared for vocalization elicitation with fresh urine (which contains the ucs) and metabolic cage-collected ...19938327598
mus spretus-specific line-1 dna probes applied to the cloning of the murine pearl locus.line-1 is the major family of long, interspersed, repetitive dna sequences found in mammalian genomes. the mouse species mus spretus contains large line-1 subfamilies that are distinguishable from the line-1 elements of laboratory mus domesticus strains by their content of particular nucleotide differences. oligonucleotides containing these differences act as m. spretus-specific line-1 hybridization probes. we have used these probes as a novel genetic tool in conjunction with an interspecific hy ...19938449493
characterizations of candidate genes for idd susceptibility from the diabetes-prone nod mouse strain.the nucleotide sequences of the nod and c57bl/6j alleles of glut-2, sod-2, and il-2 were determined by rt-pcr sequencing. each of these loci is located in intervals that strongly correlated with susceptibility to diabetes in an (nod/uf x c57bl/6j)f1 x nod/uf backcross. no significant variations in the alleles of glut-2 at 16 cm on chromosome (chr) 3 or sod-2 at 8 cm on chr 17 were detected. however, the il-2 allele in nod at 20 cm on chr 3 was found to differ from that in c57bl/6j by a complex m ...19938268651
olfactory discrimination of urinary odortypes from congenic strains (dba/1bg and dba1.c57bl10-ybg) of mice differing in their y chromosomes.differential effects of one or more genes in the nonpseudoautosomal region of the dba1 and c57bl10 y chromosomes on offensive attack may be mediated, at least in part, by differential effects of this y chromosomal gene(s) on the sending and receiving of discriminable urinary odortypes. this hypothesis is based, in part, on the report that urine from a pair of y chromosomal congenic strains (c57bl6.akr-y and c57bl6) of mice can be discriminated in a y maze test. the akr and c57bl6 y chromosomes c ...19938352719
prevalence of viral antibodies and helminths in field populations of house mice (mus domesticus) in southeastern australia.a 13-month study of wild mice (mus domesticus) in wheatlands in southeastern australia contrasted changes in the seroprevalence of antibody to 13 viruses and the occurrence of helminths with changes in their population dynamics. mice were seropositive for mouse hepatitis virus (mhv), rotavirus, minute virus of mice (mvm), mouse adenovirus (madv), reovirus (reo 3), and murine cytomegalovirus (mcmv). the seroprevalences of all but rotavirus varied significantly with time and increased with host de ...19938472782
sex reversal in c57bl/6j-ypos mice corrected by a sry transgene.c57bl/6j mice carrying a mus domesticus poschiavinus y chromosome (ypos) develop as females with ovarian tissue or as hermaphrodites with ovarian and testicular tissue. we tested the hypothesis that the y-linked component of this inherited sex reversal is caused by the m. d. poschiavinus y-linked testis determining gene (symbolized tdy or sry) by examining gonadal development in c57bl/6j xypos mice carrying a m. musculus allele of sry as a transgene. we found that in the presence of the transgen ...19958570690
association of xmv-10 and the non-agouti (a) mutation explained by close linkage instead of a previous survey of endogenous proviruses among inbred mouse strains, the xmv-10 provirus was found only in strains that carried the non-agouti (a) mutation (frankel et al. j. virol. 63: 1763-1774, 1989). to determine whether insertion of xmv-10 caused the a mutation, we cloned a portion of xmv-10 and its insertion site. using a fragment of flanking cellular dna as a southern hybridization probe, we found that the xmv-10 provirus was still present in revertant alleles of a to a(t) or aw. a r ...19948111126
sex differences in plasma corticosterone in mouse fetuses are mediated by differential placental transport from the mother and eliminated by maternal adrenalectomy or stress.the effect of changes in maternal corticosterone concentrations, induced by maternal stress, maternal adrenalectomy or both, on concentration of corticosterone in serum and in adrenals of mouse (mus domesticus) fetuses was examined. higher baseline serum corticosterone concentrations were found in female fetuses than in male fetuses; however, there was no sex difference in the content of corticosterone in adrenals collected from these fetuses. sex differences were observed in the fetal response ...19938107008
targeted cloning of a subfamily of line-1 elements by subfamily-specific line-1-pcr.subfamily-specific line-1 pcr (ssl1-pcr) is the targeted amplification and cloning of defined subfamilies of line-1 elements and their flanking sequences. the targeting is accomplished by incorporating a subfamily-specific sequence difference at the 3' end of a line-1 pcr primer and pairing it with a primer to an anchor ligated within the flanking region. ssl1-pcr was demonstrated by targeting amplification of a mus spretus-specific line-1 subfamily. the amplified fragments were cloned to make a ...19938499652
further examination of the kinetics of gonadal development in xy female mice.y-chromosomes derived from house mouse populations of mus domesticus are able to lead to the formation of xy females (i.e. hermaphrodite individuals with both ovarian and testicular tissues) when inserted, by appropriate crosses, into the c57bl/6j genome, which usually carries the mus musculus y chromosome. an abnormal interaction between the testis-determining gene on the y (of domesticus origin) and an autosomal recessive allele (of musculus origin) needed for testicular formation is the likel ...19938374503
directional dominance and a developmental model for the expression of the tda testis-determining autosomal trait of the mouse.the posch-2 y chromosome from the poschiavinus variety of mus musculus domesticus causes incomplete testis development in the recessive autosomal background of the c57bl/6j laboratory mouse strain. testis development is normal with the posch-2 y in its native strain background as well as in some strains of the laboratory mouse such as dba/2j. the phenotype or expression of xy gonadal hermaphroditism in a c57bl/6j strain, which was constructed to be consomic for the posch-2 y, is a threshold trai ...19968675000
a manipulative field experiment to examine the effect of capillaria hepatica (nematoda) on wild mouse populations in southern australia.a 12-month manipulative field study of the effect of a liver parasite, capillaria hepatica, on mouse populations (mus domesticus) was conducted in the mallee wheatlands of northwestern victoria. there were 2 untreated and 4 treated sites each consisting of a 16 km2 sampling zone. the parasite was released in september (spring) 1993 into an increasing mouse population which had a medium density (100-250 mice per ha). a third untreated site was monitored from january 1994. a simple but effective b ...19968773526
high-resolution comparative mapping of mouse chromosome 17.we have constructed a high-resolution genetic linkage map spanning the proximal 32 cm of mouse chromosome 17 including the t complex. simple sequence repeats at d17tu1 and d17mit6 were employed to identify 121 recombinants among 374 offspring of a (c57bl/6 x cast/ei) x c57bl/6 backcross. in contrast to previously reported interspecific mus domesticus x mus spretus backcrosses, we did not observe inversion polymorphisms with our cross. this and the relatively high frequency of dna polymorphisms b ...19938406442
absence of correlation between sry polymorphisms and xy sex reversal caused by the m. m. domesticus y chromosome.mus musculus domesticus y chromosomes (ydom chrs) vary in their ability to induce testes in the strain c57bl/6j. in severe cases, xy females develop (xydom sex reversal). to identify the molecular basis for the sex reversal, a 2.7-kb region of sry, the testis-determining gene, was sequenced from ydom chrs linked to normal testis determination, transient sex reversal, and severe sex reversal. four mutations were identified. however, no correlation exists between these mutations and severity of xy ...19968617507
region-specific rates of molecular evolution: a fourfold reduction in the rate of accumulation of "silent" mutations in transcribed versus nontranscribed regions of homologous dna fragments derived from two closely related mouse species.we have sequenced homologous dna fragments of 2.7 and 2.8 kbp derived from the closely related mouse species mus musculus domesticus (m. domesticus) and mus musculus musculus (m. musculus), respectively. these two species diverged approximately 1 million years ago. each dna fragment contains 1.35 kbp of the 3' end of the constitutively expressed 2.2-kbp aprt (adenine phosphoribosyltransferase) gene and a similarly sized nontranscribed region downstream of the aprt gene. the aprt gene region cont ...19938433377
endocrine differentiation of the xy sex-reversed mouse ovary during postnatal development.when the mouse y chromosome of mus musculus domesticus is placed onto the c57bl/6j genetic background, half of the xy progeny develop bilateral ovaries and the female phenotype, but lack regular estrous cyclicity and lose embryos after fertilization. in the present study, we compared the endocrinological activity of xy ovaries with xx ovaries during postnatal development by measuring steroids in the incubation medium by radioimmunoassay. at 1 day postpartum (d.p.p.), production of progesterone a ...19938499334
experimental evidence of genetic determinism in high susceptibility to intestinal pinworm infection in mice: a hybrid zone the hybrid zone of the two mouse subspecies mus musculus musculus and mus musculus domesticus, mice with hybrid genotypes harbour, on the average, more helminth parasites (cestodes and nematodes) than mice of the two parental taxa. in order to determine the roles played by genetic parameters in this phenomenon, mice with recombined and parental genotypes were experimentally infected with the intestinal pinworm aspiculuris tetraptera, a natural parasite of the house mouse. the results showed t ...19938316436
a b2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene.a variety of cdna and genomic clones for the gamma-subunit of mouse muscle phosphorylase kinase (phk-gamma m) have been isolated and characterized. the murine gene for phk-gamma m (phkg) exhibits multiple transcription start sites that are identical in skeletal muscle, cardiac muscle, and brain. the gene is composed of 10 exons and includes a 4.9-kb intron located in the 5' untranslated region. two mrna species of 1.75 and 2.55 kb are produced from phkg in icr and c57bl/10 mice; these transcript ...19938486349
a serologic survey for viruses and mycoplasma pulmonis among wild house mice (mus domesticus) in southeastern australia.plasma samples from 267 wild house mice (mus domesticus) trapped at 14 sites in southeastern australia were screened for antibody to 14 viruses normally associated with laboratory-reared rodents and to mycoplasma pulmonis. serologic prevalence was high for murine cytomegalovirus (99%, n = 94), murine coronavirus (95%), and murine rotavirus (74%). samples from mice collected at all sites contained antibody to these viruses. the serologic prevalence was lower for mouse adenovirus, strain k87 (37%) ...19938487371
loss of telomeric sites in the chromosomes of mus musculus domesticus (rodentia: muridae) during robertsonian rearrangements.mouse chromosomes possessing multiple robertsonian rearrangements (rb chromosomes) have been examined using fluorescence in situ hybridization with the telomeric consensus sequence (ttaggg)n. no hybridization signals were detected at the primary constriction of rb chromosomes. this observation leads us to conclude that the formation of rb chromosomes in the mouse is invariably associated with the loss of telomeric regions. more significantly, a further alteration in regions flanking the primary ...19958528584
identification and characterization of a novel conserved dna repeat.homozygous in(10)17rk mice contain a paracentric inversion within chromosome (chr) 10 and exhibit a pygmy phenotype, suggesting that the distal inversion breakpoint is within the pygmy (pg) locus. in order to obtain the pygmy gene by positional cloning procedures, in(10)17rk dna was subjected to rflp analysis with single-copy probes derived from the wild-type pygmy locus. this analysis identified a dna polymorphism in the dba/2j mouse strain on which the in(10)17rk mutation was originally induce ...19958535068
an ige-binding tame esterase in the urine of the mouse, mus musculus domesticus.among the highly heterogeneous components of the mouse urinary protein complex (mup) a hydrolase was detected which was capable of spliting the proteinase ester substrate tame as well as a synthetic chromogenic tripeptide specific for tissue and urinary kallikreins. the binding of mouse-specific ige antibodies from the serum of a highly mouse-allergic patient occurred preferentially to this kallikrein-like enzyme. this finding underscores the possible association of significant biological activi ...19958537097
mus spretus line-1s in the mus musculus domesticus inbred strain c57bl/6j are from two different mus spretus line-1 subfamilies.a line-1 element, lic105, was found in the mus musculus domesticus inbred strain, c57bl/6j. upon sequencing, this element was found to belong to a m. spretus line-1 subfamily originating within the last 0.2 million years. this is the second spretus-specific line-1 subfamily found to be represented in c57bl/6j. although it is unclear how these m. spretus line-1s transferred from m. spretus to m. m. domesticus, it is now clear that at least two different spretus line-1 sequences have recently tran ...19968852852
distortion of mendelian recovery ratio for a mouse hsr is caused by maternal and zygotic hsr in chromosome 1 which is found in many feral populations of mus musculus domesticus was shown in previous studies to consist of a high-copy long-range repeat cluster. one such cluster, mut, showed distorted transmission ratios when introduced by female parents. mut/+ offspring were preferentially recovered at the expense of +/+ embryos in the progeny of male mut/+ x female +/+ but were found at the expected 1:1 ratio in reciprocal crosses. preferential recovery of maternal mut was due to ...19968940901
the genetic basis of murine and human sex determination: a review.determination of mammalian sex depends on the presence or absence of a functional testis. testes are determined by the activity of the testis determining factor encoded by the sex determining gene, y (sry) located on the y chromosome. considerable evidence suggests that the sry gene is the only gene on the y chromosome that is both necessary and sufficient to initiate testis determination. other steps in the mammalian sex determining pathway are unknown, although recent advances have shown that ...19958575930
h-2 polymorphisms are more uniformly distributed than allozyme polymorphisms in natural populations of house mice.patterns of h-2 and allozyme polymorphism in natural populations of house mice from europe, north africa and south america were analyzed. the purpose of the analysis was to determine whether h-2 and allozyme polymorphisms were similarly distributed both geographically and temporally in wild mice. two subspecies of house mice, mus musculus domesticus and m. m. musculus were sampled and the polymorphisms of two h-2 class i genes, h-2k and h-2d, and 34 allozyme-encoding genes were surveyed. the thr ...19888608923
functional comparison of the mus musculus molossinus and mus musculus domesticus sry genes.the sry gene functions as a genetic switch initiating testicular development of the indifferent mammalian gonad. the mus musculus molossinus sry open reading frame (orf) encodes a 395-amino acid transcription factor (msry) that specifically binds and bends dna through its n-terminal hmg domain and activates transcription through its long c-terminal (residues 144-366) glutamine/histidine-rich activation domain. the m. m. domesticus sry orf encodes a highly homologous, truncated protein (dsry) of ...19958614401
corticosterone and interchromosomal race discrimination in the house mouse.we report results of a study on emotional arousal, as reflected by corticosterone variation, following dyadic encounters within and between chromosomal races of the house mouse (mus musculus domesticus). three contiguous chromosomal races from northeast scotland were analyzed (2n = 32, 2n = 36, 2n = 40). within-race encounters resulted in similar average corticosterone levels in mice from the three races. for all three races, certain of the between-race encounters resulted in a significant incre ...19968724181
effects of chlordiazepoxide on maternal aggression in mice depend on experience of resident and sex of intruder.lactating mice respond differentially to intruders of differing sex, displaying defensive attack against the male and offensive attack against the female. such a phenotypic dichotomy appears to have adaptive value in that unfamiliar males pose a much greater threat to the offspring than do females. the present study examined the effects of the benzodiazepine anxiolytic chlordiazepoxide (cdp) (2.5-10.0 mg/kg) on this differential response pattern in aggression-naive (nonscreened) (ns) and aggress ...19968728555
genetic distinctiveness of a village population of house mice: relevance to speciation and chromosomal evolution.a population of house mice, mus musculus domesticus, from the village of migiondo was found to be genetically distinct from nearby populations in upper valtellina (italian alps). at the supernatant malic enzyme locus, mod1, the only alleles found in migiondo (c and n2) were virtually absent from the other populations in the valley, which were characterized by allele a. the extraordinary genetic distinctiveness of the migiondo population is apparently the result of genetic drift, perhaps coupled ...19979107050
genomic incompatibilities in the hybrid zone between house mice in denmark: evidence from steep and non-coincident chromosomal clines for robertsonian fusions.the pattern of chromosomal variation is investigated in house mice from the danish hybrid zone between the translocation-prone mus musculus domesticus and the chromosomally conservative m. m. musculus. the cytogenetic analysis confirmed the non-introgression of three pairs of robertsonian (rb) fusions from m. m. domesticus into the m. m. musculus genome. the geographic distribution of two of these rb fusions was shown to follow staggered chromosomal clines which increased in steepness the closer ...19968801185
evaluation of a nematode (capillaria hepatica bancroft, 1893) as a control agent for populations of house mice (mus musculus domesticus schwartz and schwartz, 1943).sudden, large-scale infestations of house mice (mus musculus domesticus) occur irregularly in the cereal-growing regions of australia, resulting in substantial economic losses. mathematical modelling has been used to evaluate the use of the liver nematode capillaria hepatica as a potential agent for the biological control of mouse populations. the models suggest that c. hepatica is unlikely to be successful as a single-release control agent: instead, the parasite would need to be released when i ...19938518449
delayed expression of the insulin-like growth factor i (igf-i) gene in the xy sex-reversed female mouse ovary.when the y chromosome of mus musculus domesticus (ydom) mouse strain from tirano (italy) or mus musculus poschiavinus (ypos) from poschiavo (switzerland), is placed onto the c57bl/6j (b6) inbred background, the ydom chromosome fails to induce normal testicular differentiation and instead allows development of ovaries and ovotestes in embryonic life. during postnatal development some hermaphroditic males become fertile whereas the xy females lack normal estrus cyclicity, produce low levels of gon ...19968793618
competence of oocytes from the b6.ydom sex-reversed female mouse for maturation, fertilization, and embryonic development in vitro.when the y chromosome of a mus musculus domesticus mouse strain is placed onto the c57bl/6j (b6) inbred genetic background, the xy (b6.ydom) progeny develop ovaries or ovotestes, but not normal testes, during fetal life. at puberty, while some of the hermaphroditic males become fertile, none of the xy sex-reversed females produce litters. we have previously demonstrated that the eggs ovulated from the b6.ydom ovary undergo fertilization efficiently, but cannot develop beyond the 2-cell stage eit ...19968812128
patterns of dna variability at x-linked loci in mus domesticus.introns of four x-linked genes (hprt, plp, glra2, and amg) were sequenced to provide an estimate of nucleotide diversity at nuclear genes within the house mouse and to test the neutral prediction that the ratio of intraspecific polymorphism to interspecific divergence is the same for different loci. hprt and plp lie in a region of the x chromosome that experiences relatively low recombination rates, while glra2 and amg lie near the telomere of the x chromosome, a region that experiences higher r ...19979383072
evidence of hantavirus in wild rodents in northern ireland.a survey of evidence of rodent hantavirus infection in county down, northern ireland was carried out by using immunofluorescence to detect virus antigen and antibody. antibodies to hantavirus (r22 strain of seoul virus and hantaan 76-118) were found in 11/51 (21.6%) brown rats (rattus norvegicus), 1/31 (3.2%) field mice (apodemus sylvaticus) and 17/59 (28.8%) house mice (mus domesticus). seven rodents had evidence of hantavirus antigen in lung tissues. antibody positive animals were significantl ...19968870634
chromosomal transmission bias in laboratory hybrids between wild strains of the two european subspecies of house mice.laboratory crosses between wild strains of the two european house mouse subspecies mus musculus domesticus (2n = 34) and m. m. musculus (2n = 40) were performed to analyze the selective processes involved in the non-introgression of centromeric regions of robertsonian (rb) fusions in the danish hybrid zone. the chromosomal analysis of 226 backcross progeny from 22 reciprocal crosses showed that the segregation of the three rb fusions present did not significantly differ from mendelian expectatio ...19979383070
new polymorphic markers in the vicinity of the pearl locus on mouse chromosome 13.we have used a mus domesticus/-mus spretus congenic animal that was selected for retention of mus spretus dna around the pearl locus to create a highly polymorphic region suitable for screening new markers. representation difference analysis (rda) was performed with either dna from the congenic animal or c57bl/6j as the driver for subtraction. four clones were identified, characterized, and converted to pcr-based polymorphic markers. three of the four markers equally subdivide a 10-cm interval c ...19968903722
segregation analysis of the testis-determining autosomal trait, tda, that differs between the c57bl/6j and dba/2j mouse strains suggests a multigenic threshold model.the testis-determining autosomal trait (tda) of the mouse was uncovered when the y chromosome of the poschiavinus variety of mus musculus domesticus was introduced into the c57bl/6j laboratory strain background. testis development is normal in the f1 generation but, in the backcross and subsequent crosses to c57bl/6j females, xy individuals with the poschiavinus y chromosome expressed bilateral ovaries or various combinations of an ovotestis with a contralateral ovary or testis or bilateral ovot ...19968984004
evidence for the presence of different alpha-1-proteinase inhibitor genes products in mouse plasma.the plasma alpha-1-proteinase inhibitor (api) of three mouse species mus domesticus, m. caroli and m. pahori was isolated. each of the species isoforms were then separated by chromatofocusing; however, no significant differences in association rate constants toward human neutrophil elastase and bovine chymotrypsin were observed. the amino-acid sequence of the p'1-p'15 c-terminal fragments of the api variants indicate that mouse plasma contains at least two different active api isoforms in the ca ...19968922029
characterization and localization of the mprox1 gene directly upstream of the mouse alpha-globin gene cluster: identification of a polymorphic direct repeat in the 5'utr.the alpha-globin major regulatory element (alpha mre) positioned far upstream of the gene cluster is essential for the proper expression of the alpha-globin genes. analysis of the human and mouse alpha-globin upstream flanking regions (alpha ufr) has identified three nonglobin genes in the order dist1-mpg-prox1-alpha-globin. further characterization of the whole region indicates that the alpha mre and several other erythroid dnase hsss are associated with the transcription unit of the prox1 gene ...19968995756
sex and death in the mouse: genetically delayed reproduction and senescence.a mammalian model of genetically postponed aging would be an important tool to test not only different mechanisms of aging but also the predictive value of various biomarkers of the aging process. under conventional conditions, the historical strains of the laboratory mouse produce their first litter between 9 and 13 weeks of age and have a median time of death in their 2nd year. our posch-2 strain, which was derived from wild-caught mus musculus domesticus, produces its first litter in the curr ...19979134704
an integrated genetic map of the pearl locus of mouse chromosome 13.we have used a mus domesticus/spretus congenic animal and two interspecific backcross panels to map genetically 30 sequence-tagged sites (stss) and 13 genes to the vicinity of the pearl locus on mouse chromosome 13. the stss defining the mapped region are from d13mit9 to d13mit37, spanning 10.6 cm. genes mapped to this region include versican (cspg2), gtpase activating protein (rasa), dihydrofolate reductase (dhfr), arylsulfatase (as-1), thrombin receptor (cf2r), hexosaminidase b(hexb), 3-hydrox ...19968828042
the gene encoding protein 4.2 is distinct from the mouse platelet storage pool deficiency mutation pallid.previous studies identified the gene encoding the erythrocyte membrane protein 4.2 (epb4.2) as a candidate for the mouse mutation pallid (pa); epb4.2 genetically colocalized near pa on mouse chromosome 2, and a truncated epb4.2 transcript was present in tissues derived from pallid mice. we report here evidence that epb4.2 and pa are not allelic. the pallid cdna and intron/exon boundaries show no significant variation from the known balb/c and c57bl/6j epb4.2 sequence, and normal immunoreactive 7 ...19979205130
the sycp1 loci of the mouse genome: successive retropositions of a meiotic gene during the recent evolution of the genus.the murine sycp1 gene is expressed at the early stages of meiosis. we show that it is composed of a number of small exons and localized on mouse chromosome 3. in the laboratory strains, two retrogenes were also identified. the first one (sycp1-ps1), on chromosome 7, has accumulated point mutations and deletions and is not transcribed. a second retrogene (sycp1-ps2), on chromosome 8, is inserted within the continuity of a moderately repeated element, in an intron of another gene (cad11). the two ...19979286707
sex-determining genes on mouse autosomes identified by linkage analysis of c57bl/6j-ypos sex reversal.a powerful approach for identifying mammalian primary (gonadal) sex determination genes is the molecular genetic analyses of sex reversal conditions (that is, xx individuals with testicular tissue and xy individuals with ovarian tissue). here we determined the number and chromosomal location of autosomal and x-linked genes that cause sex reversal in c57bl/6j (b6) mice carrying a y chromosome of mus domesticus poschiavinus origin (ypos). b6 xypos mice develop either as females with exclusively ov ...19968841197
pregnancy block in house mice (mus domesticus) as a function of t-complex genotype: examination of the mate choice and male infanticide hypotheses.pregnancy block, whereby recently mated female mice abort their pregnancies when exposed to novel (strange) males, was studied in house mice (mus domesticus) differing in t-complex genotype; t-mutations are deleterious and +/t females avoid +/t males as mates. the results of experiment 1, in which the genotype of the female, stud male, and strange male was systematically varied, showed that pregnancy block was most frequent when the strange male was +/+. because this effect was not enhanced amon ...19989528117
a description of the ontogeny of mouse agonistic behavior.the development of agonistic behavior was characterized in outbred swiss cd-1 male mus domesticus. at weaning (postnatal day [pnd] 21), mice were housed either individually or as male pairs. social encounters were carried out between dyads of initially unfamiliar same-age and same-housing subjects every 3rd day, from pnd 23 to 47. the majority of both offensive and defensive elements had their onset around pnd 29. overall, their expression increased around puberty (i.e., on pnd 35), which also r ...19989528111
the effect of early experience on mhc-based mate preferences in two b10.w strains of mice (mus domesticus).previous experiments have demonstrated that mice of some strains show mate preferences that are based on genes of the major histocompatibility complex (mhc), and rearing environment appears to influence these preferences. this experiment investigated if fostering affected mhc-based mate preferences in two additional strains of mice for which it was known that females exhibited mhc-dissimilar preferences. pups were exchanged between families of b10.gaa37 and b10.chr51 mouse strains, which differe ...19979210793
decreased physical performance of congenic mice with mismatch between the nuclear and the mitochondrial genome.maternal transmission of mitochondrial dna (mtdna) allows us to generate mtdna congenic strain by repeating backcrosses of female mice to male mice of an inbred strain, which carries different mtdna haplotype from that of the female progenitor. since genetic backgrounds of inbred strains commonly used (e.g., c57bl/6j [b6] and balb/c) are mainly derived from an european subspecies of mus musculus domesticus, congenic strains, in which mtdna originated from an asian subspecies m. musculus musculus ...19989546205
lymphocyte proliferative response in brown bears: cytokine role and glucocorticoid effect.lymphocyte stimulation and proliferation play a pivotal role in the immune response to soluble as well as to cellular, bacterial, and viral antigens. in this study, peripheral blood mononuclear cells (pbmc), mainly composed of lymphocytes, were separated by ficoll-hypaque density gradient centrifugation from 50-ml jugular vein blood samples drawn from six captive and five wild-caught brown bears (ursus arctos) (eight apennine brown bears from the italian population; three of undetermined origin) ...19989542157
comparison of alkaline single cell gel (comet) and peripheral blood micronucleus assays in detecting dna damage caused by direct and indirect acting mutagens.the alkaline single cell gel (scg) or 'comet' and peripheral blood micronucleus (pbmn) assay have been used to compare the effects of the direct acting mutagens, methyl methanesulfonate (mms) and n-nitroso-n-methylurea (nmu), and the indirect acting mutagens, benzo[a]pyrene (bap), cyclophosphamide (cp) 9, 10-dimethyl-1,2-benzanthracene (dmba), and mitomycin c (mmc) in an inbred house mouse (mus domesticus) strain. the alkaline scg assay was able to detect dna damage from direct acting mutagens. ...19979403028
models for male infertility: the t haplotypes.the t haplotypes are variant alleles of genes in the proximal region of mouse chromosome 17, linked together by four inversions. while females carrying two t haplotypes are fertile, males are sterile. their spermatozoa exhibit severe motility defects and are unable to penetrate zona pellucida-free oocytes. spermatozoa from males carrying one t haplotype (t/+) exhibit mild motility deficits and a delay in penetration of the zona-free oocyte. the inversions of the t haplotypes contain several gene ...19979414479
hst7: a male sterility mutation perturbing sperm motility, flagellar assembly, and mitochondrial sheath differentiation.hst7, a mouse hybrid sterility locus, has been mapped in close linkage to four other hybrid sterility loci, on proximal chromosome 17 within the t complex. when an allele (s) of hst7 from the species mus spretus is crossed into the mus musculus domesticus (laboratory mouse) background, all male offspring are sterile. this occurs regardless of whether the hst7 allele on the other chromosome 17 homolog is wild-type (+) or an allele (t) derived from the structurally variant homolog known as a t hap ...19979432139
epoc and the energetics of brief locomotor activity in mus domesticus.excess post-exercise oxygen consumption (epoc) is normally not considered in determinations of the metabolic cost of activity. this approach overlooks an important energetic cost that an animal incurs as a result of activity. to examine the importance of epoc, we determined how the energetic cost of locomotion was affected by activity of short duration and high intensity. mice were run at maximum speed on a treadmill while enclosed in an open-flow respirometry system. after sprinting for 5, 15, ...19989433798
evidence for cyclophosphamide-induced gene conversion and mutation in mouse germ cells.cyclophosphamide (cp) is a widely used antineoplastic drug. it tests positive in several genotoxicity assays, including those with endpoints such as chromosomal aberrations in mammalian cells, mitotic recombination in drosophila melanogaster, and dominant lethal mutations in rodents. we have explored the effects of cp on genome stability of mouse (mus domesticus) spermatogenic cells, using a recombination-based transgenic assay called muscateer. in this system, intrachromosomal gene conversion e ...19979439729
population genetic structure in a robertsonian race of house mice: evidence from microsatellite polymorphism.genetic evidence was assessed for inbreeding and population subdivision in a robertsonian fusion (rb) race of the western european form of house mouse, mus musculus domesticus, in central belgium. inbreeding, and the factors responsible for subdivision (genetic drift and extinction-recolonization) can theoretically influence the fixation of underdominant rb variants. the data consisted of allele frequencies of eight microsatellite loci and of the rb(4.12) and rb(5.10) chromosomes. six population ...19989474776
hippocampal morphology and open-field behavior in mus musculus domesticus and mus spretus inbred mice.mus spretus is extensively used in interspecific mouse backcross analyses employed to generate genetic linkage maps. however, little is known about its behavior and neuroanatomy, phenotypes for which large interstrain differences have been observed in mus musculus domesticus. behavioral and hippocampal neuroanatomical variables were measured in adult male mice from the inbred strains c57bl/6j (mus musculus domesticus) and seg (mus spretus). clear differences were found for behavioral responses t ...19979145545
live-borns from xx but not xy oocytes in the chimeric mouse ovary composed of b6.y(tir) and xx cells.when the y chromosome of some mus musculus domesticus subspecies is placed onto a c57bl/6j mouse background, the xy (b6.y(tir)) progeny develop only ovaries or ovotestes during fetal life. the xy sex-reversed female is infertile mainly because of death of embryos during preimplantation development. in the present study, we constructed female mouse chimera composed of b6.y(tir) and xx balb/c cells to determine whether developmental incompetence of xy oocytes can be attributed to defects in the oo ...19989475416
the prevalence of cryptosporidium parvum and c. muris in mus domesticus, apodemus sylvaticus and clethrionomys glareolus in an agricultural system.wild mice and voles were tested for cryptosporidium during a 2-year survey at an agricultural site in warwickshire, united kingdom. c. parvum and c. muris, the two cryptosporidial species known to infect mammals, were detected. prevalence figures of 22%, 21% and 13% noted for c. parvum for mus domesticus, apodemus sylvaticus and clethrionomys glareolus, respectively, were higher than those recorded for c. muris at 10%, 6% and 2%. c. parvum causes the sometimes severe diarrhoeal disease cryptospo ...19979197396
the genetic structure of escherichia coli populations in feral house mice.escherichia coli was isolated from feral house mice (mus domesticus) during the course of a mouse plague in the state of victoria, australia. two farms were sampled over a period of 7 months and a total of 447 isolates were collected. the isolates were characterized using the techniques of randomly amplified polymorphic dna and multi-locus enzyme electrophoresis. the mean genetic diversity of this e. coli population (h = 0.24) was found to be substantially lower than the diversity of an e. col p ...19979202479
[natural hybridization between two subspecies of the house mouse, mus musculus domesticus and mus musculus castaneus, near lake casitas, california].the house mouse mus musculus is a polytypic species, distributed worldwide, with three main subspecies: m. m. musculus in the north-east of eurasia, m. m. castaneus in south-east asia, and m. m. domesticus in europe, the near-east, and africa. this last subspecies may also be found in australia and the americas, where it was brought by european colonization. previous studies, however, have shown the presence of specific antiviral determinants of asian origin in a mouse population at lake casitas ...19989549063
neuronal and behavioral differences between mus musculus domesticus (c57bl/6jby) and mus musculus castaneus (cast/ei).previous studies have demonstrated that classical inbred strains of laboratory mice do not exhibit large genetic distances when simple sequence repeats (ssrs) are used to test for their polymorphisms whereas mice from wild origin exhibit high polymorphisms (more than 90%) for these sequence when compared with classical inbred strains of laboratory mice. the difference between mus musculus castaneus and c57bl/6j reaches 98% and f1s male and female are fertile. these two properties pave the way fo ...19989754885
olfactory preferences in two strains of wild mice, mus musculus musculus and mus musculus domesticus, and their hybrids.we studied olfactory preferences of two strains of mice, mus musculus musculus and mus musculus domesticus (considered here to be subspecies), and their hybrids, to examine the possible role of odours as a behavioural, premating mechanism that could explain the characteristics of their natural hybrid zone. we used a choice test with the bedding material of animals of the opposite sex from the animal tested and from both subspecies. male and female m. m. domesticus showed no preference either for ...19989787027
prenatal and postnatal maternal effects on body weight in cross-fostering experiment on two subspecies of mice.a cross-fostering experiment was conducted on two quite distinct subspecies of mice, domesticated laboratory mouse of cf#1 (mus musculus domesticus) and yonakuni wild mouse (yk, mus musculus molossinus yonakuni), to estimate the prenatal and postnatal maternal effects on body weight of offspring. mating was done between subspecies, two or three females being mated to a male at nine-ten weeks of age. two dams of different subspecies that littered at the same day were used as a group of foster dam ...19989606419
chromosomal heterozygosity and fertility in house mice (mus musculus domesticus) from northern italy.following the discovery of over 40 robertsonian (rb) races of mus musculus domesticus in europe and north africa, the house mouse has been studied extensively as an ideal model to determine the chromosomal changes that may cause or accompany speciation. current models of chromosomal speciation are based on the assumption that heterozygous individuals have a particularly low fertility, although recent studies indicate otherwise. despite their importance, fertility estimates for the house mouse ar ...19989799266
ecg of the "newborn" mouse (mus domesticus) with specific reference to comparative av transmission.the objective of this study was to record the ecg of the smallest living mammal to extend the domain of data for comparative av nodal electrophysiologic purposes. these data are needed to establish the relationship between the pr interval and heart size in mammalian species of all sizes.199910090220
the cryptosporidium "mouse" genotype is conserved across geographic areas.a 298-bp region of the cryptosporidium parvum 18s rrna gene and a 390-bp region of the acetyl coenzyme a synthetase gene were sequenced for a range of cryptosporidium isolates from wild house mice (mus domesticus), a bat (myotus adversus), and cattle from different geographical areas. previous research has identified a distinct genotype, referred to as the "mouse"-derived cryptosporidium genotype, common to isolates from australian mice. comparison of a wider range of australian mouse isolates w ...199910203475
evolution of a mammalian promoter through changes in patterns of transcription factor binding.previously, we identified evolutionarily derived changes in the pattern of nuclear factor binding to overlapping sites (termed a, b, and c, in proximal to distal order) within the promoter region of the murine d7rp2e gene. in mus domesticus, strong binding occurs at site b, with weak binding at sites a and c; in m. pahari, strong binding is observed at sites a and c, with weak binding at site b. the m. pahari-specific occupancy pattern is associated with repression of transcription. presently, w ...19989608046
genetically determined resistance to flavivirus infection in wild mus musculus domesticus and other taxonomic groups in the genus mus.inherited resistance to flaviviruses in laboratory mice is a rare trait conferred by an autosomal dominant gene (flvr). to provide information on genetic resistance to flaviviruses in wild mice, we analysed (i) wild m. m. domesticus trapped in australia, and (ii) mice representing other species and subspecies in the genus mus. mice were screened for resistance relative to c3h/hej mice by intracerebral challenge with murray valley encephalitis virus or yellow fever virus, and breeding studies wer ...19989638142
evolution of transcriptional regulatory elements within the promoter of a mammalian gene.transcription of the murine d7rp2e gene is highly variable among species of the genus mus, indicating that extensive modifications in the gene's regulatory elements have occurred during evolution. since promoter regions are well known to harbor cis-acting information that controls gene transcription, we compared the sequence and function of the d7rp2e promoter in several mus species, with the goal of understanding the molecular mechanisms underlying the interspecies variations in expression. thr ...19989501498
temporal changes in the frequency of colicinogeny in escherichia coli from house mice.escherichia coli was isolated from feral house mice (mus domesticus) during the course of a mouse plague in the state of victoria, australia. the isolates were characterized for the production of colicins and their resistance to the co-occurring colicins. of the 447 isolates examined, 59% were found to be colicinogenic. phenotypic and pcr-based genotypic methods were used to determine the types of colicins being produced. colicin e2 was the most common, representing 27% of the colicin-producing ...19989720045
multiple copies of the ala-d gene are located at the lv locus in mus domesticus mice.incremental differences in delta-aminolevulinate dehydratase (ala-d; the second enzyme of the heme biosynthetic pathway) activity among inbred mouse strains can be attributed to variation in the number of copies of the ala-d gene. we have cloned and characterized the lv locus from an inbred mouse strain (dba/2j) that has three times the normal ala-d activity levels. the entire 12-kb ala-d gene plus 16 kb of flanking dna are found in 28-kb tandemly repeating units. we used the derived nucleotide ...19989521876
contact zones between chromosomal races of mus musculus domesticus. 1. temporal analysis of a hybrid zone between the cd chromosomal race (2n=22) and populations with the standard karyotype.a temporal analysis of a hybrid zone between chromosomal races of mus musculus domesticus was performed to evaluate its dynamics over a 20-year period. the transect chosen was one previously analysed by spirito et al. (1980) across the hycbrid zone between the chromosomal race cd 2n=22 (central italy) and the surrounding populations with standard karyotype (2n=40). the results show that 20 years of hybridization have not had any significant effect on the position of this hybrid zone, nor on the ...199910504430
y chromosome variation of mice and men.dna sequences from the nonrecombining portion of the y chromosome were compared with autosomal and x-linked sequences from mice and humans to test the neutral prediction that ratios of polymorphism to divergence are the same for different genes. intraspecific variation within mus domesticus was compared with divergence between m. domesticus and mus caroli for sry, a region 5' to sry, and four x-linked genes, hprt, plp, amg, and glra2. none of these comparisons revealed significantly reduced vari ...19989866208
wild isolates of murine cytomegalovirus induce myocarditis and antibodies that cross-react with virus and cardiac myosin.the laboratory-adapted k181 strain of murine cytomegalovirus (mcmv) induces both acute and chronic myocarditis, associated with autoantibodies to cardiac myosin, in susceptible balb/c mice. however, the k181 mcmv strain has been maintained in the laboratory for many years and may not resemble naturally occurring strains of mcmv in its ability to induce myocarditis. accordingly, six different isolates of mcmv from wild mus domesticus were compared with k181 mcmv for their ability to induce myocar ...19989741351
competitive behaviour in an island population of house mice, mus domesticus.because of the cryptic nature of small mammals inhabiting vegetation with widely scattered food resources, little is known about the behavioural mechanisms underlying their spatial dispersion. while house mice exploiting human-built environments attempt to exclude intruders aggressively from small territories that contain concentrated food resources, comparative tests show that the grassland mouse, m. spretus, establishes dominance relationships and then avoids sites used by dominant competitors ...19989819347
organisation of complex nuclear domains in somatic mouse cells.the number and associations of heterochromatin chromocenters, nucleoli, centromeres and telomeres were studied in the nucleus of different somatic cells of mus domesticus. fibroblasts of the cell line 3t3, kidney cells (primary culture), and bone marrow cells were used. the above mentioned nuclear and chromosome markers were identified by dapi/actinomycin d, indirect immunofluorescence with anti-centromere antibodies, silver impregnation for nucleolar proteins and fluorescence in situ hybridisat ...199910321023
mus and peromyscus chromosome homology established by fish with three mouse paint probes.fluorescence-labeled dna probes constructed from three whole house mouse (mus domesticus) chromosomes were hybridized to metaphase spreads from deer mouse (peromyscus maniculatus) to identify homologies between the species. mus chr 7 probe hybridized strongly to the ad-centromeric two-thirds of peromyscus chr 1q. most of mus 3 probe hybridized principally to two disjunct segments of peromyscus chr 3. mus chr 9 probe hybridized entirely to the whole peromyscus chr 7. three peromyscus linkage grou ...199910384049
crest staining of micronuclei from free-living rodents to detect environmental contamination in this work immunofluorescent antikinetochore (crest) staining was used to analyse bone marrow micronuclei (mn) from free-living animals belonging to four different rodent species. yellow-necked mice (apodemus flavicollis) and bank voles (clethrionomys glareolus) were trapped in the czech republic, algerian mice (mus spretus) in spain and house mice (mus musculus domesticus) in italy. animals were collected in areas displaying low or high environmental pollution in order to investigate the sens ...199910390506
recombination between subtypes creates a mosaic lineage of line-1 that is expressed and actively retrotransposing in the mouse genome.line-1, or l1, elements are retrotransposons that have amplified to high-copy number during the evolution of mammals. l1 appears to amplify in waves, spawning large numbers of progeny such that elements with distinct sequence features dominate the dispersal process in a given window of time. this process generates discrete subfamilies of l1 within mammalian genomes, with the oldest being remnants, or fossils, of earlier waves of amplification. in mice, at least three distinct subfamilies of l1 w ...19989677292
mhc-disassortative mating preferences reversed by mice (mus musculus domesticus) avoid mating with individuals that are genetically similar at the major histocompatibility complex (mhc). mice are able recognize mhc-similar individuals through specific odour cues. however, to mate disassortatively for mhc genes, individuals must have a referent, either themselves (self-inspection) or close kin (familial imprinting), with which to compare the mhc identity of potential mates. although studies on mhc-dependent mating preferences often assume ...19989718737
the origin of a robertsonian chromosomal translocation in house mice inferred from linked microsatellite markers.the western european house mouse, mus domesticus, includes many distinct robertsonian (rb) chromosomal races. two competing hypotheses may explain the distribution of rb translocations found in different populations: they may have arisen independently multiple times, or they may have arisen once and been spread through long-distance dispersal. we investigated the origin of the rb 5.15 translocation using six microsatellite loci linked to the centromeres of chromosomes 5 and 15 in 84 individuals ...199910605117
breast cancer incidence highest in the range of one species of house mouse, mus domesticus.incidence of human breast cancer (hbc) varies geographically, but to date no environmental factor has explained this variation. previously, we reported a 44% reduction in the incidence of breast cancer in women fully immunosuppressed following organ transplantation (stewart et al (1995) lancet 346: 796-798). in mice infected with the mouse mammary tumour virus (mmtv), immunosuppression also reduces the incidence of mammary tumours. dna with 95% identity to mmtv is detected in 40% of human breast ...200010646903
perception and recognition discriminated in the mouse auditory cortex by c-fos labeling.the functions of the fields of the mammalian auditory cortex in sound perception and recognition are unknown. we used fos (a protein of the inducible immediate-early gene c-fos) as a cellular marker of activated brain areas to show in the mouse (mus domesticus) that sound is processed differentially in auditory cortical fields according to its actual significance in a behavioral context. recognition, compared with perception of exactly the same sound, produced significantly less but well focused ...199910439460
isolation and microinjection of somatic cell-derived mitochondria and germline heteroplasmy in transmitochondrial present, there are no means for creation of relevant animal models of human mitochondrial dna (mtdna)-based diseases in a directed fashion. as an initial step towards this end, we have developed a microinjection technique for transfer of isolated, viable mitochondria between two mouse species. previously, we reported detection, by nested pcr with species-specific primer sets, of mus spretus mtdna in mus musculus domesticus blastocyts following zygote microinjection and culture. we now report ...199910481311
female sexual preferences differ in mus spicilegus and mus musculus domesticus: the role of familiarization and sexual experience.mating systems correspond to particular ecological conditions and result from proximate interactions between individuals. we compared the mating preferences of female mice of two species: the house mouse, mus musculus domesticus, and the mound-builder mouse, mus spicilegus. because of differences in their habitat, we expected to observe differences in their sexual preferences. we studied female preferences for a familiar or an unfamiliar male and the occurrence of copulation with the unfamiliar ...19989933543
panel of microsatellite markers for whole-genome scans and radiation hybrid mapping and a mouse family facilitate whole-genome scan experiments, we selected a panel of 128 microsatellite markers on the basis of spacing and polymorphism in the strains dba/2, balb/c, akr, c57bl/6, c57bl/10, a/j, c3h, 129/j, sjl/j, jf1, and pwb. many of the primer pairs were redesigned for better performance. the last four strains were not characterized previously using these markers. jf1 and pwb are particularly interesting for intersubspecific crosses offering high polymorphism. we provide allele size data for ...199910508847
experimental trypanosomiasis of natural hybrids between house mouse subspecies.this study characterises the extent of the susceptibility to parasites (first demonstrated with helminths) of hybrids between mus musculus domesticus and mus musculus musculus. experimental infections with trypanosoma musculi of m. m. domesticus, m. m. musculus and their natural hybrids have been performed to compare their level of resistance/susceptibility. it appears that contrary to the results with helminths, hybrid mice present the same level of resistance/susceptibility to the trypanosome ...199910501611
evolutionary rate of a gene affected by chromosomal position.genes evolve at different rates depending on the strength of selective pressure to maintain their function. chromosomal position can also have an influence [1] [2]. the pseudoautosomal region (par) of mammalian sex chromosomes is a small region of sequence identity that is the site of an obligatory pairing and recombination event between the x and y chromosomes during male meiosis [3] [4] [5] [6]. during female meiosis, x chromosomes can pair and recombine along their entire length. recombinatio ...199910508587
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