| [the differential carcinogenic effect of diethylnitrosamine in syrian and chinese hamsters]. | | 1966 | 5988952 |
| serum proteins of chinese hamsters and response of diabetics to tolbutamide and insulin. | | 1966 | 5936460 |
| studies in stress glycosuria. i. prolonged glycosuria in chinese hamsters after repeated stress. | | 2008 | 14104181 |
| [effects of sodium bromide and caffeine on the induction of pituitary adenomas by estrogen in chinese hamsters]. | | 1963 | 14089279 |
| observation of retinopathy in metahypophyseal diabetic chinese hamsters. | | 1963 | 14090728 |
| prediction of spontaneous hereditary diabetes mellitus in chinese hamsters by means of elevated alpha-2 serum levels. | | 1963 | 13950103 |
| pulpal and periodontal disease in a strain of chinese hamsters with hereditary diabetes mellitus. | | 1963 | 14022075 |
| adenocarcinoma of the pancreas in diabetes-prone chinese hamsters. | | 1961 | 14487364 |
| determination of cd50s and therapeutic indices of pentostam and ureastibamine in chinese hamsters. | | 2014 | 12988304 |
| experimental encephalitis virus infection in the chinese hamsters; a bacteriological and pathological study. | | 2014 | 14905510 |
| functional characterization of oligopeptide transporter 1 of dairy cows. | it is well known that peptides play a vital role in the nutrition and health of dairy cows. bovine oligopeptide transporter 1 (bpept1) is involved in the peptide transport process in the gastrointestinal tracts of dairy cows. however, little information is known in the characteristics of bpept1. therefore, the purpose of this study was to characterize bpept1 functionally using a mammalian cell expression system. the uptake of radiolabeled dipeptide glycyl-sarcosine ([3h]-gly-sar) into the bpept1 ... | 2018 | 29387385 |
| enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on chinese hamster ovary cells. | sialic acid plays important roles in stabilization and modulation of the interaction of molecules and membranes in organisms. due to its high electronegativity, sialic acid can promote binding effects of molecules and support the transportation of drugs and ions in cells. this also strengthens cells against degradation from glycosidases and proteases. hence sialic acid helps glycoproteins extend their half-lives and bioactivity. on the other hand, chinese hamster ovary (cho) cells have been wide ... | 2018 | 29439861 |
| preclinical characterization of three transient receptor potential vanilloid receptor 1 antagonists for early use in human intradermal microdose analgesic studies. | the transient receptor potential vanilloid receptor 1 (trpv1) is a nonselective cation channel involved in the mediation of peripheral pain to the central nervous system. as such, the trpv1 is an accessible molecular target that lends itself well to the understanding of nociceptive signalling. this study encompasses preclinical investigations of three molecules with the prospect to establish them as suitable analgesic model compounds in human intradermal pain relief studies. | 2018 | 29377430 |
| eel kisspeptins: identification, functional activity, and inhibition on both pituitary lh and gnrh receptor expression. | the european eel (anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. we previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of twokissgenes. in the present study, we cloned and sequencedkiss1andkiss2cdnas from the eel brain. the tissue distributions ofkiss1andkiss2transcripts, as investigated by quantitative re ... | 2017 | 29375473 |
| melanin nanoparticles: antioxidant activities and effects on γ-ray-induced dna damage in the mouse. | the radioprotective and antioxidant activities of melanin nanoparticles (mnp) were investigated in chinese hamster ovary (cho) cells in vitro and balb/c mice in vivo. the endpoints measured were cell viability, superoxide dismutase (sod) enzyme activity, malondialdehyde (mda) levels, dna damage (comet assay), and histopathological examination of tissues. irradiated groups showed decreased sod activity and increased mda levels. irradiation caused a 3-10-fold increase in comet parameters such as % ... | 2018 | 29555060 |
| assessment of the predictive capacity of the optimized in vitro comet assay using hepg2 cells. | evaluation of dna damage is critical during the development of new drugs because it is closely associated with genotoxicity and carcinogenicity. the in vivo comet assay to assess dna damage is globally harmonized as oecd tg 489. however, a comet test guideline that evaluates dna damage without sacrificing animals does not yet exist. the goal of this study was to select an appropriate cell line for optimization of the in vitro comet assay to assess dna damage. we then evaluated the predictivity o ... | 2018 | 29502738 |
| evaluating the interactions between red stingray (dasyatis akajei) melanocortin receptors and elephant shark (callorhinchus milii) mrap1 and mrap2 following stimulation with either stingray acth(1-24) or stingray des-acetyl-αmsh: a pharmacological study in chinese hamster ovary cells. | previous studies on bony vertebrate mc2r orthologs (i.e., ray finned fishes, amphibians, reptiles, birds, and mammals) have shown that these mc2r orthologs have an obligatory requirement for interaction with bony vertebrate mrap1 orthologs to a) allow for the trafficking of the mc2r ortholog to the plasma membrane; and b) to allow activation by acth, but not by any msh-sized ligand. in addition, previous studies have found that co-expression of teleost and mammalian mc4r orthologs with correspon ... | 2018 | 29524525 |
| comparative ecotoxicity of potential biofuels to water flea (daphnia magna), zebrafish (danio rerio) and chinese hamster (cricetulus griseus) v79 cells. | the ecotoxicity of two biofuel candidates (1‑octanol and 2‑butanone) was investigated by an integrative test strategy using three bioassays: the acute immobilisation test with water flea (d. magna), the fish embryo acute toxicity test with zebrafish (danio rerio) and the in vitro micronucleus assay with chinese hamster (cricetulus griseus) v79 cells. the median effective concentration (ec50) values were 14.9±0.66mgl-1for 1‑octanol, and 2152.1±44.6mgl-1for 2‑butanone in the d. magna test. both 1‑ ... | 2018 | 29524897 |
| role of microvesicles in the spread of herpes simplex virus type 1 in oligodendrocytic cells. | herpes simplex virus type 1 (hsv-1) is a neurotropic pathogen that can infect many types of cells and establishes latent infections in neurons of sensory ganglia. in some cases, the virus spreads into the cns causing encephalitis or meningitis. cells infected with several different types of viruses may secrete microvesicles containing viral proteins and rnas. in some instances, extracellular microvesicles harboring infectious virus have been found. here we describe the features of shedding micro ... | 2018 | 29514899 |
| analysis of peptide ligand specificity of different insect adipokinetic hormone receptors. | adipokinetic hormone (akh) is a highly researched insect neuropeptide that induces the mobilization of carbohydrates and lipids from the fat body at times of high physical activity, such as flight and locomotion. as a naturally occurring ligand, akh has undergone quite a number of amino acid changes throughout evolution, and in some insect species multiple akhs are present. akh acts by binding to a rhodopsin-like g protein-coupled receptor, which is related to the vertebrate gonadotropin-releasi ... | 2018 | 29439466 |
| development and characterization of anti-sheep podoplanin monoclonal antibodies pmab-253 and pmab-260. | anti-podoplanin (pdpn) monoclonal antibodies (mabs) are needed as markers for lymphatic endothelial cells or type i alveolar cells in immunohistochemical analyses. we have developed anti-pdpn mabs for many species, including humans, mice, rats, rabbits, dogs, cats, bovines, pigs, tasmanian devils, alpacas, tigers, whales, goats, horses, and bears. this study develops and characterizes anti-sheep pdpn (spdpn) mabs using cell-based immunization and screening (cbis) method. a rap14 tag was added to ... | 2020 | 32679010 |
| development of an anti-sheep podoplanin monoclonal antibody pmab-256 for immunohistochemical analysis of lymphatic endothelial cells. | sensitive and specific monoclonal antibodies (mabs) targeting podoplanin (pdpn) are needed for immunohistochemical analyses as a marker for lymphatic endothelial cells. we recently have developed anti-pdpn mabs against many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, tasmanian devil, alpaca, tiger, whale, goat, horse, and bear. however, anti-sheep pdpn (spdpn) has not yet been established. in this study, we used the cell-based immunization and screening method for the de ... | 2020 | 32349619 |
| development of an anti-bear podoplanin monoclonal antibody pmab-247 for immunohistochemical analysis. | sensitive and specific monoclonal antibodies (mabs) targeting podoplanin (pdpn) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because pdpn is known as a lymphatic endothelial cell maker in pathology. recently, we established anti-pdpn mabs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and tasmanian devil. however, anti-bear pdpn (bpdpn) has not been established yet. in this study, we immuni ... | 2019 | 31061899 |
| establishment of a monoclonal antibody pmab-233 for immunohistochemical analysis against tasmanian devil podoplanin. | monoclonal antibodies (mabs) against not only human, mouse, and rat but also rabbit, dog, cat, bovine, pig, and horse podoplanins (pdpns) have been established in our previous studies. pdpn is used as a lymphatic endothelial cell marker in pathological diagnoses. however, mabs against tasmanian devil pdpn (taspdpn), which are useful for immunohistochemical analysis, remain to be developed. herein, mice were immunized with taspdpn-overexpressing chinese hamster ovary (cho)-k1 (cho/taspdpn) cells, ... | 2019 | 30984883 |
| intact bioactivities and improved pharmacokinetic of the sl335-ifn-β-1a fusion protein that created by genetic fusion of sl335, a human anti-serum albumin fab, and human interferon-β. | recombinant human interferon beta (rifn-β) has long been used as a first-line treatment for multiple sclerosis (ms), and any attempt to develop a long-acting rifn-β is desirable since only one pegylated version of long-acting rifn-β-1a (plegridy) is currently available in clinics. previously, we reported that sl335, a human fab molecule specific to serum albumin, exhibits an extended serum half-life via utilizing the fcrn recycling mechanism. with the ultimate goal of developing a long-acting ri ... | 2019 | 30684504 |
| glyco-engineered long acting fgf21 variant with optimal pharmaceutical and pharmacokinetic properties to enable weekly to twice monthly subcutaneous dosing. | pharmacological administration of fgf21 analogues has shown robust body weight reduction and lipid profile improvement in both dysmetabolic animal models and metabolic disease patients. here we report the design, optimization, and characterization of a long acting glyco-variant of fgf21. using a combination of n-glycan engineering for enhanced protease resistance and improved solubility, fc fusion for further half-life extension, and a single point mutation for improving manufacturability in chi ... | 2018 | 29523796 |
| osmolality as a lever to modulate the n-glycolylneuraminicacid (neu5gc) level of a recombinant glycoprotein produced in chinese hamster ovary cells. | glycoproteins could be highly sialylated, and controlling the sialic acid levels for some therapeutic proteins is critical to ensure product consistency and efficacy. n-acetylneuraminic acid (neu5ac, or nana) and n-glycolylneuraminic acid (neu5gc, or ngna) are the two most common forms of sialic acids produced in mammalian cells. as neu5gc is not produced in humans and can elicit immune responses, minimizing neu5gc formation is important in controlling this quality attribute for complex glycopro ... | 2020 | 32542945 |
| high throughput generation of a resource of the human secretome in mammalian cells. | the proteins secreted by human tissues and blood cells, the secretome, are important both for the basic understanding of human biology and for identification of potential targets for future diagnosis and therapy. here, a high-throughput mammalian cell factory is presented that was established to create a resource of recombinant full-length proteins covering the majority of those annotated as 'secreted' in humans. the full-length dna sequences of each of the predicted secreted proteins were gener ... | 2020 | 32502629 |
| vanadium: risks and possible benefits in the light of a comprehensive overview of its pharmacotoxicological mechanisms and multi-applications with a summary of further research trends. | vanadium (v) is an element with a wide range of effects on the mammalian organism. the ability of this metal to form organometallic compounds has contributed to the increase in the number of studies on the multidirectional biological activity of its various organic complexes in view of their application in medicine. | 2020 | 32305626 |
| lack of acute toxicity and mutagenicity from recombinant epinephelus lanceolatus piscidin expressed in pichia pastoris. | the antimicrobial peptide (amp) piscidin was identified from epinephelus lanceolatus and demonstrated to possess antimicrobial and immune-related functions. supplementation of feed with recombinant epinephelus lanceolatus piscidin (rep)-expressing yeast pellets may minimize the excessive use of antibiotics and control pathogens in aquaculture or animal husbandry. however, before implementing rep as a supplement, it is necessary to understand whether it harbors any toxicity. since toxicological i ... | 2020 | 32290449 |
| gene (hprt) and chromosomal (mn) mutations of nickel metal powder in v79 chinese hamster cells. | nickel metal is a naturally occurring element used in many industrial and consumer applications. human epidemiological data and animal cancer bioassays indicate that nickel metal is not likely to be a human carcinogen. yet, nickel metal is classified as a suspected human carcinogen (clp) and possibly carcinogenic to humans (iarc). there are no reliable studies on the potential for nickel metal to induce gene and micronucleus (mn) mutations. to fill these datagaps and increase our understanding o ... | 2020 | 32014793 |
| hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody responses in rabbits but not in clinical trial participants. | a recombinant respiratory syncytial virus (rsv) fusion glycoprotein candidate vaccine (rsv-pref) manufactured in chinese hamster ovary cells was developed for immunization of pregnant women, to protect newborns against rsv disease through trans-placental antibody transfer. traces of a host-cell protein, hamster neogenin (haneo1), were identified in purified rsv-pref antigen material. given the high amino-acid sequence homology between haneo1 and human neogenin (huneo1), there was a risk that pot ... | 2020 | 31951765 |
| towards a new avenue for producing therapeutic proteins: microalgae as a tempting green biofactory. | most of the recent approved therapeutic proteins are multi-subunit biologics, which need glycosylation and disulfide bridges for their correct conformation and biological functions. currently, there exist many protein-based drugs that are mostly produced in the chinese hamster ovary (cho) cells. however, this expression system appears to associate with some limitations both in upstream and downstream processing steps, including low growth rate, sensitivity to different stresses and pathogens, an ... | 2020 | 31862234 |
| toxicity studies of lactobacillus plantarum ps128tm isolated from spontaneously fermented mustard greens. | : probiotics are extensively available to consumers; however, the use of probiotics may not always be safe, and there are few reports on their side effects, including those of lactobacillus. lactobacillus plantarum strain ps128tm isolated from spontaneously fermented mustard greens in taiwan was recently reported to exhibit probiotic properties. in this study, we aimed to assess the safety of strain ps128tm for use in humans via examining genotoxic and oral toxic effects using in vitro and in vi ... | 2019 | 31835837 |
| assessment of dna vaccine encoding toxoplasma gondii microneme complete gene and il-12 as adjuvant in balb/c mice. | toxoplasma gondii is an obligate intracellular protozoan parasite that causes toxoplasmosis in humans and animals. micronemes (mics) are effective candidates for dna vaccine. | 2019 | 31579446 |
| functional analysis of ldlr (low-density lipoprotein receptor) variants in patient lymphocytes to assess the effect of evinacumab in homozygous familial hypercholesterolemia patients with a spectrum of ldlr activity. | homozygous familial hypercholesterolemia is a rare disease usually caused by ldlr (low-density lipoprotein receptor) mutations. homozygous familial hypercholesterolemia is characterized by markedly elevated ldl-c (low-density lipoprotein cholesterol) levels and an extremely high risk of premature atherosclerotic cardiovascular disease. a phase 2, proof-of-concept study (nct02265952) demonstrated that evinacumab, a fully human monoclonal antibody to angptl3 (angiopoietin-like 3 protein), reduced ... | 2019 | 31578082 |
| the comet assay: assessment of in vitro and in vivo dna damage. | anthropogenic activities, indiscriminate and rapid industrialization as well as pursuance of a better life has led to an increase in the concentration of chemicals, like pesticides, automobile exhausts, and new chemical entities, in the environment, which have an adverse effect on all living organisms including humans. sensitive and robust test systems are thus required for accurate hazard identification and risk assessment. the comet assay has been used widely as a simple, rapid, and sensitive ... | 2019 | 31473963 |
| identification of lark as a novel and conserved g-quadruplex binding protein in invertebrates and vertebrates. | double-stranded dnas are usually present in the form of linear b-form double-helix with the base pairs of adenine (a) and thymine (t) or cytosine (c) and guanine (g), but g-rich dna can form four-stranded g-quadruplex (g4) structures, which plays important roles in transcription, replication, translation and protection of telomeres. in this study, a rna recognition motif (rrm)-containing protein, bmlark, was identified and demonstrated to bind g4 structures in the promoters of a transcription fa ... | 2019 | 31165881 |
| identification of cp77 as the third orthopoxvirus samd9 and samd9l inhibitor with unique specificity for a rodent samd9l. | orthopoxviruses (opxvs) have a broad host range in mammalian cells, but chinese hamster ovary (cho) cells are nonpermissive for vaccinia virus (vacv). here, we revealed a species-specific difference in host restriction factor samd9l as the cause for the restriction and identified orthopoxvirus cp77 as a unique inhibitor capable of antagonizing chinese hamster samd9l (chsamd9l). two known vacv inhibitors of samd9 and samd9l (samd9&l), k1 and c7, can bind human and mouse samd9&l, but neither can b ... | 2019 | 30918078 |
| new insight into the classification and evolution of glucose transporters in the metazoa. | because glucose is an essential energy source for living organisms, glucose transporters (gluts) are present in all species worldwide. encoded by the solute carrier family 2 gene family, the glut proteins generally have 12 transmembrane helices (tmhs). in total, 14 glut proteins have been identified in humans (hgluts), and they are divided into 3 classes on the basis of their transport characteristics and sequence similarities. herein, we report the use of protein sequence similarity networks (s ... | 2019 | 30888203 |
| simultaneous expression of clophensor and slc26a3 reveals the nature of endogenous oxalate transport in cho cells. | clophensor, a fluorescent fusion protein, is a dual function biosensor that has been utilized as a tool for the simultaneous measurement of intracellular chloride and ph in cells. clophensor has traditionally been used in conjunction with fluorescence microscopy for single cell measurements. here, we present a promising multi-well format advancement for the use of clophensor as a potential high-throughput method capable of measuring fluorescence signal intensity across a well of confluent cells ... | 2019 | 30837228 |
| identification of verapamil binding sites within human kv1.5 channel using mutagenesis and docking simulation. | the phenylalkylamine class of l-type ca2+ channel antagonist verapamil prolongs the effective refractory period (erp) of human atrium, which appears to contribute to the efficacy of verapamil in preventing reentrant-based atrial arrhythmias including atrial fibrillation. this study was designed to investigate the molecular and electrophysiological mechanism underlying the action of verapamil on human kv1.5 (hkv1.5) channel that determines action potential duration and erp in human atrium. | 2019 | 30816676 |
| incorporation of cd40 ligand or granulocyte-macrophage colony stimulating factor into hantaan virus (htnv) virus-like particles significantly enhances the long-term immunity potency against htnv infection. | hantavirus infections cause severe haemorrhagic fever with renal syndrome (hfrs) in humans and are associated with high fatality rates. in 2017, numerous outbreaks were reported in china and germany. this represents a significant public-healthcare issue with no effective hfrs vaccines that offer a long-term immune response. in this study, we investigated the long-term humoral and cellular immune responses and protective immunity of hantaan virus (htnv) granulocyte-macrophage colony stimulating f ... | 2019 | 30657443 |
| kcnq1 rescues tmc1 plasma membrane expression but not mechanosensitive channel activity. | transmembrane channel-like protein isoform 1 (tmc1) is essential for the generation of mechano-electrical transducer currents in hair cells of the inner ear. tmc1 disruption causes hair cell degeneration and deafness in mice and humans. although thought to be expressed at the cell surface in vivo, tmc1 remains in the endoplasmic reticulum when heterologously expressed in standard cell lines, precluding determination of its roles in mechanosensing and pore formation. here, we report that the kcnq ... | 2019 | 30613966 |
| glyco-engineered cho cell lines producing alpha-1-antitrypsin and c1 esterase inhibitor with fully humanized n-glycosylation profiles. | recombinant chinese hamster ovary (cho) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have n-glycosylation profiles similar, but not identical, to humans. due to differences in n-glycan moieties, two members of the serpin superfamily, alpha-1-antitrypsin (a1at) and plasma protease c1 inhibitor (c1inh), are currently derived from human plasma for treating a1at and c1inh deficiency. deriving therapeutic proteins from human plasma is generally a cost-in ... | 2019 | 30513349 |
| exposure to the fungicide captan induces dna base alterations and replicative stress in mammalian cells. | the classification of the fungicide captan (cas number: 133-06-2) as a carcinogen agent is presently under discussion. despite the mutagenic effect detected by the ames test and carcinogenic properties observed in mice, the genotoxicity of this pesticide in humans is still unclear. new information is needed about its mechanism of action in mammalian cells. here, we show that chinese hamster ovary (cho) cells exposed to captan accumulate fpg-sensitive dna base alterations. in cho and hela cells, ... | 2019 | 30471166 |
| na+ /ca2+ exchanger 1 is a key mechanosensitive molecule of the esophageal myenteric neurons. | our earlier studies showed that mechanical stretch activates inhibitory motor neurons of the oesophagus; however, the underlying molecular mechanisms are unclear. here, we sought to examine if na+ /ca2+ exchanger 1 (ncx1) is responsible for the mechanosensitivity in the esophageal myenteric neurons (emn) of rats and humans. | 2019 | 30466198 |
| early porcine sapovirus infection disrupts tight junctions and uses occludin as a coreceptor. | the genus sapovirus belongs to the family caliciviridae, and its members are common causative agents of severe acute gastroenteritis in both humans and animals. some caliciviruses are known to use either terminal sialic acids or histo-blood group antigens as attachment factors and/or cell surface proteins, such as cd300lf, cd300ld, and junctional adhesion molecule 1 of tight junctions (tjs), as receptors. however, the roles of tjs and their proteins in sapovirus entry have not been examined. in ... | 2019 | 30463963 |
| phosphorylation of extracellular signal-regulated kinase as a biomarker for cannabinoid receptor 2 activation. | cannabinoid receptor 2 (cb2r) is a therapeutic target in inflammatory diseases; its activation by agonists provides important clinical information, but there are currently no methods to quantify cb2r activation in humans. chinese hamster ovary (cho)-k1 cells and mouse and human whole blood cells were used for experiments. cb2r was activated in cells by treatment with the agonist cp55,940. cells were also pretreated with proprietary compound a and b (experimental agonists). we developed our metho ... | 2018 | 30450437 |
| binding characteristics of two oxytocin variants and vasopressin at oxytocin receptors from four primate species with different social behavior patterns. | a clade of new world monkeys (nwms) exhibits considerable diversity in both oxytocin (ot) ligand and oxytocin receptor (otr) structure. most notable is the variant pro8-ot, with proline instead of leucine at the eighth position, resulting in a rigid bend in the peptide backbone. a higher proportion of species that express pro8-ot also engage in biparental care and social monogamy. when marmosets (genus callithrix), a biparental and monogamous pro8-ot nwm species, are administered the ancestral l ... | 2018 | 30068728 |
| defective mucin-type glycosylation on α-dystroglycan in cog-deficient cells increases its susceptibility to bacterial proteases. | deficiency in subunits of the conserved oligomeric golgi (cog) complex results in pleiotropic defects in glycosylation and causes congenital disorders in humans. insight regarding the functional consequences of this defective glycosylation and the identity of specific glycoproteins affected is lacking. a chemical glycobiology strategy was adopted to identify the surface glycoproteins most sensitive to altered glycosylation in cog-deficient chinese hamster ovary (cho) cells. following metabolic l ... | 2018 | 30049793 |
| exposure to solar light reduces cytotoxicity of sewage effluents to mammalian cells: roles of reactive oxygen and nitrogen species. | sewage effluents can contain hundreds of toxic pollutants, making them a risk to humans when involved in drinking water. it is therefore important to evaluate the cytotoxicity of sewage effluents to mammalian cells. solar light might influence the water quality of sewage effluents after their discharge into lakes or rivers, altering their cytotoxicity. in this study, natural solar light was found to lower the cytotoxicity of sewage effluents to chinese hamster ovary (cho) cells. cytotoxicity of ... | 2018 | 30015097 |
| lion/web: a web-based ontology enrichment tool for lipidomic data analysis. | a major challenge for lipidomic analyses is the handling of the large amounts of data and the translation of results to interpret the involvement of lipids in biological systems. | 2019 | 31141612 |
| establishment of a monoclonal antibody pmab-231 for tiger podoplanin. | podoplanin (pdpn), also known as t1alpha, has been used as a lung type i alveolar cell marker in the pathophysiological condition. although we have established several monoclonal antibodies (mabs) against mammalian pdpns, mabs against tiger pdpn (tigpdpn), which are useful for immunohistochemical analysis, remain to be developed. in this study, we immunized mice with tigpdpn-overexpressing chinese hamster ovary (cho)-k1 cells (cho/tigpdpn) and screened hybridomas producing mabs against tigpdpn u ... | 2019 | 31009336 |
| detection of tiger podoplanin using the anti-cat podoplanin monoclonal antibody pmab-52. | podoplanin (pdpn) is expressed in type i alveolar cells of lung but not in type ii alveolar cells. pdpn is also known as a specific lymphatic endothelial cell marker because pdpn is not expressed in vascular endothelial cells. pdpns of several animals have been characterized using specific anti-pdpn monoclonal antibodies (mabs): pmab-1, pmab-2, pmab-32, pmab-38, pmab-44, and pmab-52 for mouse, rat, rabbit, dog, bovine, and cat pdpns, respectively. in this study, we investigated the possible cros ... | 2018 | 30362928 |
| the acute phase protein orosomucoid 1 is upregulated in early lactation but does not trigger appetite-suppressing stat3 signaling via the leptin receptor. | dairy cows consume inadequate amounts of feed in early lactation and during conditions and diseases such as excessive fatness, heat stress, and infectious diseases. affected cows often experience increases in plasma concentrations of acute phase proteins consistent with the negative effect of inflammation on appetite. the acute phase protein orosomucoid 1 (orm1), also known as alpha-1-acid glycoprotein, was recently reported to reduce appetite in the mouse through its ability to bind the full-le ... | 2020 | 32229118 |
| single-cell transcriptome analyses reveal heterogeneity in suspension cultures and clonal markers of cho-k1 cells. | cell-to-cell variability in cell populations arises from a combination of intrinsic factors and extrinsic factors related to the milieu. however, the heterogeneity of high cell density suspension cultures for therapeutic protein production remains unknown. here, we illustrate the increasing heterogeneity in the cellular transcriptome of serum-free adapted cho k1 cells during high cell density suspension culture over time without concomitant changes in the genomic sequence. cell cycle-dependent s ... | 2020 | 33179258 |
| surface antifouling modification on polyethylene filtration membranes by plasma polymerization. | surface modification on microporous polyethylene (pe) membranes was facilitated by plasma polymerizing with two hydrophilic precursors: ethylene oxide vinyl ether (eo1v) and diethylene oxide vinyl ether (eo2v) to effectively improve the fouling against mammalian cells (chinese hamster ovary, cho cells) and proteins (bovine serum albumin, bsa). the plasma polymerization procedure incorporated uniform and pin-hole free ethylene oxide-containing moieties on the filtration membrane in a dry single-s ... | 2020 | 33172217 |
| metabolic profiling of cho cell cultures at different working volumes and agitation speeds using spin tube reactors. | culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on chinese hamster ovary (cho) cells. despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant cho cell lines. here we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production and cell metabolism of two clonally-derived cho ... | 2020 | 33169492 |
| control of igg glycosylation in cho cell perfusion cultures by greba mathematical model supported by a novel targeted feed, tafe. | the n-linked glycosylation pattern is an important quality attribute of therapeutic glycoproteins. it has been reported by our group and by others that different carbon sources, such as glucose, mannose and galactose, can differently impact the glycosylation profile of glycoproteins in mammalian cell culture. acting on the sugar feeding is thus an attractive strategy to tune the glycan pattern. however, in case of feeding of more than one carbon source simultaneously, the cells give priority to ... | 2020 | 33161144 |
| the cag promoter maintains high-level transgene expression in hek293 cells. | the vast majority of therapeutic recombinant proteins are produced in mammalian cell lines. however, proteins generated in non-human cell lines, such as chinese hamster ovary (cho) cells, are decorated with human-like glycan structures that differ from those of human cells, and these may induce immunogenic responses in human cells. human embryonic kidney cells (hek293f) are also extensively used as hosts for the expression of recombinant therapeutic proteins, but their utility is limited by the ... | 2020 | 33155423 |
| a novel lamprey antibody sequence to multimerize and increase the immunogenicity of recombinant viral and bacterial vaccine antigens. | hemagglutinin, the major surface protein of influenza viruses, was recombinantly expressed in eukaryotic cells as a monomer instead of its native trimer, and was only immunogenic when administered with an adjuvant [pion et al. 2014]. in order to multimerize this antigen to increase its immunogenicity, a cysteine-rich peptide sequence found at the extreme c-terminus of lamprey variable lymphocyte receptor (vlr)-b antibodies was fused to various recombinant hemagglutinin (rha) proteins from a and ... | 2020 | 33153770 |
| igist-a kinetic bioassay for pertussis toxin based on its effect on inhibitory gpcr signaling. | detection of pertussis toxin (ptx) activity is instrumental for the development and manufacturing of pertussis vaccines. these quality and safety measures require thousands of mice annually. here, we describe interference in gαi-mediated signal transduction (igist), an animal-free kinetic bioassay for detection of ptx, by measuring its effect on inhibitory g protein-coupled receptor (gpcr) signaling. ptx adp-ribosylates inhibitory α-subunits of the heterotrimeric g proteins, thereby perturbing t ... | 2020 | 33147407 |
| adiponectin forms a complex with atherogenic ldl and inhibits its downstream effects. | adiponectin, an adipocyte-derived protein, has anti-atherogenic and anti-diabetic effects, but how it confers the anti-atherogenic effects is not well understood. to study the anti-atherogenic mechanisms of adiponectin, we examined whether it interacts with atherogenic low-density lipoprotein (ldl) to attenuate ldl's atherogenicity. l5, the most electronegative subfraction of ldl, induces atherogenic responses similarly to copper-oxidized ldl (oxldl). unlike native ldl endocytosed via the ldl re ... | 2020 | 33144330 |
| bioluminescence as a sensitive electroporation indicator in sub-microsecond and microsecond range of electrical pulses. | the cell membrane permeabilization in electroporation studies is usually quantified using fluorescent markers such as propidium iodide (pi) or yo-pro, while chinese hamster ovary cell line frequently serves as a model. in this work, as an alternative, we propose a sensitive methodology for detection and analysis of electroporation phenomenon based on bioluminescence. luminescent mice myeloma sp2/0 cells (transfected using luciferase-pcdna3 plasmid) were used as a cell model. electroporation has ... | 2020 | 33142215 |
| trans-cinnamaldehyde prevents oxidative stress-induced apoptosis in v79-4 chinese hamster lung fibroblasts through the nrf2-mediated ho-1 activation. | oxidative stress, which is characterized by overproduction of reactive oxygen species (ros), is considered a major risk factor associated with fibroblast death in severe lung diseases such as idiopathic pulmonary fibrosis. trans-cinnamaldehyde (tca), the major phytochemical constituent in cinnamon, is known to possess strong anti-oxidant activity. however, whether tca can defend lung fibroblasts against oxidative injury remains to be elucidated. therefore, this study was conducted to investigate ... | 2020 | 33132316 |
| formation of carbonaceous and nitrogenous iodinated disinfection byproducts from biofilm extracellular polymeric substances by the oxidation of iodide-containing waters with lead dioxide. | lead dioxide (pbo2) is an important form of lead mineral scales in drinking water pipes. iodide (i-) widely presents in source waters and can be thermodynamically oxidized by pbo2 to the reactive iodine species (i2/hoi). biofilm extracellular polymeric substances (eps) are nonnegligible precursors of disinfection byproducts (dbps). the aim was to study the oxidation of i- by pbo2 and formation of iodinated dbps (i-dbps) from eps. at a high molar ratio of pbo2 to i- (> 100), the observed rate con ... | 2020 | 33128980 |
| production of trimeric sars-cov-2 spike protein by cho cells for serological covid-19 testing. | we describe scalable and cost-efficient production of full length, his-tagged severe acute respiratory syndrome coronavirus 2 (sars-cov-2) spike glycoprotein trimer by chinese hamster ovary (cho) cells that can be used to detect sars-cov-2 antibodies in patient sera at high specificity and sensitivity. transient production of spike in both human embryonic kidney (hek) and cho cells mediated by polyethyleneimine was increased significantly (up to 10.9-fold) by a reduction in culture temperature t ... | 2020 | 33128388 |
| methylthioadenosine (mta) boosts cell-specific productivities of chinese hamster ovary cultures: dosage effects on proliferation, cell cycle and gene expression. | a major goal for process and cell engineering in the biopharmaceutical industry is enhancing production through increasing volumetric and cell-specific productivities (csp). here, we present 5'-deoxy-5'-(methylthio)adenosine (mta), the degradation product of s-(5'-adenosyl)-l-methionine (sam), as a highly attractive native additive which can boost csp by 79% when added to exponentially growing cells at a concentration of 250-300 μm. notably, cell viability and cell size remain higher than in non ... | 2020 | 33128321 |
| evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombinant antibodies produced in chinese hamster ovary (cho) cell lines. | with the advent of highly sensitive technologies such as tandem mass spectrometry and next-generation sequencing, recombinant antibodies are now routinely analyzed for the presence of low-level sequence variants including amino acid misincorporations. during mab cell culture process development, we found that proline was replaced with the non-canonical amino acid, hydroxyproline, in the protein sequence. we investigated the relationship between proline content in the cell culture media and proli ... | 2020 | 33119652 |
| ptselect™: a post-transcriptional technology that enables rapid establishment of stable cho cell lines and surveillance of clonal variation. | currently, stable chinese hamster ovary cell lines producing therapeutic, recombinant proteins are established either by antibiotic and/or metabolic selection. here, we report a novel technology, ptselect™ that utilizes an sirna cloned upstream of the gene of interest (goi) that is processed to produce functional ptselect™-sirnas, which enable cell enrichment. cells with stably integrated goi are selected and separated from cells without goi by transfecting cd4/sirna mrna regulated by ptselect™- ... | 2020 | 33115662 |
| impact of polyallylamine hydrochloride on gene expression and karyotypic stability of multidrug resistant transformed cells. | the synthetic polymer, polyallylamine hydrochloride (paa), is found in a variety of applications in biotechnology and medicine. it is used in gene and sirna transfer, to form microcapsules for targeted drug delivery to damaged and tumor cells. conventional chemotherapy often does not kill all cancer cells and leads to multidrug resistance (mdr). until recently, studies of the effects of paa on cells have mainly focused on their morphological and genetic characteristics immediately or several hou ... | 2020 | 33096691 |
| high-resolution three-dimensional chromatin profiling of the chinese hamster ovary cell genome. | chinese hamster ovary (cho) cell lines are the pillars of a multi-billion dollar biopharmaceutical industry producing recombinant therapeutic proteins. the effects of local chromatin organisation and epigenetic repression within these cell lines result in unpredictable and unstable transgene expression following random integration. limited knowledge of the cho genome and its higher-order chromatin organisation has thus far impeded functional genomics approaches required to tackle these issues. h ... | 2020 | 33095445 |
| improving antibody production in stably transfected cho cells by crispr-cas9-mediated inactivation of genes identified in a large-scale screen with chinese hamster-specific sirnas. | the chinese hamster ovary (cho) cell line is commonly used for the production of biotherapeutics. as cell productivity directly affects the cost of production, methods are developed to manipulate the expression of specific genes that are known to be involved in protein synthesis, folding, and secretion to increase productivity. however, there are no large-scale cho-specific functional screens to identify novel gene targets that impact the production of secreted recombinant proteins. here, a larg ... | 2020 | 33079482 |
| establishment of fast-growing serum-free immortalised cells from chinese hamster lung tissues for biopharmaceutical production. | chinese hamster (cricetulus griseus) ovary-derived chinese hamster ovary (cho) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins. they are also valuable for their ability to grow in serum-free suspension cultures. in this study, we established a cell line derived from lung tissue of chinese hamsters, named chinese ha ... | 2020 | 33077772 |
| development of a high yielding bioprocess for a pre-fusion rsv subunit vaccine. | respiratory syncytial virus (rsv) is a highly contagious virus causing severe infection in infants and the elderly. various approaches are being used to develop an effective rsv vaccine. the rsv fusion (f) subunit, particularly the cleaved trimeric pre-fusion f, is one of the most promising vaccine candidates under development. the pre-fusion conformation elicits the majority of neutralizing antibodies during natural infection. however, this pre-fusion conformation is metastable and prone to con ... | 2020 | 33068697 |
| biological activities of a new crotamine-like peptide from crotalus oreganus helleri on c2c12 and cho cell lines, and ultrastructural changes on motor endplate and striated muscle. | crotamine and crotamine-like peptides are non-enzymatic polypeptides, belonging to the family of myotoxins, which are found in high concentration in the venom of the crotalus genus. helleramine was isolated and purified from the venom of the southern pacific rattlesnake, crotalus oreganus helleri. this peptide had a similar, but unique, identity to crotamine and crotamine-like proteins isolated from other rattlesnakes species. the variability of crotamine-like protein amino acid sequences may al ... | 2020 | 33065200 |
| raman spectra-based deep learning: a tool to identify microbial contamination. | deep learning has the potential to enhance the output of in-line, on-line, and at-line instrumentation used for process analytical technology in the pharmaceutical industry. here, we used raman spectroscopy-based deep learning strategies to develop a tool for detecting microbial contamination. we built a raman dataset for microorganisms that are common contaminants in the pharmaceutical industry for chinese hamster ovary (cho) cells, which are often used in the production of biologics. using a c ... | 2020 | 33063423 |
| the quantitative analysis of the mechanism involved in pertussis toxin-mediated cell clustering and its implications in the in vitro quality control of diphtheria tetanus and whole cell pertussis vaccines. | some of the adverse side-effects such as leukocytosis, hyperinsulinemia, hypoglycemia and sensitization to histamine, caused by diphtheria, tetanus and whole cell pertussis (dtwp) vaccines are related to the presence of non-inactivated pertussis toxin residues (niptxr). the chinese hamster ovary (cho) cell clustering assay is an in vitro assay to measure niptxr in dtwp vaccines based on the ability of active pertussis toxin (ptx) to cause cellular clustering. to study the biochemical mechanism i ... | 2020 | 33059000 |
| polymer fraction including exosomes derived from chinese hamster ovary cells promoted their growth during serum-free repeated batch culture. | while continuous (perfusion) culture of mammalian cells might reduce the reactor size owing to the high cell density, there is the problem of higher medium cost; however, this problem is expected to be solved by the reuse of growth-promoting components in the culture supernatant. the polymer fraction (pf, 10 kda-220 nm) collected from the supernatant of serum-free repeated-batch culture of chinese hamster ovary (cho) cells in not only adhesion but also suspension promoted the cell growth in resp ... | 2020 | 33051156 |
| hypericin, a medicinal compound from st. john's wort, inhibits genotoxicity induced by mutagenic agents in v79 cells. | this study evaluated the cytotoxic, genotoxic, and the modulatory effects on dna damage of hypericin in chinese hamster lung fibroblasts (v79 cells). the hypericin is a natural polycyclic quinone, mainly extracted from st. john's wort (hypericum perforatum l.). along with hyperforin, the hypericins are responsible for the antidepressant activity of st. john's wort. cytotoxicity was assessed by the xtt colorimetric assay and the nuclear division index (ndi). the genotoxic activity was studied by ... | 2020 | 33050761 |
| systematic identification of safe harbor regions in the cho genome through a comprehensive epigenome analysis. | the chinese hamster ovary (cho) cell lines that are used to produce commercial quantities of therapeutic proteins commonly exhibit a decrease in productivity over time in culture, a phenomenon termed production instability. random integration of the transgenes encoding the protein of interest into locations in the cho genome that are vulnerable to genetic and epigenetic instability often causes production instability through copy number loss and silencing of expression. several recent publicatio ... | 2020 | 33049068 |
| distinct functions of acyl/alkyl dihydroxyacetonephosphate reductase in peroxisomes and endoplasmic reticulum. | plasmalogens are a subclass of ether glycerophospholipids characterized by a vinyl-ether bond at the sn-1 position of the glycerol backbone. plasmalogen biosynthesis is initiated in peroxisomes. at the third step of plasmalogen synthesis, alkyl-dihydroxyacetonephosphate (dhap) is enzymatically reduced to 1-alkyl-sn-glycero-3-phospate by acyl/alkyl dhap reductase (adhapr), whose activity is found in both peroxisomes and microsomes. we herein show that knockdown of adhapr in hela cells reduced the ... | 2020 | 33042986 |
| chlorella vulgaris extract as a serum replacement that enhances mammalian cell growth and protein expression. | the global cell culture market is experiencing significant growth due to the rapid advancement in antibody-based and cell-based therapies. both rely on the capacity of different living factories, namely prokaryotic and eukaryotic cells, plants or animals for reliable and mass production. the ability to improve production yield is of important concern. among many strategies pursued, optimizing the complex nutritional requirements for cell growth and protein production has been frequently performe ... | 2020 | 33042965 |
| systematically gap-filling the genome-scale metabolic model of cho cells. | chinese hamster ovary (cho) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. in this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. these models need to be regularly updated in order to include the latest biochemical data of the cells, and to increase their predictive power. here, we provide an update to icho1766, the metabolic model of cho cells. | 2020 | 33040240 |
| establishment of microbeam radiation therapy at a small-animal irradiator. | microbeam radiation therapy is a preclinical concept in radiation oncology. it spares normal tissue more effectively than conventional radiation therapy at equal tumor control. the radiation field consists of peak regions with doses of several hundred gray, whereas doses between the peaks (valleys) are below the tissue tolerance level. widths and distances of the beams are in the submillimeter range for microbeam radiation therapy. a similar alternative concept with beam widths and distances in ... | 2020 | 33038461 |
| 1-methylpyrene induces chromosome loss and mitotic apparatus damage in a chinese hamster v79-derived cell line expressing both human cyp1a2 and sulfotransferase 1a1. | 1-methylpyrene (1-mp) is a ubiquitous environmental pollutant and rodent carcinogen. its mutagenic activity depends on sequential activation by various cyp and sulfotransferase (sult) enzymes. previously we have observed induction of micronuclei and mitotic arrest by 1-mp in a chinese hamster (v79)-derived cell line expressing both human cyp1a2 and sult1a1 (v79-hcyp1a2-hsult1a1), however, the mode of chromosome damage and the involvement of mitotic tubulin structures have not been clarified. in ... | 2020 | 33035519 |
| inhibition of poly(adp-ribose) polymerase induces synthetic lethality in brip1 deficient ovarian epithelial cells. | pathogenic variations in the homologous recombination (hr) gene, brca1 interacting protein c-terminal helicase 1 (brip1) increase the risk for ovarian cancer. parp inhibitors (parpi) exert a synthetic lethal effect in brca-mutated ovarian cancers. effective hr requires cooperation between brca1 and brip1; therefore, brip1-incompetancy may predict vulnerability to synthetic lethality. here we investigated the response of ovarian epithelial cells with defective brip1 function to parpi, and compare ... | 2020 | 33032822 |
| development of a new microdosimetric biological weighting function for the rbe10assessment in case of the v79 cell line exposed to ions from1h to238u. | an improved biological weighting function (ibwf) is proposed to phenomenologically relate microdosimetric lineal energy probability density distributions with the relative biological effectiveness (rbe) for the in vitro clonogenic cell survival (surviving fraction = 10%) of the most commonly used mammalian cell line, i.e. the chinese hamster lung fibroblasts (v79). the ibwf, intended as a simple and robust tool for a fast rbe assessment to compare different exposure conditions in particle therap ... | 2020 | 33032273 |
| a novel method to detect autoantibodies against platelets in patients with immune thrombocytopenia. | to examine anti-platelet autoantibodies in patients with immune thrombocytopenia (itp) not only provides solid evidence for diagnosis, and also helps to select an individualized strategy for the treatment. the aim of this study is to develop a novel cell-based assay to detect autoantibodies in itp patients. | 2020 | 33031805 |
| transient multivalent nanobody targeting to cd206-expressing cells via ph-degradable nanogels. | to target nanomedicines to specific cells, especially of the immune system, nanobodies can be considered as an attractive tool, as they lack the fc part as compared to traditional antibodies and, thus, prevent unfavorable fc-receptor mediated mistargeting. for that purpose, we have site-specifically conjugated cd206/mmr-targeting nanobodies to three types of dye-labeled nanogel derivatives: non-degradable nanogels, acid-degradable nanogels (with ketal crosslinks), and single polymer chains (also ... | 2020 | 33019594 |
| mutagenicity of 7-ketocholesterol in cho cells: the role of lipid peroxidation. | as an important cholesterol oxide, 7-ketocholesterol plays a deleterious role in the occurrence of cancer. although the fact had been proved that 7-ketocholesterol could induce several biological phenomena, including apoptosis, dna damage, et al., this issue whether 7-ketocholesterol led to mutagenesis in mammalian cells remains largely unexplored. here, we investigated the major role of lipid peroxidation in the genotoxic response to 7-ketocholesterol in chinese hamster ovary (cho) cells. the r ... | 2020 | 33017620 |
| a novel kv7.3 variant in the voltage-sensing s4 segment in a family with benign neonatal epilepsy: functional characterization and in vitro rescue by β-hydroxybutyrate. | pathogenic variants in kcnq2 and kcnq3, paralogous genes encoding kv7.2 and kv7.3 voltage-gated k+ channel subunits, are responsible for early-onset developmental/epileptic disorders characterized by heterogeneous clinical phenotypes ranging from benign familial neonatal epilepsy (bfne) to early-onset developmental and epileptic encephalopathy (dee). kcnq2 variants account for the majority of pedigrees with bfne and kcnq3 variants are responsible for a much smaller subgroup, but the reasons for ... | 2020 | 33013448 |
| a rare hcn4 variant with combined sinus bradycardia, left atrial dilatation, and hypertrabeculation/left ventricular noncompaction phenotype. | hcn4 variants have been reported to cause combined sick sinus syndrome (sss) and left ventricular noncompaction (lvnc) cardiomyopathy. this relationship has been proven in few cases and no previous patients have associated left atrial dilatation (lad). our objective was to study a familial disorder characterized by sss, lad, and hypertrabeculation/lvnc and to identify the underlying genetic and electrophysiological characteristics. | 2020 | 33008772 |
| improving the expression of anti-il-2rα monoclonal antibody in the cho cells through optimization of the expression vector and translation efficiency. | the growing need for monoclonal antibodies (mabs) necessitates the development of novel and efficient production approaches. regulatory elements like ubiquitous chromatin-opening elements (ucoes) have been employed for improvement of the mab expression in the chinese hamster ovary (cho) cells. sineups are a class of long non-coding rnas, which can improve the translation of partly overlapping mrnas. a combination of these two elements might lead to higher production of mabs. therefore, the curre ... | 2020 | 33007349 |
| animal model and bioinformatics analyses suggest the timp1/mmp9 axis as a potential biomarker in oral squamous cell carcinoma. | oral squamous cell carcinoma (oscc) is a common malignant tumor of the head and neck. however, the molecular mechanism underlying its development and progression is yet unclear. genes that are differentially expressed, that is, differentially expressed genes (degs), between normal and diseased tissues are believed to be involved in disease development and progression. to identify the degs in oscc and explore their role in occurrence and progression, we established a chinese hamster oscc model, d ... | 2020 | 33006223 |
| toxicity assessment of gryllus bimaculatus (a type of cricket) glycosaminoglycan. | we performed general toxicity studies of gryllus bimaculatus (two-spotted cricket) glycosaminoglycan (gbg), including a single, 4-week repeated oral dose toxicity test in icr mice, and short-term genotoxicity tests. the mutagenic potential of the purified gbg was non-genotoxic when it was evaluated using short-term genotoxicity tests, namely ames, chromosome aberration (ca), and micronuclei (mn) tests. in salmonella typhimurium and escherichia coli assays, gbg did not produce any mutagenic respo ... | 2020 | 33005591 |
| pharmacokinetic prediction of an antibody in mice based on an in vitro cell-based approach using target receptor-expressing cells. | in vivo pharmacokinetics (pk) studies using mice and monkeys are the main approaches for evaluating and predicting the pk of antibodies, and there is a strong demand for methods that do not require animal experiments. in this work, we focused on quantitatively predicting the nonlinear pk of an antibody based on cell-based assays. an anti-mouse fc gamma receptor iib antibody was used as a model antibody. to determine the pk parameters related to nonspecific elimination in vivo, the plasma concent ... | 2020 | 33004886 |
| biological activities of a recombinant fortilin from fenneropenaeus merguiensis. | human fortilin, an antiapoptotic protein, has also been implicated in several diseases; however, several potential uses of fortilin have also been proposed. bearing the implications of fortilin in mind, fortilin analog, which has no complication with diseases, is required. since a recombinant full-length fortilin from fenneropenaeus merguiensis (rfm-fortilin (fl)) reported only 44% (3e-27) homologous to human fortilin, therefore the biological activities of the fm-fortilin (fl) and its fragments ... | 2020 | 33002062 |
| a defucosylated anti‑cd44 monoclonal antibody 5‑mg2a‑f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma. | cd44 is widely expressed on the surface of most tissues and all hematopoietic cells, and regulates many genes associated with cell adhesion, migration, proliferation, differentiation, and survival. cd44 has also been studied as a therapeutic target in several cancers. previously, an anti‑cd44 monoclonal antibody (mab), c44mab‑5 (igg1, kappa) was established by immunizing mice with cd44‑overexpressing chinese hamster ovary (cho)-k1 cells. c44mab‑5 recognized all cd44 isoforms, and showed high sen ... | 2020 | 33000243 |