Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| evaluating the efficiency of chef and cmv promoter with ires and furin/2a linker sequences for monoclonal antibody expression in cho cells. | in recent years, monoclonal antibodies (mabs) have been developed as powerful therapeutic and diagnostic agents and chinese hamster ovary (cho) cells have emerged as the dominant host for the recombinant expression of these proteins. a critical step in recombinant expression is the utilization of strong promoters, such as the chinese hamster elongation factor-1α (chef-1) promoter. to compare the strengths of chef with cytomegalovirus (cmv) promoter for mab expression in cho cells, four bicistron ... | 2017 | 29023479 |
| proline-rich chaperones are compared computationally and experimentally for their abilities to facilitate recombinant butyrylcholinesterase tetramerization in cho cells. | human butyrylcholinesterase (bche), predominantly tetramers with a residence time of days, offers the potential to scavenge organophosphorus pesticides and chemical warfare agents. efficient assembly of human bche into tetramers requires an association with proline-rich peptide chaperones. in this study, the incorporation of different proline-rich peptide chaperones into bche is investigated computationally and experimentally. first, the authors applied molecular dynamic (md) simulations to inte ... | 2017 | 29024569 |
| kinetic characterization of bile salt transport by human ntcp (slc10a1). | the transport of bile acids facilitated by ntcp is an important factor in establishing bile flow. in this study, we examine the kinetics associated with human ntcp-dependent transport of two quantitatively important bile acids comprising the human bile acid pool, chenodeoxycholic acid and glycine-chenodeoxycholate, and secondary bile salt, 3-sulfo-glycolithocholate of potential toxicological significance. the study employed human ntcp overexpressing chinese hamster ovary cells and results compar ... | 2018 | 29024779 |
| types of cell death and apoptotic stages in chinese hamster ovary cells distinguished by raman spectroscopy. | cell death is the ultimate cause of productivity loss in bioreactors that are used to produce therapeutic proteins. we investigated the ability of raman spectroscopy to detect the onset and types of cell death for chinese hamster ovary (cho) cells-the most widely used cell type for therapeutic protein production. raman spectroscopy was used to compare apoptotic, necrotic, autophagic, and control cho cells. several specific nucleic acid-, protein-, and lipid-associated marker bands within the 650 ... | 2018 | 29030978 |
| construction and expression of vectors encoding biologically active rodent gonadotropins. | the gonadotropins, luteinizing hormone (lh) and follicle-stimulating hormone (fsh), are important hormones in vertebrate reproduction. the isolation of gonadotropins from the pituitary gland is sub-optimal, as the cross-contamination of one hormone with another is common and often results in the variation in the measured activity of lh and fsh. the production of recombinant hormones is, therefore, a viable approach to solve this problem. this study aimed to express recombinant rat, mouse, and ma ... | 2017 | 29033405 |
| an investigation of methyl tert‑butyl ether‑induced cytotoxicity and protein profile in chinese hamster ovary cells. | methyl tert-butyl ether (mtbe) is widely used as an oxygenating agent in gasoline to reduce harmful emissions. however, previous studies have demonstrated that mtbe is a cytotoxic substance that has harmful effects in vivo and in vitro. although remarkable progress has been made in elucidating the mechanisms underlying the mtbe‑induced reproductive toxicological effect in different cell lines, the precise mechanisms remain far from understood. the present study aimed to evaluate whether mammalia ... | 2017 | 29039499 |
| wavelet imaging on multiple scales (wims) reveals focal adhesion distributions, dynamics and coupling between actomyosin bundle stability. | we introduce and use wavelet imaging on multiple scales (wims) as an improvement to fluorescence correlation spectroscopy to measure physical processes and features that occur across multiple length scales. in this study, wavelet transforms of cell images are used to characterize molecular dynamics at the cellular and subcellular levels (i.e. focal adhesions). we show the usefulness of the technique by applying wims to an image time series of a migrating osteosarcoma cell expressing fluorescentl ... | 2017 | 29049414 |
| hib antibody responses in infants following diphtheria, tetanus, acellular pertussis, and conjugated haemophilus influenzae type b (hib) combination vaccines with decreasing amounts of tetanus toxoid. | while combination vaccines have contributed to improved vaccine uptake rates in children, studies have documented varied immunogenicity to specific vaccine components. we studied whether varying the amount of tetanus toxoid (tt) in a dtap and hib combination vaccine would result in immunogenicity comparable with separate, concurrent administration. | 2017 | 29054729 |
| potentiation and block of asic1a by memantine. | acid-sensing ion channels (asics) are modulated by various classes of ligands, including the recently described hydrophobic monoamines, which inhibit and potentiate asics in a subunit-specific manner. in particular, memantine inhibits asic1a and potentiates asic2a homomers. the aim of the present work was to characterize action mechanism of memantine on recombinant asic1a expressed in cho (chinese hamster ovary) cells. we have demonstrated that effect of memantine on asic1a strongly depends on m ... | 2017 | 29058095 |
| ca2+ -dependent down-regulation of human histamine h1 receptors in chinese hamster ovary cells. | gq/11 protein-coupled human histamine h1 receptors in chinese hamster ovary cells stimulated with histamine undergo clathrin-dependent endocytosis followed by proteasome/lysosome-mediated down-regulation. in this study, we evaluated the effects of a sustained increase in intracellular ca2+ concentrations induced by a receptor-bypassed stimulation with ionomycin, a ca2+ ionophore, on the endocytosis and down-regulation of h1 receptors in chinese hamster ovary cells. all cellular and cell-surface ... | 2018 | 29063596 |
| multimodal chromatography for purification of biotherapeutics - a review. | process chromatography forms the core of purification of biotherapeutics. the unparalleled selectivity that it offers over other alternatives combined with the considerable robustness and scalability make it the unit operation of choice in downstream processing. it is typical to have three to five chromatography steps in a purification process for a biotherapeutic. generally, these steps offer different modes of separation such as ion-exchange, reversed phase, size exclusion, and hydrophobic int ... | 2017 | 29065828 |
| z-505 hydrochloride ameliorates chemotherapy-induced anorexia in rodents via activation of the ghrelin receptor, ghsr1a. | despite its therapeutic advantages, chemotherapy with anti-cancer drugs can cause adverse effects, including anorexia and weight loss. although most patients with cancer suffer from anorexia during chemotherapy, resulting in the need to suspend or cease treatment and thereby worsening prognosis, treatment options for anorexia remain limited. ghrelin is an orexigenic hormone that has been proposed to prevent anorexia. to investigate the potential of ghrelin receptor agonists, synthetic small-mole ... | 2018 | 29066414 |
| regulatory role of mir-18a to ccn2 by tgf-β1 signaling pathway in pulmonary injury induced by nano-sio2. | this research is designed to investigate the regulatory effect of mir-18a to the target gene connective tissue growth factor (ctgf, or ccn2), by participating in tgf-β1 signaling pathway and explore the pathogenic mechanism of mir-18a in pulmonary injury induced by nano-sio2 based on our early study. mir-18a and expression of tgf-β1 in chinese hamster lung (chl) fibroblasts cells stimulated by supernatants of nr8383 cells exposed to 40 μg/ml nano-sio2 for 24 h demonstrated 1.58 ± 0.22-fold and 1 ... | 2018 | 29067610 |
| a possible link between kcnq2- and stxbp1-related encephalopathies: stxbp1 reduces the inhibitory impact of syntaxin-1a on m current. | kv7 channels mediate the voltage-gated m-type potassium current. reduction of m current due to kcnq2 mutations causes early onset epileptic encephalopathies (eoees). mutations in stxbp1 encoding the syntaxin binding protein 1 can produce a phenotype similar to that of kcnq2 mutations, suggesting a possible link between stxbp1 and kv7 channels. these channels are known to be modulated by syntaxin-1a (syn-1a) that binds to the c-terminal domain of the kv7.2 subunit and strongly inhibits m current. ... | 2017 | 29067685 |
| nanoliposomes for safe and efficient therapeutic mrna delivery: a step toward nanotheranostics in inflammatory and cardiovascular diseases as well as cancer. | rationale: genetic therapy using modified mrna for specific therapeutic protein expression for disease treatment and vaccination represents a new field of therapeutic and diagnostic medicine. non-viral vectors transfection using biocompatible nanoliposomes enables safe and efficient delivery of therapeutic mrna. objective: generation of non-toxic, cell-compatible cationic nanoliposomes as nanotheranostic agents to successfully deliver therapeutic mrna. methods and results: cationic nanoliposomes ... | 2017 | 29071184 |
| cho-omics review: the impact of current and emerging technologies on chinese hamster ovary based bioproduction. | cho cells are the most prevalent platform for modern bio-therapeutic production. currently, there are several cho cell lines used in bioproduction with distinct characteristics and unique genotypes and phenotypes. these differences limit advances in productivity and quality that can be achieved by the most common approaches to bioprocess optimization and cell line engineering. incorporating omics-based approaches into current bioproduction processes will complement traditional methodologies to m ... | 2017 | 29072373 |
| identification of a potent mar element from the human genome and assessment of its activity in stably transfected cho cells. | low-level and unstable transgene expression are common issues using the cho cell expression system. matrix attachment regions (mars) enhance transgene expression levels, but additional research is needed to improve their function and to determine their mechanism of action. mar-6 from cho chromosomes actively mediates high and consistent gene expression. in this study, we compared the effects of two new mars and mar-6 on transgene expression in recombinant cho cells and found one potent mar eleme ... | 2018 | 29077269 |
| metabolic phenotyping of cho cells varying in cellular biomass accumulation and maintenance during fed-batch culture. | cho cell lines capable of high-level recombinant protein product biosynthesis during fed-batch culture are still generally obtained by intensive empirical screening of transfected cells rather than knowledge-guided cellular engineering. in this study, we investigate how cho cell lines create and maintain cellular biosynthetic capacity during fed-batch culture to achieve the optimal combination of rapid exponential proliferation and extended maintenance of high cell biomass concentration. we perf ... | 2017 | 29080362 |
| human endoglin as a potential new partner involved in platelet-endothelium interactions. | complex interactions between platelets and activated endothelium occur during the thrombo-inflammatory reaction at sites of vascular injuries and during vascular hemostasis. the endothelial receptor endoglin is involved in inflammation through integrin-mediated leukocyte adhesion and transmigration; and heterozygous mutations in the endoglin gene cause hereditary hemorrhagic telangiectasia type 1. this vascular disease is characterized by a bleeding tendency that is postulated to be a consequenc ... | 2017 | 29080903 |
| selenium and zinc against aβ25-35-induced cytotoxicity and tau phosphorylation in pc12 cells and inhibits γ-cleavage of app. | amyloid beta (aβ) is the main component of the amyloid plaques that accumulate in the brains of alzheimer patients. the present study was conducted to investigate whether the combined treatment with selenium (se) and zinc (zn) offers more beneficial effects than that provided by either of them alone in reversing aβ25-35-induced neurotoxicity in pc12 cells. cells were pretreated with 0.1 μmol/l of se and zn for 4 h, after treated with 10 mmol/l aβ25-35 for 24 h. cells were divided into control an ... | 2017 | 29081063 |
| fluorescent turn-on probes for wash-free mrna imaging via covalent site-specific enzymatic labeling. | investigating the many roles rna plays in cellular regulation and function has increased demand for tools to explore rna tracking and localization within cells. our recently reported rna-tag (transglycosylation at guanine) approach uses an rna-modifying enzyme, trna-guanine transglycosylase (tgt), to accomplish covalent labeling of an rna of interest with fluorescent tracking agents in a highly selective and efficient manner. unfortunately, labeling by this method currently suffers from a high n ... | 2017 | 29081948 |
| the dietary furocoumarin imperatorin increases plasma glp-1 levels in type 1-like diabetic rats. | imperatorin, a dietary furocoumarin, is found not only in medicinal plants, but also in popular culinary herbs, such as parsley and fennel. recently, imperatorin has been shown to activate gpr119 in cells. another gpr, gpr131, also called tgr5 or g-protein-coupled bile acid receptor 1 (gpbar1), is known to regulate glucose metabolism. additionally, tgr5 activation increases glucagon-like peptide (glp-1) secretion to lower blood sugar levels in animals. therefore, the present study aims to determ ... | 2017 | 29084156 |
| magnesium stearate, a widely-used food additive, exhibits a lack of in vitro and in vivo genotoxic potential. | magnesium stearate is widely used in the production of dietary supplement and pharmaceutical tablets, capsules and powders as well as many food products, including a variety of confectionery, spices and baking ingredients. although considered to have a safe toxicity profile, there is no available information regarding its potential to induce genetic toxicity. to aid safety assessment efforts, magnesium sulfate was evaluated in a battery of tests including a bacterial reverse mutation assay, an i ... | 2017 | 29090120 |
| cloning and expression of recombinant human interleukin-7 in chinese hamster ovary (cho) cells. | the critical role of interleukin-7 (il-7) in homeostatic proliferation and t cell survival has made it a promising cytokine for the treatment of various clinical conditions, especially those associated with lymphopenia. | 2017 | 29090231 |
| construction of mtb72f plasmid as a dna vaccine candidate for mycobacterium tuberculosis. | with one-third of the world's population infected, tuberculosis (tb) is one of the most common infectious diseases and a major public health problem, especially in developing countries. the efficacy of the bcg vaccine for controlling the disease in adults is poor. the development of an effective tb vaccine is a global objective. an effective tuberculosis vaccine should stimulate cellular immunity. dna vaccines are a new generation of vaccines with the potential to achieve this goal. the aim of t ... | 2017 | 29090235 |
| optimized selection marker and cho host cell combinations for generating high monoclonal antibody producing cell lines. | there are several selection markers which are suitable for generating stably transfected chinese hamster ovary (cho) cell lines. due to their different modes of action, each selection marker has its own optimal selection stringency in different host cells for obtaining high productivity. using an internal ribosome entry site (ires)-mediated tricistronic vector and a set of ires variants with different strengths, the expression of five antibiotics resistance genes (args) in cho-k1 cells and dihyd ... | 2017 | 29090854 |
| a novel fc-engineered human icam-1/cd54 antibody with potent anti-myeloma activity developed by cellular panning of phage display libraries. | to identify antibodies suitable for multiple myeloma (mm) immunotherapy, a cellular screening approach was developed using plasma cell lines jk-6l and ina-6 and human synthetic single-chain fragment variable (scfv) phage libraries. isolated phage antibodies were screened for myeloma cell surface reactivity. due to its binding characteristics, phage piii-15 was selected to generate the scfv-fc fusion protein tp15-fc with an fc domain optimized for fcγriiia binding. various mm cell lines and patie ... | 2017 | 29100408 |
| chemical characterization and cytotoxic, genotoxic, and mutagenic properties of baccharis trinervis (lam, persoon) from colombia and brazil. | baccharis trinervis (lam, persoon) leaves are used in the traditional medicine for the treatment of high fevers, edema, inflammation, sores and muscle cramps, snakebites and as antiseptic. | 2018 | 29100934 |
| functional characterization of the dual allatostatin-a receptors in mosquitoes. | the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ... | 2018 | 29103918 |
| cell recognition molecule l1 regulates cell surface glycosylation to modulate cell survival and migration. | background: cell recognition molecule l1 (l1) plays an important role in cancer cell differentiation, proliferation, migration and survival, but its mechanism remains unclear. methodology/principal: our previous study has demonstrated that l1 enhanced cell survival and migration in neural cells by regulating cell surface glycosylation. in the present study, we show that l1 affected cell migration and survival in cho (chinese hamster ovary) cell line by modulation of sialylation and fucosylation ... | 2017 | 29104485 |
| glycan profile of cho derived igm purified by highly efficient single step affinity chromatography. | immunoglobulin m (igm) antibodies are reckoned as promising tools for therapy and diagnostic approaches. nevertheless, the commercial success of igms is hampered due to bottlenecks in recombinant production and downstream processing. igms are large, complex and highly glycosylated proteins that are only stable in a limited range of conditions. to investigate these sensitive igm antibodies we optimized the elution conditions for a commercially available igm affinity matrix (captureselect™). apply ... | 2017 | 29106908 |
| evaluating the toxicity of tio2-based nanoparticles to chinese hamster ovary cells and escherichia coli: a complementary experimental and computational approach. | titania-supported palladium, gold and bimetallic nanoparticles (second-generation nanoparticles) demonstrate promising photocatalytic properties. however, due to unusual reactivity, second-generation nanoparticles can be hazardous for living organisms. considering the ever-growing number of new types of nanoparticles that can potentially contaminate the environment, a determination of their toxicity is extremely important. the main aim of presented study was to investigate the cytotoxic effect o ... | 2017 | 29114443 |
| quantifying the potential for bursting bubbles to damage suspended cells. | bubbles that rise to the surface of a cell suspension can damage cells when they pop. this phenomenon is particularly problematic in the biotechnology industry, as production scale bioreactors require continuous injection of oxygen bubbles to maintain cell growth. previous studies have linked cell damage to high energy dissipation rates (edr) and have predicted that for small bubbles the edr could exceed values that would kill many cells used in bioreactors, including chinese hamster ovary (cho) ... | 2017 | 29118382 |
| tolerability, response and outcome of high-risk neuroblastoma patients treated with long-term infusion of anti-gd2 antibody ch14.18/cho. | immunotherapy with short term infusion (sti) of monoclonal anti-gd2 antibody (mab) ch14.18 (4 × 25 mg/m2/d; 8-20 h) in combination with cytokines and 13-cis retinoic acid (ra) prolonged survival in high-risk neuroblastoma (nb) patients. here, we investigated long-term infusion (lti) of ch14.18 produced in chinese hamster ovary cells (ch14.18/cho; 10 × 10 mg/m2; 24 h) in combination with subcutaneous (s.c.) interleukin-2 (il-2) in a single center program and report clinical response, toxicity and ... | 2018 | 29120699 |
| using specific recombinant gonadotropins to induce spermatogenesis and spermiation in the european eel (anguilla anguilla). | new specific european eel (anguilla anguilla) recombinant gonadotropins (aargths) produced in the ovarian cells of chinese hamsters (cho) were used to induce maturation in captive male eels. in the first experiment, five different hormonal treatments were assayed: one group was given a constant dose of recombinant european eel follicle-stimulating hormone (aarfsh; 4 μg/fish) for 9 weeks, and the second group received a constant dose of recombinant european eel luteinizing hormone (aarlh; 2 μg/fi ... | 2018 | 29120707 |
| towards replacement of the acellular pertussis vaccine safety test: comparison of in vitro cytotoxic activity and in vivo activity in mice. | because of the exquisite sensitivity of the murine histamine sensitization test (hist) in detecting minute amounts of active pertussis toxin (ptx), this animal-based test has been used to assure the safety of acellular pertussis vaccines in the united states and other countries around the world. prompted by humane considerations, efforts are underway to find a suitable in vitro replacement assay that has critical attributes comparable to that of the hist. in this study, we compared the sensitivi ... | 2017 | 29122386 |
| baicalein reduces oxidative stress in cho cell cultures and improves recombinant antibody productivity. | oxidative stress that naturally accumulates in the endoplasmic reticulum (er) as a result of mitochondrial energy metabolism and protein synthesis can disturb the er function. because er have a responsibility on the protein synthesis and quality control of the secreted proteins, er homeostasis has to be well maintained. when h2 o2 , an oxidative stress inducer, is added to recombinant chinese hamster ovary (rcho) cell cultures, it reduced cell growth, monoclonal antibody (mab) production, and ga ... | 2017 | 29125225 |
| characterizing steady states of genome-scale metabolic networks in continuous cell cultures. | in the continuous mode of cell culture, a constant flow carrying fresh media replaces culture fluid, cells, nutrients and secreted metabolites. here we present a model for continuous cell culture coupling intra-cellular metabolism to extracellular variables describing the state of the bioreactor, taking into account the growth capacity of the cell and the impact of toxic byproduct accumulation. we provide a method to determine the steady states of this system that is tractable for metabolic netw ... | 2017 | 29131817 |
| dietary k+ and cl- independently regulate basolateral conductance in principal and intercalated cells of the collecting duct. | the renal collecting duct contains two distinct cell types, principal and intercalated cells, expressing potassium kir4.1/5.1 (kcnj10/16) and chloride clc-k2 (clc-kb in humans) channels on their basolateral membrane, respectively. both channels are thought to play important roles in controlling systemic water-electrolyte balance and blood pressure. however, little is known about mechanisms regulating activity of kir4.1/5.1 and clc-k2/b. here, we employed patch clamp analysis at the single channe ... | 2018 | 29134279 |
| proteomic analysis of cho cell lines producing high and low quantities of a recombinant antibody before and after selection with methotrexate. | high levels of recombinant protein production in chinese hamster ovary (cho) cells can be achieved by amplification of transgenes using the dihydrofolate reductase/methotrexate (dhfr/mtx) system. with the aim to identify predictive markers enabling the preselection of suitable high producing clones we investigated the impact of mtx-based gene amplification on two cho cells lines producing different levels of a human monoclonal antibody by carrying out a comparative proteome analysis. the differe ... | 2018 | 29137976 |
| mild heat induces a distinct "eustress" response in chinese hamster ovary cells but does not induce heat shock protein synthesis. | the current research on cellular heat stress management focuses on the roles of heat shock proteins (hsps) and the proteostasis network under severe stress conditions. the mild, fever-type stress and the maintenance of membrane homeostasis are less well understood. herein, we characterized the acute effect of mild, fever-range heat shock on membrane organization, and hsp synthesis and localization in two mammalian cell lines, to delineate the role of membranes in the sensing and adaptation to he ... | 2017 | 29142280 |
| inhibition of glycation-induced cytotoxicity, protein glycation, and activity of proteolytic enzymes by extract from perovskia atriplicifolia roots. | protein glycation and glycotoxicity belong to the main oxidative-stress related complications in diabetes. perovskia species are used in asian folk medicine as antidiabetic herbs. | 2017 | 29142432 |
| calcein release from cells in vitro via reversible and irreversible electroporation. | the aim of this study was to investigate the dependence of calcein extraction and cell viability on the parameters of pulsed electric field (pef). two different approaches concerning pef parameters were investigated: (1) extraction efficiency and cell viability dependence on pulse number, exploiting 1200 v/cm 100 µs duration high voltage (hv) electric pulses and (2) extraction efficiency and cell viability dependence on the pulses with different duration (44-400 µs) and electric field strength ( ... | 2017 | 29143077 |
| identification, synthesis, and safety assessment of thidiazuron [1-phenyl-3-(1,2,3-thidiazol-5-yl)urea] and its metabolites in kiwifruits. | the quality of kiwifruit became worse as a result of the abuse of plant growth regulators (pgrs). the safety of the fruits treated with pgrs also worried consumers. therefore, the present study analyzed the structure of thidiazuron [tdz, (1-phenyl-3-(1,2,3-thidiazol-5-yl)-urea)] (1) and its metabolites of biotransformation in kiwifruits using liquid chromatography hybrid ion trap time-of-flight mass spectrometry (lc-it-tof-ms). standard compounds were also synthesized and used for structural ide ... | 2017 | 29144740 |
| engineered and natural promoters and chromatin-modifying elements for recombinant protein expression in cho cells. | promoters play a pivotal role in integrating and processing the signals related to transcription initiation. strong natural viral promoters, such as hcmv or sv40e, have been routinely employed to achieve a high rate of gene expression in ubiquitously used chinese hamster ovary (cho) cells. however, viral promoters are susceptible to epigenetic silencing and lack precise regulation levers. this has paved the way to more sensible control elements: endogenous, inducible, and synthetic promoters. in ... | 2017 | 29145694 |
| novel intracellular transport-refractory mutations in kcnh2 identified in patients with symptomatic long qt syndrome. | missense mutations in kcnh2, a gene encoding the kv11.1 channel, cause long qt syndrome (lqts) type 2 primarily by disrupting the intracellular transport of kv11.1 to the plasma membrane. the present study aimed to clarify the functional changes by two novel kcnh2 missense mutations. | 2017 | 29146210 |
| sustaining an efficient and effective cho cell line development platform by incorporation of 24-deep well plate screening and multivariate analysis. | efficient and effective cell line screening is paramount toward a successful biomanufacturing program. here we describe the implementation of 24-deep well plate (24-dwp) screening of cho lines as part of the cell line development platform at abbvie. incorporation of this approach accelerated the identification of the best candidate lines for process development. in an effort to quantify and predict process performance comparability, we compared cell culture performance in and in shake flasks, fo ... | 2017 | 29150912 |
| genotoxicity and 28-day oral toxicity studies of a functional food mixture containing maltodextrin, white kidney bean extract, mulberry leaf extract, and niacin-bound chromium complex. | steady-fiber granule (sfg) is a functional food mixture that is composed of four major ingredients, resistant maltodextrin, white kidney bean (phaseolus vulgaris) extract, mulberry leaf (morus alba l.) extract, and niacin-bound chromium complex. this study focused on determining the safety of sfg. genotoxicity and 28-day oral toxicity were evaluated. sfg did not induce mutagenicity in the bacterial reverse mutation assay using five salmonella typhimurium strains (ta98, ta100, ta102, ta1535, and ... | 2017 | 29155076 |
| a novel mutation in the fibrinogen bβ chain (c.490g>a; end of exon 3) causes a splicing abnormality and ultimately leads to congenital hypofibrinogenemia. | we found a novel heterozygous mutation in the fibrinogen bβ chain (c.490g>a) of a 3-year-old girl with congenital hypofibrinogenemia. to clarify the complex genetic mechanism, we made a mini-gene including a fgb c.490g>a mutation region, transfected it into a chinese hamster ovary (cho) cell line, and analyzed reverse transcription (rt) products. the assembly process and secretion were examined using recombinant mutant fibrinogen. direct sequencing demonstrated that the mutant rt product was 99 ... | 2017 | 29156616 |
| an endogenous retroviral envelope syncytin and its cognate receptor identified in the viviparous placental mabuya lizard. | syncytins are envelope genes from endogenous retroviruses that have been captured during evolution for a function in placentation. they have been found in all placental mammals in which they have been searched, including marsupials. placental structures are not restricted to mammals but also emerged in some other vertebrates, most frequently in lizards, such as the viviparous mabuya scincidae. here, we performed high-throughput rna sequencing of a mabuya placenta transcriptome and screened for t ... | 2017 | 29162694 |
| formulation and characterization of poly(propylacrylic acid)/poly(lactic-co-glycolic acid) blend microparticles for ph-dependent membrane disruption and cytosolic delivery. | poly(lactic-co-glycolic acid) (plga) is widely used as a vehicle for delivery of pharmaceutically relevant payloads. plga is readily fabricated as a nano- or microparticle (mp) matrix to load both hydrophobic and hydrophilic small molecular drugs as well as biomacromolecules such as nucleic acids and proteins. however, targeting such payloads to the cell cytosol is often limited by mp entrapment and degradation within acidic endolysosomes. poly(propylacrylic acid) (ppaa) is a polyelectrolyte pol ... | 2017 | 29164777 |
| role of 6-o-α-maltosyl-β-cyclodextrin in lysosomal cholesterol deprivation in npc1-deficient chinese hamster ovary cells. | we aimed to investigate whether 6-o-α-maltosyl-β-cyclodextrin (mal-βcd) is incorporated into cells and lysosomes during the release of unesterified cholesterol in npc1-deficient chinese hamster ovary (cho) cells (npc1 ko cells) and cho-jp17 cells (jp17 cells). internalization of mal-βcd in cells and lysosomes and extracellular release of lysosomal unesterified cholesterol were demonstrated by lc/ms/ms and lc/ms, respectively. internalization of mal-βcd was greater in npc1 ko cells than in jp17 c ... | 2018 | 29172126 |
| astrocytic γ-aminobutyric acid (gaba) transporters mediate guanidinoacetate transport in rat brain. | guanidinoacetate (gaa) is a biosynthetic precursor of creatine, which plays a critical role in homeostasis of high-energy phosphates in the brain, but cerebral accumulation of gaa leads to neurological complications, such as epilepsy and seizures. the purpose of the present study was to clarify the contribution of the γ-aminobutyric acid (gaba) transport systems to gaa transport in astrocytes by means of uptake studies in rat brain slices, primary astrocyte cultures and chinese hamster ovary (ch ... | 2017 | 29175673 |
| combinations of chromosome transfer and genome editing for the development of cell/animal models of human disease and humanized animal models. | chromosome transfer technology, including chromosome modification, enables the introduction of mb-sized or multiple genes to desired cells or animals. this technology has allowed innovative developments to be made for models of human disease and humanized animals, including down syndrome model mice and humanized transchromosomic (tc) immunoglobulin mice. genome editing techniques are developing rapidly, and permit modifications such as gene knockout and knockin to be performed in various cell li ... | 2018 | 29180645 |
| observability of complex systems: finding the gap. | for a reconstruction of state and parameter values in a dynamic system model, first the question whether these values can be uniquely determined from the data must be answered. this structural model property is known as observability or, in case of parameter calibration only, identifiability. testing a given model for observability is a well studied problem in the systems and control sciences. however, it is increasingly difficult, if not impossible, to address this property for large size model ... | 2017 | 29185491 |
| enhanced igg1 production by overexpression of nuclear factor kappa b inhibitor zeta (nfkbiz) in chinese hamster ovary cells. | several engineering strategies have been employed to improve the production of therapeutic recombinant proteins in chinese hamster ovary (cho) cell lines. we have focused on unfolded protein response-based engineering and reported that atf4 overexpression increases protein production. in this study, transcriptome analysis of atf4-overexpressed cho cells was performed using high-coverage expression profiling, to search for another key factor contributing to recombinant protein production. we obse ... | 2017 | 29188404 |
| amp and adenosine are both ligands for adenosine 2b receptor signaling. | adenosine is considered the canonical ligand for the adenosine 2b receptor (a2br). a2br is upregulated following kidney ischemia augmenting post ischemic blood flow and limiting tubular injury. in this context the beneficial effect of a2br signaling has been attributed to an increase in the pericellular concentration of adenosine. however, following renal ischemia both kidney adenosine monophosphate (amp) and adenosine levels are substantially increased. using computational modeling and calcium ... | 2018 | 29191553 |
| hypersensitivity of brca2 deficient cells to rosemary extract explained by weak parp inhibitory activity. | rosemary extract is used in food additives and traditional medicine and has been observed to contain anti-tumor activity. in this study, rosemary extract is hypothesized to induce synthetic lethality in brca2 deficient cells by parp inhibition. chinese hamster lung v79 cells and its mutant cell lines, v-c8 (brca2 deficient) and v-c8 with brca2 gene correction were used. rosemary extract and its major constituent chemicals were tested for their cytotoxicity by colony formation assay in cells of d ... | 2017 | 29196727 |
| chromosomal instability and cytotoxicity induced by ribavirin: comparative analysis in cell lines with different drug-metabolizing profiles. | ribavirin is an important component of the treatment for hepatitis c virus (hcv) infection and, in combination with the new direct-acting antiviral (daa) agents, comprises the major current therapeutic regimens. this study evaluated the cytotoxicity and chromosomal instability induced by ribavirin using the in vitro cytokinesis-block micronucleus cytome (cbmn-cyt) assay in two cell lines with different expression levels of drug-metabolizing enzymes: human hepatocellular carcinoma cells (hepg2) a ... | 2017 | 29199475 |
| absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases. | a recent publication suggested molecular mimicry of a nucleoprotein (np) sequence from a/puerto rico/8/1934 (pr8) strain, the backbone used in the construction of the reassortant strain x-179a that was used in pandemrix® vaccine, and reported on anti-hypocretin (hcrt) receptor 2 (anti-hcrtr2) autoantibodies in narcolepsy, mostly in post pandemrix® narcolepsy cases (17 of 20 sera). in this study, we re-examined this hypothesis through mass spectrometry (ms) characterization of pandemrix®, and two ... | 2017 | 29220370 |
| distinctive binding properties of the negative allosteric modulator, [3h]sb269,652, at recombinant dopamine d3 receptors. | recently, employing radioligand displacement and functional coupling studies, we demonstrated that sb269,652 (n-[(1r,4r)-4-[2-(7-cyano-1,2,3,4-tetrahydroisoquinolin-2-yl)ethyl]cyclohexyl]-1h-indole-2-carboxamide) interacts in an atypical manner with dopamine d3 receptor displaying a unique profile reminiscent of a negative allosteric ligand. here, we characterized the binding of radiolabelled [3h]sb269,652 to human dopamine d3 receptor stably expressed in chinese hamster ovary cells. under satur ... | 2018 | 29223348 |
| a chimeric hs4 insulator-scaffold attachment region enhances transgene expression in transfected chinese hamster ovary cells. | chinese hamster ovary (cho) cells are one of the most commonly used expression systems for the production of recombinant proteins but low levels of transgene expression and transgene silencing are frequently encountered. epigenetic regulatory elements such as the chicken β-globin locus control region hypersensitive site 4 (hs4) and scaffold/matrix attachment regions (s/mars) have positive effects on transgene expression. in this study, a chimeric hs4-sar was cloned upstream or downstream of an e ... | 2017 | 29226088 |
| probing the importance of clonality: single cell subcloning of clonally derived cho cell lines yields widely diverse clones differing in growth, productivity, and product quality. | in the past few decades, a large variety of therapeutic antibodies and proteins have been expressed in chinese hamster ovary (cho) cells. this mammalian expression system is robust, scalable, relatively inexpensive, and importantly allows for post-translational modifications that are important for some therapeutic proteins. historically, cho cell lines were derived from colonies of cells grown in semi-solid or liquid plates using either serum-containing or serum-free media. current advancements ... | 2017 | 29226566 |
| a "cell-friendly" window for the interaction of cells with hyaluronic acid/poly-l-lysine multilayers. | polyelectrolyte multilayers assembled from hyaluronic acid (ha) and poly-l-lysine (pll) are most widely studied showing excellent reservoir characteristics to host molecules of diverse nature; however, thick (ha/pll)n films are often found cell repellent. by a systematic study of the adhesion and proliferation of various cells as a function of bilayer number "n" a correlation with the mechanical and chemical properties of films is developed. the following cell lines have been studied: mouse 3t3 ... | 2017 | 29231289 |
| antiparkinsonian and antidyskinetic profiles of two novel potent and selective nociceptin/orphanin fq receptor agonists. | we previously showed that nociceptin/orphanin fq opioid peptide (nop) receptor agonists attenuate the expression of levodopa-induced dyskinesia in animal models of parkinson's disease. we now investigate the efficacy of two novel, potent and chemically distinct nop receptor agonists, at-390 and at-403, to improve parkinsonian disabilities and attenuate dyskinesia development and expression. | 2017 | 29232769 |
| development of hyper osmotic resistant cho host cells for enhanced antibody production. | cell culture platform processes are generally employed to shorten the duration of new product development. a fed-batch process with continuous feeding is a conventional platform process for monoclonal antibody production using chinese hamster ovary (cho) cells. to establish a simplified platform process, the feeding method can be changed from continuous feed to bolus feed. however, this change induces a rapid increase of osmolality by the bolus addition of nutrients. the increased osmolality sup ... | 2017 | 29233458 |
| toxicological evaluation of β-galactosidase enzyme produced by papiliotrema terrestris. | β-galactosidase (ec 3.2.1.23) is a glycoside hydrolase that catalyzes the release of sugar monomers from β-galactoside oligosaccharides via hydrolysis of glycosidic bonds, and has potential uses in the food industry. the safety of this enzyme and its production organism, papiliotrema terrestris (p. terrestris), are described herein. p. terrestris was non-pathogenic upon intravenous (iv) administration of 2.4 × 108 cfu and oral administration of 1.3 × 109 cfu. genotoxic results for the enzyme con ... | 2017 | 29233771 |
| abin-1 negatively regulates μ-opioid receptor function. | the μ-opioid receptor (mor) is a gi/o protein-coupled receptor that mediates analgesic, euphoric, and reward effects. using a bacterial two-hybrid screen, we reported that the carboxyl tail of the rat mor associates with a20-binding inhibitor of nuclear factor κb (abin-1). this interaction was confirmed by direct protein-protein binding and coimmunoprecipitation of mor and abin-1 proteins in cell lysates. saturation binding studies showed that abin-1 had no effect on mor binding. however, the in ... | 2018 | 29237725 |
| insights into the generation of monoclonal antibody acidic charge variants during chinese hamster ovary cell cultures. | charge variation is one of the most important heterogeneities during monoclonal antibody (mab) manufacturing and this study presents insights into the generation of acidic charge variants during cell culture processes. since acidic variants generate both intracellularly and extracellularly, main charge fraction collected by weak cation exchange chromatography (wcx) was incubated in harvested cell supernatant (hcs) to simulate and investigate the extracellular process firstly. it is found that th ... | 2018 | 29238873 |
| hypoxia and transforming growth factor-beta1 pathway activation promote chinese hamster ovary cell aggregation. | suspension cultivation is the preferred mode of operation for the large-scale production of many biologics. chinese hamster ovary (cho) cells are anchorage-dependent in origin, but they have been widely adapted to suspension culture. in suspension culture, formation of cho cell aggregates is a common phenomenon and compromises cell culture performance in multiple ways. to better understand the underlying mechanisms that regulate cell aggregation, we utilized cho-specific transcriptome profiling ... | 2017 | 29251349 |
| synthesis, spectral characterization, antibacterial and anticancer activity of some titanium complexes. | titanium complexes with and without labile groups have been synthesized by the reaction of titanium tetrachloride (ticl4) with n- containing ligands (1,2-diaminocyclohexane (dach), 1,10-phenanthroline (phen) and adamantylamine (ada), 2,2'-bipyridine (bipy), 4,4'-dimethyl-2,2'-bipyridine (bpme)) in 1:2 and 1:2:2 molar ratio respectively. the synthesized complexes have been characterized by different spectroscopic techniques i.e. elemental analysis, ft-ir, nmr (1h and 13c) and mass spectrometry. t ... | 2017 | 29256358 |
| cytotoxic, antioxidative, genotoxic and antigenotoxic effects of horchata, beverage of south ecuador. | "horchata" is an herbal mixture infusion consumed in southern ecuador; 66% of its plants are anti-inflammatory medicinal plant, and 51% are analgesics. anti-inflammatory substances can prevent carcinogenesis mediated by cytotoxic effects and can prevent dna damage. the aim of this study was to evaluate the cytotoxicity and apoptotic/antigenotoxic effects of horchata as well as its mechanism. | 2017 | 29258490 |
| haloacetic acid water disinfection byproducts affect pyruvate dehydrogenase activity and disrupt cellular metabolism. | the disinfection of drinking water has been a major public health achievement. however, haloacetic acids (haas), generated as byproducts of water disinfection, are cytotoxic, genotoxic, mutagenic, carcinogenic, and teratogenic. previous studies of monohaa-induced genotoxicity and cell stress demonstrated that the toxicity was due to inhibition of glyceraldehyde-3-phosphate dehydrogenase (gapdh), leading to disruption of cellular metabolism and energy homeostasis. dihaas and trihaas are also prod ... | 2018 | 29261292 |
| formation of cell membrane component domains in artificial lipid bilayer. | the lipid bilayer environment around membrane proteins strongly affects their structure and functions. here, we aimed to study the fusion of proteoliposomes (pls) derived from cultured cells with an artificial lipid bilayer membrane and the distribution of the pl components after the fusion. pls, which were extracted as a crude membrane fraction from chinese hamster ovary (cho) cells, formed isolated domains in a supported lipid bilayer (slb), comprising phosphatidylcholine (pc), phosphatidyleth ... | 2017 | 29263355 |
| manipulation of the sodium-potassium ratio as a lever for controlling cell growth and improving cell specific productivity in perfusion cho cell cultures. | perfusion processes typically require removal of a continuous or semi-continuous volume of cell culture in order to maintain a desired target cell density. for fast growing cell lines, the product loss from this stream can be upwards of 35%, significantly reducing the overall process yield. as volume removed is directly proportional to cell growth, the ability to modulate growth during perfusion cell culture production thus becomes crucial. leveraging existing media components to achieve such co ... | 2017 | 29278412 |
| identification of process conditions influencing protein aggregation in chinese hamster ovary cell culture. | protein aggregation of monoclonal antibodies (mabs) is a common phenomenon associated with the production of these biopharmaceuticals. these aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream processing steps to remove the higher molecular weight species are inevitable. a preferable approach is to reduce the level of aggregation during bioprocessing by a careful adjustment of critical process parameters. recently, new analytical methods enabled ... | 2017 | 29280480 |
| drug-induced immune thrombocytopenia: mapping of the drug binding site to the membrane-proximal region of platelet gpix. | drug-induced immune thrombocytopenia (dit) is a common complication of several medications, including commonly used antibiotics. the most widely studied dit is caused by quinine. in dit, antibodies predominantly bind to the platelet membrane glycoprotein (gp) ix in a drug-dependent fashion resulting in increased platelet clearance. binding of the sensitizing drug, such as quinine, to gpix has been proposed but is yet to be established. this work demonstrates that quinine is retained specifically ... | 2017 | 29286872 |
| antigenotoxicity properties of copaifera multijuga oleoresin and its chemical marker, the diterpene (-)-copalic acid. | in view of the biological activities and growing therapeutic interest in oleoresin obtained from copaifera multijuga, this study aimed to determine the genotoxic and antigenotoxic potential of this oleoresin (cmo) and its chemical marker, diterpene (-)-copalic acid (ca). the micronucleus (mn) assay in v79 cell cultures and the ames test were used for in vitro analyses, as well as mn and comet assays in swiss mice for in vivo analyses. the in vitro genotoxicity/mutagenicity results showed that ei ... | 2018 | 29286884 |
| evaluation of reversed-phase nano liquid chromatography conditions by using reversed-phase thin layer chromatography based on hansen solubility parameters for the analysis of amphiphilic glycosylsphingolipid transformations. | pulse chase analysis is often used in investigating dynamics of cellular substances. fluorescently labeled lactosyl sphingosine molecule is useful in chasing its transformation, however the analysis of such metabolites in attomole level is of extreme difficult due to the presence of large amount of endogenous amphiphilic molecules such as glycosphingolipids, sphingomyerin, and glycerophospholipids. nano lc suites for analyzing the attomole scale metabolites, therefore removal of endogenous subst ... | 2018 | 29290400 |
| comparative study of the effects of phosphatidylcholine rich in dha and epa on alzheimer's disease and the possible mechanisms in cho-app/ps1 cells and samp8 mice. | metabolic stress induced by a high-fat (hf) diet leads to cognitive dysfunction and aging. in the present study, chinese hamster ovary cells stably transfected with amyloid precursor protein (app) and presenilin 1 (ps1) (cho-app/ps1 cells) and samp8 mice fed with an hf diet were used to study the effects of docosahexaenoic acid (dha)-enriched phosphatidylcholine (dha-pc) and eicosapentaenoic acid (epa)-enriched phosphatidylcholine (epa-pc) on alzheimer's disease (ad) and the possible mechanisms ... | 2018 | 29292421 |
| targeted knock-in of an scfv-fc antibody gene into the hprt locus of chinese hamster ovary cells using crispr/cas9 and cris-pitch systems. | chinese hamster ovary (cho) cells have been used as host cells for the production of pharmaceutical proteins. for the high and stable production of target proteins, the transgene should be integrated into a suitable genomic locus of host cells. here, we generated knock-in cho cells, in which transgene cassettes without a vector backbone sequence were integrated into the hprt locus of the cho genome using clustered regularly interspaced short palindromic repeats (crispr)/cas9 and crispr-mediated ... | 2017 | 29295784 |
| the class i scavenger receptor cd163 promotes internalization of adamts13 by macrophages. | internalization of adamts13 by macrophages may contribute to its clearance from the circulation. here we investigated endocytic mechanisms that contribute to the uptake of adamts13 by macrophages. human monocyte-derived macrophages were used to monitor the uptake of fluorescently labeled recombinant adamts13 by flow cytometry. internalization of adamts13 was blocked upon addition of the cell-permeable dynamin inhibitor dynasore. partial blocking of adamts13 uptake was observed by using mannan; h ... | 2017 | 29296945 |
| blood coagulation factor x exerts differential effects on adenovirus entry into human lymphocytes. | it has been proposed that blood coagulation factors, principally factor x (fx), enhance the uptake of human adenovirus type 5 (ad5) into cultured epithelial cells by bridging the viral hexon capsid protein and cell-surface heparan sulphate proteoglycans (hspgs). we studied the effects of fx on ad transduction of lymphoid cell lines (nk92mi, a natural killer cell line; daudi, a b-cell line and jurkat, a t-cell line) as well as primary peripheral blood lymphocytes (pbl) and hela epithelial cells u ... | 2018 | 29301346 |
| in vitro binding and receptor-mediated activity of terlipressin at vasopressin receptors v1 and v2. | terlipressin, a synthetic, systemic vasoconstrictor with selective activity at vasopressin-1 (v1) receptors, is a pro-drug for the endogenous/natural porcine hormone [lys8]-vasopressin (lvp). we investigated binding and receptor-mediated cellular activities of terlipressin, lvp, and endogenous human hormone [arg8]-vasopressin (avp) at v1 and vasopressin-2 (v2) receptors. cell membrane homogenates of chinese hamster ovary cells expressing human v1 and v2 receptors were used in competitive binding ... | 2018 | 29302194 |
| antidepressant drug paroxetine blocks the open pore of kv3.1 potassium channel. | in patients with epilepsy, depression is a common comorbidity but difficult to be treated because many antidepressants cause pro-convulsive effects. thus, it is important to identify the risk of seizures associated with antidepressants. to determine whether paroxetine, a very potent selective serotonin reuptake inhibitor (ssri), interacts with ion channels that modulate neuronal excitability, we examined the effects of paroxetine on kv3.1 potassium channels, which contribute to highfrequency fir ... | 2018 | 29302214 |
| ecotoxicological potential of the biocides terbutryn, octhilinone and methylisothiazolinone: underestimated risk from biocidal pathways? | the use of biocides by industry, agriculture and households increased throughout the last two decades. many new applications with known substances enriched the variety of biocidal pollution sources for the aquatic environment. while agriculture was the major source for a long time, leaching from building facades and preservation of personal care and cleaning products was identified as new sources in the last few years. with the different usage forms of biocidal products the complexity of legisla ... | 2018 | 29306832 |
| evaluation of different vector design strategies for the expression of recombinant monoclonal antibody in cho cells. | monoclonal antibodies (mabs) have emerged as the most promising category of recombinant proteins due to their high efficiency for the treatment of a wide range of human diseases. the complex nature of mabs creates a great deal of challenges in both upstream and downstream manufacturing processes. proportional expression and correct folding and assembly of the light chain and heavy chain are required for efficient production of the mabs. in this regard, expression vector design has proven to have ... | 2018 | 29313429 |
| methods for using small non-coding rnas to improve recombinant protein expression in mammalian cells. | the ability to produce recombinant proteins by utilizing different "cell factories" revolutionized the biotherapeutic and pharmaceutical industry. chinese hamster ovary (cho) cells are the dominant industrial producer, especially for antibodies. human embryonic kidney cells (hek), while not being as widely used as cho cells, are used where cho cells are unable to meet the needs for expression, such as growth factors. therefore, improving recombinant protein expression from mammalian cells is a p ... | 2018 | 29315258 |
| glycoengineering in cho cells: advances in systems biology. | for several decades, glycoprotein biologics have been successfully produced from chinese hamster ovary (cho) cells. the therapeutic efficacy and potency of glycoprotein biologics are often dictated by their post translational modifications, particularly glycosylation, which unlike protein synthesis, is a non-templated process. consequently, both native and recombinant glycoprotein production generate heterogeneous mixtures containing variable amounts of different glycoforms. stability, potency, ... | 2018 | 29316325 |
| rna-seq highlights high clonal variation in monoclonal antibody producing cho cells. | the development of next-generation sequencing technologies has opened new opportunities to better characterize complex eukaryotic cells. chinese hamster ovary (cho) cells play a primary role in therapeutic protein production, with currently five of the top ten blockbuster drugs produced in cho. however, engineering superior cho cells with improved production features has had limited success to date and cell lines are still developed through the generation and screening of large strain pools. her ... | 2018 | 29316330 |
| effectiveness of aqueous and hydroalcoholic extracts of acanthospermum australe (loefl.) kuntze against diarrhea-inducing bacteria. | leaves and roots of acanthospermum australe (asteraceae) have been used in brazilian folk medicine for the treatment of various ailments including diarrhea, skin diseases, blennorrhagia, dyspepsia, parasitic worms and malaria. the aim of study was to characterize the chemical profiles of the aqueous and hydroalcoholic extracts of leaves and roots of a. australe, and to evaluate their antimicrobial activities against diarrhea-inducing bacteria (enterococcus faecalis, shigella dysenteriae and yers ... | 2018 | 29319752 |
| development of a chemically defined platform fed-batch culture media for monoclonal antibody-producing cho cell lines with optimized choline content. | a chemically defined platform basal medium and feed media were developed using a single chinese hamster ovary (cho) cell line that produces a monoclonal antibody (mab). cell line a, which showed a peak viable cell density of 5.9 × 106 cells/ml and a final mab titer of 0.5 g/l in batch culture, was selected for the platform media development. stoichiometrically balanced feed media were developed using glucose as an indicator of cell metabolism to determine the feed rates of all other nutrients. a ... | 2018 | 29322349 |
| multiplexed digital mrna expression analysis profiles system-wide changes in mrna abundance and responsiveness of upr-specific gene expression changes during batch culture of recombinant chinese hamster ovary cells. | the unfolded protein response (upr) signalling pathway is viewed as critical for setting the effectiveness of recombinant protein expression in cho cells. in this study, we used nanostring ncounter technology to study expression of a group of genes associated with cellular processes linked to upr activation under er stress and the changing environment of a batch culture. time course induction of er stress, using tunicamycin (tm), showed a group of genes such as chop, trb3, sqstm1, grp78 and herp ... | 2018 | 29323465 |
| sirt3 activator honokiol attenuates β-amyloid by modulating amyloidogenic pathway. | honokiol (poly-phenolic lignan from magnolia grandiflora) is a sirtuin-3 (sirt3) activator which exhibit antioxidant activity and augment mitochondrial functions in several experimental models. modern evidence suggests the critical role of sirt3 in the progression of several metabolic and neurodegenerative diseases. amyloid beta (aβ), the precursor to extracellular senile plaques, accumulates in the brains of patients with alzheimer's disease (ad) and is related to the development of cognitive i ... | 2018 | 29324783 |
| the b cell death function of obinutuzumab-hdel produced in plant (nicotiana benthamiana l.) is equivalent to obinutuzumab produced in cho cells. | plants have attracted attention as bio-drug production platforms because of their economical and safety benefits. the preliminary efficacy of zmapp, a cocktail of antibodies produced in n. benthamiana (nicotiana benthamiana l.), suggested plants may serve as a platform for antibody production. however, because the amino acid sequences of the fab fragment are diverse and differences in post-transcriptional processes between animals and plants remain to be elucidated, it is necessary to confirm fu ... | 2018 | 29324849 |
| substance p as a putative efferent transmitter mediates gabaergic inhibition in mouse taste buds. | capsaicin-mediated modulation on taste nerve responses is thought to be produced indirectly by actions of neuropeptides, e.g., calcitonin gene-related peptide (cgrp) and substance p (sp), onto taste cells implying playing a role in taste sensitivity. during processing gustatory information in taste buds, cgrp shapes peripheral taste signals via serotonergic signaling. the underlying assumption has been that sp exerts its effects on taste transmitter secretion in taste buds of mice. | 2018 | 29328505 |
| changes in chromosome counts and patterns in cho cell lines upon generation of recombinant cell lines and subcloning. | chinese hamster ovary cells are the number one production system for therapeutic proteins. a pre-requirement for their use in industrial production of biopharmaceuticals is to be clonal, thus originating from a single cell in order to be phenotypically and genomically identical. in the present study it was evaluated whether standard procedures, such as the generation of a recombinant cell line in combination with selection for a specific and stable phenotype (expression of the recombinant produc ... | 2018 | 29328552 |
| use of the real time xcelligence system for purposes of medical microbiology. | roche's xcelligence impedance-measuring instrument is one of a few commercially available systems of such type. according to the best knowledge of authors, instrument was tested so far only for eukaryotic cell research. the aim of this work was to estimate xcelligence suitability for the microbiological tests, including (i) measurement of morphological changes in eukaryotic cells as a result of bacterial toxin activity, (ii) measurement of bacterial biofilm formation and (iii) impact of antisept ... | 2012 | 29334048 |
| inhibition of apoptosis using exosomes in chinese hamster ovary cell culture. | animal cell culture technology for therapeutic protein production has shown significant improvement over the last few decades. chinese hamster ovary (cho) cells have been widely adapted for the production of biopharmaceutical drugs. in the biopharmaceutical industry, it is crucial to develop cell culture media and culturing conditions to achieve the highest productivity and quality. however, cho cells are significantly affected by apoptosis in the bioreactors, resulting in a substantial decrease ... | 2018 | 29337363 |
| generation and characterization of siglec-f-specific monoclonal antibodies. | siglec-f (sf) is a surface glycoprotein expressed by mouse eosinophils and induces caspase- and mitochondria-dependent apoptosis after engagement with its cognate ligand or specific antibodies. this targeting eosinophils by monoclonal antibodies may help diverse diseases associated with increased frequency of eosinophils including allergy and asthma. in this paper, production of murine and rat monoclonal antibodies (mabs) against siglec-f has been addressed. balb/c mice were immunized with sigle ... | 2017 | 29338152 |